Nutrients
·
2024
·Vol. 16
·期 5
·600
·
IF 4.8
Juan Yang,He Gao,Tiantian Zhang,Yong Fan,Yuwei Wu,Xinyu Zhao,Ying Li,Lei Wu,Hui Zhao,Lingshuang Yang,Haojie Zhong,Longyan Li,Xinqiang Xie,Qingping Wu
Hepatocellular carcinoma (HCC), being ranked as the top fifth most prevalent cancer globally, poses a significant health challenge, with a considerable mortality rate. Hepatitis B virus (HBV) infection stands as the primary factor contributing to HCC, presenting substantial challenges in its treatment. This study aimed to identify lactic acid bacteria (LAB) with anti-HBV properties and evaluate their impact on the intestinal flora in HBV-associated HCC. Initially, two LAB strains,Levilactobacillus brevisSR52-2 (L. brevisSR52-2) andLeviLactobacillus delbrueckiisubsp.bulgaicusQ80 (L. delbrueckiiQ80), exhibiting anti-HBV effects, were screened in vitro from a pool of 498 LAB strains through cell experiments, with extracellular expression levels of 0.58 ± 0.05 and 0.65 ± 0.03, respectively. These strains exhibited the capability of inhibiting the expression of HBeAg and HBsAg. Subsequent in vitro fermentation, conducted under simulated anaerobic conditions mimicking the colon environment, revealed a decrease in pH levels in both the health control (HC) and HCC groups influenced by LAB, with a more pronounced effect observed in the HC group. Additionally, the density of total short-chain fatty acids (SCFAs) significantly increased (p< 0.05) in the HCC group. Analysis of 16S rRNA highlighted differences in the gut microbiota (GM) community structure in cultures treated withL. brevisSR52-2 andL. delbrueckiiQ80. Fecal microflora in normal samples exhibited greater diversity compared to HBV-HCC samples. The HCC group treated with LAB showed a significant increase in the abundance of the phyla Firmicutes, Bacteroidetes and Actinobacteria, while Proteobacteria significantly decreased compared to the untreated HCC group after 48 h. In conclusion, the findings indicate that LAB, specificallyL. brevisSR52-2 andL. delbrueckiiQ80, possessing antiviral properties, contribute to an improvement in gastrointestinal health.
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Cureus Journal of Medical Science
·
2024
·
IF 1
Ye-Ran Mao,Ling-Yi Zhu,Ruo-Fei Du,Xiao-Yu Liu,Zhidan Liu,Li Li
Background and objectives: In ancient China, bee venom was widely used to treat various diseases. Although using bee venom is not currently a mainstream medical method, some have applied it to treat certain conditions, including idiopathic facial paralysis (IFP). Recently, melittin (Mel), the main active component of bee venom, has been shown strong anti-inflammatory and analgesic effects. However, how bee venom improves neurological dysfunction in facial paralysis remains unknown. This study aimed to investigate the anti-neurotraumatic effect of Mel on Schwann cells (SCs), the main cells of the neuron sheath, injured by oxidative stress. Methods: A model of hypoxic SCs was established, and CCK-8 assay, siRNA transfection, enzyme-linked immunosorbent assay, quantitative reverse transcription-polymerase chain reaction, western blot, immunofluorescence, and cell ultrastructure analyses were conducted to investigate the mitigation of hypoxia-induced damage to SCs in vitro, revealing the effects of Mel on oxidative stress injury in SCs. Results: The overexpression of HIF-1α in CoCl 2 -induced SCs (p < 0.05) indicated the establishment of an SCs hypoxia model. The proliferation and regeneration process of the hypoxic SCs enhanced in the Mel-treated group compared to the CoCl 2 group has been proven through the CCK-8 experiment (p < 0.0001) and S-100 mRNA expression detection (p < 0.0001). The increased level of reactive oxygen species (ROS) (p < 0.001) and decreased superoxide dismutase (SOD) levels (p < 0.05) in the CoCl 2 -induced SCs indicated that Mel can alleviate the oxidative stress damage to SCs induced by CoCl 2 . Mel alleviated oxidative stress and inflammation in hypoxic SCs by reducing pro-inflammatory cytokines IL-1β (p < 0.0001) and TNF-α (p < 0.0001). In addition, Mel augmented cellular vitality and regulated indicators related to oxygen metabolism, cell repair, neurometabolism, and vascular endothelial formation after hypoxia, such as C-JUN (p < 0.05), glial cell line-derived neurotrophic factor (GDNF; p < 0.001), vascular endothelial growth factor (VEGF; p < 0.05), hypoxia-inducible factor 1-alpha (HIF-1α; p < 0.05), interleukin-1 receptor type 1 (IL-1R1; p < 0.05), enolase1 (ENO1; p < 0.05), aldose reductase (AR; p < 0.01), SOD (p < 0.05), nerve growth factor (NGF; p < 0.05), and inducible nitric oxide synthase (iNOS; p < 0.05). In terms of its mechanism, Mel inhibited the expression of proteins associated with the NF-κB pathway such as IKK (p < 0.01), p65 (p < 0.05), p60 (p < 0.001), IRAK1 (p < 0.05), and increased IKB-α (p < 0.0001). Moreover, knocking out of IL-1R1 in the si-IL-1R1 group enhanced the therapeutic effect of Mel compared to the Mel-treated group (all of which p < 0.05). Conclusion: This research provided evidence of the substantial involvement of IL-1R1 in oxidative stress damage caused by hypoxia in SCs and proved that Mel alleviated oxidative stress injury in SCs by targeting IL-1R1 to downregulate the NF-κB-mediated inflammatory response. Mel could potentially serve as an innovative therapeutic approach for the treatment of IFP.
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LANGMUIR
·
2024
·
IF 3.7
Adugna Boke Abdeta,Feyisa Wedajo,Qinhan Wu,Dong-Hau Kuo,Ping Li,Hanya Zhang,Ting Huang,Jinguo Lin,Xiaoyun Chen
Cellulose plays a significant role in designing efficient and stable cellulose-based metallic catalysts, owing to its surface functionalities. Its hydroxyl groups are used as anchor sites for the nucleation and growth of metallic nanoparticles and, as a result, improve the stability and catalytic activity. Meanwhile, cellulose is also amenable to surface modifications to be more suitable for incorporating and stabilizing metallic nanoparticles. Herein, the Ag-/Bi-doped Mo(S,O)3 trimetallic sulfo-oxide anchored on B and N codoped cellulose (B–N–C) synthesized by a facile approach showed excellent stability and catalytic activity for PHER at 573.28 μmol/h H2 with 25 mg of catalyst under visible light, and 92.3% of the 4-nitrophenol (4-NP) reduction was achieved within 135 min by in situ-generated protons. In addition to B and N codoping, our use of the calcination method for B–N–C preparation further increases the structural disorders and defects, which act as anchoring sites for Ag-/Bi-doped Mo(S,O)3 nanoparticles. The Ag-/Bi-doped Mo(S,O)3@B–N–C surface active site also stimulates H2O molecule adsorption and activation kinetics and reduces the photogenerated charge carrier’s recombination rate. The Mo4+ → Mo6+ electron hopping transport and the O 2p and Bi 6s orbital overlap facilitate the fast electron transfer by enhancing the electron’s lifetime and photoinduced charge carrier mobility, respectively. In addition to acting as a support, B–N–C provides a highly conductive network that enhances charge transport, and the relocated electron in B–N–C activates the H2O molecule, which enables Ag-/Bi-doped Mo(S,O)3@B–N–C to have appreciable PHER performance.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Jinghang Xu,Zebin Zhuang,Jiahui Yu,Yingping Li,Lulu Cheng,Ruixiang Peng,Jun Shi
In order to construct a reactive oxygen species (ROS) hydrogel loaded with Sodium Tanshinone IIA Sulfonate (STS) and Salvianolic Acid B (SAB) and to study its biological effects on scar fibroblasts and anti-scar efficacy, a ROS-responsive hydrogel (HA-PBA-PVA@STS/SAB) was prepared by combining hyaluronic acid (HA), phenylboronic acid (PBA) and polyvinyl alcohol (PVA). The ROS-responsive drug release behavior of the hydrogels was examined, and cell proliferation activity was determined using the CCK-8 method. Cell migration capability was analyzed through scratch assays and Transwell invasion assays, while flow cytometry was employed to quantify apoptosis rates. The intracellular ROS levels were measured. Gene expressions of NOX4, COL-I, COL-III, and p38 were detected via qRT-PCR, with Western blot analysis confirming the expression levels of NOX4 and p38 proteins. Finally, the in vivo anti-scar efficacy of the hydrogel was evaluated. The results showed that HA-PBA-PVA@STS/SAB hydrogels exhibited responsive drug release characteristics to ROS. Compared with the model group, the ROS content in scar fibroblasts was significantly reduced ( p <0.001), the cell proliferation, migration and invasion ability were significantly decreased, and the apoptosis rate was significantly increased ( p <0.001). In addition, the expression levels of NOX4 and p38 proteins in cells were also significantly reduced ( p <0.05). The in vivo pharmacodynamic study demonstrated that, compared to the model group, the HA-PBA-PVA@STS/SAB hydrogel significantly reduced scar thickness, decreased the number of fibroblasts and collagen fibers, and downregulated the expression levels of COL-I and α-SMA proteins ( p <0.05). These findings demonstrate that the HA-PBA-PVA@STS/SAB hydrogel possesses remarkable ROS-responsive drug release capability, anti-scar efficacy, and promising potential for scar treatment.
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JOURNAL OF ALLOYS AND COMPOUNDS
·
2025
·
IF 6.3
Sihang Ji,Lijia Zhao,Chen Chen,Jialong Zhao,Jin Wang,Jinju Zheng,Xi Yuan
The development of quasi-two-dimensional (quasi-2D) perovskite films with high photoluminescence quantum yield (PL QY) and robust environmental stability remains a critical challenge for advanced optoelectronic applications. Herein, we report an A/B-site synergistic doping strategy by incorporating multivalent Cs + (A-site) and Cd 2+ (B-site) ions into quasi-2D BA 2 MA n-1 Pb n Br 3n+1 films, significantly enhancing their optical quality and structural stability. Notably, the bimetallic ion-doped films achieve a high PL QY of up to 90 % at 490 nm, along with excellent homogeneous phase stability (the phase separation of quasi-2D films emitting at 435 nm and 490 nm is suppressed even after 1 h and 10 h). Moreover, co-doping with multivalent Cs + and Cd 2+ ions induces a significant reduction in both grain size (10–15 nm) and surface roughness (∼1 nm), while endowing the quasi-2D films with a high exciton binding energy (>300 meV). The variable temperature PL spectra reveal the band-edge radiative dynamics, which originate from the thermal competition between exciton and trap-mediated recombination. The heat-induced electron-phonon interaction enhances the proportion of trap-mediated recombination, leading to an increase in the average PL lifetime. The A/B-site synergistic engineering provides a novel approach for improving the PL performance and photostability of quasi-2D perovskite films, paving the way toward high-performance light-emitting diodes, lasers, and other ptoelectronic devices based on the luminescent layer.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Na Xiao,Shi Gang Liu,Shi Mo,Yu Zhu Yang,Lei Han,Yan Jun Ju,Nian Bing Li,Hong Qun Luo
In this work, a two-dimensional optical sensing platform for H 2 O 2 , glucose, and uric acid (UA) detection is developed, which integrates the advantages of colorimetric and ratiometric fluorescent techniques. ZnFe 2 O 4 magnetic microspheres with an intrinsic peroxidase-like activity are synthesized to catalyze the oxidation of o-phenylenediamine in the presence of H 2 O 2 , producing a typical yellow substance (oxOPD) with an absorption peak at 420 nm. The oxOPD can significantly quench the fluorescence of boron and nitrogen co-doped CDs (B,N-CDs) at 430 nm through the inner filter effect and generate a new fluorescence emission peak at 556 nm. Thus, the fluorescence intensity ratio ( I 556 / I 430 ) can be utilized for quantitative analysis of the concentrations of H 2 O 2 and H 2 O 2 -involved metabolites (glucose and UA). The colorimetric “naked-eye” readout based on the color change of solution can also be established to determine H 2 O 2, glucose, and UA levels. The detection limit based on colorimetric sensing for H 2 O 2, glucose, and UA are 0.09, 0.9, and 0.9 μM, respectively, and 0.1, 8, and 1 μM using ratiometric fluorescent sensing. Furthermore, this strategy can detect glucose and UA in human serum with satisfactory results, and provide potential applications in the detection of metabolites related to H 2 O 2 release.
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JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2014
·Vol. 52
·期 22
·3205-3212
·
IF 3.245
Zhixian Xu,Suyun Jie,Bo-Geng Li
Well-defined diblock copolymers of linear polyethylene (PE) and poly(dimethylsiloxane) (PDMS) have been synthesized through a facile route combining the thiol-ene click chemistry of vinyl-terminated polyethylene (PE-ene) and the sequential esterification reaction. The resulting diblock copolymers are characterized by 1 H NMR, FT-IR, DSC, TGA, and TEM. In addition, the PE- b -PDMS diblock copolymers have been evaluated as compatibilizers in the blends of high-density polyethylene (HDPE) and silicone oil. The morphological analysis and mechanical properties demonstrate that the compatibilized blends with low loading concentration of PE- b -PDMS display significant improvements in modulus of elasticity and elongation at break as compared to the uncompatibilized binary blends. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014 , 52 , 3205–3212
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EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2021
·Vol. 248
·期 2
·553-565
·
IF 2.998
Wang Liling,Qin Yuchuan,Wang Yanbin,Liu Bentong,Zhou Yifeng
In this research, the inhibition mechanism of a pairs of homoisoflavonoid analogues (6-aldehydo-isoophiopogonanone A (AIO-A) and 6-aldehydo-isoophiopogonanone B (AIO-B)) on tyrosinase (Tyr) was investigated by multi-spectroscopic techniques combined with molecular docking. The results demonstrated that AIO-A suppressed the activity of Tyr in a reversible competitive-inhibition with a half inhibitory concentration (IC50) of (12.67 ± 2.52) × 10 –5 molˑL −1 , while AIO-B was a reversible mixed-inhibition with IC50 of (4.81 ± 0.74) × 10 –5 molˑL −1 . AIO-A/AIO-B bound to Tyr driven by the intermolecular interactions, including van der Waals interaction, hydrogen bonding and hydrophobic interaction, thereby resulted in a secondary structure change of Tyr and its intrinsic fluorescence quenching. The molecular docking revealed that an aldehyde oxygen atom in AIO-A and methoxyphenyl in AIO-B formed coordination with two Cu ions in the active center of Tyr protein. It can be deduced that the coordination with two Cu ions may prevent the entrance of substrate and then inhibit the catalytic activity of Tyr. The research might provide new perspectives on the inhibition mechanism of AIO-A and AIO-B on Tyr and functional research for the prevention and treatment of pigmented skin diseases and anti-browning of homoisoflavonoids as a food supplement.
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Journal of Environmental Chemical Engineering
·
2021
·
IF 5.909
Linjuan Pei,Xianmo Gu,Yantao Zhang,Jie Wang,Hao Tan,Pengfei Wang,Zhanfeng Zheng
To improve the photocatalytic performance of TiO 2 and make it easier to recycle, the titanosilicate@anatase core-shell micro-nanostructure photocatalysts were designed. The titanosilicate@anatase core-shell micro-nanostructure was fabricated by a two-step method: an acid-assisted hydrothermal treatment of the hydrogen-form natisite and a subsequent calcination . In the first step, the dissolution and recrystallization of Ti species led to the formation of TiO 2 shell over the titanosilicate; in the second step, the calcination not only facilitated the formation of Ti−O−Si bond, but also improved the crystallization of TiO 2 shell. The rutile TiO 2 was not detected even at 900 °C, which could be attributed to the strong interaction between TiO 2 nanocrystals and the titanosilicate core through Ti−O−Si bond at the interface. The formation of Ti−O−Si bond also changed the surface characteristic of the TiO 2 shell, which facilitated the enrichment of RhB dye molecules around the catalyst and induced the fast production of the O 2 •− . The collaboration of the core and the shell contributed to the quick degradation of RhB under visible light and the excellent recycling performance. The growth kinetics of the core-shell micro-nanostructure has been studied in detail, which provided a novel and facile synthesis strategy for the fabrication of TiO 2 micro-nanostructure easy to be recycled.
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ACTA CHIMICA SLOVENICA
·
2017
·Vol. 64
·期 4
·825-831
·
IF 0.983
Ren YJ,Zhu JL,Zhang LX,Xu YX,Qian SS.
Three new complexes derived from 2-(4-(pyridin-2-yl)piperazin-1-yl)acetic acid (HL), [M(L)2(H2O)2] where M = CuII (1), ZnII (2) and CdII (3), have been synthesized and characterized by IR spectroscopy, elemental analysis and X-ray crystallography. The inhibitory activity of these three complexes against MAO-B was tested in vitro, and the molecular docking experiments were also carried out to rationalize their binding models. Both the experimental and docking simulation results indicated that complex 1 has the best inhibitory activity with IC50 value being 6.5 ± 0.31 μM.
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ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Dishi Wang,Yanxia Zhou,Xinru Li,Xiaoyou Qu,Yunqiang Deng,Ziqi Wang,Chuyu He,Yang Zou,Yiguang Jin,Yan Liu
pH-responsive polymeric micelles have shown promise for the targeted and intracellular delivery of antitumor agents. The present study aimed to elucidate the possible mechanisms of pH-sensitivity and cellular internalization of PEOz-b-PLA micelles in detail, further unravel the effect of hydrophilic/hydrophobic ratio of the micelles on their cellular internalization, and examine the intracellular trafficking routes and fate of PEOz-b-PLA after internalization of the micelles. The results of variations in the size and Zeta potential of PEOz-b-PLA micelles and cross-sectional area of PEOz-b-PLA molecules with pH values suggested that electrostatic repulsion between PEOz chains resulting from ionization of the tertiary amide groups along PEOz chain at pH lower than its pKa was responsible for pH-sensitivity of PEOz-b-PLA micelles. Furthermore, the studies on internalization of PEOz-b-PLA micelles by MCF-7 cells revealed that the uptake of PEOz-b-PLA micelles was strongly influenced by their structural features, and showed that PEOz-b-PLA micelles with hydrophilic/hydrophobic ratio of 1.7–2.0 exhibited optimal cellular uptake. No evident alteration in cellular uptake of PEOz-b-PLA micelles was detected by flow cytometry upon the existence of EIPA and chlorpromazine. However, the intracellular uptake of the micelles in the presence of MβCD and genistein was effectively inhibited. Hence, the internalization of such micelles by MCF-7 cells appeared to proceed mainly through caveolae/lipid raft-mediated endocytosis without being influenced by their hydrophilic/hydrophobic ratio. Confocal micrographs revealed that late endosomes, mitochondria and endoplasmic reticulum were all involved in the intracellular trafficking of PEOz-b-PLA copolymers following their internalization via endocytosis, and then part of them was excreted from tumor cells to extracellular medium. These findings provided valuable information for developing desired PEOz-b-PLA micelles to improve their therapeutic efficacy and reducing the potential safety risks associated with their intracellular accumulation.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2020
·
IF 4.939
Wenyu He,Jinhui Wei,Ting Huang,Enren Zhang
For practical applications of microbial fuel cells (MFCs), 5H-dibenz [b,f] azepine-5-carboxamide (Carbamazepine, CBZ) was used as the organic precursor for the synthesis of catalysts composed of Fe–N–C complexes (named as Fe-CBZ-Cats) through a sacrificial support method. Characterizations by SEM, XPS and TEM-EDS elemental mapping show that the synthesized Fe-CBZ-Cats have porous structure and uniformly dispersed Fe/N/C complex centers which could play key roles in effective ORR . Linear scanning voltammetry (LSV) measurements show that the synthesized Fe-CBZ-Cats exhibits ORR activity comparable to commercial Pt/C catalysts under the same catalyst loading (2 mg cm −2 ). The maximum power density produced by cathode-limiting MFCs with Fe-CBZ-Cats air cathodes was 431 ± 23 μW cm −2 , higher than those in the control MFCs with Pt/C air cathodes (403 ± 13 μW cm −2 ) and activated carbon air cathodes (296 ± 24 μW cm −2 ). In addition, the toxicity tolerance of the synthesized Fe-CBZ-Cats is much higher than Pt/C.
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