Life-Basel
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2026
·Vol. 16
·期 2
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IF 3.9
Xinyuan Wei,Haoyu Wang,Rui Li,Shengmin Liu,Hongyan Zuo,Qing Hu,Xuliang Zhuang,Zhihui Bai
Petroleum contamination poses a serious threat to human health and ecosystems worldwide, and microbially driven natural attenuation is an effective approach for accelerating hydrocarbon removal. Species of the genusRhodococcusare recognized for their ability to degrade long chain petroleum hydrocarbons. However, their physiological traits and degradation mechanisms under alkaline conditions remain insufficiently understood. In this study, soil samples were collected from the Dagang oilfield in Tianjin, China, andRhodococcussp. A17 was isolated as an active indigenous strain for genomic and physiological characterization under high pH petroleum degradation conditions. The results showed that strain A17 grew optimally at 30 °C, pH 9.0, and 2% salinity. Petroleum hydrocarbon degradation reached 67.8% within 72 h, with a degradation half life of 34.2 h. Genome sequencing identified 18 oxygenase related genes involved in alkane degradation, includingalkB, cytochrome P450 monooxygenases, and the long chain alkane monooxygenaseladA, together with four antibiotic resistance genes. Metabolite analysis suggested that alkane degradation might proceed via terminal and subterminal oxidation pathways. Overall, these findings indicate thatRhodococcussp. A17 exhibits multiple adaptive traits that support its potential application in the bioremediation of petroleum contaminated alkaline environments.
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Bioactive Materials
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2026
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IF 23.6
Wenjie Wang,Ying Ma,Ningyue Deng,Shifan Chen,Ziyue Ling,Yilin Wang,Weifeng Zhao,Changsheng Zhao
Catheter-related infections and obstructions are the primary causes of treatment failure and discontinuation in peritoneal dialysis (PD), constituting a major challenge in the renal replacement therapy for patients with renal failure thereby compromising their treatment. To address the challenge of balancing antimicrobial and anti-fouling properties on medical catheter surfaces, we propose a method that by introducing a slightly positive charge into the anti-fouling matrix to integrate antimicrobial and anti-fouling functions via smart switching behavior. Herein, we developed a polymeric zwitterionic-dominant coating with slightly positive charges (isoelectric point, IEP = 7.69), named PZ-SPC. By forming a hydration layer on the catheter surface, the polymeric zwitterionic hydrogel matrix effectively resisted the non-specific adhesion of proteins, bacteria, and cells on the catheters, thereby preventing bacterial biofilm formation. By adjusting the proportion of organosilicon quaternary ammonium salts (providing long alkyl chains and positive charges) introduced into the matrix, the IEP was precisely tuned to 7.69, which is slightly above the physiological pH (7.4). In a normal peritoneal dialysate environment, the PZ-SPC coating provided a weak net positive charge and hydration layer, thereby exerting a “defensive mode” for effective antifouling function. The adhesion of bovine serum albumin and fibrinogen is reduced by 76.58% and 70.57% respectively. In an acidic biofilm microenvironment, the PZ-SPC coating provided a stronger positive charge to enhance the permeabilization of the bacterial membrane, triggering a “killing mode” for effective antimicrobial action. The bactericidal rate of PD@PZ-SPC against both Gram-negative and Gram-positive bacteria achieves over 99.9% in acidic conditions. We established a peritoneal dialysis catheter-associated peritonitis model in rats to validate the in vivo efficacy of PD@PZ-SPC. The results showed that the PD@PZ-SPC effectively reduced catheter obstruction and alleviated the severity of peritonitis. This coating strategy achieves an optimal balance between “anti-fouling” and “contact sterilization” via intelligent pH-responsive charge switching behavior, providing a practical engineering guideline for developing surfaces to combat diverse contaminants in complex biological environments.
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ACS Omega
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2026
·
IF 4.3
Juefei Wu,Yawei Ge,Qinying Tang,Kang Hu,Yi Wang,Ru Zhang,Yuxiao Mao,Bin Li
Sensitive and biocompatible fluorescent probes capable of simultaneously detecting pH and Fe3+ are crucial for applications in environmental monitoring, biomedical diagnostics, and food safety. In this study, we present an environmentally friendly, one-step hydrothermal synthesis of multifunctional carbon dots (ECDs) utilizing epigallocatechin gallate (EGCG) as a natural carbon precursor. The synthesized ECDs exhibit pronounced pH-dependent fluorescence quenching over a broad pH spectrum (1.3–13.5), characterized by a sigmoidal response (R2 = 0.9918) and remarkable sensitivity within the physiological range (pH 6–8). The mechanism of pH-induced quenching of ECDs has been verified to be the aggregation of ECDs and surface deprotonation under high-pH conditions. Furthermore, the ECDs facilitate highly selective detection of Fe3+ through fluorescence quenching, a process attributed to electron transfer from EGCG-derived phenolic groups. Its fluorescence response shows a linear relationship within the Fe3+ concentration range of 1–125 μM with R2 = 0.995. Additionally, the ECDs demonstrate excellent biocompatibility (cell viability >90%, hemolysis <5%) and exhibit significant antibacterial activity against Escherichia coli and Staphylococcus aureus. This research illustrates a straightforward approach to developing eco-friendly, cost-effective, and multifunctional probes for integrated sensing and antimicrobial applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Yi Chen,Bowen Jia,Junjie Zhou,Guojun Jin,Hou-Yong Yu
Designing oral solid preparations with responsive release mechanisms is crucial for achieving precise therapeutic efficacy in gastrointestinal diseases. In this work, a dual pH/temperature-responsive cellulose nanocrystal (CNCs-D-N) was engineered by grafting a stimuli-sensitive copolymer onto carboxylated cellulose nanocrystal, thereby endowing tailored dual-triggered structural adaptability. The CNCs-D-N exhibited pH-dependent protonation–deprotonation equilibria and thermally induced chain conformational transitions, which cooperatively regulated molecular extension and contraction, enabling rapid and controllable drug release. Tetracycline hydrochloride was subsequently wet-loaded into CNCs-D-N to fabricate stimuli-sensitive tablets (CNC@DNWT), which showed excellent mechanical robustness (hardness: 32.64 N; brittleness: 0.31%), high drug loading capacity (99%), and ultrafast release initiation (7 s). Importantly, CNC@DNWT achieved a cumulative release of 99% under simulated gastric conditions (pH = 3, 37 °C), underscoring synergistic role of dual stimuli in tuning release kinetics. By bridging molecular-level adaptability with macroscopic delivery performance, this study provides a mechanistic strategy for the development of intelligent cellulose-based oral formulations with promising potential for targeted gastric cancer therapy.
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Nature Communications
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2026
·
IF 18.1
Chen Hui,Feng Shuo,Wang Yutong,Zhong Leheng,Liang Wei,Shi Kangning,Li Dedi,Zhu Jiaxiong,Cui Huiling,Tang Zijie,Zhang Haofan,Li Ran,Zhi Chunyi,Lyu Haiming Lv
The electrolytes of aqueous batteries are mostly acidic or alkaline, resulting in inevitable side reactions. More importantly, when batteries are disposed of, strongly acidic and alkaline solutions harm the environment. In this study, we synthesized covalent organic polymers as negative electrode materials for aqueous divalent ion (Mg 2+ and Ca 2+ ) storage, which exhibit favorable performance in electrolytes with a pH of 7.0 (the electrolytes are environmentally benign and can even be “brine” in tofu production). Polymers containing electron-donating linking bonds exhibit fast kinetics and low potential for use in aqueous Mg 2+ /Ca 2+ batteries. In neutral electrolytes (pH = 7.0), Hexaketone-tetraaminodibenzo-p-dioxin covalent organic polymers exhibit 120,000 cycles and deliver specific capacities (up to 112.8 mA h g -1 ). The negative electrode was paired with a positive electrode, creating a full cell that has a voltage interval of 2.2 V and achieves a specific energy of up to 48.3 Wh kg -1 , which is calculated on the basis of the total mass of the negative electrode, positive electrode, and electrolyte at the full cell level, together with long-term cycling stability over 120,000 cycles at a specific current of 20 A g -1 . The full cells are environmentally benign and nontoxic and can be directly discarded to environments according to various standards (GB 18599-2020, ISO 14001, Resource Conservation and Recovery Act RCRA, US).
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LANGMUIR
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2026
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IF 4.4
Chunfeng Zhang,Yunyou Lv,Meiqi Guan,Yu Lu,Elin Li,Peiyao Wu,Weijie Li,Fang Yang,Jie Liu
The development of intelligent nanocarriers capable of overcoming the intrinsic limitations of conventional pesticides, including poor foliar adhesion, photodegradation, and nonspecific release, remains a major challenge in agrochemical science. Herein, we report a multifunctional core–shell nanocarrier (Pro@ZnO@PDA, denoted as PZP NPs) constructed via interfacial engineering, in which prochloraz-loaded ZnO nanoparticles (ZnO NPs) are encapsulated within a polydopamine (PDA) shell. The rough surface of the ZnO core enables a high pesticide loading capacity of 12%, while the PDA shell markedly enhances leaf adhesion, reducing the contact angle on plant leaves by 25.4%, thereby improving foliar retention. Benefiting from acid-sensitive interfacial dissociation between the PDA shell and ZnO core, the nanocarrier exhibits pH-responsive release behavior, achieving a targeted prochloraz release of 76% under acidic conditions (pH 5.4). In addition, the ZnO core effectively shields the active ingredient from ultraviolet irradiation, resulting in a 23.3-fold enhancement in photostability, whereas the PDA shell provides efficient photothermal conversion, inducing an elevation of temperature up to 38.8 °C under light exposure. The integration of controlled chemical release and photothermal effects gives rise to a synergistic antifungal mechanism, maintaining an inhibition rate exceeding 60% after 7 days of irradiation. Notably, PZP NPs exhibit bidirectional translocation within plants, addressing the limited systemic transport of conventional fungicides. This work demonstrates an interfacial-engineered, stimulus-responsive nanoplatform that offers a promising strategy for intelligent and efficient pesticide delivery.
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
·
IF 15
Yunfan Li,Kaiwen Bao,Renzheng Huan,Tian Wang,Ya Wang,Shuai Wu,Xin Chen,Jiashang Huang,Li Zhu,Jianshu Li,Haifeng Yang,Wei Wu
Glioblastoma (GBM) is one of the most aggressive malignancies of the central nervous system. Gemcitabine (GEM), a pyrimidine analogue with broad-spectrum anticancer activity, can activate the cGAS-STING pathway and alleviate the immunosuppressive microenvironment of GBM. However, its clinical application is hampered by the formidable challenge of crossing the blood-brain barrier (BBB) and accumulating at the tumor lesion. Herein, a dual-responsive biomimetic nanoprodrug (RMM@GEM NPs) was exploited to enhance the efficient BBB penetration and target cargo delivery by functionalization of glioblastoma cell membranes (MM) camouflaging and further targeting peptide RAP modification. After its selective accumulation at glioma lesion, RMM@GEM NPs accelerates GEM release under the tumor pathological stimuli of reactive oxygen species (ROS) and acidic microenvironment to robustly activate the STING signaling cascades (increased p-STING, p-TBK1, p-IRF3, and p-NF-κB). Simultaneously, cyclodextrin-mediated cholesterol depletion further suppresses PD-L1 expression and alleviates T-cell exhaustion. These findings highlight RMM@GEM NPs as a promising strategy to enhance immune responses in “cold” tumor, providing a potential candidate for efficient and safe immunotherapy in GBM.
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Journal of Materials Chemistry A
·
2026
·
IF 9.2
Jiao Yang,Lishan Peng,Youpeng Cao,Lun Li,Zhichao Yu,Chengcheng Zhong,Wendi Zhang,Weng Fai Ip,Hui Pan
The precise construction of highly dispersed monometallic composites with abundant grain boundaries, beyond conventional gradient or core–shell structures, remains a key challenge. Herein, we report a chlorine-mediated strategy to achieve monometallic Ru–RuO2 featuring a high density of well-defined heterointerfaces. By leveraging the distinct binding energies of Ru–O and Ru–Cl, a controlled in situ reduction process selectively converts Cl-occupied regions in a RuOCl precursor into metallic Ru domains, yielding an intimately interwoven Ru/RuO2 heterostructure. This unique architecture provides a high density of interfacial active sites, leading to exceptional pH-universal hydrogen evolution reaction (HER) performance. The optimized Ru/RuO2 catalyst achieves remarkably low overpotentials of 22, 54, and 26 mV at 10 mA cm−2 in 1 M KOH, 1 M phosphate buffer solution (PBS), and 0.5 M H2SO4, respectively, surpassing commercial Pt/C. Density-functional-theory (DFT) calculations illustrated that the remarkable HER activity stems from synergistic metal–oxide interactions: RuO2 promotes efficient water dissociation, while adjacent metallic Ru sites optimize hydrogen adsorption, collectively accelerating the HER kinetics. This work provides a general design strategy for constructing high-performance heterointerface-enriched electrocatalysts.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Jiahui Gu,Xiliang Zhang,Zhengyin Ding,Kaiyue Wang,Yunfeng Xu,Shuang Liu,Haiqin Gu
The super-hydrophilic TiO₂ pH electrode was modified by oxygen-enriched annealing. • This post-treatment offered tunable production cost suitable for controlled-environment agriculture. • The underlying influence mechanism of modification has been elucidated. • Optimized electrode improved surface hardness and hydrophilicity with stable electrochemical response. • Durability assessment showed lower pH measurement error in unsaturated cultivation substrates. With the escalating demand for rapid and accurate pH detection in controlled-environment agriculture, super-hydrophilic TiO₂ pH electrodes fabricated via chemical etching have emerged as promising candidates for real-time sensing in cultivation substrates, offering tunable production cost and reliable performance. However, their practical deployment is often constrained by limited mechanical durability and unstable interfacial wetting in heterogeneous substrates. In this work, an oxygen-enriched annealing strategy is employed to deliberately tailor the surface structure of TiO₂ electrodes, aiming to enhance interfacial wettability and mechanical robustness rather than solely maximizing intrinsic sensitivity in buffer solutions. This post-treatment significantly modulates the surface morphology, roughness, and composition of the electrodes, elucidating the effects of annealing temperature and duration on their mechanical and wetting properties. The electrode annealed at 350 °C for 30 min (T350-30) developed a uniform interconnected porous microstructure, improving hardness to 208.7 HV0.05 and reducing contact angle to 8.57°. The enlarged specific surface area and abundant amorphous TiO₂ preserved stable pH response, delivering 50.9 mV/pH sensitivity, ≤10 s response time, as well as minimal drift and hysteresis. Durability tests in 66.7% cultivation substrates showed measurement error increased only from 0.07 to 0.14 pH units after 30 insertion–removal cycles, compared with 0.08 to 0.28 pH units for the unannealed electrode. In addition, the annealed electrode exhibited improved stability under sequential exposure to aqueous solutions and ambient air, supporting its stability for practical open-environment. Download: Download high-res image (158KB) Download: Download full-size image
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International Journal of Nanomedicine
·
2026
·
IF 8.7
Feixiao Ma,Anqi Ji,Beng Ma,Pingjuan Yang,Jiayi Li,Shuxin Liao,Haonan Yao,Xuanhu Xie,Zhiwei Wang,Linlin Shi,Shegan Gao,Xin Hou
Purpose Esophageal cancer (EC) ranks among the most prevalent and lethal malignancies worldwide, and conventional therapeutic modalities, including surgery, radiotherapy, and chemotherapy, are constrained by inherent limitations. Recent advances in nanomedicine have opened new avenues for EC treatment. To achieve efficient and precise tumor eradication, this study developed a pH-responsive composite nanoplatform featuring a hollow architecture to enable high-efficiency loading of photosensitizers and realize multimodal synergistic therapy against EC through the integration of photothermal therapy (PTT), photodynamic therapy (PDT), and chemodynamic therapy (CDT).Methods A pH-responsive Cu2-xSe/PDA/IR820 composite nanoplatform was constructed by coating hollow Cu2-xSe nanoparticles with polydopamine PDA and loading the near-infrared photosensitizer IR820. Morphology, crystalline phase, elemental composition, and surface chemistry were characterized by TEM, XRD, XPS, and FT-IR. Photothermal conversion efficiency was quantified under 808 nm laser irradiation. IR820 release kinetics were monitored under different pH conditions of 5.0, 6.0, and 7.0 to assess pH-responsive behavior. In vitro studies on KYSE-150 EC cells included viability by CCK-8 assay, apoptosis by flow cytometry, ROS generation by DCFH-DA probe, mitochondrial membrane potential by JC-1 staining, and cellular uptake analysis. In vivo antitumor efficacy and biosafety were evaluated in an AKR tumor-bearing C57BL/6J mouse model via intratumoral injection and 808 nm laser irradiation, followed by tumor volume measurement, histopathological analysis with H&E, Ki-67, and TUNEL staining, and systemic toxicity assessment.Results The hollow Cu2-xSe/PDA/IR820 nanoparticles exhibited a uniform size of approximately 164 nm, excellent colloidal stability, and a high photothermal conversion efficiency of 44.56% under 808 nm laser irradiation. IR820 release displayed pronounced pH sensitivity, with a cumulative release of 23.07% at pH 5.0 within 30 minutes. In vitro, the nanoplatform combined with laser irradiation reduced KYSE-150 cell viability to 1.2% at 100 μg/mL, with an IC50 value of 18.74 μg/mL, induced 28.57% apoptosis, elevated intracellular ROS levels, decreased mitochondrial membrane potential, and depleted glutathione to 335 μmol/gprot. In vivo fluorescence imaging confirmed effective tumor accumulation via the EPR effect, with peak signal at 24 hours post-injection. Combined PTT/PDT/CDT treatment significantly suppressed tumor growth, reducing final tumor volume to 17.8% of the control group, and induced extensive apoptosis and necrosis in tumor tissues. Systemic biosafety evaluations revealed no significant hematological or histopathological abnormalities.Conclusion Hollow Cu2-xSe/PDA/IR820 composite nanoparticles loaded with IR820 were successfully fabricated and demonstrated remarkable advantages in multimodal combination therapy against EC.
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Journal of Biological Engineering
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2026
·Vol. 20
·期 1
·94
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IF 6.3
Ye Gaobing,Liu Jing,Jin Yu,Hu Renren,Tian Tianli,Chen Peilei
Tumor hypoxia limits type-II photodynamic therapy (PDT) by restricting oxygen-dependent singlet oxygen production during irradiation. Here, we developed a pH-responsive ZnO 2 @ZIF-8/TCPP nanoplatform (PZZT) that integrates an acid-activated oxygen-generating core (ZnO 2 ), a ZIF-8 gatekeeper shell, and the photosensitizer TCPP, aiming to enhance PDT under hypoxic conditions. Under mildly acidic conditions, PZZT showed improved oxygen generation and enhanced DPBF consumption upon 660 nm irradiation, indicating elevated ¹O₂ production. In 4T1 cells, PZZT + laser induced stronger intracellular ROS signals and greater phototoxicity than free TCPP or control formulations. In 4T1 tumor-bearing mice, PZZT + laser achieved the most significant tumor growth inhibition without obvious systemic toxicity. Overall, PZZT provides a practical pH-activated self-oxygen-supplying strategy to improve PDT efficacy.
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OPTICS AND LASER TECHNOLOGY
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2026
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IF 5
Pengxiao Guo,Kanglei Wang,Huijing Du,Lei Zhang,Jianshe Li,Li Huo,Shuguang Li
In order to meet the requirements of real-time control of multiple parameters in biopharmaceutical processes, this paper proposes an SPR fiber optic sensor with spectral selectivity for simultaneous measurement of glucose concentration, pH value, and temperature. The sensor is a triple cascade structure, with an Ag film used to measure glucose concentration, PAA/CS self-assembly is performed on the Ag/TiO 2 surface for measuring pH, and Ag/MoS 2 /PDMS is used for measuring temperature. The innovation of this paper lies in utilizing the different optical properties of TiO 2 and MoS 2 and the response differences of functional films to different measured parameters, to endow the three-sensor band spectral selectivity, and achieve distinguishable and non-interfering synchronous responses of three parameters within the full spectrum (400–1700 nm). The experimental results showed that the sensitivity of sugar content (0–35 %) and temperature (−10–100 °C) were 5.29 nm/% and 6.8 nm/°C, respectively. At the same time, pH could be accurately measured in the range of 3.22–10.12, with detection limits of 0.151 %, 0.117 °C, and 0.043 pH, respectively, demonstrating good stability and covering the key range of typical fermentation processes. The functional film design and spectral selection mechanism proposed in this study have great potential for application in precisely grasping key parameters in biological fermentation and pharmaceuticals, and provide a new paradigm for the structural integration of multi-parameter sensors.
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