CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Juntao Tang,Mengying Fu,Yidan Mao,Shuyi Yang,Wei Lin,Yan Yu,Shuang Song
Photocatalysis is regarded as a promising in situ air purification technology for the removal of volatile organic compounds. However, the deactivation of photocatalysts remains a serious issue that needs to be addressed. Herein, we report a novel dual-functional photocatalyst (TiO 2 @HYZ) with spatially separated catalytic sites and adsorption sites, which has been obtained by combining hydrophilic {0 0 1} TiO 2 nanosheets with hydrophobic NaY zeolite. TiO 2 @HYZ showed excellent photocatalytic activity for the degradation of gaseous toluene under UV irradiation in humid air. The optimized TiO 2 @HYZ-2 (containing 45.7 wt% TiO 2 ) achieved 96.6% toluene removal and 87.4% mineralization within 120 min. The specific reaction rate constant of TiO 2 @HYZ-2, pristine TiO 2 and P25 normalized by the unit amount of TiO 2 during the photocatalytic degradation process were calculated to be 0.481, 0.230 and 0.250 min −1 g −1 , respectively. Moreover, TiO 2 @HYZ-2 showed superior durability, maintaining stable performance over repeated toluene degradation processes, even at high toluene concentration, whereas pristine TiO 2 incurred severe deactivation with increasing number of photocatalysis cycles. The significantly enhanced activity and anti-deactivation property was attributed to a synergistic effect between HYZ and TiO 2 in the catalyst structure, which strengthened the adsorption of toluene on HYZ and facilitated effective separation of photoexcited charge carriers on TiO 2 , thus avoiding the deposition of degradation intermediates on the active sites and simultaneously improving mineralization. This work provided a new perspective for the design of efficient and deactivation-resistant photocatalysts for air pollution remediation.
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Nanoscale
·
2022
·Vol. 14
·期 39
·14585-14593
·
IF 8.307
Zhiyong He,Yuexu Xiong,Wenlin Feng
Rhenium (Re)-based transition metal dichalcogenides (TMDs) have excellent in-plane anisotropic optical and electrical properties. However, their distorted octahedral (1T′) structure and weak interlayer coupling easily lead to anisotropic and out-of-plane growth, which makes it particularly difficult to prepare large-area Re-based TMD continuous porous films on SiO2/Si substrates. In this work, ReS2 films are synthesized on SiO2/Si substrates by using tellurium (Te) powder-assisted chemical vapor deposition, and then the films are selenized to synthesize a series of continuous large-area ReS2(1−x)Se2x (x = 0, 0.34, 0.56, 0.84, and 0.91) nanosheet-formed porous films. Furthermore, prototype ReS2(1−x)Se2x photodetectors with different Se compositions are fabricated. The surface morphology, high quality crystallization and compositions are confirmed by various characterization techniques. The ReS2(1−x)Se2x photodetectors based on these films show excellent ultraviolet-visible (UV-vis) spectral responses and self-powered characteristics. The response time is faster, and the photocurrent increases with the Se composition. Due to the Schottky barrier generated by the Ag–ReS2(1−x)Se2x interface, the device without bias voltage has a superior responsivity (121.9 mA W−1), high detectivity (5.27 × 1012 Jones), good on/off ratio (1.2 × 103) and fast response time (rising/decay times, 30/60 ms) under 365 nm light irradiation. This simple and controllable method opens up a new way to produce high-quality vertically oriented ReS2(1−x)Se2x porous arrays on SiO2/Si substrates for next-generation application in UV-vis photodetectors.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2022
·
IF 5.876
Saisai Ran,Hanfang Liu,Xiaolong Li,Huijie Xie,Wei Xu,Bin Li,Yu Yang,Yongming Chai,Lei Wang
Utilizing silica-rich wastes for synthesis of zeolite can realize a green production protocol and make best use of solid waste. However, industrial solid wastes usually require a complicated pretreatment process and their surface properties are underestimated for the final products. In this study, silica wastes with different acid or basic surface properties are directly used for the solvent-free synthesis of TS-1 zeolites. The states of Ti species, Si/Ti molar ratios, and morphology of final TS-1 zeolites can be controlled by the pristine surface properties and additional ammonium carbonate. TS-1 zeolite synthesized from acid silica waste exhibits lower crystallinity, abundant highly coordinating titanium species, and an elongated c-axis compared to that from basic silica waste. More importantly, TS-1 zeolite ( n S i / n T i = 30 ) synthesized from silica with acid property possesses excellent performance in the epoxidation of chloropropene with 66.2% conversion and 98.5% selectivity to epichlorohydrin, which is attributed to the presence of abundant high-coordination Ti species of mononuclear TiO 6 . Our strategy provides a novel idea for totally green synthesis of zeolite by utilizing solid wastes.
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INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
·
2022
·Vol. 50
·期 5
·1-14
·
IF 5.314
Weichao Sun,Wei Xie,Dixi Huang,Yinxing Cui,Jiaji Yue,Qifei He,Luoyong Jiang,Jianyi Xiong,Wei Sun,Qian Yi
Osteoarthritis (OA) is the most common degenerative disease affecting the joints, and inflammation appears to play a critical role in the initiation and progression of OA. Caffeic acid phenethyl ester (CAPE), a natural flavonoid compound, has anti‑inflammatory and antioxidant functions. However, its anti‑inflammatory effects on OA and the underlying mechanisms of action of CAPE in the treatment of OA remain elusive. Therefore, the present study investigated the anti‑inflammatory effects of CAPE on IL‑1β‑stimulated chondrocytes in vitro and surgically induced rat models of OA in vivo. In vitro, CAPE reduced the expression of inducible nitric oxide synthase and cyclooxygenase‑2 in IL‑1β‑stimulated chondrocytes, as well as the extracellular secretion of nitric oxide and prostaglandin E2 in the cell culture supernatants. In addition, CAPE attenuated the degradation of extracellular matrix by increasing the expression of aggrecan and collagen II, and decreasing the expression of MMP3, MMP13 and a disintegrin and metalloproteinase with thrombospondin motif‑5. Furthermore, CAPE attenuated NF‑κB signaling and activated the nuclear factor erythroid 2‑related factor 2/heme oxygenase‑1 signaling pathway in IL‑1β‑stimulated chondrocytes. In vivo, CAPE protected cartilage from destruction and delayed the progression of OA in rats. Taken together, the findings of the present study indicated that CAPE may be a potential therapeutic agent for the prevention or treatment of OA.
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EUROPEAN JOURNAL OF NEUROSCIENCE
·
2022
·Vol. 57
·期 1
·64-77
·
IF 3.698
Weimin Ni,Jie Zhang,Bing Wang,Feng Liang,Long Bao,Pengfei Li,Yan Fang
Obesity is a major health crisis in the modern society. Studies have shown that the consumption of a high-fat diet (HFD) induces hypothalamic inflammation and leptin resistance, which consequently favours body mass gain. Actin related protein 2/3 complex subunit 1 (ARPC1B), an actin-binding protein, is highly expressed in immune cells. Recent studies have shown that ARPC1B has a certain anti-inflammatory effect. While ARPC1B expression is decreased in the hypothalamus of mice fed a HFD, the role of ARPC1B in HFD-induced obesity remains unclear. Thus, we investigated whether ARPC1B up-regulation in the hypothalamic arcuate nucleus (ARC) could inhibit the development of obesity. Herein, ARPC1B overexpression lentiviral particles were stereotaxically injected into the ARC of male C57BL/6J mice (7 weeks old) fed with HFD. Overexpression of ARPC1B in the hypothalamic ARC attenuated HFD-induced ARC inflammation, reduced body-weight gain and feed efficiency. Furthermore, up-regulation of ARC ARPC1B improved the glucose tolerance and reduced subcutaneous/epididymal fat mass accumulation, which decreased the serum total cholesterol, serum triglyceride and leptin levels. In addition, upon ARPC1B overexpression in the hypothalamic ARC, intraperitoneal injection of leptin increased the phosphorylation level of signal transducer and activator of transcription 3 (STAT3), an important transcription factor for leptin's action, in the ARC of obese mice. Accordingly, we suggest that up-regulation of ARPC1B in the hypothalamic ARC may improve the HFD-induced hypothalamic inflammation and leptin resistance. Our findings demonstrate that ARPC1B is a promising target for the treatment of diet-induced obesity.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2022
·
IF 4.46
Yuan-Lin Zhang,Hong-Xia Yuan,Jian Sun,Xiu-E Feng,Jin-Hong Ren,Yuan-Biao Qiao,Qing-Shan Li
Directly targeting caveolar caveolin-1 is a potential mechanism to regulate endothelial permeability, especially during oxidative stress , but little evidence on the topic limits therapeutics discoveries. In this study, we investigated the pharmacological effect of an antioxidant LM49 (5,2′-dibromo-2,4′,5′-trihydroxydiphenylmethanoe) and its five diphenylmethanone derivatives on endothelial permeability and establish two distinct mechanisms of action. Multiplex molecular assays with theoretical modeling indicate that diphenylmethanone molecules, including LM49, directly bind the caveolin-1 steric pocket of ASN53/ARG54, ILE49/ASP50, ILE18, LEU59, ASN60, GLU48 and ARG19 residues. They also indicated dynamic binding-affinity for diphenylmethanone derivatives. First, this molecular interaction at caveolin-1 pocket inhibits its phosphorylation at TYR14 residue in H 2 O 2 -injured endothelial cell. A positive correlation was established between diphenylmethanone derivative binding-affinity and caveolin-1 phosphorylation inhibition. Inhibition of caveolin-1 phosphorylation, however, was independent of the LM49-mediated variation of protein tyrosine kinase activity, suggesting a direct blockage of adenosine triphosphate substrate diffusion into cavelion-1 structure. Second, LM49 increases the expression of cellular adhesive and tight junction proteins , VE-cadherin and occludin , in H 2 O 2 -injured cell, in a dose dependent manner. A leakage assay of fluorescein isothiocyanate-labeled dextran 40 across cell monolayer suggested improvement in endothelial barrier integrity with diphenylmethanone treatments. Our results demonstrate a direct targeting effect of caveolin-1 on endothelial permeability, and should guide the diphenylmethanone therapy against oxidative stress-induced junction dysfunction, especially at caveolar membrane invagination.
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Wen-Juan Pan,Lu-Lu Shi,Yu-Ru Ren,Cheng-Ying Yao,Yong-Ming Lu,Yan Chen
Age-associated increase in intestinal permeability is known to relate with gut microbiota dysbiosis and loss of epithelial tissue integrity. To improve healthy aging and prevent age-associated chronic disabilities, the protective potential of polysaccharides from Oudemansiella raphanipes (ORP-1) against age-associated intestinal epithelial barrier dysfunction in d -galactose-induced Caco-2 cells monolayer was investigated. In-vitro results demonstrated that ORP-1 can restore a healthy gut microbial population to handle age-related gut microbiota dysbiosis mainly by facilitating the proliferation and adhesion of probiotics Lactobacillus acidophilus ( L. acidophilus ) and Bifidobacterium bifidum ( B. bifidum ) to compete with intestinal pathogenic Escherichia coli ( E. coli ) for ecological niches and nutrition. Meanwhile, ORP-1 strengthened the intestinal structural integrity primarily by abolishing the aggravation of apoptosis and the age-associated alterations of tight junction (TJ) proteins expression in intestine. These findings highlighted that ORP-1 could be a potential functional food component with preventive utility against age-associated intestinal barrier injury.
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BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Jinzhi Liu,Mengyan Wang,Chi Zhang,Gaiping Li,Qi Shen,Lina Zou
Herein, a “signal-on” electrochemical aptasensor based on dual-output toehold mediated strand displacement reaction (TMSDR) was developed to detect cytokeratin fragment antigen 21–1 (CYFRA 21–1). First, the methylene blue (MB) labeled signal probe (SP) was combined with a long template strand (TS) to form the three-strands duplex, which was immobilized on magnetic beads. The sequence of TS was innovatively designed with two toehold zones. Thus, with the presence of target DNA and Fuel strand (F), double SP and target were released through TMSDR. Besides, the released target could participate in the next cycle, achieving the further amplification. The released SP was selected by magnetic separation, then added to the gold electrode (GE) to hybridize with the hairpin DNA. Ultimately, the target can be detected by recording the current response of the MB. Under optimized conditions, the response peak current was linear with the concentration of CYFRA 21–1 in range from 10 pM to 1 μM with detection limit of 8.079 pM. The proposed signal amplification strategy was enzyme-free in homogenous solution and the using of magnetic beads improved the recognition efficiency and simplified the experimental steps, which endowed the sensor with super-sensitivity.
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FASEB JOURNAL
·
2022
·Vol. 36
·期 9
·e22505
·
IF 5.834
Xia Wu,Xueliang Zhou,Songqing Lai,Jichun Liu,Jianwei Qi
The hallmark feature of Diabetes mellitus (DM) is hyperglycemia which can lead to excess production of reactive oxygen species (ROS) in the myocardium, contributing to diabetic cardiomyopathy (DCM). Nuclear factor erythroid2-related factor2 (Nrf2), a transcriptional activator, enhances its ability to resist oxidative stress by activating multiple downstream anti-oxidants, anti-inflammatory proteins, and detoxifying enzymes. However, the mechanism of Nrf2 signaling in HG-induced DCM is unclear. In this study, we used HG pretreated H9c2 cells as the experimental basis in vitro, and established a high fat-diet, streptozotocin (STZ) induced Type 2 diabetic rat model in vivo. Meanwhile, we used shRNA-Nrf2 and curcumin (CUR) (as an activator) to affect H9c2 cells, to verify the role of the Nrf2 signaling pathway in DCM. The results showed that the excessive production of ROS caused by HG, which could inhibit the activation of Nrf2-related signaling, resulting in a decrease in cell energy metabolism and an increase in cell apoptosis. Surprisingly, we found that the activation of the Nrf2 signaling pathway significantly increased cardiomyocyte viability, reduced ROS formation, increased antioxidant enzyme activity, and inhibited cardiomyocyte apoptosis. In conclusion, these findings conclusively infer that CUR activation of the Nrf2/HO-1 signaling pathway exerts myocardial protection by reducing ROS formation.
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BIOORGANIC CHEMISTRY
·
2022
·
IF 5.307
Hua-Tao Liu,Chun-Yue Weng,Shen-Yuan Xu,Shu-Fang Li,Ya-Jun Wang,Yu-Guo Zheng
Traditional screening methods of enzyme engineering often require building large mutant libraries to screen for potentially beneficial sites, which are often time-consuming and labor-intensive with low mining efficiency. In this study, a novel enzyme engineering strategy was established to modify carbonyl reductase Ls CR for the synthesis of (1 S )-2-chloro-1-(3,4-difluorophenyl) ethanol (( S )-CFPL), which is a key intermediate of anticoagulant drug ticagrelor. The strategy was developed by combining HotSpot, FireProt and multiple sequence alignment, resulting in the construction of a “small and smart” mutant library including 10 mutations. Among them, 5 mutations were positive, resulting in a 50% mining accuracy of beneficial sites. Finally, a highly active mutant Ls CR M3 (N101D/A117G/F147L) was obtained by further screening through saturation mutation and iterative mutation. Compared with wild type (WT) Ls CR, the catalytic activity of Ls CR M3 was increased by 4.7 times, the catalytic efficiency k cat / K M value was increased by 2.9 times, and the half-life t 1/2 at 40 °C was increased by 1.3 times. Due to the low aqueous solubility of the substrate 2-chloro-1-(3,4-difluorophenyl) ethanone (CFPO), isopropanol was used as not only the co-substrate but also co-solvent. In the presence of 40% ( v / v ) isopropanol, Ls CR M3 completely reduced 400 g/L CFPO to enantiomerically pure CFPL (99.9%, e.e. ) in 11 h with a space–time yield (STY) as high as 809 g/L∙d.
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Zicheng Chen,Xiangyang He,Jing Ge,Guangyuan Fan,Lanhe Zhang,Ashak Mahmud Parvez,Guanhua Wang
Nanofibrillated cellulose (NFC) has presented great potential as supports for functional membrane materials owing to its unique advantages. In this work, NFC-supported MOF hierarchically porous membranes were synthesized by anchoring HKUST-1 (copper 1,3,5-benzenetricarboxylate open-framework) on NFC membrane through a green DMF-free (N,N-Dimethylformamide) method at room temperature and afterward, the as-prepared composite membrane was used to remove formaldehyde from the air. The addition of HKUST-1 particles significantly increased the specific surface area of NFC membrane without affecting the NFC properties. Meanwhile, the nucleation and growth process of HKUST-1 on the NFC membrane could be effectively regulated, which further controlled the morphology of HKUST-1 as well as the anchored position of HKUST-1. When HKUST-1 was anchored on the interior and surface of the membrane, HKUST-1 reduced the compactness of the arrangement between fibers, which resulted in a hierarchical porous structure and then exposed more active adsorption sites of HKUST-1 particles. Thus, the flexible composite membranes exhibited effective formaldehyde adsorption from a low formaldehyde concentration environment (3.0 mg/m 3 ). Under the optimum conditions, the maximum adsorption capacity of the HKUST-1@NFC-1 for formaldehyde reached 378.09 mg/g (based on HKUST-1 wt), which was considerably higher than support-free HKUST-1 powders. Consequently, the present work offers a green and controllable route to prepare NFC-supported MOF composite membranes with highly effective formaldehyde adsorption capacity and thus provides a promising option for NFC application as a flexible adsorbent support.
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MOLECULES
·
2022
·Vol. 27
·期 13
·4129
·
IF 4.927
Taiyu Wang,Hailong Yang,Jie Yang,Ningjie Guo,Guodong Wu,Xueyu Xu,Ming An
A novel method of ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed for the identification and quantification of four potential genotoxic impurities (PGIs) in the active pharmaceutical ingredients of TSD-1, a novel P2Y12receptor antagonist. Four PGIs were named, 4-nitrobenzenesulfonic acid, methyl 4-nitrobenzenesulfonate, ethyl 4-nitrobenzenesulfonate, and isopropyl 4-nitrobenzenesulfonate. Following the International Conference of Harmonization (ICH) guidelines, this methodology is capable of quantifying four PGIs at 15.0 ppm in samples of 0.5 mg/mL concentration. This validated approach presented very low limits (0.1512–0.3897 ng/mL), excellent linearity (coefficients > 0.9900), and a satisfactory recovery range (94.9–115.5%). The method was sufficient in terms of sensitivity, linearity, precision, accuracy, selectivity, and robustness and, thus, has high practicality in the pharmaceutical quality control of TSD-1.Keywords:UPLC-MS/MS;genotoxic impurities;TSD-1;active pharmaceutical ingredients;quantitation;method validation
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