Journal of Neurorestoratology
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2025
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IF 3.4
Dapeng Zhao,Hengrui Li,Xiaoyu Wang,Ailing Yu,Lanhua Li,Baoliang Sun,Ying Wang,Guojun Wang,Jingyi Sun
Background Spinal cord injury (SCI), which often results from traumatic incidents, leads to neural damage and impaired sensory and motor functions and may pose a serious threat to life. Secondary injury mechanisms caused by persistent inflammation disrupt the local microenvironment, causing neuronal cell death and hindering neural regeneration. This study used a chitosan-citric acid (CS-CA) hydrogel as a carrier for Catalpol (CAT-CS-CA), which was directly applied to the injury site to promote SCI repair. Methods CAT-CS-CA and CS-CA hydrogels were characterized and implanted into rat SCI models. 24 male Sprague-Dawley rats (230–250 g) were randomly assigned to four groups ( n = 6 per group) using a randomization principle: sham operation group (laminectomy only), SCI group, CS-CA hydrogel group, and CAT-CS-CA hydrogel group. Neuronal nuclei (NeuN), β III-tubulin (Tuj1), glial fibrillary acidic protein (GFAP), macrophage polarization (M1/M2 markers), and hindlimb function (BBB score) were assessed. Results The CAT-CS-CA hydrogel retains CS-CA hydrogel's advantages and gains enhanced neuroprotective and anti-inflammatory abilities. The implantation of CAT-CS-CA into injured rat spinal cords enhanced neuronal survival, stimulated axonal regeneration, and significantly suppressed glial proliferation at the injury site. In addition, it promoted macrophage polarization to the M2 phenotype and substantially enhanced hindlimb motor function in rats with SCI. Conclusion CAT-CS-CA hydrogel promotes neuronal survival, suppresses glial scarring, and improves motor function, offering a promising strategy for SCI repair.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Jiahao Kang,Qiangli Zhao,Min Mao,Yugen Wang,Qiang Mu,Jie Tao,Xiaoliang Zhao,Xinhai He,Jianwei Li
Cellulose-based triboelectric materials present eco-friendly advantages but suffer from restricted electron transfer and humidity-induced charge loss due to inherent hydrogen-bond networks. Herein, a multi-network composite aerogel was fabricated using multi-scale entangled cellulose and regenerated chitosan fibers as a framework. Molecular-level chemical crosslinking, ionic complexation, and hydrogen bonding were integrated with freeze-induced structuring, solvent exchange, rapid desolvation, and ambient drying. The multi-crosslinked network delivers exceptional mechanical robustness (0.37 MPa stress at 50% strain), resilience, and moisture stability (intact structure after 40-day immersion). The aerogel-based triboelectric nanogenerator (TENG) demonstrated high triboelectric outputs (205.8 V, 581.16 mW/m 2 ), fatigue resistance (5000 cycles), humidity responsiveness (70–90% RH), broad humidity adaptability (40–90% RH), wet-environment durability (6800 cycles at 80% RH), and reversible humidity cycling, alongside stable acoustic/electrical signal recognition. The aerogel exhibited recyclability and biodegradability. This work establishes a green manufacturing paradigm for biomass aerogels, bridging sustainable materials engineering with self-powered environmental sensing technologies.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Yidan Liu,Xin Luo,Wei Wu,Andi Zhang,Bingchuan Lu,Ting Zhang,Ming Kong
In situ 3D printing technologies is a new frontier for highly personalized medicine, which requires suitable bioink with rheology, biocompatibility, and gelation kinetics to support the right shape and mechanical properties of the printed construct. To this end, a facile design of thermo/photo dual cure composite hydrogel was proposed using MHBC and soluble collagen in this study. M/C composite hydrogel exhibited rapid thermo-induced sol-gel transition and contraction, tunable mechanical properties, proper microstructure and biodegradability for 3D cell culture, as well as improve cyto-compatibility, all of which were dependent upon the methacrylation degree of MHBC and M/C ratios. The printability of the optimal formulation (3% MHBC/1% collagen) was validated by its mild printing condition, rapid gelation of bioink at 37 °C and simple postprocessing manipulation. Both desirable printability and cyto-compatibility enable M/C composite hydrogel a potential candidate as bioink to be applied for in situ 3D bioprinting.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2020
·Vol. 21
·期 5
·1635
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IF 4.556
Yanan Wang,Shangyong Li,Mengfei Jin,Qi Han,Songshen Liu,Xuehong Chen,Yantao Han
The recent emergence of antibiotic-resistant bacteria requires the development of new antibiotics or new agents capable of enhancing antibiotic activity. Lysozyme degrades bacterial cell wall without involving antibiotic resistance and has become a new antibacterial strategy. However, direct use of native, active proteins in clinical settings is not practical as it is fragile under various conditions. In this study, lysozyme was integrated into chitosan nanoparticles (CS-NPs) by the ionic gelation technique to obtain lysozyme immobilized chitosan nanoparticles (Lys-CS-NPs) and then characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM), which showed a small particle size (243.1 ± 2.1 nm) and positive zeta potential (22.8 ± 0.2 mV). The immobilization significantly enhanced the thermal stability and reusability of lysozyme. In addition, compared with free lysozyme, Lys-CS-NPs exhibited superb antibacterial properties according to the results of killing kinetics in vitro and measurement of the minimum inhibitory concentration (MIC) of CS-NPs and Lys-CS-NPs againstPseudomonas aeruginosa(P. aeruginosa),Klebsiella pneumoniae(K. pneumoniae),Escherichia coli(E. coli), andStaphylococcus aureus(S. aureus). These results suggest that the integration of lysozyme into CS-NPs will create opportunities for the further potential applications of lysozyme as an anti-bacterium agent.Keywords:immobilization;chitosan nanoparticles;lysozyme;anti-bacterium activity
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
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IF 4.784
Yingbei Huang,Fulin Shi,Liming Wang,Yu Yang,Bilal Muhammad Khan,Kit-Leong Cheong,Yang Liu
This study aims at developing an effective, safe and economic hydrogel, with wound healing ability, by making use of polysaccharides. Bletilla striata polysaccharide (Bsp) and the bioactive natural polymers, carboxymethyl chitosan (CMC), were physically blended with Carbomer 940 (CBM 940 ) in order to promote gel-forming and improve water retention. FT-IR displayed hydrogen bonding between CBM 940 and CMC played a role in physical gel formation. XRD showed broad and weak intensity peak of Bsp/CMC/CBM 940 hydrogel. SEM, rheological measurement and water loss test revealed that the best hydrogel with optimum characteristics in relation to porous structure, elastic property and water retention could be obtained by mixing Bsp, CMC and CBM 940 in a ratio of 4:1:1. In vitro studies proved that the Bsp/CMC/CBM 940 hydrogel possessed blood compatibility. M293T cells viability was over 85% via MTT assay which revealed non-cytotoxicity. Hydroxyl radical scavenging property highly improved while Bsp combined with CMC. The full-thickness wound experiment illustrated 71.64 ± 6.64% and 83.80 ± 5.56% wound healing rates for Bsp4:CMC1:CBM 940 1 hydrogel on Day 7 and Day 14, respectively, which showed no significant differences (p > 0.05) in comparison to the positive control. Histological observation expressed epithelization, dense collagen fiber and neovascular formation in hydrogel group on Day 14. Bsp/CMC/CBM 940 hydrogel can, hence, serve as an attractive candidate for healing wounds.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Peng Jiang,Lijie Huang,Junjie Wang,Qiulei Li,Haibo Mu
Wound infection especially that induced by drug resistant bacteria has been considered an increasing medical crisis. Herein a biocompatible wound dressing is conveniently constructed by incorporating (Sr 0.6 Bi 0.305 ) 2 Bi 2 O 7 (denoted as SBO) with excellent photothermal performance into a facile antibacterial hydrogel (gel) obtained from multiple physical crosslinks among Ag + , carboxymethyl chitosan and polyacrylic acid. The prepared SBO gel features excellent bactericidal activities, hemostasis, adequate mechanical properties, adhesiveness and adsorption capacities to bacterial cells and toxin. The gel can disperse SBO homogeneously in the network and SBO effectively convert visible light energy into localized heat for synergistic sterilization. In vitro assays confirm the potent broad-spectrum bactericidal activities of SBO gel to some common pathogens and drug resistant strains such as MRSA and CAPA. Mice model of MRSA-induced wound infections verified the practical efficacy of SBO gel in combating bacterial infections and accelerating wound healing. Moreover, this is the first report of SBO as a photothermal agent applied in anti-infection treatment. All of these results highlight the potential application of SBO gel in drug-resistant bacteria associated wound management.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Saisai Gu,Jingya Xu,Wangtianzi Teng,Xiao Huang,Hao Mei,Xinting Chen,Gang Nie,Zheng Cui,Xiqiu Liu,Yu Zhang,Kaiping Wang
Postsurgical localized chemotherapy for breast cancer recurrence (BCR) still faces many problems which dampen researchers' enthusiasm and discounted prognosis. Simple strategies with controllable toxicities are expected to address these hurdles. Lentinan (LNT) has excellent biocompatibility and notable antitumor activity but rather low bioavailability after intravenous or oral administration. Here, a sponge-like LNT/chitosan composite (LNT/CS sponge) was prepared for efficient local delivery to prevent postoperative BCR. The obtained sponges exhibit uniform porosity and sustained release of LNT in vitro and in vivo. Furthermore, the sponges were implanted and showed significant reduction of postsurgical recurrence and suppression of long-term tumor regrowth with favorable biocompatibility in a subcutaneous postsurgical recurrence mouse model. Subsequent studies revealed that LNT can restrain the stemness of breast cancer cells, which may account for the long-term inhibition of tumor relapse. Therefore, LNT/CS sponge has a great potential as a promising alternative for postsurgical BCR.
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International Journal of Nanomedicine
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2023
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IF 8
Li Lin,Junyi He,Jiawei Li,Yan Xu,Jingxia Li,Yangzhe Wu
Background During the past few years, immune cell therapy for malignant cancer has benefited a considerable amount of patients worldwide. As one of several promising candidates for immunotherapy, Vγ9Vδ2 γδ T cells have many unique biological advantages, such as non-MHC restriction and have been noted as the earliest source of IFN-γ. However, potentiating anti-tumor functions of γδ T cells has become of particular interest to researchers studying γδ T cell applications.Purpose In this study, we proposed a nanotechnology-based methodology for strengthening γδ T cell functions.Methods As a type of reliable, biocompatible material, chitosan nanoparticles (CSNPs) were used to enhance anti-tumor immunity of γδ T cells.Results First, we found that the size of prepared CSNPs distributed 50 to 100 nm, and that CSNPs had optimal immunocompatibility. Then, we observed that CSNPs could induce α-tubulin cytoskeleton polarization and rearrangement, correlating with a higher killing ability of γδ T cells. Furthermore, we revealed that CSNPs could enhance Vγ9Vδ2 T cell anti-tumor functions by upregulating killing of related receptors, including NKG2D, CD56, FasL, and perforin secretion.Conclusion Our work provided evidence of application for CSNPs based bio-carrier in immunotherapy. More importantly, we proposed a new strategy for enhancing γδ T cell anti-tumor activity using nanobiomaterial, which could benefit future clinical applications of γδ T cells.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xiajun Liang,Fenbo Ma,Yuhui Chen,Xiaochun Liu,Chaoqun Yu,Guoyun Cheng,Dawei Sun,Bin Tang,Chenghe Qin,Lei Xu
Wound healing continues to pose significant challenges in clinical practice, with hydrogels often limited by their fragility, inadequate adhesion, and restricted biological functionalities. To address these issues, a composite hydrogel, consisting of polyvinyl alcohol (PVA), bacterial cellulose (BC), and chitosan oligosaccharide (COS), termed PBC hydrogel, was developed. An evaluation of their mechanical properties, including tensile and compressive strength, was conducted. Notably, the incorporation of BC enhanced these mechanical attributes of the PBC hydrogels. Furthermore, the self-healing and adhesive properties of the PBC hydrogels were assessed, revealing excellent performance in both aspects. In vitro cellular assessments were carried out to evaluate the biological effects of the PBC hydrogels. The results indicated that the PBC hydrogel can effectively promote the proliferation and migration of fibroblasts, as well as upregulate the expression of connective tissue growth factor (CTGF) and downregulate the expression levels of inflammatory cytokines interleukin-1β (IL-1β) and interleukin-6 (IL-6). It is imperative to note that preliminary in vivo results have further validated the effectiveness of the PBC hydrogel in promoting wound healing. This finding provides substantial support for the application of the PBC hydrogel in wound healing, demonstrating its therapeutic potential in promoting wound healing.
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ACS Applied Materials & Interfaces
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2025
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IF 8.2
Kangjie Zhou,Yong Liu,Zhuo Huang,Zhengping Fang,Hai Liu,Zushun Xu,Ning Zhao
Biomass aerogels, as low-density, high-porosity, and renewable materials, have attracted significant attention in recent years. Compared with vacuum drying and supercritical drying, ambient drying has potential for the large-scale production of aerogels but still faces challenges such as structural collapse. In this work, chitosan, a widely available polysaccharide, was employed to construct a lightweight, high-strength aerogel using an improved freeze-drying method. This strategy involves prefreezing to control the high-density cross-linking of chitosan, thereby reinforcing the wet gel skeleton and mitigating capillary-induced destructive collapse during subsequent ambient drying. The resulting aerogel features a robust porous architecture, delivering an exceptional mechanical strength of 0.76 MPa, outstanding compressive fatigue resistance, and low thermal conductivity of 46 mW/m·K at reduced density. In addition, the good hydrophobic properties of this aerogel were endowed by chemical vapor deposition and interfacial modification. Impressively, the obtained aerogel was further demonstrated to possess significant advantages in multifunctional applications such as impact resistance, thermal insulation, infrared stealth, and efficient oily wastewater treatment. We believe that the proposed improved freeze-drying method provides a simple yet effective approach for preparing high-performance biomass aerogels suitable for various practical applications.
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FOOD CHEMISTRY
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2025
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IF 9.8
Shupeng Su,Junhua Lu,Xinyi Yin,Mengjiao Wang,Fumin Wang,Yuejiu Pang
β-carotene, a vital carotenoid, is limited by poor solubility and stability. Though Pickering nanoemulsions offer superior protection for β-carotene than conventional nanoemulsions, developing food-grade Pickering nanoemulsions remains challenging due to particle size and amphiphilicity issues. This study developed a food-grade Pickering nanoemulsion stabilized by chitosan nanoparticles via high-power ultrasonication. Under optimized conditions (pH 6.5, 2 % oil phase, 1.0 wt% chitosan, 455 W ultrasonication for 180 min), a stable nanoemulsion of 388 nm was obtained with minimized β-carotene loss. These nanoemulsions exhibited exceptional environmental stability, maintaining robust stability under varying pH (3.0–7.0), ionic strength (100–500 mM NaCl), and thermal stress. Thermal stability and extended storage tests demonstrated superior β-carotene protection compared to conventional Tween 20-stabilized emulsions. This work provides a novel strategy for food-grade Pickering nanoemulsions, enabling β-carotene application in functional foods and pharmaceuticals.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Tao Sheng,Shuai Xia,Jia-Min Li,Ruo-bin Peng,Pingbo Yan,Bing-Feng Zhang
The activation and inflammation of hepatic stellate cells (HSCs) promoted the progression of liver fibrosis. The antioxidant and anti-inflammatory therapy can effectively alleviate liver fibrosis. Here, dual prodrug nanoassemblies modified with chitosan (CS/HS-HD NPs) was constructed. The particle size of CS/HS-HD NPs was about 200 nm, which was uniformly dispersed spherical particles, and Zeta potential was positive. The CS/HS-HD NP loaded SLB 3.66 ± 0.04 % and DEX 0.52 ± 0.01 %. In vitro stability and release investigation showed CS/HS-HD NPs had good stability and sustained release characteristics without burst release. These results of in vivo pharmacokinetics, intestinal absorption and in vivo distribution showed that CS/HS-HD NPs had longer t l/2 and larger AUC than other groups (Free drug, HS-HD NPs), which significantly enhanced intestinal absorption and specific accumulation in the liver. In CCl 4 -induced liver fibrosis, CS/HS-HD NPs significantly inhibited the secretion of pro-inflammatory cytokines TNF-α, IL-6 and IL-12, and increased anti-inflammatory cytokines IL-10 level, and then reduce the infiltration of inflammatory cells. The image of liver, HE staining, Masson staining showed CS/HS-HD NPs reduced collagen deposition and liver pathological damage, effectively improved liver fibrosis. Taken together, these findings suggested chitosan coated hybrid prodrug-nanoassemblies could adjust inflammatory cytokines and improve therapeutic effect on liver fibrosis.
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