SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Mingxin Li,Hong Yang,Shuqun Li,Chunwei Liu,Kang Zhao,Jianguo Li,Danni Jiang,Lulu Sun,Hong Wang,Anping Deng
3-Amino-5-methylmorpholino-2-oxazolidinone (AMOZ) is the metabolite of furaltadone (FTD), one kind of nitrofuran antibiotics which have been banned as veterinary drugs due to the potential harmful effects on human health. AMOZ is stable in tissue matrices, and can be used as an indicator for illegal usage of FTD. In this study, a novel ultrasensitive competitive immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for direct detection of AMOZ in tissue and urine samples was developed. Monoclonal antibody (mAb) against AMOZ and Raman reporter 4-mercaptobenzoic acid (MBA) dual labelled colloidal GNPs ( e.g. Ab-Au-MBA) was prepared, characterized and used as an immunoprobe. The specific Raman scattering intensity of MBA on the test line of the ICA strip was measured for quantitative determination of AMOZ. The assay was completed within 15 min. The values of IC 50 and limit of detection (LOD) of the assay for AMOZ were 0.04 ng mL −1 and 0.28 pg mL −1 , respectively. There was no cross-reactivity (CR) of the assay with other three nitrofurans and five compounds. The ICA strips could be used for 7 weeks without loss of activity. The tissue and urine samples were spiked with AMOZ and the recoveries of AMOZ were in range of 87.8–105.9% with the relative standard deviations (RSD) of 1.4–9.0% ( n = 3). The results demonstrated that the proposed SERS-based ICA was able to directly detect AMOZ with high sensitivity, specificity, stability, precision and accuracy, and might be as a powerful method for the analysis of other analytes in different matrices.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Xin Jiang,Kaiwei Li,Yimin Tang,Xiuwen Wang,Wei Kan,Libin Yang,Bing Zhao
High-performance flexible semiconductor material can be used as an excellent multifunctional matrix for in-situ ultrasensitive surface-enhanced Raman scattering (SERS) detection and synchronous photocatalytic degradation of antibiotic residues in aquatic ecosystem. Here, a calcium-doped TiO 2 flexible matrix with double defects (surface oxygen vacancy defect and Ti 3+ energy level defect) was developed by its “in-situ one-step” hydrothermal synthesis on cotton fabric for the above purposes. Due to the joint contribution of double defects, a multi-channel charge transfer mode and a high-efficiency carrier separation are achieved, which endows flexible cotton fabric/Ca-doped TiO 2 (Cot/Ca-TiO 2 ) substrate with the greatly boosted SERS effect for in-situ detection of antibiotic residues on fish body surface and in fishpond water by a simple wiping or dipping sampling method, even for simultaneous identification of multi-component residues. The detection limits of three antibiotic residues (enrofloxacin, ciprofloxacin and enoxacin) are as low as 10 −9 M, which are far lower than the EU standard. More meaningfully, the flexible Cot/Ca-TiO 2 can be used as a multifunctional filter-membrane type photocatalyst for efficient on-site degradation of antibiotic residues in flowing fishpond water by a multi-grade photocatalysis means. Moreover, the flexible matrix exhibits good recyclability in both actual detection and photocatalysis.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Shi Xuerong,Wang Wanru,Xie Xinyu,Gong Changtian,Zhang Wenyuan,Zhu Xinyue,Liu Xiaoyan,Zhang Haixia
Pigments in painted cultural relics serve as critical historical evidence, offering significant research value for understanding historical contexts and cultural development processes. However, the pigments, especially organic pigments (or dyes), have progressively degraded over time, resulting in severe fading that challenges their sensitive identification. To address this issue, we developed an innovative sampling methodology combined with surface-enhanced Raman spectroscopy (SERS) analysis for identifying indigo pigment in painted cultural relics. The methodology employs calcium alginate gel beads doped with either gold nanobipyramid (Au NBPs/CAGBs) or hybrid Au–Ag nanoprisms (Ag NPrs/Au NBPs/CAGBs), enabling minimally invasive sampling of indigo through controlled interactions with the mural surface. Subsequent SERS analysis of the retrieved substrates demonstrated enhanced molecular identification capabilities compared to conventional in situ non-destructive methods. Furthermore, microscopic examination and color difference quantification (Δ E < 1.5) revealed no observable surface alterations on tested mural samples before and after sampling, which confirmed the method’s minimal invasiveness. Finally, the approach was successfully applied to imitation heritage objects, including Tang Tri-color (“Lady Spring Festival”) replica ceramics and bottle-type simulated ceramic artifacts before and after aging, achieving effective sampling and pigment identification. This study establishes a novel paradigm for sensitive pigment analysis in cultural heritage conservation, with promising implications for future relic restoration and protection strategies. Graphical
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Long Ma,Wenlu Zhang,Lijuan Yin,Yaru Li,Jianwen Zhuang,Liang Shen,Shuli Man
The pandemic of COVID-19 creates an imperative need for sensitive and portable detection of SARS-CoV-2. We devised a SERS-read, CRISPR/Cas-powered nanobioassay, termed as OVER-SARS-CoV-2 (One-Vessel Enhanced RNA test on SARS-CoV-2), which enabled supersensitive, ultrafast, accurate and portable detection of SARS-CoV-2 in a single vessel in an amplification-free and anti-interference manner. The SERS nanoprobes were constructed by conjugating gold nanoparticles with Raman reporting molecular and single-stranded DNA (ssDNA) probes, whose aggregation-to-dispersion changes can be finely tuned by target-activated Cas12a though trans -cleavage of linker ssDNA. As such, the nucleic acid signals could be dexterously converted and amplified to SERS signals. By customizing an ingenious vessel, the steps of RNA reverse transcription, Cas12a trans -cleavage and SERS nanoprobes crosslinking can be integrated into a single and disposal vessel. It was proved that our proposed nanobioassay was able to detect SARS-CoV-2 as low as 200 copies/mL without any pre-amplification within 45 min. In addition, the proposed nanobioassay was confirmed by clinical swab samples and challenged for SARS-CoV-2 detection in simulated complex environmental and food samples. This work enriches the arsenal of CRISPR-based diagnostics (CRISPR-Dx) and provides a novel and robust platform for SARS-CoV-2 decentralized detection, which can be put into practice in the near future.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Changfei Ma,Qun Zhang,Jing Liang,Shan Yang,Tianlong Zhang,Fangqi Ruan,Hongsheng Tang,Hua Li
Polycyclic aromatic hydrocarbons (PAHs) present in oily sludge generated by the petroleum and petrochemical industries have emerged as a prominent concern within the realm of environmental conservation. The precise determination of PAHs holds immense significance in both petroleum geochemistry and environmental protection. In this study, a combination of surface-enhanced Raman spectroscopy (SERS) and solid–liquid extraction was employed for the screening of PAHs in oily sludge. Methanol was utilized as the extraction solvent for PAHs, while nanosilver-silicon coupling substrates were employed for their detection. The SERS spectrum was acquired using a portable Raman spectrometer. The nano silver-silicon coupling substrate exhibits excellent uniformity, with relative standard deviations (RSDs) of Phenanthrene, Fluoranthrene, Fluorene and Naphthalene (Phe, Flt, Flu and Nap) being 2.8%, 1.08%, 1.41%, and 5.44% respectively. Moreover, the limits of detection (LODs) achieved remarkable values of 0.542 μg/g, 0.342 μg/g, 0.541 μg/g, and 5.132 μg/g. The quantitative analysis of PAHs in oily sludge was investigated using SERS technology combined with partial least squares (PLS). The optimal PLS calibration model was optimized by combining spectral preprocessing methods and using the SiPLS (Synergy interval partial least squares)-VIP (Variable Importance in Projection) hybrid variable selection strategy. The prediction performance of the D1st (First derivative)-WT (Wavelet transform)-SiPLS-VIP-PLS model was deemed satisfactory, as evidenced by high R 2 P values of 0.9851, 0.9917, and 0.9925 for Phe, Flt, and Flu respectively; additionally, the corresponding MREP values were found to be 0.0580, 0.0668, and 0.0669 respectively. However, for Nap analysis, the D1st-WT-PLS model proved to be a better calibration model with an R 2 P value of 0.9864 and an MREP (Mean relative error of prediction) value of 0.0713. In summary, SERS technology combined with PLS based on different spectral pretreatment methods and mixed variable selection strategies is a promising method for quantitative analysis of PAHs in oily sludge, which will provide new ideas and methods for the quantitative analysis of PAHs in oily sludge.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Qinzhi Wang,Zhihao Xu,Yijian Zhao,Hui Zhangsun,Tong Bu,Changqiu Zhang,Xin Wang,Li Wang
Surface-enhanced Raman scattering (SERS) enables azo dye monitoring become rapid and efficient. However, the high cost, low efficiency and poor recyclability of SERS substrate limit its practical application. Herein, we propose a facile strategy to develop self-cleaning carbon cloth as a high-performance and reusable SERS substrate through in-situ growth of leaf-like metal-organic framework (L-MOF) and one-step electro-deposition of flower-like Ag nanoparticles (CC-L-MOF@F-Ag) followed by chemical modification for SERS detection of congo red (CR), chrysoidine (CYD) and acid orange II (AO II) in soft drinks. Taking advantages of superhydrophobicity and SERS-active F-Ag, the prepared self-cleaning CC-L-MOF@F-Ag not only avoids the diffusion-random problem and further boosts sensitivity, but also yields excellent self-cleaning property through facile washing with water, endowing its good reusability and stability. Furthermore, this substrate can realize quantitative detection of CR, CYD and AO II with low limits of detection (0.013 μM for CR, 0.017 μM for CYD and 0.0067 μM for AO II) and wide linear detection range (0.1 – 1000 μM for CR, 0.1 – 1000 μM for CYD and 0.01 – 100 μM for AO II). Meanwhile, CR, CYD and AO II in six soft drinks were also successfully detected with satisfactory recoveries. This method provides a potential and cost-effective strategy to develop advanced SERS sensing device for effective monitoring azo dyes in soft drinks.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Meng Zhang,Yanan Wang,Yan Ma,Xu Wang,Bing Zhao,Weidong Ruan
Surface-enhanced Raman scattering (SERS) has been applied in many fields, but still has the limitation of widespread applications on semiconductor substrates. In this work, a series of antimony-doped tin oxide (ATO) nanoparticles (NPs) have been synthesized by a hydrothermal method and were used as SERS substrates for the first time. Interestingly, a charge transfer (CT) effect was revealed between the probing molecules of 4-mercaptobenzoic acid (4-MBA) and the substrates of ATO NPs, which accounts for the SERS enhancement and shows dependence to the Sb ions doping ratios in ATO NPs. By considering the energy level diagram of the ATO–MBA complexes and the doping theory of semiconductors, this phenomenon is believed to connect to the variance of the optical band gap energy ( E g ), which is accompanied with the changes of free charge carrier densities in conduction bands (CBs) of ATO NPs due to different doping contents. The study of the E g - or free-charge-carrier-density-dependent property of the semiconductor-based SERS provides a new point of view for the development of new semiconductor SERS substrates and also contributes to the SERS CT mechanism.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-10
·
IF 5.3
Qu Xiangwen,Zhou Pengwei,Shi Boya,Zheng Yekai,Kan Lian,Jiang Li
A multifunctional surface-enhanced Raman scattering (SERS) platform integrating sensitive detection and drug resistance analysis was developed for Gram-positive bacteria. The substrate was based on self-assembled Ti 3 C 2 T x @Au NPs films and capture molecule phytic acid (IP6) to achieve specific capture of Gram-positive bacteria and different bacteria were analyzed by fingerprint signal. It had advantages of good stability and homogeneity (RSD = 8.88%). The detection limit (LOD) was 10 2 CFU/mL for Staphylococcus aureus and 10 3 CFU/mL for MRSA, respectively. A sandwich structure was formed on the capture substrate by signal labels prepared by antibiotics (penicillin G and vancomycin) and non-interference SERS probe molecules (4-mercaptobenzonitrile (2223 cm −1 ) and 2-amino-4-cyanopyridine (2240 cm −1 )) to improve sensitivity. The LOD of Au NPs@4-MBN@PG to S. aureus and Au NPs@AMCP@Van to MRSA and S. aureus were all improved to 10 CFU/mL, with a wide dynamic linear range from 10 8 to 10 CFU/mL ( R 2 ≥ 0.992). The SERS platform can analyze the drug resistance of drug-resistant bacteria. Au NPs@4-MBN@PG was added to the substrate and captured MRSA to compare the SERS spectra of 4-MBN. The intensity inhomogeneity of 4-MBN at the same concentrations of MRSA and the nonlinearity at the different concentrations of MRSA revealed that MRSA was resistant to PG. Finally, the SERS platform achieved the determination of MRSA in blood. Therefore, this SERS platform has great significance for the determination and analysis of Gram-positive bacteria. Graphical Abstract
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Dongxue Yu,Yimin Tang,Ziqi Liu,Lihui Hu,Xin Jiang,Bing Zhao,Libin Yang
Developing new-type semiconductor surface-enhanced Raman scattering (SERS) platform with synchronous enhancement of sensitivity and stability is an effective strategy for achieving ultra-sensitive detection of emerging pollutants in various pollution scenarios. In this work, a new g-C 3 N 4-x /N-doped TiO 2-x (g-C 3 N 4-x /N-TiO 2-x ) heterojunction SERS matrix was purposefully developed by a combined strategy of interface engineering and defect engineering. During the formation of heterojunction, “N-bidirectional migration” between two monomers can be achieved (that is, N vacancies and N doping can be generated simultaneously), which achieves a synchronous modulation of the atomic and electronic structures of the heterojunction. This endows g-C 3 N 4-x /N-TiO 2-x with excellent interfacial performance, abundant defect states, as well as ultra-high stability. The enhancement factor of the SERS matrix is as high as 10 9 . Theoretical calculations and experiments reveal the contribution of charge transfer (CT) mechanism to the SERS enhancement. The high-efficiency carrier separation induced by the strong interface coupling effect and the multi-channel CTs mediated by multiple defect states (vacancy defects and doping defects) are jointly responsible for the observed huge SERS enhancement. Benefiting from its high SERS sensitivity (selectivity) and excellent stability, g-C 3 N 4-x /N-TiO 2-x can be applied for ultrasensitive detection of antibiotic pollutants in multiple scenarios, including animal-derived foods such as milk, sour milk and beef, as well as natural water systems such as lake water and seawater. The detection limit of several typical antibiotic residues (enoxacin, ciprofloxacin, sulfamethoxazole and florfenicol) is as low as 1 × 10 −11 mol/L.
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Journal of Materials Chemistry C
·
2023
·Vol. 11
·期 43
·15138-15148
·
IF 6.4
Menghan Du,Zeqi Sun,Minzhen Xie,Siqi Gu,Yongchao Chen,Qi Wang
Rapid identification of the structure and active components of traditional Chinese medicine is still limited due to low extraction efficiency, long time requirements and imprecision. To overcome these limitations, we developed a novel surface-enhanced Raman spectroscopy (SERS) base, Ag@Cs nanoparticles, which enhanced the effectiveness of Raman spectroscopy and enabled trace analysis of drugs. Cesium ions have remarkable chemical activity and photoelectric effects. Here, the novel approach involved using cesium ions as focusing agents and thiosulfate ions as cleaning agents. Its distinctive feature lies in the introduction of cesium ions as focusing agents, which facilitates the surface modification of silver nanoparticles, leading to the formation of hotspots and consequently achieving highly repetitive and high signal-to-noise ratio Raman spectra. The results demonstrated that Ag@Cs, compared with the original method, greatly enhanced the spectral signal magnitude of glycogens and glycosides in twenty kinds of Potentilla discolor Bge. Additionally, Ag@Cs SERS enabled the analysis of glycogens and glycosides in twenty samples, which showed obvious and different corresponding characteristic peaks to distinguish them. Furthermore, by analyzing the peak spectrum information and principal component analysis of glycogen and glycoside components from ten different origins, the quality of the different origins can be determined. Compared with the original method, it was proved that Ag@Cs base could overcome the influence of citrate ions and was effectively applied in the SERS detection of flavonoids containing fluorescent components. Therefore, this method has potential applications in Chinese medicine quality control and testing technology.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·Vol. 416
·期 10
·2515-2525
·
IF 3.8
Peng Ruiying,Qi Wenchen,Deng Ting,Si Yanmei,Li Jishan
The ultrasensitive detection of hepatitis C virus (HCV) nucleic acid is crucial for the early diagnosis of hepatitis C. In this study, by combining Ag@Au core/shell nanoparticle (Ag@AuNP)-based surface-enhanced Raman scattering (SERS) tag with hybridization chain reaction (HCR), a novel SERS-sensing method was developed for the ultrasensitive detection of HCV nucleic acid. This SERS-sensing system comprised two different SERS tags, which were constructed by modifying Ag@AuNP with a Raman reporter molecule of 4-ethynylbezaldehyde, two different hairpin-structured HCR sequences (H1 or H2), and a detection plate prepared by immobilizing a capture DNA sequence onto the Ag@AuNP layer surface of the detection wells. When the target nucleic acid was present, the two SERS tags were captured on the surface of the Ag@AuNP-coated detection well to generate many “hot spots” through HCR, forming a strong SERS signal and realizing the ultrasensitive detection of the target HCV nucleic acid. The limit of detection of the SERS-sensing method for HCV nucleic acid was 0.47 fM, and the linear range was from 1 to 10 5 fM. Graphical
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-9
·
IF 5.479
Lai Huasheng,Shang Wenjuan,Yun Yuyin,Chen Danjiao,Wu Liqian,Xu Fugang
Magnetite (Fe 3 O 4 ) spheres acting as a core were evenly decorated with gold nanoparticles (AuNPs) and coated with a shell of a metal organic framework (MOF) of type MIL-100(Fe). The resulting hybrid nanomaterial of type Fe 3 O 4 -Au@MIL-100(Fe) hybrid is shown to be a viable new SERS substrate. The integration of magnetic core, build-in plasmonic gold nanoparticles and a MOF shell endows the Fe 3 O 4 -Au@MIL-100(Fe) with highly efficient magnetic separation and enrichment ability, abundant interparticle hotspots, and significant chemical enhancement effect. This leads to a large enhancement, and greatly improved reproducibility of the SERS signals as shown for Malachite Green (MG) and the fungicide thiram. MG in solution can be quantified with a 50-fold lower detection limit (0.14 nM for peak at 1398 cm −1 ) and largely improved reproducibility (RSD = 9%, 1398 cm −1 ) when compared to the use of (a) AuNPs anchored on MIL-100(Fe) (RSD = 27%, 1186 cm −1 ), or (b) AuNPs embedded in MIL-100(Fe) (RSD = 36%, 1398 cm −1 ). The method was applied to the quantitation of MG and thiram in spiked water samples. The lower limits of detection are 4.4 nM for MG (1398 cm −1 ) and 15 nM for thiram (1380 cm −1 ), respectively, and signals’ RSDs are 13% (1398 cm −1 ) and 5% (1380 cm −1 ) for MG and thiram, respectively. The substrate is recyclable.
Schematic illustration of the preparation and SERS molecule sensing application of Fe 3 O 4 -Au@MIL-100(Fe) hybrid. PMMA: poly(methacrylic acid; BPEI: branched poly(ethyleneimine); BTC: 1,3,5-tricarboxybenzene.
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