Polymers
·
2020
·Vol. 12
·期 12
·3008
·
IF 3.426
Menghui Wan,Haodong Zhao,Lichao Peng,Xueyan Zou,Yanbao Zhao,Lei Sun
In this paper, we propose a facile and cost-effective electrospinning technique to fabricate surface-enhanced Raman scattering (SERS) substrates, which is appropriate for multiple analytes detection. First of all, HAuCl4∙3H2O was added into the TEOS/PVP precursor solution, and flexible SiO2nanofibers incorporated with gold nanoparticles (SiO2@Au) were prepared by electrospinning and calcination. Subsequently, the nanofibrous membranes were immersed in the tannic acid and 3-aminopropyltriethoxysilane solution for surface modification through Michael addition reaction. Finally, the composite nanofibers (Ag@T-A@SiO2@Au) were obtained by the in-situ growth of Ag nanoparticles on the surfaces of nanofibers with tannic acid as a reducing agent. Due to the synergistic enhancement of Au and Ag nanoparticles, the flexible and self-supporting composite nanofibrous membranes have excellent SERS properties. Serving as SERS substrates, they are extremely sensitive to the detection of 4-mercaptophenol and 4-mercaptobenzoic acid, with an enhancement factor of 108. Moreover, they could be utilized to detect analytes such as pesticide thiram at a low concentration of 10−8mol/L, and the substrates retain excellent Raman signals stability during the durability test of 60 days. Furthermore, the as-fabricated substrates, as a versatile SERS platform, could be used to detect bacteria ofStaphylococcus aureuswithout a specific and complicated bacteria-aptamer conjugation procedure, and the detection limit is up to 103colony forming units/mL. Meanwhile, the substrates also show an excellent repeatability of SERS response forS. aureusorganelles. Briefly, the prime novelty of this work is the fabrication of Au/Ag bimetallic synergetic enhancement substrates as SERS platform for versatile detection with high sensitivity and stability.Keywords:Ag/Au nanoparticles;synergetic enhancement;electrospun nanofibers;SERS substrates;versatile detection
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JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Ruiyun You,Jie Li,Haonan Wang,Yaling Wu,Jingzheng Weng,Yudong Lu
Label-free surface-enhanced Raman scattering (SERS) technology is used to screen for colon cancer for its rapid identification, high specificity and non-invasiveness. Here, we have developed a high-performance SERS biosensor that has combined concentration, separation and detection for effective screening of colon cancer. This SERS biosensor is equipped with a centrifuge tube and a filter that consists of two ultrafiltration membranes (UFMs). First, silver nanoparticles (Ag NPs) are uniformly reduced in-situ on the UFM using a controlled “silver mirror reaction”, then the filter and centrifuge tube are assembled into an ultrafiltration centrifuge tube containing Ag NPs (Ag NPs/UFT biosensor). And finally, the Ag NPs/UFT biosensor is applied to concentrate and separate colon cancer serum. The biosensor reduces signal fluctuations in the detection of liquid phase substrates (Ag NPs colloids), which are prone to aggregation and instability, more importantly minimizes the interference of large amounts of small molecule proteins and also concentrates and retains molecular weight proteins of high diagnostic value. Furthermore, we employed SERS spectroscopy by portable Raman, combined with principal component analysis-linear discriminant analysis (PCA-LDA) models, to further assess the screening diagnosis of colon cancer. As a proof of concept, we achieved 100% specificity and 87.5% accuracy with our sensors. This study will contribute to the field of non-invasive, rapid and practical screening for colon cancer together with the ultrafiltration system.
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Nanoscale
·
2019
·Vol. 11
·期 27
·12829-12836
·
IF 6.97
Peng Wu,Lu-Bin Zhong,Qing Liu,Xi Zhou,Yu-Ming Zheng
We have demonstrated a one-step approach for the fabrication of flexible, robust, reproducible and free-standing SERS substrates (AuNPs/polyvinyl chloride (PVC) film) through a polymer induced interfacial self-assembly method. In this method, the polymer (PVC) plays dual roles, that is, inducing the interfacial self-assembly of nanoparticles and fixing the assembled nanostructure in the PVC matrix. As the assembled nanoparticles are orderly half-embedded in the PVC film, the AuNPs/PVC film exhibits outstanding reproducibility and stability. In addition, the film could be easily regenerated by rinsing with NaBH4 solution. As a proof of concept, the film was directly wrapped on an apple surface for in situ detection of pesticide residues, and a detection limit of 10 ng cm−2 thiram was achieved. Furthermore, rapid on-site and in situ detection of multi-pesticide residues has been proved to be feasible with the aid of a portable Raman spectrometer. Due to its simple preparation, good reliability, outstanding stability and reusability, the AuNPs/PVC film has great potential in practical applications.
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ANALYST
·
2018
·Vol. 143
·期 8
·1899-1905
·
IF 3.864
Wei Ji,Xue Zhang,Jianzhang Zhao,Ye Gao,Wei Song,Yukihiro Ozaki
A simple and ultrasensitive surface-enhanced Raman scattering (SERS) assay for heparin detection has been demonstrated based on an in situ hot spot assembly method using N,N′-bis[3,3′-(trimethylammonium)propylamine]-3,4,9,10-perylenediimide (bis-quaternized perylene dye, abbreviated as BQPER). In this assay, a BQPER probe with two positive charges is employed as both Raman reporter and electrostatic bridge to cause the aggregation of citrate-capped Ag nanoparticles (Ag NPs), resulting in a strong SERS signal due to the formation of numerous hot spots in the gaps of the aggregates. The SERS intensity of BQPER decreases in the presence of heparin, because BQPER binds primarily to heparin due to its high anionic charge to mass ratio compared to that of Ag NPs. Accordingly, a novel method for the detection of heparin has been developed based on SERS of the unbound BQPER probe. Two obvious advantages of the proposed heparin assay over other reported methods are: its much wider linear concentration range (10−5–10−10 g mL−1) and lower limit of detection (0.08 ng mL−1). Besides, our proposed heparin assay has been successfully applied to the detection of heparin in serum samples, indicating its great potential in the diagnosis of heparin-related diseases. This work provides a new Raman probe for the SERS analysis of heparin and opens a different perspective for SERS-based transducer design in charged analytes.
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APPLIED SURFACE SCIENCE
·
2024
·
IF 6.3
Xin Liu,Tiehu Li,Yanying Han,Yiting Sun,Amir Zada,Yuhui Liu,Jiahe Chen,Alei Dang
Graphene oxide has attracted wide interest for theoretical research and application exploration in surface enhancement Raman scattering (SERS). However, how to break the geometric limitations of the nanoscale and extend its properties to the macroscopic level remains an urgent problem. Here, we prepared flexible graphene oxide/Au nanoparticles (GO/AuNP) fibers with high sensitivity and reproducibility for SERS sensing using wet spinning method. Benefitting from the plasmonic coupling of GO/AuNP fibers in vertical direction and uniform adsorption of the target molecules, the limit of detection for fiber was as low as 5 × 10 -11 M for rhodamine 6G (R6G) and crystal violet (CV) molecules. In addition, due to the optimal distribution of AuNP in the macroscopic assembly of GO/AuNP hybrid, the fiber substrates provided uniform and stable signals for both temporal (6 months) and spatial (RSD = 8.18 %) scales, exhibited excellent reliability and durability. Moreover, an ultra-low concentrations of the pesticide thiram residues could be rapidly traced by direct sampling from the apple peel with a sensitivity of 5 × 10 -9 M, which is much lower than the residue limit set by the US environmental protection agency. We envision that this work could provide new insights into the large-scale and economical fabrication of high-performance flexible SERS sensors for real world applications.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Qijia Zhang,Xiaodi Ma,Xiaoyu Du,Peng Song,Lixin Xia
We report a surface magnetic solid-phase extraction–surface-enhanced Raman scattering (SMSPE-SERS) method based on silver-nanoparticle-coated Fe 3 O 4 /chitosan (Fe 3 O 4 /CS@Ag) microspheres as the substrate, and this method integrates all steps from sample pretreatment to detection. Fe 3 O 4 /CS was synthesized by a one-step solvothermal method in which chitosan (CS) was used as a surface modifier and adsorbent. Fe 3 O 4 /CS@Ag microspheres exhibit both adsorption ability and SERS activity. Therefore, we used the SMSPE-SERS method to detect pesticide residues on fruit peel. The procedures of capturing, separating and enriching pesticides, as well as detection, are all integrated. In addition, the SERS substrate allows label-free detection of thiram pesticide in both fruit peel and apple juice. Owing to the uniform distribution of Ag NPs and the adsorption ability of CS, the thiram-detection sensitivity was sufficiently high to detect the lowest concentration of 1.2 ng/cm 2 , which was significantly lower than the maximum thiram residue limit (7 μg/cm 2 ) in fruits. The method was comparable to high-performance liquid chromatography with recovery ranging from 86.60 to 109.69 %.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Yansheng Liu,Zhenle Qin,Guofu Wang,Xiaobo Jia,Jin Zhou,Hongqi Li,Zhaoxu Li,Xiaohui Fang
Nanoplastic particles (NPPs) have attracted lots of attention due to their toxicity. In this study, a Surface-enhanced Raman scattering (SERS)-based category on selectivity and quantification detecting the polystyrene (PS) NPPs has been presented. Firstly, the size-dependent SERS relationship between the diameter of Ag nanocavities (AgNCAs) and the diameter of the PS NPPs is studied. By continuously dripping the PS NPPs on proposed AgNCAs substrates, AgNCAs exhibit excellent enrichment capability with a promoted limit of detection (LOD) of 0.001 mg/mL. Secondly, thermally evaporated Ag nanoparticles (AgNPs) as an enhancement layer are used to form the AgNPs/PS NPPs/AgNCAs sandwich structure with a SERS enhancement of 300 %. Thirdly, a SERS microfluidic chip constructed by integrating two kinds of pore size (87 nm and 356 nm) AgNCAs is fabricated to selectivity quantifying absolute concentration of the mixed PS NPPs with different diameters in a mixed solution. It shows excellent performance. This novel category proves a good method for identifying plastic nanoparticles and analyzing their size distribution existing in the surroundings indicating good practical applications.
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Food Chemistry-X
·
2024
·
IF 6.5
Yun Wu,Meiqi Wang,Ying Gu,Kun Ge
Herein, the miniaturized thermal purge-and-trap (MTPT) device combined with self-calibration colorimetric/surface-enhanced Raman spectroscopy (SERS) dual-model optical sensors were designed for effective analysis of sulfur dioxide (SO 2 ) in wine. The SO 2 can be rapidly separated from wine and enriched by MTPT device, ensuring colorimetric/SERS dual-model optical sensing based on Karl Fischer reaction. The high separation efficiency of miniaturized MTPT device combined with self-calibration of dual-model optical sensors significantly alleviate matrix interference and improve the detection accuracy. The satisfactory linear range of 0.1–200.0 mg/L and 0.1–500.0 mg/L with limit of detection of 0.03 mg/L can be obtained. Finally, the MTPT-colorimetric/SERS method was applied to determine the content of SO 2 in different kinds of wine to verify the practicality. These results provide an ideal strategy in construction of MTPT device combined with self-calibration dual-model optical sensors for quantification of gaseous hazards in complex food samples with high rapidity, anti-interference and accuracy.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2018
·
IF 3.141
Wen-Jing Chen,Xiao-Qing Liu,Shun Zhang,Hong Jiang
The synthesis of new surface-enhanced Raman scattering (SERS) substrates for the detection of trace poisonous organic pollutants is important for environmental monitoring. Herein, we designed and prepared monodispersed and uniform Ag NPs anchored on silica microspheres (Ag@ASMs) by the orientation of grafted amino groups and applied it as a SERS substrate for the detection of ultralow concentrations of poisonous organic pollutants (Rhodamine 6G (R6G), crystal violet (CV), and melamine) with high sensitivity and reproducibility in aqueous solution. A ratio of 2:1 Ag@ASMs exhibited optimum SERS performance, allowing the detection of 10–12 M of R6G with an enhancement factor of 6.36 × 107 (collect the normal Raman signal based on 10–4 M R6G) as well as 10–8 M of CV and melamine. The density functional theory calculation also provided evidence that modified 3-triethoxysilylpropylamine molecules are interspaced, and the optimum Ag dosage further tuned the gap between Ag NPs as well as the size of Ag NPs. This approach can suggest new ideas for the design and synthesis of other improved SERS-active substrates.
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Journal of Materials Chemistry C
·
2019
·Vol. 8
·期 6
·2142-2154
·
IF 6.641
Zhiyuan Yang,Hongmei Liu,Yiran Tian,Ying Chen,Zhiqiang Niu,Canliang Zhou,Fuyan Wang,Chenjie Gu,Shiwei Tang,Tao Jiang,Jun Zhou
In the field of surface-enhanced Raman scattering (SERS)-based immunoassays utilizing a sandwich structure, one of the main barriers to overcome is the development of an immunoprobe and substrate with ultrahigh sensitivity and relatively good reproducibility. In this work, a novel gold (Au)-coated “stellate” mesoporous SiO2 (SMSiO2) hybrid structure was proposed and applied as an immunoprobe. It was noticed that the SMSiO2 matrix with an appropriate coating amount of Au nanoparticles (NPs) demonstrated a much higher SERS intensity than solid SiO2 (SSiO2)@Au NPs with an enhancement ratio of 2.6. Electromagnetic “hot spots” existed around the sharp edges and ultrasmall nanogaps of the multibranched SiO2 matrix were verified to be the main reason responsible for the conspicuous enhancement given by the simulation results. Meanwhile, a hydrophilic–hydrophobic SERS-active immunosubstrate was prepared by linking antibodies onto the hydrophilic Ag-deposited sandpaper assembled with hydrophobic-treated filter paper. Such a novel substrate maintained excellent SERS homogeneity with quite a low relative standard deviation (RSD) of 12.85% arising from the elimination of the coffee-ring effect. The constructed sandwich immunoassay structure using the developed immunoprobe and the substrate was successfully employed in a specific assay for the ferritin (FER) antigen and exhibited an extensive dynamic linear range from 1 × 10−5 to 3 × 10−13 g mL−1 with an ultralow limit of detection (LOD) of 3.16 × 10−14 g mL−1. The simplicity and versatility of this strategy demonstrated its superior sensitivity for tumor markers and it can be expected to have potential practical application in the clinical diagnoses of cancer.
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Biomedical Optics Express
·
2025
·Vol. 16
·期 5
·2033-2046
·
IF 3.2
Binbin Zeng,Huaiwu Fu,Cong Wu,Xudong Zhang,Tianran Li,Qiong Xu,Yayun Qian,Yayun Qian,Ruirui Yang,Ruirui Yang
In this study, we constructed a SERS assay platform that can be used for the ultrasensitive detection of cerebral infarction (CI)-associated miR-106a-5p based on the recognition competition strategy and the highly efficient catalytic activity of Au@Pt NRs nanoenzymes. When the target miR-106a-5p is present in the detection solution, it binds to the nucleic acid aptamer (cDNA), leading to the detachment of its complementary strand (H1) carrying Au@Pt NRs from the surface of AuNHs, and the oxidation of TMB is reduced, resulting in the decrease of SERS signal. In addition, this method enables single-step, ultrasensitive detection of target miRNAs in patient serum with a limit of detection (LOD) of 0.96 fM, which is consistent with the results of real-time quantitative polymerase chain reaction (qRT-PCR) and fluorescence. This method provides a novel approach for the effective detection of miRNAs, which is of great clinical value for the early detection of CI.
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TALANTA
·
2022
·
IF 6.556
Ming Guan,Xiaojing Kang,Le Wei,Xingjun Hu,Chao Han,Xing Li,Hanwen Liu,Liang Qu,Zhenwen Zhao
Silk, as one of the representative artifacts of China, profoundly affects the communication between eastern and western civilizations, and dyes, as the color support of silks, reflected crucial historical, cultural and technological information. Surface enhanced Raman spectroscopy (SERS) characterized by vibrational information has been extensively employed in dye analysis. However, since natural plants with complex coloring compositions in ancient China were broadly applied in dying textiles, the existing SERS methods often misinterpret results in dye analysis. Besides, semi-quantification of each component was of great difficulty by SERS, limiting the exquisite comparative analyses of different historical samples. For the first time, a dual-mode strategy combining SERS with high mass resolution MALDI FTICR MS was developed in virtue of core-shell silver nanoparticles (AgNPs@PDA), which realized the precise identification and semi-quantification of complex dye mixtures, thus significantly improving the accuracy and applicability of traditional SERS method. Four typical dye components (alizarin, purpurin, berberine and indigo) have been identified and semi-quantified in unearthed dyed silks from Tang Dynasty based on the method. More interestingly, multiple dye components with different contents and their ratio could be precisely determined, which might help in further investigating their dyeing techniques. This dual-mode strategy represents a promising tool for providing solid support for cognition, evaluation and restoration of textile objects in museums and conservation centers.
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