JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2018
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IF 2.297
Xia Lv,Zhongxiao Cong,Zhihao Liu,Xiaodong Ma,Ming Xu,Yan Tian,Xinyi Zhang,Bingqing Xu,Jianbin Zhang,Zeyao Tang
Topical application of trans -resveratrol was limited due to its insolubility and instability problems. In this work, three kinds of essential oil based microemulsions containing rose oil (RO-ME), jasmine oil (JO-ME) and lemon oil (LO-ME) were prepared, constituted of Cremophor EL/1,2-propanediol/essential oils (47: 23: 30, w/w). All the three microemulsions could significantly improve the solubility and photostability of trans -resveratrol. Moreover, they could sustain the release of trans -resveratrol. Antioxidant activity study demonstrated that trans -resveratrol loaded microemulsions exhibited higher scavenging activities than both trans -resveratrol and blank microemulsions. The cell experiments demonstrated that RO-ME and JO-ME had lower cytotoxicity on HaCaT cells than LO-ME. The trans -resveratrol loaded RO-ME and JO-ME could significantly protect the cells from UVB damage. Finally, the in vitro skin permeation study exhibited that the microemulsions could enhance the skin permeation of trans -resveratrol in comparison to the aqueous solution. In summary, the essential oil based microemulsions could improve the solubility, photostability, antioxidant activity and UVB photoprotection of trans -resveratrol, which will be beneficial to its topical application.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2022
·
IF 3.119
Mengyang Guo,Ye Jiang,Yonghong Li,Yanyu Wei,Hanxi Li
The isobaric vapor–liquid equilibria (VLE) of two binary mixtures (adiponitrile + 2-methylglutaronitrile and adiponitrile + trans-3-pentenenitrile) at 5 and 10 kPa were measured using a modified Rose–Williams still. The thermodynamic consistency of the VLE data was tested by the Wisniak method and the Fredenslund method. The isobaric VLE data were correlated with four activity coefficient models of Margules, van Laar, Wilson, and NRTL, and the parameters of the models were obtained by the ordinary least-squares method. The isobaric VLE data were compared with the original UNIFAC predictive model. Furthermore, the excess Gibbs free energy of studied mixtures was determined. The RMST and RMSy1 were determined to evaluate the regression results. The results show that the Wilson model has a better correlation effect than other models.
研究领域:
化学工程
·
热力学
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Advanced Science
·
2025
·
IF 14.1
Shuai Wu,Yincheng Liu,Tianyu Zeng,Tianci Zhou,Yanting Sun,Ying Deng,Juan Zhang,Genxi Li,Yongmei Yin
Continued development of clustered regularly interspaced short palindromic repeats (CRISPR)-powered biosensing system on the electrochemical interface is vital for accurate and timely diagnosis in clinical practice. Herein, an electrochemical biosensor based on manganese metal-organic frameworks (MOFs)-enhanced CRISPR (MME-CRISPR) is proposed that enables the efficient detection of circulating tumor DNA (ctDNA). In this design, customized enzyme stimulants (Mn 2+ ) are co-assembled with Cas12a/crRNA to form enzyme-MOF composites, which can be released quickly under mild conditions. The MOFs-induced proximity effect can continuously provide adequate Mn 2+ to sufficiently interact with Cas12a/crRNA during the release process, enhancing the trans-cleavage activity of complex available for biosensor construction. The MOFs-based enzyme biocomposites also afford efficient protection against various external stimulus. It is demonstrated that the developed biosensor can achieve ultrasensitive detection of epidermal growth factor receptor L858R mutation in ctDNA with a low detection limit of 0.28 f m without pre-amplification. Furthermore, the engineered mismatch crRNA enables the biosensor based on MME-CRISPR to detect single nucleotide variant with a high signal-to-noise ratio. More importantly, it has been successfully used to detect the targets in clinical practice, requiring low-dose samples and a short time. This strategy is believed to shed new light on the applications of cancer diagnosis, treatment, and surveillance.
研究领域:
生物医学工程
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分子诊断学
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纳米医学
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FOOD CHEMISTRY
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2018
·
IF 4.946
Qin Zhao,Dan-Qi Cheng,Ming Tao,Wen-Jing Ning,Yong-Jiao Yang,Kai-You Meng,Yong Mei,Yu-Qi Feng
In the present study, a rapid and effective method based on alendronate sodium grafted mesoporous magnetic nanoparticle (Fe 3 O 4 @ANDS) extraction for the determination of trans-resveratrol (TRA) in peanut oils was developed by coupling with HPLC-UV detection. The Fe 3 O 4 @ANDS was prepared via Lewis acid/base interaction which was simply carried out in mild aqueous condition without the using of organic solvent. The resultant Fe 3 O 4 @ANDS encompassed amino group on its surface, and it was employed as magnetic solid-phase extraction adsorbent for purification and enrichment of TRA from peanut oils through hydrogen bond interaction. Under the optimized conditions, the whole pretreatment process could be accomplished within 10 min without time-consuming concentrated and reconstituted process. The linearity range of the proposed method was 1–10,000 ng/g with satisfactory correlation coefficient (R 2 ) of 0.9992. The recoveries in spiked oil samples were in the range of 78.6–118.9% with the RSDs less than 3.3% (intra-day) and 15.2% (inter-day). The limit of detection for TRA in peanut oils was 0.3 ng/g which was comparative to the reported methods by using LC-MS/MS detection. Finally, the established method was successfully applied to the analysis of TRA in several peanut oils with different brands from local market as well as other kinds of vegetable oils.
研究领域:
分析化学
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食品科学
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Materials Today Advances
·
2022
·
IF 9.918
Siyong Gao,Yuxin Tang,Wei Sun,Zhixin Liu,Tianyu Zhao,Xiang Li,Tianlu Wang,Guiqing Liao,Tao Xu,Guangsen Zheng,Yujie Liang
Schwann cells (SCs)-based nerve guidance conduits (NGCs) is a promising strategy for repairing long-gap peripheral nerve injury (PNI). But the number of SCs is limited as terminally differentiated cells. Matrix stiffness is able to direct cell fate of stem cells. And nerve-special stiffness (NSS) may contribute to stem cells converting into SCs. However, the potential mechanisms remain uncertain. The present study aimed to elucidate whether the given NSS was able to promote mesenchymal stem cells (MSCs) trans -differentiating into Schwann cell-like cells (SCLCs), facilitating cell-based NGCs repairing PNI. Gelatin methacryloylate (GelMA) hydrogels with different stiffness were manufactured, and their roles in the trans -differentiation of MSCs into SCLCs were investigated in vitro study. The most favorable stiffness (0.9–2.9 kPa) for trans -differentiation and neurotrophic factors expression was confirmed by western blot and immunofluorescence assays. The reason might lie in softer stiffness stretched cell morphology, leading to up-regulation of PIEZO1 and then activating YAP nuclear translocation. As for in vivo study, multi-channel NGCs containing SCLCs were fabricated with extrusion-based bioprinting and then filled a 5 mm gap in sciatic nerve defect of SD rats. It turned out that GelMA-made NGCs with stiffness of 2.9 kPa achieved promising neurogenerative capacity, not only promoting recovering sensory and motor functions, but also improving myelinated nerve fiber regeneration. Our findings demonstrated that 0.9–2.9 kPa might be the desired mechanical strength inducing trans -differentiation into SCLCs via PIEZO1/YAP axis, and the 3D-printed GelMA NGCs combined with SCLCs could be a potential candidate for long-gap peripheral nerve injury.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2023
·
IF 9.9
Shanshan Ma,Hejun Li,Qiyue Huang,Jie Fei
The development of interfacial engineering was crucial for achieving the industrialization of high-performance carbon fiber/phenolic composites. In this study, establishing scalable interpenetrating networks (cellulose nanofiber-zeolitic imidazolate frameworks-8/aramid nanofiber-boron nitride) on the fiber/matrix interphase, was in favor of realizing precise repairation of interfacial defects , further regulating thermal conductivity , mechanical and tribological properties of the composites. Based on the physical and chemical bridging-effects arising from above spider-web networks, the flexural strength and modulus of modified sample were 74.69 MPa and 6.22 GPa, showing an increase of 135.99% and 56.68%, respectively. Meanwhile, this trans-scale spider-web structure acted as a micron skeleton-nano unit continuous thermal conductive network, significantly reduced phonon scattering and displayed a 258.33% enhancement in the thermal management capability of modified sample. This study reveals key design principles of trans-scale interfacial structure to dynamicly regulate performances and meet service requirements of next-generation carbon fiber/phenolic composites.
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FEBS Open Bio
·
2023
·
IF 2.6
Yu Zhu,Longmei Wang,Rui Liu,Xiurong Ding,Song Yin,Yuankun Chen,Chuanlong Zhu,Zheng Wang,Wenting Li
Sepsis-induced acute kidney injury (SI-AKI) causes renal dysfunction and has a high mortality rate. Protein arginine methyltransferase-1 (PRMT1) is a key regulator of renal insufficiency. In this study, we explored the potential involvement of PRMT1 in SI-AKI. A murine model of SI-AKI was induced by cecal ligation and perforation (CLP). The expression and localization of PRMT1 and molecules involved in the TGF-β1/Smad3 and IL-6/STAT3 signaling pathways were detected in mouse kidney tissues by western blot, immunofluorescence, and immunohistochemistry. The association of PRMT1 with downstream molecules of the TGF-β1/Smad3 and IL-6/STAT3 signaling pathways were further verified in vitro in mouse renal tubular epithelial cells (mRTECs). CLP caused epithelial-mesenchymal transition (EMT), apoptosis, and inflammation in renal tissues, and this was alleviated by inhibition of PRMT1. Inhibition of PRMT1 in SI-AKI mice decreased the expression of TGF-β1 and phosphorylation of Smad3 in the renal cortex, and downregulated the expression of sIL-6R and phosphorylation of STAT3 in the medulla. Knockdown of PRMT1 in mRTECs restricted the expression of Cox-2, E-cad, Pro-caspase3 and p-Smad3 (involved in the TGF-β1-mediated signaling pathway), and blocked IL-6/sIL-6R, inducing the expression of Cox-2 and p-STAT3. In conclusion, our findings suggest inhibition of PRMT1 mitigates SI-AKI by inactivating the TGF-β1/Smad3 pathway in the cortex and IL-6/STAT3 pathway in the medulla. Our findings may aid in the identification of potential therapeutic target molecules for SI-AKI.
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JOURNAL OF MOLECULAR LIQUIDS
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2018
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IF 4.513
Huanxin Li,Xiaoqiang Gao,Yonghui Dou,Li Xu,Guoji Liu
The ternary systems of {(1-butanol or 1-pentanol) + (−)-trans-Cyclohexane-1,2-diamine + water} at (298.2–318.2) K under 100.02 kPa in terms of liquid-liquid equilibrium were investigated. The solubility data of each system were obtained by using cloud point method. For evaluation of extraction ability of the solvents used in this work, the distribution coefficients and separation factors were calculated and 1-pentanol was demonstrated to be very suitable. Besides, Othmer-Tobias and Hand correlation equations were applied to check the consistency of the tie-line data. Furthermore, NRTL and UNIQUAC models were used to correlate the experimental data with all the RMSD values less than 2.57%.
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RSC Advances
·
2018
·Vol. 8
·期 23
·12752-12759
·
IF 2.936
HuaQing Liang,QiHua Zhou,YongJiang Long,WanChu Wei,Shuo Feng,GuoDong Liang,FangMing Zhu
Herein, a novel amphiphilic diblock copolymer consisting of isotactic polystyrene (iPS) and 1,4-trans-polybutadiene-graft-poly(ethylene oxide) (1,4-trans-PBD-g-PEO), iPS-b-(1,4-trans-PBD-g-PEO), was synthesized by the combination of living coordination copolymerization and graft copolymerization. iPS-b-1,4-trans-PBD was firstly synthesized via sequential monomer addition in the presence of 1,4-dithiabutandiyl-2,2′-bis(6-cumenyl-4-methylphenoxy) titanium dichloride (complex 1) activated by triisobutyl aluminum modified methylaluminoxane (MMAO). Moreover, hydroboration of double bonds in the 1,4-trans-PBD blocks were performed with 9-borabicyclo[3.3.1]nonane (9-BBN) and subsequent oxidation by NaOH/H2O2 to form hydroxyls. Consequently, PEO was grafted into the hydroxylated 1,4-trans-PBD block in terms of ring-opening polymerization of ethylene oxide with potassium/naphthalide as initiatior. We also described solvent-evaporation-induced self-assembly of iPS-b-1,4-trans-PBD in n-dodecane and iPS-b-1,4-trans-PBD-g-PEO in aqueous solution, which were selective solvent for 1,4-trans-PBD and for 1,4-trans-PBD-g-PEO blocks, respectively. In these cases, tetrahydrofuran (THF) was used as good and volatile solvent. These resultant iPS-containing diblock copolymers could self-assemble into spherical nano-micelles with an iPS core as amorphous agglomeration or a very low degree of crystallinity resulting from slow crystallization rate and nanoconfinement. In addition, after isothermal crystallization of iPS in the micellar cores self-assembled in n-dodecane at 120 °C for 3 hours, the micellar morphology changed from sphere-like to platelet-like. It was believed that isothermal crystallization of iPS induced the deformation of the micelles.
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American Journal of Translational Research
·
2020
·
IF 3.375
Runzi Gao,Wenhui Ji,Tianwei Xia,Yanxing Fan,Wei Wei,Le Shi,Jinzhu Liu,Chao Zhang,Lezhen Xue,Jirong Shen
Osteonecrosis of the femoral head (ONFH) is a common disorder that may be idiopathic, caused by trauma, or associated with alcohol or glucocorticoid use. The goals of early treatment include delaying or avoiding hip replacement, but there are no effective treatments for early-stage disease. The aim of the present study was to evaluate the effects of treatment with 3D-printed porous titanium alloy scaffold combined with daily intraperitoneal trans-cinnamaldehyde (TCA) in a dog model of ONFH. Four weeks after creation of the ONFH model, MRI examination of the femoral head showed the characteristic “double line sign” of ONFH, verifying the validity of our model. After another 12 weeks, femoral head specimens were harvested and examined by gross inspection; micro-computed tomography; histologic staining (hematoxylin and eosin; Masson); immunohistochemical analysis and quantitative real-time polymerase chain reaction analysis. Gross inspection of the femoral head in untreated ONFH animals at 16 weeks after model creation showed pale, exfoliating articular cartilage and disordered trabecular bone. Treatment with 3D-printed titanium alloy porous scaffold combined with TCA ameliorated the pathologic ONFH changes and significantly reduced inmature bone tissue as well as imature collagen in the femoral head, as shown by Masson staining. This treatment also increased VEGF, BMP2, β-catenin, b-FGF, and RUNX2 expression and decreased PPARγ expression, compared with untreated ONFH. In conclusion, 3D-printed titanium alloy porous scaffold combined with TCA can effectively improve ONFH, which may be related to local repair. This provides the theoretical basis for a new treatment strategy for ONFH.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Qiuli OuYang,MingChen Yang,Yonghua Zhang,Lu Li,Xiaoli Tan,Nengguo Tao
Huge postharvest citrus infections are produced by Penicillium digitatum . The antifungal activity of sodium trans -2-hexenylate (STH) against the P. digitatum growth and possible mechanism were investigated. The minimal inhibitory and fungicidal concentrations of STH against P. digitatum were 0.4 and 0.8 g L –1 , respectively. STH decreased the green mold incidence of Ponkan fruit without negatively impacting fruit quality. STH altered and distorted the external appearance of P. digitatum mycelia rather than the cell wall. Correspondingly, STH decreased the lipid and ergosterol levels while degrading the cell membrane integrity. Meanwhile, STH affected mitochondrial function by lowering the mitochondria membrane potential (MMP) and ATP levels, as well as malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) activities. Furthermore, MMP damage occurred earlier than that of cell membrane damage. Our current study showed that STH may be an acceptable substitute to chemical fungicide in postharvest disease control in citrus fruit and its underlying inhibitory mechanism is attributed to its ability to impair the mitochondrial function of P. digitatum .
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CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Yujie Zhang,Xiaojiang Zhang,Lianxi Lv,Sheng Gao,Xiang Li,Ruochen Wang,Pengqian Wang,Feiyu Shi,Junjun She,Ya Wang
To achieve effective long-term synergistic treatment of inflammatory bowel disease (IBD) with probiotics, we developed a versatile inulin/trans-ferulic acid/silk sericin nanoparticles-nourished probiotic complex. Inulin/TFA/SS nanoparticles were fabricated by inulin, trans-ferulic acid (TFA), and silk sericin (SS), and then loaded onto the surface of poly- l -lysine (PLL) and poly-glutamic acid (PGA)-coated Bifidobacterium longum (BL) to obtain BL@PLL-PGA-Inulin/TFA/SS NPs (BL@PP-NPs). This design simultaneously endowed the complex with excellent gastrointestinal resistance, antioxidant, and anti-inflammation abilities. Moreover, the inulin in the nanoparticles acts as a prebiotic, promoting the Bifidobacterium's rapid proliferation to exert effects within a short period. Compared with uncoated BL, BL@PP-NPs exhibited excellent gastric acid tolerance and up to 31.32-fold colonic colonization, and the ROS scavenging and proliferative capacity were increased by 5.61- and 1.39-fold, respectively. In a mouse model of dextran sulfate sodium (DSS)- and trinitrobenzene sulfonic acid (TNBS)-induced colitis, the components of Inulin/TFA/SS NPs synergized with probiotics to efficiently treat IBD by attenuating oxidative stress, decreasing inflammation, repairing intestinal barrier, and promoting the rapid proliferation of probiotics to reverse gut microbial disorders. Collectively, food-grade BL@PP-NPs represent a novel approach to probiotic modification that offers an effective, safe, and synergistic therapy for IBD.
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