RSC Advances
·
2017
·Vol. 7
·期 15
·8823-8831
·
IF 3.108
Tsogzolmaa Ganbold,Gerile Gerile,Hai Xiao,Huricha Baigude
RNA interference (RNAi) has shown great potential for clinical treatment of a variety of diseases. As an effective post-transcriptional regulation, RNAi induces degradation of specific target mRNA, leading to a decreased level of disease related gene product, which could be resistant to conventional small molecule therapeutics. Because of the relatively small size of short interfering RNA (siRNA) as well as it's robustness in the design of efficient 20–22 nucleotide sequences targeting any known gene, delivery of siRNA in vitro and in vivo has achieved tremendous success by the design and fabrication of various carrier materials including peptides, lipids, and polymers. While siRNA delivery efficiency of these materials proved to be appreciable, their tissue specificity, biocompatibility and cytotoxicity still need much improvement. We report a novel siRNA delivery material designed by optimization of our previously reported peptidomimetic based lipoplex. The novel lipoplex, which bears a D-amino acid based dipeptide head group, possesses an ideal balance of increased stability in a tissue environment, and enhanced efficiency of tissue specific delivery as well as minimized cytotoxicity. The novel lipoplex carrying siRNA efficiently targeted mouse liver, initiated RNAi and knocked down the apoB dose dependently, which is confirmed by both RT-qPCR and Western blotting. Moreover, the novel lipoplex does not induce any apparent cytotoxicity, as confirmed by the measurement of liver enzyme levels in serum.
研究领域:
分子生物学
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药学
·
生物技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Juan Li,Ming-Chun Lai,Ya-Meng Zhong,Ya-Ling Chen,Na Wu,Wei Chen,Hua-Ping Peng
Monitoring D-amino acids concentrations has essential implications for gastric cancer diagnosis and treatment, especially in the non-invasive detection of gastric cancer. However, it remains a challenge to establish a high-performance detection method for D-amino acids. Here, a highly sensitive and selective d -alanine (D-Ala) ECL assay strategy via a turn-off-on split-type electrochemiluminescence (ECL) platform has been proposed. We found that hydrogen peroxide (H 2 O 2 ) could be used as an efficient etching agent to turn on the MnO 2 /gold nanocluster (AuNC)-based ECL nanoswitch platform. Based on abovementioned characteristics, we extended it to the D-amino acids assay since the enzymatic reaction between D-Ala and D-amino acid oxidase (DAAO) generates H 2 O 2 . Based on the abovementioned characteristics, this ECL sensing platform achieved a preferable linear-dependent curve in the detection range of 1.0 × 10 − 10 ∼1.0 × 10 − 3 mol L −1 , and realized the detection of D-Ala as low as 2.2 × 10 − 11 mol L −1 (S/N = 3). Furthermore, the proposed ECL biosensor showed excellent selectivity, stability, and reproducibility. Together with its powerful performance, this strategy could test D-Ala in saliva samples, which suggested that this ECL assay platform shows great prospect in disease diagnosis. We ascribe the high sensitivity and good anti-interference capability of the sensor to the combination of specific enzyme catalysis reaction, high-efficiency split-type AuNC probe-based ECL technique and the highly etching efficiency of H 2 O 2 to MnO 2 nanomaterials on electrode surface. In our perception, this H 2 O 2 mediated split-type ECL sensing platform provides a viable tool in ECL based bioananlysis. Therefore, our proposed approach not only provides a strategy for developing a high-performance platform for D-Ala detection, but also establishes a framework for the detailed design and development of ECL platform for other biological assays.
研究领域:
生物医学
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分析化学
·
癌症诊断
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Songlin Chen,Zhihao Shen,Shuming Wen,Guang Han,Qicheng Feng
Flotation separation of Cu–Fe sulfide minerals typically necessitate the addition of substantial quantities of lime to depress pyrite flotation. Because lime can harm the environment, there is an urgent need to develop novel environmentally friendly depressants that are non-toxic and biodegradable. In this study, we investigated an environmentally friendly pyrite depressant containing amino and carboxyl groups, namely D-arginine (DA). The effect of DA on the surface hydrophobicity of Cu–Fe sulfide minerals and thus collector adsorption was investigated by analyzing zeta potentials, adsorption capacities, and contact angles. Additionally, changes in the surface properties of the minerals induced by DA were examined using X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectroscopy (ToF-SIMS), and atomic force microscopy (AFM). Flotation tests revealed that 2 × 10 –4 mol/L DA reduced the flotation recovery of pyrite to 9.96 % at low alkalinity while leaving chalcopyrite recovery unchanged. The results of mineral surface analysis indicated that DA exhibited significantly stronger adsorption on the pyrite surface than on the chalcopyrite surface. Furthermore, DA effectively reduced the adsorption of the collector on the surface of pyrite, thereby weakening pyrite floatability at low alkalinity. Therefore, DA is a promising alternative depressant for the flotation separation of chalcopyrite from pyrite.
研究领域:
矿物加工工程
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环境科学
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材料化学
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2016
·
IF 2.567
Binglin Li,Jiao Wang,Xiaoli Zhang,Binxia Zhao
Phospholipase D (PLD) was immobilized in a simple and effective way by adsorption and precipitation of the enzyme, followed by chemical cross-linking to form an “enzyme net” covering the surface of nonporous silicon dioxide nanoparticles. For catalyzing transphosphatidylation to produce phosphatidylethanolamine (PE), at pH 6.0 and 35 °C (the optimum operational conditions), the specific activity of immobilized PLD reached 15872 U/gprotein, which was approximately 1.15 times higher than the maximum value of specific activity of free PLD (13813 U/gprotein). A kinetic study demonstrates immobilized PLD had increases in catalytic activity and enzyme–substrate affinity. In addition, the thermostability and pH tolerance were significantly enhanced compared with free PLD. The half-life of immobilized PLD was significantly increased from 30 to 70 days at 4 °C (approximately 2.3 times). This novel method has been proven to be suitable for the production of robust biocatalysts.
研究领域:
生物化学
·
纳米生物学
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DOI
高影响因子
Nano Research
·
2023
·Vol. 16
·期 8
·10748-10755
·
IF 9.9
Li Junhua,Jiang Yi,Zhang Xu,Huo Yidan,Du Fanglin,Tuo Yongxiao,Guo Zhiyan,Chen Dawei,Chen Shenghua
The electrooxidation of the alcohol and aldehyde molecules instead of water coupled with H 2 production has been proven to be effective for producing high-value fine chemicals under alkaline conditions. It is also noteworthy that under acidic conditions, the stability of non-noble metal water oxidation catalysts remains a great challenge due to the lattice oxygen mechanism. Hence, we coupled the biomass-derived glucose oxidation for high-value D-glucaric acid (GRA) with ultra-durable hydrogen in acid solution over a Yb-MnO 2 catalyst. The Mn 3+ regulated by Yb atoms doped in MnO 2 can effectively optimize the adsorption and desorption processes of the alcohol and aldehyde group and improve the intrinsic activity but cannot for H 2 O. The catalyst exhibited extremely high activity and stability after 50 h for glucose oxidation, inhibiting the lattice oxygen process and MnO 4− formation, while the activity was quickly lost within 0.5 h for water oxidation. Density functional theory (DFT) calculations further demonstrated that glucose oxidation reaction proceeds preferentially due to the oxidation of aldehyde group with lower adsorption-free energy (−0.4 eV) than water (Δ G > 0 eV), avoiding the lattice oxygen mechanism. This work suggests that biomass-derived glucose oxidation not only provides a cost-effective approach for high-value chemicals, but also shows an extremely potential as an alternative to acidic oxygen evolution reaction (OER) for ultradurable H 2 production.
研究领域:
生物化学
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催化化学
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2024
·
IF 5.8
Liwei Liang,Songlin Cai,Lan Zhang,Ke Sun,Ziying He,Libin Zhang,Ciyuan Huang,Min Long,Hongxiang Zhu,Bingsuo Zou,Tao Liu,Shuangfei Wang
Phenol has various applications in industry, due to its hazardousness, solubility, and volatility, it persists in water and poses a risk to human health. People are excited by the progress of photodegradation technology that facilitates water pollution treatment. The effect of side-chain engineering is proven to enhance photodegradation performance by improving optoelectronic properties. Therefore, here is a strategy that introduces functional groups to change the electron cloud density, achieving excellent optoelectronic properties evidenced by efficient exciton dissociation, separation, and fine-tuned energy level. Subsequently, by coupling of photocatalysis and adsorption, the donor/acceptor (D/A) heterojunction photocatalysts (PBDB-T: BTTIC-TT, PBDB-T: BTTIC-Ph) are designed via loading on coconut-shell carbon to process phenol. The results indicate that the champion photocatalyst PBDB-T: BTTIC-TT possesses superior optoelectronic properties, which can be attributed to the stronger electron-withdrawing ability of thieno[3,2- b ]thiophene than benzene. Consequently, the synergistic efficiency of adsorption and photocatalysis of phenol (10, 20 mg/L) are removed within 10 mins over 90 % which is still valid after twenty cycles. Surprisingly, a high concentration of phenol (100 mg/L) also exhibited excellent removal efficiency of over 80 % within 60 min.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Dan Zhao,Jingjing Huang,Juan Li,Xiao Ma,Fang Wang,Honglei Zhang,Jiaze Xie,Jian Sun,Chuanxia Chen
Dual-readout protocols with two signal transduction channels can significantly improve the detection precision and diversity. In contrast to the previous tedious and time-consuming preparation of two response materials, a convenient and commercially available small molecule o -Phenylenediamine (OPD)-based dual-mode system was developed for the ultrasensitive detection of D-penicillamine (D-PA). Fortunately and interestingly, it is demonstrated that chloride ions (Cl − ) could largely enhance the oxidative ability of copper ions (Cu 2+ ) and improve the reaction rate and efficiency of the Cu 2+ -OPD system, leading to the boosted colorimetric and fluorescent signals and shorter reaction time. Meanwhile, D-PA could strongly bind with Cu 2+ to form a stable Cu 2+ -D-PA complex, thereby inhibiting the aforementioned oxidation process and exhibiting decrease of absorbance values and fluorescence intensity. Inspired by the excellent analytical performance, an “INH” molecular logic gate was devised by employing Cl − and D-PA as the inputs, and the dual-mode strategy has been successfully employed to probe D-PA in tablets and human serum samples. Such cost-effective yet efficacious analytical tool not only improve the sensing performance of the Cu 2+ -OPD reaction system, but also enhance the detection sensitivity for D-PA and promote the application and development of OPD-based reactions.
研究领域:
分析化学
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生物医学工程
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Jimei Ma,Heng Zhang,Fangfang Peng,Xiaoqing Yang,Zi-Long Li,Linhao Sun,Hong Jiang
Carbon dots (CDs) were synthesized from p -aminophenol and ethylenediamine via one-step under mild condition and used as a sensitive fluorescent nanoprobe for the activity determination of N -acetyl-β- d -glucosaminidase (NAG). In this assay, p -nitrophenol was in situ produced from p -nitrophenyl-β-D- N -acetyl-glucosaminide, which was exclusively hydrolyzed by NAG. The UV absorption peak of p -nitrophenol (maximum at 400 nm) overlapped the excitation peak of CDs with maximum wavelength at 415 nm, which caused the fluorescence decline of CDs based on inner filter effect. The activity of NAG was determined by the fluorescence changes. The assay is highly sensitive to NAG with a low detection limit of 0.75 U L −1 (K = 3) and showed a good linear relationship in the range from 1 to 45 U L −1 . This CDs nanoprobe was successfully applied for the determination of NAG activity in human serum and urine samples.
研究领域:
生物化学
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医学诊断
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纳米技术
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产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2018
·
IF 3.977
Ying Xiao,Jialin Dong,Zhiting Yin,Qiguo Wu,Yiming Zhou,Xiaoli Zhou
To elucidate the possible mechanisms for the preventive effect of procyanidin B2 on aging, a combined analysis of metabolic profile and gut microbiome was carried out in the present study. The mimetic aged mice induced by d -galactose injection (500 mg/kg, sc daily), and the preventive group was fed with the diet plus 0.2% procyanidin B2. After 7 weeks of treatment, the spatial memory was assayed using the Morris water maze test. Procyanidin B2 significantly ameliorated the impaired memory and antioxidant abilities induced by d -galactose. Furthermore, metabolomics analysis of plasma based on LC/Q-TOF-MS demonstrated that phosphatidyl cholines , oleic acid , linoleic acid , carnitine , pantothenic acid , and taurocholic acid were significantly increased in the mice treated with procyanidin B2, and pyruvic acid , hydroxybutyric acid , hippuric acid , and cholic acid were decreased significantly. Together, gut microbiome analysis using Illumina sequencing showed that there were significant differences in the Firmicutes / Bacteroidetes ratio and abundance of Roseburia , Lachnospiraceae , and Bifidobacterium between the aging and supplemental procyanidin B2 groups. In summary, procyanidin B2 possessed potential prevention of the cognitive and oxidative impairment via the metabolic pathway regulation related to citrate cycle, fatty acid, and bile acid in the aged mice, accompanied by remodeling the gut flora.
研究领域:
生物化学
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分子生物学
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老年医学
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营养学
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产品关联
DOI
PubMed
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2022
·Vol. 38
·期 8
·1-11
·
IF 4.253
Xue Kai,Liu Chun-Li,Yang Yankun,Liu Xiuxia,Zhan Jinling,Bai Zhonghu
d -allulose is a rare low-calorie sugar that has many fundamental biological functions. d -allulose 3-epimerase from Agrobacterium tumefaciens (AT-DAEase) catalyzes the conversion of d -fructose to d -allulose. The enzyme has attracted considerable attention because of its mild catalytic properties. However, the bioconversion efficiency and reusability of AT-DAEase limit its industrial application. Magnetic metal–organic frameworks (MOFs) have uniform pore sizes and large surface areas and can facilitate mass transport and enhance the capacity for enzyme immobilization. Here, we successfully encapsulated cobalt-type AT-DAEase into the cobalt-based magnetic MOF ZIF-67@Fe 3 O 4 using a self-assembly strategy. We confirmed the immobilization of enzyme AT-DAEase and characterized the enzymatic properties of the MOF-immobilized AT-DAEase@ZIF-67@Fe 3 O 4 . The AT-DAEase@ZIF-67@Fe 3 O 4 nanoparticles had higher catalytic activity (65.1 U mg −1 ) and bioconversion ratio (38.1%) than the free AT-DAEase. The optimal conditions for maximum enzyme activity of the AT-DAEase@ZIF-67@Fe 3 O 4 nanoparticles were 55 °C and pH 8.0, which were significantly higher than those of the free AT-DAEase (50 °C and pH 7.5). The AT-DAEase@ZIF-67@Fe 3 O 4 nanoparticles displayed significantly improved thermal stability and excellent recycling performance, with 80% retention of enzyme activity at a temperature range of 45–70 °C and > 45% of its initial activity after eight cycles of enzyme use. The AT-DAEase@ZIF-67@Fe 3 O 4 nanoparticles have great potential for large-scale industrial preparation of d -allulose by immobilizing cobalt-type AT-DAEase into magnetic MOF ZIF-67@Fe 3 O 4 .
研究领域:
生物化学
·
食品科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2022
·
IF 5.723
Chaofeng Chang,Yuhua Xie,Fang Luo,Shaowei Zhou,Zehui Yang
To economically realize proton electrolyte membrane water splitting technology, stable and high-performance oxygen evolution reaction (OER) electrocatalyst is significantly demanded. In this work, we invite tungsten atoms to metallic iridium (Ir) to weaken s-d hybridization of Ir-Ir bond due to the higher electronegativity accepting electron from Ir atoms; as a result, the adsorbed O* species become electrophilic favorable for the nucleophilic attack by H 2 O leading to 22 % decrement in energy barrier for the formation of OOH* species. Therefore, iridium-tungsten (IrW) electrocatalyst exhibits 3.7-fold higher turnover frequency (TOF) than metallic Ir at overpotential of 370 mV in acidic OER catalysis. Moreover, stable OER performance is recorded due to the high cohesive energy of tungsten atoms. Additionally, TOF of IrW electrocatalyst reaches 10.1 s −1 at overpotential of 100 mV in acidic hydrogen evolution reaction (HER) test, 7.6-time higher than Pt/C and metallic Ir. IrW electrocatalyst only needs 1.52 V to attain 10 mA cm −2 in acidic overall water splitting.
研究领域:
材料科学
·
电化学
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DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2023
·
IF 22.1
Sundaram Chandrasekaran,Tingting Ma,Zhichang Hu,Qianying Liu,Chao Zhan,Yan Li,Chris Bowen,Huidan Lu,Yongping Liu
Chevrel-phase metal sulfides are known to be promising materials for energy conversion and storage applications. However, a detailed understanding of the intrinsic kinetic mechanisms of electrocatalytic bifunctional hydrogen and oxygen evolution reactions (HER/OER) on NiMo 3 S 4 -based Chevrel-phases is lacking. Herein, novel ultrathin self-assembled nanosheets of NiMo 3 S 4 are coupled with transition metal atoms (M/N-NiMo 3 S 4 ; where M = Co, Fe, and Cu) were formed by a facile hydrothermal approach. Notably, the Co/N-NiMo 3 S 4 electrocatalyst exhibits excellent performance in terms of ultralow overpotentials of 78, 208, 282, and 307 mV at 10, 100, 500, and 1000 mA cm −2 for the HER; and 186, 204, and 225 mV at 50, 100, and 300 mA cm −2 for the OER, respectively. Experimental and first principle calculations demonstrate that Co atoms coupling with edge Ni atoms results in d‐electron delocalization on Co/N-NiMo 3 S 4 , signifying the efficient charge transfer to improve overall water electrolysis. In addition, an upshift in the d‐band center of Co/N-NiMo 3 S 4 can optimize the free energies of a variety of reaction intermediates for water adsorption and dissociation; thereby facilitating the robust alkaline overall water electrolysis at 1.47 V. This work therefore greatly deepens the understanding of the bifunctional hydrogen and oxygen evolution reaction of Chevrel-phase electrocatalysts.
研究领域:
材料科学
·
电化学
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催化
·
能量转换与存储
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）