APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Xiao Xiao,Guowei Shan,Hong Gao,Zhe Ling,Yujia Tong,Xiaohui Ju,Weixing Li
Heavy metal ions (HMI), a non-degradable toxic substance, has raised widespread concerns in water purification. Membrane separation is a considerable and promising technology for removing HMIs from water, due to its environmentally friendly, clean and highly effective. This study reported a functional composite ultrafiltration membrane prepared from natural biomolecules ε-Poly-L-lysine hydrochloride (PLL), sodium lingo sulfonate (SLS) or sodium alginate (SA) based on the combination strategy of chemical graft and layer-by-layer assembly. The fabricated membrane exhibited a high pure water flux (2957 L·m −2 ·h −1 ), high capture efficiency for Cu 2+ (99.1 %) and Pb 2+ (98.9 %). Notably, the layer-by-layer assembly strategy provides abundant groups for HMI binding, ensuring the high capture efficiency. The fabricated membrane demonstrated a low flux decline (9 %), along with a high flux recovery ratio (99.2 %). Moreover, it exhibited exceptional stability and reusability across a wide pH range (pH 5–9), meanwhile maintaining a capture efficiency of approximately 99 % under near-neutral condition after undergoing 5 cycle experiments for both Cu 2+ and Pb 2+ . Our work builds a straightforward approach for UF membrane fabricating with excellent HMI capture capacity, displaying the potential application in HMI waste water purification.
研究领域:
环境科学
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材料科学
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化学工程
·
水处理
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产品关联
DOI
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Xudong Xu,Shuai Yin,Xinyu Zhai,Zide Wu,Jinlong Wang,Jiangfeng Ma,Xiaotian Peng,Hao Peng
Nano-encapsulated phase change materials (NEPCMs) with appropriate phase change temperature range and excellent anti-permeability have significant application value in the field of thermal regulation dressings. In this study, NEPCMs were synthesized using the microemulsion polymerization method, with eutectic fatty acids (LA-SA) as the core material and poly(methyl methacrylate) (PMMA) as the shell material. Subsequently, using the electrostatic adsorption method, chitosan (CS) was successfully coated onto the outer surface of NEPCMs, resulting in the preparation of a novel bilayer shell nano-encapsulated phase change materials (BS-NEPCMs) for thermal regulation dressings. The impact of core/shell ratio, CS acid solution concentration and cross-linking agent content on capsule properties was investigated. It was found that BS-NEPCMs exhibited excellent cyclical stability and thermal stability. In particular, the CS shell layer provided excellent interfacial impermeability effects for the capsules, achieving an anti-permeability efficiency of 96.21 %. This not only helps address the problem of high leakage rates in single wall capsules, but also ensures that the capsules maintain high phase change enthalpies of 99.32 J/g and 96.38 J/g, respectively. The dressings based on BS-NEPCMs demonstrated good thermal regulation at 33–42 °C with maximum phase change enthalpies of 39.53 J/g and 34.08 J/g, respectively. Swelling and rheological analysis showed that the dressing exhibits a gel state, with a swelling ratio of 343 %, an elastic modulus of 15 kPa and a viscous modulus of 4 kPa. This study indicated that BS-NEPCMs hold significant promise for various applications in energy storage and body thermal regulation fields.
研究领域:
材料科学
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纳米技术
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热管理
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生物医学工程
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产品关联
DOI
高影响因子
Nano Research
·
2018
·Vol. 11
·期 8
·4245-4264
·
IF 7.994
Aouameur Djamila,Cheng Hao,Opoku-Damoah Yaw,Sun Bo,Dong Qiuling,Han Yue,Zhou Jianping,Ding Yang
Engineered stimuli-responsive drug delivery devices hold vast promise in biological applications for disease treatment due to their maximized therapeutic efficacy. In this study, a novel, stably cross-linked, and pH-sensitive biodegradable gel-micelle was constructed with amphiphilic conjugates of trimethylene dipiperidine-methacrylic anhydride-hyaluronic acid-stearylamine (TMDP-MA-HA-SA, TMHS) to improve tumor-targeting with flexible intracellular delivery of paclitaxel (PTX).The cross-linked methacrylate bonds significantly improved the biostability of TMHS gel-micelle (~ 200 nm) over the non-cross-linked under physiological conditions, while hyaluronic acid plays an important role in active tumor targetability. The gradual degradation of cross-linked hyaluronic acid shell was triggered by the concentrated hyaluronidase. Meanwhile, under acidic conditions (pH < 6.5), the tertiary amines of pH-sensitive TMDP moieties were protonated and thereby solubilized the gel-micellar core-portions. The resultant pH-triggered inner-core spaces rapidly prompted PTX release in the presence of multiple cytosolic enzymes that mainly degraded the remaining hydrophobic stearylamine core. During the in vitro cytotoxicity assay, PTX-loaded TMHS gel-micelles (CLTMHSPTX) revealed anticancer efficacy against human hepatocellular carcinoma HepG2 cells with IC 50 of 1.42 μg/mL (PTX concentration), significantly lower than other groups. In parallel, the in vivo anti-tumor efficacy of CL TMHS PTX gel-micelles against BALB/c xenograft tumor animal model demonstrated the greater tumor growth inhibition capacity of 72.06%, compared to other treatment groups at a safe concentration. Consequently, the cross-linked and stimuli-responsive CL TMHS PTX gel-micelles hold a great potential for flexible modulation of intracellular delivery of hydrophobic anticancer drugs with maximized antitumor efficacy.
研究领域:
药学
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生物医学工程
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癌症研究
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产品关联
DOI
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2021
·Vol. 21
·期 11
·2100189
·
IF 4.979
Xiao-Pei Li,Lin Zou,Oluwatosin David Abodunrin,Xiao-Wei Wang,Ning-Ping Huang
Three-dimensional (3D) cell culture using hydrogel scaffolds can closely resemble the natural extracellular matrix (ECM), which offers appropriate mechanical support for cells and regulates cellular behavior. In this study, a bacterial transpeptidase sortase A (SA) is used to prepare enzymatically cross-linked methacrylated hyaluronic acid (HA) peptides (HAMA-P) hydrogel, which reveals fast gel kinetics under high SA cross-linking concentrations and can be used as an injection hydrogel for tissue repair or extrusive 3D bioprinting. Furthermore, methacrylated gelatin (GelMA) is introduced to build the hybrid hydrogel (HAMA-P-GelMA) with double cross-linking of enzyme-UV, which has shown proper physical properties (mechanical properties, swelling, degradation rate, etc.) of the hydrogel matrix, and displayed desirable effects on cell viability, adhesion, and cell spreading, when compared to GelMA or HAMA-P single-network hydrogels. The HAMA-P-GelMA hybrid hydrogels provide a favorable 3D milieu for cell growth and can be used as a 3D bio-ink or a carrier of stem cells/cytokines for injectable tissue repair and filling.
研究领域:
生物医学工程
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组织工程
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材料科学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2023
·Vol. 11
·期 12
·2745-2753
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IF 7
Hang Zhao,Liang Zhang,Jinsuo Lu,Shouning Chai,Jinliu Wei,Yuning Yu,Ruoyan Miao,Lvling Zhong
Bacterial infections caused by the growth and reproduction of pathogenic bacteria on wounds are one of the main reasons that hinder wound healing. Antibacterial wound dressings protect wounds from bacterial infections. Herein, we developed a polymeric antibacterial composite film using polyvinyl alcohol (PVA) and sodium alginate (SA) as the substrate. The film used praseodymium-doped yttrium orthosilicate (Y2SiO5: Pr3+, YSO-Pr) to convert visible light into short-wavelength ultraviolet light (UVC) to kill bacteria. The YSO-Pr/PVA/SA showed upconversion luminescence in photoluminescence spectrometry tests, and the emitted UVC inhibited Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli and Pseudomonas aeruginosa) bacteria in antibacterial tests. In vivo animal tests showed that YSO-Pr/PVA/SA is effective and safe for inhibiting bacteria in real wounds. The in vitro cytotoxicity test further confirmed the good biocompatibility of the antibacterial film. In addition, YSO-Pr/PVA/SA exhibited sufficient tensile strength. Overall, this study demonstrates the potential of upconversion materials for use in medical dressings.
研究领域:
材料科学
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生物医学工程
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伤口护理
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抗菌技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Shuai Liu,Hao Qu,Lili Yao,Yu Mao,Ling Yan,Baolei Dong,Lei Zheng
Rapid and accurate nitrite ions (NO 2 − ) detection is necessary to ensure food safety. Herein, a new portable method for detecting NO 2 − using hydrogel microspheres is proposed. Specifically, N-(1-naphthyl) ethylenediamine (NED) was immobilized on sodium alginate (SA) to obtain the SA-NED conjugate via covalent linking. The SA-NED microspheres were prepared in a Ca 2+ solution using an extrusion strategy based on the strong chelation between SA and Ca 2+ . The SA-NED microspheres exhibited superior adsorption capacity for NO 2 − , which agrees with the Langmuir isotherm model. Then, based on the Griess reaction , a microsphere-based colorimetric sensor was developed for rapid, quantitative, and on-site NO 2 − detection via a smartphone-based sensing platform. The entire detection procedure can be completed within 35 min. The microsphere-based sensor exhibited a detection limit of 0.02 μg mL –1 with recoveries ranging from 89.7% to 104.8% in different food samples. Moreover, the microsphere-based sensor showed good tolerance to the complex food matrix. Water and milk samples could be directly measured without pretreatment , and pickles and sausages only required simple homogenization before measurements. Our study provides an effective approach for rapid and on-site monitoring of NO 2 − .
研究领域:
食品科学
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分析化学
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传感器与执行器
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产品关联
DOI
高影响因子
Acta Biomaterialia
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2023
·
IF 9.7
Yulin Jiang,Juehan Wang,Dan Sun,Zheng Liu,Lin Qi,Meixuan Du,Jing Wang,Yubao Li,Ce Zhu,Yong Huang,Yueming Song,Limin Liu,Ganjun Feng,Li Zhang
The strategies for modulating the local inflammatory microenvironment to inhibit intervertebral disc degeneration (IVDD) have garnered significant interest in recent years. In this study, we developed a "self-contained" injectable hydrogel capable of storing Mg 2+ while carrying nucleus pulposus (NP) cells, with the aim of inhibiting IVDD through immunoregulation . The hydrogel consists of sodium alginate (SA), poly(N-isopropylacrylamide) (PNIPAAm), silicate ceramics (SC), and NP cells. When injected into the NP site, PNIPAAm gelates instantly under body temperature, forming an interpenetrating network (IPN) hydrogel with SA. Ca 2+ released from the SC can crosslink the SA in situ , forming a SA/PNIPAAm hydrogel with an interpenetrating network (IPN) encapsulating the NP cells. Moreover, inside the hydrogel, Mg 2+ released from SC are effectively encapsulated and maintained at a desirable concentration. These Mg 2+ facilitates the local cell matrix synthesis and promotes immunomodulation (upregulating M2 / downregulating M1 macrophage polarization), thus inhibiting the IVDD progression. The proposed hydrogel has biocompatibility and is shown to enhance the expression of collagen II (COL II) and aggrecan . The potential of the injectable hydrogel in IVD repair has also been successfully demonstrated by in vivo studies. Statement of significance • A unique injectable hydrogel consisting of sodium alginate (SA), poly(N-isopropylacrylamide) (PNIPAAm), silicate ceramics (SC) and nucleus pulposus cells, has been created for intervertebral disc repair applications. • This is the first “self-contained” cell delivery system capable of releasing and subsequently storing Mg 2+ in the hydrogel reservoir for localized treatment. • The role of Mg 2+ in regulating the macrophage polarity transformation (from pro-inflammatory M1 to anti-inflammatory M2 phenotype) has been unveiled in the context of intervertebral disc repair.
研究领域:
生物材料
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组织工程
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再生医学
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生物医学工程
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骨科
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Yanting Feng,Zewei Luo,Juan Wu,Zhijun Huang,Lu He,Yaqiang Feng,Xian Li,Xudong Zhang,Yonghui Tian,Yongxin Li,Yixiang Duan
Present DNA logic platforms mainly focused on distinguishing a specific kind of cancer cell from several other types of cell lines, while limited efforts have been paid to high-throughput recognition of cancer cells. A new DNA logic platform coupled with multivariate statistical analysis was developed to classify seven kinds of cancer cells. Four confined DNA circuits containing localized catalytic hairpin assembly (LCHA) and apurinic/apyrimidinic endonuclease 1 (APE1)-assisted circuits were conjugated to a protein of streptavidin (SA) for DNA logic platform. The mismatch minimizes the circuit leakage of the confined DNA circuits by inhibiting the incorrectly intramolecular structure switch. Benefitting from the spatial-confined effect, the DNA logic platform as a modified AND logic gate sensitively responded to the dual intracellular biomarkers, thereby generating strong dual fluorescent output signals in living cells. Based on the fluorescent cell imaging, multidimensional data collected from 50 individual cells were concatenated as a dataset for multivariate statistical analysis. Principal component analysis (PCA) shows distinct clustering among the seven kinds of cancer cell lines. A random forest algorithm obtained the specificity and sensitivity of 100%. Therefore, the DNA logic platform coupling with multivariate statistical analysis shows promising potential for cancer diagnosis and treatment.
研究领域:
生物技术
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癌症生物学
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分子生物学
·
生物信息学
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DOI
高影响因子
Acta Biomaterialia
·
2025
·
IF 9.6
Yuanqi Chen,Kezheng Lei,Yinxi Li,Zhixiang Mu,Tengda Chu,Jiajun Hu,Bairui Zeng,Yi Wang,Jianliang Shen,Xiaojun Cai,Tianpeng Shi,Hui Deng
Oral mucosal wounds are more prone to inflammation due to direct exposure to various microorganisms. This can result in pain, delayed healing, and other complications, affecting patients' daily activities such as eating and speaking. Consequently, the overall quality of life for patients is significantly reduced. To address these challenges, we developed a multifunctional therapeutic nanoplatform, DATS@Arg-EA-SA, through the self-assembly of guanidinated dendritic peptides (Arg-EA-SA) that encapsulate diallyl trisulfide (DATS), a hydrogen sulfide (H 2 S) donor. The guanidine-rich surface of DATS@Arg-EA-SA efficiently neutralizes reactive oxygen species (ROS) in the ulcer microenvironment, generating nitric oxide (NO), which acts as the primary antimicrobial agent by disrupting bacterial membranes. Concurrently, the presence of glutathione triggers the release of H 2 S from DATS, providing supplementary antibacterial support. DATS@Arg-EA-SA effectively kills all bacteria, achieving results comparable to those of penicillin, a classical antibiotic. Moreover, it demonstrates superior sterilization efficacy against drug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), significantly outperforming penicillin. Following the initial antimicrobial phase, the nanoplatform transitions into an anti-inflammatory stage. H 2 S, in synergy with NO, facilitates the conversion of M1 macrophages to M2 macrophages, thereby reducing the expression of inflammatory factors. Importantly, the combination of H 2 S and NO provides effective analgesia by downregulating the expression of TRPV1 and TRPV4, thus restoring normal dietary behaviors and improving the overall quality of life. This system ultimately promotes collagen fiber deposition and accelerates the re-epithelialization of the ulcer wound, positioning DATS@Arg-EA-SA as a promising gas-delivery nanoplatform for rapid wound repair in the clinical treatment. Statement of Significance Oral mucosal wounds are highly susceptible to microbial infections, leading to inflammation, pain, delayed healing, and a significant decline in quality of life. We developed a multifunctional therapeutic nanoplatform (DATS@Arg-EA-SA) via the self-assembly of guanidinated dendritic peptides encapsulating the H 2 S donor DATS, which exhibited antibacterial, anti-inflammatory, and analgesic properties. In the oral ulcer microenvironment, DATS@Arg-EA-SA generates substantial NO under elevated ROS levels, while glutathione triggers the controlled release of H 2 S. NO disrupts bacterial membranes as the primary antibacterial agent, with H 2 S providing synergistic antibacterial effects. Furthermore, H 2 S and NO synergistically promote the transformation of M1 to M2 macrophages, attenuating inflammation. Importantly, the combined action of H 2 S and NO alleviates pain by downregulating TRPV1 and TRPV4, supporting the restoration of normal dietary behavior and improving quality of life.
研究领域:
药学
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纳米技术
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生物材料
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医学
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牙科
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炎症研究
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伤口愈合
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2018
·
IF 2.831
Xin Liu,Dan Wang,Jinshen Chu,Yang Xu,Wenjun Wang
Prostate-Specific Antigen (PSA) is a crucial biomarker for screening prostate cancer, but a sensitive and selective immunosensor for rapid quantification of serum PSA remains to be developed. In this study, a sandwich pair of nanobodies (Nbs) (i.e., Nb2 and Nb40) against PSA surface antigen was obtained from an alpaca-derived immune phage display library. A sandwich-type immunosensor for the sensitive and selective detection of PSA in serum samples was ingeniously designed based on the pair of Nbs. The small size of Nb40 allowed high capture densities on the surface of reduced graphene oxide (rGO) nanocomposed with massive Au nanoparticles ( [email protected] ), which significantly improved the conductivity and provided a large area to anchor many primary antibodies. The secondary antibody Nb2 fused with streptavidin -binding peptide (SBP) cooperated with Nb40 for PSA sandwiching. Accompanying introduction of horseradish peroxidase-streptavidin (HRP-SA) coupled with Nb2-SBP, the faradaic current was linearly correlated with the logarithm of PSA concentration in a range of 0.1–100 ng mL −1 . More importantly, this immunosensor exhibited excellent selectivity, stability, and reproducibility due to the sandwich pair Nbs. The proposed immunosensor was successfully applied in determining PSA in serum samples and could be used for the sensitive and specific detection of PSA.
研究领域:
生物技术
·
纳米技术
·
免疫学
·
癌症研究
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2022
·
IF 0
Yufeng Wang,Chen Ling,Jialin Chen,Haoyang Liu,Qingyun Mo,Wei Zhang,Qingqiang Yao
The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge. To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification. The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration. For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix. The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs. Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model. This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
研究领域:
组织工程
·
生物材料
·
再生医学
·
干细胞生物学
·
生物医学工程
✓ 已验证
DOI
PubMed
Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Yaowen Liu,Xiaorong Dong,Kaiwen Bao,Zhiyong Deng,Md Alomgir Hossen,Jianwu Dai,Wen Qin,KangJu Lee
In this study, potassium persulfate, N,N′-methylene bisacrylamide (MBA), and soluble starch (ST) were in situ cross-linked into porous antimicrobial starch particles containing N-halamine functional groups (PST–MBA–Cl). Then, polyacrylonitrile (PAN)/silica aerogel (SA)/zinc oxide (ZnO) beaded fibers containing PST–MBA–Cl particles were prepared by electrospinning. Scanning electron microscopy results demonstrated the uniform dispersion of the PST–MBA–Cl particles and ZnO on the surface and interior of the beaded fiber when the concentration of the PST–MBA–Cl particles was 15%. The composite beaded fibers prepared after chlorination and soaking in zinc salt solutions of different pH values had a higher degree of interior fluffiness. For the analysis of the effectivity of the prepared particles using 10 8 CFU/mL gram-positive ( S. aureus ) and gram-negative ( E. coli ) bacterial human pathogens , the inhibition zone was 15.97 ± 0.12 and 17.81 ± 0.23 mm, respectively. Meanwhile, the degradation efficiency of PAN and SA for the cationic dye methylene blue (MB) was 88.00% ± 1.83%. The PAN/SA/PST–MBA–Cl/ZnO composite beaded fiber showed good antibacterial properties and a fast recovery rate, and its degradation efficiency for MB was retained at ~ 80% after five cycles. Thus, the PAN/SA/ PST–MBA–Cl/ZnO beaded fibers can be used as an environment-friendly and reusable adsorbing material for the removal of dyes from industrial wastewater .
研究领域:
环境科学
·
材料科学
·
化学工程
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）