Food and Bioprocess Technology
·
2019
·Vol. 12
·期 9
·1537-1547
·
IF 3.032
Yang Zhikun,Zou Xiaobo,Li Zhihua,Huang Xiaowei,Zhai Xiaodong,Zhang Wen,Shi Jiyong,Tahir Haroon Elrasheid
The present work aimed at evaluating the efficacy of gum Arabic–based edible coating enriched with red and white roselle extracts, on the postharvest quality of blueberry fruits during storage at 4 ± 0.5 °C. Physicochemical, phytochemicals, antioxidant capability, decay percent, and microbiological analyses of coated and uncoated blueberries were performed. The change rates of polyphenol oxidase (PPO) and guaiacol peroxidase (POD) activity were also measured. The coatings (gum Arabic with or without roselle extract) inhibited the growth of microbes (yeast and mesophilic aerobic bacteria) and enzyme activities (POD and PPO) of blueberries, reduced the degradation of anthocyanins and total phenolic as well as the weight loss and decay, and improved the firmness of blueberries. In comparison with gum Arabic coating, the gum Arabic enriched with roselle extract coating showed better performance on reducing microbes, enzyme activities, and anthocyanins degradation while enhancing the total phenolic content and decreasing the decay percentage. No significant differences were observed between the coated and uncoated blueberries in terms of their antioxidant capacities and pH values ( p > 0.05). In conclusion, the combination of gum Arabic and roselle extract as edible coating materials can be used to conserve the quality of blueberry.
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高影响因子
IONICS
·
2019
·Vol. 25
·期 12
·5809-5818
·
IF 2.289
He Yuanhong,Lin Yangfan,Jiang Jingwei,Yang Deren,Du Ning,He Xueqin,Ren Jianguo,He Peng,Pang Chunlei,Xiao Chengmao,Chen Yifan,Bao Liang
Silicon is regarded as the next-generation alternative anode material of lithium–ion battery due to the highest theoretical specific capacity of 4200 mAh g −1 . Nevertheless, the drastic volume expansion/shrink (~ 300%) during the lithiation/delithiation process and the poor electrical conductivity obstruct its commercial application. Herein, we report a novel design of litchi-structural Si@C nanoparticles (L-S Si@C) as long cycle life anode material for lithium–ion battery. The L-S Si@C with nanosized (~ 50 nm) SiC dispersed in the inner surface and ~ 6 wt% carbon layer covering the outer surface delivers a specific capacity of 1145 mAh g −1 in the initial cycle and maintains a specific capacity of 570 mAh g −1 after 300th cycling times, which can be attributed to the specially structurally stable L-S Si@C nanoparticles.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
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2019
·Vol. 92
·期 1
·134-145
·
IF 1.986
Xia Lijian,Yao Binbin,Shi Hao,Shi Zhekun,Wang Wenpin,Kan Ze
In this paper, we report the fundamental method for controlling monodispersed silica nanoparticles size ranged from 10 to ~135 nm assisted by lysine or lysine hydrochloride, during which the seed regrowth technique was utilized. The synthesis was prepared in the water-base system, which was environment friendly and convenient. According to our research, the influence factors of co-solvent system, molar content of additional TEOS, and molar ratio composition of additional TEOS/seeds were confirmed. The final morphology and size of silica nanoparticles can be tuned by changing the amount of additional TEOS and the seeds size. To guarantee the monodispersity of final particles. In the regrowth process, cyclohexane used as the co-solvent floated on the surface of water to block additional TEOS contact with water directly, and made the silicate intermedium consumable for the growth of the parent particles immediately, while not forming new nucleation. In such a condition, to lower the cost and prepare mass production, the lysine was replaced by lysine hydrochloride, meanwhile, triethylamine was introduced to neutralize the hydrochloride. It should be noted that as the particle size increased to be more than 100 nm, when usinglysine hydrochloride and triethylamine, the types of nitrogen adsorption–desorption isotherms changed from H1 to H3; in contrast, the isotherms of the particles were changed from H2 to H1 when catalyzed by lysine.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Yang Meiling,Liu Mingwang,Wu Zipeng,He Yu,Ge Yili,Song Gongwu,Zhou Jiangang
Nitrogen and chlorine dually-doped carbon dots (N,Cl-CDs) were hydrothermally prepared starting from 4-chloro-1,2-diaminobenzene and dopamine. The N,Cl-CDs exhibit strong orange fluorescence, with excitation/emission maxima at 420/570 nm and a relative high quantum yield (15%). The N,Cl-CDs were employed to detect acetylcholinesterase (AChE) activity and organophosphate pesticides (OPs) which are enzyme inhibitors. Acetylthiocholine is enzymatically split by AChE to produce thiocholine which triggers the decomposition of Ellmans’s reagent to form a yellow colored product (2-nitro-5-thiobenzoate anion). The product causes an inner filter effect (IEF) on the fluorescence of the N,Cl-CDs. Fluorescence decreases linearly in the 0.017 to 5.0 Unit·L −1 AChE activity range, and the detection limit is 2 mUnit·L −1 . If organophosphates are present, the activity of AChE becomes increasingly blocked, and this leads to a less expressed IFE and an increasing recovery of fluorescence. This was used for the quantification of OPs. Response is linear in the 0.3–1000 μg·L −1 OP concentration range with a 30 ng·L −1 detection limit.
Schematic representation of the synthesis of nitrogen and chlorine dually-doped carbon dots (N,Cl-CDs) and the recognition of organophosphate pesticides by N,Cl-CDs.
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高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2019
·Vol. 139
·期 3
·2079-2087
·
IF 2.471
Ou Juhua,Ge Chao,Kong Zhenxing,Chen Yujing,Dai Zilin
A novel organo-modified α-zirconium phosphate was prepared by intercalating a functional 1,2,2,6,6-pentamethyl-4-(phenyldiethoxysiloxy) piperidine into α-ZrP. The structure of functional α-ZrP (IZrP) was characterized by X-ray diffraction, FTIR and SEM. The thermal stability of the silicon rubber (SR) composite obtained by physically mixing IZrP was investigated by TGA and DSC. The results shown that the maximum decomposition temperature ( T max ) of SR/IZrP composites increased from 514 to 540 °C as the IZrP loading increased from 0 to 5 mass% and a reduction in exothermic enthalpy from 3378 to 1520 J g −1 was observed. The maximum exothermic peak temperature had obvious enhancement from 557 to 593 °C, while in SR/IZrP/AlOH the deteriorated thermal stability was observed at T 0.1 when compared with SR/IZrP. The addition of AlOH into SR/IZrP increased the exothermic enthalpy, prolonging the exothermic process and increasing the maximum exothermic peak temperature. The SEM results revealed that IZrP and AlOH were dispersed in SR with the layered structure.
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高影响因子
JOURNAL OF CLUSTER SCIENCE
·
2019
·Vol. 31
·期 2
·337-346
·
IF 2.125
Zhao Chenyu,Jiao Junfeng,Zhou Wencui,Zhang Yong,Liu Han,Yang Xiaogang,Pandi Boomi,Cai Yurong
In this study, a new HAP/rGO/AuNPs-AO modified carbon electrode (GCE) was fabricated for amperometric detection of ascorbic acid. The HAP/rGO/AuNPs composite was prepared by one-step hydrothermal method. The large specific surface area, strong adsorption and excellent biocompatibility of HAP nanowires enabled fast, stable and accurate detection of ascorbic acid. As array of quantum dot, AuNPs had strong electrocatalytic activity in addition to good adsorption to enzymes, which could provide high sensitivity and low limit of detection for biosensor. Then rGO ensured that current signal could be quickly transferred from the composite to electrode. Under the optimum conditions, the prepared biosensor demonstrated a wide linear range from 0.39 to 36 mM (R 2 = 0.99845), a low limit of detection (3.39 μM, S/N = 3) and a high sensitivity (15.949 mA moL −1 ) with good selectivity, stability (10.8%) and repeatability (3.9%), which might providing a reasonable and reliable way for ascorbic acid detection.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-10
·
IF 5.479
Cong Lili,Liang Lijia,Cao Fanghao,Sun Dan,Yue Jing,Xu Weiqing,Liang Chongyang,Xu Shuping
A high-throughput, dual-channel single cell analytical method is described for the detection of sialic acid (SA) on single cell based on the use of microfluidic droplets integrated with plasmonic imaging and surface-enhanced Raman spectroscopy (SERS) with the assistance of a multifunctional metal nanoparticle-based probe. The multifunctional plasmonic nanoprobe was prepared by modifying silver nanoparticles (AgNPs) with 4-mercaptophenylboronic acid (MPBA) that both warrants SA recognition and acts as a Raman reporter. This nanoprobe is a high-contrast indicator under bright field imaging due to the strong energy loss feature of AgNPs, and also owns possesses a strong SERS enhancement capability toward MPBA. Cells incubated with the plasmonic nanoprobes were isolated in water-in-oil droplets and then were re-dispersed in a chamber array chip. High-precision profiles of SA on a single cell in one droplet were obtained by the bright field imaging and image processing. The SA expression levels on different cell lines (MCF-7, HepG2, SGC and BNL.CL2) traced by SERS spectroscopy were compared. The statistical data among different cell lines confirm that the SA expression levels on cancer cells are much higher than that on normal cells. Single cell analysis further revealed that the cell-to-cell variations are more obvious in cancer cell lines. This study provides a valuable tool for understanding glycan-related biochemical processes.
A high-throughput, dual-channel microfluidic droplet platform succeeded in distinguishing different cancer cell lines at single living cell level integrated with plasmonic imaging and surface-enhanced Raman spectroscopy with assistance of a multifunctional metal nanoparticle-based probe.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 54
·期 11
·8216-8225
·
IF 3.442
Xu Wen,Cao Bo,Lin Hechun,Cheng Wenjuan,Luo Chunhua,Peng Hui,Duan Chungang
The catalytic activities of three topotactic phase strontium cobaltites (SCO, hexagonal, orthorhombic and tetragonal) for H 2 O 2 decomposition were studied. All they present excellent catalytic activity with good stability at cycled test, and the mesoporous tetragonal SCO exhibits the best activity. Detailed kinetic research indicates the zero-order process of H 2 O 2 decomposition. Increasing the temperature will accelerate the reactions greatly with the controlled temperature in the range of 20–45 °C. The calculated active energy ( E a ) for H-SCO, O-SCO and T-SCO is 93.2, 85.1 and 87.5 (kJ/mol), respectively. The efficient catalytic degradation of Rhodamine B illustrates that SCO is a kind of promising catalysis for the advanced oxidation process.
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高影响因子
Microbial Cell Factories
·
2019
·Vol. 18
·期 1
·1-10
·
IF 4.402
Xu Jian-Miao,Li Jian-Qiang,Zhang Bo,Liu Zhi-Qiang,Zheng Yu-Guo
Background l -2-aminobutyric acid ( l -ABA) is an unnatural amino acid that is a key intermediate for the synthesis of several important pharmaceuticals. To make the biosynthesis of l -ABA environmental friendly and more suitable for the industrial-scale production. We expand the nature metabolic network of Escherichia coli using metabolic engineering approach for the production of l -ABA. Results In this study, Escherichia coli THR strain with a modified pathway for threonine-hyperproduction was engineered via deletion of the rhtA gene from the chromosome. To redirect carbon flux from 2-ketobutyrate (2-KB) to l -ABA, the ilvIH gene was deleted to block the l -isoleucine pathway. Furthermore, the ilvA gene from Escherichia coli W3110 and the leuDH gene from Thermoactinomyces intermedius were amplified and co-overexpressed. The promoter was altered to regulate the expression strength of ilvA* and leuDH . The final engineered strain E. coli THR Δ rhtA Δ ilvIH /Gap- ilvA *-Pbs- leuDH was able to produce 9.33 g/L of l -ABA with a yield of 0.19 g/L/h by fed-batch fermentation in a 5 L bioreactor. Conclusions This novel metabolically tailored strain offers a promising approach to fulfill industrial requirements for production of l -ABA.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 54
·期 11
·8332-8345
·
IF 3.442
Wang Qianqian,Cui Jiuyun,Liu Siwei,Gao Jia,Lang Jihui,Li Chunxiang,Yan Yongsheng
The worldwide oily wastewater is one of the most serious environmental problems. The current membrane separation technology has received an increasing attention toward oil/water emulsions separation. Here, a high-efficient superhydrophilic and underwater superoleophobic membrane with improved antifouling property was fabricated based on modified halloysite nanotubes anchored at polyvinylidene fluoride/graphene oxide membrane surface via polydopamine adhesive. The composite membrane exhibited an outstanding antifouling performance as well as high pure water flux (1500 L m −2 h −1 ) under gravity driven. Besides, the as-prepared composite membrane could separate both surfactant-free and surfactant-stabilized oil/water emulsions with separation efficiency up to 99.5%. Moreover, this facile preparation and eco-friendly composite membrane provides promising practical application toward environmental remediation.
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Wang Ying,Hou Yajun,Li Hanxia,Yang Mingfeng,Zhao Peng,Sun Baoliang
A surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) is described for the quantitative analysis of the proteinic stroke biomarker S100-β that has to be detected at very low concentration levels. The Raman reporter 5,5′-dithiobis-2-nitrobenzoic acid (DTNB) on gold nanoparticles (GNPs) was employed as the SERS tags. They are shown to perform much better than bare GNPs in LF strips. The S100-β protein can be detected by this method with very low detection limits by monitoring the intensity of the characteristic Raman peak of the S100-β protein-conjugated GNPs at 1332 cm −1 . Under optimized conditions, the assay works in the 1 pg·mL −1 to 40 ng·mL −1 S100-β concentration range, and the detection limit is as low as 0.14 pg·mL −1 . This is lower by a factor of 3 compared to colorimetric or fluorimetric methods.
Schematic illustration of the configuration (A) and the principle of the SERS-based lateral flow assay for quantification of S100-β (B).
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高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 7
·1-9
·
IF 5.479
Yu Qiongqiong,Liu Jing,Zhao Guangying,Dou Wenchao
An immunochromatographic assay (ICA) is presented that can be applied to simultaneous detection of clenbuterol (CLE) and ractopamine (RAC). It is making use of two red and blue silica nanoparticles (SiNPs) that act as labels for encoding the antibodies. This design permits multiplexed analysis in a single test line and does not require an external source for photoexcitation. Anti-CLE was labeled with red SiNPs, and anti-RAC with blue SiNPs. The capture antigens CLE-BSA and RAC-BSA were placed onto the conjugate pad and the test line of the test strip, respectively. Under bare eye examination, no cross-colored lines or nonspecific bioconjugate adsorption were observed, and the visible limit of detections for CLE (red) and RAC (blue) are 3 and 2 ng‧mL −1 , respectively. This design allows for multiplexed detection with reduced device dimensions and costs, and with easy integration and manufacturing. Conceivably, the method may be extended to simultaneous determination of numerous other analytes.
The principle of qualitative detection strategy of multiplex immunochromatographic assay for clenbuterol (CLE) and ractopamine (RAC) is schematically illustrated. Depending on the type and ratio of organic dyes, the color of colored silica nanoparticle can be tuned from red to purple and even to black (lower right corner).
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高影响因子
危险品化学品经营许可证（带存储）