LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Yujie Chen,Ruhui Liu,Jingyan Li,Yanjie Wang,Chunfeng Guo,Xin Lü
1-Oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) is the most abundant triacylglycerol (TAG) molecule in Chinese human milk. The selectivity of three lipases including Rhizomucor miehei lipase (RML), Thermomyces lanuginose lipase (TL), and Candida antarctica lipase (CALB) in OPL synthesis were evaluated. The effects of different reaction conditions on selectivity were first investigated. Unexpectedly, the three sn -1,3 regioselectivity lipases, also have selectivity for the structure of product TAGs. Compared with symmetric TAGs, lipases had poor selectivity toward asymmetric OPL. Furthermore, molecular simulations were used to deeply understand the molecular factors affecting lipase selectivity. The results showed that hydrophobic interactions, lipases lid domain flexibility, and the stability of hydrogen bonds between lipases and TAGs were the main molecular factors responsible for the selectivity of lipases.
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ACS Chemical Neuroscience
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2023
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IF 5
Hang Zhou,Zhang Mao,Xiaonan Zhang,Ruomiao Li,Jian Yin,Yinghui Xu
Parkinson’s disease (PD), known as a neurodegenerative disease, is characterized by movement disorders, with increasing age being the predominant risk factor for its development. Mangiferin, a bioactive compound isolated from mango, shows potent neuroprotection. In our work, we investigated the neuroprotection and mechanisms of mangiferin against PD. We established PD models by treating SH-SY5Y cells with rotenone and mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and investigated the therapeutic effects of mangiferin. Our results showed that mangiferin exhibited a cell-protective effect. Mangiferin also improved the motor behavior and attenuated the activation of microglia and astrocytes in MPTP mice. In addition, mangiferin decreased reactive oxygen species (ROS) levels and increased glutathione (GSH) and superoxide dismutase (SOD). Mangiferin also markedly activated GIT1, p-ERK, Nrf2, HO-1, and SOD expression and inhibited Keap1 expression in vitro and in vivo. To further investigate the role of GIT1, GIT1 siRNA was used. In the presence of GIT1 siRNA, the neuroprotection of mangiferin in PD was weakened. Our results indicate that mangiferin exhibited its therapeutic effect against PD by regulating GIT1 and its downstream Keap1/Nrf2 pathways. Our studies exhibited that mangiferin showed neuroprotection in PD, and its main target was GIT1. What is more, mangiferin could reduce the oxidative stress of PD by targeting GIT1 and its downstream Keap1/Nrf2 pathways. These indicated that mangiferin is a good candidate for PD therapy. However, the role of p-ERK in mangiferin-treated PD requires further investigation.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
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IF 8.6
Jiong Wang,Zhibin Liu,Zhirong Sun
In this study, a graphite felt (GF) electrode material supporting a Cu-based catalyst (CuHDC-400/GF) was prepared by a hydrothermal process and subsequent heat treatment. A heterogeneous electro-Fenton (EF) system was built to investigate the degradation of sulfamethoxazole (SMX) by the composite cathode. Scanning electron microscopy, X-ray diffraction, Raman spectroscopy , and X-ray photoelectron spectroscopy were used to characterize the physical and chemical properties of the composite cathode. The results show that the composite cathode CuHDC-400/GF has excellent catalytic performance, achieving 100 % removal of SMX (10 mg/L) within 45 min (aeration rate = 0.6 L min −1 , current density = 5 mA cm −2 , initial pH = 5.6). Furthermore, efficient removal of SMX can be achieved over a wide pH range (pH = 3–9). The electrode material can still efficiently degrade SMX after eight cycles. Compared with a heterogeneous EF system, in which the Cu catalyst was directly added to the reaction solution, using CuHDC-400/GF as the cathode realizes the in-situ catalysis of H 2 O 2 , leading to reduced catalyst consumption and enhanced electrode stability. The investigation of potential SMX degradation pathways and mechanisms revealed that the continuous production of active free radicals is facilitated by the redox cycle between Cu Ⅰ and Cu Ⅱ on the electrode surface, which can enable SMX degradation. Unlike the Fe-based Fenton reaction, the active superoxide radical O 2 – was identified as the major contributor to SMX degradation in this system, followed by the hydroxyl radical OH. The active sites for SMX degradation were predicted by density functional theory calculations. Combined with the measured mass/charge ratios, the possible degradation paths and intermediates of SMX were predicted. Furthermore, toxicity analysis shows that toxicity significantly declines after degradation. This work is expected to inspire subsequent research on heterogeneous EF cathode materials with high catalytic performance.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Yuanjie Teng,Zhenni Wang,Shaohua Zuo,Xin Li,Yinxin Chen
Universal and fast antibiotic residues detection technology is imperative for the control of food safety in aquatic products. However, accurate surface-enhanced Raman scattering (SERS) quantitative detection of complicated samples is still a challenge. A recognition method powered by deep learning and took advantage of the unique fingerprint information merits of SERS was proposed. Herein, the spectra were collected by Ag nanofilm SERS substrate prepared by self-assembly of Ag nanoparticles on water/oil interface. A SERS-based database of commonly used antibiotics in aquatic products was set up, which is suitable for employed as input data for learning and training. The results show that the five types of antibiotics are successfully distinguished through principal component analysis (PCA) and each antibiotic in every type was successfully distinguished. Furthermore, one-dimensional convolutional neural networks (1-D CNN) was used to distinguish the antibiotics, and the results show that all the test samples were correctly predicted by 1-D CNN model. The results of this research suggest the great potential of the combination of SERS spectra with deep learning as a method for rapid and highly accurate identification of antibiotic residues in aquatic products.
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NEW JOURNAL OF CHEMISTRY
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2022
·Vol. 47
·期 5
·2257-2263
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IF 3.925
Shiyu Nie,Fengfeng Chen,Tianbo Shen,Tingting Wang,Ningyu Di,Junkuo Gao
Adjusting the internal porous environment of MOFs to enhance their adsorption properties is considered an effective strategy to improve their gas separation performance. Herein, 1-ethyl-3-methyl-imidazolium acetate ([Emim][Ac]) was encapsulated in UiO-66 by an impregnation method for the improvement of carbon dioxide (CO2) capture performance. The synthesis and detailed characterizations of the developed [Emim][Ac]@UiO-66 are reported, and the effect of the loading amount of [Emim][Ac] on the CO2 separation performance was also studied. The obtained [Emim][Ac]@UiO-66 could efficiently show the synergistic effect of the adsorption of CO2 between [Emim][Ac] and UiO-66. The optimized [Emim][Ac]@UiO-66 exhibited a much higher CO2 adsorption capacity at 298 K and 1.0 bar, affording 2 times the CO2 uptake than its original UiO-66. In addition, the CO2/N2 selectivity and CO2/CH4 selectivity of [Emim][Ac]@UiO-66-3.5% are significantly higher than those of UiO-66. This study for designing various MOF-encapsulated ionic liquids potentially paves the way for the development of highly efficient absorbents for gas separations.
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Nanoscale
·
2023
·Vol. 15
·期 8
·4063-4070
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IF 6.7
Mengjiao Li,Qingjie Yu,Meie Zheng,Rongrong Jiang,Hongda Zhu,Huiling Guo,Hongmei Sun,Mingxing Liu
A label-free electrochemical immunosensor for high-sensitive detection of β-amyloid 1–42 (Aβ 1–42) was constructed based on Au-modified B, S, and N co-doped reduced graphene oxide (Au-BSN-rGO). The electronic structure of Au-BSN-rGO was investigated by first-principles calculations, which showed that the band gap of graphene was opened, thus improving its electrical conductivity. Moreover, Au-BSN-rGO was successfully prepared and characterized, and the obtained results discovered that it could be used as a signal amplifier for immunosensors due to the advantages of the good electrochemical characteristics and enormous surface area of BSN-rGO and the accelerated electron transfer ability of Au NPs. Furthermore, the label-free electrochemical immunosensor had a linear detection range of 0.1 pg mL−1–10 ng mL−1 and a detection limit of 0.072 pg mL−1, and it had good specificity, stability, and reproducibility. Also, this immunosensor showed recoveries of 89%–109% with an RSD of 2.61%–4.19% for detecting Aβ 1–42 in actual sample analysis. Therefore, the label-free electrochemical immunosensor based on Au-BSN-rGO should have a promising clinical application prospect for detecting Aβ 1–42.
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ACS Nano
·
2022
·
IF 18.027
Hanyu Wu,Hairui Huang,Yizhou Zhang,Xinglin Lu,Pawel W. Majewski,Xunda Feng
Soft materials with self-assembled networks possess saddle-shaped interfaces with distributed negative Gaussian curvatures. The ability to stabilize such a geometry is critically important for various applications but can be challenging due to the possibly “deficient” packing of the building blocks. This nontrivial challenge has been manifested, for example, by the limited availability of cross-linkable bicontinuous cubic (Q) liquid crystals (LCs), which can be utilized to fabricate compelling polymers with networked nanochannels uniformly sized at ∼1 nm. Here, we devise a facile approach to stabilizing cross-linkable Q mesophases by leveraging the synergistic self-assembly from pairs of scalably synthesized polymerizable amphiphiles. Hybridization of the molecular geometries by mixing significantly increases the propensity of the local deviations in the interfacial curvature specifically required for Q assemblies. “Normal” (type 1) double gyroid LCs possessing 1 nm ionic channels conforming to minimal surfaces can be formulated by simultaneous hydration of the amphiphile mixtures, as opposed to the formation of hexagonal or lamellar mesophases exhibited by the single-amphiphile systems, respectively. Fixation of the bicontinuous network in polymers via radical polymerization has been efficaciously facilitated by the presence of the bifunctional polymerizable groups in one of the employed amphiphiles. High-fidelity lock-in of the ordered continuous 1 nm channels has been unambiguously confirmed by the observation of single-crystal-like diffraction patterns from synchrotron small-angle X-ray scattering and large-area periodicities by transmission electron microscopy. The produced polymeric materials exhibit the required mechanical integrity as well as chemical robustness in a variety of organic solvents that benefit their practical applications for selective transport of ions and molecules.
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JOURNAL OF CHEMICAL THERMODYNAMICS
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2023
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IF 2.6
Mingcheng Li,Xiaojie Liu,Chunying Zhu,Taotao Fu,Xiqun Gao,Youguang Ma
Densities and viscosities were measured for binary solutions MEA (monoethanolamine) + 3DMA1P (3-dimethylamino-1-propanol), 3DMA1P + H 2 O and ternary solutions MEA + 3DMA1P + H 2 O at P = 0.101 MPa, T = 293.15 K ∼ 333.15 K. Based on the experimental densities of solutions, the volume properties of the excess molar volume, partial molar volume, apparent molar volume and limiting partial molar volume were calculated. Moreover, the viscosity deviation and viscous flow activation energy were calculated based on the experimental viscosity. Additionally, the intermolecular interactions of the mixed solutions were analyzed and discussed, and the Redlich-Kister equation was used to correlate the excess molar volume and viscosity deviation of the binary solutions.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Ziqi Zhao,Shaokun Liang,Chengjun Kang,Zhaoqiang Zhang,Liqin Zhou,Zhenxia Zhao,Xingzhen Qin,Yushan Pang,Hongbing Ji,Kungang Chai
Adsorption is considered as a promising method to purify the mixed acetophenone (AP) and 1-phenylethanol (1-PE) that are generally difficult to separate. Many efforts have been made to improve the preferential uptake of AP over 1-PE in aqueous phase, but there exists a practical bottleneck associated with their limited aqueous solubility. Herein, we demonstrate unprecedented reversal of selectivity in the organic medium enabled by β-ketoenamine-linked covalent organic frameworks (COFs), which were fabricated successfully by polycondensation between 1,3,5-triformylphloroglucinol (Tp) and diamines. The dependence of selective adsorption ability toward 1-PE on pore size was firstly investigated experimentally, indicating that TpBZ-COF with moderate pore size outperformed other analogues. The adsorption behaviors of 1-PE and AP onto TpBZ-COF were examined systematically. The evaluation results revealed a strong affinity toward 1-PE versus AP as well as excellent stability in recycling experiments. Further, the dynamic breakthrough experiments confirmed the practical application potential and remarkable recyclability. To probe the mechanism and structure–activity relationship, an imine-linked COF possessing similar porous structure was used as a reference for comparison of separation performance both experimentally and theoretically. The occurrence of hydrogen bonding between the hydroxyl of 1-PE and β-ketoenamine-linkage as H-bond acceptor in suitable pore spaces endows TpBZ-COF a fantastic candidate for purifying 1-PE and AP from petrochemical by-products.
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CNS Neuroscience & Therapeutics
·
2023
·
IF 5.5
Tao Zhang,Yuan Tian,Xiaoqing Zheng,Ruomeng Li,Li Hu,Xindong Shui,Yingxue Mei,Quling Wang,Mi Zhang,Xiuzhi Zheng,Long Wang,Dongmei Chen,Wucheng Tao,Tae Ho Lee
Aims The autophagy-lysosomal pathway is important for maintaining cellular proteostasis, while dysfunction of this pathway has been suggested to drive the aberrant intraneuronal accumulation of tau protein, leading to synaptic damage and cognitive impairment. Previous studies have demonstrated that the activation of transient receptor potential vanilloid 1 (TRPV1) by capsaicin has a positive impact on cognition and AD-related biomarkers. However, the effect and mechanism of TPRV1 activation on neuronal tau homeostasis remain elusive. Methods A mouse model of tauopathy was established by overexpressing full-length human tau in the CA3 area. Mice were fed capsaicin diet (0.0125%) or normal diet for 9 weeks. The cognitive ability, synaptic function, tau phosphorylation levels, and autophagy markers were detected. In vitro, capsaicin-induced alterations in cellular autophagy and tau degradation were characterized using two cell models. Besides, various inhibitors were applied to validate the role of TRPV1-mediated autophagy enhancement in tau clearance. Results We observed that TRPV1 activation by capsaicin effectively mitigates hippocampal tau accumulation-induced synaptic damages, gliosis, and cognitive impairment in vivo. Capsaicin promotes the degradation of abnormally accumulated tau through enhancing autophagic function in neurons, which is dependent on TRPV1-mediated activation of AMP-activated protein kinase (AMPK) and subsequent inhibition of the mammalian target of rapamycin (mTOR). Blocking AMPK activation abolishes capsaicin-induced autophagy enhancement and tau degradation in neurons. Conclusion Our findings reveal that capsaicin-induced TRPV1 activation confers neuroprotection by restoring neuronal tau homeostasis via modulating cellular autophagy and provides additional evidence to support the potential of TRPV1 as a therapeutic target for tauopathies.
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Journal of Pharmaceutical Analysis
·
2023
·
IF 8.8
Zhang Mao,Haochen Hui,Xuerong Zhao,Lina Xu,Yan Qi,Lianhong Yin,Liping Qu,Lan Han,Jinyong Peng
It is necessary to explore potent therapeutic agents via regulating gut microbiota and metabolism to combat Parkinson's disease (PD). Dioscin, a bioactive steroidal saponin, shows various activities. However, its effects and mechanisms against PD are limited. In this study, dioscin dramatically alleviated neuroinflammation and oxidative stress, and restored the disorders of mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). 16S rDNA sequencing assay demonstrated that dioscin reversed MPTP-induced gut dysbiosis to decrease Firmicutes -to- Bacteroidetes ratio and the abundances of Enterococcus , Streptococcus , Bacteroides and Lactobacillus genera , which further inhibited bile salt hydrolase (BSH) activity and blocked bile acid (BA) deconjugation. Fecal microbiome transplantation test showed that the anti-PD effect of dioscin was gut microbiota-dependent. In addition, non-targeted fecal metabolomics assays revealed many differential metabolites in adjusting steroid biosynthesis and primary bile acid biosynthesis. Moreover, targeted bile acid metabolomics assay indicated that dioscin increased the levels of ursodeoxycholic acid, tauroursodeoxycholic acid, taurodeoxycholic acid and β-muricholic acid in feces and serum. In addition, ursodeoxycholic acid administration markedly improved the protective effects of dioscin against PD in mice. Mechanistic test indicated that dioscin significantly up-regulated the levels of takeda G protein-coupled receptor 5 (TGR5), glucagon-like peptide-1 receptor (GLP-1R), GLP-1, superoxide dismutase (SOD), and down-regulated NADPH oxidases 2 (NOX2) and nuclear factor-kappaB (NF-κB) levels. Our data indicated that dioscin ameliorated PD phenotype by restoring gut dysbiosis and regulating bile acid-mediated oxidative stress and neuroinflammation via targeting GLP-1 signal in MPTP-induced PD mice, suggesting that the compound should be considered as a prebiotic agent to treat PD in the future.
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Advanced Healthcare Materials
·
2023
·
IF 10
Ya Ren,Weiqing Kong,Yihao Liu,Xue Yang,Xiang Xu,Lei Qiang,Xuelian Mi,Changru Zhang,Haoyi Niu,Chengwei Wang,Jinwu Wang
Bone defect repair remains a major clinical challenge that requires the construction of scaffolds that can regulate bone homeostasis. In this study, a photo-cured mesoporous bioactive glass (PMBG) precursor is developed as a tricalcium phosphate (TCP) agglomerant to obtain a double-phase PMBG/TCP scaffold via 3D printing. The scaffold exhibits multi-scale porous structures and large surface areas, making it a suitable carrier for the loading of parathyroid hormone (PTH) (1-34), which is used for the treatment of osteoporosis. In vitro and in vivo results demonstrate that PMBG/TCP scaffolds coordinated with PTH (1-34) can regulate bone homeostasis in a bidirectional manner to facilitate bone formation and inhibit bone resorption. Furthermore, bidirectional regulation of bone homeostasis by PTH (1-34) is achieved by inhibiting fibrogenic activation protein (FAP). Thus, PMBG/TCP scaffolds coordinated with PTH (1-34) are viable materials with considerable potential for application in the field of bone regeneration and provide an excellent solution for the design and development of clinical materials.
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