JOURNAL OF NATURAL PRODUCTS
·
2023
·
IF 5.1
Jinghan Zhang,Xiao Xu,Yu Zhao,Chunling Ren,Mengzhen Gu,Haili Zhang,Peiye Wu,Yun Wang,Lingyi Kong,Chao Han
The glucose transporter 1 (GLUT1) protein is involved in the basal-level absorption of glucose in tumor cells. Inhibiting GLUT1 decreases tumor cell proliferation and induces tumor cell damage. Natural GLUT1 inhibitors have been studied only to a small extent, and the structures of known natural GLUT1 inhibitors are limited to a few classes of natural products. Therefore, discovering and researching other natural GLUT1 inhibitors with novel scaffolds are essential. Physalis angulata L. var. villosa is a plant known as Mao-Ku-Zhi (MKZ). Withanolides are the main phytochemical components of MKZ. MKZ extracts and the components of MKZ exhibited antitumor activity in recent pharmacological studies. However, the antitumor-active components of MKZ and their molecular mechanisms remain unknown. A cell membrane-biomimetic nanoplatform (CM@Fe3O4/MIL-101) was used for target separation of potential GLUT1 inhibitors from MKZ. A new withanolide, physagulide Y (2), together with six known withanolides (1, 3–7), was identified as a potential GLUT1 inhibitor. Physagulide Y was the most potent GLUT1 inhibitor, and its antitumor activity and possible mechanism of action were explored in MCF-7 human cancer cells. These findings advance the development of technologies for the targeted separation of natural products and identify a new molecular framework for the investigation of natural GLUT1 inhibitors.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2023
·Vol. 107
·期 5
·3219-3229
·
IF 3.9
Hongtao Li,Rongxia Huang,Hua-Tay Lin,Zuchao Du,Yejing Dai
This research investigated the effect of Bi 4 Ti 3 O 12 (BiT) template particles size on the structure and piezoelectric properties of 〈001〉-textured (Bi 0.5 Na 0.5 )TiO 3 (BNT)-based lead-free piezoelectric ceramics. Results of X-ray diffractometer analyses and scanning electron microscopy observations showed that high purity BiT template particles could be obtained with the KCl as the molten salt system. Highly 〈001〉-textured (~87%) BNT-based ceramics prepared with large size BiT templates (7.75 µm) exhibited a high inverse piezoelectric constant d 33 * (1100 pm/N), which was ∼3.38-fold larger than that with 1.91 µm (325 pm/N). In addition, we have found that too small the template size can make it difficult to align the orientation of the template during the casting process, whereas too large the size can create problems such as too much hysteresis (∆ S / S max = 67%). This study has demonstrated that the use of high purity large size BiT template particles could provide a feasible route to fabricate BNT-based textured ceramics with desired electro-strain properties.
✓ 已验证
产品关联
DOI
高影响因子
MICROVASCULAR RESEARCH
·
2021
·
IF 3.514
Yi-hua Zhou,Yu-zhi Tang,Liang-yun Guo,Li-li Zheng,Dan Zhang,Can-ying Yang,Wei Wang
Soluble fms-like tyrosine kinase-1 (sFlt-1), a circulating antiangiogenic protein, is involved in the pathogenesis of atherosclerosis (AS), and the underlying mechanism is still unclear. Here, we attempted to investigate the mechanism of action of sFlt-1 in AS. Human umbilical vein endothelial cells (HUVECs) were treated with oxidized low density lipoprotein (ox-LDL) to induce cell injury. ox-LDL treatment increased LC3-II/LC3-I ratio, Beclin-1 expression and GFP-LC3 puncta in HUVECs, suggesting that ox-LDL may induce autophagic flux impairment in HUVECs. ox-LDL-treated HUVECs displayed a decrease of sFlt-1 levels. Moreover, ox-LDL treatment reduced cell proliferation and elevated apoptosis in HUVECs, which was abrogated by sFlt-1 overexpression. Up-regulation of sFlt-1 repressed the activity of PI3K/AKT/mTOR signaling pathway and enhanced autophagy in HUVECs following ox-LDL treatment. Additionally, sFlt-1 overexpression-mediated increase of autophagy in ox-LDL-treated HUVECs was abolished by 3-methyladenine (autophagy inhibitor). 3-methyladenine abrogated the impact of sFlt-1 overexpression on proliferation and apoptosis in ox-LDL-treated HUVECs. This work confirmed that overexpression of sFlt-1 activated autophagy by repressing PI3K/Akt/mTOR signaling pathway, and thus alleviated ox-LDL-induced injury of HUVECs. Therefore, this study suggests that sFlt-1 may be a potential target for AS treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Redox Biology
·
2026
·
IF 16.2
Zhengyi Chen,Xidan Fu,Chao Liu,Wenkang Jiang,Jiurong Liu,Miao Hao,Ling Fu,Wei Huang,Xiujuan Lang,Xijun Liu,Yumei Liu,Hulun Li,Rui Li,Bo Sun
Aging reshapes hematopoiesis toward myelopoiesis, particularly increased output of classical monocytes, contributing to immunosenescence. However, the underlying mechanisms remain unclear. Here, we identify that aging drives hematopoietic stem cells (HSCs) to progressively commit to a CCR2 + monocyte fate along a defined lineage trajectory, which is executed through a redox-sensitive transcriptional cascade. We find that the age-related decline in bone marrow IGF-1 is the upstream niche signal that releases suppression of the oxidative stress/NF-κB/IRF8 axis, allowing this cascade to redirect early progenitors toward a CCR2 + myeloid-primed fate. Functional assays confirm that cells following this CCR2 + trajectory drive myeloid-biased hematopoiesis and directly contribute to age-associated immunosuppression. Our work delineates a hierarchical pathway from niche decay to lineage redirection, establishing the CCR2 + fate trajectory as a key driver of immunosenescence and a potential therapeutic target.
✓ 已验证
DOI
高影响因子
WATER RESEARCH
·
2023
·
IF 12.8
Di Cao,Xueqi Chen,Jun Nan,Aijie Wang,Zhiling Li
Influences of heavy metal exposure to the organohalide respiration process and the related molecular mechanism remain poorly understood. In this study, a non-obligate organohalide respiring bacterium, Pseudomonas sp. strain CP-1, was isolated and its molecular response to the five types of commonly existed heavy metal ions were thoroughly investigated. All types of heavy metal ions posed inhibitory effects on 2,4,6-trichlorophenol dechlorination activity and cell growth with the varied degree. Exposure to Cu (II) showed the most serious inhibitive effects on dechlorination even at the lowest concentration of 0.05 mg/L, while the inhibition by As (V) was the least with the removal kinetic constant k decreased to 0.05 under 50 mg/L. Further, multi-omics analysis found compared with Cu (II), As (V) exposure led to the insignificant downregulation of a variety of biosynthesis processes, which would be one possible account for the less inhibited activity. More importantly, the inhibited mechanisms on the organohalide respiration catabolism of strain CP-1 were firstly revealed. Cu (II) stress severely downregulated NADH generation during TCA cycle and electron donation of organohalide respiration process, which might decrease the reducing power required for organohalide respiration. While both Cu (II) and As (Ⅴ) inhibited substrate level phosphorylation during TCA cycle, as well as electron transfer and ATP generation during organohalide respiration. Meanwhile, CprA-2 was confirmed as the responsible reductive dehalogenase in charge of 2,4,6-TCP dechlorination, and transcriptional and proteomic studies confirmed the directly inhibited gene transcription and expression of CprA-2. The in-depth reveal of inhibitory effects and mechanism gave theoretical supports for alleviating heavy metal inhibition on organohalide respiration activity in groundwater co-contaminated with organohalides and heavy metals.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2023
·
IF 3.8
Chenhao Wei,Di Gao,Guohao Zhang,Liang Zhao,Jinsen Gao,Chunming Xu
1-Hexene aromatization is a promising technology to convert excess olefin in FCC gasoline to high-value benzene (B), toluene (T), and xylene (X). Besides, the increasing market demand of xylene has put forward higher requirements for new generation of catalyst. For increasing xylene yield in 1-hexene aromatization, the effect of mesopore structure and spatial distribution on product distribution and Zn loading was studied. Catalysts with different mesopore spatial distribution were prepared by post-treatment of parent HZSM-5, including NaOH treatment, Tetra-propylammonium hydroxide (TPAOH) treatment, and recrystallization. It was found the evenly distributed mesopore mainly prolongs the catalyst lifetime by enhancing diffusion properties but reduces the aromatics selectivity, as a result of damage of micropores close to the catalyst surface. While the selectivity of high-value xylene can be highly promoted when the mesopore is mainly distributed interior the catalyst. Besides, the state of loaded Zn was also affected by mesopores spatial distribution. On the optimized catalyst, the xylene selectivity was enhanced by 12.4% compared with conventional Zn-loaded parent HZSM-5 catalyst at conversion over 99%. It was attributed to the synergy effect of mesopores spatial distribution and optimized acid properties. This work reveals the role of mesopores in different spatial positions of 1-hexene aromatization catalysts in the reaction process and the influence on metal distribution, as well as their synergistic effect two on the improvement of xylene selectivity, which can improve our understanding of catalyst pore structure and be helpful for the rational design of high-efficient catalyst.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Yu Miao,Fangzheng Yan,Ye Yuan,Fengrui Yang,Bin Sun,Zhi Wang
Loose nanofiltration membranes (LNMs) prepared by co-deposition of polyphenols and amine-rich polymers has been considered as an effective method for the efficient treatment of textile wastewater. However, the dye/salt selectivity remains unsatisfactory, due to the low free volume with wide distribution caused by the stacking and tight entanglement of polymeric chain segments. A high dye/salt selective LNM was prepared for the first time by rapid co-deposition of high-valent metal-induced microporous polymer framework (HMMP-1) and dopamine (DA). HMMP-1 with high specific area and suitable pore size can provide additional transfer channels for water/salt, breaking the limitation of free volume generated by polymer stacking and further improving perm-selectivity. In addition, the amine-rich HMMP-1 with a flexible polymer framework can be covalently linked to DA by Michael addition/Schiff base reaction, effectively avoiding the selectivity reduction caused by poor interfacial compatibility. The results showed that the pure water permeance of the optimal membrane (LNM-2) was 57.22 LMH·bar −1 , which was 0.65 times higher than that of LNM-1 (without HMMP-1). In addition, LNM-2 exhibited excellent dye/salt selectivity for dye/salt mixtures with different concentrations, reaching >47 (CR/NaCl) and >29 (CR/Na 2 SO 4 ), respectively, which is more than twice of the conventional membranes reported so far. Moreover, the dye/salt selectivity of LNM-2 still maintained at 28 (CR/Na 2 SO 4) and 55 (CR/NaCl) respectively for 12 h, when salt concentration was 10 g L −1 , which fully demonstrated the good stability of LNM-2 and the superiority of HMMP-1 for the design of LNM.
✓ 已验证
产品关联
DOI
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Xiankang Fan,Nan Ling,Chunli Liu,Mingzhen Liu,Jue Xu,Tao Zhang,Xiaoqun Zeng,Zhen Wu,Daodong Pan
In this study, an efficient cholesterol-lowering strain of Lactiplantibacillus plantarum 54–1 was screened and its degradation molecular mechanism was investigated. Furthermore, a novel practical MRS medium for screening cholesterol-lowering lactic acid bacteria (LAB) was developed based on ultrasound treatment. L. plantarum 54–1 was found to have the highest ability to eliminate cholesterol (340.69 ± 5.87 µg/mL). According to SEM and the count of viable LAB results, the morphology of LAB in the cholesterol-containing medium developed in this experiment was close to the normal (full and smooth), and it can grow normally. Metabolomics revealed that L. plantarum 54–1 initially converted a portion of cholesterol to 7α-hydroxy-cholesterol and then to the key metabolite taurine, via the phosphotransferase system. These metabolites were further transformed into L-alanine, L-lysine, N6-Acetyl-L-lysine, (R)-b-aminoisobutyric acid, and 2-oxoarginine, through glycine, serine, and threonine metabolism, citrate cycle, D-arginine and D-ornithine metabolism, lysine degradation, and pyruvate metabolism pathways. Prokaryotic reference transcriptomics found that this may be mainly regulated by the bsh , phnE , ptsP , B0667_RS04545, and B0667_RSRS12300 genes, which was further validated by qPCR. Furthermore, molecular docking results demonstrated that 8 differential metabolites might bind to another portion of cholesterol via PI-PI conjugation and hydrophobic interactions and lower cholesterol via co-sedimentation. This study has strategic implications for developing probiotic powder food that lowers cholesterol.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 11
·1-9
·
IF 5.7
Wang Jing,Shi Lu,Tang Qiaorong,Zhu Xinyu,Wu Mengmeng,Liu Wei,Li Baoxin,Jin Yan
Nucleic acid lateral flow assay (NALFA) with gold nanoparticles (AuNPs) as colorimetric probes have been extensively adopted for point-of-care testing (POCT). However, the sensitivity of NALFA still needs to be improved. Herein, DNA-gold nanoaggregate (DNA-AuNA) was assembled as a signal amplification probe of NALFA for sensitive detection of tumor marker TK1 mRNA. Four functional oligonucleotides with complementary pairs were assembled to form DNA-AuNA that coupled more AuNPs to improve sensitivity. Thus, the limit of detection (LOD) was 0.36 pM, which is lower than that of conventional AuNPs-based NALFA. Moreover, the bioassay showed good reproducibility, stability, and specificity for detecting TK1 mRNA. The detection of TK1 mRNA in human serum was also satisfactory. Therefore, DNA-AuNA-based NALFA provides a sensitive method for portable detection of TK1 mRNA. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2023
·
IF 6
Ruizhe Chen,Yongfeng Liu,Chenyang Yin,Long Wang,Lu Zhang,Jinou Song,Hua Sun
To investigate the influence of 1-nitropropane (1-NP) initiator on the pyrolysis of n-hexane ( n -C 6 H 14 ), molecular dynamics (MD) simulations and synchrotron vacuum ultraviolet photoionization mass spectrometry (SVUV-PIMS) experiments were used to analyze the pyrolysis processes of n -C 6 H 14 and n -C 6 H 14 with 10 % 1-NP added. ReaxFF MD simulations were used to analyze the pyrolysis rates and product distributions of n -C 6 H 14 and 1-NP/ n -C 6 H 14 at different temperatures. The mechanism of action of n -C 6 H 14 pyrolysis initiated by 1-NP was investigated. Additionally, the pyrolysis products of n -C 6 H 14 and 1-NP/ n -C 6 H 14 were identified and quantitatively analyzed using the SVUV-PIMS technique. These experimental results were compared with the ReaxFF MD simulation results to validate the reliability of the simulations and to complement each other. The results indicate that the presence of 1-NP promotes the pyrolysis of n -C 6 H 14 and significantly improves the chemical heat sink of the fuel. The chemical heat sink in the 1-NP/ n -C 6 H 14 system differs from that in the n -C 6 H 14 system by 28.78 kJ/kg at 2103 K. A significant increase in C 2 H 4 production in the pyrolysis products of n -C 6 H 14 with the addition of 1-NP. The pyrolysis of 1-NP/ n -C 6 H 14 yielded approximately twice as much C 2 H 4 as n -C 6 H 14 at 2103 K. The pyrolysis reactions were described by first-order kinetics, and the activation energy for the pyrolysis of 1-NP/ n -C 6 H 14 was determined to be 140 kJ/mol, approximately 48 % lower than that of n -C 6 H 14 . The composition of the pyrolysis products was confirmed by SVUV-PIMS experiments, which were in good agreement with the ReaxFF MD simulations. The mechanism of pyrolysis of n -C 6 H 14 initiated by 1-NP was elucidated: the NO 2 radicals generated by 1-NP react with H radicals of the system to form OH radicals. The primary reaction of n -C 6 H 14 involves the abstraction of H by OH, leading to the formation of C 6 H 13 . Subsequently, C 6 H 13 is further decomposed to form 1-C 3 H 7 , 1-C 4 H 8 , 1-C 5 H 10 and 2-C 6 H 12 .
✓ 已验证
产品关联
DOI
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2023
·
IF 7.5
Ke Li,Mengxia Yan,Haili Wang,Lifen Cai,Ping Wang,Huimin Chen
As a typical high-efficient alkane aromatization catalyst, Pt/KL is prone to deactivation due to the sintering growth of Pt on the outer surface of KL and the channel blockage caused by carbon deposition , which is further exacerbated by the one-dimensional channel structure of KL. Therefore, a series of encapsulated catalysts combining the three-dimensional channel structure advantages of S-1 with the role of potassium are prepared and evaluated. It is shown that the encapsulated catalyst KPt@S-1-02 modified by moderate potassium exhibits extremely superior stability and no significant deactivation is observable after n-heptane aromatization for 180 h under harsh conditions. On the one hand, encapsulation structure enables a strong anti-agglomeration ability of Pt and the topological structure of S-1 endows the encapsulation catalysts less susceptible to channel blockage, which has been demonstrated by HAADF-STEM and N 2 -adsorption. On the other hand, characterizations involving NH 3 -TPD, CO-DRIFT and HAADF-STEM verify that the modification by an appropriate amount of K can inhibit acid sites of catalysts and improve Pt dispersion and electron density, thus enhance the stability of catalysts. However, excessive K can serve as weak acid sites, poison of active sites and inhibitor of H 2 adsorption on Pt surface, leading to deactivation of the catalyst.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yulin Yao,Zonghan Yang,Pengfei Zheng,Zhenheng Diao
The accessible TiO 6 concentration and the hydrophobicity of hierarchical TS-1 zeolites are both considered as critical factors for promoting the catalytic performance of selective oxidation. However, simultaneously enhancing these two factors still remains a challenge. Herein, hierarchical TS-1 zeolites with high hydrophobicity and concentration of accessible TiO 6 were fabricated via sequential amphiphilic organosilane-assisted pre-treatment, dry-gel conversion and hydroxyl radical post-treatment, using amphiphilic organosilane and surfactant as meso -porogens. The synthesis mechanism was investigated, and it was found that the interaction between the amphiphilic organosilane and Ti species formed during the pre-treatment benefited the formation of accessible TiO 6 during the subsequent dry-gel conversion. During the post-treatment of zeolite with H 2 O 2 solution under ultraviolet light irradiation, appropriate H 2 O 2 concentration facilitated the generation of hydroxyl radical and thus enhanced the hydrophobicity of zeolite without significantly influencing the TiO 6 concentration. The hierarchical TS-1 displayed excellent catalytic performances in 1-hexene epoxidation and oxidative desulfurization, with conversion improvements of 91.2%±2.8% and 348%±17%, respectively, compared with the conventional counterpart.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）