JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2019
·
IF 3.31
Ning Wang,Xinxin Han,Junya Li,Ying Wang,Wenquan Yu,Ruiyong Wang,Junbiao Chang
In this study, the interaction between 3-phenyl-1H-indazole (1a) and the fat mass and obesity-associated (FTO) protein was confirmed by isothermal titration calorimetry (ITC). The structure feature of 1a was different from our previously reported FTO inhibitors (radicicol, N-CDPCB and CHTB); the Cl and diol group in structure motif is critical for inhibitors to bind to FTO. In order to test whether there is specificity for the interaction between FTO and 1a, the interactions between 1a and four important proteins (human serum albumin (HSA), pepsin, catalase and trypsin) were investigated by ITC, spectroscopy and molecular docking methods. ITC results showed spontaneous exothermic reactions occurring between 1a and the proteins except trypsin under investigated conditions. The order of the binding affinity of 3-phenyl-1H-indazole is catalase > HSA > FTO > pepsin. Comparison between ITC and spectral results was made. This work will provide the basis for the design of novel inhibitors for FTO.
AbbreviationsCAT=catalaseDMSO=dimethyl sulfoxideFTO=fat mass and obesity-associated proteinHSA=human serum albuminPep=pepsinTry=trypsinCommunicated by Ramaswamy H. Sarma
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Wei Xu,Zhen Qin,Yutong Hao,Qiang He,Shuai Chen,Zhongshuang Zhang,Dan Peng,Haiyan Wen,Jun Chen,Jingfu Qiu,Chaorui Li
In this work, hollow nanobox metal-organic framework (HNM) nanocomposites were synthesised and utilised for the first time in a signal decreased electrochemical immunosensor for the ultrasensitive quantitative determination of lymphocyte activation gene-3 (LAG-3) protein, which is a newly discovered biomarker. With the aid of signal materials, namely, SiO 2 -tagged anti-LAG-3 antibody (SiO 2 -Ab 2 ) and the biotin-streptavidin system, the sensor can achieve signal amplification . Encapsulation of tin dioxide-functionalised reduced graphene oxide (rGO-SnO 2 ) and gold and platinum alloys (AuPt alloys) onto the surface of hollow nanobox metal-organic frameworks (MOFs) was performed to prepare rGO-SnO 2 /hollow nanobox-MOFs/AuPt alloys (rGO-SnO 2 /HNMs/AuPt) as the matrix. SiO 2 -Ab 2 , which is used as the signal-decreased label, can be utilised to enhance the distinction of the electrochemical signal after the specific recognition between antibodies and antigens, owing to its large steric hindrance property. In this sensor, this proposed sandwich immunosensor can achieve a high sensitivity, especially in the presence of low concentrations of the LAG-3 protein. Under optimal conditions, this sandwich-designed immunosensor exhibited a sensitive detection of the LAG-3 protein from concentrations of 0.01 ng mL −1 to 1 μg mL −1 , with a lower detection limit of 1.1 pg mL −1 (based on 3σ). We proposed that this ultrasensitive biosensor can be utilised for the detection of the LAG-3 protein in early clinical tumour diagnosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN TOXICOLOGY
·
2018
·
IF 3.432
Xiaomin Xia,Bin Lu,Wenjing Dong,Bingwei Yang,Yawen Wang,Qi Qin,Zixuan Liu,Erqun Song,Yang Song
Our previous study showed that tetrachlorobenzoquinone (TCBQ) mediated the activation of Nod-like receptor protein 3 (NLRP3) inflammasome, which involves K+ efflux, reactive oxygen species (ROS) production, and mitochondrial DNA damage. In addition, TCBQ down-regulates NLRP3 ubiquitination and promotes the activation of NLRP3 inflammasome. However, the induction of NLRP3 inflammasome by atypical pathways has not yet been characterized. Using human umbilical vein endothelial cells (HUVEC), we discovered that TCBQ activates caspase 1/4/5 and cleaves gasdermin D (GSDMD) into N-terminal and C-terminal cleavage products. In parallel, TCBQ also activates receptor interacting protein kinase 3 (RIPK3)/mixed lineage kinase domain-like protein (MLKL) signaling pathways. The N-terminal fragments of GSDMD and MLKL translocate from cytoplasm to cell membrane and form oligomers and membrane pores on the cell membrane. The formation of membrane pores not only promotes the extracellular secretion of interleukin 1 beta (IL-1β) but also affects cellular ion homeostasis, in particular promotes K+ outflow, which further activates NLRP3 inflammasome and aggravates cellular inflammation. These results indicated that GSDMD and MLKL play important roles in TCBQ-induced endothelial pro-inflammatory responses, which may point to potential therapeutic approaches for TCBQ-mediated toxicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CATALYSIS
·
2018
·
IF 6.759
Yuqing Wang,Li Yan,Cunyao Li,Miao Jiang,Ziang Zhao,Guangjin Hou,Yunjie Ding
Porous organic copolymer (designated as CPOL-BP&P(OPh) 3 ) containing vinyl biphephos moieties and tris(4-vinylphenyl)phosphite (P(OPh) 3 ) moieties was synthesized through a solvothermal copolymerization technique. After metalation with Rh precursor, an efficient heterogeneous catalyst Rh/CPOL-BP&P(OPh) 3 for the hydroformylation of 1-butene is presented with high activity (TOF = 2490.3 h −1 ) and regioselectivity (l:b = 40.0). The Rh/CPOL-BP&P(OPh) 3 catalyst has been investigated by using solid-state 13 C MAS NMR, in situ FT-IR spectroscopy, nitrogen sorption measurements, SEM, TEM, and XPS. Interestingly, in situ FT-IR spectra showed that Rh species tended to coordinate with both two kinds of P species from biphephos and P(OPh) 3 ligands in the Rh/CPOL-BP&P(OPh) 3 polymer frame rather than one kind of them. Therefore, the synergistic effect of three kinds of coordination compound formed between Rh species and both ligands could be responsible for the high activity and regioselectivity observed in hydroformylation of 1-butene.
✓ 已验证
产品关联
DOI
高影响因子
Chinese Journal of Natural Medicines
·
2018
·
IF 1.991
Yan-Zi LI,Zhi-Na MA,Yin-Shi SUN,Shen REN,Shuang JIANG,Wei-Zhe ZHANG,Zi WANG,Wei LI
The present study was designed to evaluate protective activity of an ethanol extract of the stems of Schisandra chinensis (SCE) and explore its possible molecular mechanisms on acetaminophen (APAP) induced hepatotoxicity in a mouse model. The results of HPLC analysis showed that the main components of SCE included schisandrol A, schisandrol B, deoxyschisandrin, schisandrin B, and schisandrin C and their contents were 5.83, 7.11, 2.13, 4.86, 0.42 mg·g −1 , respectively. SCE extract was given for 7 consecutive days before a single hepatotoxic dose of APAP (250 mg·kg −1 ) was injected to mice. Our results showed that SCE pretreatment ameliorated liver dysfunction and oxidative stress, which was evidenced by significant decreases in aspartate transaminase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) contents and elevations in reduced glutathione (GSH) and superoxide dismutase (SOD) levels. These findings were associated with the result that the SCE pretreatment significantly decreased expression levels of 4-hydroxynonenal (4-HNE) and 3-nitrotyrosine (3-NT). SCE also significantly decreased the expression levels of Bax, mitogen- activated protein kinase (MAPK), and cleaved caspase-3 by APAP exposure. Furthermore, supplementation with SCE suppressed the expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), suggesting alleviation of inflammatory response. In summary, these findings from the present study clearly demonstrated that SCE exerted significant alleviation in APAP-induced oxidative stress, inflammation and apoptosis mainly via regulating MAPK and caspase-3 signaling pathways.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Weijun Chen,Wenjun Wang,Xiaobin Ma,Ruiling Lv,Ritesh Balaso Watharkar,Tian Ding,Xingqian Ye,Donghong Liu
Effect of pH-shifting on structural and functional properties of whey protein isolate and its interaction with (−)-epigallocatechin-3-gallate were investigated. Circular dichroism spectra showed that pH-shifting induced the decrease in α-helix content by 12.18% and β-sheet content by 3.24%, but β-turn and random coil content increased by 4.26% and 5.91%, respectively. Increase of fluorescence intensity and red-shift of maximum emission wavelength indicated the structural unfolding and exposure of tyrosine. The treatment also significantly increased the surface hydrophobicity, disulfide bonds content, solubility, emulsifying activity and emulsion stability of whey protein isolate at P < 0.05 level. Fluorescence quenching analysis revealed that treated whey protein isolate have a stronger binding affinity to (−)-epigallocatechin-3-gallate, resulting a better protection against the degradation of (−)-epigallocatechin-3-gallate and its antioxidant activity. This study confirmed that pH-shifting treatment can improve functional properties of whey protein isolate and its potential as a protective carrier for polyphones.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHIRALITY
·
2018
·Vol. 30
·期 6
·769-776
·
IF 1.833
Yilong Wang,Yongkai Xu,Yun Zhang,Aijun Sun,Yunfeng Hu
The two enantiomers of ethyl 3-hydroxybutyrate are important intermediates for the synthesis of a great variety of valuable chiral drugs. The preparation of chiral drug intermediates through kinetic resolution reactions catalyzed by esterases/lipases has been demonstrated to be an efficient and environmentally friendly method. We previously functionally characterized microbial esterase PHE21 and used PHE21 as a biocatalyst to generate optically pure ethyl ( S )-3-hydroxybutyrate. Herein, we also functionally characterized one novel salt-tolerant microbial esterase WDEst17 from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. Esterase WDEst17 was further developed as an efficient biocatalyst to generate ( R )-3-hydroxybutyrate, an important chiral drug intermediate, with the enantiomeric excess being 99% and the conversion rate being 65.05%, respectively, after process optimization. Notably, the enantio-selectivity of esterase WDEst17 was opposite than that of esterase PHE21. The identification of esterases WDEst17 and PHE21 through genome mining of microorganisms provides useful biocatalysts for the preparation of valuable chiral drug intermediates.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2012
·Vol. 1
·期 7
·2508-2514
·
IF 0
Jing Su,Xing-Long Wu,Jong-Sook Lee,Jaekook Kim,Yu-Guo Guo
A facile sol–gel approach combined with a carbon-coating technique via high-temperature thermally decomposing C2H2 has been developed for the synthesis of a Li3V2(PO4)3/C (LVP/C) cathode material employing the biomass of phytic acid as an eco-friendly phosphorus source. The effects of the carbon-coating on the structural, morphological and electrochemical properties of LVP have been investigated. Compared with pristine LVP, the LVP/C composite presents a higher discharge capacity of 127 mA h g−1 at 0.1 C, better rate capability and long-term cyclability in the voltage range of 3.0–4.3 V. Even at a high charge–discharge rate of 5 C, it can still deliver a reversible capacity of 107 mA h g−1 over 400 cycles without obvious fading, demonstrating great potential as a superior cathode material for lithium-ion batteries.
✓ 已验证
产品关联
DOI
CHEMICAL ENGINEERING JOURNAL
·
2014
·
IF 4.058
Jiansheng Huo,Yuhui Zheng,Qianming Wang
Novel red-emitting Eu 3+ -activated La 2 − x Gd x (MoO 4 ) 3 − y (WO 4 ) y phosphors with well-defined one-dimensional nanostructures were synthesized via a supersonic microwave co-assistance (SMC) method for the first time at very low temperature (60 °C). Scanning electron microscopy studies substantiated that these phosphors are very uniform. The crystal structure and luminescent properties were characterized by powder X-ray diffraction patterns (XRD) and photoluminescence measurements (PL) respectively. An intense red emission at 615 nm ( 5 D 0 → 7 F 2 ) was detected under the excitation at 280 nm. The PL properties of La 2 − x Gd x (MoO 4 ) 3 − y (WO 4 ) y : Eu 3+ with different ratio of La/Gd or MoO 4 /WO 4 have also been investigated in detail. Furthermore, the optimized phosphor was embedded into the poly-hydroxyethylmethacrylate(HEMA) gel and the corresponding luminescent gel was achieved.
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
·
2013
·
IF 3.552
Siyuan Sun,Kai Wang,Hongmei Lei,Liping Li,Meijuan Tu,Su Zeng,Hui Zhou,Huidi Jiang
Organic cation transporter 2 (OCT2) and 3 (OCT3) are low-affinity, high-capacity transporters (uptake-2) expressed in the central nervous system (CNS) and other major organs. Proven to be essential components in the CNS functions , OCT2 and OCT3 are suggested as potential targets of antidepressant therapeutics recently. Berberine , an active constituent derived from many medicinal plants , such as Coptis chinensis , has been reported to possess antidepressant-like action in the tail suspension test and forced swim test with elevated serotonin/norepinephrine/dopamine (5-HT/NE/DA) level in mouse brain ; however the mechanism has not been elucidated. In consideration of the relation between OCT2/3 and antidepressant action, and the characteristic of berberine as an organic cation, we investigated the potential involvement of OCT2 and OCT3 in the antidepressant-like action of berberine in the present study. The results in mouse brain synaptosomes demonstrated that uptake-2 inhibition might play a notable role in enhanced serotonergic and noradrenergic effects induced by berberine. The inhibitory study in transfected MDCK cells displayed that berberine is a potent inhibitor of human OCT2 and OCT3, and its IC 50 values for inhibition of transporter-mediated 5-HT/NE uptake are between 0.1 and 1 μM. In addition, berberine was identified as a substrate of hOCT2 and hOCT3. In conclusion, berberine is a substrate and an inhibitor of hOCT2 and hOCT3, and its inhibition on OCT2- and OCT3-mediated 5-HT and NE uptake may contribute to the enhanced monoamine neurotransmission in mouse brain. It was deduced that the inhibition of OCT2 and OCT3 probably be implicated in the mechanism of antidepressant-like action.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2014
·
IF 2.726
Yue Yang,Shengming Xu,Ming Xie,Yinghe He,Guoyong Huang,Youcai Yang
Spherical Ni 1/3 Co 1/3 Mn 1/3 (OH) 2 agglomerates were synthesized by the co-precipitation method in the presence of ammonia. The results show that the growth mechanism of spherical agglomerates follows three-stages, i.e. nucleation and anisotropic growth of single crystals; agglomeration of polycrystalline crystallites agglomerated by single crystal grains as primary particles to form embryonic agglomerates; formation, growth and consolidation of spherical agglomerates or particles by agglomeration of embryonic agglomerates, continued growth of individual crystals in the agglomerates and further attachment of primary particles. The first two stages are very fast while the last stage takes almost the entire process to complete. The main reason for the anisotropic growth of Ni 1/3 Co 1/3 Mn 1/3 (OH) 2 crystal is that crystal surface energy of E (001) , E (100) , E (101) and E (102) is different with E (001) being the highest. The morphology of the final spherical agglomerates is explained by partial re-crystallization of contacting primary particles. The growth process of spherical agglomerates was examined by X-ray diffraction, scanning electron microscope, transmission electron microscope and calculation of crystal surface energy using density function theory.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 28
·21504-21510
·
IF 3.84
Xiaojuan Zhao,Fengxia Li,Qingyan Zhang,Zengbo Li,Yehong Zhou,Jun Yang,Chuan Dong,Junping Wang,Shaomin Shuang
Mn-doped ZnS quantum dots capped with 3-mercaptopropionic acid (MPA) were synthesized by a facile method in aqueous solution as a ratiometric fluorescent (I590 nm/I458 nm) probe for curcumin. Curcumin caused defect-related fluorescence quenching of the probe at 458 nm, whereas the orange transition emission of the probe at 590 nm changed only negligibly with variations in curcumin concentration. The MPA-assembled Mn-doped ZnS quantum dots were used for the simple, sensitive and non-toxic determination of curcumin. Under the optimized conditions, the response to curcumin was linear in the range 0.16–16.9 μM with a detection limit of 37 nM. The method was successfully applied to the determination of trace amounts of curcumin in urine samples with recoveries of 97.73–109.73%.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）