CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Yi Zhang,Da Luo,Yin Zhang,Qi-Hai Zhang,Qing-Yuan Ji,Shi-Kang Zhou,Shan Huang,Li-li Li,Feng Lu,Wei-Feng Yao,Fang-Fang Cheng,Jun-Jie Zhu,Li Zhang
Upconversion nanoparticles (UCNPs) are promising materials for imaging biomolecules and predicting the progression of diseases, especially for tumors. However, conventional luminescence resonance energy transfer (LRET) based nanosensors remain challenging with complicated modification and unsatisfactory sensitivity. Herein, a sensing platform integrating confined emission effect and cycling amplification strategy into one substrate with high LRET efficiency and sensitivity has been developed for the in - vitro and in - vivo detection of microRNA-21. Washing UCNPs with hydrochloric acid was conducted for electrostatic absorption of 8–17 DNAzyme labeled with BHQ1 (8–17 DNAzyme-BHQ1) and complementary capturing template-aDNA3 complex (CPT-aDNA3) to form energy donor–acceptor pair (UCNPs-BHQ1) and capture miR-21. It not only avoids the complex modification procedure but also shortens the distance between the donor (UCNPs) and the acceptor (BHQ1). The quenching efficiency of 60.94 % was obtained. Targeted miR-21 could bind with CPT to release aDNA3 that can unlock 8–17 DNAzyme-BHQ1 to form aDNA3-DNAzyme-BHQ1 duplex. This aDNA3-DNAzyme-BHQ1 duplex owning -rA- group can be cleaved by Zn 2+ so that UCNPs restored green UCL emission because of the desorption of BHQ1. aDNA3 becomes dissociative again to trigger the next cycle. This [email protected] /CPT- aDNA3 achieved a wide linear range from 4 × 10 -14 M to 1 × 10 -8 M with a low limit detection of 31 fM for the sensitive detection of miR-21 in vitro . Furthermore, it was successfully applied to the real-time intracellular detection of miR-21 in HeLa cells and in - vivo monitoring of miR-21 expression in KS-01 treated tumor-bearing mouse. This proposed DNA-conjugated upconversion nanoamplicon provides new sights into monitoring of anti-cancer active ingredients efficacy and the progress evaluation of miRNA-related diseases.
研究领域:
纳米技术
·
生物医学工程
·
分子生物学
·
癌症研究
·
生物成像
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高影响因子
Journal of Materials Chemistry B
·
2023
·
IF 7
Xiaoxue Dong,Zongwei Zhu,Qian Sun,Hongqian Zhang,Chuanxu Yang
The development of a theranostic platform that integrates both diagnostic and therapeutic capabilities is in great need for precise and personalized medicine. Here, we present a novel nanoplatform (AuNS@CS-hpDNA) formulated by chitosan functionalized gold nanostar composites and further complexed with fluorescent hairpin DNA (hpDNA) probes for tumor-related miRNA imaging and photothermal therapy (PTT). The optimized AuNS@CS-hpDNA nanoplatform mediated efficient hpDNA probe loading and intracellular delivery. Subsequently, the cytosol transfer of the hpDNA probe enabled specific hybridization using the targeted miRNA, which triggered the recovery of fluorescence for the precise detection of biomarker miR21 in living cells and realized the distinguishing cancer cell line MCF-7 and normal cells. Meanwhile, the AuNS@CS-hpDNA nanoplatform exhibited excellent photothermal conversion properties, which induced efficient cancer cell killing under laser irradiation. Thus, the developed AuNS@CS-hpDNA nanoplatform could simultaneously realize the precise detection of cancer cells and accurately initiate efficient PTT, which represents a promising strategy for precise cancer therapy.
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DOI
PubMed
高影响因子
Emergent Materials
·
2023
·
IF 3.8
Shen Yuxin,Zhang Yao,Gao Xiyue,Shang Mengdi,Cai Yanfei,Yang Zhaoqi
The most prevalent malignant neoplasm identified in females is breast cancer. Multidrug resistance is a significant obstacle for breast cancer chemotherapy, despite the availability of several therapies. This article describes the development of a nanocomposite based on ZIF-90 to reduce multidrug resistance in breast cancer cells. Synthesized ZIF-90 is utilized to transport MiR-489 and Adriamycin into drug-resistant cancer cells. The epithelial-mesenchymal transition that is initiated by TGF-β is dependent on Smad3, and MiR-489 may target Smad3. MiR-489 ultimately suppresses the epithelial-mesenchymal transition pathway, hence increasing the efficacy of Adriamycin therapy. The results of Western blot analysis indicated that the Smad3 is effectively reduced by MiR-489. The cell viability assay showed that the IC 50 of nanocomposite to the drug-resistant breast cancer cells (1.032 μg/mL) was nearly 40 times lower than the IC 50 of ADM to the same cells (39.87 μg/mL), and was closer to the IC 50 of nanocomposite to the non-drug-resistant cells (0.49 μg/mL). These findings conclusively shown that our developed nanocomposite has ameliorated the multidrug resistance issue.
研究领域:
肿瘤学
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纳米技术
·
分子生物学
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DOI
高影响因子
ANALYTICAL SCIENCES
·
2023
·
IF 1.6
Xi Huai,Liang Xiaolin,Huang Guidan,Liang Jinhua,Li Dandan,Wen Qilin,Zhang Yun,Xiao Xiaofen,Zhu Wenyuan
The rapid and accurate detection of miRNAs is of great significance for early diagnosis and treatment of cancer. Hence, a novel enzyme-free and label-free electrochemical biosensor based on bio-barcode amplification for detecting miRNAs was presented. Sandwich structures constructed of magnetic nanoparticles modified with DNA probes, gold nanoparticles with numerous barcoded DNA strands that hybridized with target miRNAs were fabricated as the amplifier. The released barcoded DNA strands then acted as the secondary targets and triggered the electrochemical sensor with a significant electrochemical response. A highly sensitive (detection limit of 0.24 fM) and selective electrochemical miRNA detection was realized, which has great potential for application in miRNA-related clinical diagnosis and biochemical research. Graphical abstract A novel biosensor based on bio-barcode amplification for the electrochemical detection of miR-7a was successfully developed.
研究领域:
生物化学
·
分子生物学
·
医学诊断
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2014
·
IF 5.825
Mo Wang,Zhengliang Fu,Bingchen Li,Yunlei Zhou,Huanshun Yin,Shiyun Ai
Taking advantage of the special exodeoxyribonuclease activity of T7 exonuclease, a simple, sensitive, selective, and label-free microRNA biosensor based on the cyclic enzymatic amplification method (CEAM) has been proposed. First, thiol functionalized DNA probes were assembled onto a gold nanoparticles modified gold electrode surface through a Au–S bond, followed by hybridizing with target miRNA. Subsequently, DNA in RNA/DNA duplexes was digested by T7 exonuclease, which can release the microRNA molecules from the electrode surface and return into the buffer solution. Meanwhile, the released microRNA can further hybridize with the unhybridized DNA probes on the modified electrode surface. On the basis of it, an isothermal amplification cycle is realized. The T7 exonuclease-assisted CEAM achieved a low detection limit of 0.17 fM. Moreover, this assay presents excellent specificity with discriminating only a single-base mismatched microRNA sequence. Furthermore, this work can also be applied to detect avian leukemia based on the decreased expression level of microRNA-21.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2025
·
IF 8.9
Xinlan Zhu,Ziyan Zhang,Ruiyang Ma,Huachu Chen,Yi Zheng,Su Zeng,Zheyong Li,Sheng Cai
A label-free detection technique for microRNA (miRNA) was developed based on target replacement cycling, polymerization and isomerization cyclic amplification (PICA), and G/Hemin DNAzyme signal output. The designed hairpin probe undergoes cyclic alternation of polymerization and isomerization catalyzed by Bst DNA polymerase, enabling target replacement cycling and continuous self-extension of single-stranded DNA (ssDNA) strands. This process generates abundant G-quadruplex sequences that bind to Hemin, achieving label-free detection of miRNA through catalytic reactions. Its key advantages include a specific primer self-extension mechanism that avoids spurious amplification, one step isothermal design simplifies workflow and enhances system robustness, adapting to diverse detection scenarios, and label-free detection strategy that substantially reduces cost. Therefore, it is poised to become a reliable tool for clinical miRNA analysis, offering potential guidance for the early diagnosis, treatment, and prognosis of tumor biomarkers.
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高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Xuehong Min,Mengshi Zhang,Fujian Huang,Xiaoding Lou,Fan Xia
Enzyme-assisted detection strategies of microRNAs (miRNAs) in vitro have accomplished both great sensitivity and specificity. However, low expression of miRNAs and a complex environment in cells induces big challenges for monitoring and tracking miRNAs in vivo. The work reports the attempt to carry miRNA imaging into live cells, by enzyme-aided recycling amplification. We utilize facile probes based yellow aggregation-induced emission luminogens (AIEgens) with super photostable property but without quencher, which are applied to monitor miRNAs not only from urine sample extracts (in vitro) but also in live cells (in vivo). The assay could distinguish the cancer patients’ urine samples from the healthy urine due to the good specificity. Moreover, the probe showed much higher fluorescence intensity in breast cancer cells (MCF-7) (miR-21 in high expression) than that in cervical cancer cells (HeLa) and human lung fibroblast cells (HLF) (miR-21 in low expression) in more than 60 min, which showed the good performance and super photostability for the probe in vivo. As controls, another two probes with FAM/Cy3 and corresponding quenchers, respectively, could perform miRNAs detections in vitro and parts of in vivo tests but were not suitable for the long-term cell tracking due to the photobleach phenomena, which also demonstrates that the probe with AIEgens is a potential candidate for the accurate identification of cancer biomarkers.
研究领域:
分子生物学
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癌症研究
·
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Zhen Wang,Yucui Zhang,Xiuzhong Wang,Lei Han
Considering DNA-based homogeneous electrochemical assay allows identification of targets to be carried out in a homogeneous solution, it would be of significance to develop the successive homogeneous assay system in dynamic solution for rapid disease diagnosis and high-throughput bioanalysis . In homogeneous assay, the work electrodes generally have capability of DNA capture but lack signal amplification , restricting its sensitivity. Here, a flow-homogeneous sensing system was proposed to realize the successive assay of microRNA , a model biomarker. Ultrathin 2D metal-organic framework (MOF) nanozymes with thickness of about 1 nm were facilely prepared by ultrasonic approach. Due to the excellent enzyme-like activity and adsorption capacity towards single-strand DNA (ssDNA), MOF nanozymes adsorbed on electrode simultaneously played two roles of ssDNA collector and signal-amplifier. To adapt the recoverable electrode to on-line monitoring, duplex-specific nuclease-assisted circle reaction was conducted to produce the turn-on amplified signal. Flow injection device was employed to realize the recycling of electrodes and the successive microRNA assay. The assay strategy showed low limit of detection (0.12 pM, S/N = 3) for microRNA, excellent renewability and acceptable reliability for real sample assay. The established system exerts the advantages of DNA-based homogeneous electrochemical sensing strategy. This work would not only expand homogeneous electrochemical assay to successive bioassay, but also provide the possibility for practical application of homogeneous sensing strategy.
研究领域:
生物化学
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医学诊断
·
分析化学
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DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Jiaying Zhao,Congjuan He,Weixuan Wu,Huisi Yang,Jiangbo Dong,Li Wen,Zhikun Hu,Mei Yang,Changjun Hou,Danqun Huo
Abnormal expression of microRNAs is greatly associated with the occurrence of various cancer types, revealing great potential of microRNA as biomarkers for cancer diagnosis and prognosis. Herein, a MXene-MoS 2 heterostructure enhancing electrochemical biosensor coupled with catalytic hairpin assembly (CHA) amplification approach for label-free determination of microRNA-21 (miR-21) was successfully assembled. In particular, the unique micro-nano heterostructure with large specific area and favorable electroconductivity exhibited the ability of excellent confinement effect. Thus, rendered the MXene-MoS 2 heterostructure the ability to trigger more target recycling reaction, giving new vitality to the traditional CHA amplification method. Meanwhile, thionine (Thi) and gold nanoparticles (AuNPs) were anchoring at the surface of MXene-MoS 2 heterostructure, respectively, empowered the sensor the capability of capture probes fixation and miR-21 label-free determination. When numerous electronegative double-stranded DNA generated, the electron transfer was greatly hindered, resulting in signal decrease. Accordingly, the design denoted a broad dynamic range from 100 fM to 100 nM and a detection limit of about 26 fM, comparable or lower than previous reported methods for miR-21 detection. Furthermore, the sensing platform supplied satisfactory selectivity , reproducibility and stability towards the miR-21 detection. The real sample determination also showed a promising performance under clinical circumstance. Finally, from the clinical standpoint, the proposed biosensor is a considerable platform toward early disease detection and monitoring.
研究领域:
生物传感器
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电化学
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癌症生物学
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纳米技术
·
分子诊断
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DOI
PubMed
高影响因子
Bioengineered
·
2021
·
IF 3.269
Yute Sun,Zuoqiong Hou,Binlin Luo,Chujun Li,Jinfang Liu,Jianlan Liu,Jian Tang,Gang Yao
To identify how circular RNA circRNA_0082835 impacts melanoma cells and lymphatic metastasis to observe whether it exerts effects through its action mechanism of sponging microRNA miR-429. Clinical baseline information was collected, and clinical samples were used for detection on circRNA_0082835 and EZH2. The expression of circRNA_0082835, EZH2, and miR-429 was detected by quantitative real-time PCR (RT-qPCR). Cell proliferation was tested with cell counting kit-8 (CCK-8). Flow cytometry was applied to examination of cell cycle levels. Cell invasion and migration were observed by transwell and wound healing. The expression of Wnt/β-catenin pathway, cell cycle and epithelial–mesenchymal transition (EMT) marker proteins was analyzed by western blot. Dual-luciferase determined the binding of miR-429 and circ_0082835. As a result, the expression of circRNA_0082835 was increased and that of miR-429 was decreased with the increase in lymphatic metastasis level. CircRNA_0082835 expression was downregulated by circ_0082835 interference, upregulated by EZH2 interference and also downregulated after transfection of both shRNA-circ_0082835 and shRNA-EZH2. Inhibiting circ_0082835 and EZH2 suppressed the proliferation, invasion and migration, regulated the cell cycle levels, inhibited Wnt/β-catenin and attenuated EMT in melanoma cells. Inhibition of circ_0082835 and/or EZH2 elevated miR-429 expression. The binding among miR-429 and circ_0082835 was verified. MiR-429 inhibitor reversed the effect of circ_0082835 interference while having no significant impact on EZH2. In conclusion, circRNA_0082835 sponges miR-429 to affect the anti-tumor effect of miR-429 in primary melanoma and lymphatic metastasis.
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2026
·Vol. 9
·期 31
·14775-14788
·
IF 5.8
Lin Zhuoran,Wu Wenjing,Zhao Hang,Jiang Yao,Xi Dongmei
Cancer phototherapies, such as photodynamic therapy (PDT)
and photothermal
therapy (PTT), are often limited by poor tumor accumulation, insufficient
penetration, and lack of real-time adaptability to the tumor microenvironment.
To address these challenges, we developed an intelligent nanoscale
theranostic nanoplatform, TDN HM -Au@IR780, based on a DNA
tetrahedron (TDN) functionalized with gold nanoparticles (AuNPs) and
the near-infrared dye IR780. The platform is further equipped with
MUC1-targeting aptamers and catalytic hairpin assembly (CHA) circuits
responsive to the tumor biomarker miRNA-21. Upon encountering miRNA-21
in the tumor microenvironment, the nanoparticles undergo in situ aggregation
via CHA, dynamically switching from small, penetrative particles to
larger aggregates that enhance retention. Under 808 nm laser irradiation,
the aggregated system exhibits strong plasmonic coupling, achieving
a high photothermal conversion efficiency of 45.8% and significantly
amplified singlet oxygen generation. In vitro and in vivo studies
using 4T1 breast cancer models demonstrate effective tumor-targeted
accumulation, excellent biocompatibility, and potent synergistic photothermal/photodynamic
effects. This nanoscale, dynamically responsive strategy is particularly
well-suited for image-guided precision phototherapy of breast cancer,
offering a promising avenue for nanomedicine-based oncology.
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DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-7
·
IF 5.479
Wang Jiaxing,Zhang Linlin,Lu Liping,Kang Tianfang
An electrochemiluminescence (ECL) based biosensor is described for determination of microRNAs in the A549 cell line. Firstly, graphene oxide (GO) is dripped onto a glassy carbon electrode surface to form an interface to which one end of the capture probe (with a stem-loop structure) can be anchored through π-interaction via dangling unpaired bases. The other end of the capture probe is directed away from the GO surface to make it stand upright. Target microRNAs can open the hairpin structure to form a double-stranded DNA-RNA structure. Two auxiliary probes, generating a hybridization chain reaction, are used to elongate the DNA duplex. Finally, doxorubicin-modified cadmium telluride quantum dot nanoparticles (Dox-CdTe QD) are intercalated into the base pairs of the hybrid duplexes to act as signalling molecules. The ECL signal of the Dox-CdTe QD increases proportionally with the concentration of microRNAs, specifically for microRNA-21. The assay covers a wide linear range (1 fM to 0.1 nM), has a low detection limit for microRNA-21 (1 fM), and is selective, reproducible, and stable.
An enzyme-free amplification electrochemiluminescent assay is described to quantitative detection of microRNA in the A549 cell line. Graphene oxide was used to immobilize capture probes obviating the special modification. Doxorubicin-modified cadmium telluride quantum dot nanoparticles are intercalated into the base pairs of the hybrid duplexes to act as signalling molecules.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）