JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2026
·
IF 3.1
Meichen Liu,Yalin Yu,Yifei Jing,Yingxia Guo,Chuya Wang,Hongyu Xue,Hong Liu,Lei Yin,Meiyun Shi
While polyethylene glycol (PEG) is an extensively utilized pharmaceutical polymer with established biocompatibility and regulatory acceptance, the in vivo pharmacokinetics of its bifunctional derivatives, such as α,ω-dipropionic acid polyethylene glycol (PA-PEG-PA), remain largely unexplored. This study presents the development and validation of a highly sensitive and selective UPLC-MS/MS method for the accurate quantification of PA-PEG 12 -PA in various biological matrices, utilizing a straightforward protein precipitation protocol. After intravenous administration in rats (10 mg/kg), PA-PEG 12 -PA demonstrated rapid clearance (t 1/2 = 3.99 ± 1.06 h). Tissue distribution analysis revealed a pronounced affinity for renal accumulation, with kidney concentrations (16473 ± 881 ng/g at 2 h) substantially surpassing those in the liver and lungs, followed by rapid depletion within 24 h. Excretion studies indicated renal clearance as the dominant pathway, with 55.93 % of the administered dose recovered unchanged in urine over 72 h, while fecal excretion was minimal (1.76 %). This work provides the first comprehensive pharmacokinetic and biodistribution profile of PA-PEG 12 -PA, underscoring its renal-driven clearance and tissue disposition. The findings offer crucial insights for the rational design of PEGylated drug delivery systems, and the robust UPLC-MS/MS assay established herein serves as a valuable tool for characterizing polymeric excipients in biological environments.
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.2
Xiaoxiao Li,Ying Chen,Zhengyu Jin,Yuxiang Bai
The GH70 family 4,3/6-α-glucanotransferase efficiently converted readily digestible (α1→4) glycosidic bonds into (α1→3) or (α1→6) glycosidic bonds, thus serving as an effective enzymatic tool for preparing starch-based dietary fiber. Amino acid residues, including V1409, Q1397, T1366, K1362, H1369, and N1418 of Limosilactobacillus fermentum NCC 2970 GtfB 4,3-α-glucanotransferase (Lf2970 GtfB), were found via sequence conservation analysis and tunnel engineering for further analysis. Mutants from K1362, T1366, H1369, and N1418 at the tunnel entrance showed increased enzyme activity and catalytic efficiency but reduced molecular weight of products. The mutant Q1397P within the tunnel increased the proportion of (α1→6) bonds in the products to 7.9% and (α1→3) bonds to 26.3%, from the wild-type of 1.2% and 21.9%, respectively. These results indicated that amino acid residues located at the tunnel entrance regulated the enzyme activity and product size of Lf2970GtfB, while the Q1397 site within the tunnel regulated the type and proportion of glycosidic bonds.
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FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Yuanyuan Li,Ning Guan,Haifu Jia,Yougui Cheng,Xiaoyan Pei,Biao Liu,Jingzhou Xiao,Yujun Jiang,Qianyu Zhao,Ling Guo
To expand the application potential of α-lactalbumin (α-LA) in functional foods, this study investigated the interaction mechanisms and physicochemical property of noncovalent complexes formed between α-LA and soybean lecithin (SL). SL addition quenched the intrinsic fluorescence of α-LA, increased hydrophobic group exposure, and induced a transition of protein secondary structure from ordered to more disordered conformations. Negative ΔG, ΔH, and ΔS values indicated that the binding process was spontaneous and exothermic. Molecular docking and dynamics simulation revealed the binding site resides within the hydrophobic region of α-LA, primarily driven by hydrogen bonds (Trp60) and hydrophobic interactions (Leu105, Lys108). Density functional theory reveals that the complexes involved weak interactions characterized by electron cloud transfer between α-LA and SL, resulting in a HOMO-LUMO energy gap of 0.125 eV. At an SL concentration of 0.4 mg/mL, the α-LA-SL complexes exhibited the smallest particle size (453.80 ± 4.82 nm), highest emulsifying activity index (30.09 ± 0.09 m 2 /g), and highest emulsion stability index (77.88 ± 2.85 min). Meanwhile, protein oxidative modification and surface tension were reduced. Correlation analysis indicated that the decrease in surface tension was a key factor in enhancing the complex's functionality, while interfacial adsorption-related protein oxidative changes were closely related to its functional enhancement. Oil-in-water model emulsions stabilized by α-LA-SL complexes were further prepared, and the 0.4 mg/mL SL group showed improved storage stability during accelerated storage. Overall, α-LA and SL formed noncovalent complexes with improved structural and functional properties, providing a theoretical basis for constructing food emulsion systems.
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BIOLOGICAL TRACE ELEMENT RESEARCH
·
2026
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IF 4.3
Yang Wenyan,Ma Lu,Hu Ting,Zhang Lei,Wu Kewei,Chen Hualin,Zhang Dandan,Luo Peng
Geochemical fluoride pollution is a global public health issue affecting a wide range and large population. The major health hazard from long-term fluoride exposure is skeletal fluorosis, a highly disabling and irreversible bone damage disease with no effective early interventions available to date. Given reactive oxygen species (ROS) accumulation is a key event in fluoride-induced bone damage, we established a sodium fluoride-exposed rat model. During the phase when fluoride induced ROS accumulation and mild bone damage in rat bone tissue, we administered Rosa roxburghii Tratt juice (RRTJ)—a natural food-medicinal homologous plant with antioxidant properties—to explore its intervention effect and potential mechanism against fluoride-induced bone damage. Results showed RRTJ inhibited fluoride-induced decreases in bone mineral density and trabecular number in male rats. Since enhanced bone resorption mediated by osteoclast overactivation is the initiating link of these fluoride-induced pathological changes, this study further found RRTJ suppressed fluoride-induced increases in osteoclast activity in male rat bone tissue, specifically reducing osteoclast numbers in bone tissue and lowering serum levels of bone resorption markers CTX and TRAP-5b. This effect is achieved by RRTJ scavenging fluoride-induced ROS accumulation in bone tissue, restoring ROS/TNF-α/NF-κB pathway homeostasis, and thereby inhibiting the overexpression of osteoclast activation-related genes tartrate-resistant acid phosphatase and cathepsin K. In conclusion, this study confirms RRT inhibits fluoride exposure-induced bone damage in male rats by regulating ROS/TNF-α/NF-κB pathway homeostasis. These findings provide new perspectives on the mechanism of fluoride-induced bone damage and, more importantly, reveal a potential novel intervention strategy.
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高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 21
·215705
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IF 3.953
Zhen Mao,Hui Li,Na Gan,Zili Suo,Huan Zhang,Qiang Zhao
Fixed carbon source and different dopants are mainly used to study the effect of heteroatoms on the structure and properties of carbon dots (CDs). As reactants, some dopants with conjugated structure and high nitrogen content may have important contributions to the structure and properties of doped CDs in addition to providing heteroatoms. Herein, to study the effect of fixed dopant on the structure and properties of CDs, three different CDs were synthesized using nicotinamide (NAA) and three common α-hydroxy acids (4–5 carbon atoms), and the optimal conditions were determined by orthogonal experimentation. Transmission electron microscopic micrographs showed that the average size of CDs based on nicotinamide are relatively large, up to 19.40 nm. X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy demonstrated that these CDs have graphite nitrogen and several functional group structures. Ultraviolet–visible absorption spectra, fluorescence emission spectra, and fluorescence lifetime illustrated that these CDs have similar emission centers (460–470 nm) and fluorescence processes. The influence of carbon source on the surface structure of CDs was determined by systematically analyzing the response of these CDs in different pH ranges. DFT calculations revealed the distribution characteristics of the electrons in the excited state at the HOMO and LUMO energy levels of CDs. All the above characterizations and calculations proved that NAA is a desirable dopant with an important contribution to the structure and properties of CDs.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
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IF 7.312
Xiangjuan Ma,Yan Yan,Qizhou Dai,Jianxian Gao,Shengjue Liu,Yijing Xia
This study focused on the electrochemical oxidation of Acid Blue 9 (AB 9) under direct and pulse current modes using a Ti/SnO 2 -Sb/α,β-Polytetrafluoroethylene-Fe-PbO 2 (PTFE-Fe-PbO 2 ) anode. The results showed that 99.0% of AB 9 and 68.9% of COD could be eliminated at initial pH of 5, initial AB 9 concentration of 100 mg L -1 , pulse frequency of 4000 Hz, current density of 20 mA cm −2 and pulse duty cycle of 50% after 120 min of electrolysis. The pulse electrochemical oxidation of AB 9 at the investigated conditions followed pseudo-first-order kinetics. Compared with the direct current mode, 76.0% of energy consumption could be saved at pulse current mode. The effect of mass transfer and electrode distance on the pulse electrochemical oxidation of AB 9 was also investigated in terms of mass transfer coefficient and effectiveness factor, the results showed that the reduced electrode distance was beneficial to improve the mass transfer and oxidation efficiency, as well as the energy efficiency. LC-MS analysis revealed that benzenesulfonate group, C-N and C-S bonds in AB 9 molecule were initially cleaved under the attack of ⋅OH, then some carboxylic acids were formed and eventually mineralized to CO 2 and H 2 O. Therefore, pulse electrochemical oxidation with a PTFE-Fe-PbO 2 electrode might be a promising alternative to enhance the practicability of electrochemical treatment for dye wastewater.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2021
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IF 5.316
Shuai Wang,Changhao Yao,Yifeng Cai,Yang Yang,Huiyuan Ma,Baojiang Jiang,Jun Ma
In this study, α-Fe 2 O 3 /rGO was smoothly synthesized by a one-step method and employed to activate persulfate (PS) for the degradation of Bisphenol A (BPA) under sunlight. During the degradation experiment, the degradation rates of BPA for PS, α-Fe 2 O 3 /8% rGO, and α-Fe 2 O 3 /8% rGO/PS were 6.6%, 47.6%, and 99.1%, respectively, indicating that the introduction of α-Fe 2 O 3 /rGO and PS significantly enhanced the photocatalytic efficiency because that photocatalytic process and PS activation coupling system not only inhibited the recombination of photo-induced carrier pairs, but also reduced the energy consumption of PS activation. Moreover, electron spin resonance spectroscopy (ESR) and trapping experiments were utilized to explore the active species generated during the reaction. The results showed that •OH and •SO 4 2- were the principal oxidative species in this reaction system. These results showed that Fe-loaded rGO has great potential for activating PS to decompose pollutants in water.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Zeming Wang,Zihao Wang,Wei Li,Yeqing Lan,Cheng Chen
The hybrids of Co 3 O 4 anchored on different crystallographic MnO 2 (α-MnO 2 @Co 3 O 4 , β-MnO 2 @Co 3 O 4 , γ-MnO 2 @Co 3 O 4 , and δ-MnO 2 @Co 3 O 4 ) were synthesized and applied in peroxymonosulfate (PMS) activation for the degradation of sulfisoxazole (SIZ). Various characterizations were conducted to explore the physicochemical properties of the hybrids, and their catalytic activities were compared. The SIZ degradation followed pseudo-first-order kinetics, and the rate constants ( k obs ) were 0.1135, 0.0307, 0.1080, and 0.0285 min −1 for α, γ, β, and δ-MnO 2 , respectively. The introduction of Co 3 O 4 greatly improved the catalytic activities of the MnO 2 hybrids, and more than 94.7% of SIZ was degraded within 20 min. The k obs was in the order of γ-MnO 2 @Co 3 O 4 (0.3952 min −1 ) > α-MnO 2 @Co 3 O 4 (0.1573 min −1 ) > β-MnO 2 @Co 3 O 4 (0.1536 min −1 ) > δ-MnO 2 @Co 3 O 4 (0.1319 min −1 ). Obviously, the synergy of Co 3 O 4 and MnO 2 weakened the differences of catalytic performance of MnO 2 caused by their microstructures among α, β, and δ-MnO 2 . Due to its large surface area, γ-MnO 2 @Co 3 O 4 exhibited the most excellent catalytic performance for the SIZ removal. All the hybrids also displayed superior stability and reusability, and the degradation rates of SIZ maintained were above 97% during five consecutive cycles. The reactive oxygen species (ROS) including SO 4 •− , • OH, and 1 O 2 were responsible for the SIZ degradation in the system of MnO 2 @Co 3 O 4 /PMS, which was confirmed by electron paramagnetic resonance (EPR) technique and scavenger tests. Twelve intermediates of the SIZ degradation were identified via HPLC-TOF-MS 2 . Combined with the results of characterizations together with ROS identification, the possible catalytic mechanisms and pathways of the SIZ degradation were proposed. This study may shed some light toward the application of different crystallographic MnO 2 hybridized with other transition metals in PMS activation .
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MATERIALS RESEARCH BULLETIN
·
2021
·
IF 4.641
Yanzhen Yin,Zhen Wang,Maochen Zhu,Yue Zhang,Jianhong Li,Chao Dou,Shijia Sun,Bing Teng,Yang Che,Degao Zhong
A single-phased warm white phosphor of α-Sr 2 V 1.98 P 0.02 O 7 : 0.5 % Eu 3+ with low correlated color temperature (CCT) and high color rendering index (CRI) was synthesized by high temperature solid phase method. By changing the ratio of PO 4 3− /VO 4 3− , the emission intensity of VO 4 3− and the energy transfer efficiency of VO 4 3− →Eu 3+ can be effectively tuned, and the full-spectrum warm white light emission of phosphors in the range of 400−700 nm can be realized. Moreover, the temperature-dependent emission spectra and thermal quenching properties were investigated in detail. On this basis, the WLED prototype devices were packaged with the synthesized phosphors α-Sr 2 V 1.98 P 0.02 O 7 : 0.5 % Eu 3+ and 365 nm near-ultraviolet (NUV) chips and high-quality warm white light emission with CCT = 3188 K and CRI Ra = 93.5 were achieved. These findings provide new insights into exploring single-phased white phosphors for NUV-pumped warm w-LEDs with high CRI property and low CCT value.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Shuang Qiu,Xueping Wang,Mingyang Zan,Zhanzhong Wang,Leping Dang
The melting crystallization is a crucial way to isolate unsaturated fatty acids from peony seed oil , mainly composed of oleic acid (OA), linoleic acid (LA), and α-linolenic acid (ALA). The solid-liquid phase behaviors of oleic/linoleic acid (OA-LA), oleic/α-linolenic acid (OA-ALA), and linoleic/α-linolenic acid (LA-ALA) binary systems at the cooling and melting stage were investigated by employing differential scanning calorimetry (DSC). The solid-liquid melting phase diagrams of the three binary systems were constructed by the thermal analysis and ideal liquidus lines simulated with thermodynamic models, which interpreted the eutectic behaviors, polymorphic transition, and intermolecular interaction in different binary systems. The eutectic behavior appeared in the OA-LA and OA-ALA binary systems at the OA concentration less than 40% and 20%, respectively, and in LA-ALA binary systems at any proportion. Based on the enthalpy of the three binary systems, the enthalpy plots and the melting Gibbs free energy were provided and predicted, respectively. This work provided insights into the separation of peony seed oil or other plants oil-based fatty acid mixtures, hence suggesting great potential for the optimization of crystallization technology.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2020
·
IF 4.65
Weijun Liang,Senlin Wang,Yanxiu Zhang,Jian Gu,Kaida Zhu,Jing Zhong,Xinru Wang
β-Ni(OH) 2 with good crystallinity is grown on the surface of the flower-like α-Ni(OH) 2 to form a 3D α@β-Ni(OH) 2 material by a two-step hydrothermal method . The synthesized β-Ni(OH) 2 is nanometer size and strong alkali stability, while the α-Ni(OH) 2 has a large specific surface, high specific capacity and good electrical conductivity , so there are multiple synergistic effects between the β-Ni(OH) 2 coating and the α-Ni(OH) 2 core. Coupled with a 3D flower-like structure, the obtained α@β-Ni(OH) 2 electrode shows a high capacity of 431 mAh g −1 at a current density of 1 A g −1 . Furthermore, we use the α@β-Ni(OH) 2 electrode and the activated carbon (AC) electrode to assemble an asymmetric supercapacitor (ASC) device. This ASC shows the excellent energy density of 53 Wh kg −1 at a power density of 875 W kg −1 and capacity retention rate of 78% after 5000 cycles. Finally, metal hydride (MH) is used to replace the AC negative electrode, and a cell with a high energy density of 100.17 Wh kg −1 at a power density of 424.75 W kg −1 is obtained. The results show that α@β-Ni(OH) 2 electrode has better electrochemical performance than commercial electrodes and has application prospects in energy storage.
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FUEL
·
2020
·
IF 5.578
Limo He,Song Hu,Xiaofei Yin,Jun Xu,Hengda Han,Hanjian Li,Qiangqiang Ren,Sheng Su,Yi Wang,Jun Xiang
Fe-modified Ni/Al 2 O 3 catalysts with Fe/Ni molar ratios of 0, 0.5, 1 and 2 were prepared to promote the co-production of H 2 and carbon nanotubes (CNTs) during steam reforming of toluene as a tar model compound. After the addition of Fe, toluene conversion increased from 55.1% of Ni/Al 2 O 3 to 73.6% of F1N1A (Fe/Ni ratio of 1), and H 2 yield over F1N1A reached the maximum of more than 2000 mmol/(g-cata). Multi-walled carbon nanotubes with average diameter of 10–30 nm were generated and follow tip-growth mechanism. Compared with Ni/Al 2 O 3 , the addition of Fe remarkably increased the amount and quality of CNTs which had longer length and less tortuosity. These dual promoting effects on H 2 and CNTs after the addition of Fe were attributed to the strong interaction between Ni and Fe in Fe-Ni alloy. Fe-Ni alloy had the high activity for the decomposition performance of toluene and H 2 O to generate more H 2 and intermediates for improving the reforming reaction. Meanwhile, the high catalytic decomposition activity supplied more carbon species and the formation of Fe-Ni alloy enhanced the generation of metal carbide for promoting CNTs growth. Besides, the deposition of amorphous carbon was suppressed and the interaction between Ni particle and catalyst support was weakened after the addition of Fe, which kept the activity of catalyst and promoted the tip-growth of CNTs.
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危险品化学品经营许可证（带存储）