LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Chao Du,Zhenzhen Hui,Na Li,Kaiyue Liu,Yong Wang,Ping Wang,Shuyang Sun
This investigation sought to identify antioxidative peptides derived from Meretrix meretrix L. (MML) throughout simulated gastrointestinal digestion (SGD) via in vitro and in silico strategies. The study demonstrated that hydrolysates from MML captured at various SGD phases showcased robust antioxidative activity. These hydrolysates also displayed a protective role against hydrogen peroxide-induced stress in Raw 264.7 cells. Notably, hydrolysates sourced from the intestinal phase of digestion (MMLHs-I) markedly inhibited the reduction of cell viability, limited the generation of reactive oxygen species under oxidative stress conditions, and promoted the activity of key antioxidant enzymes including superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, thus amplifying the cells' antioxidative defense system. Through nLC-MS/MS analysis, 105 peptides tracing back to 18 parent proteins were pinpointed in MMLHs-I, with 18 of these peptides previously reported in the BIOPEP-UWM database. Subsequent in silico analysis, alongside empirical antioxidant activity assays, pinpointed the peptide WLV as an exceptional antioxidative agent, demonstrating an IC 50 of 0.125 mg/mL in the ABTS assay and an impressive 15.18 μmol Trolox/mg in the ORAC assay. The outcomes highlight MML's promise as a novel source of antioxidative peptides, positioning peptide WLV as a potentially valuable addition to functional foods designed to combat oxidative stress.
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PROGRESS IN ORGANIC COATINGS
·
2024
·
IF 6.5
Zhenhua Zhang,Yangyang Cao,Jieru Wan,Yanli Wang,Rongrong Chen,Rumin Li,Dalei Song,Tao Zhang,Jingyuan Liu,Jun Wang
Nanocontainers loaded with corrosion inhibitors as fillers can endow the organic coating with self-healing abilities. However, the number of nanocontainers that can be added to the coating is limited, and the number of corrosion inhibitors that can be accommodated by the nanocontainers is also limited. These considerably mitigate the anti-corrosion properties of the self-healing coating. Herein, a self-healing double-layered composite coating (MK-VK/EP), l -methionine (L-met)-loaded dendritic silica (KCC-1) nanocontainers modified epoxy coating (EP) as the bottom layer (MK/EP) and vanillin (Van)-loaded KCC-1 modified EP as the top layer (VK/EP), was fabricated on the surface of carbon steel. The results of electrochemical impedance spectroscopy (EIS) show that the low frequency impedance modulus (|Z| 0.01Hz ) of MK-VK/EP is nearly two orders of magnitude higher than that of MK/EP or VK/EP in 3.5 wt% NaCl solution after 45 days. Furthermore, the self-healing rate (r self-healing ) of the scratched MK-VK/EP coating (S-MK-VK/EP) is still much higher than that of the scratched MK/EP or VK/EP in the last two stages of immersion. Meanwhile, there exists no apparent corrosion after 12 h of immersion from the optical image of S-MK-VK/EP. The results show that MK-VK/EP can provide better self-healing corrosion protection performance than the single coating (MK/EP or VK/EP). Hence, the synergistic effect of different corrosion inhibitors can greatly improve anti-corrosion abilities of the coating, which will provide an effective approach for corrosion protection of metal in marine environment.
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MACROMOLECULAR BIOSCIENCE
·
2025
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IF 4.1
Xiaoyu Liu,Jiayi Jiang,Hailei Liu,Fei Liu,Huarong Shao,Shaojuan Chen,Shaohua Wu
An integrated strategy that combines innovative electrospinning technique with traditional hot-stretching post-treatment is designed and implemented to generate high-molecular-weight poly(l-lactic-acid) (hmwPLLA, Mw = 2 80 000 Da) electrospun nanofiber-constructed yarns (ENCYs). The internal fiber diameter within the hmwPLLA ENCYs is found to increase gradually with the increase of hmwPLLA solution concentration. The hmwPLLA ENCY generated from a concentration of 10% (w v −1 ) is demonstrated with uniform morphology with an average fiber diameter of 737.7 ± 72.2 nm and an average yarn diameter of 454.9 ± 3.5 µm. Compared with the unstretched hmwPLLA ENCY, increasing the hot-stretching temperature can significantly enhance the fiber orientation and crystallinity. Moreover, the mechanical properties of stretched ENCYs are obviously enhanced compared with the unstretched control. The fiber orientation and crystallinity of stretched ENCYs are also found to be significantly improved with the increase of hot stretching rate, further resulting in the obvious increase of breaking strength and Young's modulus. Importantly, the braided textiles made from stretched hmwPLLA ENCYs exhibited great biocompatibility by effectively guiding the cell alignment and supporting the cell adhesion and proliferation. In summary, the high performance hmwPLLA ENCYs show great potential for the future design and development of advanced biomedical textiles.
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ENVIRONMENTAL POLLUTION
·
2025
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IF 7.3
Xindong Yang,Chenan Dai,Guoxin Zheng,Shaojie Ding,Juan Wu,Qinghua Zhou,Anping Zhang,Jianqiang Sun
Following the restrictions on bisphenol A (BPA), the production and environmental release of bisphenol analogues (BPs) have increased. However, knowledge about the occurrence of bisphenol analogues other than BPA, especially in farmland soils and edible plants, remains limited. This study investigated the occurrence, contamination characteristics, and human health risks of eight bisphenol analogues in paired soil-plant samples from areas near factories in eastern China. Results indicated that the concentrations of Σ 8 BPs in the collected soil and plant samples ranged from 1.4 to 897.1 ng/g dw and 2.5 to 586.2 ng/g dw, respectively. BPA, bisphenol AF (BPAF), bisphenol F (BPF), and bisphenol S (BPS) were the primary components of BPs, with BPA having the highest detection frequency (74 %). In addition, a positive correlation was observed between the root concentration factor and the log K ow of BPs ( R 2 = 0.471, P < 0.05), whereas the translocation factor exhibited a negative correlation with the log K ow ( R 2 = 0.405, P < 0.05). The hazard index (HI) values of BPs in paired soil-plant samples were <1, suggesting that the current contamination levels of BPs in soils and plants are unlikely to pose significant health risks to humans. However, potential risks from long-term exposure require careful monitoring. This study offers new insights into the spatial distributions and contamination status of BPs in farmland soils and plants, highlighting the environmental behavior and health risks of other bisphenol analogues.
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JOURNAL OF FOOD AND DRUG ANALYSIS
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2024
·Vol. 32
·期 4
·506
·
IF 2.6
Tian-Shuang Xia,Sheng-Yan Xu,Li-Yong Lai,Yi-Ping Jiang,Na-Ni Wang,Hai-Liang Xin
Bitter acids (BA) are main component of Humulus lupulus L. (hops). They are known for beer brewing and have various biological and pharmacological properties, especially the bone-protective effect confirmed by our previous in vivo study. Here we aimed to elucidate the anti-senior osteoporosis (SOP) effect of BA on osteoblasts and explore its underlying mechanism. In vitro SOP model was established by D-galactose (D-gal) injured osteoblasts, and the bone formation markers and apoptosis level were measured. mCherry-EGFP-LC3 adenovirus infection and autophagic markers including beclin1 and LC3 proteins were detected to investigate the autophagy level in osteoblasts. To further verify whether BA play the bone-protective role through regulating autophagy, the autophagy inhibitor 3-MA was used, and the cell proliferation, ALP activity, bone mineralization, apoptosis rate and SA-β-gal staining areas were measured. Finally, the protein expressions of AKT/mTOR signaling pathway were detected by Western blotting, and AKT agonist SC79 and mTOR agonist MHY1485 were used to further study the mechanism of BA on AKT/mTOR-mediated autophagy. The results showed that BA stimulated osteoblastic differentiation and inhibited apoptosis proteins Bcl-2/Bax in D-gal-treated osteoblasts. BA also increased the expression of autophagic markers beclin1 and LC3-II/LC3-I in D-gal-treated osteoblasts. mCherry-EGFP-LC3 autophagic double fluorescent adenovirus showed BA promoted the generation of autolysosomes and autophagosomes in D-gal-injured osteoblasts, indicating that BA might prevent osteoblastic bone loss through activating autophagy. Autophagy inhibitor 3-MA was used to further verify whether BA played the bone-protective role via regulating autophagy. The results revealed the promotion effects of BA on proliferation, ALP activity, and mineralized nodule formation in D-gal-injured osteoblasts were eliminated after autophagy blocking with 3-MA, and the inhibitory effects of BA on apoptosis rate and SA-β-gal staining areas were also eliminated. Moreover, BA reduced the phosphorylation levels of AKT, mTOR, p70S6K, and 4EBP in AKT/mTOR pathway, and the promotion of BA on the autophagic markers was blocked after the activation of AKT and mTOR by SC79 and MHY1485. In conclusion, it was the first time to demonstrate that BA improved cell activities and bone formation in aging osteoblasts, and revealed the mechanism of BA against SOP in osteoblasts was activating AKT/mTOR-mediated autophagy.
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Biotechnology Journal
·
2024
·Vol. 19
·期 4
·2300557
·
IF 3.2
Han-Yu Liu,Feng Qian,Hai-Min Zhang,Qian Gui,Yao-Wu Wang,Pu Wang
The halogenase-based catalysis is one of the most environmentally friendly methods for the synthesis of halogenated products, among which flavin-dependent halogenases (FDHs) have attracted great interest as one of the most promising biocatalysts due to the remarkable site-selectivity and wide substrate range. However, the complexity of constructing the NAD + -NADH-FAD-FADH 2 bicoenzyme cycle system has affected the engineering applications of FDHs. In this work, a coenzyme self-sufficient tri-enzyme fusion was constructed and successfully applied to the continuous halogenation of L -tryptophan. Sp FDH was firstly identified derived from Streptomyces pratensis , a highly selective halogenase capable of generating 6-chloro-tryptophan from tryptophan. Then, using gene fusion technology, Sp FDH was fused with glucose dehydrogenase (GDH) and flavin reductase (FR) to form a tri-enzyme fusion, which increased the yield by 1.46-fold and making the coenzymes self-sufficient. For more efficient halogenation of L -tryptophan, a continuous halogenation bioprocess of L -tryptophan was developed by immobilizing the tri-enzyme fusion and attaching it to a continuous catalytic device, which resulted in a reaction yield of 97.6% after 12 h reaction. An FDH from S. pratensis was successfully applied in the halogenation and our study provides a concise strategy for the preparation of halogenated tryptophan mediated by multienzyme cascade catalysis.
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ACS Agricultural Science & Technology
·
2025
·
IF 2.9
Huiqian Wang,Dongru Wang,Qiuping Zheng,Yuan He,Qian Yang,Jiani Du,Jiawei Wang,Xinhua Zhan
Polyvinyl chloride microplastics (PVC-MPs) pollution is drawing increasing attention, especially due to concerns about the environmental safety of the diverse additives that they contain. To date, few ecotoxicity data are available for PVC MP-derived chemicals. Here, wheat (Triticum aestivum L.) seedling roots were used to comparatively evaluate the toxicity of three commonly studied PVC MP-derived chemicals (2,4-dimethyl-6-s-hexadecylphenol─Irganox 1076 and tris (2,4-ditert-butylphenyl) phosphate─Irgafos 168-ox, and erucamide─Eru). These chemicals were evaluated individually and in combination. Exposure to these chemicals caused dose-dependent reductions in root dry weight (0.39%–19.29%) and root length (0.11%–8.15%). While Irganox 1076 and Eru had minimal impact on root activity and the antioxidant system, Irgafos 168-ox and its mixture induced significant elevations of antioxidant enzymes (SOD, CAT, and APX) activities and antioxidants (AsA and GSH) concentrations in the roots, enhancing the antioxidant level. Our findings indicate that the coexposure of PVC MP-derived chemicals exerts an additive effect on antioxidant response inhibition. Irgafos 168-ox showed stronger effects with the maximum toxic concentration of 10 mg L–1, whether alone or in additive mixtures, due to its higher potential for root accumulation and oxidative stress induction. These results highlight the need for further research into ecological risks of PVC MP-derived chemicals, particularly under combined exposure scenarios.
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FUEL
·
2025
·
IF 7.5
Zhe Yin,Ziwen Wang,Jun Wang,Binglin Tang,Guoping Yu,Qiuyu Wang,Huanyu Chen,Kangzheng Li,Zhichao Zhang,Qingcheng Luo,Fuqin Zhang,Bo Lv
The use of photoreforming to treat plastics and degrade plastics to small molecular organic matter is an emerging approach to solve white pollution in the ocean and produce new energy. Structurally defective g-C 3 N 4 (COCN) is compounded by adding ascorbic acid during the synthesis of graphitic carbon nitride (g-C 3 N 4 ). L-cysteine is added to compose L-CdS. By constructing a heterojunction, we synthesize the ternary composite COCN/L-CdS/Ni 2 P. The hydrogen production rate of COCN/L-CdS/Ni 2 P reaches 29.11 mmol/g/h at 5 h, which is 7.52 times and 727.7 times of L-CdS and COCN, respectively. During the production of hydrogen, polylactic acid (PLA) is degraded to pyruvate. This study demonstrates that COCN/L-CdS/Ni 2 P can not only photoreformate plastics in seawater into small molecular organics, but also efficiently produce H 2 using seawater as a hydrogen source. This has a significant role to treat plastic pollution in seawater and product clean energy.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jian-Guo Zhang,Hao-Xiang Wu,Ai Zhang,Kiran Thakur,Fei Hu,Zhao-Jun Wei
This study compared the effects of ultrasound-assisted extraction (UE), Soxhlet extraction (SE), and steam distillation (SD) on volatile organic compounds (VOCs) and flavor profiles of Piper nigrum L. varieties (Vietnamese pepper, VP; Kampot pepper, KP; Dapo pepper, DP). Using electronic nose, GC–MS, and UPLC analyses, UE showed superior retention of VOC diversity and aromatic complexity, while SE maximized piperine retention (e.g., 4551.35 mg/100 g in VP). SD reduced inter-varietal flavor differences, homogenizing volatile characteristics. Despite methodological variations, key aromatic traits of pepper varieties remained distinguishable. KP processed via UE exhibited the highest terpene content (2503.22 mg/100 g), highlighting its aromatic potential. GC–MS data enabled accurate classification of extraction-based flavor profiles via orthogonal partial least squares discriminant analysis. Nine odorants were identified using odor activity value (OAV) and variable importance in projection (VIP). This study provides insight into method-driven flavor differentiation, VOC characterization, and guides strategies for pepper product development.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Jiahui Gong,Mengfei Tian,Mengying Zhang,Xiaolong Yang,Yujiao Song,Hongying Ge,Wenxue Sun,Chunying Li,Chunjian Zhao
In this study, a novel polydopamine modified carboxymethyl chitosan molecularly imprinted resin (CMCS-MIR) was synthetized for selectively adsorbed and separated epicatechin (EC) from Rumex acetosa L. The preparation process involved modifying the surface of DM-130 resin to form the polydopamine (PDA) layer firstly, which facilitated an increase in adsorption sites. Subsequently, carboxymethyl chitosan (CMCS), a green functional monomer, was utilized along with glutaraldehyde as a crosslinking agent and EC as a template molecule to synthesize the CMCS-MIR. The synthesized CMCS-MIR exhibited an adsorption capacity of 53.30 mg·g −1 within 6 h and exhibited excellent selectivity and affinity towards EC (IF = 3.31). Moreover, the CMCS-MIR maintained stable adsorption performance and reusability with the adsorption efficiency remaining above 90 % in the fifth cycle. What's more, the CMCS-MIR can be used as a novel column filler in solid-phase extraction (SPE) to separate EC from extracts of Rumex acetosa L. roots. The adsorption recovery rate of EC is 89.56 %, the desorption recovery rate reaches 88.02 %. The DFT simulation and NMR experiments indicated that the predominant adsorption mechanism was due to the strong hydrogen bonding interactions. This work offers significant insights into the development of green molecularly imprinted materials with excellent selectivity and recognition capabilities.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2025
·
IF 2.9
Chunhua Zhang,Bowen Li,Xiang Gao,Wei Xiao,Mingfang Chen,Qilong Bian
This work demonstrates a wireless electroenzymatic platform using carbon microsphere electrodes co-immobilized with a rhodium catalyst and lactate dehydrogenase (LDH) for integrated NADH regeneration and pyruvate-to-L-lactate conversion in a bipolar electrochemical system. The 3D "aggregate reactor" architecture, functionalized via diazonium grafting, Rh coordination, and covalent enzyme coupling, enables spatial coupling of cofactor recycling and biocatalysis, while HPLC revealed a L-lactate production rate of 0.04 mM cm-2 h⁻¹ under 12.5 V cm⁻¹ with a plenty of carbon beads attributed to enhanced mass transport. This wireless, scalable design eliminates wiring constraints and offers modular adaptability for sustainable synthesis of chiral chemicals.
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MATERIALS RESEARCH BULLETIN
·
2025
·
IF 5.7
Yue Lan,Xiaogang Zhou,Ruting Zheng,Jianyue Li,Min Wang,Dandan Wang,Shiying Li,Yuqiang Xiao,Jiaqi Hu,Meiling Yang,Lingling Li,Mingyue Wang,Jianming Wu,Die Gao
Novel carbon dots (CDs-1) were synthesized using Sanguisorba officinalis L., as a precursor and a deep eutectic solvent (DES) as both solvent and dopant. For comparison, CDs-2 were prepared under identical conditions but using water as solvent. Chemical analyses reveal that two CDs possess well-defined crystalline structures and are rich in diverse functional groups. Notably, DES-mediated synthesis endows CDs-1 with advantageous features compared to CDs-2, including higher N and Cl contents, enhanced hydrophilicity, and superior optical properties. To evaluate their potential biomedical applications, antioxidant capacities of them were investigated through in vitro and in vivo assays. Results demonstrated that both CDs exhibit potent free radical scavenging activity, strong KMnO 4 reduction capability, and notable anti-cellular oxidative damage effects. Importantly, CDs-1 show superior performance in enhancing oxidative stress resistance at nematode level. Furthermore, owing to the exceptional optical characteristics, CDs-1 hold great promise for bioimaging applications and sensitive detection of 2,4,6-trinitrophenol (TNP).
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危险品化学品经营许可证（带存储）