TOXICOLOGY AND APPLIED PHARMACOLOGY
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2022
·
IF 4.46
Yuan-Lin Zhang,Hong-Xia Yuan,Jian Sun,Xiu-E Feng,Jin-Hong Ren,Yuan-Biao Qiao,Qing-Shan Li
Directly targeting caveolar caveolin-1 is a potential mechanism to regulate endothelial permeability, especially during oxidative stress , but little evidence on the topic limits therapeutics discoveries. In this study, we investigated the pharmacological effect of an antioxidant LM49 (5,2′-dibromo-2,4′,5′-trihydroxydiphenylmethanoe) and its five diphenylmethanone derivatives on endothelial permeability and establish two distinct mechanisms of action. Multiplex molecular assays with theoretical modeling indicate that diphenylmethanone molecules, including LM49, directly bind the caveolin-1 steric pocket of ASN53/ARG54, ILE49/ASP50, ILE18, LEU59, ASN60, GLU48 and ARG19 residues. They also indicated dynamic binding-affinity for diphenylmethanone derivatives. First, this molecular interaction at caveolin-1 pocket inhibits its phosphorylation at TYR14 residue in H 2 O 2 -injured endothelial cell. A positive correlation was established between diphenylmethanone derivative binding-affinity and caveolin-1 phosphorylation inhibition. Inhibition of caveolin-1 phosphorylation, however, was independent of the LM49-mediated variation of protein tyrosine kinase activity, suggesting a direct blockage of adenosine triphosphate substrate diffusion into cavelion-1 structure. Second, LM49 increases the expression of cellular adhesive and tight junction proteins , VE-cadherin and occludin , in H 2 O 2 -injured cell, in a dose dependent manner. A leakage assay of fluorescein isothiocyanate-labeled dextran 40 across cell monolayer suggested improvement in endothelial barrier integrity with diphenylmethanone treatments. Our results demonstrate a direct targeting effect of caveolin-1 on endothelial permeability, and should guide the diphenylmethanone therapy against oxidative stress-induced junction dysfunction, especially at caveolar membrane invagination.
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ACS Catalysis
·
2021
·
IF 13.084
Yiwen Yang,Haifeng Qi,Huixiang Li,Zhanwei Xu,Xiumei Liu,Shuyin Yu,Zongchao Conrad Zhang
Efficient hydrolysis of β-1,4-glycosidic bonds of cellulose is a pivotal step in biorefineries of cellulosic biomass. In this work, we discovered that paired PdCl2–CuCl exhibited excellent synergetic catalytic activity over the single PdCl2 or CuCl in the hydrolysis of β-1,4-glycosidic bonds in 1-butyl-3-methylimidazolium chloride ([BMim]Cl) and further investigated the catalytic species in this system. The catalytic activity increased and reached a plateau at a CuCl/PdCl2 ratio of 1 with increasing molar ratio of CuCl/PdCl2 while keeping the Pd concentration constant. A combination of experimental evidence based on in situ far-infrared absorption, X-ray absorption, X-ray photoelectron, and electron paramagnetic resonance spectroscopies and DFT calculations demonstrate the coupling reaction between [PdIICl4]2– and [CuICl3]2– via the Cl bridge to form the heterometallic complex anion [Cl2PdII–Cl–CuICl2]2–. The heterometallic species is stable during the reaction and maintains its catalytic performance in the presence of various oxygenate additives. Using paired PdCl2–CuCl as a catalyst, the activation energy of hydrolytic cleavage of β-1,4-glycosidic bonds of cellobiose is determined as low as 95.8 kJ/mol. PdCl2–CuCl/[BMim]Cl combined with CrCl3 can be reused five times in cellobiose conversion to 5-hydroxymethylfurfural (5-HMF) without an apparent decrease of catalytic activity. The performance of this catalytic system is further verified for efficient cellulose conversion. More than 60% product yield is obtained at 100 °C from the cellulose, affording monosaccharides for efficient production of high value-added chemicals, such as 5-HMF, when CrCl3 is present to catalyze the conversion of glucose to 5-HMF.
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Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2021
·
IF 3.057
Qinqin Xu,Zikai Liu,Chen Yan,Runhua Lu,Wenfeng Zhou
In this study, a novel sorbent, 1-octyl-3-methylimidazolium hexafluorophosphate functionalised magnetic poly β-cyclodextrin, was successfully synthesised and applied to magnetic solid-phase extraction for the determination of pyrethroids in tea infusions. The sorbent was characterised by transmission electron microscopy, energy-dispersive spectroscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, vibrating sample magnetometer and Brunauer–Emmett–Teller measurement. All factors affecting extraction efficiency, such as sorbent amount, extraction time, ionic strength and desorption conditions, were optimised individually. Under the chosen conditions, wide linearity (2.5–500 μg L−1) with determination coefficients ranging from 0.9995 to 0.9999, low limits of detection of 0.32–0.54 μg L−1 and good precision (intra-day: 2.6–7.0%; inter-day: 3.5–7.6%) were achieved for four pyrethroids in tea infusions. The relative recoveries of target analytes in real tea infusion samples were from 70% to 101% with relative standard deviations lower than 9.1%. We conclude that the proposed method is promising in the detection of pyrethroids in tea infusions.
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NEUROCHEMICAL RESEARCH
·
2021
·Vol. 47
·期 2
·264-278
·
IF 3.996
Gao Tianyu,Gao Ce,Liu Zhidong,Wang Yun,Jia Xiaoxia,Tian Hao,Lu Qian,Guo Lin
Further understanding the mechanism for microglia activation is necessary for developing novel anti-inflammatory strategies. Our previous study found that the activation of sigma-1 receptor can effectively inhibit the neuroinflammation, independent of the canonical mechanisms, such as NF-κB, JNK and ERK inflammatory pathways. Thus, it is reasonable that an un-identified, non-canonical pathway contributes to the activation of microglia. In the present study, we found that a sigma-1 receptor agonist of 2-morpholin-4-ylethyl 1-phenylcyclohexane-1-carboxylate (PRE-084) suppressed lipopolysaccharide (LPS) elevated nitric oxide (NO) content in BV-2 microglia culture supernatant and LPS-raised mRNA levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) in BV-2 microglia. Moreover, PRE-084 alleviated LPS-increased Ser 9 de-phosphorylation of glycogen synthase kinase-3 beta (GSK-3β), LPS-elevated catalytic activity of calcineurin, and LPS-raised percent and frequency of Ca 2+ oscillatory BV-2 cells. We further found that the inhibitory effect of PRE-084 was reversed by a calcineurin activator of chlorogenic acid and a GSK-3β activator of pyrvinium. Moreover, an IP 3 receptor inhibitor of 2-aminoethoxydiphenyl borate mimicked the anti-inflammatory activity of PRE-084. Thus, we identified a noncanonical pro-neuroinflammary pathway of Ca 2+ oscillation/Calcineurin/GSK-3β and the inhibition of this pathway is necessary for the anti-inflammatory activity of sigma-1 receptor activation.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-9
·
IF 5.833
Tang Zhuoyun,Wei Zeliang,Huang Ke,Wei Yinhao,Li Dongdong,Yan Shixin,Huang Jin,Geng Jia,Tao Chuanmin,Chen Piaopiao,Ying Binwu
Human immunodeficiency virus (HIV) infection inflicts significant economic and social burdens on many countries worldwide. Given the substantial morbidity and mortality from HIV infection, there is an urgent need for accurate and early detection of the virus. In this study, immunofluorescence and visual techniques are described that detect the HIV-1 p24 antigen, which relied on selective recognition of Ag + /Ag nanoparticles (Ag NPs) and Cu 2+ /Cu + using cadmium telluride quantum dots (CdTe QDs). After the sandwich immunoreactions were accomplished, the alkaline phosphatase (ALP) hydrolyzed L-ascorbic acid 2-phosphate (AAP) to form ascorbic acid (AA) that further reduces Ag + and Cu 2+ to Ag NPs and Cu + , respectively. This method was highly sensitive and selective and could detect as low as 1 pg/mL of p24 antigen by naked eyes and had a good linearity in the concentration range 1–100 pg/mL. When using Ag + and Cu 2+ as media, the limit of detection (LOD) of the new method was 0.3 pg/mL and 0.2 pg/mL, respectively. Compared with clinical electrochemiluminescence immunoassay (ECLIA) results and clinical data, this method demonstrated good consistency for the quantification of HIV-1 p24 antigen in 34 clinical serum samples. In addition, this method could accurately distinguish HIV from other viruses and infections such as hepatitis B virus , systemic lupus erythematosus , hepatitis C virus , Epstein-Barr virus , cytomegalovirus , lipemia , and hemolysis . Therefore, our dual-mode analysis method may provide additional solutions to identify clinical HIV infection. Graphical abstract An immunofluorescence and visualization dual-mode strategy for the detection of p24 antigen was constructed based on immune recognition reaction and a phenomenon that cadmium telluride quantum dots (CdTe QDs) can selectively recognize Ag + /Ag nanoparticles (Ag NPs) and Cu 2+ /Cu + .
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JOURNAL OF HAZARDOUS MATERIALS
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2021
·
IF 10.588
Mingzhu Liu,Nannan Wu,Bingru Tian,Dongmei Zhou,Chao Yan,Zongli Huo,Ruijuan Qu
The oxidation of Benzophenone-1 (BP-1) by ferrate (Fe(VI)) was systemically investigated in this study. Neutral pH and high oxidant dose were favorable for the reaction, and the second order rate constant was 1.03 × 10 3 M −1 ·s −1 at pH = 7.0 and [Fe(VI)] 0 :[BP-1] 0 = 10:1. The removal efficiency of BP-1 was enhanced by cations (K + , Ca 2+ , Mg 2+ , Cu 2+ , and Fe 3+ ), while inhibited by high concentrations of anions (Cl - and HCO 3 - ) and low concentrations of humic acid . Moreover, intermediates were identified by LC-MS, and five dominating reaction pathways were predicted, involving single hydroxylation, dioxygen transfer, bond breaking, polymerization and carboxylation . Theoretical calculations showed the dioxygen transfer could occur by Fe(VI) attacking the C C double-bond in benzene ring of BP-1 to form a five-membered ring intermediate, which was hydrolyzed twice followed by H-abstraction to generate the dihydroxy-added product directly from the parent compound. Dissolved CO 2 or HCO 3 - might be fixed to produce carboxylated products, and Cl - led to the formation of two chlorinated products. In addition, the toxicity assessments showed the reaction reduced the environmental risk of BP-1. This work illustrates Fe(VI) could remove BP-1 in water environments efficiently, and the newly proposed dioxygen transfer mechanism herein may contribute to the development of Fe(VI) chemistry.
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CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Pan Liu,Qin-Hao Zhang,Jian-Qing Zhang,Ji-Ming Hu,Fa-He Cao
A method to quickly prepare highly oriented silicalite-1 zeolite films on different substrates at lower temperature was provided in this work. Compared with the traditional hydrothermal synthesis, rubbing a layer of seed pastes on the pretreated substrate surface in advance and using (NH 4 ) 2 SiF 6 as silicon source for subsequent secondary growth process can effectively reduce the synthesis temperature and shorten the contact time between the substrate and the strong alkaline synthesis solution. The thickness of the zeolite films prepared under three optimal conditions is all around 1 μm and the best contact angle value of 142.5° was achieved for the film prepared at 373 K for 4 h, while this values of the film prepared at 363 K for 6 h and 353 K for 10 h were 97.8° and 41.7°, respectively. The results of SECM showed that the detected localized current value of the film prepared at 353 K for 10 h was similar to that on bare steel after immersion in 0.62 M NaCl solution for 24 h (around 0.4 nA). At the same time, the current value measured under the other two preparation conditions was much higher, suggesting that the corrosion resistance of the film prepared under 353 K was unsatisfactory. In addition, EIS results demonstrated that the corrosion resistance of the films prepared at 363 K for 6 h also decreased significantly after 72 h immersion, while the films prepared at 373 K for 4 h still maintained excellent corrosion resistance and no obvious corrosion products were detected even immersion time up to 720 h. This work provides a new strategy for the application of zeolite in the field of material protection.
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ACTA BIOCHIMICA POLONICA
·
2021
·Vol. 68
·期 4
·765-774
·
IF 2.149
Shaoyi Li,Yong Li,Bibo Tan,Zhaojie An
Krüppel-like factor 1 (KLF1) is a transcription factor that exhibits promoting effect in cervical cancer, but its correlation with gastric cancer (GC) has not been reported yet. In this study, we explored the role and potential mechanism of KLF1 in GC progression by using a series of experimental methods including RT-qPCR, Western blot, CCK-8 assay, EdU staining, and cell cycle analysis. KLF1 was found to be elevated in GC tissues (n=415) compared with the normal tissues by applying UALCAN to analyze datasets from The Cancer Genome Atlas (TCGA). The upregulation of KLF1 was also validated in GC cell lines. Functional studies proved that RNA interference-mediated silencing of KLF1 inhibited GC cell growth, as evidenced by the decreased cell viability, DNA synthesis, and arrested cell cycle in G1 phase. Moreover, KLF1 knockdown exerted the inhibitory effects on cell migration and invasion as well as the epithelial-mesenchymal transition (EMT) in GC cells. Conversely, overexpression of KLF1 had the opposite effects on GC progression. Furthermore, we proved that the activation of Wnt/β-catenin pathway was markedly inhibited by KLF1 knockdown and promoted by KLF1 overexpression. The blockade of Wnt/β-catenin pathway rescued the effects of KLF1 overexpression. These results suggested that KLF1 promoted the growth, migration, invasion, and EMT process in GC cells, and this promotion was achieved by activating the Wnt/β-catenin pathway. This work will be helpful for searching the potential therapeutic targets for treatment of GC.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Renxin Wang,Yuanzuo Zou,Jingzhou Guo,Yuan Pu,Dan Wang
1,3,5-Tris(1-phenyl-1H-benzimidazol-2-yl)benzene (TPBi) is normally used as functional materials in organic light-emitting diodes (OLEDs) and the information about the solubilities of TPBi in organic solvents is crucial for the preparation of inks toward inkjet printing technology, which is considered to be a next-generation manufacturing method for OLED production. The solubilities of TPBi in 12 kinds of organic solvents, including n-hexane, cyclohexane, methanol, ethanol, isopropanol, n-butanol, acetonitrile, ethyl acetate, dimethyl sulfoxide, tetrahydrofuran, chlorobenzene, and trichloromethane, at the temperature range from 283.15 to 323.15 K and atmospheric pressure were determined based on the isothermal saturation method. The obtained solubility data were correlated by the modified Apelblat equation and the correlated results exhibited good agreement with the experimental data. The nanonization of TPBi was conducted via high gravity-assisted solvent/antisolvent precipitation processing, which showed a beneficial effect for film formation.
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MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Yawen Zhang,Xia Wang,Xiang Fang,Xianxian Yuan,Huaixia Yang,Jinming Kong
As importance of early diagnosis, prognosis, and clinical monitoring in improving survival rate of lung cancer is increasing, the advance of effective diagnostic method for tumor marker is crucial. Herein, linear poly( ε -caprolactone)- b -poly(ethylene oxide) (PCL-PEO) copolymer with hydrophilic property is applied to detect CYFRA 21-1 in label-free electrochemical immunoassay. 4-(2-trimethylsilylethynyl)benzoic acid (TEB) as “bridge” bond first connects initial antibody to the surface of gold electrode. Then one side of CYFRA 21-1 antigen can recognize with initial antibody of electrode interface, and the other side hybridize specifically secondary antibody grafted PCL-PEO copolymer. So far we have constructed a complete immunoassay for CYFRA 21-1 detection using electrochemical impedance spectroscopy. The sensitivity and selectivity of analytical method depend on “Au–C” bonding, specific recognition of “antibody-antigen–antibody sandwich”, and PCL-PEO copolymer. Under optimal conditions, the analytical method for quantitative detection of CYFRA 21-1 manifests a broad linear range (1 pg/mL–10 ng/mL) and a low detection limit (0.125 pg/mL, about 3.47 × 10 -15 M). Furthermore, according to the analysis results in clinical samples, the constructed strategy also shows satisfactory sensitivity and anti-interference. It indicates that the immunoassay has broad promising applications for expecting to be applied in practical clinical prevention and monitoring.
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Journal of Analysis and Testing
·
2021
·Vol. 5
·期 4
·387-395
·
IF 0
Zhou Yufeng,Duan Liguang,Huang Yixin,Liu Yaqi,Wang Lijuan,Qiao Xiaoqiang
The amino acids post-modified ionic liquids-based hybrid monolithic columns were successfully developed. For the first step, using 1-vinyl-3-dodecylimidazole bromide (VDI·Br) and polyhedral oligomeric silsesquioxane methacryl substituted (POSS-MA) as monomer and crosslinking agent, the monolithic POSS-VDI matrix material was polymerized in 100 μm capillary. The prepared POSS-VDI column is of ion-exchange/reverse-phase mixed-mode retention characteristics. The column efficiency reached 137,000 N/m for thioureas. The POSS-VDI column has also been successfully applied to recognize and analyze the effective components of Traditional Chinese Medicines Fangji and the Root of Kudzu Vine. Furthermore, based on its good chromatographic performances, in the second step, amino acids l -Proline ( l -Pro), Glycine (Gly) or DL-Phenylalanine (DL-Phe) were further exploited as the anion exchangers to post-modify the POSS-VDI column. The new POSS-VDI-Pro, POSS-VDI-Gly and POSS-VDI-Phe columns exhibited different separation selectivity for the complex egg white protein sample. The present work provides a good route to fabricate versatile amino acids and ionic liquids modified monolithic columns with good separation selectivity.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Li Shen,Fan Liu,Yao Shen,Cheng Sun,Yuchi Zhang,Qiaoli Wang,Sujing Li,Wei Li
Biphasic solvents have been attracting increasing attention in CO 2 capture recently due to its energy-saving potential. However, most current biphasic solvents still face the problem of limited desorption performance. To solve the problem, a novel biphasic solvent of 1-(2-aminoethyl)piperazine (AEP)/1-propanol/H 2 O solution was proposed. The absorption capacity was 1.26 mol CO 2 ·mol −1 amine, which was 2.3 times that of MEA. The regeneration efficiency was 74% and the cyclic capacity after 5 absorption-desorption experiments was 0.90 mol CO 2 ·mol −1 amine, which was 2.7 times that of MEA. The absorption-desorption performance of AEP/1-propanol/H 2 O solution was much better compared with most current biphasic solvents. Meanwhile, AEP/1-propanol/H 2 O solution had a low regeneration heat of 2.74 GJ·t −1 -CO 2 , which was 27.9% lower than that of MEA. Moreover, 13 C NMR analysis was used to investigate the absorption-desorption mechanism and species distribution. The multiple amino groups in AEP could react with CO 2 to produce carbamate and carbonate. The generated absorption products were mainly concentrated in the CO 2 -rich phase, while the CO 2 -lean phase contained much 1-propanol. Most of the absorption products were decomposed during regeneration, so the high desorption performance was achieved. Overall, AEP/1-propanol/H 2 O solution is a promising energy-saving candidate for industrial CO 2 capture due to its competitive absorption-desorption performance and low regeneration energy.
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