JOURNAL OF CELLULAR PHYSIOLOGY
·
2020
·Vol. 236
·期 3
·1889-1902
·
IF 5.546
Wenhui Yao,Kai Wang,Xiniao Wang,Xinran Li,Jieyan Dong,Yusheng Zhang,Xuansheng Ding
Icariin (ICA), as a flavonoid glycoside, is associated with the improvement of vascular complications in diabetes. However, its protective mechanisms remain to be well-established. Here, we tested the hypothesis that ICA attenuates vascular endothelial dysfunction by inhibiting endoplasmic reticulum (ER) stress in type 1 diabetes. In streptozotocin-induced diabetic rats, ICA positively affected acetylcholine-induced vasodilation and phenylephrine-induced vasoconstriction in aortas. ICA treatment significantly attenuated ER stress in diabetic rats and high-glucose induced human umbilical vein endothelial cells. Incubation with ICA in vitro attenuated vascular reactivity in diabetic rats, which was blocked by the ER stress inducer, and peroxisome proliferator-activated receptor α (PPARα), sirtuin1 (Sirt1), or AMP-activated protein kinase-α (AMPKα) inhibitors. Western blot showed that ICA activated the PPARα/Sirt1/AMPKα pathway, which contributed to reducing ER stress and activating endothelial nitric oxide synthase in vivo and vitro. Our results implicate that ICA normalizes ER stress to attenuate endothelial dysfunction by the regulation of the PPARα/Sirt1/AMPKα pathway.
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Catalysis Science & Technology
·
2019
·Vol. 9
·期 24
·7034-7044
·
IF 5.726
Liwei Zhang,Huaike Zhang,Zhiqiang Chen,Qiang Ning,Suyao Liu,Jie Ren,Xiaodong Wen,Yong-Wang Li
To correlate the relationship between the Al distribution and the catalytic performance of long-chain olefin aromatization, several ZSM-5 zeolites with different Al locations and proximities were prepared via adjusting the hydrothermal synthesis conditions. Characterization by XRD, SEM and N2-adsorption indicated that all the as-prepared ZSM-5 zeolites exhibited high crystallinities, uniform morphologies and open channels. However, a great difference in the framework Al (AlF) positions was observed by 27Al MAS NMR and UV-vis-DRS of Co(II) ions, thus leading to the distinct acidity of various zeolites as determined by NH3-TPD and Py-IR. In 1-octene aromatization reaction, ZSM-5 catalysts exhibited significantly different catalytic performances and reaction pathways, which were closely related to the types and locations of the AlF atoms. The ZSM-5 zeolite catalyst with the most abundant single Al sites exhibited the lowest aromatics selectivity, while Al pairs in the framework were more favorable to the formation of aromatics. Moreover, the product distribution revealed that the Al pairs in the channel intersections of the ZSM-5 zeolite were responsible for the improved selectivity to heavy aromatics due to the fewer space restrictions; however, the Al pairs in the sinusoidal and straight channels with severe size restrictions suppressed the generation of bigger aromatics on the Al sites. This result provided a new potential method for precisely tailoring the catalytic selectivity in olefin conversion and upgrading the Fischer–Tropsch oil.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Haiyun Li,Yizhi Qiu,Tuo Yao,Yachun Ma,Huirong Zhang,Xiaolei Yang,Changning Li
Environmental problems caused by the large-scale use of chemical pesticides are becoming more and more serious, and the removal of chemical pesticides from the ecological environment by microbial degradation has attracted wide attention. In this study, using enrichment screening with seven chemical pesticides as the sole carbon source, a mixed microbial culture (PCS-1) was obtained from the continuous cropping of strawberry fields. The microbial community composition, degradation ability, and detoxification effect of PCS-1 was determined for the seven pesticides. Inoculation with PCS-1 showed significant degradation of and tolerance to the seven pesticides. Microbial community composition analysis indicated that Pseudomonas , Enterobacter , Aspergillus , and Rhodotorula were the dominant genera for the degradation of the seven pesticides by PCS-1. The concentration of the seven pesticides was 10 mg L −1 in hydroponic and soil culture experiments. The fresh weight, plant height, and root length of PCS-1-inoculated alfalfa ( Medicago sativa ) significantly increased compared with those of non-PCS-1-inoculated M. sativa . PCS-1 not only effectively degraded the residual content of the seven pesticides in water and soil but also reduced the pesticide residues in the roots, stems, and leaves of M. sativa . This study shows that PCS-1 may be important in environmental remediation involving the seven pesticides.
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MICROVASCULAR RESEARCH
·
2021
·
IF 3.514
Yi-hua Zhou,Yu-zhi Tang,Liang-yun Guo,Li-li Zheng,Dan Zhang,Can-ying Yang,Wei Wang
Soluble fms-like tyrosine kinase-1 (sFlt-1), a circulating antiangiogenic protein, is involved in the pathogenesis of atherosclerosis (AS), and the underlying mechanism is still unclear. Here, we attempted to investigate the mechanism of action of sFlt-1 in AS. Human umbilical vein endothelial cells (HUVECs) were treated with oxidized low density lipoprotein (ox-LDL) to induce cell injury. ox-LDL treatment increased LC3-II/LC3-I ratio, Beclin-1 expression and GFP-LC3 puncta in HUVECs, suggesting that ox-LDL may induce autophagic flux impairment in HUVECs. ox-LDL-treated HUVECs displayed a decrease of sFlt-1 levels. Moreover, ox-LDL treatment reduced cell proliferation and elevated apoptosis in HUVECs, which was abrogated by sFlt-1 overexpression. Up-regulation of sFlt-1 repressed the activity of PI3K/AKT/mTOR signaling pathway and enhanced autophagy in HUVECs following ox-LDL treatment. Additionally, sFlt-1 overexpression-mediated increase of autophagy in ox-LDL-treated HUVECs was abolished by 3-methyladenine (autophagy inhibitor). 3-methyladenine abrogated the impact of sFlt-1 overexpression on proliferation and apoptosis in ox-LDL-treated HUVECs. This work confirmed that overexpression of sFlt-1 activated autophagy by repressing PI3K/Akt/mTOR signaling pathway, and thus alleviated ox-LDL-induced injury of HUVECs. Therefore, this study suggests that sFlt-1 may be a potential target for AS treatment.
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Redox Biology
·
2026
·
IF 16.2
Zhengyi Chen,Xidan Fu,Chao Liu,Wenkang Jiang,Jiurong Liu,Miao Hao,Ling Fu,Wei Huang,Xiujuan Lang,Xijun Liu,Yumei Liu,Hulun Li,Rui Li,Bo Sun
Aging reshapes hematopoiesis toward myelopoiesis, particularly increased output of classical monocytes, contributing to immunosenescence. However, the underlying mechanisms remain unclear. Here, we identify that aging drives hematopoietic stem cells (HSCs) to progressively commit to a CCR2 + monocyte fate along a defined lineage trajectory, which is executed through a redox-sensitive transcriptional cascade. We find that the age-related decline in bone marrow IGF-1 is the upstream niche signal that releases suppression of the oxidative stress/NF-κB/IRF8 axis, allowing this cascade to redirect early progenitors toward a CCR2 + myeloid-primed fate. Functional assays confirm that cells following this CCR2 + trajectory drive myeloid-biased hematopoiesis and directly contribute to age-associated immunosuppression. Our work delineates a hierarchical pathway from niche decay to lineage redirection, establishing the CCR2 + fate trajectory as a key driver of immunosenescence and a potential therapeutic target.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2023
·
IF 3.8
Chenhao Wei,Di Gao,Guohao Zhang,Liang Zhao,Jinsen Gao,Chunming Xu
1-Hexene aromatization is a promising technology to convert excess olefin in FCC gasoline to high-value benzene (B), toluene (T), and xylene (X). Besides, the increasing market demand of xylene has put forward higher requirements for new generation of catalyst. For increasing xylene yield in 1-hexene aromatization, the effect of mesopore structure and spatial distribution on product distribution and Zn loading was studied. Catalysts with different mesopore spatial distribution were prepared by post-treatment of parent HZSM-5, including NaOH treatment, Tetra-propylammonium hydroxide (TPAOH) treatment, and recrystallization. It was found the evenly distributed mesopore mainly prolongs the catalyst lifetime by enhancing diffusion properties but reduces the aromatics selectivity, as a result of damage of micropores close to the catalyst surface. While the selectivity of high-value xylene can be highly promoted when the mesopore is mainly distributed interior the catalyst. Besides, the state of loaded Zn was also affected by mesopores spatial distribution. On the optimized catalyst, the xylene selectivity was enhanced by 12.4% compared with conventional Zn-loaded parent HZSM-5 catalyst at conversion over 99%. It was attributed to the synergy effect of mesopores spatial distribution and optimized acid properties. This work reveals the role of mesopores in different spatial positions of 1-hexene aromatization catalysts in the reaction process and the influence on metal distribution, as well as their synergistic effect two on the improvement of xylene selectivity, which can improve our understanding of catalyst pore structure and be helpful for the rational design of high-efficient catalyst.
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JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Yu Miao,Fangzheng Yan,Ye Yuan,Fengrui Yang,Bin Sun,Zhi Wang
Loose nanofiltration membranes (LNMs) prepared by co-deposition of polyphenols and amine-rich polymers has been considered as an effective method for the efficient treatment of textile wastewater. However, the dye/salt selectivity remains unsatisfactory, due to the low free volume with wide distribution caused by the stacking and tight entanglement of polymeric chain segments. A high dye/salt selective LNM was prepared for the first time by rapid co-deposition of high-valent metal-induced microporous polymer framework (HMMP-1) and dopamine (DA). HMMP-1 with high specific area and suitable pore size can provide additional transfer channels for water/salt, breaking the limitation of free volume generated by polymer stacking and further improving perm-selectivity. In addition, the amine-rich HMMP-1 with a flexible polymer framework can be covalently linked to DA by Michael addition/Schiff base reaction, effectively avoiding the selectivity reduction caused by poor interfacial compatibility. The results showed that the pure water permeance of the optimal membrane (LNM-2) was 57.22 LMH·bar −1 , which was 0.65 times higher than that of LNM-1 (without HMMP-1). In addition, LNM-2 exhibited excellent dye/salt selectivity for dye/salt mixtures with different concentrations, reaching >47 (CR/NaCl) and >29 (CR/Na 2 SO 4 ), respectively, which is more than twice of the conventional membranes reported so far. Moreover, the dye/salt selectivity of LNM-2 still maintained at 28 (CR/Na 2 SO 4) and 55 (CR/NaCl) respectively for 12 h, when salt concentration was 10 g L −1 , which fully demonstrated the good stability of LNM-2 and the superiority of HMMP-1 for the design of LNM.
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ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Xiankang Fan,Nan Ling,Chunli Liu,Mingzhen Liu,Jue Xu,Tao Zhang,Xiaoqun Zeng,Zhen Wu,Daodong Pan
In this study, an efficient cholesterol-lowering strain of Lactiplantibacillus plantarum 54–1 was screened and its degradation molecular mechanism was investigated. Furthermore, a novel practical MRS medium for screening cholesterol-lowering lactic acid bacteria (LAB) was developed based on ultrasound treatment. L. plantarum 54–1 was found to have the highest ability to eliminate cholesterol (340.69 ± 5.87 µg/mL). According to SEM and the count of viable LAB results, the morphology of LAB in the cholesterol-containing medium developed in this experiment was close to the normal (full and smooth), and it can grow normally. Metabolomics revealed that L. plantarum 54–1 initially converted a portion of cholesterol to 7α-hydroxy-cholesterol and then to the key metabolite taurine, via the phosphotransferase system. These metabolites were further transformed into L-alanine, L-lysine, N6-Acetyl-L-lysine, (R)-b-aminoisobutyric acid, and 2-oxoarginine, through glycine, serine, and threonine metabolism, citrate cycle, D-arginine and D-ornithine metabolism, lysine degradation, and pyruvate metabolism pathways. Prokaryotic reference transcriptomics found that this may be mainly regulated by the bsh , phnE , ptsP , B0667_RS04545, and B0667_RSRS12300 genes, which was further validated by qPCR. Furthermore, molecular docking results demonstrated that 8 differential metabolites might bind to another portion of cholesterol via PI-PI conjugation and hydrophobic interactions and lower cholesterol via co-sedimentation. This study has strategic implications for developing probiotic powder food that lowers cholesterol.
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WATER RESEARCH
·
2023
·
IF 12.8
Di Cao,Xueqi Chen,Jun Nan,Aijie Wang,Zhiling Li
Influences of heavy metal exposure to the organohalide respiration process and the related molecular mechanism remain poorly understood. In this study, a non-obligate organohalide respiring bacterium, Pseudomonas sp. strain CP-1, was isolated and its molecular response to the five types of commonly existed heavy metal ions were thoroughly investigated. All types of heavy metal ions posed inhibitory effects on 2,4,6-trichlorophenol dechlorination activity and cell growth with the varied degree. Exposure to Cu (II) showed the most serious inhibitive effects on dechlorination even at the lowest concentration of 0.05 mg/L, while the inhibition by As (V) was the least with the removal kinetic constant k decreased to 0.05 under 50 mg/L. Further, multi-omics analysis found compared with Cu (II), As (V) exposure led to the insignificant downregulation of a variety of biosynthesis processes, which would be one possible account for the less inhibited activity. More importantly, the inhibited mechanisms on the organohalide respiration catabolism of strain CP-1 were firstly revealed. Cu (II) stress severely downregulated NADH generation during TCA cycle and electron donation of organohalide respiration process, which might decrease the reducing power required for organohalide respiration. While both Cu (II) and As (Ⅴ) inhibited substrate level phosphorylation during TCA cycle, as well as electron transfer and ATP generation during organohalide respiration. Meanwhile, CprA-2 was confirmed as the responsible reductive dehalogenase in charge of 2,4,6-TCP dechlorination, and transcriptional and proteomic studies confirmed the directly inhibited gene transcription and expression of CprA-2. The in-depth reveal of inhibitory effects and mechanism gave theoretical supports for alleviating heavy metal inhibition on organohalide respiration activity in groundwater co-contaminated with organohalides and heavy metals.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 11
·1-9
·
IF 5.7
Wang Jing,Shi Lu,Tang Qiaorong,Zhu Xinyu,Wu Mengmeng,Liu Wei,Li Baoxin,Jin Yan
Nucleic acid lateral flow assay (NALFA) with gold nanoparticles (AuNPs) as colorimetric probes have been extensively adopted for point-of-care testing (POCT). However, the sensitivity of NALFA still needs to be improved. Herein, DNA-gold nanoaggregate (DNA-AuNA) was assembled as a signal amplification probe of NALFA for sensitive detection of tumor marker TK1 mRNA. Four functional oligonucleotides with complementary pairs were assembled to form DNA-AuNA that coupled more AuNPs to improve sensitivity. Thus, the limit of detection (LOD) was 0.36 pM, which is lower than that of conventional AuNPs-based NALFA. Moreover, the bioassay showed good reproducibility, stability, and specificity for detecting TK1 mRNA. The detection of TK1 mRNA in human serum was also satisfactory. Therefore, DNA-AuNA-based NALFA provides a sensitive method for portable detection of TK1 mRNA. Graphical abstract
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FUEL PROCESSING TECHNOLOGY
·
2023
·
IF 7.5
Ke Li,Mengxia Yan,Haili Wang,Lifen Cai,Ping Wang,Huimin Chen
As a typical high-efficient alkane aromatization catalyst, Pt/KL is prone to deactivation due to the sintering growth of Pt on the outer surface of KL and the channel blockage caused by carbon deposition , which is further exacerbated by the one-dimensional channel structure of KL. Therefore, a series of encapsulated catalysts combining the three-dimensional channel structure advantages of S-1 with the role of potassium are prepared and evaluated. It is shown that the encapsulated catalyst KPt@S-1-02 modified by moderate potassium exhibits extremely superior stability and no significant deactivation is observable after n-heptane aromatization for 180 h under harsh conditions. On the one hand, encapsulation structure enables a strong anti-agglomeration ability of Pt and the topological structure of S-1 endows the encapsulation catalysts less susceptible to channel blockage, which has been demonstrated by HAADF-STEM and N 2 -adsorption. On the other hand, characterizations involving NH 3 -TPD, CO-DRIFT and HAADF-STEM verify that the modification by an appropriate amount of K can inhibit acid sites of catalysts and improve Pt dispersion and electron density, thus enhance the stability of catalysts. However, excessive K can serve as weak acid sites, poison of active sites and inhibitor of H 2 adsorption on Pt surface, leading to deactivation of the catalyst.
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CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Yulin Yao,Zonghan Yang,Pengfei Zheng,Zhenheng Diao
The accessible TiO 6 concentration and the hydrophobicity of hierarchical TS-1 zeolites are both considered as critical factors for promoting the catalytic performance of selective oxidation. However, simultaneously enhancing these two factors still remains a challenge. Herein, hierarchical TS-1 zeolites with high hydrophobicity and concentration of accessible TiO 6 were fabricated via sequential amphiphilic organosilane-assisted pre-treatment, dry-gel conversion and hydroxyl radical post-treatment, using amphiphilic organosilane and surfactant as meso -porogens. The synthesis mechanism was investigated, and it was found that the interaction between the amphiphilic organosilane and Ti species formed during the pre-treatment benefited the formation of accessible TiO 6 during the subsequent dry-gel conversion. During the post-treatment of zeolite with H 2 O 2 solution under ultraviolet light irradiation, appropriate H 2 O 2 concentration facilitated the generation of hydroxyl radical and thus enhanced the hydrophobicity of zeolite without significantly influencing the TiO 6 concentration. The hierarchical TS-1 displayed excellent catalytic performances in 1-hexene epoxidation and oxidative desulfurization, with conversion improvements of 91.2%±2.8% and 348%±17%, respectively, compared with the conventional counterpart.
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危险品化学品经营许可证（带存储）