SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Mingcong Gao,Jiawei Li,Lei Qiu,Xinyi Xia,Xia Cheng,Feifei Xu,Guanhong Xu,Fangdi Wei,Jing Yang,Qin Hu,Yao Cen
Perovskite quantum dots (PQDs) are extremely unstable in ambient air due to their inherent structural instability, which limits the wide application of PQDs. In this work, silicon-coated CsPbBr 3 PQDs (CsPbBr 3 @SiO 2 ) was synthesized via a simple method. The SiO 2 coating effectively isolated PQDs from water and oxygen in the environment, which were the main elements that destroyed the structure stability of PQDs. The synthesized CsPbBr 3 @SiO 2 can be stored in water for more than 2 months and posessed wonderful dispersibility in aqueous solution. The fluorescence intensity remained unchanged within 7 days and only decreased by 11.9 % within 2 months. We found that CsPbBr 3 @SiO 2 was extremely sensitive to environmental pH, and the fluorescence intensity decreased with the reduction of pH. In addition, an excellent linear relationship with pH value in the range of 1.0 ∼ 5.0 was achieved. As we all known that glucose can be catalyzed by glucose oxidase to produce gluconic acid and hydrogen peroxide, in which a good deal of protons were produced and the pH was gradually lowered. Since CsPbBr 3 @SiO 2 was stable to water and oxygen, and sensitive to ambient pH, we applied CsPbBr 3 @SiO 2 to the detection of glucose. CsPbBr 3 @SiO 2 showed fantastic selectivity and sensitivity to glucose, and the detection limit can even reach 18.5 μM. Furthermore, CsPbBr 3 @SiO 2 was successfully applied to the detection of glucose in the human serum with satisfactory performance.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Huike Dong,Guanghua Lu,Xiaoping Wang,Peng Zhang,Haohan Yang,Zhenhua Yan,Jianchao Liu,Runren Jiang
Perfluorooctanoic acid (PFOA) is widely distributed in nature, particularly in aquatic environments. Its bioaccumulation and toxicity in aquatic organisms can be affected by both the chemical status of PFOA in water and the physiology of the organism. However, research on the patterns of these effects is scarce. In this study, we investigated the influence of aqueous pH (pH 6, acidic; pH 7.5, neutral; pH 9, basic) and fish sex on PFOA uptake, clearance, and biochemical effects in crucian carp ( C. auratus ) using flow-through exposure. In the 17-d kinetic experiment, PFOA bioaccumulation adhered to a uniform first-order model in which PFOA uptake rates from water to blood and liver in acidic conditions were faster than those in other conditions, indicating possible acidic pH influence on PFOA uptake. PFOA clearance rates in these compartments of males were slower than in females, which was attributed to the notably stronger expression of Oat2 (organic anion transporter 2, responsible for reabsorption) in the kidneys of males. Similar responses were observed for peroxisome proliferation-related biomarkers at different pH levels and in different sexes. These biochemical responses were driven by the internal concentrations of PFOA. The inhibition acetylcholinesterase activity in the fish brain was closely linked to changes in P-glycoprotein content, demonstrating a protective relationship. Collectively, an aqueous pH lower than 7.5 might affect the uptake of PFOA by fish. The clearance discrepancies between the sexes highlight the importance of anion carriers for ionizable organic compounds in aquatic organisms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Zexiang Zheng,Xing Yang,Yifan Zhang,Weiqiang Zu,Minna Wen,Taisheng Liu,Changren Zhou,Lihua Li
To achieve the pH-responsive release of metformin in tumor acidic microenvironment, we prepared OHA-Met by covalently grafting metformin (Met) onto oxidized hyaluronic acid (OHA) through imine bonds, and then prepared carboxymethyl chitosan (CMCS)/OHA-Met drug loaded hydrogels. The CMCS/OHA-Met hydrogels showed the in-situ injection performance. At pH = 7.4, the cumulative release rate of metformin from CMCS/OHA-Met20 hydrogel was 42.7 ± 2.6 % in 6 h, and the release tended to balance after 72 h. At pH = 5.5, the release kept constant and the cumulative release rate was 79.3 ± 4.7 % at 6 h, showing good pH-responsive behavior. Metformin induced apoptosis of MCF-7 cells through the caspase 3/PARP pathway. CMCS/OHA-Met20 hydrogel could effectively kill MCF-7 cells, while reducing the cytotoxicity of free metformin to L929 cells. In vivo breast cancer recurrence experiments showed CMCS/OHA-Met20 hydrogel could achieve local injection and pH-responsive smart drug delivery at the tumor resection site, inhibiting breast cancer recurrence. Compared with direct administration, CMCS/OHA-Met20 hydrogel reduced the metformin dosage, frequency of administration and systemic side effects.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Haibin Guo,Yanqing Xia,Ke Feng,Xiaowei Qu,Cuilian Zhang,Feng Wan
Background Metal–organic frameworks (MOFs) have attracted intensive research interest in the biomedical field because of their unique properties. However, in order to realize the high loading capacity and therapeutic efficacy, it is still urgent to develop a multifunctional MOFs-based nanoplatform.Materials and Methods Herein, a pH/near-infrared (NIR) dual-responsive drug delivery system based on zeolitic imidazolate framework-8 (ZIF-8) is constructed for synergistic chemo-photothermal therapy and dual-modal magnetic resonance (MR)/photoacoustic (PA) imaging. The doxorubicin hydrochloride (DOX) is embedded into ZIF-8 through one-pot method, and the resultant ZIF-8/DOX is then successively modified with polydopamine, Mn ions and poly(ethylene glycol). The obtained ZIF-8/DMPP is systematically characterized, and both its in vitro and in vivo biological effects are evaluated in detail.Results The ZIF-8/DMPP possesses a high drug-loading content of 18.9% and displays appropriate size and morphology. The pH-dependent degradation and drug release behavior of prepared ZIF-8/DMPP are confirmed. Importantly, the results demonstrate that the photothermal effect of ZIF-8/DMPP under NIR laser irradiation can significantly accelerate its drug releasing rate, further improving the intracellular drug concentrations. Thereafter, the augmented chemotherapeutic efficiency by photothermal effect against cancer cells is verified both in vitro and in vivo. Besides, the favorable MR and PA imaging capacity of ZIF-8/DMPP is also evidenced on the tumor model.Conclusion Taken together, the surface engineering of ZIF-8-based nanocarrier in this work offers a promising strategy for the multifunctional MOFs-based drug delivery system.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2023
·
IF 10.2
Ka Hong Wong,Zhaopei Guo,Di Jiang,Xingzhi Zhou,Lizhu Lin,Denggao Zhao,Meiwan Chen
Nano drug delivery systems have made significant progress in delivering anticancer drugs camptothecin (CPT). However, many challenges for CPT delivery remain, including low drug loading efficiency, premature drug leakage, and poor cellular internalization. Herein, we report a novel dual-sensitive polypeptide-based micelle with remarkably high drug loading of CPT for cancer therapy. This self-assembled micelle possesses the following essential components for CPT: (1) pH-sensitive PEG (OHC-PEG-CHO) for prolonging blood circulation and allowing biocompatibility by shielding the cationic micelles, which can be detached under the tumor acidic microenvironment and facilitates the cellular uptake; (2) polypeptide polylysine-polyphenylalanine (PKF) synthesized via ring-opening polymerization for micelle formation and CPT analogue loading; (3) dimeric CPT (DCPT) with redox-sensitive linker for increasing CPT loading and ensuring drug release at tumor sites. Interestingly, the linear-like morphology of PEG-PKF/DCPT micelles was able to enhance their cellular internalization when compared with the spherical blank PKF micelles. Also, the anticancer efficacy of DCPT against lung cancer cells was significantly improved by the micelle formation. In conclusion, this work provides a promising strategy facilitating the safety and effective application of CPT in cancer therapy.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AAPS PHARMSCITECH
·
2022
·Vol. 24
·期 1
·1-14
·
IF 4.026
Wang Xiaoling,Du Jiangyue,Zhou Feng,Ye Qing,Chen Ying,Sun Dujuan,Chen Haimin,Lv Yuanyuan,Sun Xiaoyi
Adequate delivery of therapeutic agents to their intended molecular targets is crucial in tumor therapy. Versatile drug carriers need to overcome the challenges coming from the systemic circulation, membrane barriers, and endo-lysosomal degradation. Herein, hyaluronic acid-conjugated polydopamine (HA-PDA)-shelled mesoporous silica nanoparticles encapsulated with doxorubicin (MSNs-DOX) were successfully fabricated for targeted tumor therapy. Compared with reported studies focusing on the pH-sensitive release in tumors, we especially revealed the significant role of lysosomal release in DOX nuclear accumulation. After active targeting and CD44-mediated endocytosis in tumor cells, the PDA layer of the nanoparticles would be peeled off to trigger drug release owing to MSNs gatekeeper in acidic lysosomes. Subsequently, DOX molecules passively diffused into nuclei. The intracellular DOX transportation was evidenced by DOX accumulation in nuclei, lysosomal location of nanoparticles, and lysosome acidification inhibition test. After discharging of the cargoes from nanoparticles, PDA shells from residual nanoparticles were able to produce localized hyperthermia under NIR irradiation entrapped in lysosomes, inducing synergistic chemo-photothermal effect. Under NIR treatment, HA-PDA@MSNs-DOX presented a prominent tumor inhibition rate without obvious side effects. This study indicated the potent nuclear delivery and synergetic chemo-photothermal therapy achieved by HA-PDA-shelled MSNs. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2023
·
IF 14
Yanbin Zhao,Peng He,Junyan Yao,Mei Li,Bin Wang,Linyuan Han,Zhihai Huang,Chao Guo,Jing Bai,Feng Xue,Yu Cong,Weihua Cai,Paul K. Chu,Chenglin Chu
Although biodegradable polymer coatings can impede corrosion of magnesium (Mg)-based orthopedic implants , they are prone to excessive degradation and accidental scratching in practice. Bone implant-related infection and limited osteointegration are other factors that adversely impact clinical application of Mg-based biomedical implants. Herein, a self-healing polymeric coating is constructed on the Mg alloy together with incorporation of a stimuli-responsive drug delivery nanoplatform by a spin-spray layer-by-layer (SSLbL) assembly technique. The nanocontainers are based on simvastatin (SIM)-encapsulated hollow mesoporous silica nanoparticles (S@HMSs) modified with polydopamine (PDA) and polycaprolactone diacrylate (PCL-DA) bilayer . Owing to the dynamic reversible reactions, the hybrid coating shows a fast, stable, and cyclical water-enabled self-healing capacity. The antibacterial assay indicates good bacteria-killing properties under near infrared (NIR) irradiation due to synergistic effects of hyperthermia, reactive oxygens species (ROS), and SIM leaching. In vitro results demonstrate that NIR laser irradiation promotes the cytocompatibility, osteogenesis, and angiogenesis . The coating facilitates alkaline phosphatase activity and expedites extracellular matrix mineralization as well as expression of osteogenesis-related genes. This study reveals a useful strategy to develop multifunctional coatings on bioabsorbable Mg alloys for orthopedic implants .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Miaomiao Liu,Xiaosen Pan,Yuanjing Gan,Meng Gao,Xinran Li,Zhen Liu,Xiaojun Ma,Mengru Geng,Xiangqi Meng,Ning Ma,Jie Li
Titanium carbide MXene quantum dots (MQDs) possess intrinsic regulatory properties and selective toxicity to cancer cells. Here, MDQs were selected for the modification of hydroxyapatite (HA) microspheres, and MXene quantum dots-modified hydroxyapatite (MQDs-HA) hollow microspheres with controllable shapes and sizes were prepared as bone drug carriers. The results show that the prepared MQDs-HA hollow microspheres had a large BET surface area (231.2 m2/g), good fluorescence, and low toxicity. In addition, MQDs-HA showed a mild storage–release behavior and good responsiveness of pH and near-infrared (NIR). Thus, the MQDs-HA hollow microspheres have broad application prospects in the field of drug delivery and photothermal therapy.
✓ 已验证
DOI
PubMed
高影响因子
Polymers
·
2023
·Vol. 15
·期 13
·2796
·
IF 5
Tao Ming,Tingting Lan,Mingxing Yu,Hong Wang,Juan Deng,Deling Kong,Shuang Yang,Zhongyang Shen
Continuous in vivo monitoring (CIVM) of pH value is essential for personalized medicine, as many diseases are closely related to acid–base imbalances. However, conventional pH meters are limited in their ability to perform CIVM due to excessive blood consumption, large device volume, frequent calibration, and inadequate real-time monitoring. There is thus an urgent need for a portable method for CIVM of pH value. To address this need, we propose a minimally invasive, continuous monitoring solution in the form of an implantable pH microneedle sensor (MNS) in this study. The MNS is based on the integration of an acupuncture needle (AN) and a Ag/AgCl reference electrode. We fabricate the sensor by electrochemically depositing platinum black and gold nanoparticles onto the AN and further modifying it with polyaniline to increase its sensitivity to hydrogen ions. The pH value is obtained by calculating the open circuit voltage between the modified AN and the reference electrode. The resulting MNS demonstrates excellent selectivity and a high nernstian response to pH (−57.4 mV per pH) over a broad range (pH = 4.0 to pH = 9.0). Both in vitro and in vivo experiments have verified the performance of the sensor, showcasing its potential for biomedical research and clinical practice. The MNS provides an alternative to conventional pH meters, offering a less invasive and more convenient way to perform CIVM of pH value. Moreover, this electrochemical implantable sensor based on AN and silver wires provides a simple and sensitive method for continuous in vivo detection of other biomarkers.Keywords:polyaniline;electrochemical sensor;nanocomposites;continuous in vivo monitoring;pH value
✓ 已验证
产品关联
DOI
PubMed
高影响因子
InfoMat
·
2023
·Vol. 5
·期 10
·e12464
·
IF 22.7
Zheng Yu,Luolin Li,Fangzhi Mou,Shimin Yu,Di Zhang,Manyi Yang,Qing Zhao,Huiru Ma,Wei Luo,Tianlong Li,Jianguo Guan
Swarming magnetic micro/nanorobots hold great promise for biomedical applications, but at present suffer from inferior capabilities to perceive and respond to chemical signals in local microenvironments. Here we demonstrate swarming magnetic photonic-crystal microrobots (PC-bots) capable of spontaneously performing on-the-fly visual pH detection and self-regulated drug delivery by perceiving local pH changes. The magnetic PC-bots consist of pH-responsive hydrogel microspheres with encapsulated one-dimensional periodic assemblies of Fe 3 O 4 nanoparticles. By programming external rotating magnetic fields, they can self-organize into large swarms with much-enhanced collective velocity to actively find targets while shining bright “blinking” structural colors. When approaching the target with abnormal pH conditions (e.g., an ulcerated superficial tumor lesion), the PC-bots can visualize local pH changes on the fly via pH-responsive structural colors, and realize self-regulated release of the loaded drugs by recognizing local pH. This work facilitates the development of intelligent micro/nanorobots for active “motile-targeting” tumor diagnosis and treatment.
✓ 已验证
产品关联
DOI
高影响因子
Flexible and Printed Electronics
·
2023
·Vol. 8
·期 2
·025021
·
IF 3.1
Lipeng Huang,Xiaolian Chen,Xinzhou Wu,Zishou Hu,Shuhong Nie,Chenchao Huang,Shuo Zhang,Wenya Xu,Fangfang Pei,Wenming Su,Zheng Cui
Flexible electrochromic technology has gained numerous attentions in flexible smart wearable devices and flexible displays. For large-area flexible electrochromic devices (FECDs), highly conductive transparent electrodes with advanced stability at a prolonged redox cycling process are indispensable. In this work, a silver (Ag)/nickel (Ni) mesh/PH 1000 hybrid transparent film were successfully fabricated by selectively electrodepositing an 800 nm-thick dense metallic Ni layer and coating PH 1000 on an embedded Ag mesh to improve its electrochemical stability. The prepared hybrid transparent film presented high conductivity with a sheet resistance of below 1.5 Ω sq−1 at over 80% optical transmittance. The Ag/Ni mesh/PH 1000 was successfully utilized as current collectors for all-solid-state FECDs, showing fast coloration switching with a bleaching/coloring time of 0.7 s/0.9 s. In addition, the device demonstrated an exceptional electrochemical cycling stability, which could sustain 89% of its initial optical modulation after 25 000 cycles. More importantly, a remarkable mechanical durability was also achieved with a small optical modulation decay of 15% and an invariable response time after 1000 rolling cycles. In addition, uniform coloration were realized on a 6 × 6 cm2 FECD, demonstrating its great potential for applications of next-generation up-scaling FECDs.
✓ 已验证
产品关联
DOI
高影响因子
ACS Omega
·
2023
·
IF 4.1
Hong Shen,Changwei Zhang,Chengzhang Wang,Jianxin Jiang,Fengxia Tang,Chuan Li,Hua Yuan,Xiaoran Yang,Zhenkai Tong,Yi Huang
Liposomes are considered the best nanocarrier for delivering cancer drugs such as chlorin e6 (Ce6) and paclitaxel (PTX). However, the poor stability and non-selectivity release of liposomes may severely limit their further applications. In this study, based on the characteristics of lutein (L) photo-response and orthoester (OE) acid-response, stable and dual-responsive liposomes (Dr-lips) have been prepared. The Dr-lips exhibited a spherical shape with a uniform size of approximately 58.27 nm. Moreover, they displayed a zeta potential ranging from −45.45 to −28.25 mV and showed excellent storage stability, indicating stable colloidal properties. Additionally, they achieved high drug encapsulation rates, with 92.27% for PTX and 90.34% for Ce6, respectively. Meanwhile, under near-infrared (NIR) light at 660 nm, Ce6 plays a key role in accelerating the photodegradation rate of lutein and PEG-OE-L while also enhancing tissue penetration ability. Additionally, Dr-lips loaded with Ce6 and PTX not only displayed excellent pH and photo dual-responsiveness for targeted delivering and releasing but also showed remarkable reactive oxygen species (ROS) generation capacity and impressive anti-tumor activity in vitro. Therefore, it provides a novel strategy for optimizing stability and enhancing their targeted drug delivery of liposome.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）