LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Cunhui Wu,Zhiyang Qin,Yixin Liu,Xinguang Qin,Gang Liu,Xinlin Wei,Haizhi Zhang
Designing efficient nanozyme to construct rapid analytical method for tannic acid (TA) content control has significant influence in food science area. Herein, by one-step self-assembly between Fe 3+ and 2, 3, 6, 7, 10, 11-hexahydroxytriphenylene (HHTP), an amorphous nanomaterial (Fe-HHTP) was synthesized and fully characterized by TEM, AFM, XRD, EDS element mapping and XPS. Then, its peroxidase-like activity was explored by using H 2 O 2 and 3, 3′, 5, 5′- tetramethylbenzidine (TMB) as typical substrates. Due to the inhibition of TA on Fe-HHTP catalyzed chromogenic reaction, a rapid and accurate colorimetric method for TA detection was constructed. After thorough verification, the method featured with merits of low limit of quantitation (0.50 μmol/L), good linear range (0.5–100.0 μmol/L) and high accuracy (recovery rate of 95.85–98.06%). From mechanism study, the inhibition of TA on chromogenic reaction might be resulted from its antioxidation ability and the formation of Fe 3+ -TA complex. Finally, this method was applied to visual detection of TA content in different teas and red wines.
研究领域:
材料科学
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分析化学
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食品科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xiangqin Meng,Sijia Zou,Dandan Li,Jiuyang He,Ling Fang,Haojue Wang,Xiyun Yan,Demin Duan,Lizeng Gao
The coronavirus disease 2019 (COVID-19) pandemic has created a huge demand for sensitive and rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The current gold standard for SARS-CoV-2 detection is reverse transcription-polymerase chain reaction (RT-PCR)-based nucleic acid amplification. However, RT-PCR is time consuming and requires specialists and large instruments that are unattainable for point-of-care testing (POCT). To develop POCT for SARS-CoV-2, we combined recombinase polymerase amplification (RPA) and FeS 2 nanozyme strips to achieve facile nucleic acid amplification and subsequent colorimetric signal enhancement based on the high peroxidase-like activity of the FeS 2 nanozymes. This method showed a nucleic acid limit of detection (LOD) for SARS-CoV-2 of 200 copies/mL, close to that of RT-PCR. The unique catalytic properties of the FeS 2 nanozymes enabled the nanozyme-strip to amplify colorimetric signals via the nontoxic 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Importantly, the detection of clinical samples of human papilloma virus type 16 (HPV-16) showed 100% agreement with previous RT-PCR results, highlighting the versatility and reliability of this method. Our findings suggest that nanozyme-based nucleic acid detection has great potential in the development of POCT diagnosis for COVID-19 and other viral infections.
研究领域:
分子生物学
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病毒学
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即时诊断
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纳米技术
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-9
·
IF 6.232
Liu Qingju,He Zhaoying,Wang Hui,Feng Xiaoyuan,Han Ping
The fabrication of a magnetically controlled colorimetric aptasensor for chlorpyrifos is reported. The aptasensor was fabricated by the attachment of the colorimetric labels onto the magnetic carrier due to the hybridization reaction between the complementary DNA and aptamer. Chlorpyrifos detection was realized by monitoring the color changes of the TMB/H 2 O 2 solution before and after incubation of the aptasensor with chlorpyrifos via exposure to external magnetic force. The color change was monitored at 650 nm by UV-Vis spectrophotometer. Under the optimal conditions, this magnetically controlled Cu-MOF-based aptasensor showed a detection limit of 4.4 ng/mL with a linear range of 0–1250 ng/mL. The colorimetric aptasensor displayed high selectivity for chlorpyrifos toward other interfering pesticides. The aptasensor was successfully applied for the spiked test of chlorpyrifos in fruits and vegetable samples with good recovery, which were in agreement with data obtained by GC-MS analysis. This magnetically controlled Cu-MOF-based sensing strategy not only leads to development of efficient and facile phase separation, but also expands the MOF’s target scope from H 2 O 2 or glucose to pesticides.
Graphical abstract
研究领域:
分析化学
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环境科学
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纳米技术
·
食品安全
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DOI
PubMed
高影响因子
ACS Sensors
·
2021
·
IF 7.711
Li Xue,Nana Jin,Ruya Guo,Siyuan Wang,Wuzhen Qi,Yuanjie Liu,Yanbin Li,Jianhan Lin
In-field screening of foodborne pathogens plays an important role in ensuring food safety. Thus, a microfluidic biosensor was developed for rapid and sensitive detection of Salmonella using manganese dioxide nanoflowers (MnO2 NFs) for amplifying the biological signal, a microfluidic chip with a convergence–divergence spiral micromixer for performing automatic operations, and a smartphone app with a saturation calculation algorithm for processing the image. First, immune magnetic nanoparticles (MNPs), the sample, and immune MnO2 NFs were fully mixed and sufficiently incubated in the spiral micromixer to form MNP–bacteria–MnO2 sandwich complexes, which were magnetically captured in a separation chamber in the microfluidic chip. Then, a 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was injected and catalyzed by a MnO2 NF nanomimetic enzyme on the complexes, resulting in the production of yellow catalysate. Finally, the catalysate was transferred into a detection chamber and its image was measured and processed using the smartphone app to determine the number of bacteria. This biosensor was able to detect Salmonella from 4.4 × 101 to 4.4 × 106 CFU/mL in 45 min with a detection limit of 44 CFU/mL, and has the potential to provide a promising platform for on-site detection of foodborne bacteria.
研究领域:
生物技术
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微流控技术
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食品安全
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生物传感
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 10
·4437-4445
·
IF 2.7
Ran Zhang,Shaohong Zhang,Junhao Lu,Ying Wu,Jinjin Zhao,Zhijuan Wang
Nanozymes have attracted much attention as a new type of artificial enzyme with great development potential in recent years. Here, a cubic Co and N co-doped porous carbon material (Co-HPNC@NC) was synthesized through the calcination of PDA-modified bimetallic Co–Zn ZIFs. Co-HPNC@NC exhibits exceptionally high oxidase-mimicking activity and effectively oxidizes the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-colored oxidized TMB (oxTMB) without H2O2. However, the formation of oxTMB was inhibited by the addition of L-cysteine (L-Cys). Therefore, the blue color faded to colorless, causing a notable decrease in absorbance. Furthermore, Co-HPNC@NC showed good selectivity and anti-interference ability toward the detection of L-Cys. As a result, a sensitive and efficient colorimetric method has been developed to determine L-Cys with a low detection limit of 0.17 μM (3σ). It is believed that this colorimetric method for the detection of L-Cys based on Co-HPNC@NC will show potential applications in biosensors and medical diagnosis.
研究领域:
材料科学
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纳米技术
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分析化学
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生物传感
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医学诊断
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Wen Zheng,Dongjun Shen,Yadi Pan,Danyang Yi,Yijuan Long,Huzhi Zheng
The peroxidase-like activity of ficin is relatively low, which limits its application. It was found that thiol groups of ficin could inhibit its peroxidase-like activity. So, two procedures, i.e., direct blocking with N -ethylmaleimide (NEM), or using tris (2-carboxyethyl) phosphine hydrochloride (TCEP) to interrupt disulfide bonds then blocking thiol groups with NEM, were applied to block thiol groups of ficin, ficin-NEM (ficin-N) and ficin-TCEP-NEM (ficin-TN) were produced, respectively. The blocking of thiol groups accelerated the peroxidase activity dramatically. The peroxidase catalytic activity of ficin-N and ficin-TN toward the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation by H 2 O 2 was about 2.5-fold and 5-fold increase compared with ficin, respectively, which accompanied a color change from colorless to blue and followed classic Michaelis-Menten model. The kinetic parameters indicated that higher affinity of ficin-N ( K m = 0.31) and ficin-TN ( K m = 0.39) to H 2 O 2 compared with ficin ( K m = 0.58), and ficin-TN had the highest K cat which increased by 6.5 times and 4.5 times for TMB and H 2 O 2 , respectively. According to these findings, a colorimetric method with high sensitivity for the detection of biothiols was developed due to sulfhydryl compounds inhibited the peroxidase activity of ficin. Comparing with ficin and ficin-N, ficin-TN had the widest detection range (0.01–16 μM) and the lowest detection limit (3 nM). The practical applications of ficin-TN for biothiol determination in human serum samples have been demonstrated with satisfactory results. Ficin-N and ficin-TN are promising to apply to the bioanalysis.
研究领域:
生物化学
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分析化学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-11
·
IF 6.408
Zhou Hang-Yu,Peng Li-Jing,Tian Tao,Zhang Wei-Yi,Chen Guo-Ying,Zhang Hao,Yang Feng-Qing
An effective, selective, and multicolor colorimetric assay for Cu 2+ detection based on the regulation of peroxidase-like nanozyme-mediated etching of gold nanorods (Au NRs) is proposed. Cu 2+ -creatinine complex is selected as the nanozyme that exhibits excellent peroxidase-like activity even in the case of low concentration of Cu 2+ , which can catalyze 3,3,5,5-tetramethylbenzidine (TMB) to produce oxidized TMB (TMB + ) in the presence of hydrogen peroxide, and TMB + is oxidized to generate TMB 2+ after adding H + , and the TMB 2+ can etch Au NRs. The determination of Cu 2+ is achieved based on the blue shift of the longitudinal localized surface plasmon resonance peak of Au NRs. Under the optimal conditions, the developed colorimetric assay exhibits high sensitivity for the detection of Cu 2+ (limit of detection is 0.034 μM) with a wide linear range of 0.05–4.0 μM ( R 2 = 0.987). The solution shows a rainbow-like color in response to the increase of Cu 2+ concentration, which can realize the semi-quantitative detection of Cu 2+ by naked eyes. In addition, the developed method exhibits excellent selectivity for Cu 2+ -detection. The established method was used for the determination of Cu 2+ in lake water, soil, and normal human serum with satisfactory recovery of spiked samples. Graphical abstract
研究领域:
分析化学
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纳米技术
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环境科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-13
·
IF 5.3
Gao Xue,Liu Lu,Jia Mu,Zhang Hongmei,Li Xuepeng,Li Jianrong
A dual-mode (colorimetric/fluorescence) nanoenzyme-linked immunosorbent assay (NLISA) was developed based on Au-Cu nanocubes generating Prussian blue nanoparticles (PBNPs). It is expected that this method can be used to detect the residues of sulfonamides in the field, and solve the problem of long analysis time and high cost of the traditional method. Sulfadimethoxine (SDM) was selected as the proof-of-concept target analyte. The Au-Cu nanocubes were linked to the aptamer by amide interaction, and the Au-Cu nanocubes, SDM and antibody were immobilized on a 96-well plate using the sandwich method. The assay generates PBNPs by oxidising the Cu shells on the Au-Cu nanocubes in the presence of hydrochloric acid, Fe 3+ and K 3 [Fe (CN) 6 ]. In this process, the copper shell undergoes oxidation to Cu 2+ and subsequently Cu 2 + further quenches the fluorescence of the carbon point. PBNPs exhibit peroxidase-like activity, oxidising 3,3’,5,5’-tetramethylbenzidine (TMB) to OX-TMB in the presence of H 2 O 2 , which alters the colorimetric signal. The dual-mode signals are directly proportional to the sulfadimethoxine concentration within the range 10 − 3 ~10 − 7 mg/mL. The limit of detection (LOD) of the assay is 0.023 ng/mL and 0.071 ng/mL for the fluorescent signal and the colorimetric signal, respectively. Moreover, the assay was successfully applied to determine sulfadimethoxine in silver carp, shrimp, and lamb samples with satisfactory results.
研究领域:
分析化学
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纳米技术
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生物化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 5
·1-9
·
IF 5.833
Zhang Wenxian,Li Xiuping,Cui Tianyu,Li Shenchang,Qian Yuqing,Yue Yu,Zhong Wenying,Xu Bo,Yue Wanqing
Using an ultrasonication-assisted liquid exfoliation method, we have synthesized PtS 2 nanosheets with good reproducibility. Herein, intrinsic peroxidase-like activity was for the first time demonstrated for PtS 2 nanosheets, which can catalyze H 2 O 2 oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a colored solution. The catalytic mechanism of PtS 2 nanosheets was investigated, which indicated that acceleration of the electron transfer between TMB and H 2 O 2 was the main reason for the peroxidase-like activity of PtS 2 nanosheets. Based on these observations, we exploited PtS 2 nanosheets integrated into dopamine-functionalized hyaluronic acid (HA-DA) hydrogel microspheres by droplet microfluidics to construct PtS 2 nanosheet– and PtS 2 @HA-DA microsphere–based sensors for highly sensitive determination of H 2 O 2 . When coupled with glucose oxidase, we further developed two glucose sensors based on the above two methods. Among them, the linearity of the PtS 2 nanosheet–based spectrophotometry was in the range of 0.5 to 150 μM and the limit of detection as low as 0.20 μM. The linearity of the microsphere-based colorimetry was in the range 200 to 12,000 μM with a detection limit of 29.95 μM. Both of the glucose sensors can be applied to the determination of glucose in human serum with reliable results and reproducibility. Graphical abstract
研究领域:
纳米技术
·
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Chu Shushu,Xia Mingyuan,Xu Peng,Lin Dalei,Jiang Yuanyuan,Lu Yizhong
Although traditional Fe-based nanozymes have shown great potential, generally only a small proportion of the Fe atoms on the catalyst’s surface are used. Herein, we synthesized single-atom Fe on N-doped graphene nanosheets (Fe-CNG) with high atom utilization efficiency and a unique coordination structure. Active oxygen species including superoxide radicals (O 2 •− ) and singlet oxygen ( 1 O 2 ) were efficiently generated from the interaction of the Fe-CNG with dissolved oxygen in acidic conditions. The Fe-CNG nanozymes were found to display enhanced oxidase-like and laccase-like activity, with V max of 2.07 × 10 −7 M∙S −1 and 4.54 × 10 −8 M∙S −1 and K m of 0.324 mM and 0.082 mM, respectively, which is mainly due to Fe active centers coordinating with O and N atoms simultaneously. The oxidase-like performance of the Fe-CNG can be effectively inhibited by ascorbic acid (AA) or hydroquinone (HQ), which can directly obstruct the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, a direct and sensitive colorimetric method for the detection of AA and HQ activity was established, which exhibited good linear detection and limit of detection (LOD) of 0.048 μM and 0.025 μM, respectively. Moreover, a colorimetric method based on the Fe-CNG catalyst was fabricated for detecting the concentration of AA in vitamin C. Therefore, this work offers a new method for preparing a single-atom catalyst (SAC) nanozyme and a promising strategy for detecting AA and HQ.
研究领域:
催化
·
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Jie Dong,Laixi Zhang,Wei Li,Xin Hu,Anyi Chen,Chaorui Li
In this study, a facile and cost-effective hydrothermal synthesis method was used to synthesize zinc oxide nanoflowers modified by reduced graphene oxide, and subsequently, trimetallic AuPtRu nanoparticles(AuPtRuNPs) were supported via the reduction method for high-sensitivity colorimetric detection of H2O2 in weakly acidic solutions. Compared to monometallic and bimetallic nanoparticles, trimetallic nanoparticles exhibit significant synergistic effects and enhanced catalytic activity. After providing a three-dimensional structure with multiple pores by zinc oxide and enhancing electron transfer ability by reduced graphene, the trimetallic nanocomposites (AuPtRu/ZnO-rGO) exhibited excellent peroxidase-mimicking activity, which can effectively catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue oxidation product (oxTMB) in the presence of H2O2. Compared to horseradish peroxidase (HRP), AuPtRu/ZnO-rGO demonstrated significantly enhanced catalytic velocity (Vmax = 6.16 × 10–8 M/s) and affinity (Km = 0.02) for H2O2. The study of the catalytic mechanism showed that trimetallic Au, Pt, and Ru could effectively catalyze H2O2 to produce hydroxyl radicals (•OH) to accelerate the oxidation of TMB and enhance the peroxidase-mimicking activity of the AuPtRu/ZnO-rGO nanocomposites. The results showed that the as-synthesized hydrangea-like AuPtRu/ZnO-rGO nanocomposites showed enhanced peroxidase-mimicking activity. It could be used for the colorimetric detection of H2O2 in the range 5–1000 μM with a LOD of 3.0 μM (S/N = 3), and the recoveries are 93.0–101.7%. In addition, the AuPtRu/ZnO-rGO nanocomposites have good applicability for sensitive colorimetric determination of H2O2 in milk, and it has broad application prospects as a multifunctional sensing platform in the food processing industry.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
生物催化
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Dongxue Sun,Tianli Liu,Yuming Dong,Qingyun Liu,Xiuming Wu,Guang-Li Wang
Herein, an innovative dehydrogenase invoked tri-enzyme cascade has been successfully constructed and utilized as a powerful and universal colorimetric strategy for dehydrogenase-based bioassays. Specifically, the glucose-6-phosphatedehydrogenase (G6PD) catalyzes the specific dehydrogenation of glucose-6-phosphate (G-6-P) by consuming the oxidized form of β-nicotinamide adenine dinucleotide phosphate (NADP + ) to generate NADPH. And the formed NADPH initiates the irreversible hydroxylation of p-hydroxybenzoate by p-hydroxybenzoate hydroxylase (PHBH) to generate 3,4-dihydroxybenzoate (DHB), which results in the subsequent in situ generation of a photoresponsive nanozyme of DHB coordinated SrTiO 3 (SrTiO 3 /DHB) nanosheets. The photoresponsive nanozyme has intriguing interfacial charge transfer (ICT) characteristics and thus realizes the efficiently catalytic oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under light irradiation for signal readout. Exemplified by G6PD and G-6-P as analytes, the dehydrogenase invoked tri-enzyme cascade achieved efficient signal amplification towards the ultrasensitive colorimetric bioassays. This study opens up the ICT transitions of SrTiO 3 for innovative dehydrogenases-involved enzyme cascades. In addition, it is an entirely new method for dehydrogenases-based bioassays, with the distinctive merits of facile operation, rapid response, high sensitivity, and naked-eye readout. Importantly, it is extensible as a general basis for other numerous targets of interest because of the large numbers of dehydrogenases available for catalysing different reactions.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
生物催化
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）