ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Wenwu Li,Tingting Xie,Shizhen Gan,Yuanxun Gong,Jiayi Zhang,Qianli Tang,Kai Zhang,Xianjiu Liao
Background Electrochemiluminescence (ECL) biosensors are powerful tools for biomarker detection due to high sensitivity, low background, and programmable signal generation. However, conventional ECL platforms face limitations including reliance on static recognition elements (e.g., antibodies) and susceptibility to denaturation. To address this, we developed a novel β-turn-structured peptide-based ECL biosensor integrating MXene (Ti3C2) nanomaterials with a Ru(dcbpy)32+/AuNPs hybrid. This system targets SERPINE1, a senescence-associated secretory protein and promising early biomarker for lung cancer, leveraging peptide conformational switching for enzyme-free signal amplification. Results The biosensor employs a rationally engineered peptide probe containing: (1) a SERPINE1-specific recognition motif, (2) a central β-turn domain locking the peptide in a catalytically inactive folded state, and (3) a His/Cys-rich tail mimicking peroxidase-like activity. Target binding unfolds the β-turn structure, exposing catalytic residues that accelerate tripropylamine (TPA) oxidation and enhance ECL emission. MXene provides a high-conductivity platform while facilitating efficient peptide immobilization via embedded AuNPs. Under optimized conditions, the sensor achieves ultrasensitive detection of SERPINE1 with a wide linear range (0.05–800 ng/mL), a low detection limit (11.2 pg/mL), and high specificity. It operates without enzymatic amplification and demonstrates excellent reproducibility (RSD = 4.2%) and stability (>85% signal retention after 8 days). Validation in human serum yielded recoveries of 96.3–104.6%. Significance This work introduces the first MXene-assisted ECL biosensor utilizing β-turn peptide conformational switching. The enzyme-free, modular strategy offers clinically adaptable detection of senescence biomarkers for early lung cancer diagnosis. By integrating molecular recognition, structural dynamics, and nanomaterial-enhanced signal transduction, this platform advances programmable biosensing and can be extended to other disease-related targets through peptide redesign, showing broad potential in precision diagnostics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Guangzheng Wang,Yisheng Du,Xu Cheng,Weiwei Li,Dandan Li,Chong Xie,Runqiang Yang,Pei Wang
Selectively hydrolyzed soy protein can enhance wheat-based product quality by modulating gluten thermal polymerization. This study examined the effects of β-conglycinin (7S) and glycinin hydrolysate (GH) on gluten rheological and thermal properties, particle size, Raman spectra, and microstructure during heating. Both 7S and GH improved gluten viscoelasticity, with their combined addition (7S/GH) showing the strongest effect. 7S/GH increased the denaturation temperature by 4.94 °C and enthalpy by 43.84 %, while the D50 at 95 °C was 97.99 % higher than the control. These enhancements were linked to reinforced glutenin–gliadin crosslinking and a strengthened gluten network. 7S and GH synergistically promoted disulfide bond formation, buried tryptophan and tyrosine residues, and induced a more compact gluten conformation. Confocal laser scanning microscopy and micro-computed tomography confirmed a denser gluten network after heating. Overall, 7S and GH act synergistically to improve gluten stability, offering theoretical insights for soy protein hydrolysates in wheat-based product quality improvement.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Yingxin Wu,Kainan Hong,Juzhen Yi,Juncheng Shen,Liqun Yang
Surface-enhanced Raman spectroscopy (SERS) shows unique advantages for the study of protein structure in water environment, because it can provide plenty of structural information of proteins in addition to very weak Raman scattering signal of water. To achieve the Raman-enhanced effect of proteins, the challenge is to prompt protein molecules to approach the surface of noble metal (such as Ag and Au). Based on the enzyme–substrate specificity, the Ag nanoparticles-encapsulated amylopectin (Amyp/AgNPs) colloid was prepared as an aqueous SERS substrate to obtain the Raman-enhanced effect of amylase. Dynamic light scattering analysis showed that the hydrodynamic diameter of the Amyp/AgNPs colloid was in the range of 40–700 nm. UV–vis spectroscopy and energy dispersive spectroscopy confirmed the formation of Ag nanoparticles in the Amyp/AgNPs colloid. The Ag nanoparticles in the Amyp/AgNPs colloid were observed as globular homogeneous particles in the sizes ranging from 10 to 20 nm using transmission electron microscopy (TEM). The crystalline Ag nanoparticles were further observed in the high-resolution TEM image. The obviously enhanced Raman bands assigned to the amino acid residues and the secondary structures of α -amylase and β -amylase appeared in their SERS spectra, which were further analyzed following the principal component analysis (PCA) process. The Raman-enhanced mechanism is considered as that the specificity of two amylases with amylopectin in the Amyp/AgNPs colloid may prompt them to approach the surface of Ag nanoparticles and then produce the significant SERS effect. The SERS strategy based on enzyme–substrate specificity is thus anticipated to apply for structural analysis of enzyme.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biology Direct
·
2024
·Vol. 19
·期 1
·1-14
·
IF 5.7
Sun Tong,Yang Zhiguang
Six-transmembrane epithelial antigen of prostate 3 (STEAP3) is associated with the progression of several human malignancies. However, its role in lung squamous cell carcinoma (LUSC) remains unclear. We measured STEAP3 expression in LUSC cell lines and tissues. LUSC cells with stable STEAP3 overexpression and knockdown were obtained through G418 selection. Multiple assays were used to evaluate the malignant phenotypes of LUSC cells and the activation of the β-catenin signaling. The potential transcriptional regulatory factors of STEAP3 were predicted using the JASPAR database, and the correlation between transcription factor AP-2 gamma (TFAP2C) and STEAP3 was analyzed through the GEPIA database. The study evaluated the regulatory relationship between a potential transcription factor and STEAP3 through ChIP and luciferase reporter assays. Additionally, rescue assays were utilized to ascertain whether TFAP2C serves as the upstream regulatory factor of STEAP3, contributing to LUSC progression. Finally, tumor growth and metastasis were evaluated in vivo. STEAP3 expression was notably higher in LUSC, and its overexpression was linked to a poor prognosis. Moreover, STEAP3 overexpression activated the β-catenin pathway, thereby accelerating cell proliferation and metastasis. Conversely, STEAP3 knockdown had an anti-tumor effect in LUSC. Additionally, TFAP2C bound directly to the STEAP3 promoter and positively regulate its expression in LUSC. The anti-tumor effects of TFAP2C knockdown were partially reversed by STEAP3 overexpression. The study indicates that the TFAP2C/STEAP3 axis may be a therapeutic target for LUSC treatment. This enhances our understanding of lung carcinogenesis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2024
·
IF 5.3
Tongguo Huo,Dan Zhu,Jianxin Zheng,Yuan Lin,Yu Dai,Jian Wu
This study systematically investigated for the first time the effects of varying the temperature, pressure, C/Si molar ratio, and H 2 /Si molar ratio on β-SiC coatings produced via chemical vapor deposition (CVD) using the SiCl 4 –CH 4 –H 2 –N 2 system. Thermodynamic theoretical calculations were performed using the HSC Chemistry software. The temperature and C/Si molar ratio considerably affected the morphology, phase, and composition of β-SiC coatings. High-quality β-SiC coatings along the (111) crystal plane exhibiting a uniform surface, high density, low roughness, and good crystallinity were successfully synthesized at 1523.15 K and 200 Pa with a C/Si molar ratio of 1.0 and H 2 /Si molar ratio of 6.5. Additionally, when N 2 was used as the dilution gas instead of Ar, the resulting β-SiC coatings demonstrated reduced roughness, fewer free carbon, increased hardness (37.05 GPa vs. 39.19 GPa), enhanced modulus of elasticity (237.26 GPa vs. 264.24 GPa), and superior coating adhesion (16.48 MPa vs. 18.29 MPa).
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Hong Li,Xiaoqian Lan,Feng Bao,Shuanger Li,Haoran Zhu,Zijuan Zhu,Yadong Li,Mingliang Wang,Caizhen Zhu,Jian Xu
Polyimide (PI), as an ideal interlayer dielectric material, is widely used in traditional communication fields. However, with the rapid development of 5G communication, conventional polyimide exhibits disadvantages such as signal delay and missing and is in urgent need of modification. Based on the β-relaxation theory, we designed and prepared three types of triphenylenediamine-containing monomers with different side group sizes (A-AmM, A-AmP, and A-AmBP) and polymerized them with benzene tetracarboxylic dianhydride (PMDA) to obtain three types of polyimides (MPI, PPI, BPPI), to investigate the effect of the relaxation behavior on the dielectric constant (D k ) of the polyimide. It was also polymerized with 4,4′-(hexafluoroisopropyl)diphthalic anhydride (6FDA) to three fluorinated Polyimides (MFPI, PFPI, BFPPI) to investigate the effect of polar groups on D k and the dielectric loss (D f ). In addition, we also calculate the free volume of polyimide molecules under different side groups by materials studio simulation to further verify the effect of side groups on the D k . The results show that, firstly, with the increase of side group volume, polyimide still maintains excellent thermal stability, and its dielectric constant gradually decreases and mechanical strength gradually increases. Secondly, the dielectric constant of FPIs is significantly lower than that of the PIs due to the low polar group, and the thermal and mechanical properties are almost unchanged. In addition, benefiting from the combined effects of β-relaxation and trifluoromethyl groups, BPFPI exhibits a low D k (2.64@10 GHz) and D f (0.0092@10 GHz), excellent thermal properties (glass transition temperature (T g ) = 282.3 °C, 5 % decomposition temperature (T d5% ) = 537 °C) and excellent mechanical properties (tensile modulus = 1.71 GPa, tensile strength = 134.7 MPa).
✓ 已验证
产品关联
DOI
高影响因子
Antibiotics-Basel
·
2025
·Vol. 14
·期 9
·915
·
IF 4.6
Ru Wang,Kun Mi,Aihua Lu,Chengyang Zhang,Lei Sun,Yuxiang Chen,Yuanhu Pan,Yanfei Tao,Lingli Huang
Background/Objectives: Extended-spectrum β-lactamase (ESBL)-producingEscherichia coliposes a significant global health challenge, as it leads to antimicrobial treatment failure and is associated with elevated mortality rates. The use of β-lactam/β-lactamase inhibitor combinations offers an alternative approach for combating ESBL-producing bacteria. Ceftriaxone (CRO) and sulbactam have been coadministered in the clinical settings; however, discrepancies in their pharmacokinetics raise concerns regarding the rationality of this combination.Methods: This study was designed to investigate the postβ-lactamase inhibitor effect (PLIE) under both static and dynamic conditions, with the aim of supporting the clinical application of this combination.Results: The minimum inhibitory concentration (MIC) of CRO/SBT (2:1 ratio) againstE. coliNCTC 13353 was determined to be 32/16 μg/mL. The PLIEs were determined to be −1.26, −0.57, and 0.37 h at CRO/SBT concentrations ranging from 1/2 MIC to 2 MIC, respectively. The results of CRO concentration, β-lactamase activity,blaCTX-M-15expression, and cell morphology collectively support that SBT exerts PLIEs and protects against the antibacterial activity of CRO. In the dynamic hollow-fiber infection model, CRO monotherapy showed no inhibitory effect onE. coli, whereas CRO/SBT combination therapy rapidly eliminated SBT, achieved comparable bactericidal effects, prolonged CRO exposure, and maintained low β-lactamase activity levels.Conclusions: In conclusion, CRO/SBT exerts an inhibitory effect on enzyme-producing strains by being able to produce PLIE to maintain the inhibition of β-lactamase.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Shuai Wu,Ya Wang,Yunfan Li,Minyao Qiu,Yuan Zhong,Maolan Zhang,Haifeng Yang,Jianshu Li,Wei Wu
Clinical treatment of temozolomide (TMZ)-resistant glioblastoma (GBM) remains a significant challenge. This study aims to develop a multifunctional biomimetic Nano-AUTAC (BM@pPPD/TMZ) that can selectively degrade β-catenin via autophagy, thereby overcoming TMZ resistance in GBM. BM@pPPD/TMZ is capable of penetrating the blood-brain barrier (BBB), targeting GBM lesions via microglia-derived membrane camouflage, and inducing degradation of β-catenin through the autophagy-lysosomal pathway. For improving the selective and efficient degradation of β-catenin, BM@pPPD/TMZ is functionalized with a tri-functional peptide comprising a β-catenin-binding domain, a cathepsin B (CTSB)-cleavable GLFG linker, and a phosphatidylserine (PS)-targeting motif. In the GBM microenvironment, the elevated CTSB triggers linker cleavage, promoting membrane shedding and enhancing the cellular uptake. Meanwhile, the incorporation of the cationic lipid DOTAP promotes autophagosome formation, further facilitating degradation of β-catenin. Both in vitro and in vivo studies demonstrate that BM@pPPD/TMZ is able to effectively penetrate the BBB and accumulate within tumor lesion, concurrently promoting β-catenin degradation and MGMT downregulation. This finding provides a promising nanotherapeutic strategy for overcoming TMZ resistance and improving GBM therapeutic efficacy.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 6
·1809
·
IF 4.412
Jifen Zhang,Yanhua Wang,Jirui Wang,Tao Yi
A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs. This study aimed to develop a new solid NSSPE of puerarin preserving the original microstructure of NSSPE by spray-drying. A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying. The drug release and stability of the optimized solid NSSPE in vitro were also investigated. The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution. The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature. Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE. The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly. The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material. The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.Keywords:nanocrystals;Pickering emulsions;solid dosage form;hydroxypropyl-β-cyclodextrin;spray-drying;puerarin;poorly soluble drugs
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2017
·
IF 3.034
Yongjing Liu,Hongmin Yu,Hua Zhang,Lishuang Yu,Wei Xu
In the present work, four β -cyclodextrin derivatives including neutral (methyl- and hydroxypropyl-) and anionic (carboxymethyl- and sulfated-) β -cyclodextrin derivatives have been tested for the enantioseparation of higenamine by capillary electrophoretic method. To our knowledge, there is no literature about using capillary electrophoresis for the chiral separation of higenamine. To achieve the best resolution in the presence of each β -cyclodextrin derivative, the primary factors affecting separation efficiency, such as buffer pH and concentration of β -cyclodextrin derivatives, were investigated. All enantiomeric separation was performed by 30 mmol/L phosphate buffer with an applied voltage of 20 kV or − 20 kV. Under the optimum conditions, four β -cyclodextrin derivatives (methyl- β -cyclodextrin, hydroxypropyl- β -cyclodextrin, carboxymethyl- β -cyclodextrin and sulfated- β -cyclodextrin) exhibited good separation performances for higenamine with the R s value of 2.3, 5.8, 9.4 and 11.7 respectively. As well as the chiral separation abilities of different β -cyclodextrin derivatives were compared. Moreover, the method for determining R -(+)-higenamine in Nelumbins plumula was proposed employing carboxymethyl- β -cyclodextrin as the chiral selector.
✓ 已验证
DOI
高影响因子
DALTON TRANSACTIONS
·
2013
·Vol. 42
·期 27
·9852-9861
·
IF 3.806
Yuan Wu,Dongmei Yang,Xiaojiao Kang,Ping'an Ma,Shanshan Huang,Yang Zhang,Chunxia Li,Jun Lin
Multifunctional nanocomposites integrating mesoporous and luminescence properties into a single entity are synthesized via a facile and effective approach. Oleic acid capped β-NaYF4:Ce3+/Tb3+ nanoparticles (NPs) are transferred into aqueous solution by cetyltrimethylammonium bromide (CTAB) surfactants, and further encapsulated with uniform mesoporous silica shell followed by the surface modification with poly(ethylene glycol) (PEG), leading to the formation of water-dispersible and biocompatible core–shell structured β-NaYF4:Ce3+/Tb3+@mSiO2-PEG (denoted as NPs@mSiO2-PEG) nanospheres. The as-synthesized nanospheres show a typical mesoporous structure and a green emission under UV irradiation. Doxorubicin hydrochloride (DOX), a widely used anti-cancer drug, is used as a model drug to evaluate the loading and controlled release behaviors of the NPs@mSiO2-PEG in phosphoric acidic buffer solutions (PBS) at different pH values (pH = 7.4 and 5.0). The composite carriers provide a pH-sensitive drug release pattern and the drug releases faster under lower pH value. The endocytosis process of fluorescein isothiocyanate (FITC)-labelled nanospheres is characterized using flow cytometry and confocal laser scanning microscopy (CLSM) against A549 cells. The in vitro cytotoxic effect against A549 cells of the DOX-loaded carriers is investigated in detail. In addition, the extent of drug release can be monitored by the variation of photoluminescence (PL) intensity of β-NaYF4:Ce3+/Tb3+. Considering the good biocompatibility and pH-dependent drug release pattern, such core–shell structured luminescent NPs@mSiO2-PEG nanospheres have potential applications in controlled drug delivery.
✓ 已验证
DOI
PubMed
高影响因子
Theranostics
·
2019
·
IF 8.063
Xu-Qi Kang,Gao-Feng Shu,Sai-Ping Jiang,Xiao-Lin Xu,Jing Qi,Fei-Yang Jin,Di Liu,Yong-Hong Xiao,Xiao-Yang Lu,Yong-Zhong Du
The prevalence of antibiotic resistance and lack of alternative drugs have posed an increasing threat to public health. Here, we prepared β-Ga 2 O 3 :Cr 3+ nanoparticles modified with ICAM1-antibody-conjugated TPGS (I-TPGS/Ga 2 O 3 ) as a novel antibiotic carrier for the treatment of drug-resistant infections. Methods : I-TPGS/Ga 2 O 3 were firstly characterized by measuring particle size, morphology, crystal structure, drug loading capacity, and in vitro drug release behaviors. The in vitro antibacterial activities of I-TPGS/Ga 2 O 3 /TIG were evaluated using standard and drug-resistant bacteria. The internalization of I-TPGS/Ga 2 O 3 was observed by fluorescence confocal imaging, and the expression levels of the efflux pump genes of TRKP were analyzed by real-time RT-PCR. In vitro cellular uptake and in vivo biodistribution study were performed to investigate the targeting specificity of I-TPGS/Ga 2 O 3 using HUEVC and acute pneumonia mice, respectively. The in vivo anti-infective efficacy and biosafety of I-TPGS/Ga 2 O 3 /TIG were finally evaluated using acute pneumonia mice. Results : It was found that TPGS could down-regulate the over-expression of the efflux pump genes, thus decreasing the efflux pump activity of bacteria. I-TPGS/Ga 2 O 3 with small particle size and uniform distribution facilitated their internalization in bacteria, and the TPGS modification resulted in a significant reduction in the efflux of loaded antibiotics. These properties rendered the encapsulated tigecycline to exert a stronger antibacterial activity both in vitro and in vivo . Additionally, targeted delivery of I-TPGS/Ga 2 O 3 mediated by ICAM1 antibodies contributed to a safe and effective therapy. Conclusion : It is of great value to apply I-TPGS/Ga 2 O 3 as a novel and effective antibiotic delivery system for the treatment of drug-resistant infections.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）