Reaction Chemistry & Engineering
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2024
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IF 3.4
Yinghui Mu,Xin Ju,Cuiying Hu,Lishi Yan,Jiayi Tian,Su Ma,Liangzhi Li
Protein surface charge engineering has promising applications for a deeper understanding of the adsorption and action mechanisms between enzymes and substrates. The engineering modification of β-glucosidase (BGL) is conducive to greatly reducing the negative feedback inhibition in biomass resource utilization and improving the utilization efficiency. Herein, the mutants BGL-14 and BGL-1, which had the largest difference in surface ζ-potential (−12.5 mV and −4.6 mV) obtained by rational design in previous studies, were selected to explore their adsorption behaviors on cellulose and lignin using an adsorption isotherm model. The results showed that more negative charges on the enzyme surface facilitated adsorptive contact with substrates while repelling lignin to reduce competitive adsorption, and that the monolayer coverage might be the main mechanism for cellulose/lignin adsorption of enzyme. Secondly, commercial cellulases were added to the modified BGLs to form a “cocktail” of enzymes (BGL-C) to synergistically participate in the in situ saccharification of biomass pretreated with ionic liquids (ILs). The BGL-14-C synergistic hydrolysis system presented a strong conversion ability in low concentrations of ILs, and its catalytic biomass production of reducing sugars at 5% (v/v) 1-ethyl-3-ethyl-imidazolium diethylphosphate ([EEIM]DEP) reached a yield of 1.24 g L−1, which was 130% and 136% of that of WT-C and BGL-1-C, respectively. BGL-1-C, on the other hand, exhibited higher thermal stability, with the catalytic rate remaining at 51% when the temperature was increased from 50 °C to 70 °C in 1-ethyl-3-methyl-imidazolium diethylphosphate ([EMIM]DEP). Our study delves into the role of enzyme surface charge in reducing unproductive adsorption and increasing the probability of contact with the substrate, providing a theoretical basis for understanding the role of surface charge in mixed systems.
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高影响因子
FUEL
·
2024
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IF 6.7
Yuqi Tang,Wen Xu,Shi Liu,Xuemin Yan,Lu Lai,Weichu Yu,Yuanzhu Mi,Huan Li
A clover-shaped demulsifier (M−MEA) was synthesized via a simple two-step reaction, and its chemical structure was corroborated using 1 H NMR and FT-IR. The demulsification efficacy of M−MEA was investigated on two distinct water-in-oil emulsions. The bottle test results indicated that in a W/O emulsion with 30 % oil content, M−MEA achieved a demulsification efficiency (DE) of 100 % at a concentration of 300 mg/L, temperature of 60 °C, and settling time of 120 min. Similarly, in a W/O emulsion with 70 % oil content, the DE also reached 100 % at a concentration of 500 mg/L and a settling time of 90 min. Moreover, M−MEA exhibited remarkable demulsification performance at high salinity and across a wide pH range. Comparative analysis with other commercially available demulsifiers revealed that M−MEA offered high demulsification efficiency and yielded a clearly separated water phase. The study delved into the demulsification mechanism through assessments of dynamic interfacial tension (IFT), surface tension (ST), three-phase contact angle (TCA), and zeta potential . The findings demonstrated that M−MEA, acting as an effective demulsifier, swiftly migrated to the oil–water interface, displaced the natural surfactant , and destabilized the emulsion, thereby facilitating the oil–water separation of emulsions.
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Cell Reports Physical Science
·
2025
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IF 7.3
Yuting Xie,Hongyan Zhang,Partha Maity,Jinchen Qian,Gaopeng Xu,Hao Wang,Ting Chen,Kun Zhou,Hanlin Hu,Qingquan He,Jing Li,Yongwu Peng,Omar F. Mohammed,Jun Pan
Encapsulating perovskite nanocrystals within porous materials, particularly covalent organic frameworks (COFs), has emerged as a viable strategy for improving their resistance to water and light-induced degradation. However, previous studies have uncovered challenges arising from the intricate post-modification processes required for incorporating functional sites, which restricted the confinement growth of perovskite nanocrystals within COF pores. Herein, we synthesize a novel thiomethyl-functionalized COF (S-COF) by reacting 2,5-bis(3-(methylthio)propyloxy) terephthalate hydrazine (S-BMTH) with benzaldehyde,4,4′,4”-(1,3,5-triazine-2,4,6-triyl)tris- (TFPT). Subsequently, a highly stable composite, MAPbBr 3 @S-COF, is prepared by in situ growth of strongly confined MAPbBr 3 quantum dots (QDs) inside the S-COF pores. Interestingly, this composite demonstrates exceptional water stability for more than 1 year, attributed to the synergistic protection provided by the S-COF and Pb(OH)Br, which shield the QDs from degradation.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Li Xiang,Yuting Qin,Lei Li,Xianjing Xiang,Wenhui Zhang,Qiangqiang Jiao,Yaru Shao,Xinqiong Huang,Meichun Wu,Tianle Zhou,Yukang Lin,Yuping Chen
Liver fibrosis is a pathological condition marked by the excessive buildup of extracellular matrix primarily resulting from the transformation of quiescent hepatic stellate cells (HSCs) to myofibroblastic (MF) phenotype and their resultant over-expansion. Activated HSCs completely rely on their hyperactive mitochondria to supply the energy and biomass for their rapid proliferation and collagen secretion, so an intervention targeting their mitochondria can effectively restrict their pathological amplification and contribution to liver fibrosis. Here we tried sorafenib, a drug that plays anticancer roles by inducing the disruption and loss of mitochondrial functions, to reach an antifibrotic goal. And a complex micellar system, VA-PEG-PCL/TPGS (VPP/TPGS), was specifically designed and fabricated to encapsulate and deliver sorafenib selectively to activated HSCs to overcome its application limitations in bioavailability, toxicity and intracellular stay, and eventually maximize its induction of mitochondrial dysfunction and therapeutically antifibrotic efficacy. The prepared sorafenib complex micelles not only exhibited a suitable particle size, uniform morphology, and nice stability, but also performed excellently in the biosafety and HSCs-targetability in vitro and in vivo . In human active HSC cell lines, they markedly attenuated mitochondrial hyperactivity, induced apoptosis, and downregulated fibrosis markers as expected; while in a CCl 4 -induced murine model of hepatic fibrosis, they effectively restricted the expansion of MF-HSCs, reduced collagen deposition, and promoted the healing of liver damage, showing a good potential in fibrosis curation. Collectively, our VPP/TPGS complex micelles provide an ideal drug delivery platform that has the potential to revolutionize the treatment of liver fibrosis via addressing its cellular and metabolic underpinnings and thus improve patient outcomes.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Jinlong Zhang,Shiyu Fu,Chuanshuang Hu,Xi Yuan,Xuepei Zhou
Cellulose biocomposites have emerged as attractive alternatives to fossil-based plastics because of their excellent renewability and biodegradability; however, their water resistance and mechanical properties remain challenging. Herein, a cellulose- containing bioplastic with high a reinforcement content, water stability, and toughness is reported. Lignin-containing cellulose nanofibers (LCNF) were prepared by pretreating eucalyptus wood powder with a deep eutectic solvent and high-pressure homogenization. Then, the pre-synthesized ε-caprolactone oligomers were in-situ polymerized in LCNF. The interaction of LCNF with ε–caprolactone-oligomers in the LCNF-crosslinked polycaprolactone (LCNF-PCL) bioplastic resulted in excellent mechanical properties (tensile strength: 76.59 MPa; toughness: 9.82 MJ m −3 ). The LCNF-PCL bioplastic also demonstrated excellent water stability (wet tensile strength: 34.21 MPa; water absorption: <5 %), thermal stability, and UV protection. This approach may provide a potential method for utilizing lignocellulosic resources to develop environmentally friendly bioplastics with good toughness and water stability.
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高影响因子
Environmental Technology & Innovation
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2024
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IF 6.7
Shiyuan Liu,Qin Qiu,Hong Yang,Lai Zhou,Xueqiang Zhu
Polycyclic Aromatic Hydrocarbons (PAHs) are persistent organic pollutants known for their carcinogenic, teratogenic, and mutagenic properties. A considerable amount of PAHs has been released into groundwater. Persulfate-based advanced oxidation processes (PS-AOP) are commonly employed to remediate such contamination. However, traditional in-situ chemical oxidation (ISCO) faces challenges such as concentration rebound and tailing. To address these issues, sodium persulfate sustained release materials (PDS@SA) and ferrous sulfate sustained release materials (FeSO 4 @SA) were developed using stearic acid (SA) as a binder. Batch and column experiments were conducted to evaluate the efficacy of pyrene degradation through PDS@SA activated by FeSO 4 @SA. The batch experiments showed that the optimal mass ratio of FeSO 4 @SA to PDS@SA was 1:2, achieving degradation efficiencies over 95 % for pyrene with various concentrations (5, 10, 15, 20 mg⸱L −1 ) within 2 h. The column experiments showed that sustained release materials (FeSO 4 @SA and PDS@SA) with a particle size of 5 mm achieved an average removal rate of 89.3 % during days 7–15, while those with particle sizes of 7.5 mm and 10 mm achieved average removal rates of 68.5 % and 74.6 % during days 15–23, respectively. Additionally, electron paramagnetic resonance (EPR) analysis confirmed sulfate radicals SO 4 - ⸱ and hydroxyl radicals ⸱OH as the primary oxidative species during pyrene degradation. These findings suggested that the sustained activation of PDS by sustained release Fe 2+ represents a viable method for degrading PAHs in groundwater.
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APPLIED SURFACE SCIENCE
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2024
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IF 6.3
Song Ningbo,Yin Wanzhong,Xie Yu,Yao Jin
Using seawater for mineral flotation conserves fresh water resources and offers significant environmental and economic benefits. However, the adverse effect of seawater restricts its application in mineral flotation. The regulators can eliminate the adverse effect caused by seawater during flotation separation of lead and copper. In this paper, the flotation separation of galena and chalcopyrite in seawater was tested. Compared with those in deionized water, the lead grade of concentrate in seawater flotation declined from 50.10% to 48.90%. Meanwhile, the recovery of galena declined from 74.33% to 60.58% and the SI value declined from 1.565 to 1.324. With the addition of SHMP (sodium hexametaphosphate), SS (sodium silicate) and EDTA (ethylenediamine tetraacetic acid), the SI value increased from 1.324 to 1.923, 2.002 and 1.939, respectively. Noteworthy, when EDTA was excessive, the grade of Pb was 60.80%, the recovery of galena was 60.86% and the SI value was 2.113. X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Atomic force microscopy, Density functional theory calculation and E-DLVO calculation verified the micro-flotation results and revealed the mechanism of action.
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Arabian Journal of Chemistry
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2024
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IF 5.3
Qinhai Xu,Kang Li,Peng Wang
The reaction mechanism of p -hydroxystyryl-substituted BODIPY (BOH) with two oxidative cations, namely ABTS •+ and Fe 3+ , was investigated in a water–ethanol mixed solution and in liposome suspensions, respectively, using different spectroscopic methods. In solution, the oxidation of BOH (with orange fluorescence) by the two cations occurred at the ethylene group (C C) locating between the dipyrrole and phenol groups and resulted in conjugation-truncated products exhibiting characteristic green fluorescence emission. In heterogeneous small unilamellar vesicles (SUV), water soluble ABTS •+ was evidenced to oxidize BOH embedded in the lipid bilayers of SUV, while Fe 3+ did not. The lack of reaction between Fe 3+ and BOH was attributed to the complexation between Fe 3+ and the phenolic hydroxyl group of BOH on the surface of the SUV. The reaction kinetics results indicated that, in homogeneous solution, the oxidation rate of Fe 3+ was three orders of magnitude slower than that of ABTS •+ for BOH. The location and orientation of BOH within the SUV were discussed based on the reaction phenomena. BOH could be as a good antioxidant fluorescent prober for ABTS •+ detection with a detection limit of 1.5 * 10 −7 M and a linear rang of 0–4.93 μM. What’s more, the amphiphilic BOH dispersed in the round GUV (BOH + GUV) could show the bright red fluorescence. This research suggests the significant potential of BOH as an antioxidant fluorescent probe for in situ bioimaging.
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Microbiology Spectrum
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2024
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IF 3.7
Yanjun Xie,Fangyi Xu,Huali Dong,Jianfeng Mao,Chuanling Zhang
The colonization of Enterococcus in the female vagina leads to neonatal and pediatric enterococcal septicemia. Linezolid (LZD) is a kind of mainstream drug for treating multidrug-resistant Gram-positive infections. OptrA is the main LZD-resistance gene at Enterococci in human isolates. It is essential to explore the prevalence of optrA-carrying Enterococcus in vaginal secretions of late pregnant women and the drug resistance of optrA. From May to June 2023, this study recruited 340 volunteers in late pregnancy (35–40 weeks of pregnancy) to provide non-repetitive vaginal discharge samples. Luria-Bertani broth and florfenicol (10 µg/mL) were used to enrich bacteria. Enterococci was identified through time-of-flight mass spectrometry. Additionally, antimicrobial susceptibility, polymerase chain reaction, and next-generation sequencing assays were applied to this study. Fifty-four optrA-carrying Enterococcus strains were obtained, the proportion of the whole vagina of late pregnant women was 15.88% (54 out of 340), and Enterococcus faecalis account the highest proportion. All optrA-carrying Enterococcus were resistant to at least three drugs. Tetracycline, chloramphenicol, erythromycin, and LZD have higher bacterial resistance rates. Genetic environment analysis revealed that IS1216E, fexA, and erm(A) may synergistically exert multidrug resistance with optrA. It is necessary to strengthen the surveillance of optrA-carrying Enterococcus in pregnant women. This study provides scientific support for controlling hospital infections and managing antibiotic-resistant bacteria, and provides a scientific basis for rational clinical medication.
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PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2024
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IF 6.4
Huaiyu Zhang,Yueli Zhou,Yue Yang,Lunaike Zhao,Hongbo Li,Lijie Long,Junjie Wang,Xuyan Wu,Zhao-Jun Wei
Alternaria alternata , a common fungal pathogen causes black mold in postharvest goji fruit. To explore the in vitro and in vivo impacts of linalool on the fungal infection, we treated A. alternata and infected goji fruit with varying linalool concentrations (0, 0.15, 0.45, and 1.35 mL L −1 ). Linalool repressed hypha growth, spore yield and germination, and germ tube length of A. alternata . Furthermore, we observed abnormal spore and mycelium morphology in the treated samples using optical, scanning electron, and transmission electron microscopy. Linalool reduced lesion diameter and disease incidence in goji fruit. Moreover, transcriptomic analysis of pathogen revealed that linalool inhibited nitrogen metabolism, altered chitin and β -1,3-glucan metabolisms, downregulated the key gene expression related to ergosterol synthesis and sulfur and glutathione metabolisms, regulated the enzymatic antioxidant system, and impacted the proportion of unsaturated fatty acids. Furthermore, linalool damaged the fungal cell wall integrity by inducing alkaline phosphatase and chitinase activities and β -1,3-glucan content and repressing β -1,3-glucanase and chitin synthase activities and chitin levels. Linalool elevated superoxide dismutase and catalase activities and declined peroxidase and glutathione reductase activities and glutathione content in A. alternata , leading to increased H 2 O 2 levels and ROS stress. Our data exhibited the promising antifungal effects of linalool with a future application in management of the postharvest rot of goji fruit.
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MATERIALS RESEARCH BULLETIN
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2024
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IF 5.3
Lili Han,Jihao Zhao,Yifan Miao,Wenqian Zhou,Minglang Yuan,Jinghao Cui,Zhipeng Ci
Copper-based halides have emerged as promising scintillator materials for X-ray imaging, attributed to their favorable photophysical characteristics, such as negligible self-absorption, high light output, and fast decay. Within this category of materials, Cs 3 Cu 2 Cl 5 composite polymer scintillator films stand out, delivering excellent performance as flexible X-ray detector screens. However, severe scattering and light degradation induced by the inevitable agglomeration during recombination restricted the application in high-resolution and flexible X-ray imaging. In this study, a Cs 3 Cu 2 Cl 5 @PMMA film prepared by in-situ growth strategy is successfully developed, which represents uniform area, adjustable flexibility and excellent scintillation performance. The prepared large-area film exhibits green emission at 527 nm, with a high light yield, low detection limit and excellent spatial resolution. Meanwhile, the Cs 3 Cu 2 Cl 5 @PMMA film exhibits a remarkable degree of flexibility, enabling imaging applications on non-planar surfaces. Furthermore, it also demonstrates excellent resistance to high temperature environment, maintaining its high-quality imaging performance at 403 K.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2024
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IF 4.4
Shuang-Shuang Zhang,Jia-Hui Yu,Si-Si Jiang,Lun Wang,Jiong Chen,Jiao Long,Shuang-Xi Gu,Hui Li
P-glycoprotein (P-gp) overexpressed mutidrug resistance (MDR) is currently a key factor limiting the effectiveness of breast cancer chemotherapy. Systemic administration based on P-gp-associated mechanism leads to severe toxic side effects. Here, we designed a T7 peptide-modified mixed liposome (T7-MLP@DTX/SchB) that, by active targeting co-delivering chemotherapeutic agents and P-gp inhibitors, harnessed synergistic effects to improve the treatment of MDR breast cancer. This study established drug-resistant cell models and animal models. Subsequently, comprehensive evaluations involving cell uptake, cell apoptosis, cellular toxicity assays, in vivo tumor-targeting capability, and anti-tumor activity assays were conducted to assess the drug resistance reversal effects of T7-MLP@DTX/SchB. Additionally, a systematic assessment of the biosafety profile of T7-MLP@DTX/SchB was executed, including blood profiles, biochemical markers, and histopathological examination. It was found that this co-delivery strategy successfully exerted the synergistic effects, since there was a significant tumor growth inhibitory effect on multidrug-resistant breast cancer. Targeted modification with T7 peptide enhanced the therapeutic efficacy remarkably, while vastly ameliorating the biocompatibility compared to free drugs. The intriguing results supported the promising potential use of T7-MLP@DTX/SchB in overcoming MDR breast cancer treatment.
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危险品化学品经营许可证（带存储）