JACS Au
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2025
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IF 8.7
Zhuo Zhang,Mingchen Lv,Jiaxi Xu,Yaping Liu,Jinlong Qin,Zhen Fan,Jianzhong Du
Alzheimer’s disease (AD) is an irreversible neurodegenerative disease characterized by abnormal performance in memory, cognition, and language, and it imposes a heavy economic burden worldwide. Amyloidosis and oxidative stress are highly associated with AD progression, yet limited clinical drugs are available at present. Nanozymes exhibit diverse enzyme-mimetic activities and have attracted widespread attention as a promising alternative candidate for scavenging reactive oxygen species to maintain the oxidation–antioxidation balance in cells. Neurotoxic amyloid-β (Aβ) aggregation is also a critical event in AD pathology. The development of dual-targeting nanomaterials with antiamyloidosis ability and enzyme-mimicking activity is expected to be a promising strategy for the treatment of amyloidosis and reactive oxygen species-mediated AD progression. Here, bimetallic-peptide framework nanozymes (CuZn-PEP NZs) with amyloid-β (Aβ) attenuating ability, multiple enzyme-mimicking properties, and broad-spectrum reactive oxygen species scavenging capacity were endowed to inhibit Aβ fibrillization, disaggregate Aβ fibrils, and scavenge Aβ fibril-induced reactive oxygen species. An obvious inhibitory effect on Aβ fibrillization and a disaggregation effect on Aβ fibrils were observed after treatment with CuZn-PEP NZs. Meanwhile, the cytotoxicity of Aβ fibrils toward PC12 cells was significantly reduced by CuZn-PEP NZs. Meanwhile, CuZn-PEP NZs with multiple redox pairs exhibit superoxide dismutase, catalase, and glutathione peroxidase-mimicking enzyme properties simultaneously, which further display cytoprotective effects against Aβ fibril-induced reactive oxygen species and mitochondrial damage. Besides, cellular studies verified that CuZn-PEP NZs possess excellent biocompatibility and blood–brain barrier penetration capacity. Overall, these bimetallic-peptide framework nanozymes represent a promising perspective for attenuation of amyloid-β aggregation and reactive oxygen species simultaneously, which highlights the potential of nanozymes for the treatment of amyloidosis and reactive oxygen species-mediated AD progression.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Jianglan Yuan,Congxin Lei,Yuchen Duan,Hanxin Zeng,Junzhe Ma,Dongliang Chen,Xu Kang
Heme is widely recognized as an effective iron supplement. In vitro experiments suggested a relatively low bioaccessibility of free heme due to its strong hydrophobicity. The study prepared a β-lactoglobulin-heme nanocomplex (β-Lg-He) to improve the bioaccessibility of heme. The results indicated that heme could spontaneously bind to β-Lg mainly through hydrogen bonds and van der Waals forces to form water-soluble and uniform nanocomplexes. Simulated gastrointestinal digestion experiments showed that bioaccessibility of heme in β-Lg-He was more than 80%, while that of free heme was only about 8.0%, and the serum iron levels of mice in β-Lg-He groups were higher than those in heme groups and control groups. The results of in vivo experiments revealed that β-Lg-He could have a more beneficial effect on the intestinal microbiota of mice than free heme, which was an additional function for the high-efficiency iron supplement.
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ANALYTICA CHIMICA ACTA
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2025
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IF 6
Wenwu Li,Tingting Xie,Shizhen Gan,Yuanxun Gong,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao
Background Electrochemiluminescence (ECL) biosensors are powerful tools for biomarker detection due to high sensitivity, low background, and programmable signal generation. However, conventional ECL platforms face limitations including reliance on static recognition elements (e.g., antibodies) and susceptibility to denaturation. To address this, we developed a novel β-turn-structured peptide-based ECL biosensor integrating MXene (Ti3C2) nanomaterials with a Ru(dcbpy)32+/AuNPs hybrid. This system targets SERPINE1, a senescence-associated secretory protein and promising early biomarker for lung cancer, leveraging peptide conformational switching for enzyme-free signal amplification. Results The biosensor employs a rationally engineered peptide probe containing: (1) a SERPINE1-specific recognition motif, (2) a central β-turn domain locking the peptide in a catalytically inactive folded state, and (3) a His/Cys-rich tail mimicking peroxidase-like activity. Target binding unfolds the β-turn structure, exposing catalytic residues that accelerate tripropylamine (TPA) oxidation and enhance ECL emission. MXene provides a high-conductivity platform while facilitating efficient peptide immobilization via embedded AuNPs. Under optimized conditions, the sensor achieves ultrasensitive detection of SERPINE1 with a wide linear range (0.05–800 ng/mL), a low detection limit (11.2 pg/mL), and high specificity. It operates without enzymatic amplification and demonstrates excellent reproducibility (RSD = 4.2%) and stability (>85% signal retention after 8 days). Validation in human serum yielded recoveries of 96.3–104.6%. Significance This work introduces the first MXene-assisted ECL biosensor utilizing β-turn peptide conformational switching. The enzyme-free, modular strategy offers clinically adaptable detection of senescence biomarkers for early lung cancer diagnosis. By integrating molecular recognition, structural dynamics, and nanomaterial-enhanced signal transduction, this platform advances programmable biosensing and can be extended to other disease-related targets through peptide redesign, showing broad potential in precision diagnostics.
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Regenerative Biomaterials
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2025
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IF 8.1
Shan Yanbo,Bai Yang,Zhao Lisheng,Zhou Qing,Yang Shuo,Wang Gang,Lei Ye,Lu Yuzheng,Wu Yanbin,Wei Yu,Peng Jiang,He Rujie,Wen Ning,Gu Bin
Reconstructing bone defects remains a significant challenge in clinical practice, driving the urgent need for advanced artificial grafts that simultaneously promote vascularization and osteogenesis. Addressing the critical trade-off between achieving high porosity/strength and effective bioactivity at safe ion doses, we incorporated strontium (Sr) into β-tricalcium phosphate (β-TCP) scaffolds with a triply periodic minimal surface (TPMS) structure using digital light processing (DLP)-based three-dimensional (3D) printing. Systematically screening Sr concentrations (0-10 mol%), we identified 10 mol% as optimal, leveraging the synergy between the biomimetic TPMS architecture, providing exceptional mechanical strength (up to 1.44 MPa at 80% porosity) and facilitating cell recruitment, and precision Sr-dosing to enhance bioactivity. In vitro assays revealed that the Sr-TCP scaffold dose-dependently stimulated osteogenic differentiation and mineralization in mouse osteoblastic cell line (MC3T3-E1) cells, while also significantly enhancing the angiogenic capacity in human umbilical vein endothelial cells (HUVECs). In vivo studies indicated that the scaffold demonstrated synergistic osteogenic and angiogenic effects in rat femoral condylar defects, leading to marked improvements in bone healing. Collectively, this study establishes a novel design paradigm combining biomimetic topology with optimized ionic doping, resolving key limitations of conventional grafts and advancing the development of safe, highly effective biomaterials for vascularized bone regeneration.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Haihui Xing,Xuan Sun,Lei Xing,Qiaoyun Li,Mingjie Puyang,Lihua Liu,Dan Chen
Alzheimer's disease (AD), a prototypical neurodegenerative disorder, is pathologically characterized by amyloid-β (Aβ) deposition, hyperphosphorylated Tau protein accumulation, and oxidative stress cascades. Current therapeutic strategies are constrained by limited blood-brain barrier (BBB) penetration efficiency and insufficient targeting specificity. This study developed a brain-targeting intelligent nanodelivery system (CC-Ang@ECR) based on the natural pleiotropic neuroprotectant ecdysterone (ECR) to synergistically modulate AD pathological networks. The system employs carboxymethyl chitosan (CMCS) as the backbone, functionalized with hydrophobic 4-carboxyphenylboronic acid (CPBA) to construct a ROS-responsive amphiphilic polymer (CMCS-CPBA, abbreviated as CC), and further modified with Ang-2 peptide for targeted engagement of LRP receptors on BBB endothelial cells, enabling efficient brain delivery. The nanocarrier encapsulates hydrophobic ECR via hydrophobic interactions, achieves BBB transcytosis through Ang-2 peptide mediation, and releases ECR precisely in AD lesions via CPBA hydrolysis triggered by elevated ROS microenvironments. In vitro and in vivo studies demonstrate that CC-Ang@ECR effectively scavenges excessive ROS, suppresses oxidative stress, attenuates aberrant Aβ deposition, downregulates pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α), inhibits neuronal apoptosis, and ultimately ameliorates cognitive and motor impairments. This system overcomes the BBB limitations and off-target risks inherent to conventional therapies, providing an innovative solution for synergistic AD therapy.
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FOOD RESEARCH INTERNATIONAL
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2025
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IF 8
Peng Man,Jinghua Pei,Sijia Chen,Xiue Han,Huajiang Zhang
This study explored the effects of ultrasound on β-lactoglobulin (β-LG)/puerarin (PUE) complexes, focusing on structural, digestive, and allergenic properties. Molecular simulations revealed hydrogen bonding as the primary interaction between PUE and β-LG. Ultrasound reduced β-LG crystallinity (via XRD), further enhanced by PUE encapsulation. Moderate ultrasound (300 W) increased thermal denaturation temperature by 8.2 °C (DSC) and enhanced digestibility to 78.3 ± 1.2 % (vs. 62.5 ± 2.1 % in untreated group), while excessive power (500 W) caused structural damage. Ultrasonic treatment increased the β-sheet content of β-LG, led to a reorganization of its tertiary structure. Allergenicity assays demonstrated significant reductions in IgE and IgG binding capacity for 300 W-treated complexes. These findings highlighted moderate ultrasound (300 W) as an effective strategy to modulate functionality and reduce allergenicity in protein-polyphenol colloids, offered insights for food science applications.
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Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Feiyan He,Jilai Gong,Juan Li,Jun Zhao,Huaiyang Zhou,Yewei Wang,Yanzhao Hu
Nanofiltration (NF) membranes are part of the promising technologies for treating antibiotic wastewater. However, current NF membranes are constrained by the trade-off between the water permeability and selectivity. To address this challenge, a (2-Hydroxypropyl)-β-cyclodextrin (OH-β-CD) incorporated into nitrogen-doped quantum dots (N-GQDs) aqueous phase to form the membrane (N-CD 0.1 ). The integration of the OH-β-CD manipulated the structural properties of selective layer and improved the water permeability. This was attributed to the fact that OH-β-CD not only provided an additional priority water channel, but also regulated the interfacial polymerization process of the membrane by slowing down the diffusion rate of N-GQDs. In addition, the hydrophilicity and electronegativity of the membrane were optimized by OH-β-CD. The results showed that the N-CD 0.1 membrane achieved a high antibiotic rejection (Ciprofloxacin: 98.28 %, Norfloxacin: 97.9 % and Ofloxacin: 97.46 %). The N-CD 0.1 membrane had a remarkable water permeability of 15.82 L⋅m −2 ⋅h −1 ⋅bar −1 , which was 2.05-fold that of the N-GQDs membrane. Furthermore, the N-CD 0.1 membrane exhibited good high-pressure stability. When the operating pressure was 3 ∼ 7 bar, the N-CD 0.1 membrane still had a high Ciprofloxacin rejection (> 97 %). Therefore, by the addition of OH-β-CD, the water permeability of NF membrane could be further improved almost without sacrificing the rejection of Ciprofloxacin, which provided a green and efficient strategy for the fabrication of high-performance NF membranes.
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JOURNAL OF FLUORESCENCE
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2024
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IF 2.6
Huang Xiaoli,Chang Le,Ge Jianxin,Wang Ping,Yin Rui,Liu Guanqi,Wang Guopin
Colorectal cancer was one of the major malignant tumors threatening human health and β-Gal was recognized as a principal biomarker for primary colorectal cancer. Thus, designing specific and efficient quantitative detection methods for measuring β-Gal enzyme activity was of great clinical test significance. Herein, an ultrasensitive detection method based on Turn-on fluorescence probe (CS-βGal) was reported for visualizing the detection of exogenous and endogenous β-galactosidase enzyme activity. The test method possessed a series of excellent performances, such as a significant fluorescence enhancement (about 11.3-fold), high selectivity as well as superior sensitivity. Furthermore, under the optimal experimental conditions, a relatively low limit of detection down to 0.024 U/mL was achieved for fluorescence titration experiment. It was thanks to the better biocompatibility and low cytotoxicity, CS-βGal had been triumphantly employed to visual detect endogenous and exogenous β-Gal concentration variations in living cells with noteworthy anti-interference performance. More biologically significant was the fact that the application of CS-βGal in BALB/c nude mice was also achieved successfully for monitoring endogenous β-Gal enzyme activity.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Yeting Tu,Zhenglong Chen,Jialun Lin,Peiru Jiang,Qingling Nie,Ruiyun You,Yudong Lu
Hydroxyethyl cellulose (HEC), a biomolecule with good water solubility, pH stability and biocompatibility, is widely used in safety - critical fields like food and pharmaceuticals. Stimulants abuse is a major challenge in sports competitions, where urine is usually used as an analyte, so there is an urgent need to establish a non-invasive, hygienic and immediate detection method. Surface-enhanced Raman spectroscopy (SERS) is a highly sensitive, non-destructive and rapid detection method, which is expected to be used for the rapid detection of stimulants, therefore, this study synthesized a hydrogel SERS substrate based on hydroxyethyl cellulose. The HEC - Ag NPs/PAAM hydrogel (HAP hydrogel) was prepared simply and efficiently, with a rich network structure that could sequester small molecules. Meanwhile, the hydrogel has hydrophilic groups that can adsorb β-adrenergic stimulants in sweat. The incorporation of cellulose and silver nanoparticles (Ag NPs) provides the sensor patch with excellent antimicrobial properties and good biocompatibility. SERS technology is capable of quantitatively analyzing agonists in sweat (detection limit of clenbuterol is 9.4 × 10 −9 g/L). HAP hydrogels sensor exhibit excellent sensitivity and stability during the detection process. This property is crucial for expanding SERS substrates' use in detecting sweat agonists.
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APPLIED SURFACE SCIENCE
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2025
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IF 6.9
Chen Zhang,Chuan Ding,Mingyue Li,Jinyang Zhou,Tao Yuan,Zhemin Shen
The infrared energy of the sun’s rays accounts for more than 40 %. Heterostructures were constructed to enable broad-spectrum response of photocatalysts, and enhance the utilization of sunlight, especially that of infrared lights. A robust heterostructured upconversion composite material decorated with Ag nanoparticles (AgNPs), β-NaLuF 4 @ NaLuF 4 :Yb 3+ ,Tm 3+ @ AgNPs@BiOBr (NLF@NLFT@Ag@Bi), was built through a multi-step hydrothermal method. NLF@NLFT@Ag@Bi demonstrates enhanced photocatalytic activity under UV–Vis-NIR, Vis-NIR, and NIR irradiation compared to pure BiOBr and NLF@NLFT@Ag/BiOBr mixture, achieving 99 % Rhodamine B (RhB) degradation in 0.5 h (UV–Vis-NIR) and 96 % in 3 h (NIR), with degradation over 80 % for Bisphenol A (BPA), Norfloxacin (Nor), and Methyl Orange (MO) in 3 h (UV–Vis-NIR). The upconversion luminescence (UCL) of NLF@NLFT@Ag is tuned by its core–shell structure and AgNPs’ plasmonic effect, while NLF@NLFT@Ag@Bi’s enhanced photocatalysis arises from boosted UCL and AgNPs-facilitated Förster resonance energy transfer (FRET) between NLFT and BiOBr. The material’s efficacy in infrared-driven photocatalysis offers a prospective approach for extending catalyst response ranges. It greatly enhances degradation effects of organic pollutants under sun’s rays.
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FOOD CHEMISTRY
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2025
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IF 9.8
Qingchun Zhang,Hao Zhu,Rui Wang,Jianfei He,Christos Ritzoulis,Owen Griffith Jones,Weilin Liu,Wei Tang,Jianhua Liu
Effect of prior hydrolysis and subsequent 36 h glycation on the ability of amyloid-like fibrils derived from β-Lactoglobulin (FG 36 Hβ-Lg) to provide physicochemical stability to fish oil emulsions was systematically determined under varying pH, ionic strength, thermal, and storage conditions. In vitro digestion studies coupled with a Caco-2 cell model investigation evaluated the emulsions' cellular uptake, transmembrane transport, and antioxidant properties. GC–MS analysis of the post-digestion micellar phase revealed that emulsification enhanced the apparent permeability of eicosapentaenoic acid (EPA). FG 36 Hβ-Lg conferred superior EPA transport efficiency and the strongest cellular antioxidant effects on emulsions, compared to proteins modified by hydrolysis or glycation alone, or lacking fibrillar structure. Parallel reaction monitoring showed that FG 36 Hβ-Lg emulsions upregulated key regulatory proteins involved in lipid metabolism and inflammation, such as HMGCR and NFKB1. These findings demonstrate that modified β-Lactoglobulin fibrils improved physical and oxidative stability of emulsions and show promise to enhance bioavailability of omega-3 fatty acids.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
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IF 12.6
Shijin Xu,Naidi Yang,Fangning Du,Ziying Zhang,Yin Zhang,Yixuan Zhang,Jiawei Liang,Yihan Zhao,Jiajun Zhang,Ziwei Zhang,Xiaoxuan Han,Zena Chen,Zhiqiang Zhou,Shu Zhang,Lin Li,Ying Lv
Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies, marked by extensive stromal fibrosis and a profoundly immunosuppressive, immune-excluded tumor microenvironment (TME) that hampers the efficacy of immune checkpoint blockade. Although photodynamic therapy (PDT) can induce immunogenic cell death (ICD) and stimulate anti-tumor immunity, its effectiveness against PDAC is limited by insufficient immune activation and persistent stromal-mediated immunosuppression. To address these challenges, we develop a liposomal nanodrug that co-encapsulates a mitochondrial-targeted photosensitizer (MP) and a TGF-β receptor inhibitor (LY2109761) to synergize PDT with PD-1 checkpoint blockade. MP selectively accumulates in mitochondria and, upon light activation, amplifies mitochondrial reactive oxygen species production, inducing mitochondrial damage. This damage triggers the release of mitochondrial DNA and damage-associated molecular patterns, activating the STING pathway and eliciting potent ICD and anti-tumor immune responses. Simultaneously, liposome-mediated delivery of LY2109761 mitigates stromal desmoplasia and reverses TGF-β-driven immune suppression, enhancing effector T cell infiltration and activity. In murine PDAC models, this dual-action strategy transforms the immune-cold TME into an immune-inflamed phenotype, sensitizing tumors to PD-1 therapy and leading to pronounced tumor regression and prolonged survival. Our findings present a promising nanodrug-based approach to remodel the fibrotic and immunosuppressive TME of PDAC and enhance immunotherapeutic outcomes.
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