SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xiaopeng Hu,Yide Xia,Yiwei Liu,Yanran Chen,Baizhao Zeng
Ochratoxin A (OTA) can cause various diseases due to its severe toxicity, so its detection in foods is very important. Herein, a ratiometric electrochemical sensor based on molecularly imprinted polymer (RECS-MIP) has been developed for OTA detection. The MIP is prepared by cyclic voltammetry ; during electropolymerization a magnetic field is introduced to regulate the MIP orientation for improving molecular recognition efficiency. A gold nanoparticles , ploy(ionic liquid) and flavin mononucleotide decorated carbon nanotubes - MoS 2 nanosheet composite (Au NPs/PIL-FMNS/CNT-MoS 2 ) is used as substrate to amplify signal and to provide reference signal. The peak current of Au NPs/PIL-FMNS/CNT-MoS 2 decreases with the increase of OTA concentration, while the peak current of OTA oxidation product increases. The ratio of the peak currents changes linearly with OTA concentration variation and is used for OTA quantification. Under the optimized conditions, the linear response range is 0.5–15 μM and the low limit of detection is 14 nM (S/N = 3). The sensor displays high selectivity and reproducibility and has good application potential; it has been applied for the OTA detection in spiked samples and satisfactory results are achieved.
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JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Yuan Wang,Lingxiu Liu,Fan Chen,Shaoyu Fang,Xiaohai Yan,Wushuang Bai,Qinglin Sheng,Yahong Yuan,Kewei Feng,Tianli Yue
This study developed two novel magnetic nanocomposites with immobilized porcine pancreatic lipase (PPL) for simultaneously removing patulin (PAT) and ochratoxin A (OTA)—two hazardous environmental contaminants—from fruit juices. All detoxification experiments were conducted in real fruit juice matrices (apple juice for PAT, grape juice for OTA) under ambient temperature (25 °C) with realistic contamination ranges. The results demonstrated MNP@UiO-66-(SH)₂@PPL achieved removal rates of 77.03% for PAT in apple juice and 45.03% for OTA in grape juice, while MNP@UiO-66-NH₂@PPL showed removal rates of 85.74% for OTA and 48.44% for PAT. LC-MS analysis indicated that PAT undergoes ring-opening to ascladiol, while OTA is hydrolyzed to phenylalanine and OTα. Molecular docking revealed that PPL spontaneously binds to both toxins via hydrogen bonding and hydrophobic interactions. CCK-8 assays confirmed low cytotoxicity of the materials and their degradation products, with no adverse impact on juice quality. The composites also exhibited magnetic recovery rates > 93%, enabling facile separation and recyclability. This work provides a safe, efficient, and reusable strategy for multi-mycotoxin decontamination with demonstrated environmental relevance.
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FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Bin Liu,Miaohuan Xiong,Wanlin Sun,Huipeng Wu,Jialu Li,Yuqi Wang,Qing Wang,Jian He,Xuelan Chen
Ochratoxin A (OTA) is a widespread mycotoxin contaminating cereals, grape products, coffee, and spices, threatening global food safety and human health. Efficient detoxification strategies are therefore urgently needed. Herein, an OTA-degrading bacterium, Terribacillus saccharophilus JX-8, was isolated from Chinese sauerkraut juice, which completely degraded 12.4 μM OTA within 12 h via amide bond hydrolysis to yield non-toxic ochratoxin α (OTα). A novel OTA amidohydrolase OtaH was identified, sharing only 33%–42% sequence identity with previously characterized homologs. OtaH exhibited high catalytic efficiency ( k cat / K m = 4028.57 mM −1 min −1 ) and remarkable thermostability, retaining >50% activity after incubation at 65 °C for 1 h. Moreover, 5 μg/mL OtaH achieved 64.9% and 94.6% degradation of OTA in grape juice and wheat flour, respectively, within 2 h at 25 °C, reducing residual OTA to 1.76 μg/L and 0.54 μg/kg. Notably, enzymatic treatment did not significantly alter pH, soluble solids content, titratable acidity, polyphenol content, or nutritional composition. This study provides a promising enzymatic strategy for OTA detoxification in food processing.
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Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2023
·
IF 2.9
Zhang Wensi,Cui Chuanjin,Hongshuo Chen,Zhang Xuechao,Du Junhui
In this study, a novel electrochemical aptamer sensor for detecting ochratoxin A (OTA) was constructed. First, a gold-copper alloy film was prepared via electrochemical deposition, and copper was selectively dissolved in constant potential mode for obtaining the nano-porous gold modified screen-printed carbon electrodes (NPG/SPCE). Then, 2-mercaptoethylamine was dropped on the NPG/SPCE surface and Au-S covalent bonds were formed for immobilizing the metal. Glutaraldehyde as cross-linking agent was added, which resulted in immobilization and attachment of PAMAM to the 2-mercaptoethylamine through the dehydration condensation reaction. During the preparation process, the nano-porous gold and PAMAM-modified layers were characterized by SEM, XRD, and IR spectroscopy, respectively. The characterization results showed that the nano-porous gold and PAMAM composite films were successfully modified. Finally, the OTA aptamer was cross-linked with PAMAM by glutaraldehyde to complete construction of the Apt/PAMAM/NPG/SPCE sensor. The electrochemical performance of this sensor was tested in ochratoxin A solutions with the DPV method. The results showed that the sensor’s reproducibility, stability, and specificity were good. The spiked recoveries in red wine ranged from 99.65%∼101.6%, with a linear range of 0.5 ng/mL∼20 ng/mL and a minimum detection limit of 0.141 ng/mL. Thus, the novel biosensor may provide a promising tool for the trace detection of OTA.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yan Guan,Peng-Bin Si,Tong Yang,Yuan Wu,Yun-Hui Yang,Rong Hu
Ochratoxin A (OTA) is a naturally occurring mycotoxin that poses serious threats, such as kidney damage, to human health. Therefore, we developed a DNA walker-based dual-signal electrochemical ratiometric platform for OTA detection, which could overlook the variations in environmental and instrumental factors and DNA load densities. Cobalt metal–organic frameworks (Co-MOFs) and toluidine blue were used as the electrochemical signal tag and internal reference probe, respectively. In the presence of OTA, this developed machine resulted in the DNA labelled-Co-MOFs far away from the electrode. Thus, Co-MOFs signal at −1.18 V decreased, while toluidine blue at −0.28 V increased. This proposed strategy has displayed superior sensitivity (limit of detection = 0.31 fg/mL, linear range = 1–50 ng/mL) and high reproducibility. The sensor was also applied for determining OTA content in red wine samples and the results were comparable to those of commercial enzyme-linked immunoassay kits with satisfactory results.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 11
·2405-2414
·
IF 3.286
Wang Chengke,Tan Rong,Li Jiangyu,Zhang Zexiang
In this paper, a fluorescent method was developed for ochratoxin A (OTA) detection that uses iron-doped porous carbon (MPC) and aptamer-functionalized nitrogen-doped graphene quantum dots (NGQDs-Apt) as probes. In this method, the adsorbance of the NGQDs-Apt on the MPC due to a π–π interaction between the aptamer and the MPC results in the quenching of the fluorescence of the NGQDs-Apt. However, since OTA interacts strongly with the aptamer, the presence of OTA leads to the detachment of the NGQDs-Apt from the MPC, resulting in the resumption of fluorescence from the NGQDs-Apt. When exonuclease I (Exo I) is also added to the solution, this exonuclease specifically digests the aptamer, leading to the release of the OTA back into the solution. This free OTA then interacts with another MPC–NGQDs-Apt system, inducing the release of more NGQDs into the solution, which enhances the fluorescent intensity compared to that of the system with no Exo I. Utilizing this behavior of OTA in the presence of NGQDs-Apt, it was possible to detect concentrations of OTA ranging from 10 to 5000 nM, with a limit of detection of 2.28 nM. Our method was tested by applying it to the detection of OTA in wheat and corn samples. This method has four advantages: (1) the magnetic porous carbon is easy to prepare, its porosity enhances its loading capacity for NGQDs, it highly efficiently quenches the fluorescence of the NGQDs, and its magnetic properties facilitate the separation of the MPC from other species in solution; (2) applying double magnetic separation decreases the background signal; (3) Exo I digests the free aptamer effectively, which allows the resulting free OTA to induce the release of more NGQDs-Apt, ultimately enhancing the fluorescent signal; and (4) the proposed method presented high sensitivity and a wide linear detection range. This method may prove helpful in food safety analysis and new biosensor development (achieved by using different aptamer sequences to that used in the present work).
Exonuclease I (Exo I)-assisted fluorescent method for ochratoxin A (OTA) detection using magnetic porous carbon (MPC), nitrogen-doped graphene quantum dots (NGQDs), and double magnetic separation
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BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Wei Chen,Chao Yan,Lin Cheng,Li Yao,Feng Xue,Jianguo Xu
In this paper, a novel ultrasensitive signal-on electrochemical aptasensor has been proposed for Ochratoxin A (OTA) assay based on DNA controlled layer-by-layer assembly of dual gold nanoparticle (AuNP) conjugates. To construct the aptasensor, the 1 st AuNP conjugate was prepared by simultaneous immobilization of the capture probe 2 (CP2) and bridge probe (BP) onto the AuNPs. Then, OTA aptamer was loaded onto 1 st AuNPs by hybridization with CP2. The 1 st AuNP conjugate can be further immobilized onto the electrode by hybridization between BP and capture probe 1 (CP1), which was pre-immobilized on Au electrode. The 2 nd AuNP conjugate was prepared by immobilization of ferrocene (Fc) tagged SH-signal probe (SSP). Due to the recognition between aptamer on 1 st AuNP conjugate and OTA, CP2 was reformed in the ssDNA state, which can be utilized as the anchor for immobilization of 2 nd AuNP conjugate for electrochemical signal reporting. Because of the high surface-to-volume ratio and good conductivity of AuNPs, this dual AuNPs assembled nanoarchitecture finally lead to greatly improved abilities to load a large number of Fc molecules and significantly amply the electrochemical response even at a low target concentration. Both qualitative and quantitative analysis of OTA were thus realized by differential pulse voltammetry (DPV) signals, resulting in an excellent detection limit of 0.001 ppb and a wide dynamic range from 0.001 to 500 ppb over 6 orders of magnitude. Moreover, the real sample analysis towards OTA spiked wine samples was favorable, implying a great potential for practical applications. We envision that this unique dual AuNP conjugate assembly strategy would pave a new avenue for the development of versatile signal amplified electrochemical aptasensors.
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Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 13
·1965-1972
·
IF 4.776
Xia Yu,Hongliang Song,Jing Huang,Yongxuan Chen,Ming Dai,Xucong Lin,Zenghong Xie
A novel hybrid affinity monolith with a high aptamer coverage density was developed via a highly efficient modification of aptamer@gold nanoparticles (Apt@AuNPs) on a well-controlled 3D skeletal POSS–polyethylenimine (PEI) matrix. The effects of amine reagents and the skeletal structures of the hybrid monoliths on the modification efficiency of Apt@AuNPs were studied, and the resultant Apt@AuNPs@POSS–PEI1200 monolith was proposed with a high coverage density of aptamer of up to 1413 pmol μL−1, which is 4–5 times more than the coverage density when modified with Apt@AuNPs that was reported previously. Characterization including morphology, spectra analysis, mechanical stability, binding capacity and specificity studies was also carried out. Significant specific recognition targeting of ochratoxin A (OTA) was achieved with a detection limit as low as 0.06 ng mL−1. High recoveries of OTA were achieved of 92.6 ± 1.3% to 94.7 ± 1.4% (n = 3), while the cross-reactivity towards analogues such as ochratoxin B (OTB) and aflatoxin B1 (AFB1) was weak. This highlights a facile approach to POSS-based affinity monoliths with a high aptamer coverage density for sensitive and selective recognition of a trace target (OTA).
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Rong Liu,Lingxuan Huang,Xuan Feng,Di Wang,Rasika Gunarathne,QingJun Kong,Jun Lu,Xueyan Ren
Aspergillus carbonarius , a common food-contaminating fungus, produces ochratoxin A (OTA) and poses a risk to human health. This study aimed to assess the inhibitory activity of tea tree essential oil and its main components, Terpene-4-ol (T4), α-terpineol (αS), and 3-carene (3C) against A. carbonarius . The study showed αS and T4 were the main antifungal components of tea tree essential oil, which primarily inhibit A. carbonarius growth through cell membrane disruption, reducing antioxidant enzyme activities (catalase, peroxidase, superoxide dismutase) and interrupting the tricarboxylic acid cycle. Furthermore, αS and T4 interacted with enzymes related to OTA biosynthesis. Molecular docking and molecular dynamics show that they bound mainly to P450 with a minimum binding energy of −7.232 kcal/mol, we infered that blocking the synthesis of OTA precursor OTβ. Our hypothesis was preliminarily verified by the detection of key substances in the OTA synthesis pathway. The results of UHPLC-QTOF-MS 2 analysis demonstrated that T4 achieved a degradation rate of 43 % for OTA, while αS reached 29.6 %, resulting in final breakdown products such as OTα and phenylalanine. These results indicated that α-terpinol and Terpene-4-ol have the potential to be used as naturally safe and efficient preservatives or active packaging to prevent OTA contamination.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Xincheng Chen,Zhenyun He,Sujia Jiao,Zhichang Sun,Sihang Zhang,Xing Liu
Ochratoxin A (OTA) seriously threatens public health and agricultural ecosystems, necessitating sensitive detection methods. In view of that traditional immunoassays rely on animal-derived antibodies and toxic substrates, we developed a dual-mode nanosensor integrating colorimetric and fluorescent detection via alkaline phosphatase (ALP)-mediated silver nanoparticles (AgNPs) growth and inner filter effect (IFE). The Ag + -triggered aggregation-induced emission (AIE) enhancement of gold nanoclusters (AuNCs) was investigated for constructing a dual-mode nanosensor. The nanosensor employs an OTA-specific nanobody-ALP fusion (Nb-ALP) to dephosphorylate ascorbic acid 2-phosphate into ascorbic acid (AA), which reduces Ag⁺ to abundant AgNPs with NaBH 4 -reduced AgNPs seeds. AgNPs amplify plasmonic absorbance for colorimetric ALP detection, while quenching Ag⁺@AuNC fluorescence via IFE for fluorescent detection. The nanosensor was further combined with the Nb-ALP-based immunoassay to develop a dual-mode nanosensor-powered enzyme immunoassay (DMN-EIA). The DMN-EIA exhibited detection limits of 0.14 ng/mL (colorimetric) and 0.28 ng/mL (fluorescent) with high selectivity for OTA, with spike recovery rates of 93.5 %–108.7 % and relative standard deviations not exceeding 20 %. Furthermore, the colorimetric (R 2 = 0.96) and fluorescent DMN-EIA (R 2 = 0.93) correlated well with high-performance liquid chromatography in detection of ten real pepper samples. Therefore, the developed dual-mode nanosensor and DMN-EIA represent reliable and promising tools for detecting OTA.
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BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Chengxi Zhu,Dong Liu,Yuye Li,Shuai Ma,Meng Wang,Tianyan You
The simultaneous detection of multiple mycotoxins in grains is significant due to the enhanced toxicity induced by their synergistic effects . In this work, a dual-ratiometric electrochemical aptasensing strategy for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. Here, an anthraquinone-2-carboxylic acid (AQ)-labelled complementary DNA (cDNA) was used to provide separate and specific binding sites to assemble the ferrocene-labelled AFB1 aptamer (Fc-Apt1) and methylene blue-labelled OTA aptamer (MB-Apt2). The target-induced current ratios of I Fc /I AQ and I MB /I AQ were then used to quantitatively relate to AFB1 and OTA, respectively. Following this principle, two types of aptasensors involving the hairpin DNA (hDNA) and linear single-stranded DNA (ssDNA) as the cDNA were fabricated for performance comparisons. The results revealed that hairpin DNA with a rigid 2D structure can greatly improve the assembly and recognition efficiency of the sensing interface, which makes the hDNA-based aptasensor possess high sensitivity, reliability and anti-interference ability. The hDNA-based aptasensor exhibited a detection range of 10-3000 pg mL −1 for AFB1 and 30-10000 pg mL −1 for OTA, respectively, with no observable cross-reactivity. Furthermore, the aptasensor was applied to analyze corn and wheat samples, and the reliability was validated by HPLC-MS/MS. Our work has presented a novel way for fabricating a high-performance aptasensor for simultaneous detection of multiple mycotoxins .
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Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 6
·1585-1595
·
IF 3.641
Chen Ben-Qi,Yang Meng,Liao Wen-Chun,Li Zi-Ying,Chen Zhi-Xiong,Yang Yun-Hui,Yang Tong,Hu Rong
Developing a strategy for the determination of trace ochratoxin A (OTA) in food is very important to ensure food safety. Here, based on a tripod probe, an electrochemical aptasensing platform for detected OTA sensitively was designed, which used the 3D DNA walker by endonuclease Nb.BbvCI
powered and the nanozyme of porous Co3O4 nanodisks loaded Pt nanoparticles (Pt NPs@Co3O4) as a cascaded signal amplifying device. With OTA as a model target, the swing-arm probe of DNA walker was triggered. Next, probe on the magnetic beads was cleaved
and released by Nb.BbvCI to realize the first signal amplification. After that, the partial sequence of the released probe is well assembled with triple-strand DNA by strand reaction and the other sequence exposed as a capture probe was combined with the Pt NPs@Co3O4.
It can efficiently make the decomposition of H2O2, bring about significant current, and the signal was amplified again. The designed electrochemical aptasensor demonstrated outstanding detection linearity in the OTA concentration range of 1 pg/mL to 100 ng/mL, with a
detection limit of 0.194 pg/mL (S/N = 3), with excellent reproducibility and high specificity. It can be used to find OTA in food. This research supplies a potential split-new avenue for the sensitive detection of electrochemical aptasensor.
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