RSC Advances
·
2019
·Vol. 9
·期 6
·2877-2884
·
IF 3.049
Yanlin Li,Sen Yang,Xuegang Lu,Wenyuan Duan,Toshihiro Moriga
Fe3O4–Ag Janus composites were synthesized using a two-step solvothermal method. The optimal growth process was determined by investigating the relationship between the particle morphologies and reaction time. Magnetic and Raman spectroscopic measurements showed that the as-synthesized Janus composites have both good magnetic response and significant surface-enhanced Raman scattering (SERS) effects, as well as reproducibility. The calculated Raman enhancement factor reached an unprecedented magnitude of 109 compared with the values of other Fe3O4–Ag compounds. Furthermore, the SERS effect was exhibited even at a concentration of probe molecules as low as 10−13 M. This demonstrates that the as-synthesized Fe3O4–Ag Janus composite particles have promise for application as separable, highly sensitive SERS substrates.
研究领域:
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2023
·Vol. 15
·期 18
·8424-8431
·
IF 6.7
Bingfang Zou,Shiyun Lou,Jie Duan,Shaomin Zhou,Yongqiang Wang
Magnetic-based microfluidic SERS biosensors hold great potential in various biological analyses due to their integrated advantages including easy manipulation, miniaturization and ultrasensitivity. However, it remains challenging to collect reliable SERS nanoprobe signals for quantitative analysis due to the irregular aggregation of magnetic carriers in a microfluidic chamber. Here, magnetic/plasmonic hybrid nanostirrers embedded with a Raman reporter are developed as capture carriers to improve the reliability of microfluidic SERS biosensors. Experimental results revealed that SERS signals from magnetic hybrid nanostirrers could serve as microenvironment beacons of their irregular aggregation, and a signal filtering method was proposed through exploring the relationship between the intensity range of beacons and the signal reproducibility of SERS nanoprobes using interleukin 6 as a model target analyte. Using the signal filtering method, reliable SERS nanoprobe signals with high reproducibility could be picked out from similar microenvironments according to their beacon intensity, and then the influence of irregular aggregation of magnetic carriers on the SERS nanoprobe could be eliminated. The filtered SERS nanoprobe signals also exhibited excellent repeatability from independent tests, which lay a solid foundation for a reliable working curve and subsequent accurate bioassay. This study provides a simple but promising route for reliable microfluidic SERS biosensors, which will further promote their practical application in biological analysis.
研究领域:
分析化学
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生物医学工程
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 11
·3649-3658
·
IF 4.019
Qianqian Fang,Yingying Li,Xinxing Miao,Yiqiu Zhang,Jun Yan,Tainrong Yu,Jian Liu
Serious healthcare concerns have been raised on the issue of antibiotic residues after overuse, especially by accumulation in the human body through food webs. Here, we report a methodological development for sensitive detection of antibiotics with aptamer conformation cooperated enzyme-assisted SERS (ACCESS) technology. We design and integrate a set of nucleic acid oligos, realizing specific recognition of chloramphenicol (CAP) and efficient exonuclease III-assisted DNA amplification. It features a “signal-on” analysis of CAP with the limit of detection (15 fM), the lowest concentration detectable in the literature. Our method exhibits a high selectivity on the target analyte, free of interference of other potential antibiotic contaminants. The ACCESS assay promises an ultrasensitive and specific detection tool for trace amounts of antibiotic residues in samples of our daily life.
研究领域:
分析化学
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生物技术
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食品安全
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环境科学
✓ 已验证
DOI
PubMed
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 11
·988
·
IF 5.743
Linmin Chen,Meihuang Zeng,Jingwen Jin,Qiuhong Yao,Tingxiu Ye,Longjie You,Xi Chen,Xiaomei Chen,Zhiyong Guo
Nanoenzyme reactors based on shell-isolated colloidal plasmonic nanomaterials are well-established and widely applied in catalysis and surface-enhanced Raman scattering (SERS) sensing. In this study, a “double wing with one body” strategy was developed to establish a reduced food antiseptic sensing method using shell-isolated colloidal plasmonic nanomaterials. Gold nano particles (Au NPs) were used to synthesize the colloidal plasmonic nanomaterials, which was achieved by attaching ferrous ions (Fe2+), ferric ions (Fe3+), nitroso (NO−) group, cyanogen (CN−) group, and dopamine (DA) via coordinative interactions. The oxidation-induced reaction was utilized to generate •OH following the Fe2+-mediated Fenton reaction with the shell-isolated colloidal plasmonic nanomaterials. The •OH generated in the cascade reactor had a high oxidative capacity toward acid preservatives. Importantly, with the introduction of the signal molecule DA, the cascade reactor exhibited also induced a Raman signal change by reaction with the oxidation product (malondialdehyde) which improved the sensitivity of the analysis. In addition, the stable shell-isolated structure was effective in realizing a reproducible and quantitative SERS analysis method, which overcomes previous limitations and could extend the use of nanoenzymes to various complex sensing applications.
研究领域:
分析化学
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纳米生物学
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食品科学
✓ 已验证
DOI
高影响因子
Plasmonics
·
2023
·
IF 3
Dong Jun,Li Chenlu,Wang Yan,Cao Yi,Fan Yimeng,Han Qingyan,Gao Wei,Wang Yongkai,Qi Jianxia
Surface-enhanced Raman scattering (SERS) is widely employed because it offers quick, microscopic, and traceless detection. This study used high-voltage and low-voltage ultrasonic oscillation to embed gold-silver nanoparticles (Au-Ag NPs) into the pores of chemically modified ultrathin anodic alumina (AAO) films, resulting in a highly sensitive three-dimensional SERS substrate. We improved the substrate’s stability and Raman activity by adjusting the particle alloy ratio. For the substrate in this alloy ratio, the Raman signal of probe molecules (Rh6G) adsorbed on the substrate surface is enhanced. the enhancement factor (EF) was as high as 1.40 × 10 7 , the relative standard deviation (RSD) of 10.6%, and the concentration of Rh6G shows a linear relationship with Raman intensity, with a linear correlation coefficient of 0.961. In addition, we evaluated the substrate’s detection effect on thiram molecules. It has been proven that this structure has good practicality and high sensitivity as a Raman enhanced substrate.
研究领域:
纳米技术
·
材料科学
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分析化学
·
表面科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Zhuxu Xu,Adelakun Moses Erinomo,Zhenhua Dan,Fengxiang Qin,Hui Chang
Accurate and sensitive surface-enhanced Raman scattering (SERS) substrates are urgently demanded for the various analytic techniques. The SERS substrates with multimodal and gradient porous structure, utilized for the Rhodamine 6G (R6G) detection, have been designed and successfully fabricated from the thermal diffusion couples of Al and Cu foils via chemical dealloying. The structural inheritance of the initial microstructure of thermal diffusion couple precursors governs the formation of the final layer-by-layer gradient porous Cu with different characteristic pore sizes and morphology. An outmost surface layer of disordered lipstick-like porous structure on the SERS substrates enhances SERS effects as has been expected. The SERS activity of R6G Raman probes on gradient porous Cu is evaluated as a SERS enhancement factor of 6.63 × 10 12 and limit of detection of 2.10 × 10 −17 mol/L, higher than other porous SERS substrates. Meanwhile, the present SERS substrates are flexible and exhibit good bending properties due to the unreacted Cu layer as a supporting skeleton and other gradient porous Cu layers. The superior SERS performances are considered to result from the synergetic effects of the surface scattering of porous Cu rods and enhancements of the high electromagnetic fields between the small gaps between the nanopores.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Dongjian Li,Mingxin Han,Liangzhuang Wang,Chengyun Si,Yingxiang Du,Xuefang Luo
Pesticide residues pose serious threats to food safety and human health, highlighting the need for rapid and sensitive detection methods. Traditional techniques are often time-consuming and complex, whereas Surface-Enhanced Raman Spectroscopy (SERS) offers a fast and highly sensitive alternative. In this study, silver nanoparticles (AgNPs) were synthesized via a one-pot hydrothermal method using trisodium citrate (TSC) as a reducing agent and polyvinylpyrrolidone (PVP) as a capping agent. By optimizing PVP and TSC concentrations, a novel AgNPs@capillary SERS substrate was fabricated through in situ assembly of AgNPs onto the inner walls of glass capillaries. The substrate enabled rapid, on-site detection of pesticide residues, specifically the neonicotinoid acetamiprid (ACE) and benzimidazole fungicide carbendazim (CBZ). SERS measurements showed strong linear correlations for ACE (R² = 0.9713, 0.01–0.5 μg/mL) and CBZ (R² = 0.9566, 0.005–0.5 μg/mL). Importantly, the detection limits reached 2.28 × 10⁻³ μg/mL for ACE and 6.25 × 10⁻³ μg/mL for CBZ, demonstrating excellent sensitivity. This study confirms the effectiveness of the one-pot synthesized AgNPs@capillary SERS platform as a practical tool for trace-level pesticide monitoring in environmental and food safety contexts.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Guohua Qi,Dan Sun,Yu Tian,Chen Xu,Ying Zhang,Dandan Wang,Kongshuo Ma,Shuping Xu,Yongdong Jin
Caspase-3 is considered as one of the key proteases that can spontaneously regulate the life activities of cells, and its activation (usually is a slow process) will execute the apoptosis process of cells. Rapid activation of caspase-3 on demand in living-cells is therefore highly desired toward precise cancer therapy but it is still a key challenge. Herein, we applied electrostimulus (ES) to achieve fast activation of caspase-3 and trigger cell apoptosis, and developed a smart magnetic-plasmonic assembly nanoprobes (A-nanoprobes) to real-time trace cellular caspase-3 activation at the single cell level. The designer core–satellite A-nanoprobe, working specific to the activated caspase-3 via a disassembly tactic, provides strong “hot spots” to improve the sensitivity and therefore enables SERS sensing of cellular caspase-3 upon activated by ES. Single-cell analysis revealed that the ES can rapidly activate the apoptosis pathway of caspase-3 on demand to make the DNA fragmentation and ultimately induce the cell apoptosis. Such method and nanoplatform were further used to monitor ES-triggered caspase-3 activation in cell apoptosis process of different cell types, revealing that more caspase-3 will be activated for cancerous cells than normal cells during the ES to induce cells apoptosis. This strategy and platform are promising for detecting cellular caspase-3 and other enzymes in the process of cancer diagnosis and treatments.
研究领域:
生物医学工程
·
纳米技术
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癌症研究
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 24
·5670-5679
·
IF 5.227
Ziyan Song,Siyu Chen,Qiushi He,Hu Liang,Guangyao Huang,Pan Li,Zongyao Hao,Liangbao Yang
The rapid and sensitive detection of ultra-trace marker molecules from biological samples is of great significance for the wide application of surface-enhanced Raman spectroscopy (SERS) methods in clinical diagnosis and disease monitoring. However, the cumbersome biological sample processing procedures and the poor enrichment of target analytes in hot spots hinder the practical applications of SERS methods. In this paper, we synthesized a novel floating SERS substrate by a simple one-step oxidation process, annealing and in situ chemical etching to form Ag-NPs@Cu-NW bundles on copper mesh (CM). In particular, under spontaneous bottom-up capillary action, the pressure difference at different nanogaps drives uric acid molecules to actively enter hot spots, so that the Ag-NPs@Cu-NW bundle nanostructure with the advantages of a light weight CM is capable of preventing the common coffee-ring effect and enhancing the spatial enrichment of analytes. Therefore, this SERS substrate realizes highly sensitive detection of uric acid at a level of 50 nM in pretreatment-free urine. Currently, this portable, flexible, simple, fast and cost-effective SERS substrate has great potential for early screening and clinical diagnosis of diseases in different biofluids.
研究领域:
分析化学
·
生物医学工程
·
临床诊断
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jinglei Yang,Bohan Zhang,Qingling Nie,Ruping Zheng,Ping Lin,Chuyi Wang,Yudong Lu,Yunli Xu,Ruiyun You
Uric acid, urea, and other metabolites in urine after exercise often reflect chronic injury syndrome in athletes. However, traditional urine detection methods have issues such as high costs and low detection sensitivity. SERS can rapidly, continuously, and sensitively monitor metabolites in human urine. In this research, a combined SERS substrate (CMBCM@Ag NPs@PGA) was developed based on the carboxymethyl modification of the bacterial cellulose membrane (BCM) surface. The numerous carboxyl groups on the CMBCM surface made it easier for silver ions to be adsorbed, leading to their conversion into silver nanoparticles (Ag NPs) when a reducing agent was introduced. This process allowed the nanoparticles to firmly adhere to the CMBCM surface, forming a uniform and stable “hot spot.” “The CMBCM@Ag NPs@PGA substrate maintains excellent stability and sensitivity in the assay.” It can detect very small amounts of urea and uric acid in urine with high sensitivity, with LOD of 1.05 μM for urea and 0.0075 μM for uric acid. Additionally, it exhibits good stability, antibacterial properties, and cell compatibility. In addition, the substrate can be used as a sensor to monitor pH in real-time. This expands the use of cellulose in flexible SERS sensing and detecting human exercise metabolic health.
研究领域:
生物医学工程
·
分析化学
·
运动医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiahao Zhang,Lei Xu,Yue Hu,Li Sun,Yujiao Xie,Xinyu Miao,Aochi Liu,Zhiwei Hou,Yixing Gou,Aiguo Wu,Jie Lin
Gastric cancer (GC) is a disease with high mortality rates and remains a central focus in medical research. Efficient enrichment, separation, and precise diagnosis of human gastric cancer (HGC) cells from biological samples are essential for early detection and treatment. However, the similarity in size of white blood cells (WBCs) and HGC cells in gastric fluid has posed vital challenges in the rapid identification and precise diagnosis of GC. To address this issue, a smart system that combines enrichment and detection of HGC cells using inertial microfluidic chips and a label-free surface-enhanced Raman spectroscopy (SERS) bioprobe has been developed. The HGC cells in simulated gastric fluid and ascites are rapidly separated and enriched by a spiral microfluidic chip. Silver bioprobes with excellent SERS performance are prepared to collect the label-free SERS spectra of HGC cells, normal gastric cells, and WBCs. SERS spectral data analysis and model prediction are performed by machine-learning-assisted principal component analysis-linear discriminant analysis (PCA-LDA). The result shows that HGC cells can be well identified with an accuracy as high as 96%. The microfluidic-SERS system proposed in this study can effectively separate and accurately identify tumor cells in body fluids, providing a new method of precision gastric cancer detection.
研究领域:
医学
·
生物工程
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 25
·8879-8888
·
IF 7.367
Xiu Liang,Yi-Shuo Wang,Ting-Ting You,Xiao-Juan Zhang,Nan Yang,Guang-Sheng Wang,Peng-Gang Yin
Interfacial self-assembly of ordered nanostructures at oil–water interfaces towards the fabrication of nanofilms has attracted the interest of plenty of scientists, since its discovery in 2004. Herein, further developments have been achieved, and we report a new strategy for the synthesis of a three-dimensional (3D) hierarchical nanostructure, through an interfacial synthesis driven microemulsion process. Thus, the synthesis route has been simplified, with the rigorous experimental conditions of traditional compositing technology. Combined with a two-step seed-mediated growth method for preparing uniform Ag-NPs, a plasmonic 3D MoS2-NS@Ag-NP nanostructure was successfully developed as a Surface-Enhanced Raman Scattering (SERS) active substrate, with plenty of surface hot spots, leading to an enhancement factor (EF) of 1.2 × 108 derived from both electromagnetic mechanism (EM) and chemical mechanism (CM) effects. The 3D MoS2-NS@Ag-NP nanostructure can be applied to detect trace thiram in apple juice and local lake water, with a detection limit as low as 10 ppb (42 nM), which is much lower than the maximal residue limit (MRL) of 7 ppm in fruit prescribed by the U.S. Environmental Protection Agency (EPA). Furthermore, quantitative analysis was achieved in the range of 10 ppb–1 ppm with good homogeneity and selectivity.
研究领域:
材料科学
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）