COLLOIDS AND SURFACES B-BIOINTERFACES
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2026
·
IF 5.9
Weiqiang Wang,Xuan Wang,Dongdong Hu,Xiaoqing Song,Jiaying Qiu,Jiajia Gao,Yutong Liu,Yixuan Tao,Honghua Ge
The extracellular matrix (ECM) is a dynamic, hierarchically structured network essential for mechanical support and regulation of cell behavior. Mimicking its complexity in synthetic materials remains a major challenge. Here, we present a straightforward strategy to fabricate ECM-inspired, dynamically phase-separated hydrogels (DyPS-MAPgel) by methacrylation of mussel adhesive proteins (MAPs), followed by metal ion-induced phase separation and in situ photopolymerization. Leveraging the intrinsic ability of MAPs to undergo biomolecular phase separation and subsequent covalent crosslinking, the resulting hydrogels exhibit tunable mechanical properties, strong tissue adhesion, self-healing, antibacterial, and antioxidant activities. Importantly, DyPS-MAPgels support stem cell proliferation and lineage-specific differentiation both in vitro and in vivo through integrin-dependent mechanotransduction pathways. This work demonstrates the potential of phase separation–mediated assembly as a robust strategy for constructing ECM-mimetic materials and provides insights into ECM-cell interactions. Our findings offer a versatile platform for regenerative medicine applications, including bioadhesion, 3D bioprinting, and tissue engineering.
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NUCLEIC ACIDS RESEARCH
·
2026
·Vol. 54
·期 7
·gkag336
·
IF 15
Haoyun Liu,Ming Zhang,Zhanzhan Xu,Zexin Yang,Yichen Pan,Yongtai Bai,Xinhui Zhao,Weibin Wang,Yang Yang,Jiadong Wang
Emerging evidence suggests a critical interplay between glycolysis and DNA damage repair (DDR) during tumor progression. However, the precise molecular mechanisms coordinating these processes remain unclear. This study reveals a previously unrecognized role of the platelet isoform of phosphofructokinase-1 (PFKP) in homologous recombination (HR) repair. Our investigations demonstrated that ataxia-telangiectasia mutated (ATM) kinase phosphorylates PFKP at residue T278, promoting its transition from tetramers to dimers in response to ionizing radiation (IR) and high glucose (HG) stimulation. The nuclear localized dimeric PFKP recruits casein kinase 2 (CK2), which subsequently phosphorylates RAD51 at residue T13, thereby enhancing the RAD51-BRCA2 interaction and HR efficiency. Clinically, PFKP exhibits significant nuclear accumulation in diabetes-associated malignancies. Importantly, simultaneous targeting of glycolysis and the HR pathway increases tumor sensitivity to radiotherapy. These findings elucidate the pivotal role of PFKP as a metabolic molecular switch that balances glycolysis and DDR, offering new perspectives for the development of metabolically targeted anticancer strategies.
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Advanced Healthcare Materials
·
2026
·
IF 11
Yanzheng Feng,Sikai Di,Zhihao Kang,Xiaodi Lu,Huajin Liu,Xinyan Cong,Tiedong Sun
Single-modal therapies for bacterial infections and tumors suffer from critical bottlenecks, including insufficient reactive oxygen species (ROS) generation, glutathione (GSH)-mediated ROS scavenging, poor targeting, and non-responsive drug release. Herein, a novel biomass-based multifunctional microcomposite (Pt/TiO 2 -D@Lotus) was constructed using natural lotus pollen-derived porous microparticles as the biocompatible matrix, modified with Pt/TiO 2 Janus Schottky heterojunction, and loaded with doxorubicin (DOX). Under near-infrared (NIR) irradiation, the heterojunction efficiently separates photoexcited charges to boost ROS (•O 2 − , •OH, 1 O 2 ) production and depletes 70% of intracellular GSH (500 µg mL −1 ) to amplify oxidative stress. The microcomposite shows a high photothermal conversion efficiency of 55.4% and pH/NIR dual-responsive DOX release (90% release at pH 5.0 + NIR). In vitro experiments demonstrate >99% antibacterial efficiency against S. aureus and E. coli, and ∼85% cancer cell apoptosis rate. In vivo antitumor therapy achieves a 92% tumor inhibition rate with negligible systemic toxicity and good biocompatibility. This work innovatively constructs a biomass-derived synergistic therapeutic platform, providing a feasible strategy to overcome the core limitations of traditional antibacterial and tumor therapies, and expanding the application of biomass materials in biomedicine.
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Horticulturae
·
2026
·Vol. 12
·期 5
·518
·
IF 3.4
Boyin Qiu,Qianrong Zhang,Hui Lin,Jianting Liu,Zuliang Li,Changhui Bai,Qingfang Wen,Dazhong Li,Haisheng Zhu
Fruit flesh thickness is one of the key factors affecting the yield and quality of bitter melon, and its regulatory mechanisms remain unclear. One thick-flesh germplasm (KF) and one thin-flesh germplasm (NF) with significantly different flesh thicknesses were screened from 70 bitter melon germplasms. Through phenotypic surveys, combined metabolomic and transcriptomic analyses, and exogenous sugar treatments, the regulatory mechanisms on flesh thickness were preliminary investigated. The results showed that flesh thickness of the two germplasms remained stable during different years and seasons. Metabolomic and transcriptomic analyses revealed that fructose and mannose metabolism pathway significantly enriched in both omics datasets. The expression of key enzyme encoding genes from this pathway exhibited various expression patterns. In KF, most genes showed significantly higher expression levels than NF, with synergistic expression predominating among genes. Soluble sugar content was positively correlated with gene expression, while HXK, SDH, and TPI activities were negatively correlated with most genes, and FBP activity was positively correlated with most genes. Genes affect carbon source metabolic flux distribution by promoting sugar synthesis and inhibiting sugar respiration consumption. Exogenous sugar treatment exhibited germplasm-specific and concentration-dependent influence of gene expression, with KF primarily showing negative feedback and NF predominantly activating expression. Fruit flesh thickness was significantly positively correlated with the synergistic high expression of sugar metabolism genes and soluble sugar accumulation. This study provides a theoretical basis for molecular improvement of bitter melon fruit flesh thickness.
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Horticulture Research
·
2026
·
IF 9.5
Hu Honglu,Liao Zhenkun,Zhou Chenwen,Liao Bin,Liu Xiaojuan,Zhao Chenning,Wang Dengliang,Liu Lili,Cao Jinping,Wang Yue,Sun Chongde
Polymethoxyflavones (PMFs) are a distinct class of plant flavonoids with significant therapeutic potential, particularly as anti-cancer agents. This potent bioactivity is largely attributable to O-methylation. However, a major bottleneck for the industrial-scale production of PMFs is the lack of suitable precursors and available O-methyltransferases (OMTs), which are essential for constructing a viable biosynthetic pathway. Here, we discover a caffeic acid O-methyltransferase (CsOMT5) whose transcriptional dynamics closely mirrored the PMF levels in the flavedo of sweet orange (Citrus sinensis). In vivo validation further verified that CsOMT5 indeed promoted PMF accumulation in citrus. And its recombinant protein possessed broad substrate promiscuity for various PMF intermediates. Notably, CsOMT5 exhibited specialized C5-regioselectivity for naringenin, a readily available precursor for de novo synthesis of PMFs. Furthermore, critical residues involved this methylation activity (N14, T18, I120, I256, and G305) were revealed through mutational analysis. This study not only provides deeper insights into the PMF biosynthetic pathway but also opens new avenues for the industrial biosynthesis of PMFs.
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Nanoscale
·
2026
·
IF 5.2
Zewen Shen,Tao Jiang,Gehong Liu,Jiayi Liu,Yezi Hu,Haotian Zhang,Huihui Jin,Bingfeng Li,Shuxian Hu,Xiangke Wang,Guixia Zhao,Xiubing Huang
Nuclear energy has emerged as a vital clean energy source with super-high energy density. As a representative of fourth-generation nuclear reactors, High-Temperature Gas-Cooled Reactor (HTGR) generates substantial amounts of tetrahydrofurfuryl alcohol (THFA)-containing wastewater, for which effective treatment methods are rarely explored. Here, we present a selective and efficient approach employing anatase TiO2 as a photocatalyst to upcycle THFA into γ-hydroxybutyric acid (GHB), which can be used as a valuable bifunctional monomer to successful synthesize biodegradable poly(4-hydroxybutyrate). With optimized TiO2 catalyst, a THFA conversion of 87.6% and a GHB yield of 0.198 g/h/m2 were achieved. It was further elucidated that the •OH radicals generated by UV-excited TiO2 initiate the rapid transformation of THFA into GHB through two parallel pathways and the sluggish oxidation of GHB thereby facilitates its accumulation. The developed flow reactor could lead to the total conversion of THFA with GHB as main product that can be purified and separated, demonstrating the practical feasibility of the proposed strategy. This approach showcases the potential for converting THFA-containing wastewater into high-value chemicals through photocatalysis, offering an eco-friendly solution for THFA-containing wastewater treatment.
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Food Chemistry-X
·
2026
·
IF 9.2
Chunlei Tan,Lei Peng,Xiaolin Huang,Mingming Wang,Hongyu Mu,Feng Zhang,Jun Sheng,Yang Tian,Cunchao Zhao
Co-inoculation with Aspergillus oryzae , Lactobacillus plantarum , and Saccharomyces rouxii significantly improved walnut soy sauce (WSS) quality. 72 volatile compounds (VOCs) and 1972 non-VOCs were identified by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) and liquid chromatography–mass spectrometry (LC-MS), respectively. Compared to single A. oryzae fermentation, S. rouxii co-inoculation increased amino acid nitrogen by 36.1% to 1.13 g/100 mL ( p < 0.05), while L. plantarum elevated total acids by 33% and ketones to 54.7% relative content. Triple co-inoculation fermentation achieved the highest umami (7.8 point) and soy sauce-like flavor (8.2 point) with minimal bitterness. Metabolic pathway analysis revealed that key flavor compounds were associated with glycolysis, organic acid, and amino acid metabolism. These findings provide new insights into optimizing the flavor and quality of WSS through mixed-culture fermentation.
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CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Haiyang Liu,Yingjie Shen,Xin Xie,Xudong Mao,Jin Ren,Cunming Peng,Liangliang Wang,Junbo Zhou,Le Zhang,Lulu Zhang,Changchun Peng,Haixia Ge,Shengwen Shao,Jingmou Yu
Wounds complicated by multidrug-resistant (MDR) bacterial infections have posed a severe clinical challenge. The development of innovative and efficient antibacterial materials is of great importance for the management and treatment of MDR-infected wounds. Herein, an injectable sericin/alginate-based hydrogel (CeBG) was constructed by the incorporation of antibacterial compound berberine (BBR)-containing cerium oxide nanozyme. Interestingly, hybrid CeBG exhibited better gelation behavior and mechanical stability compared with the traditional calcium alginate hydrogel. Additionally, CeBG with a highly porous architecture revealed exceptional swelling and adhesive properties. CeBG showed good injectability, biocompatibility, free radical scavenging capacity and effective antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). In the MRSA-infected mouse model, multifunctional CeBG demonstrated the most pronounced wound healing efficacy among all treatment groups. The CeBG-treated group exhibited markedly elevated expression levels of CD31, VEGF, and α-SMA. Notably, CeBG remodeled the inflammatory microenvironment in vivo and influenced macrophage polarization through the regulation of the Toll-like receptor (TLR)/NF-κB signaling pathway, leading to a decrease in pro-inflammatory cytokines like IL-1β, TNF-α and IL-6, while enhancing the production of the anti-inflammatory cytokine IL-10, thus alleviating wound inflammation. Taken together, this composite hydrogel exhibited excellent antibacterial activity and potent antioxidant capacity, offering great potential in the treatment of MRSA-infected wounds.
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Frontiers in Microbiology
·
2026
·
IF 5.8
Abulimiti Abudukadier,Qiao Zhang,Gang Li,Haiqi Chen,Peibo Li,Zhen Gong,Jianping Xie
The MarR family of transcription factors in Mycobacterium tuberculosis plays a critical role in bacterial adaptation to host stresses, yet the function of many members remains unknown. Here, we characterize the novel MarR regulator Rv0737 and its homolog Ms_1492 in M. smegmatis . Overexpression of Rv0737 severely impaired bacterial growth, cell division, and biofilm formation, while increasing susceptibility to oxidative stress and the cell wall-targeting drug isoniazid. Conversely, deletion of ms_1492 altered cell envelope permeability and lipid composition, enhanced ATP synthesis, and conferred mild tolerance to H 2 O 2 and isoniazid. Lipid profiling and transcriptomic analysis revealed significant dysregulation of lipid metabolism genes. Crucially, electrophoretic mobility shift assays demonstrated that both Rv0737 and Ms_1492 directly bind to the promoter region of the sigL-rslA operon, which encodes an alternative sigma factor and its anti-sigma factor. Our findings establish a direct regulatory pathway wherein Rv0737/Ms_1492 modulates bacterial growth, cell envelope integrity, and stress response by targeting the sigL-rslA operon, identifying this system as a potential therapeutic target for combating drug-resistant tuberculosis.
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Frontiers in Nutrition
·
2026
·
IF 5.5
Disha Jiang,Yulin Wang,Yun Ling,Sergei A Eremin,Liliya I Mukhametova,Jinglin Du,Hao Hu
This study investigated the dynamic changes in rice quality, microbial communities, and volatile compound profiles during simulated summer transportation (35 °C, 70% RH, 15 days). Indica rice samples were systematically collected every 3 days and analyzed using HS-SPME-GC-MS/MS, HS-GC-IMS, and metagenomic sequencing. Prolonged transportation significantly altered the physicochemical properties of the rice. Moisture content plateaued on day 12, while germination rates declined significantly starting from day 6. Furthermore, fatty acid values increased continuously due to accelerated lipid hydrolysis and oxidation. Visible mold growth became evident on day 12, marking a critical tipping point for quality deterioration. The odor activity value (OAV) and relative odor activity value (ROAV) analyses revealed that the decline in unsaturated fatty aldehydes such as (E)-2-nonenal and the significant accumulation of alcohols, ketones, and short-chain esters, including 1-octen-3-ol and ethyl acetate, drove the transition from a “fresh and fatty” aroma to one characterized by moldy, fermented, and pungent notes. Metagenomic analysis demonstrated a profound ecosystem shift from bacterial dominance (Proteobacteria, Actinobacteria) to fungal dominance. Notably, Lichtheimia surged from <0.01% to 23.95%, becoming the dominant genus, while Aspergillus increased from 0.03% to 4.57%. Correlation analysis indicated that while Pseudomonas was associated with elevated fatty acid levels, the flavor shift was primarily linked to microbial succession. These findings provide insights into the synergistic mechanisms of rice spoilage and suggest that specific volatile markers could serve as early warning indicators for quality control in real-world grain logistics.
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Biomedicines
·
2026
·Vol. 14
·期 3
·661
·
IF 4.5
Luojia Jiang,Ying Li,Fang Wan,Yi Su,Meixian Lei,Yupei Li,Haibo Xu
Background/Objectives:The strategy of targeting endotoxins and circulating histones to alleviate excessive inflammation and tissue damage has been proposed as an important immunoregulatory strategy against sepsis. However, the development of a multifunctional hemoperfusion adsorber that simultaneously removes endotoxins and histones remains an unmet clinical need in sepsis management.Methods: We synthesized chitosan-cellulose composite (CSCE) microspheres utilizing phase inversion technology, while heparin-grafted chitosan-cellulose composite (CSCEHEP) microspheres were developed by grafting heparin onto CSCE microspheres through the carbodiimide coupling method. In our experimental design, we allocated healthy New Zealand rabbits to four distinct groups: a healthy control group, a lipopolysaccharides (LPS) group, a CSCE group, and a CSCEHEP group. Following the administration of LPS for 12 h, septic rabbits underwent extracorporeal hemoperfusion with either CSCE or CSCEHEP microspheres for a duration of 6 h, notably without the inclusion of heparin in the blood circuits. Post-hemoperfusion, we conducted an analysis of thrombus formation and total protein adsorption on the column. Concurrently, blood samples were collected from the venous side to evaluate inflammatory cytokine concentrations, liver and kidney function levels, LPS levels, the histone presence, and to perform histopathological assessments of liver and kidney injury.Results: Our in vivo experiments demonstrated that CSCEHEP microspheres for extracorporeal circulation could achieve a 6 h hemoperfusion session in septic rabbits without the need for continuous anticoagulation with heparin. A CSCEHEP column turns into a very light-red color (almost the original white) and light contamination or clotting was observed after the 6 h hemoperfusion. Moreover, CSCEHEP microspheres effectively reduced the concentration levels of leukocyte, serum IL-6 and TNF-α, mitigated pathological damage to the liver and kidneys, and removed over 56.7% of LPS and nearly 58.6% of histone H3 from the blood of septic rabbits during hemoperfusion.Conclusions:Hemoperfusion utilizing CSCEHEP microspheres exhibits excellent self-anticoagulation capabilities, remarkable anti-inflammatory performance, efficient endotoxin adsorption and histone antagonism properties, rendering it both effective and safe for use in septic rabbits.
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AICHE JOURNAL
·
2026
·
IF 4.4
Haolin Wu,Hongbin Liu,Bin Wang,Fan Wang,Amr F. M. Ibrahim,Miao Yu,Shenglai Zhong,Weihong Xing,Rongfei Zhou
The industrial application of microporous crystalline membranes for gas separation has been persistently hindered by the inefficiency of conventional batch synthesis. To address this limitation, we reported a continuous and ultrafast synthesis (CUS) strategy that enables the efficient production of ultrathin b -oriented MFI and SSZ-13 zeolite membranes in just 10–15 min. The resulting MFI and SSZ-13 membranes exhibited superior separation performance in n -butane/ i -butane and CO 2 /N 2 mixtures, respectively, far exceeding those of previously reported membranes. Remarkably, the CUS process achieved a two-order-of-magnitude reduction in synthesis time, a 95% decrease in energy consumption and a 98% reduction in gel consumption compared to the batch process. Process simulation using Aspen Plus confirmed that energy consumption was reduced by over 87.6% with membrane separation compared to conventional distillation for butane isomer separation. This work establishes an efficient, scalable, and economically viable pathway for industrial-scale fabrication of zeolite membranes for gas separation.
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