MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-13
·
IF 5.3
Li Guiyin,Yan Ruijie,Chen Wei,Wu Runqiang,Liang Jintao,Chen Jiejing,Zhou Zhide
Golgi protein 73 (GP73) is a new serum marker associated with early diagnosis and postoperative assessment of hepatocellular carcinoma (HCC). Herein, an electrochemical/fluorescence dual-signal biosensor was designed for determination of GP73 based on molybdenum disulfide/ferrocene/palladium nanoparticles (MoS 2 -Fc-PdNPs) and nitrogen-doped graphene quantum dots (NGQDs). GP73 aptamer (Apt) was labeled with NGQDs to form the NGQDs-Apt fluorescence probe. MoS 2 -Fc-PdNPs served not only as the fluorescence quencher but also as electrochemical enhancer. The sensing platform (NGQDs-Apt/MoS 2 -Fc-PdNPs) was formed based on the fluorescence resonance energy transfer (FRET) mechanism. In the presence of GP73, the specific binding of NGQDs-Apt to GP73 interrupted FRET, restoring the fluorescence of NGQDs-Apt at λ ex/em = 348/438 nm and enhancing the oxidation current of Fc in MoS 2 -Fc-PdNPs at 0.04 V through differential pulse voltammetry (DPV). Under the optimal conditions, the DPV current change and fluorescence recovery have a good linear relationship with GP73 concentration from 1.00 to 10.0 ng/mL. The calibration equation for the fluorescence mode was Y 1 = (0.0213 ± 0.00127) X + (0.0641 ± 0.00448) and LOD was 0.812 ng/mL (S/N = 3). The calibration equation of the electrochemical mode was Y 2 = (3.41 ± 0.111) X + (1.62 ± 0.731), and LOD of 0.0425 ng/mL (S/N = 3). The RSDs of fluorescence mode and electrochemical mode after serum detection were 1.62 to 5.21% and 0.180 to 6.62%, respectively. By combining the electrochemical and fluorescence assay, more comprehensive and valuable information for GP73 was provided. Such dual-mode detection platform shows excellent reproducibility, stability, and selectivity and has great application potential. Graphical Abstract
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高影响因子
JOURNAL OF POROUS MATERIALS
·
2024
·Vol. 31
·期 3
·793-808
·
IF 2.5
Yang Zhaosheng,Tang Guoqiang,Li Yichuan,Niu Xiaoyue,Cheng Lingyao,Chai Yongming
TS-1 molecular sieve spherical membranes loaded on small-sized carriers with hierarchical pore structures and high framework-titanium contents could both exhibit high catalytic performances and facilitate the separation from the products after the reaction. In this paper, a simple in situ static crystallization method was used to achieve the construction of hierarchical porous TS-1 molecular sieve membranes on the surfaces of 400-µm spherical ZrO 2 carriers using cetyltrimethylammonium bromide (CTAB) and soluble starch as mesoporous templating agents. Through the catalyst characterization and performance evaluation, soluble starch was finally selected as the mesoporous templating agent, and the film-forming mechanism was investigated by examining the effects of the proportion of precursor liquid and the crystallization conditions. In the chloropropene epoxidation reaction, the effective utilization of H 2 O 2 and the selectivity of epichlorohydrin (ECH) reached 96.72% and 93.85%, respectively.
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FIBERS AND POLYMERS
·
2024
·Vol. 25
·期 3
·797-805
·
IF 2.2
Zhang Xi,Shi Zhengjun,Xu Pengfei,Zhang Qi,Huang Xudong,Xing Guiying,Shao Linjun,Qi Chenze
Supported cobalt catalysts have attracted more and more attention for their easy separation and recyclability. Herein, cobalt-embedded pyridine functionalized polystyrene superfine fibers were readily fabricated by electrospinning. Then, the pyridine functionalized polystyrene and polystyrene molecules inside the fibers were cross-linked by paraldehyde to improve the fiber stability. The synthesis of pyridine functionalized polystyrene and cross-linking of polystyrene molecules inside the fibers were confirmed by FT-IR spectra. The fiber morphologies were characterized by SEM. The dispersion of cobalt species was analyzed by SEM–EDS, XRD and TEM. Finally, the Heck reactions were employed to evaluate the catalytic performance of this novel fibrous catalyst. The catalysis results show that this novel fiber catalyst exhibited excellent catalytic activity for the Heck reactions of aromatic iodides with alkenes to afford coupling products with yields over 80%. Moreover, due to the embedment of cobalt species and chelation of pyridine groups, this novel fiber catalyst can be reused five times with little loss of catalytic activity. In all, we have developed a highly active and stable supported cobalt catalyst, which holds great potential for green chemical processes.
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CELLULOSE
·
2023
·Vol. 30
·期 14
·8997-9012
·
IF 5.7
Peng Qiao,Chen Yi,Fan Guozhi,Song Guangsen,Cheng Qunpeng
Holocellulose extracted from wheat straw was acetylated and used to prepare biodegradable composite film. An efficient catalytic system for the acetylation of holocellulose was explored by evaluating the influence of type and the amount of catalyst on the degree of substitution (DS) of acetylated holocellulose (AHC). The DS of AHC reached 2.16 when using p -toluene sulfonic acid and sulfuric acid as co-catalyst. AHC was further used to prepare composite film in the presence of reinforcer and plasticizer, and the influence of DS value of AHC on the properties of composite film was investigated. The tensile strength, elongation at break and Young’s modulus of the composite film derived from AHC with DS value of 2.16 were 9.51 MPa, 82.66% and 54.43 MPa when using chitosan and polyvinyl alcohol as the reinforcers, sorbitol and glycerol as the plasticizers. The UV-blocking performance and degradation of AHC-based composite film were also performed. It was found that about 90% UV light was absorbed and 59.2% was decomposed after biodisintegrating for 30 d. The excellent comprehensive performance of the AHC-based composite film reveals application prospect of holocellulose in the field of biodegradable materials.
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高影响因子
JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Pang Shukui,Yu Yanchao,Yan Xuexue,Wu Mianyuan,Liu Qiye,Zu Panru,Wu Canyao
A novel coumarinylhydrazone fluorescent probe L was designed and synthesized from 4-(diethylamino)salicylaldehyde, its structure was characterized by NMR, IR. Fluorescence emission spectra showed that in ethanol solution, probe L could form a 1:1 complex L-Cu 2+ with Cu 2+ to realize the "turn-off" detection of Cu 2+ with high specificity and sensitivity (3.7 × 10 –7 mol/L). Meanwhile, the complex L-Cu 2+ had a specific fluorescence-enhanced response to HPO 4 2− with a detection limits down to 5.6 × 10 –7 mol/L and was resistant to the effects of many common anions (NO 2 − , N 3 − , CO 3 2− , SO 3 2− , HPO 4 2− , I − , Br − , F − , HCO 3 − , SO 4 2− , NO 3 − , Cl − , CH 3 COO − , Cr 2 O 7 2− , S 2 O 3 2− , P 2 O 7 4− ). Detection mechanism could be HPO 4 2− captured Cu 2+ of the complex and released the free ligand L. At last, the complex L-Cu 2+ was successfully applied to the determination of HPO 4 2− in different environmental water samples, and the spiked recoveries ranged from 98.05% to 108.18% and the relative standard deviations of 0.75% ~ 2.9%, which had good application prospects. Graphical A new coumarin acyl hydrazone fluorescent probe L was designed and synthesized, which could specifically recognize Cu2+ and HPO42- in a relay manner. In addition, the probe had good prospects for practical applications.
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高影响因子
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
·
2024
·Vol. 397
·期 8
·6037-6050
·
IF 3.1
She Cheng-Ye,Deng Yuan-Xiong,Wu Qin-Yu,Li Jing
Crocetin as one of the main components of saffron possesses a lot of pharmacological effects, especially the beneficial effects in the treatment of hyperlipidemia. However, the pharmacokinetics of crocetin in the pathological state of hyperlipidemia has not been reported. In present study, the pharmacokinetics of crocetin in hyperlipidemia rats after oral administration of crocetin was investigated and the possible mechanisms for the pharmacokinetics were explored. High-fat diet was used to induce hyperlipidemia in rats. The pharmacokinetics of crocetin was investigated in hyperlipidemia and normal rats after oral and intravenous administration of crocetin, and the possible mechanisms of the pharmacokinetic changes were investigated in terms of metabolism and absorption using in vitro incubation with liver microsomes and the everted gut sac method, respectively. Results indicated that the AUCs of crocetin in hyperlipidemia rats after oral administration of crocetin were remarkably decreased when compared with those in normal rats. Moreover, crocetin was also metabolized more rapidly in the liver microsomes of hyperlipidemia rats and intestinal absorption of crocetin was significantly reduced in hyperlipidemia rats. It suggested that the remarkably decreased AUCs of crocetin in hyperlipidemia rats might partly result from the result of faster metabolic elimination and reduced absorption of crocetin in the hyperlipidemia pathological state. And the present investigations conducted on rats demonstrate that further investigations into the kinetics of crocetin in humans with hyperlipidemia are necessary in order to ensure an adequate dosage in this indication.
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高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
·
2023
·Vol. 39
·期 11
·1-17
·
IF 4.1
Song Xiaohui,Li Tang,Gu Hui,Yin Heng
Aspergillus is a well-studied fungal genus that is widely used in the processing of plant biomass in industries. This study investigated the effects of space exposure on the ability of Aspergillus costaricaensis , a filamentous fungus isolated from rotten orange peel, to degrade pectin. These fungal spores were carried into space by the Long March 5B carrier rocket and exposed to cosmic radiation for 79 h. After the flight, these spores were resuscitated, and then the growing strains were screened with pectin as the sole carbon source, and the pectinase activity was evaluated. A mutant with increased biomass accumulation ability and pectin-degrading activity compared to the ground control strain was obtained. Comparative transcriptome analysis revealed that several CAZymes genes were significantly upregulated in the mutant, especially those related to pectin degradation. Among the 44 pectinases identified from the annotated genome, 42 were up-regulated. The activities of these pectinases are able to synergistically break down the structure of pectin. In addition, the expression of some genes involved in metabolism, sugar transport, and stress response was altered. These results imply that space exposure might serve as a potential mutagenesis breeding technique, offering the opportunity to acquire biomass-degrading microbial strains with potential for industrial application.
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JOURNAL OF FLUORESCENCE
·
2022
·Vol. 33
·期 3
·965-972
·
IF 2.525
Zheng Yabin,Li Chen,Li Quan,Zhang Taoyi,Chen Jing,Ji Wenxi,Wei Yun
Sodium tripolyphosphate, a food additive, is applied broadly in food industry. However, excessive accumulation of sodium tripolyphosphate can result in electrolyte abnormality of the body. Therefore, it is of great importance to investigate an effective method for the detection of sodium tripolyphosphate. In this work, nitrogen-doped carbon dots (NCDs) with constant fluorescence were fabricated using a domestic microwave oven. A ratiometric fluorescent probe was constructed in which NCDs were as internal standard, calcein & Eu 3+ were as the detection signal. The fluorescence of calcein at 515 nm was quenched by Eu 3+ , whereas the emission peak of NCDs at 446 nm was almost unchanged. Additionally, the fluorescence of calcein was recovered because of the strong interaction of sodium tripolyphosphate and Eu 3+ . The linear range for sodium tripolyphosphate was 0.5-6 µmol/L with detection limit of 0.12 µmol/L. Furthermore, the ratiometric fluorescent probe was applied for sodium tripolyphosphate detection in real milk samples.
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高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2016
·Vol. 21
·期 3
·715-724
·
IF 2.327
Zhang Kaiyue,Chen Si,Feng Yaqing,Shan Zhongqiang,Meng Shuxian
A novel polymer gel electrolyte was used to improve the performance and long-term stability in dye-sensitized solar cells (DSSCs). The polymer gel electrolyte (PGE) was prepared by mixing 5 wt% poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) and 2 % TiO 2 nanoparticles. The conductivity of PGE with P25 reached 9.98 × 10 −3 S/cm, which increased by 34.9 % compared with 7.40 × 10 −3 S/cm of PGE without P25, and the diffusion coefficient was also increased by 19.0 %. Different photoelectrodes were obtained by using three kinds of surfactants (cetylamine, octadecylamine, and P123) as pore-forming materials, and their morphologies were contrasted through scanning electron microscopy (SEM). The results showed that gel electrolyte can increase the short-circuit current density ( J
sc ) from 11.01 to 12.99 mA/cm 2 in DSSCs. Moreover, unlike the liquid electrolyte, the gel electrolyte is more conducive to the TiO 2 photoelectrodes with larger pores. In conclusion, the efficiency of DSSC with gel electrolyte and P123 as pore-forming material was 6.73 %, which was 12 % higher than the liquid electrolyte in the same test condition. In addition, the sealed gel electrolyte DSSCs showed better stability than did liquid electrolyte DSSCs during nearly 600 h.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-14
·
IF 10.6
Li Yuhuan,Wang Xin,Gao Yu,Zhang Ziyi,Liu Te,Zhang Zhuo,Wang Yinan,Chang Fei,Yang Modi
Rheumatoid arthritis (RA) involves chronic inflammation, oxidative stress, and complex immune cell interactions, leading to joint destruction. Traditional treatments are often limited by off-target effects and systemic toxicity. This study introduces a novel therapeutic approach using hyaluronic acid (HA)-conjugated, redox-responsive polyamino acid nanogels (HA-NG) to deliver tacrolimus (TAC) specifically to inflamed joints. The nanogels’ disulfide bonds enable controlled TAC release in response to high intracellular glutathione (GSH) levels in activated macrophages, prevalent in RA-affected tissues. In vitro results demonstrated that HA-NG/TAC significantly reduced TAC toxicity to normal macrophages and showed high biocompatibility. In vivo, HA-NG/TAC accumulated more in inflamed joints compared to non-targeted NG/TAC, enhancing therapeutic efficacy and minimizing side effects. Therapeutic evaluation in collagen-induced arthritis (CIA) mice revealed HA-NG/TAC substantially reduced paw swelling, arthritis scores, synovial inflammation, and bone erosion while suppressing pro-inflammatory cytokine levels. These findings suggest that HA-NG/TAC represents a promising targeted drug delivery system for RA, offering potential for more effective and safer clinical applications.
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高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2024
·Vol. 42
·期 5
·579-590
·
IF 4.1
Li Xiao-Di,Li Shu-Sheng,Jiang Xu-Bao,Zhu Xiao-Li,Kong Xiang Zheng
Owing to their high significance in fundamental study and diverse applications, stimuli-responsive and fluorescent polymers, particularly those with cluster-triggered emission (CTE) featured by non-conjugated chromophores, have drawn tremendous attention in recent years. In this work, fluorescent and multi-responsive polysiloxane (FRPS) was synthesized by hydrolytic condensation polymerization of 3-aminopropyl methyl diethoxysilane (APMS) with 3-( N -isopropyl propionamide) iminopropyl methyl diethoxysilane (APMS-NIP), which was formed in situ through aza-Michael addition between APMS and N -isopropyl acrylamide. FRPS was not only highly sensitive to temperature, pH and CO 2 in water, but also showed an enhanced and stimuli-adjustable fluorescence emission. The effects of monomer feeding, pH and CO 2 on its lower critical solution temperature and fluorescent property were investigated. FRPS fluorescence emission was ascribed to CTE mechanism. In addition, FRPS was shown to be highly potential as physiological indicator for cell imaging, and for controlled release and trace detection of doxorubicin. This study provides therefore a type of stimuli-responsive and fluorescent material for potential applications in biomedical fields, and it is also of great significance for understanding of the fluorescence mechanism of polysiloxane-based stimuli-responsive polymers.
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高影响因子
IONICS
·
2020
·Vol. 26
·期 11
·5471-5480
·
IF 2.394
Pan Fusheng,Qiao Lina,Yuan Biao,Duan Cuijia,Wang Jianjie,Wu Wei,Lin Quanfan,Shi Yake,Chen Zan,Jiang Zhongyi
Poly(m-phenylene isophthalamide) (PMIA) membranes are promising heat-tolerant separator candidates of lithium-ion batteries (LIBs), while their wettability toward carbonate electrolyte and corresponding batteries performance are not desirable. Herein, PMIA membranes coated with ultrathin mussel-inspired polydopamine (PDA) layer were fabricated as separators in LIBs. The introduced amino, quinonyl, and catechol groups in PDA coating could enhance the wettability of the resultant PMIA-PDA separator toward electrolyte. The PMIA-PDA separator had excellent thermal stability, showing a thermal shrinkage of 5.2% at 200 °C. The contact angle of the thermal-treated PMIA-PDA separator could maintain at 26.1°, which decreased by 42.7% compared with the pristine PMIA separator. The PMIA-PDA separator also displayed a high ionic conductivity (0.86 mS cm −1 ) and a low interfacial electric resistance (62.4 Ω). Consequently, the assembled LiCoO 2 /PMIA-PDA/Li battery achieved good cyclic stability with the capacity retention of 84.3% (121.1 mAh g −1 ) after 100 cycles under 30 °C. More significantly, the relevant capacity retention remained at 89.0% (127.5 mAh g −1 ) under 60 °C, which implied a great potential of PMIA-PDA membranes as separators of high-safety LIBs.
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