ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 9
·1687-1698
·
IF 4.3
Liu Cheng,Zheng Jingtian,Zhang Benxing,Zhong Xianhua,Wang Wei,Li Zhangyong
The Fenton reaction is one of the most effective methods for treating organic wastewater, which is extremely harmful to humans but difficult to treat. However, finding simple, low-cost, and efficient catalysts for the Fenton reaction remains a challenge. In this study, a BSA-Cu 3 (PO 4 ) 2 hybrid nanoflower (NF) was synthesized to investigate its peroxidase-like activity for the treatment of organic wastewater. Its morphology, composition, and crystallization had been fully studied and the results confirmed that the NFs were successfully prepared. Subsequently, the origin of the peroxidase-like activity of the NFs was further analyzed, with the results suggesting two reasons: (i) the transformation between Cu(I) and Cu(II) and (ii) nano-effects. Additionally, Congo red was selected as the organic pollutant to simulate the decolorization of wastewater. After 3 h, the decolorization efficiency reached 96%. Furthermore, the NFs exhibited good storage performance, maintaining approximately 90% relative activity after storage for 30 days. In summary, the NFs have great application prospects in the treatment of organic wastewater. Graphical abstract
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Molecular Catalysis
·
2023
·
IF 4.6
Tongyun Zhang,Chengping Zhang,Xiaoxun Ma,Hengdao Quan
3,3,3-trifluoropropyne (HFY-2223tz) is a novel refrigerant with great physicochemical properties. Though preparation of HFY-2223tz from halogenated olefins is theoretically effective, it remains great challenges for dehydrohalogenation from halogenated olefins, limiting the detailed knowledge. Here, we established a facile investigation to explore the gas-phase dehydrochlorination reaction of 2‑chloro-3,3,3-trifluoropropene (HCFO-1233xf) over various halides (Na, K, Rb, Cs) supported active carbon (AC) catalysts. The CsF was concluded as the optimal active component with best dehydrochlorination selectivity and conversion of HCFO-1233xf. The CO 2 -TPD results illustrated that dehydrochlorination of HCFO-1233xf proceeded through E1cB mechanism. Mechanism study revealed the evolution of side-products including ( Z / E )-1,3,3,3-tetrafluoropropylene, 2,3,3,3-tetrafluoropropylene, ( Z / E )-1‑chloro‑3,3,3-trifluoroproene, and 3,3,3-trifluoroprop-1-ene, etc. However, observable deactivation of CsF catalyst was noticed, which, according to combined XRD and XPS results and density functional theory (DFT) calculations, was attributed to the F/Cl exchange between HCFO-1233xf and catalyst, leading to rapid fluorine consumption. This work thus provides basic knowledge about heterogeneous catalytic synthesis of HFY-2223tz from dehydrohalogenation of halogenated olefins.
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Signal Transduction and Targeted Therapy
·
2023
·Vol. 8
·期 1
·1-12
·
IF 39.3
Gu Yangzhuo,Yang Jingyun,He Cai,Zhao Tingmei,Lu Ran,Liu Jian,Mo Xianming,Wen Fuqiang,Shi Huashan
mRNA vaccines have emerged rapidly in recent years as a prophylactic and therapeutic agent against various diseases including cancer and infectious diseases. Improvements of mRNA vaccines have been underway, among which boosting of efficacy is of great importance. Pam2Cys, a simple synthetic metabolizable lipoamino acid that signals through Toll-like receptor (TLR) 2/6 pathway, eliciting both humoral and cellular adaptive immune responses, is an interesting candidate adjuvant. To investigate the enhancement of the efficacies of mRNA vaccines by Pam2Cys, the adjuvant was incorporated into mRNA-lipid nanoparticles (LNPs) to achieve co-delivery with mRNA. Immunization with the resulting mRNA-LNPs (Pam2Cys) shaped up the immune milieu in the draining lymph nodes (dLNs) through the induction of IL-12 and IL-17, among other cytokines. Antigen presentation was carried out mainly by migratory and dLN-resident conventional type 2 DCs (cDC2s) and significantly more potent antitumor responses were triggered in both prophylactic and therapeutic tumor models in a CD4 + and CD8 + T cell-dependent fashion. Accompanying memory antitumor immunity was also established. Moreover, the vaccine also stimulated much more robust humoral and cellular immunity in a surrogate COVID-19 prophylactic model. Last but not the least, the new vaccines exhibited good preliminary safety profiles in murine models. These facts warrant future development of Pam2Cys-incorporated mRNA vaccines or relevant mRNA therapeutics for clinical application.
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ACS Catalysis
·
2023
·
IF 12.9
Zhe-Ming Wu,Feng Xie,Wen Zheng,Chang-Feng Liu,Chao-Ping Lin,Ren-Chao Zheng,Yu-Guo Zheng
Amidases are robust biocatalysts for the industrial synthesis of carboxylic acids. Amidase-catalyzed hydrolysis of 2-chloronicotinamide is regarded as a promising route for 2-chloronicotinic acid production, which is an important building block for agrochemicals and pharmaceuticals. However, the o-substituted Cl of the pyridine ring caused a strong electronic effect and steric hindrance. Engineering access tunnels is one of the most efficient strategies to improve catalytic efficiency by adjusting the transfer efficiency of ligands. Herein, we engineered a twisted access tunnel of amidase and the mutant PaAmiM1A378V+V402L+L403V was achieved, which exhibited increased catalytic efficiency toward 2-chloronicotinamide. Tunnel analysis, molecular dynamics simulations, and quantum mechanical calculations revealed that the straightened tunnel significantly improved the transport efficiency and simultaneously facilitated substrate binding and nucleophilic attack. With the best mutant as a biocatalyst, 1330 mM product was accumulated within 2 h and a high space-time-yield of 2515 gproduct L–1 d–1 was achieved, which is the highest ever reported. These results lay the foundation for industrial production of 2-chloronicotinic acid and give important guidance for engineering amidases into versatile biocatalysts.
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Solar RRL
·
2023
·Vol. 7
·期 17
·2300318
·
IF 7.9
Bowen Liu,Xinan Shi,Wei Shao,Jiaxin Gao,Chenxi Zhao,Fuyan Chen,Dongdong Shen,Bingsuo Zou,Daocheng Pan
Cu(In,Ga)(S,Se) 2 (CIGSSe) solar cells are one of the most promising thin film photovoltaics with a record efficiency of 23.6%. However, the biggest drawback to CIGSSe solar cells is their high material cost, partially resulting from relatively rare and expensive indium and gallium. Therefore, improving the utilization of indium and gallium and reducing their wastage can greatly lower the cost of CIGSSe thin film solar cells. Inkjet printing is a facile, cost-effective, and low-waste deposition technology, which is particularly suitable for low-cost and large-area fabrication of CIGSSe solar cells. Herein, a novel and green ionic liquid-assisted ink to fabricate highly efficient CIGSSe solar cells is developed. The material utilization of inkjet printing can be remarkably improved compared to the conventional vacuum-based deposition approach and spin-coating solution approach. The CIGS printable ink is prepared by dissolving copper acetate, indium acetate, gallium nitrate, and thiourea into ethanol with the assistance of n -butylammonium butyrate ionic liquid. Ionic liquid-assisted CIGS ink has a nearly zero wetting angle and a tunable viscosity, which enable to deposit a flat and continuous CIGS thin film by an inkjet printing method. An encouraging power conversion efficiency of 15.22% has been achieved for inkjet-printed CIGSSe solar cells.
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International Journal of Biological Sciences
·
2022
·Vol. 18
·期 16
·6008-6019
·
IF 10.75
Li Chao,Lv Li-fang,Qi-Li Mu-ge,Yang Rui,Wang Yu-jing,Chen Shuang-shuang,Zhang Ming-xiu,Li Tian-yu,Yu Tong,Zhou Yu-hong,Liang Hai-hai,Shan Hong-li,Li Xue-lian
Cardiac fibrosis is one of the common pathological processes in many cardiovascular diseases characterized by excessive extracellular matrix deposition. SerpinE2 is a kind of protein that inhibits peptidase in extracellular matrix and up-regulated tremendously in mouse model of cardiac fibrosis induced by pressure-overloaded via transverse aortic constriction (TAC) surgery. However, its effect on cardiac fibroblasts (CFs), collagen secretion and the underlying mechanism remains unclear. In this study, DyLight® 488 green fluorescent dye or His-tagged proteins were used to label the exogenous serpinE2 protein. It was showed that extracellular serpinE2 translocated into CFs by low-density lipoprotein receptor-related protein 1 (LRP1) and urokinase plasminogen activator receptor (uPAR) of cell membrane through endocytosis. Knockdown of LRP1 or uPAR reduced the level of serpinE2 in CFs and down-regulated the collagen expression. Inhibition of the endocytosis of serpinE2 could inhibit ERK1/2 and β-catenin signaling pathways and subsequently attenuated collagen secretion. Knockdown of serpinE2 attenuates cardiac fibrosis in TAC mouse. We conclude that serpinE2 could be translocated into cardiac fibroblasts due to endocytosis through directly interact with the membrane protein LRP1 and uPAR, and this process activated the ERK1/2, β-catenin signaling pathways, consequently promoting collagen production.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2022
·Vol. 39
·期 2
·219-223
·
IF 2.726
Xiao Long,Wu Huirong,Zhang Yong,Sun Hao,Zhang Wenchao,Lyu Fenglei,Deng Zhao,Peng Yang
O xygen evolution reaction(OER) plays a key role in the electrochemical conversion and storage processes, but the sluggish kinetics of OER strongly impedes its large-scale applications. We herein reported the in situ growth of Fe-benzenedicarboxylate(Fe-BDC) on Co(OH) 2 nanoplates[Fe-BDC/Co(OH) 2 ] that showed remarkably enhanced OER activity than the pristine Co(OH) 2 . The incorporation of Fe species could enhance the intrinsic OER activity of Co and BDC could increase the electrochemically active surface area(ECSA), thus resulting in dramatically enhanced OER activity. In situ Raman spectroscopy characterization disclosed that Fe-CoOOH reconstructed from Fe-BDC/Co(OH) 2 was the real active site for OER. This work highlights the significance of rational tailoring of the nanostructure and electronic structure of Co(OH) 2 and provides more opportunities for its widespread applications.
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Nanomaterials
·
2022
·Vol. 12
·期 22
·3972
·
IF 5.719
Xue-Zhi Song,Yu-Hang Zhao,Fan Zhang,Jing-Chang Ni,Zhou Zhang,Zhenquan Tan,Xiao-Feng Wang,Yanqiang Li
The oxygen evolution reaction (OER) is kinetically sluggish due to the limitation of the four-electron transfer pathway, so it is imperative to explore advanced catalysts with a superior structure and catalytic output under facile synthetic conditions. In the present work, an easily accessible strategy was proposed to implement the plant-polyphenol-involved coordination assembly on Co(OH)2nanosheets. A TA-Fe (TA = tannic acid) coordination assembly growing on Co(OH)2resulted in the heterostructure of Co(OH)2@TA-Fe as an electrocatalyst for OER. It could significantly decrease the overpotential to 297 mV at a current density of 10 mA cm−2. The heterostructure Co(OH)2@TA-Fe also possessed favorable reaction kinetics with a low Tafel slope of 64.8 mV dec−1and facilitated a charge-transfer ability. The enhanced electrocatalytic performance was further unraveled to be related to the confined growth of the coordination assembly on Co(OH)2to expose more active sites, the modulated surface properties and their synergistic effect. This study demonstrated a simple and feasible strategy to utilize inexpensive biomass-derived substances as novel modifiers to enhance the performance of energy-conversion electrocatalysis.Keywords:Co(OH)2;tannic acid;electrocatalyst;OER;coordination assembly
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PLoS One
·
2022
·Vol. 17
·期 10
·e0275428
·
IF 3.752
Zhi-Bin Dong,Yu-Jia Wang,Meng-Lin Cheng,Bo-Jun Wang,Hong Lu,Hai-Li Zhu,Ling Liu,Min Xie
Oxaliplatin (OXA) is a third-generation platinum compound with clinical activity in multiple solid tumors. Due to the repetition of chemotherapy cycle, OXA-induced chronic neuropathy presenting as paresthesia and pain. This study explored the neuropathy of chemotherapy pain and investigated the analgesic effect of 2-bromopalmitate (2-BP) on the pain behavior of OXA-induced rats. The chemotherapy pain rat model was established by the five consecutive administration of OXA (intraperitoneal, 4 mg/kg). After the establishment of OXA-induced rats, the pain behavior test, inflammatory signal analysis and mitochondrial function measurement were conducted. OXA-induced rats exhibited mechanical allodynia and spinal inflammatory infiltration. Our fluorescence and western blot analysis revealed spinal astrocytes were activated in OXA rats with up-regulation of astrocytic markers. In addition, NOD-, LRR- and pyrin domain-containing 3 (NLRP3) inflammasome mediated inflammatory signal cascade was also activated. Inflammation was triggered by dysfunctional mitochondria which represented by increase in cyclooxygenase-2 (COX-2) level and manganese superoxide dismutase (Mn-SOD) activity. Intrathecally injection of 2-BP significantly attenuated dynamin-related protein 1 (Drp1) mediated mitochondrial fission, recovered mitochondrial function, suppressed NLRP3 inflammasome cascade, and consequently decreased mechanical pain sensitivity. For cell research, 2-BP treatment significantly reversed tumor necrosis factor-α (TNF-α) induced mitochondria membrane potential deficiency and high reactive oxygen species (ROS) level. These findings indicate 2-BP decreases spinal inflammation and relieves OXA-induced neuropathic pain via reducing Drp1-mediated mitochondrial dysfunction.
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POLYMER ENGINEERING AND SCIENCE
·
2022
·Vol. 62
·期 11
·3831-3840
·
IF 2.573
Sen Jiang,Liyan Wang,Rongrong Zheng,Yunsheng Chong,Yanming Chen
The poly(butylene succinate) (PBS) copolyesters modified by 2-methyl-1,3-propanediol (MPO), that is poly(butylene succinate-co-methyl propylene succinate) (PBMS), were synthesized by direct esterification-melt polycondensation method. 1 H NMR showed the PBMS molecules contain two repeating structural units, the butylene succinate (BS) and the 2-methyl-1,3-propylene succinate (MS). The crystalline properties of PBMS were characterized by DSC, POM, and XRD. The results showed the relative crystallinity of PBMS decreases from 45.12% to 38.2%, the size of spherulites also decreases, and the crystallization ability becomes weaker compared with PBS. The water contact angle test proved the surface hydrophilicity of PBMS is improved. The impact strength of PBMS increases from 3.9 to 24.6 kJ/m 2 , and its elongation at break increases from 10.5% to 71.2%, and the transition from brittle fracture to ductile fracture is achieved. The study of enzymatic degradation found that the enzymatic degradation rate of PBMS copolyesters increases significantly with the increase of MPO content. Within 25 days of degradation, the weight loss rate of PBS after degradation is 38.18%, while that of PBMS reaches 100%. Finally, it was also observed the enzymatic degradation of PBS and PBMS starts from the amorphous region and abides by the regular pattern from the periphery to the middle and from the surface to the interior.
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Advanced Materials Interfaces
·
2022
·Vol. 9
·期 35
·2201688
·
IF 6.389
Liu-Qin Zhang,Hua-Li Li,Xiao-Hu Zhang,Qiu-Ping Li,Shu-Wen Luo,Fa-Qian Liu
Metal–organic gels or metallogels (MOGs), which usually made up with metal ions and organic ligands, are promising in the area of medicine, environment, and biology. Herein, a new metal–organic gel containing Cu + (Cu-MOG-5) is prepared at room temperature using 2-amino-5-mercapto-1,3,4-thiadiazole (AMT) and CuI. Considering the excellent low surface energy characteristics of polydimethylsiloxane (PDMS), the as-prepared Cu-MOG-5 is infused into porous PDMS to obtain Cu-MOG-5/PDMS coating, which combines the bactericidal properties of Cu + containing hydrogels and the low surface energy of PDMS. The morphology of the Cu-MOG-5 and Cu-MOG-5/PDMS coating is investigated by FE-SEM, and the structure of the new Cu-MOG-5 is verified by FT-IR, 1 H NMR, XPS, EDS, and elemental analysis. The antibiofouling properties are assessed using Escherichia coli ( E. coli ) and mussel. The results show that the newly prepared Cu-MOG-5/PDMS coating exhibits superior antibiofouling performance because of the synchronous action of hydrogel including Cu + and PDMS.
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Nano Research
·
2022
·Vol. 16
·期 4
·5383-5390
·
IF 10.269
Mao Guobin,Yang Yang,Cao Shijie,Ye Silu,Li Yifang,Zhao Wei,An Hongwei,Liu Yingxia,Dai Junbiao,Ma Yingxin
The global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus has necessitated rapid, easy-to-use, and accurate diagnostic methods to monitor the virus infection. Herein, a ratiometric fluorescence enzyme-linked immunosorbent assay (ELISA) was developed using Si-fluorescein isothiocyanate nanoparticles (FITC NPs) for detecting SARS-CoV-2 nucleocapsid (N) protein. Si-FITC NPs were prepared by a one-pot hydrothermal method using 3-aminopropyl triethoxysilane (APTES)-FITC as the Si source. This method did not need post-modification and avoided the reduction in quantum yield and stability. The p-nitrophenyl (pNP) produced by the alkaline phosphatase (ALP)-mediated hydrolysis of p-nitrophenyl phosphate (pNPP) could quench Si fluorescence in Si-FITC NPs via the inner filter effect. In ELISA, an immunocomplex was formed by the recognition of capture antibody/N protein/reporter antibody. ALP-linked secondary antibody bound to the reporter antibody and induced pNPP hydrolysis to specifically quench Si fluorescence in Si-FITC NPs. The change in fluorescence intensity ratio could be used for detecting N protein, with a wide linearity range (0.01–10.0 and 50–300 ng/mL) and low detection limit (0.002 ng/mL). The concentration of spiked SARS-CoV-2 N protein could be determined accurately in human serum. Moreover, this proposed method can accurately distinguish coronavirus disease 2019 (COVID-19) and non-COVID-19 patient samples. Therefore, this simple, sensitive, and accurate method can be applied for the early diagnosis of SARS-CoV-2 virus infection.
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