BIOELECTROCHEMISTRY
·
2026
·
IF 4.9
Zhen Peng,Yihao Long,Zhihao Tu,Muhammad Tahir,Liang He
Nutrition competition exists in the tumor microenvironment (TME), which is considered as an important factor of T cell exhaustion. Various amino acids are the targets for both tumor cells and T cells, including L-Tyr, which plays an essential role in normal function of T cells. Herein, a novel sensing interface of MIP/Fc/PEDOT:PSS-PPy/graphite/Au established on a PCB substrate, was proposed for L-Tyr detection. This sensor was systematically characterized and showed excellent repeatability, stability, reproducibility, and specificity. Meanwhile, the sensor exhibited good detection capability, with a linear range of 1 nmol/L to 1 mmol/L, a limit of detection of 1.62 × 10 −11 mol/L, and a sensitivity of 97.14 μA/(mol/L). An in-vitro co-culture model of T cells and tumor cells was established to mimic the TME, and its culture medium was used as real samples for L-Tyr determination via the proposed sensor. The results demonstrated that the failure of L-Tyr competition by T cells in the TME was a vital cause of their exhaustion, which was further validated by the IL-2 expression of T cells within the TME using ELISA. Collectively, our study is expected to provide a new strategy for tumor treatment, by supplementing exogenous amino acids to alleviate T cell exhaustion.
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PubMed
高影响因子
Nano Today
·
2025
·
IF 10.9
Wenyan She,Pengdi Zhang,Linlin Guo,Yu Zhang,Haoyi Zhu,Yaping Wang,Yichao Zheng,Lin Hou
T cell-based immunotherapies benefit only a minority of patients, primarily due to insufficient CD8 + T cell quantity and functional exhaustion. However, integrated strategies addressing both challenges remain scarce. Epigenetic dysregulation has recently been recognized as an important factor interfering with T cell responses in the tumor microenvironment. Herein, we develop an epigenetic nano-editor (P@cPG/sg) that simultaneously targets the epigenetic lysine-specific demethylase 1 (LSD1) in both tumor cells and T cells to reprogram the intratumoral T cell niche. The nano-editor consists of two guanidinium-based cationic carriers complexed with LSD1-targeting CRISPR/Cas9 plasmids, and can de-crosslink into tumor cell-targeting nanoparticles (P M G/sg) and T cell-targeting nanoparticles (P T G/sg). P M G/sg downregulates LSD1 in tumor cells, leading to the upregulation of chemokines that promote CD8 + T cell infiltration. Meanwhile, P T G/sg reduces LSD1 in T cells, lowering PD-1 expression, significantly enhancing T cell proliferation and rejuvenating exhausted cytotoxic CD8 + T cells in situ . Simultaneous LSD1 knockdown establishes a self-reinforcing cascade of anti-tumor immunity, achieving over 75 % tumor growth inhibition in both murine and humanized gastric cancer models. Thus, this nano-editor represents a standalone epigenetic immunotherapy that synchronously enhances intratumoral CD8 + T cell quantity and effector function, overcoming key limitations of current T cell-based immunotherapies.
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高影响因子
NMR IN BIOMEDICINE
·
2025
·Vol. 38
·期 8
·e70093
·
IF 2.7
Zhongyichen Huang,Qiting Wu,Jing Zhang,Hao Tang,Gang Wu,Yao Zhang,Yi Wang,Dong Liang,Xiaoming Li,Yin Wu
Diabetes mellitus (DM) causes skeletal muscle damage. Energy metabolism alterations in diabetic muscles remain to be elucidated. Chemical exchange saturation transfer (CEST) MRI is increasingly adopted in imaging muscle energy metabolism. This study aimed to investigate energy metabolism alterations in diabetic muscles using CEST MRI. Six adult New Zealand rabbits with alloxan-induced DM were scanned on a 5 T MR imager before and 2, 4, 6, and 8 weeks after the DM model induction, with six normal rabbits as controls. T 2 -weighted imaging, B 1 field mapping, and CEST imaging under four B 1 amplitudes of 0.75, 1.0, 1.25, and 1.5 μT were performed, respectively. Blood glucose level was measured before each MRI experiment. Inverse magnetization transfer ratio analysis was adopted to quantify CEST effects of two energy metabolites of creatine (Cr) and phosphocreatine (PCr), from which their ratio was calculated. B 1 inhomogeneity was corrected using a smoothing spline interpolation algorithm. pH-sensitive CrCEST ratiometric was measured by dividing CrCEST under B 1 of 1.25 μT with that under 0.75 μT. Paired student's t -test and one-way ANOVA were conducted with p < 0.05 considered statistically significant. Blood glucose level measurements confirmed the successfully induced DM model. The controls showed no significant changes in Cr- and PCr-weighted CEST signals throughout the study. In contrast, a significant increase of Cr-weighted CEST signals started in 2 weeks ( p = 0.049) and became more pronounced in Week 6 ( p = 0.045) and Week 8 ( p = 0.002) after the DM model induction, whereas PCr-weighted CEST signals remained constant among multiple time points, leading to significantly reduced PCr/Cr in Week 6 ( p = 0.047) and Week 8 ( p = 0.011). Meanwhile, CrCEST ratio did not show significant alterations ( p > 0.05). The significantly increased Cr-weighted CEST and reduced PCr/Cr with time implied disrupted energy metabolism in diabetic muscles, providing supplementary information for better understanding of DM-related muscle impairments from the metabolic level.
研究领域:
生物医学工程
·
内分泌学
·
放射医学
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高影响因子
CRYSTENGCOMM
·
2018
·Vol. 20
·期 9
·1220-1231
·
IF 3.304
Jianhua Zhang,Qingshan Fu,Yongqiang Xue,Zixiang Cui
Trigonal selenium (t-Se) nanomaterials with different morphologies present distinct properties and great potential applications in electric devices. However, controlled synthesis of t-Se nanomaterials with various morphologies is difficult in a typical preparation process. Therefore, it is imperative to develop an easily controlled and high-efficiency method to prepare t-Se with various morphologies. Herein, a precursor conversion method was proposed to prepare t-Se nanomaterials with different morphologies. That is, uniform amorphous selenium (a-Se) nanospheres were prepared by reducing sodium selenite with glucose, and then t-Se nanomaterials with morphologies of spheres, tubes, rods, belts and wires were obtained by different subsequent treatments for the conversion of a-Se into t-Se. The results demonstrate that the t-Se nanospheres were obtained by hydrothermal treatment at 150 °C, t-Se nanorods and nanotubes by ultrasonication of a-Se in water and with the addition of PVP K30 for nanotubes, t-Se nanowires by the aging of a-Se in ethanol and in a dark environment, and t-Se nanobelts by increasing the concentration of a-Se in ethanol. The conversion processes from a-Se nanospheres into t-Se 1D nanostructures comply with a “solid–solution–solid” formation mechanism, while the conversion from a-Se nanospheres into t-Se nanospheres complies with the mechanism of crystalline phase transformation. The method provides us a mild and easily controlled route for the preparation of t-Se nanomaterials with desired morphologies.
研究领域:
纳米科学
·
材料化学
·
晶体生长
·
功能材料
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高影响因子
FUEL
·
2022
·
IF 8.035
Songpeng Cheng,Liangcheng An,Zhenling Li,Suli Liu,Junmin Lv,Subing Fan,Tiansheng Zhao
The products with higher carbon numbers from Fischer-Tropsch (F-T) synthesis are very complex and hard to directly be analyzed on GC due to their very high boiling point. In this work, the qualitative and quantitative analytical method for refined F-T wax samples with C 7 to C 38 was established on GC–MS. All components were well separated, qualified and quantified. The analytical errors were less than ±2 % and the errors of recovery experiments were less than ±5 %. Thereafter, this method was successfully applied in GC analysis with errors less than ±0.75 % and the analytical method on GC was also built, which will provide a more convenience way in analysis of refined F-T wax samples. Based on the obtained analytical conditions and the relative calibration factors of paraffins, the qualitative and quantitative method for the cracking products from refined F-T wax was further built according to a similar method by using simulated samples with the conversion from 5 % to 80 %. The thermal cracking of F-T wax 5# was performed and the content of LAOs in liquid mixtures reached over 50 % at 650 °C. This study simplifies the previous analysis methods which can only be completed by using a variety of standard methods and provide convenience for analysis of refined F-T wax from coal-to-liquid plant.
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高影响因子
Viruses-Basel
·
2022
·Vol. 14
·期 11
·2431
·
IF 5.818
Jing Zheng,Wenyuan Chen,Hao Xiao,Fan Yang,Xiaowu Li,Jingdong Song,Lingpeng Cheng,Hongrong Liu
GP4, a newRalstonia solanacearumphage, is a short-tailed phage. Few structures ofRalstonia solanacearumphages have been resolved to near-atomic resolution until now. Here, we present a 3.7 Å resolution structure of the GP4 head by cryo-electron microscopy (cryo-EM). The GP4 head contains 540 copies of major capsid protein (MCP) gp2 and 540 copies of cement protein (CP) gp1 arranged in an icosahedral shell with a triangulation number T = 9. The structures of gp2 and gp1 show a canonical HK97-like fold and an Ig-like fold, respectively. The trimeric CPs stick on the surface of the head along the quasi-threefold axis of the icosahedron generating a sandwiched three-layer electrostatic complementary potential, thereby enhancing the head stability. The assembly pattern of the GP4 head provides a platform for the further exploration of the interaction betweenRalstonia solanacearumand corresponding phages.Keywords:Ralstonia solanacearumphage;icosahedral head;triangulation number T = 9;trimeric CP;Cryo-EM
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高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Hankui Sang,Congming Tang,Kai Ma,Xinli Li
The scarcity of fresh water resources necessitates us to develop an efficient technique for sustainable production of clean water from seawater and wastewater. Here, we constructed the 1 T-MoS 2 /PDA (polyaniline) as a photothermal material for purification of seawater and wastewater to produce clean water through interfacial evaporation by virtue of sunlight. 1 T-MoS 2 has a higher evaporation capacity of water than 2 H-MoS 2 , which was selected to prepare 1 T-MoS 2 /PDA composite for photothermal conversion. As expected, 1 T-MoS 2 /PDA composite offers a higher evaporation rate of water than both 1 T-MoS 2 and PDA , which owes to an excellent light absorbance, low light reflectance and good wettability . Under irradiation of 1 sun, the evaporation rate of water over the 1 T-MoS 2 /PDA composite in pure water reached 1.51 kg m −2 h −1 , and the photothermal conversion efficiency reached 92%. Encouragingly, 1 T-MoS 2 /PDA composite can be well used to treat real seawater and various wastewater such as organic dye solution and heavy metal ion solution, in which the evaporation rate of water is close to pure water, and the quality of water is better than the standard of drinking water established by the World Health Organization (WHO). Besides, 1 T-MoS 2 /PDA composite still maintain an excellent evaporation performance after treatment of acidic solution and alkaline solution, indicating an excellent durability. This work provides a feasible solution for producing clean water by solar-driven interfacial evaporation from seawater and wastewater.
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高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2026
·
IF 5.1
Zengke Guo,Jian Deng,Lin Sheng,Guangsheng Luo
A buffer microzone based T-junction is designed for viscous droplets formation. • A non-monotonic effect of the dispersed phase flow rate on the droplet size is observed. • Inherent mechanisms of the viscosity-induced droplet size reduction are revealed. • Two dimensionless prediction models are developed for the viscous droplet size. Generating viscous droplets in microchannels remains a technical challenge. Here, a buffer microzone in T-junction microchannel was designed to balance the liquid–liquid two-phase interaction for the controllable preparation of viscous droplets. The polyvinylpyrrolidone (PVP) and chitosan aqueous solutions with a wide range of viscosity variation were employed as the dispersed phase. The results show that compared with the conventional T-junction microchannel, the modified T-junction effectively suppresses the long jetting regime and broadens the operation window of the squeezing and dripping regimes. The formed viscous droplet could be much smaller and the size distribution is much narrower. Importantly, the influence of the dispersed phase flow rate on the droplet size is revealed to be non-monotonic, depending strongly on both the continuous phase flow rate and the fluid viscosity. With the new finding, a droplet size prediction model incorporating the capillary number, fluid viscosity ratio, and flow rate ratio is proposed.
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高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2023
·
IF 10.8
Tiantian Liu,Zehang Zhou,Mengxing Zhang,Puxin Lang,Jing Li,Zhenmi Liu,Zhirong Zhang,Lin Li,Ling Zhang
The cuproptosis cell death pathway brings fresh opportunities for tumor therapy. However, efficient and targeted cuproptosis induction in tumors is still a challenge. Unfortunately, the well-known cuproptosis initiator, disulfiram and copper complex (DSF/Cu 2+ ), also increases PD-L1 level in tumors, which may diminish the final therapeutic outcome. In this study, DSF/Cu 2+ -loading MXene nanosheets are coated with PD-1 overexpressing T cell membrane to generate CuX-P system. CuX-P could recognize and stick to PD-L1 on tumor cells like a patch, which promotes the endocytosis of both CuX-P and PD-L1 by tumor cells. Following internalization and release of DSF/Cu 2+ in the cytoplasm, PD-L1 expression is upregulated. However, due to the presence of CuX-P in the tumor microenvironment , the then supplemented PD-L1 on tumor surface again binds CuX-P for internalization. This feedback loop keeps blocking and consuming the PD-L1 on tumor surface and promotes the enrichment of CuX-P in tumors to induce cuproptosis. After CuX-P treatment with laser irradiation, strong anti-tumor immune responses are stimulated in a mouse model with triple-negative breast cancer. Thus, this study develops a tumor-targeted biomimetic system that offers simultaneous cuproptosis killing, photothermal therapy (PTT) and immunotherapy in mice.
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高影响因子
ChemCatChem
·
2021
·Vol. 13
·期 13
·3184-3197
·
IF 5.686
Chao Huang,Can Zhu,Mingwei Zhang,Yongwu Lu,Qianhao Wang,Heming Qian,Jiangang Chen,Kegong Fang
Graphical Synergetic effect : The physically mixed CuCoAl|t-ZrO 2 multifunctional catalyst could achieve superior selectivity towards alcohols while simultaneously inhibiting the generation of hydrocarbons from CO hydrogenation. Selective synthesis of higher alcohols from syngas is promising but remains challenging owing to the low stability and selectivity towards higher alcohols. Here we introduce a CuCoAl|t-ZrO 2 (t denotes tetragonal crystal phase) multifunctional catalyst that can achieve an alcohol selectivity of 64.8 % with 79.6 % C 2+ OH, and a total selectivity of alcohols and olefins to 81.7 %. The proper proximity was tuned to increase selectivity towards alcohols and decrease the selectivity towards hydrocarbons. The lower surface [H*]/[C*] ratio due to the stronger basic sites of the CuCoAl|t-ZrO 2 limited alkyl (CH x ) species hydrogenation, and promoted CO insertion and carbon chain growth, resulting in improving the alcohols selectivity. Meanwhile, the weaker adsorption of bridge-type adsorbed CO and the lower decomposition rate of multi-bonded adsorbed CO over CuCoAl|t-ZrO 2 inhibited hydrocarbon generation. The selective promotion of the reaction route via the synergetic effect between CuCoAl and t-ZrO 2 was proposed to boost higher alcohols selectivity. This work may aid the rational design of effective multifunctional catalyst with synergistic effects for tuning selectivity.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yunfeng Sun,Shuang Li,Ruipeng Chen,Pian Wu,Jun Liang
The T-2 toxin is generated as a secondary metabolite by various species of Fusarium that are found extensively in barley, wheat, maize, and oats; consequently, the development of methods that can rapidly and efficiently detect this toxin is vital. Herein, we report on a novel and highly sensitive method based on DNA hydrogels and etched gold nanorods (AuNRs) to detect the T-2 toxin. The T-2 toxin aptamer acts as a cross-linking agent for a hydrogel in which horseradish peroxidase (HRP) is uniformly embedded. The target T-2 toxin binds to the aptamer, causing the hydrogel to collapse and release the embedded HRP, which catalyzes the reaction of H 2 O 2 with KI to produce I 2 that etches the AuNRs. The UV spectrum of the AuNRs is blue-shifted following etching; this shift is proportional to the T-2-toxin content. This method offers a low limit of detection (0.87 pg mL −1 ), low limit of quantification (2.90 pg mL −1 ), and wide detection range (0.01–10000 ng mL −1 ). Moreover, coffee, corn, and soybean were assayed using the optimized method. The approach offers good recoveries and relative standard deviations of 93.72–103.07 % and 3.87–7.07 %, respectively. The total assay time was 1.5 h. Thus, the new method is promising as an ultrasensitive assay for detecting various small molecules in complex matrices.
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高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2019
·
IF 3.372
Piao Liu,Jianxin Cao,Zheng Xu,Chunliang Yang,Xiaodan Wang,Fei Liu
Core-shell structured ZSM-5@t-ZrO 2 catalysts were prepared by a hydrothermal coating method, and their physicochemical properties, catalytic performance, and deactivation behavior in thiolation reactions were studied using a ZSM-5/t-ZrO 2 physically-blended catalyst as a comparison. Reactions using the core-shell ZSM-5@t-ZrO 2 catalyst were carried out at a pressure of 0.7 MPa, 370 °C, a H 2 S:CH 3 OH molar ratio of 2:1, and a N 2 flow rate of 90 ml·min −1 . The conversion of CH 3 OH, and the selectivity and yield of CH 3 SH under these conditions were 92.07%, 90.89%, and 83.68%, respectively, and the catalytic lifetime was longer than 30 h. Compared with the ZSM-5/t-ZrO 2 physically-blended catalyst, the CH 3 SH yield and the catalytic lifetime of the core-shell structured ZSM-5@t-ZrO 2 catalyst were higher by 5.37% and 150%, respectively. The unique reaction pathway of the shell (t-ZrO 2 , Lewis (L) base active site)-core (ZSM-5, Brǿnsted (B) acid active site) and the Lewis-Brønsted acid active center based on a composite solid-solution interface were mainly responsible for the high activity of the thiolation reaction. Meanwhile, the formation of a micro-mesoporous system increased the diffusion of molecules and reduced the deactivation rate of carbon- and sulfur-containing deposits. Generally, carbon- and sulfur-containing substances deposited on catalysts were mainly composed of polymethylbenzene, polymethyl naphthalene, cyclic elemental sulfur, and other compounds containing C S and S S bonds. Finally, the core-shell structured catalysts showed excellent resistance to deactivation by carbon- and sulfur-containing deposits.
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