Frontiers in Microbiology
·
2021
·
IF 5.64
Zhao Shuxue,Chen Xi,Sun Qianshu,Wang Fei,Hu Chunhui,Guo Lizhong,Bai Jie,Yu Hao
Indole-3-acetic acid (IAA), known as a common plant hormone, is one of the most distributed indole derivatives in the environment, but the degradation mechanism and cellular response network to IAA degradation are still not very clear. The objective of this study was to elucidate the molecular mechanisms of IAA degradation at the protein level by a newly isolated strain Pseudomonas sp. LY1. Label-free quantitative proteomic analysis of strain LY1 cultivated with IAA or citrate/NH4Cl was applied. A total of 2,604 proteins were identified, and 227 proteins have differential abundances in the presence of IAA, including 97 highly abundant proteins and 130 less abundant proteins. Based on the proteomic analysis an IAA degrading (iad) gene cluster in strain LY1 containing IAA transformation genes (organized as iadHABICDEFG), genes of the β-ketoadipate pathway for catechol and protocatechuate degradation (catBCA and pcaABCDEF) were identified. The iadA, iadB, and iadE-disrupted mutants lost the ability to grow on IAA, which confirmed the role of the iad cluster in IAA degradation. Degradation intermediates were analyzed by HPLC, LC-MS, and GC-MS analysis. Proteomic analysis and identified products suggested that multiple degradation pathways existed in strain LY1. IAA was initially transformed to dioxindole-3-acetic acid, which was further transformed to isatin. Isatin was then transformed to isatinic acid or catechol. An in-depth data analysis suggested oxidative stress in strain LY1 during IAA degradation, and the abundance of a series of proteins was upregulated to respond to the stress, including reaction oxygen species (ROS) scavenging, protein repair, fatty acid synthesis, RNA protection, signal transduction, chemotaxis, and several membrane transporters. The findings firstly explained the adaptation mechanism of bacteria to IAA degradation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TETRAHEDRON
·
2021
·
IF 2.457
Qin He,Fangdi Dong,Longjiang Xing,Huahong He,Xiaoping Chen,Huaqian Wang,Shaomin Ji,Yanping Huo
A series of perylene derivatives bearing electron-donating group (amino) and electron-withdrawing group (nitro, maleimide) at the 1- and 3-position have been synthesized. Interestingly, 3-monosubstituted perylenes shown different photophysical properties compared with 1-monosubstituted perylenes. 3-nitroperylene ( 3-NO ) attained 80.62% photoluminescence quantum yield ( Φ PL ) in toluene which is higher than 3-aminoperylene ( 3-NH , Φ PL = 71.70%) and 1-aminoperylene (1-NH, Φ PL = 48.04%), but for 1-nitroperylene ( 1-NO ), no fluorescence in any solvent were observed. The calculated ground-state geometries of 3-monosubstituted perylenes actually correspond to nearly planar structures, but the molecules substituted at the 1-position all have a twisted structure. Among them, 3-NO had a great π-conjugated system, resulting in the allowed ππ∗ fluorescence. In contrast, the twisting structure of 1-NO enhanced nonradiative decay pathways, coupled with the electron-withdrawing effect of the nitro group, which can explain the non-luminescence of 1-NO . Furthermore, the moleculars with maleimide group were used as “off-on” fluorescent probes and successfully used for imaging biothiols in living H1299 lung cancer cells. The fluorescence of probe 2 (substitutes at 3-position of perylene) afforded a 188-fold intensity increase after reaction with thiol which is much higher than (65-fold) probe 1 (substitutes at 1-position) because of the better π-conjugated structure. We envision that the investigation on the effects of substitute at 1-and 3-positions of perylene may be helpful for a rational design and application of highly fluorescent molecule base on perylene.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 43
·26502-26508
·
IF 3.361
Meirong Fu,Mingqiang Li,Yingying Zhao,Yunxiang Bai,Xingzhong Fang,Xiaolong Kang,Min Yang,Yanping Wei,Xia Xu
Precious metal nanometric catalysts are widely used in the removal of harmful substances. In the process of synthesis and catalytic reaction, it is particularly important to study green and simple synthesis methods and high catalytic efficiency. In this paper, a green one-step method was used to synthesize the Fe(OH)3/Fe2O3@Au composite catalyst, in which Au was single atom-dispersed. The removal of 4-nitrophenol (4-NP), a typical dangerous chemical widely existing in factory waste gas, waste water and automobile exhaust gas, was catalysed by Fe(OH)3/Fe2O3@Au. The catalytic performance of Fe(OH)3/Fe2O3@Au with different synthesis conditions (different amounts of MES, NaBH4, FeSO4, Au and Pt) on the 4-NP reduction reaction were systematically studied. Finally, the stability and recyclability of Fe(OH)3/Fe2O3@Au composite nanocatalyst were investigated thoroughly.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2021
·
IF 5.565
Yanqing Zhou,Yili Wang,Shuoxun Dong,Haotian Hao,Junyi Li,Chenyang Liu,Xiaolin Li,Yao Tong
Cationic hydrogels have received great attention to control eutrophication and recycle phosphate. In this study, a type of La(OH) 3 loaded magnetic MAPTAC-based cationic hydrogel (La(OH) 3 @MMCH) was developed as a potential adsorbent for enhanced phosphate removal from aqueous environment. La(OH) 3 @MMCH exhibited high adsorption capacity of 105.72±5.99 mg P/g, and reached equilibrium within 2 hr. La(OH) 3 @MMCH could perform effectively in a wide pH range from 3.0 to 9.0 and in the presence of coexisting ions (including SO 4 2− , Cl − , NO 3 − , HCO 3 − , SiO 4 4− and HA). The adsorption-desorption experiment indicated that La(OH) 3 @MMCH could be easily regenerated by using NaOH-NaCl as the desorption agent, and 73.3% adsorption capacity remained after five cycles. Moreover, La(OH) 3 @MMCH was employed to treat surface water with phosphate concentration of 1.90 mg/L and showed great removal efficiency of 95.21%. Actually, MMCH showed high surface charge density of 34.38-59.38 meq/kg in the pH range from 3.0 to 11.0 and great swelling ratio of 3014.57% within 24 h, indicating that MMCH could produce the enhanced Donnan membrane effect to pre-permeate phosphate. Furthermore, the bifunctional structure of La(OH) 3 @MMCH enabled it to capture phosphate through electrostatic attraction and ligand exchange . All the results prove that La(OH) 3 @MMCH is a promising adsorbent for eutrophication control and phosphate recovery.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Experimental and Therapeutic Medicine
·
2021
·Vol. 22
·期 3
·1-10
·
IF 2.447
Jing Li,Yu-Shan Mao,Fen Chen,Dong-Xia Xia,Tin-Qi Zhao
Insulin resistance is the main sign of type 2 diabetes mellitus and is often accompanied by the infiltration of inflammatory factors. These inflammatory factors are mainly produced and secreted by macrophages. The purpose of the current study was to explore the relationship between macrophages and insulin resistance, and to determine its underlying mechanism. The insulin resistance of macrophages was induced by palmitic acid (PA) in vitro. The glucose uptake rate of macrophages, the expression levels of inflammatory cytokines and the expression levels of insulin resistance‑related proteins were detected. The protein expression levels of Krüppel‑like factor 4 (KLF4), toll‑like receptor 4 (TLR4), NF‑κB and Galectin‑3 (Gal‑3) were detected via western blotting and recovery experiments were performed by combining the Gal‑3 and TLR4 inhibitors GB1107 and TAK242. The results revealed that PA‑induced macrophages demonstrated insulin resistance. Additionally, KLF4 protein was inhibited and the sugar uptake rate was significantly lower than that of the control group. Western blotting and immunofluorescence assays revealed that the expression of Gal‑3 in PA‑induced macrophages was significantly upregulated. The addition of the Gal‑3 inhibitor GB1107 significantly increased glucose utilization and reduced insulin resistance in PA‑treated cells. Inhibitor of TLR4 inhibited the protein expression level of the TLR4/NF‑κB pathway. In conclusion, PA promoted the TLR4/phosphorylated‑NF‑κB signaling pathway by inhibiting KLF4, promoted the upregulation of Gal‑3 expression and improved the insulin resistance of macrophages.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
Rujie Yang,Fangbo Xia,Huanxing Su,Jian-Bo Wan
n-3 polyunsaturated fatty acids (PUFAs) and their metabolites play the crucial role in a wide range of physiologic and pathologic processes, including cardiovascular, neurodegenerative diseases, and inflammation-associated disorders. However, the quantitative analysis of n-3 PUFAs and their metabolites, oxylipins , is obstructed by high structural similarity, poor ionization efficiency and low abundance. In this study, a sensitive method was developed to quantify 28 n-3 PUFAs/oxylipins using chemical isotope labeling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Standards labeled with cholamine- d 9 were used as one-to-one internal standards to achieve accurate quantification. The cholamine- d 0 -derivatized biological samples were mixed with cholamine d 9 -labeled standards for LC-MS/MS with multiple reaction monitoring. After cholamine derivatization, both MS sensitivity and chromatographic performance of n-3 PUFAs/oxylipins were substantially improved. Furthermore, the relationship between retention time and substituent position of regioisomers, and their fragmentation patterns were investigated, which may facilitate the identification of unknown oxylipins. Additionally, the developed method was applied to quantify the target n-3 PUFAs/oxylipins in serum and brain tissue from fish oil-supplemented mice, which exhibited its great potential and practicability. Collectively, this sensitive and reliable method may facilitate the elucidation of the roles of n-3 PUFAs/oxylipins in the physiological and pathological processes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POWDER TECHNOLOGY
·
2021
·
IF 5.134
Chendong Zhang,Hangchen Li,Xinxin Guo,Shunchao Li,Han Zhang,Xuhai Pan,Min Hua
A new type of dry powder fire extinguishing agent was prepared, which was called composite superfine dry powder with Aluminum Trihydrate (CSFP-ATH). The CSFP-ATH was characterized by laser particle size analysis , scanning electron microscope (SEM), thermal gravity analyzer (TGA), transform infrared spectroscopy(FTIR) analyzer and differential scanning calorimetry (DSC). The extinguishing performance of different samples was examined in a small-scale experimental platform for the liquid hydrocarbon fire. The tests were proved that the fire-extinguishing performance was as follows: CSFP-ATH > superfine ABC dry powder(SFP) > commercial ABC dry powder(CDP). Besides, the mechanism, kinetics and thermochemical changes in the reaction process were calculated by Gaussian simulation, and the reaction path of the CSFP-ATH with free radicals in the flame was predicted. Experimental and theoretical studies have been proved that the addition of Al(OH) 3 can promote the fire extinguishing performance of superfine ABC dry powder.
✓ 已验证
产品关联
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2021
·
IF 2.495
Qian-Qian Huang
A novel tetranuclear nickel(II)-flavonolate complex [Ni II 4 (fla) 4 (CH 3 O) 4 (H 2 O) 4 ] (flaH: 3-Hydroxyflavone, flavonol) was synthesized. Its crystal structure and spectroscopic features have been investigated in details by physicochemical methods and single crystal X-ray diffraction. Different from other mononuclear metal-flavonolate complexes, this complex demonstrates a cluster molecular structure which has four Ni II centers, and each Ni II centre is coordinated by a bidentate flavonolate, three methanol and a water molecule. The crystal of this complex is tetragonal crystal system: space group I 41/a, a = 24.4361(14), b = 24.4361(14), c = 12.7972 (14), α = β = γ = 90 ° , V = 7641.5 (12), Z = 4. The complex can react with O 2 , in other words, it has enzymatic reactivity just like enzyme-substrate model of quercetin 2,3-dioxygenase, and the reaction products are similar with the other reported enzyme-substrate models.
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Chemistry
·
2021
·
IF 8.301
J. Zeng,H. Kong,X. Du,Q. Xu,F. Jiang,B. Li,M. Yu
PBO fiber is one of the most promising reinforcements in resin matrix composite because of its excellent mechanical properties . However, the inert and smooth surfaces make it the poor interface adhesion with resin matrix, which seriously limits the application in composites. In this article, we report a method to modify the surface of PBO fibers with 2,2-Bis (3-amino-4-hydroxyphenyl) hexafluoropropane（BisAPAF）in supercritical CO 2 to enhance interfacial properties . Chemical structures , surface elemental composition and functional groups, and surface morphology were characterized by FT-IR spectrometer, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM), respectively. The mechanical properties of the samples were tested by a tensile tester. Static contact angle and microdebonding tests were used to characterize the wetting ability and interfacial shear strength (IFSS) of the fiber and epoxy resin. The results showed that the BisAPAF could be solved in scCO 2 and introduced more groups, –NH 2 , –OH, and –CF 3 on the fiber surface, resulting in the mechanical properties and the wettability of PBO fiber slightly improved. Moreover, the fiber surface roughness was also increased obviously. The IFSS between the modified PBO fiber and epoxy resin increased from 8.18 MPa to 31.4 MPa when the treating pressure was 14 MPa. In general, the method to modify PBO fibers surface using BisAPAF in scCO 2 can effectively improve their interfacial properties.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yawen Wu,Xiaolong Chen,Xianfeng Wang,Mei Yang,Faliang Xu,Changjun Hou,Danqun Huo
The effective detection of CA15-3 is of great significance for the postoperative monitoring and early diagnosis of breast cancer. Here, a fluorescent biosensor based on the fluorescence resonance energy transfer (FRET) between the prismatic hollow Metal-polydopamine frameworks (PHMPF) and 6-carboxyfluorescein (FAM)-labeled protein aptamer (FAM-Apt) was established to detect CA15-3. Characterization of SEM, TEM, EDS, FT-IR, XRD and XPS confirmed that the synthesized PHMPF was a polyhedral prismatic hollow organometallic frame containing the elements of C, N, O, and Co. PHMPF can quench the fluorescent group modified on the aptamer efficiently. Under optimized conditions, the proposed fluorescent biosensor can detect CA15-3 low to 2.56 × 10 −5 U mL −1 . In addition, the excellent selectivity and spiked experiments indicate the proposed biosensor owns potential applications for the clinical diagnosis of tumor markers in body fluids.
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
·
2020
·Vol. 25
·期 4
·589-598
·
IF 2.213
He Jinjia,Chen Yi,Dai Luyao,Yao Jiachao,Mei Yu,Hrynsphan Dzmitry,Tatsiana Savitskaya,Chen Jun
Acrylic acid is used in various industrial applications but inflicts harm to human health and causes environmental pollution. A new bacterium, identified as Rhodococcus ruber JJ-3, was isolated, which can degrade high concentrations of acrylic acid rapidly and completely. Experimental results showed that the strain can achieve complete degradation of 1000 mg·L −1 acrylic acid in 11 h under the following conditions: pH 7, temperature 35°C, and inoculation quantity 15%. A high concentration of acrylic acid (2000 mg·L −1 ) can be completely removed in 28 h. According to the Monod model, the maximum specific degradation rate ( v max ) and half saturation rate constant ( K S ) of the strain were 0.85 h −1 and 101.83 mg·L −1 , respectively. The results of carbon balance revealed that 54.6% carbon was assimilated by R. ruber JJ-3 as biomass, and 43.0% carbon was mineralized into CO 2 . Furthermore, glycerol and lactic acid were measured as intermediates, and the possible degradation pathway was proposed during the biodegradation of acrylic acid. These results suggested that R. ruber JJ-3 completely degrades acrylic acid and might have a potential environmental implication in the purification of acrylic acid-contaminated environments.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Functional Foods
·
2020
·
IF 3.701
Jinglou Chen,Yongfang Lei,Min Liu
Cigarettes consumption is popular all over the world. In the meantime, green tea is widely accepted for their health benefits. Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea. This study aimed to investigate the effects of cigarette smoke (CS) exposure on human prostate cells and rat prostate, as well as the potential protective functions of EGCG. The results suggested that chronic CS exposure stimulated prostatic epithelial-mesenchymal transition (EMT), collagen deposition and fibrosis. Down-regulation of the Hedgehog (HH) signaling pathway helped to ameliorate CS-induced prostatic deficits. Meanwhile, EGCG could exhibit strong anti-fibrosis effects on prostate. Its underlying mechanisms were related to modulate EMT and decrease the activity of HH signal pathway.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）