TALANTA
·
2022
·
IF 6.556
Ming Guan,Xiaojing Kang,Le Wei,Xingjun Hu,Chao Han,Xing Li,Hanwen Liu,Liang Qu,Zhenwen Zhao
Silk, as one of the representative artifacts of China, profoundly affects the communication between eastern and western civilizations, and dyes, as the color support of silks, reflected crucial historical, cultural and technological information. Surface enhanced Raman spectroscopy (SERS) characterized by vibrational information has been extensively employed in dye analysis. However, since natural plants with complex coloring compositions in ancient China were broadly applied in dying textiles, the existing SERS methods often misinterpret results in dye analysis. Besides, semi-quantification of each component was of great difficulty by SERS, limiting the exquisite comparative analyses of different historical samples. For the first time, a dual-mode strategy combining SERS with high mass resolution MALDI FTICR MS was developed in virtue of core-shell silver nanoparticles (AgNPs@PDA), which realized the precise identification and semi-quantification of complex dye mixtures, thus significantly improving the accuracy and applicability of traditional SERS method. Four typical dye components (alizarin, purpurin, berberine and indigo) have been identified and semi-quantified in unearthed dyed silks from Tang Dynasty based on the method. More interestingly, multiple dye components with different contents and their ratio could be precisely determined, which might help in further investigating their dyeing techniques. This dual-mode strategy represents a promising tool for providing solid support for cognition, evaluation and restoration of textile objects in museums and conservation centers.
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ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Guangyao Huang,Hongxin Zhao,Pan Li,Juanjuan Liu,Siyu Chen,Meihong Ge,Miao Qin,Guoliang Zhou,Yongtao Wang,Shaofei Li,Yizhuang Cheng,Qiang Huang,Junfeng Wang,Hongzhi Wang,Liangbao Yang
It is highly challenging to construct the best SERS hotspots for the detection of proteins by surface-enhanced Raman spectroscopy (SERS). Using its own characteristics to construct hotspots can achieve the effect of sensitivity and specificity. In this study, we built a fishing mode device to detect the receptor-binding domain (RBD) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at low concentrations in different detection environments and obtained a sensitive SERS signal response. Based on the spatial resolution of proteins and their protein-specific recognition functions, SERS hotspots were constructed using aptamers and small molecules that can specifically bind to RBD and cooperate with Au nanoparticles (NPs) to detect RBD in the environment using SERS signals of beacon molecules. Therefore, two kinds of AuNPs modified with aptamers and small molecules were used in the fishing mode device, which can specifically recognize and bind RBD to form a stable hotspot to achieve high sensitivity and specificity for RBD detection. The fishing mode device can detect the presence of RBD at concentrations as low as 0.625 ng/mL and can produce a good SERS signal response within 15 min. Meanwhile, we can detect an RBD of 0.625 ng/mL in the mixed solution with various proteins, and the concentration of RBD in the complex environment of urine and blood can be as low as 1.25 ng/mL. This provides a research basis for SERS in practical applications for protein detection work.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 6
·1-10
·
IF 5.479
Cong Lili,Liang Lijia,Cao Fanghao,Sun Dan,Yue Jing,Xu Weiqing,Liang Chongyang,Xu Shuping
A high-throughput, dual-channel single cell analytical method is described for the detection of sialic acid (SA) on single cell based on the use of microfluidic droplets integrated with plasmonic imaging and surface-enhanced Raman spectroscopy (SERS) with the assistance of a multifunctional metal nanoparticle-based probe. The multifunctional plasmonic nanoprobe was prepared by modifying silver nanoparticles (AgNPs) with 4-mercaptophenylboronic acid (MPBA) that both warrants SA recognition and acts as a Raman reporter. This nanoprobe is a high-contrast indicator under bright field imaging due to the strong energy loss feature of AgNPs, and also owns possesses a strong SERS enhancement capability toward MPBA. Cells incubated with the plasmonic nanoprobes were isolated in water-in-oil droplets and then were re-dispersed in a chamber array chip. High-precision profiles of SA on a single cell in one droplet were obtained by the bright field imaging and image processing. The SA expression levels on different cell lines (MCF-7, HepG2, SGC and BNL.CL2) traced by SERS spectroscopy were compared. The statistical data among different cell lines confirm that the SA expression levels on cancer cells are much higher than that on normal cells. Single cell analysis further revealed that the cell-to-cell variations are more obvious in cancer cell lines. This study provides a valuable tool for understanding glycan-related biochemical processes.
A high-throughput, dual-channel microfluidic droplet platform succeeded in distinguishing different cancer cell lines at single living cell level integrated with plasmonic imaging and surface-enhanced Raman spectroscopy with assistance of a multifunctional metal nanoparticle-based probe.
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TALANTA
·
2024
·
IF 5.6
Yue Wang,Bo Lai,Zhi Yu,Zhangrun Xu
Self-driven microfluidic systems have attracted significant attention and demonstrated great potential in the field of point-of-care (POC) testing due to their device simplicity, low power consumption , increased portability, and reduced sample consumption. To develop POC detection chips with diverse characteristics that meet different requirements, there is a strong demand for feasible strategies that enable easy operation and reduce processing time. Here, a one-step processing approach using femtosecond laser direct writing technology was proposed to fabricate a capillary-actuated POC microfluidic chip. The driving force of the chip is highly dependent on its surface wettability , which can be easily adjusted by changing the laser processing parameters . This POC microfluidic chip allowed for the detection of intracellular H 2 O 2 through a catalytic reaction system that incorporated 5-aminosalicylic acid -sensitized colloidal TiO 2 nanoparticles and horse radish peroxidase , with integrating semiconductor-based surface-enhanced Raman scattering (SERS) quantitative technique. The concentration of H 2 O 2 was determined by the SERS signal of the catalytic products in the microfluidic chip, resulting in rapid detection with minimal sample consumption. Our method provides a simple, feasible, and alternative strategy for POC testing of H 2 O 2 , with a linear range of 10 −2 ∼10 −6 M and a limit of detection of 0.55 μM. This approach was successfully applied to rapid detection of intracellular H 2 O 2 in MCF-7 breast cancer cells with high sensitivity and minimal sample consumption. Additionally, this study not only demonstrates the exceptional advantages of femtosecond laser processing technology in fabricating diverse microfluidic chips for various applications, but also presents an efficient POC testing strategy for detecting cell signaling molecules .
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JOURNAL OF RAMAN SPECTROSCOPY
·
2024
·
IF 2.4
Xiujuan Zhang,Yaxin Wang,Yongjun Zhang,Kun Zhang,Ruoning Chu,Fengyi Zhang,Xiaoyu Zhao
Understanding the variation of proton concentration near the surface of the electrochemical interface is of great significance for understanding the mechanism of electrochemical reactions. In this work, 4-Mpy molecules that are protonated and deprotonated depending on the surrounding pH value adsorb on the Au nanoparticle film electrode with high SERS activity, and by virtue of the highly interfacial-sensitive EC-SERS technique, we systematically studied the effects of electrolyte pH value and external voltage on the protonation and deprotonation of 4-Mpy at the interface between Au-NP film electrode and phosphate buffer, to analyze the changes of near-surface proton concentration at the electrochemical interface. It is found that the pH value of the electrolyte plays a decisive role in the protonation process of 4-Mpy at the electrode interface at low reduction voltage (< −0.1 V). In acidic and neutral solution, 4-Mpy exists mainly in protonated form on the electrode surface. However, in alkaline solutions, 4-Mpy exists mainly on the electrode surface in the form of deprotonation. At high reduction voltage (≥ −0.1 V), the protonation and deprotonation of 4-Mpy on the electrode surface are mainly determined by the adsorption structure of 4-Mpy on the electrode surface. At the same time, we conducted a comparative study of 2-Mpy and 4-Mpy molecules and found that the adsorption modes were different depending on the position of the N atom. 2-Mpy is inclined adsorbed on the surface of the Au-NP film electrode, and 4-Mpy is vertically adsorbed on the surface of the Au-NP film electrode.
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OPTICS EXPRESS
·
2019
·Vol. 27
·期 18
·25091-25106
·
IF 3.561
Xu Gao,Hao Zhang,Xiangyu Fan,Chao Zhang,Chao Zhang,Yang Sun,Chundong Liu,Zhen Li,Shouzhen Jiang,Baoyuan Man,Cheng Yang,Cheng Yang
We report a structure to form a hybrid system in which a mesh is sandwiched between Au nanoparticles (AuNPs) and Ag nanoparticles (AgNPs). This self-assembly method uses smaller and denser AgNPs “hot spots” that are spin-coated on a AuNPs@GO mesh nanostructure formed by the reaction of GO@MoS2 and HAuCl4 to form AuNPs@GO mesh@AgNPs SERS substrates. Sub-40-nm mesh and 10-nm gaps ensure the landing sites and spacing of the AgNPs. Consequently, the design integrates the strong plasmonic effects of AgNPs and AuNPs with the biological compatibility of the GO mesh. Crystal violet (CV) as low as 10−15 M can be detected, which confirms the ultrahigh sensitivity of AuNPs@GO mesh@AgNPs. Furthermore, the reproducibility, stability, and finite-difference time-domain (FDTD) simulations confirm the value of this SERS substrate. This material can be used for label-free DNA detection, and the AuNPs@GO mesh@AgNPs substrate facilitated single-molecule DNA detection limits.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Haibin Liu,Tingting Cao,Jing Wang,Yi Yuan,Haijie Li,Kuo He,Hongshuo Chen,Lei Wang
Meat adulteration is a growing concern for consumers and researchers, necessitating the development of a simple and robust method for identifying animal sources in meat products. In this study, a double-tailed recombinase polymerase amplification (RPA) integrated with surface-enhanced Raman scattering (SERS) based two-color lateral flow nucleic acid hybridization strip (LFNAHS) was established for simultaneous detection of pork and horse meat. Magenta and cyan gold nanoparticles functionalized with specific probes and Raman dyestuff were used to color the LFNAHS based on RPA amplification products. The assay successfully detected colored test lines for horse and pork adulteration, with quantitative analysis achieved through Raman intensity measurements. The method showed a high specificity with no cross-reactivity and a detection limit of 0.01 % (wt%). Authenticity testing of 40 donkey products yielded results consistent with PCR-agarose gel electrophoresis. This assay can be extended to identify other meat sources by replacing the specific primer sets. This versatile method can be adapted for identifying other meat sources, making it a valuable tool for accurate, sensitive, and quantitative meat authentication in resource-limited settings.
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TALANTA
·
2025
·
IF 6.1
Wenting Lan,Changgan Lai,Yasheng Dai,Shijiao Fu,Chenjie Gu,Tao Jiang,Yuning Pan
The early and reliable detection of prostate cancer remains a significant challenge in clinical diagnostics. This study presents a quantitative immunoassay for prostate-specific antigen (PSA) using a surface-enhanced Raman scattering (SERS) substrate comprised of polymer-silver nanocubes (Ag NCs) modified with 4-mercaptobenzoic acid (4MBA). We investigated the impact of thiol-containing internal standards (IS) on the quantitative accuracy of SERS, highlighting the superior stability of 4MBA compared to non-thiol molecules like crystal violet (CV). In contrast to CV, 4MBA exhibited significantly greater stability, with only a 9.3% decrease in characteristic peak intensity after prolonged storage. Furthermore, the quantitative detection of PSA was realized using an IS-mediated sandwich immunostructure. Particularly, the coefficient of determination (R 2 ) for the PSA standard calibration curve demonstrated remarkable enhancement from 0.987 to 0.994, highlighting the advantage of thiol-anchored IS correction strategy. Moreover, the limit of detection (LOD) for PSA was refined to 5.6 × 10 -10 mg/mL after a perfect linear fitting was facilitated using 4MBA-based IS correction, which was superior to standard enzyme-linked immunosorbent assay (ELISA) and comparable SERS techniques. In addition, the specificity of the assay was confirmed by the lack of signal for non-target antigens. These findings demonstrate that the strategic selection of robust IS molecules can significantly enhance the quantitative capabilities of SERS technology, paving the way for its application in clinical diagnostics.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Ziyang Bian,Lili Huang,Zetong Li,Jie Wang,Yue Wang,Zhenshan Yang,Hefu Li
The development of versatile surface-enhanced Raman scattering (SERS) platforms facilitates the rapid identification of contaminants in diverse application scenarios. Herein, a dual-functional SERS substrate comprising silver nanoislands-decorated micropillar/wrinkle hierarchical structure (Ag NIs@PWHS) on a polydimethylsiloxane (PDMS) film was fabricated through an amalgamation of fabrication techniques: soft lithography, surface wrinkling, and magnetron sputtering. The PDMS hierarchical architecture was constructed by replicating mastoid structures of lotus leaves through a two-step molding process, followed by the formation of periodic wrinkles via oxygen plasma treatment. This hierarchical design integrating micropillars and wrinkles not only provided a multiscale surface architecture conducive to the formation of three-dimensional (3D) hot spots, but also conferred hydrophobicity and bending insensitivity on the SERS substrate. The optimal Ag NIs@PWHS substrate had an analytical enhancement factor of 1.99 × 10 6 , outstanding signal uniformity (relative standard deviation, RSD = 2.98 %) and reproducibility (RSD = 4.54 %), and robust mechanical stability. Furthermore, the Ag NIs@PWHS substrate enabled the rapid detection of thiram on pear surface and malachite green in lake water, with a detection sensitivity of 10 −7 M for both pesticides. Thus, the developed Ag NIs@PWHS SERS substrate holds significant potential for utilization in realms of food safety assessments and water pollution surveillance.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoyu Yang,Shunbi Xie,Weifen Wu,Jingwen Liu,Manjun Liao,Runzi Zhang,Yao Liu,Mengjun Wang,Yi He
In SERS analysis, SERS substrates often suffer from uneven “hot spot” distribution, poor stability, and susceptibility to oxidation and aggregation, which limit detection precision and accuracy. To overcome these aggregation, this study presents a novel core-shell Au@Ag@hydrogen-bonded organic framework twin cone (Au@Ag@HOF TCs) as efficient SERS substrate. The HOF shell effectively protects Au and Ag from oxidation, thereby enhancing the long-term stability of the SERS substrate. It also prevents signal inhomogeneity caused by noble metal nanoparticle agglomeration and captures target molecules at “hotspots” for maximum Raman enhancement. Leveraging these features, the proposed Au@Ag@HOF TCs enable sensitive detection of bisphenol A, F, and S in food, with detection limits of 1.8033 × 10 −9 M, 4.2170 × 10 −9 M, and 2.0815 × 10 −10 M, respectively. Furthermore, this substrate was successfully applied to analyze real samples, including canned soup, beverages, and milk, demonstrating its potential for the detection of bisphenol compounds in food.
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Nanomaterials
·
2021
·Vol. 11
·期 12
·3460
·
IF 5.076
Guanliang Sun,Ning Li,Dan Wang,Guanchen Xu,Xingshuang Zhang,Hongyu Gong,Dongwei Li,Yong Li,Huaipeng Pang,Meng Gao,Xiu Liang
Rapid and effective detection of pesticide residues from complex surfaces of fruits and vegetables has important significance. Herein, we report a novel three-dimensional (3D) hierarchical porous functional surface-enhanced Raman scattering (SERS) substrate, which is fabricated by successive two-step hydrothermal synthesis strategy of silver nanoparticles (Ag NPs) and cobalt oxide nanowires (Co3O4NWs) on the 3D copper foam framework as Cu@Co3O4@Ag-H. The strategy offers a new avenue for localized plasmonic materials distribution and construction, which exhibits better morphology regulation ability and SERS activity (or hotspots engineering) than physical spurring obtained Cu@Co3O4@Ag-S. The developed Cu@Co3O4@Ag-H possesses large surface area and rich hotspots, which contributes to the excellent SERS performance, including homogeneity (RSD of 7.8%), sensitivity (enhancement factor, EF of 2.24 × 108) and stability. The Cu@Co3O4@Ag-H not only provides plenty of Electromagnetic enhancement (EM) hotspots but also the trace detection capability for droplet rapid sensing within 2 s. Cu@Co3O4@Ag-H substrate is further developed as an effective SERS sensing platform for pesticide residues detection on the surfaces of fruits and vegetables with excellent LOD of 0.1 ppm, which is lower than the most similar reported works. This work offers new potential for bioassay, disease POCT diagnosis, national security, wearable flexible devices, energy storage and other related fields.Keywords:surface-enhanced Raman scattering;Cu@Co3O4@Ag;trace droplet;pesticide residues;3D hierarchical plasmonic nanomaterials;Cu foam framework
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Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 19
·4933-4944
·
IF 4.696
Jing Chen,Gaowu Qin,Qing Chen,Jiangyu Yu,Song Li,Feng Cao,Bo Yang,Yuping Ren
The artistry and the amazingly beautiful hierarchical three-dimensional (3D) patterning of diatoms make them ideal biological scaffolds. Here, fraction-free and ultra-high-purity diatomaceous earth has been successfully prepared by low speed centrifugation in saturated sucrose solution and calcination at 600 °C for 2 h. The prepared citrate-stabilized 18 ± 2.4 and 32 ± 5.4 nm Au nanoparticles were singly, uniformly and densely self-assembled onto amino-functionalized diatomaceous earth templates following their surface morphologies via electrostatic interactions, during which the control experiment and appropriate parameter exploration were performed. The formed 18 ± 2.4 and 32 ± 5.4 nm Au nanoparticle coated diatomaceous earth fine-grained composite materials were pressed into hard, button-like tablets with a fixed diameter of 13 mm and a thickness of 2 mm, and then the two fabricated types of button-like portable tablets were used for surface-enhanced Raman spectroscopy (SERS) detection, and their enhancement factors (EFs), SERS reproducibility and long-term stability were testified using rhodamine 6G (R6G) as the probe molecule. The button-like portable tablet composed of diatomaceous earth-templated 32 ± 5.4 nm Au nanoparticle arrays had a higher EF and was successfully applied to SERS analysis of the trace chemical composition of eccrine sweat in latent fingerprints. It may reveal the medical condition of an individual by analyzing the obtained SERS spectra, suggesting that the SERS-based methodology, due to its high sensitivity, has a potentially wide range of applications in fields that need trace detection, such as medical diagnostics and forensic investigations. Moreover, the button-like, 3D periodically ordered tablet as a portable SERS substrate composed of a synergistic combination of diatomaceous earth with Au nanoparticles in this work is robust, easy to carry around, and applicable to on-site SERS detection.
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