Nature Communications
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2022
·Vol. 13
·期 1
·1-8
·
IF 17.694
Chen Liang,Deng Shiqing,Liu Hui,Wu Jie,Qi He,Chen Jun
Next-generation advanced high/pulsed power capacitors rely heavily on dielectric ceramics with high energy storage performance. However, thus far, the huge challenge of realizing ultrahigh recoverable energy storage density ( W rec ) accompanied by ultrahigh efficiency ( η ) still existed and has become a key bottleneck restricting the development of dielectric materials in cutting-edge energy storage applications. Here, we propose a high-entropy strategy to design “local polymorphic distortion” including rhombohedral-orthorhombic-tetragonal-cubic multiphase nanoclusters and random oxygen octahedral tilt, resulting in ultrasmall polar nanoregions, an enhanced breakdown electric field, and delayed polarization saturation. A giant W rec ~10.06 J cm −3 is realized in lead-free relaxor ferroelectrics, especially with an ultrahigh η ~90.8%, showing breakthrough progress in the comprehensive energy storage performance for lead-free bulk ceramics. This work opens up an effective avenue to design dielectric materials with ultrahigh comprehensive energy storage performance to meet the demanding requirements of advanced energy storage applications.
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Nature Protocols
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2022
·Vol. 17
·期 10
·2240-2274
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IF 17.021
Zhao Xiao,Zhao Ruifang,Nie Guangjun
Here we present a protocol for the construction and use of two types of nanocarrier based on bacterial membrane materials for cancer vaccine delivery. Cancer vaccines induce tumor regression through triggering the specific T-cell responses against tumor neoantigens, a process that can be enhanced by nanocarrier delivery. Inspired by the body’s natural immune defenses against bacterial invasion, we have developed two different types of nanocarrier based on bacterial membrane materials, which employ genetically engineered outer-membrane vesicles (OMVs), or hybrid membrane vesicles containing bacterial cytoplasmic membrane, respectively. The OMV-based nanocarriers can rapidly display different tumor antigens through the surface modified Plug-and-Display system, suitable for customized cancer vaccines when the tumor neoantigens can be identified. The hybrid membrane-based nanocarriers are prepared through fusion of the bacterial cytoplasmic membrane and the primary tumor cell membrane from surgically removed tumor tissues, possessing unique advantages as personalized cancer vaccines when the neoantigens are not readily available. Compared with chemically synthesized nanocarriers such as liposomes and polymer without intrinsic adjuvant properties, owing to the large amounts of pathogen-associated molecular patterns, the two nanocarriers can activate the antigen-presenting cells while delivering multiple antigens, thus inducing effective antigen presentation and robust adaptive immune activation. Excluding bacterial culture and tumor tissue collection, the preparation of OMV- and hybrid membrane-based nanocarriers takes ~8 h and 10 h for tumor vaccine construction, respectively. We also detail how to use these nanocarriers to create cancer nanovaccines and evaluate their immunostimulatory and antitumor effects.
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MOLECULAR NEUROBIOLOGY
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2024
·
IF 4.6
Xu Yao,Liu Yongsheng,Wu Yan,Sun Jingshan,Lu Xiaocheng,Dai Kun,Zhang Yiting,Luo Chengliang,Zhang Jian
Early brain injury caused by subarachnoid hemorrhage (SAH) is associated with inflammatory response and ferroptosis. Curcumin alleviates neuroinflammation and oxidative stress by as yet unknown neuroprotective mechanisms. The objective of this study was to investigate the impact of curcumin on neuronal ferroptosis and microglia-induced neuroinflammation following SAH. By examining Nrf2/HO-1 expression levels and ferroptosis biomarkers expression both in vitro and in vivo, it was demonstrated that curcumin effectively suppressed ferroptosis in neurons after SAH through modulation of the Nrf2/HO-1 signaling pathway. Furthermore, by analyzing the expression levels of Nrf2, HO-1, p-p65, and inflammation-related genes, it was confirmed that curcumin could prevent the upregulation of pro-inflammatory factors following SAH by regulating the Nrf2/HO-1/NF-κB signaling pathway in microglia. The ability of curcumin to reduce neuronal damage and cerebral edemas after SAH in mice was validated using TUNEL staining, Nissl staining, and measurement of brain tissue water content. Additionally, through implementation of the modified Garcia test, open field test, and Y-maze test, it was established that curcumin ameliorated neurobehavioral impairments in mice post-SAH. Taken together, these data suggest that curcumin may offer a promising therapeutic approach for improving outcomes following SAH by concurrently attenuating neuronal ferroptosis and reducing neuroinflammation. Graphical Abstract
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Clinical Oral Investigations
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2024
·Vol. 28
·期 10
·1-14
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IF 3.1
Liu Runze,Liu Pei,Luo Yi,Fan Wei,Fan Bing
Objectives The mannose phosphotransferase system (Man-PTS) plays crucial roles in the adaptive metabolic activity of Enterococcus faecalis ( E. faecalis ) in adverse environments. The aim of this study was to evaluate the role of Man-PTS in the alkaline resistance of E. faecalis against calcium hydroxide (CH) and the effect of metformin (Met) on the alkaline resistance of E. faecalis to CH. Materials and methods The regulatory role of Man-PTS EII in the alkaline resistance of E. faecalis was firstly investigated using a wild-type highly alkaline-resistant E. faecalis XS 003, standard ATCC 29212 and Man-PTS EIID gene deficient (△ mptD ) and overexpressing (+ mptD ) strains of E. faecalis . RNA sequencing of Met-treated E. faecalis was performed to further validate the effect of Met on Man-PTS. The effect of Met on CH resistance of E. faecalis was verified by evaluating the survival, membrane potential and permeability, intracellular pH and ATP, and the expression of Man-PTS EII and membrane transporter-related genes of E. faecalis . The effect of Met on the ability of CH to remove E. faecalis biofilm on the dentin surface was also tested. The in vivo therapeutic effect of Met plus CH (CHM) was further investigated in a rat apical periodontitis model induced by E. faecalis XS 003. Results Man-PTS EII significantly promoted the survival ability of E. faecalis in CH and enhanced its resistance to CH. The inhibition of Man-PTS EII by Met resulted in reduced alkaline resistance of E. faecalis in the presence of CH, while also enhancing the antimicrobial properties of CH against E. faecalis biofilm on dentin. Additionally, Met plus CH showed the synergistically promoted intra-canal E. faecalis infection control and healing of periapical lesion in rats. Conclusions Met could significantly reduce the alkaline resistance of E. faecalis against CH through the modulation of Man-PTS EII, and improved the antibacterial effect of CH against E. faecalis infection both in vitro and in vivo. Clinical relevance Met could significantly enhance the ability of CH to control E. faecalis infection through reducing the alkaline resistance of E. faecalis .
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NEUROCHEMICAL RESEARCH
·
2018
·Vol. 43
·期 5
·1058-1066
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IF 2.772
Nong Xiaolian,Lan Yuyan
Reactive astrocyte-mediated neuroinflammatory responses in the spinal dorsal horn have been reported to play a pivotal role in pathological pain. Chronic constriction injury (CCI) enhances the activation of nuclear factor kappa B (NF-κB), which is involved in neuropathic pain (NP). Picroside II (PII), a major active component of Picrorhiza scrophulariiflora, has been investigated for its anti-oxidative, anti-inflammatory, and anti-apoptotic activities. Here, we explored the analgesic effects of PII on a model of CCI-induced NP and investigated the levels of the GFAP protein and the mRNA and protein levels of pro-inflammatory cytokines in the spinal cord, including interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). CCI significantly induced mechanical allodynia and thermal hyperalgesia. Intraperitoneal administration of PII remarkably reversed the CCI-induced mechanical allodynia and thermal hyperalgesia and reduced the mRNA and protein levels of IL-1β, IL-6, and TNF-α in the spinal cord. Additionally, according to the in vitro data, the PII treatment inhibited LPS-induced increases in the mRNA and protein levels of IL-1β, IL-6, and TNF-α and suppressed the NF-κB pathway by inhibiting the phosphorylation of NF-κB/p65 and the degradation of inhibitor of NF-κB (IκB) in astrocytes without toxicity to astrocytes. Overall, the analgesic effect of PII correlated with the inhibition of spinal reactive astrocyte-mediated neuroinflammation through the NF-κB pathway in rats with NP.
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MICROCHIMICA ACTA
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2019
·Vol. 186
·期 6
·1-8
·
IF 5.479
Shi Tian,Fu Hailu,Tan Liju,Wang Jiangtao
A fluorescent nanoprobe consisting of CdTe quantum dots (QDs) and coated with molecularly imprinted layers was prepared and successfully applied to the determination of norfloxacin (NOR) in seawater and wastewater samples. The 3-mercaptopropionic acid capped QDs were prepared and then covered with a protective silica shell. A molecularly imprinted layer was finally synthesized around the silanized QDs using 3-aminopropyltriethoxysilane as functional monomer and norfloxacin as the template. Compared with the non-imprinted polymer, the fluorescence of the nanoprobe with imprinted polymer (measured at excitation/emission wavelengths of 300/596 nm) is strongly reduced in the presence of NOR, and the imprinting factor is 8.8. Under the optimal experimental conditions, the detection limit of the nanoprobe is 0.18 μM, and response is linear between 0.5 - 28 μM of NOR. The relative standard deviation of the detection of NOR is <7.2%. In order to evaluate the practicality of the probe, wastewater and seawater samples spiked with norfloxacin were analyzed by this method, and recoveries ranged from 96.2 - 106.0%.
Schematic presentation and fluorescence spectrum of fluorescent nanoprobe with selectivity for norfloxacin (NOR). CdTe quantum dots (QDs) are used as fluorescent carriers, 3-aminopropyltriethoxysilane (APTES) as template molecules, tetraethyl orthosilicate (TEOS) as crosslinking agent, and aqueous ammonia as initiator.
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Microfluidics and Nanofluidics
·
2015
·Vol. 19
·期 2
·447-456
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IF 2.528
He Yong,Qiu Jingjiang,Fu Jianzhong,Zhang Jiong,Ren Yina,Liu An
This study demonstrated how to quickly and effectively print two-dimensional (2D) and three-dimensional (3D) microfluidic chips with a low-cost 3D sugar printer. The sugar printer was modified from a desktop 3D printer by redesigning the extruder, so the melting sugar could be extruded with pneumatic driving. Sacrificial sugar lines were first printed on a base layer followed by casting polydimethylsiloxane (PDMS) onto the layer and repeating. Microchannels were then printed in the PDMS solvent, microfluidic chips dropped into hot water to dissolve the sugar lines after the PDMS was solidified, and the microfluidic chips did not need further sealing. Different types of sugar utilized for printing material were studied with results indicating that maltitol exhibited a stable flow property compared with other sugars such as caramel or sucrose. Low cost is a significant advantage of this type of sugar printer as the machine may be purchased for only approximately $800. Additionally, as demonstrated in this study, the printed 3D microfluidic chip is a useful tool utilized for cell culture, thus proving the 3D printer is a powerful tool for medical/biological research.
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INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TECHNOLOGY
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2026
·
IF 3.7
Cui Xiangxiang,Wei Tianlu,Chen Xingqiang,Fang Yinghui,Yang Hai,Zhu Xingfei
Thin‑walled hard‑brittle curved‑surface quartz crucibles are critical high‑temperature components for Czochralski monocrystalline silicon growth. Inner‑wall defects such as chipping pits and black spots cause crucible rejection, whereas conventional planar chemical‑mechanical polishing (CMP) fails for curved‑surface defect remediation. Herein, a rare‑earth‑based photocatalysis‑assisted eco‑friendly CMP slurry was developed. Taguchi L25 (5 5 ) orthogonal tests were performed to optimize slurry formulation and processing parameters using areal roughness Sa, signal‑to‑noise ratio and mean roughness as criteria. White‑light interferometry and X‑ray photoelectron spectroscopy (XPS) were employed to characterize surface topography and interfacial chemistry, and crystal‑pulling trials evaluated the service performance of repaired crucibles. The optimal slurry contains 10 vol.% H 2 O 2 , 8 wt.% CeO 2 , pH 9.5, 0.8 wt.% CMC‑Na, 3 wt.% EDTA‑5Na, 3 wt.% TiO 2 and 1 wt.% lanthanum carbonate modifier. Optimal parameters are: normal load 12 N, polishing duration 60 min, illumination intensity 4000 Lux, rotational speed 3000 r/min, slurry flow rate 80 mL/min. Applying this integrated process to 32‑inch spent crucibles eliminates typical inner‑wall defects and yields ultra‑smooth surfaces with Sa 0.724–0.844 nm and seamless substrate‑polished‑zone transition. Comparative pulling tests reveal no statistically significant differences in key crystal‑growth metrics between repaired and brand‑new crucibles. XPS demonstrates that Ce‑based abrasives govern interfacial reactions: photocatalytically generated hydroxyl radicals produce silicate softening layers and surface Si‑OH groups, which condense with abrasive‑bound Ce‑OH to form Ce‑O‑Si bonds removed via mechanical shearing. CMC‑Na suppresses scratches and mitigates heavy‑load damage. This slurry‑photocatalysis synergy enables high‑value‑added remanufacturing of spent quartz crucibles and benefits material‑loss reduction for the photovoltaic silicon chain.
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JOURNAL OF MATERIALS SCIENCE
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2026
·Vol. 61
·期 42
·33005-33018
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IF 4.4
Chang Tong,Shi Yufen,Wu Pingyu,Xie Wanyu,Hao Ruipeng,Gao Xuwen,Lu Wenbo
The development of high-performance sensing materials for the simultaneous detection of multiple environmental pollutants remains a critical challenge in analytical chemistry. Herein, we report the synthesis of bimetallic phosphide (NiCoP) nanoflowers via a facile two-step hydrothermal and phosphorization strategy, and their application as an efficient electrochemical sensor for the simultaneous determination of hydroquinone (HQ), catechol (CC), and acetaminophen (AP). The hierarchical three-dimensional nanoflower architecture, constructed from interconnected nanosheets, endows the material with a large specific surface area and abundant accessible active sites. The NiCoP-modified glassy carbon electrode (NiCoP/GCE) exhibits good electrocatalytic activity toward the oxidation of HQ, CC, and AP. Under optimized conditions, the sensor displays wide linear dynamic ranges (10–100000 μM for HQ, 25–19000 μM for CC, and 25–15000 μM for AP) and low detection limits (5.23 μM, 10.24 μM, and 9.53 μM for HQ, CC, and AP, respectively). Furthermore, the sensor demonstrates good selectivity and stability. The practical applicability of the sensor was successfully validated by quantifying the three analytes in real water samples with satisfactory recoveries. This work highlights the potential of bimetallic phosphides as robust sensing materials for environmental monitoring
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Nature Synthesis
·
2026
·
IF 22.6
Hu Rong,Chen Jinli,Cai Lebin,Liu Haojie,Rao Yi,Zeng Xiaoxiao,Lian Junyi,Lin Cheng,Wang Bin,Yang Shengchun,Wan Jun,Xu Weilin,Xia Bao Yu,Yao Yonggang
The design of high-performance alloy catalysts is constrained by thermodynamic immiscibility among constituent elements (for example, W–Bi). High-entropy designs can overcome immiscibility by incorporating multiple elements, but active sites are often diluted and structure–property relationships are obscured. Here we report a compositionally minimalist seed-confined alloying strategy that uses a single thermodynamically selected seed, rather than multiple additional alloying elements, together with pathway-controlled alloy formation. Pt was selected as a representative seed because favourable Pt–W and Pt–Bi interactions improve ternary alloying accessibility while providing active sites for methanol oxidation; pre-anchoring creates dispersed incorporation centres, promotes multi-element association and suppresses competing phases. The resulting WBi–Pt@B/C catalyst comprises dispersed, robust nanoparticles with a diameter of ~3.6 nm and delivers a mass activity of 13.41 A per mg Pt in methanol oxidation, retaining 94.1% of its activity after 5,000 cycles. This strategy provides a practical route to the construction of compositionally simple nanoscale alloy catalysts with chemically complementary functions.
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Cancer Cell International
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2022
·Vol. 22
·期 1
·1-18
·
IF 6.429
Wang Shunxi,Chen Long,Liu Wanqian
Background Ferroptosis have been implicated in tumorigenesis, tumor progression, and chemo- and immuno-therapy in cirrhotic hepatocellular carcinoma (HCC), indicating its association with matrix stiffness and clinical benefit of targeting drugs or immune checkpoint inhibitor. Here, we postulated that increased matrix stiffness reduces ferroptosis and impairs tumor immunity by regulating the expression of ferroptosis- and immune-related genes in HCC, which might be a robust predictor of therapeutic efficacy. Methods Using publicly available tissue microarray datasets, liver cancer rat model, and clinical specimen, ferroptosis-related differential genes in HCV-infected cirrhotic HCC and its mechanical heterogeneous pattern of expression were screened and identified. Further investigation on the underlying mechanism of matrix stiffness-regulated ferroptosis and the expression of immune mediator were performed. Finally, threshold analysis of HCC cases with sorafenib treatment revealed the value of clinical applications of these potential predictors. Results STEAP3 was identified as the ferroptosis-related differential genes in HCV-infected cirrhotic HCC. Stiffer matrix decreased STEAP3 in the invasive front area of HCC and the liver cirrhotic tissue. Contrarily, softer matrix induced STEAP3 in the central area of HCC and the normal liver tissue. Immunological correlation of STEAP3 in cirrhotic HCC showed that STEAP3-mediated immune infiltration of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells and HCC prognosis, predicting to regulate immune infiltration. Overexpression of STEAP3 induced ferroptosis and inhibited the expression of immune mediator of PD-L2 on a stiff matrix. Especially, the ferroptosis- and immune-related gene predictive biomarker (FIGPB), including STEAP3 and PD-L2, predicts better clinical benefit of sorafenib in HCC patients. Conclusions This finding identifies matrix stiffness impairs ferroptosis and anti-tumor immunity by mediating STEAP3 and PD-L2. More importantly, coordinated with PD-L2, matrix stiffness-dependent STEAP3 could be applied as the independent predictors to favorable sorafenib response, and thus targeting it could be a potential diagnosis and treatment strategy for HCC.
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Journal of Orthopaedic Surgery and Research
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2022
·Vol. 17
·期 1
·1-8
·
IF 2.677
Zhang Bin-Fei,Song Wei,Wang Jun,Wen Peng-Fei,Zhang Yu-Min
Objectives The lung injury is often secondary to severe trauma. In the model of crush syndrome, there may be secondary lung injury. We hypothesize that high-mobility group box 1 (HMGB1), released from muscle tissue, mediates the apoptosis of alveolar epithelial cells (AEC) via HMGB1/Receptor of advanced glycation end-products (RAGE)/c-Jun N-terminal kinase (JNK) pathway. The study aimed to investigate how HMGB1 mediated the apoptosis of AEC in the rat model. Methods Seventy-five SD male rats were randomly divided into five groups: CS, CS + vehicle, CS + Ethyl pyruvate (EP), CS + FPS-ZM1 group, and CS + SP600125 groups. When the rats CS model were completed after 24 h, the rats were sacrificed. We collected the serum and the whole lung tissues. Inflammatory cytokines were measured in serum samples. Western blot and RT-qPCR were used to quantify the protein and mRNA. Lastly, apoptotic cells were detected by TUNEL. We used SPSS 25.0 for statistical analyses. Results Nine rats died during the experiments. Dead rats were excluded from further analysis. Compared to the CS group, levels of HMGB1 and inflammatory cytokines in serum were downregulated in CS + EP, CS + FPS-ZM1, and CS + SP600125 groups. Western blot and RT-qPCR analysis revealed a significant downregulation of HMGB1, RAGE, and phosphorylated-JNK in CS + EP, CS + FPS-ZM1, and CS + SP600125 groups, compared with the CS groups, excluding total-JNK mRNA. Apoptosis of AEC was used TUNEL to assess. We found the TUNEL-positive cells were downregulated in CS + EP, CS + FPS-ZM1, and CS + SP600125 groups. Conclusion The remote lung injury begins early after crush injuries. The HMGB1/RAGE/JNK signaling axis is an attractive target to abrogate the apoptosis of AEC after crush injuries.
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