BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Yan Wang,Boya Xu,Zehan Zeng,Haixin Wang,Lin Shi,Wanting Liu,Yanyan Chen,Xuexian Deng,Jun Chen,Jin-Xiang Chen
Extracellular vesicle (EV)-based liquid biopsy holds great promise for glioma diagnosis, but its clinical translation remains hindered by inefficient isolation of disease-relevant EV subpopulations and insufficiently integrated signal validation. Herein, we develop a platform that integrates a bifunctional nanozyme system (phosphatase-like MOF and peroxidase-like Pt) with a CHA-CRISPR/Cas12a cascade, achieving selective isolation and ultrasensitive detection of EGFR-positive glioma-derived EVs. Defective UiO-66-NH 2 loaded with Pt nanoparticles and functionalized with EGFR aptamers (UiO@Pt@Apt) is immobilized on Hook strand-modified glass 96-well plates via Apt-Hook hybridization. Upon introduction of EGFR-positive EVs, they specifically bind to aptamers on UiO@Pt@Apt and induce the release of UiO@Pt@Apt-EV complexes through perturbation and destabilization of the Apt-Hook interface. After EV lysis, EV-derived miRNA-21 activates the CHA-CRISPR/Cas12a cascade to generate a fluorescent signal, while the nucleotide fragments produced by Cas12a trans-cleavage are proposed to be hydrolyzed by phosphatase-like defective UiO-66-NH 2 to generate PO 4 3− . The PO 4 3− may contribute to Pt-mediated TMB oxidation, thereby supporting colorimetric signal amplification. The platform achieved a colorimetric EV detection limit of 427 particles/μL after coupling with the CHA-CRISPR system, representing a 6.9-fold improvement over the UiO@Pt@Apt system alone, and enabled a miRNA-21 detection limit of 87.0 fM. In plasma samples from 25 glioma patients and 20 healthy donors, the combined readout achieved an AUC of 0.968, showing numerically better discriminatory performance than either single readout, although the improvement was not statistically significant by DeLong analysis. This work provides a promising strategy for EV-based glioma liquid biopsy and offers a potentially adaptable framework for cascade-coupled dual-signal biosensing.
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高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2021
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IF 19.503
Ziyang Chen,Long Liang,Huan Yuan,Huan Liu,Peng Wu,Mingli Fu,Junliang Wu,Peirong Chen,Yongcai Qiu,Daiqi Ye,Limin Chen
Strong metal-support interaction (SMSI) is regarded as one of the most important concepts in heterogeneous catalysis; however, few researches are focused on SMSI regulating the support structure, properties and their roles on the catalytic performance. Herein, anatase TiO 2 {001} nanosheets pretreated in different atmosphere were used to load Pt for reverse water gas shift reaction. The Pt/N-doped TiO 2 catalysts exhibited significantly superior catalytic performance. The investigation demonstrated that defects and doped-N enhance the Pt dispersion while Pt and SMSI in turn promote defects regeneration during activation in H 2 . During reaction, surface defects enable CO 2 activation to generate active intermediates which easily decompose into CO. Furthermore, the small Pt particles and SMSI facilitate H 2 dissociative adsorption and spillover to replenish both surface defects and bulk Ti 3+ . Besides, a novel reaction route has been observed that the stable bulk Ti 3+ in N-doped TiO 2 migrate and transform to surface defects, enhancing catalytic performance.
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ACS Applied Materials & Interfaces
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2026
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IF 7.8
Wenxuan Lu,Simin Wang,Yuxuan Xu,Xiaoxia Wang,Xijun Wang,Kun Zhao,Yunfei Gao,Fuchen Wang
The integration of an intermetallic Pt alloy and single-atom M–N–C support is an intriguing strategy to combine the advantages of both counterparts and further lower Pt usage at higher power output. Despite prior attempts, the fuel cell performance is still unsatisfactory, and the atomic diffusion/alloying pathway between the Pt alloy and M–N–C is still not clear. Here, we control the Co amount in the CoNC and present the atomic-diffusion pathway for the in situ formation of Pt-based intermetallic catalysts with size-controlled nanoparticles (NPs). The synthesized Pt3Fe–Co@5CoNC demonstrates excellent performance in half-cell ORR tests. Under H2–air conditions, the membrane electrode assembly exhibits low Pt usage (0.175 g kW–1), good stability with a 12.5 mV loss at 0.8 A cm–2 at the end of the test, and an ultrahigh power density of 1.717 W cm–2 at 0.429 V. Comprehensive experimental results and DFT calculations show the atomic pathway of diffusion and alloying for low-coordinated Co–N3–C into L12-Pt3Fe NPs. In comparison, high-coordinated Co–N4–C remains stable and contributes to the stability of the intermetallic Pt alloy through the strong Pt–Co–N4 interaction. This work demonstrates important progress in the integration of intermetallic Pt alloy and single-atom CoNC for ORR and can be extended to other single-atom supports, including Ni-NC, Fe-NC, and Cu-NC.
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高影响因子
ACS Applied Materials & Interfaces
·
2022
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IF 10.383
Nan Wang,Xiangjie Bo,Ming Zhou
The development of cost-effective Pt-based electrocatalysts is of great scientific and industrial significance for improving the electrocatalytic activity of hydrogen evolution (HER) and oxygen evolution (OER) reactions for overall water splitting. In this work, unlike traditional furnace pyrolysis, we report the rapid and single-step room-temperature synthesis of Pt/VC nanocomposites with a three-dimensional (3D) network porous structure by laser irradiation technology. The resultant Pt-based composite (Pt/VC-2.84) could be applied to HER under different pH conditions. In particular, the content of Pt in Pt/VC-2.84 is only 2.84 wt %, which is far lower than that in the advanced HER electrocatalyst with the Pt content of 20 wt % (commercial 20 wt % Pt/C). In addition, Pt/VC-2.84 exhibits a boosted higher OER activity and stability than RuO2 in an alkaline medium. Most importantly, electrocatalytic results reflect that Pt/VC-2.84 reveals superior activity and stability toward overall water splitting.
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高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 97
·期 8
·2055-2067
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IF 3.709
Xiaolong Liu,Jingye Ma,Minxin Wang,Hong Yuan
BACKGROUND A series of mesoporous Pt@KIT−6/SAPO−11 catalysts with SiO 2 :Al 2 O 3 molar ratios of 0.3, 0.4, or 0.5 and Pt loadings of 0.4, 0.8, or 1.2 wt% were prepared via an in situ encapsulation process using a PEO − PPO − PEO triblock copolymer (EO 20 PO 70 EO 20 , P123) as the mes. China 750001 oporous template and KIT−6 molecular sieves as the silicon source. RESULTS These catalysts were characterized by transmission electron microscopy (TEM), specific surface area analysis, and pyridine–infrared spectroscopy (Py − IR). The results showed that Pt nanoparticles with particle sizes of 3–7 nm were uniformly dispersed on the supports. These materials also exhibited good mesoporous structures with specific surface areas of 138–197 m 2 /g and average pore sizes of 7.1–9.9 nm. The specimens having a SiO 2 :Al 2 O 3 molar ratio of 0.4 also showed an orderly strip−shaped pore structure. The Py − IR results indicated that the catalysts had both Brønsted acid and Lewis acid sites, with total acid contents in the range of 77.8–131.1 μmol/g. CONCLUSION The decarboxylation of oleic acid using these materials under CO 2 was examined. The catalyst with a SiO 2 :Al 2 O 3 molar ratio of 0.4 and a Pt loading of 0.8 exhibited the best catalytic performance and a C8 − C17 alkanes yield as high as 85.1% at 340 °C by using a catalyst−to−oil mass ratio of 1:10 and a reaction time of 5 h. This performance was attributed to the ordered mesoporous structure of this catalyst along with its high surface area (173 m 2 /g), good crystallinity (92.8%), suitable acid content (98.6 μmol/g), and abundant Pt metal active sites. © 2022 Society of Chemical Industry (SCI).
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ACS Agricultural Science & Technology
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2022
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IF 0
Can Zhang,Jinnuo Hu,Xiaoxiao Wu,Jiyong Shi,Bruce D Hammock
As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. A gold–platinum (Au@Pt) nanozyme is a kind of mimic enzyme with unique properties of nanomaterials and catalytic activity. The anti-3-PBA nanobodies were labeled with Au@Pt (Au@Pt-Nbs) by electrostatic adsorption. The Au@Pt-Nbs lateral-flow nanozyme immunoassay was established for the visual and rapid detection of 3-PBA. The visualization results were quantitatively analyzed by Adobe Photoshop CC and got a cutoff value of 0.005 ng/mL. The samples of milk- and lake water-spiked 3-PBA were also analyzed, and the recoveries ranged from 102 to 108% and 95 to 112%, respectively. The results were also validated by liquid chromatography–mass spectrometry and got good correlation (R2 = 0.945). The Au@Pt-Nbs lateral-flow nanozyme immunoassay was developed by the combination of nanobodies and nanozymes, which will be a promising application in the field of safety inspection.
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Advanced Materials Interfaces
·
2021
·Vol. 8
·期 24
·2101543
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IF 6.147
Wei Zhang,Tao Liu,Yuansheng Shan,Bailing Hou,Zhenyu Chu,Xiaoping Gu,Zhengliang Ma,Wanqin Jin
Due to the short course and high mortality of acute myocardial infarction (AMI) which will cause irreversible myocardial necrosis to induce cardiac arrest, its early diagnosis within minutes is always a challenge in clinical emergency. In this work, an ultrafast and ultrasensitive aptasensor is designed to achieve quantitatively detection of cardiac troponin I (cTnI) which is proved as a highly specific and sensitive biomarker to AMI, and within only 4 min, the cTnI concentration can be accurately reported. This aptasensor is constructed via an in situ growth of the oriented Pt-polyaniline (PANI) needle-like nanoarrays providing both high electrocatalysis and abundant active sites as the signal magnifier and DNA carrier. A target-induced strand release strategy is proposed to remarkably accelerate the recognition process of cTnI through the specific design of DNA stand for binding. The as-prepared aptasensor can exhibit an ultrawide linear detection range of cTnI from 1 pg mL −1 to 100 ng mL −1 satisfying the clinical demand for AMI diagnosis, together with excellent selectivity among various biomarkers. Based on its fast recognition ability, this aptasensor presents a high accuracy in the practical detection of human serum to prove its promising ability in the early and on-site diagnosis of AMI.
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高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
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2021
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IF 3.101
Xiaofeng Wang,Yongfeng Chen,Runze Yu,Ruiqiang Wang,Zhixiang Xu
In this paper, a sensitive biomimetic enzyme-linked immunoassay (BELISA) method based on an Au@Pt@Au composite nanozyme label was developed for histamine (HA) detection. A molecular imprinted polymer prepared directly on the 96-well plate served as a biomimetic antibody. A BELISA method was developed for HA standard solutions at concentrations ranging from 0.01 to 100.00 mg/L. Under optimal conditions, the novel BELISA method had a low limit of detection (0.069 mg/L) and high sensitivity (7.20 mg/L). The HA spiked in yellow rice wine and liqueur samples were detected by this BELISA method with the recoveries ranging from 84.28% to 108.82%. There was no significant difference of the results between the BELISA method and HPLC for the detection of HA in the red and white grape wine samples.
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Molecular Catalysis
·
2021
·
IF 5.062
Guoliang Gao,Huangze Yu,Xue Lu Wang,Ye-Feng Yao
The alloy catalyst formed by transition metal and a small amount of noble metal has become the most promising substitute for M-based ( M = Pt, Pd, Ru, Rh) catalyst. However, due to the direct exposure of the metal core to the electrolyte, it is vulnerable to corrosion and oxidation, which in turn reduces the catalytic stability and is becoming a major obstacle to sustainable hydrogen production. The present work addresses this issue by developing a one-step immersion-adsorption-pyrolysis strategy for synthesizing FeM alloy nanoparticles with N-doped carbon coatings (FeM@CN) for use as electrocatalysts in the HER. The deliberately designed metal-organic framework material was used as the precursor of catalyst synthesis to achieve the carbon coatings and simultaneously the heteroatom in-situ doping for the alloy nanoparticles. The optimal FePt@CN demonstrates excellent catalytic stability and HER activity in an acidic electrolyte medium. The reactions obtain a small overpotential of 28 mV to achieve current densities of 10 mA cm −2 , which are comparable to a high-performance commercial Pt/C electrocatalyst with a much higher Pt loading. The FeRu@CN also demonstrates an outstanding performance with an overpotential of only 18 mV to achieve a current density of 10 mA cm −2 in an alkaline medium.
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CHEMICAL ENGINEERING JOURNAL
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2020
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IF 10.652
He Xiao,Shoufeng Xue,Jingjuan Zhang,Man Zhao,Jianchun Ma,Shuai Chen,Zhanfeng Zheng,Jianfeng Jia,Haishun Wu
Designing high-performance Pt-based catalysts with low catalysts cost is still a great challenge. Here, we demonstrate a facile electrolysis-solvothermal technique for making Pt and carbon quantum dots coloaded multiwall carbon nanotube (Pt-CQDs/MWCNT) catalyst with the assistant of electric field. Despite of the ultralow Pt amount (0.81 wt%) for the optimized Pt-CQDs/MWCNT catalyst, it exhibits ultrahigh electroactivity and durability toward hydrogen evolution reaction (HER) and ethanol oxidation reaction (EOR). The mass activities of the catalyst for HER and EOR can be achieved to 4889.7 mA/mg (-40 mV) and 4917 mA mg −1 Pt (forward peak), which are 8.1 times and 5.6 times more active than those of commercial Pt/C respectively. The characterization results reveal that Pt nanpparticles (Pt NPs) with intrinsic structure play the key role in excellent catalytic performance for HER and EOR. CQDs not only support abundant anchoring points for fixation and dispersion of Pt NPs, but also construct 3D conductive network with MWCNT supporter for improving the catalytic performance.
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高影响因子
APPLIED CATALYSIS A-GENERAL
·
2020
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IF 5.006
Yankun Du,Jianwu Zou,Yang Guo,Xiang Xu,Hongyu Chen,Changqing Su,Zheng Zeng,Liqing Li
The low-Pt bimetal compounds (PtNi 3 -C and PtFe 3 -C) were synthesized via the chemical reduction method and the toluene catalytic oxidation experiments were conducted to test their catalytic performance. The sample of PtFe 3 -C (T 100 = 255 ℃) possessed a better low-temperature catalytic activity than PtNi 3 -C (T 100 = 288 ℃) due to a higher content of Pt (0), while both two catalysts had a desirable catalytic stability (⁓100 % within 72 h). The reaction kinetic parameters were acquired from the Mars-van Krevelen (MVK) model, and the MVK mechanism combined with the in-situ DRIFT was used to investigate the reaction pathway. Moreover, the theoretical calculations based on DFT was applied to study the accessibility of toluene to the surface of metal atoms doping. Herein, this study not only can provide a promising catalyst with the superior low-temperature catalytic activity and stability, but also can give a comprehensive understanding on the toluene catalytic oxidation mechanism over bimetal catalysts.
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高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2020
·Vol. 95
·期 6
·1756-1772
·
IF 2.75
Guanshu Li,Yuejiao Cao,Ludong Yi,Jun Wang,Youtao Song
BACKGROUND The recovery and reuse of sonocatalysts are the pivotal issues in the sonocatalytic degradation of organic pollutants. The use of some natural minerals as the carrier is not only beneficial to recycling of sonocatalysts, but also reduces the treatment costs of organic pollutants. Two kinds of hydroxyapatite (HAP), Ox Bone (OB)-HAP and synthesized (Syn)-HAP combine with the Pt-TiO 2 /(Er 3+ :Y 3 Al 5 O 12 @Ta 2 O 5 ) (P-TET) composite, obtaining two Z-scheme sonocatalysts, P-TET/OB-HAP and P-TET/Syn-HAP, respectively. The sonocatalytic activities of the prepared samples are investigated via degradation of prometryn and disinfection of Escherichia coli . RESULTS A series of characterizations showed that the Z-scheme P-TET/(OB or Syn)-HAP sonocatalysts were prepared successfully. To evaluate their sonocatalytic activities, some influencing factors such as ultrasonic irradiation time, inorganic oxidants, used times and trapping agents on the sonocatalytic degradation prometryn were determined. The best degradation ratio (80.31% based on N computing and 85.07% based on S atom computing) of prometryn could be obtained for 10 mmol L −1 K 2 S 2 O 8 , 1.0 g L −1 P-TET/OB-HAP sonocatalyst and 150 min ultrasonic irradiation. The excellent sonocatalytic disinfection ratio (88.65%) of E. coli could also be obtained under 40 min ultrasound irradiation. Moreover, a possible mechanism on sonocatalytic degradation of prometryn was illustrated. CONCLUSION The treated bovine bone HAP was directly used as a carrier to combine with P-TET to construct a novel Z-scheme P-TET/OB-HAP sonocatalyst. The high degradation ratio of prometryn and disinfection ratio of E. coli showed that P-TET/OB-HAP could be a promising sonocatalyst for the degradation of organic pollutants and the disinfection of bacteria. © 2020 Society of Chemical Industry
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