Foods
·
2024
·Vol. 13
·期 10
·1573
·
IF 4.7
Shufen Wu,Changyan Dong,Meihui Zhang,Yi Cheng,Xiaobo Cao,Benxu Yang,Chao Li,Xin Peng
Red yeast rice dietary supplements have been proven to ameliorate hyperglycemia, but the mechanism was unclear. In this work, ankaflavin (AK) and monascin (MS), as typical pigments derived from red yeast rice, were found to exert noteworthy inhibitory ability against α-glucosidase, with an IC50of 126.5 ± 2.5 and 302.6 ± 2.5 μM, respectively, compared with acarbose (IC50= 341.3 ± 13.6 μM). They also exhibited mixed-type inhibition of α-glucosidase in vitro and caused fluorescence quenching through the static-quenching process. Molecular-docking studies indicated that AK and MS bind to amino acid residues outside the catalytic center, which induces structural changes in the enzyme, thus influencing its catalytic activity. The anti-glycation ability ofMonascus-fermented products was evaluated, and they exhibited a high inhibition rate of 87.1% in fluorescent advanced glycation end-product formation at a concentration of 0.2 mg mL−1, while aminoguanidine showed a rate of 75.7% at the same concentration. These results will be significant in broadening the application scope ofMonascuspigments, especially AK and MS, in treating type 2 diabetes.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Han Lv,Zhicheng Cui,Qi Zhao,Chaofan Zhang,Bin Cui,Dandan Zhou
Both iron-reducing bacteria (IRB) and iron are loaded to anode during the microbial electro-Fenton (MEF) system, which simultaneously achieves the refractory wastewater treatment and iron circulation. Meanwhile, the amounts of iron play a pivotal role in both performance of microbial electrochemical system (MES) and regulation of the microbial community and metabolism. Therefore, additional α-Fe 2 O 3 was adopted in MEF system, and then the regulation mechanism for anodic microorganism was thoroughly revealed in this study. When α-Fe 2 O 3 loading increased to 2 mg/cm 2 , phenol removal rate improved by 3.8 times and the anaerobic biofilm thickness of 1000 μm on the anode surface; following, current density increased by 96.2 % and current resistance reduced by 50.5 %. Metatranscriptomic sequencing indicated that α-Fe 2 O 3 could improve microbial metabolism activity via up-regulating the enzymes associated with tricarboxylic acid cycle (TCA cycle). Meanwhile, Shewanella abundance increased 326-fold, and α-Fe 2 O 3 up-regulated the expression of Type II secretion system and cytochrome c synthesis, promoting extracellular electron transfer (EET) process via direct contact pathway. Overall, this study provided a novel strategy for optimizing the performance of MEF system.
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JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Yu Tian,Haodi Yang,Fei Wang,Ping Ning,Kunlin Li,Kai Li
Increasing the oxygen vacancy (Ov) content and inducing lattice oxygen activation are critical for enhancing the oxidation performance of transition metal oxides. However, precisely regulating these properties remains a significant challenge. In this study, a stepwise pyrolysis method was employed on a colloidal complex formed by citric acid coordinated with Fe(NO 3 ) 3 ·9H 2 O to synthesize Fe 2 O 3 -X catalysts comprising a mixture of α- and γ-Fe 2 O 3 characterized by abundant surface oxygen vacancies and activated lattice oxygen. The resulting Fe 2 O 3 -1 presented excellent low-temperature H 2 S selective catalytic oxidation (H 2 S-SCO) performance, achieving selective oxidation of H 2 S at 130°C with removal efficiency and sulfur selectivity exceeding 95 % and 85 %, respectively, within 60 h. Characterization results show that the γ-Fe 2 O 3 induced by citric acid forms a crystal interface with the α-Fe 2 O 3 . At the interface, the asymmetrically coordinated lattice oxygen is more likely to be released from the catalyst, thereby enhancing the oxidation ability of the catalyst. Furthermore, the synthesis method effectively modulates the catalyst’s surface basicity, resulting in an optimal distribution of basic sites that mitigates catalyst poisoning and deactivation. This study presents a straightforward approach for synthesizing Fe 2 O 3 catalysts with enhanced oxidation ability, offering valuable insights for developing more efficient transition metal catalysts.
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POLYMER
·
2025
·
IF 4.5
Pengkai Qin,Linbo Wu,Suyun Jie
Two series of high molecular weight aliphatic-aromatic random copolyesters: PBXT, where X represents the carbon atom number of the aliphatic α,ω -diacids, PBXT 48 and PBXT 50w (X = 4–14, even), with molar and mass copolymer compositions (48 mol% and 50 wt%) equal to those of commercial poly(butylene adipate-co-terephthalate) (PBAT) were synthesized from butanediol, terephthalic acid and various aliphatic α , ω -diacids. They were characterized with 1 H NMR, and their melt crystallization, optical, mechanical and gas barrier properties were assessed. In these copolyesters, the melt crystallization of butylene terephthalate (BT) sequence is dominant, while the butylene adipate (BX) sequences can also crystallize from melt when the BX content is high enough, the chain length of α , ω -diacid unit or the crystallization time is long enough. Long enough chain length of α , ω -diacid unit ( X ≥ 6) endows PBXT with sufficient chain flexibility/mobility and, therefore, excellent melt crystallizability at rapid cooling. For PBXTs with X ≥ 6, the effect of X on BT crystallization is not monotonic. In particular, both PB6T and PB12T displayed a higher melt crystallization temperature than the adjacent PBXTs. Compared to PB6T, PB4T has higher transparency and gas barrier properties; with increasing X from 6 to 14, the PBXTs show increased transparency and water vapor barrier, but decreased oxygen barrier performance. The tensile modulus and strength of PBXT decrease, and the elongation at break increases with increasing X . But the effect of X is clearly weakened for PBXTs with the same mass composition of 50 wt%.
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Toxins
·
2021
·Vol. 13
·期 5
·343
·
IF 4.546
Xudong Luo,Li Ding,Xiangdong Ye,Wen Zhu,Kaiyue Zhang,Fangyan Li,Huiwen Jiang,Zhiwen Zhao,Zongyun Chen
Scorpion venoms are rich resources of antimicrobial peptides (AMPs). While the short-chain noncysteine-containing AMPs have attracted much attention as templates for drug development, the antimicrobial potential of long-chain noncysteine-containing AMPs has been largely overlooked. Here, by using the online HeliQuest server, we designed and analyzed a series of 14-residue fragments of Smp43, a 43-residue long-chain noncysteine-containing AMP identified from the venom ofScorpio maurus palmatus. We found that Smp43(1-14) shows high antimicrobial activity against both Gram-positive and Gram-negative bacteria and is nontoxic to mammalian cells at the antimicrobial dosage. Sequence alignments showed that the designed Smp43(1-14) displays a unique primary structure that is different from other natural short-chain noncysteine-containing AMPs from scorpions, such as Uy17, Uy192 and IsCT. Moreover, the peptide Smp43(1-14) caused concentration-dependent fluorescence increases in the bacteria for all of the tested dyes, propidium iodide, SYTOXTMGreen and DiSC3-5, suggesting that the peptide may kill the bacteria through the formation of pore structures in the plasma membrane. Taken together, our work sheds light on a new avenue for the design of novel short-chain noncysteine-containing AMPs and provides a good peptide template with a unique sequence for the development of novel drugs for use against bacterial infectious diseases.Keywords:scorpion venom;noncysteine-containing AMPs;Smp43;Smp43(1-14);antimicrobial resistance;HeliQuestKey Contribution:Smp43(1-14), a short fragmented peptide of Smp43, was designed as a novel antimicrobial peptide with a unique sequence and potent antimicrobial activity.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Yinshuo Xu,Yuanjie Shan,Yuta Nishio,Kenichiro Todoroki,Toshimasa Toyo'oka,Toufeng Jin,Jun Zhe Min
With the development of untargeted metabolomics, studies have shown that alterations in the serum levels of specific amino acids are strongly correlated with the development of early-stage colorectal cancer (CRC). However, the relationship between optically active free amino acids in human nails and the risk of CRC remains unclear. In this study, a highly sensitive UHPLC-MS/MS chiral separation method based on ( S )-(−)-DBD-Pro-COCl derivatization was established for the simultaneous detection of DL-Asp, DL-Kyn, DL-α-hydroxybutyric acid (DL-α-HB), and Cystamine in the fingernails of patients with CRC. The derivatization reagents were incubated with DL-amino acids, DL-α-HB and Cyst at 60 °C for 15 min to form diastereomers. Subsequently, an Imtakt cadenza CD-C 18 column (3.0 × 150 mm, 3.0 μm) was used for the separation of diastereomers under gradient elution of 10 mM HCOONH 4 in H 2 O as mobile phase A and 0.1 % HCOOH in CH 3 CN as mobile phase B. The degrees of separation (Rs) ranged from 1.76 to 3.21. R 2 was >0.9995, indicating good linearity; the limit of detection (LOD) was 25–100 fmol; the interday and intraday precision of DL-Asp and DL-α-HB detection were 0.85 %–4.91 %; and the average recovery ranged from 81.12 % to 110.2 %. However, during the determination process of fingernail samples, only DL-Asp and DL-α-HB were successfully detected, while DL-Kyn and Cyst were not detected. Therefore, this novel method was used to quantify DL-Asp and DL-α-HB in fingernail samples from 32 healthy volunteers (HVs) and 16 patients with CRC (CPs). The results revealed significant differences in D-Asp ( p < 0.05), L-Asp ( p < 0.01), and L-α-HB ( p < 0.05) levels between male HVs and CPs and in D-α-HB ( p < 0.05) levels between female HVs and CPs. In addition, the D/L-α-HB ratio ( p < 0.01) was significantly different between male HVs and CPs. These findings validate the use of DL-amino acids, DL-α-HB and Cyst from human fingernails as potential diagnostic biomarkers for CRC, providing a basis for the clinical application of human fingernail samples for the noninvasive diagnosis of CRC.
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International Journal of Peptide Research and Therapeutics
·
2020
·Vol. 27
·期 1
·527-542
·
IF 1.5
Zhu Jie,Huang Yibing,Hu Cuihua,Huang Yuxi,Chen Mingxia,He Xu,Zhang Yanan,Wang Yiou,Chen Yuxin
Drug resistance is an increasing problem in the treatment of vaginitis bacterial vaginosis and vulvovaginal candidiasis caused by biofilm-forming bacteria and fungi. In this study, the antimicrobial activities of the synergistic effects coordinated by antimicrobial peptides HPRP-A1, HPRP-A2, and chlorhexidine acetate (CHA), against biofilms caused by Gram-negative and Gram-positive bacteria, and one fungus Candida albicans , were investigated in vitro and in mouse and rat infection models. The minimal biofilm inhibitory concentration and the fractional biofilm inhibitory concentration of HPRP-A1, HPRP-A2 alone and in combination with CHA were determined by classical methods. The crystal violet staining assay was used to determine the biomass of agents on biofilms. The MTT assay was used to determine the metabolic activity of agents on biofilms. Different microscopy assays were used to observe the morphological characteristics of bacterial and fungal biofilms after drug treatments. The extracellular polysaccharide was detected by a phenol–sulfuric acid method. The combined uses of HPRP-A1, HPRP-A2 and CHA exerted strong synergistic effects to inhibit the formation of bacterial and fungal biofilms and disrupt the construction of biofilms in vitro and in vivo. In animal vaginitis models, the drug combination reduced 99.9% of the number of bacterial and fungal CFU, and showed significant therapeutic effects against biofilm vaginitis. The mechanism of membrane disruption coordinated by the combination of drugs depends not only on the inhibition of extracellular polysaccharides secreted by bacterial and fungal cells, but also on the ability of the peptides to penetrate the cell membrane.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Vemana Gowd,Naymul Karim,Lianghua Xie,Mohammad Rezaul Islam Shishir,Yang Xu,Wei Chen
Polysaccharides and fruit extracts are applied in dairy products to enhance their nutritional property, but the effects of such formulations on the functions and biological activities are yet to be explored. Therefore, this study was aimed at evaluating the effect of interactions among milk protein (beta-lactoglobulin; BLG), polysaccharides (pectin, P; chitosan, CH), and anthocyanin (pelargonidin-3- O -glucoside; P3G) in improving the bioavailability and biological activity of P3G. After gastrointestinal digestion (GID), the content of free P3G in different model solutions were as follows: P3G-alone (73.59 μg/mL), P3G-P (66.59 μg/mL), P3G-CH (36.72 μg/mL), P3G-BLG (64.92 μg/mL), P3G-P-BLG (64.92 μg/mL), and P3G-CH-BLG (39.61 μg/mL). Less amount of free P3G in model solutions indicated increased complex formation of P3G with protein and/or polysaccharides during GID. These complexes resulted in protection and progressive release of P3G in the gastrointestinal tract. Chitosan exhibited more protection to P3G compared with P and BLG. In addition, α-glucosidase inhibitory activity and ROS scavenging activities of conjugated-P3G samples were potentially augmented after GID. However, the presence of polysaccharides and protein in the model solutions did not show any negative effect on the biological activity of P3G. Thus, pure P3G can be used as a nutritional ingredient in dairy industries.
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Biomolecules
·
2025
·Vol. 16
·期 1
·68
·
IF 4.8
Bin-Chun Li,Weijuan Dong,Bingbing Wu,Yanlong Liu,Na Han,Guo-Bin Ding
α-L-Rhamnosidase can specifically hydrolyze plant natural glycosides and holds significant potential for biocatalytic applications in functional foods, healthy products, and pharmaceutical industries. Herein, a novel thermophilic and acidophilic α-L-rhamnosidase TsRha fromThermotogasp. 2812B belonging to glycoside hydrolase family 78 was identified by genome mining and comprehensively characterized by bioinformatics, computer-aided structural analysis, and biochemical characterization. TsRha possesses a domain architecture comprising one catalytic (α/α)6-barrel domain and four β-sheet domains. TsRha displayed optimal activity at 90 °C and pH 5.0, remarkable thermostability at 80 °C, and considerable tolerance to organic solvents. TsRha exhibited broad substrate selectivity and might efficiently hydrolyze a series of natural flavonoid glycosides with various glycosidic bonds (α-1, α-1, 2, α-1, 6) from different aglycone subgroups (flavanone, flavone, flavonol, and dihydrochalcone). Moreover, it demonstrated high conversion efficiencies toward a variety of natural flavonoid diglycosides rutin, naringin, naringin dihydrochalcone, hesperidin, and troxerutin, achieving ≥99.1% conversion within 20~100 min. The excellent properties including high activity, thermophilicity, acidophilicity, good thermostability, broad substrate spectrum will make the α-L-rhamnosidase TsRha a promising biocatalyst for the efficient production of rare and high-value flavonoid glucosides with improved bioavailability and bioactivity.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Yajuan Qin,Xiaoai Chen,Fei Xu,Chunhe Gu,Kexue Zhu,Yanjun Zhang,Gang Wu,Ping Wang,Lehe Tan
The structure–activity relationship and inhibitory mechanism of flavonols on α-glucosidase were studied by inhibition kinetics, multispectral study, and molecular docking. The flavonols of rutin, quercetin and kaempferol effectively inhibit the activity of α-glucosidase, among which quercetin and rutin showed the strongest and weakest inhibitory abilities, respectively. The inhibitory ability of flavonols was enhanced by hydroxylation at C3′ of B ring, while it was weakened by diglycosylation at C3 of C ring. Remarkably, the quenching affinity and inhibitory ability of flavonols were inconsistent, which was different from the conclusions reported by some previous studies. This may be ascribed to the hydroxyl groups of C3′ of B ring and C3 of C ring. Furthermore, three flavonols were spontaneously bound to α-glucosidase through hydrophobic interactions and hydrogen bonding, which caused the structure and hydrophobic microenvironment of α-glucosidase to change, resulting in significant inhibition of α-glucosidase by flavonols.
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Reaction Kinetics Mechanisms and Catalysis
·
2024
·Vol. 137
·期 3
·1635-1649
·
IF 1.7
Pan Weixing,Chen Hui,Chen Aicheng
A series of low-cost α-Ni(OH) 2 /SiO 2 catalysts were successfully fabricated by the vapor-induced internal hydrolysis method, then the hydrogenation of 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF) was investigated by using the ethanol as the hydrogen donor over these as-prepared α-Ni(OH) 2 /SiO 2 catalysts. Based on the reaction results, these as-prepared catalysts, which could dispense with extra pre-reduction treatment before reactions, exhibited superior catalytic performance for the HMF hydrogenation to BHMF. A remarkable intrinsic selectivity of BHMF selectivity of almost 100% with about 90% HMF conversion could be obtained under 195 °C for 5 h in an N 2 atmosphere over the α-Ni(OH) 2 /SiO 2 catalyst of 5-Ni(OH) 2 /SiO 2 with about 5 wt% Ni on SiO 2 . The almost constant HMF conversion and BHMF intrinsic selectivity found from the recycling tests can indicate that the catalyst was stable without the obvious loss of its catalytic activity after recycling. Moreover, the mechanism of HMF hydrogenation using ethanol over the α-Ni(OH) 2 /SiO 2 catalysts could be referred to as the mechanism of the Meerwein-Ponndorf-Verley reaction. This work will provide a simple synthesis method for future design and development of high-performance, low-cost, and low-energy consumption catalysts for HMF hydrogenation to BHMF.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lijun Yang,Tiantian Zhou,Zhiyi Zhang,Wei Qi,Mengfan Wang
The enzymatic oxidation of amino acids using amino acid oxidases (AAOs) as biocatalysts for α-keto acid synthesis has garnered increasing attention due to its environmental friendliness and exceptional reaction efficiency. However, the by-product H 2 O 2 inevitably causes the decarboxylation of α-keto acids and subsequently deactivates the enzyme. Herein, we used a simple in-situ biomineralization method to immobilize Pt NPs with catalase-like activity and L-amino acid oxidase (LAAO) in to a hydrogen-bonded organic framework ( TW HOF) under a mild condition, and the synthesized LAAO-Pt@ TW HOF cascade catalytic system was applied to synthesis of α-keto acid. Compared with free LAAO, the LAAO-Pt@ TW HOF exhibited a 3.3-fold activity, well reusability and complete conversion (100 %) of L-tryptophan to indole-3-pyruvic acid was achieved. This work offers a potential application of HOF materials in constructing high-efficiency enzyme-metal coupled catalyst for cascade reactions.
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