Journal of Biomedical Nanotechnology
·
2013
·Vol. 9
·期 5
·863-869
·
IF 5.256
Zhang Li,Liu Yongjun,Yu Dexin,Zhang Na
The aim of our study was to prepare gadolinium loaded chitosan nanoparticles (Gd-CSNPs) for magnetic resonance imaging (MRI). The chitosan nanoparticles (CSNPs) were prepared by ionic gelation method with sodium tripolyphosphate. The Gd ions were conjugated to the surface of CSNPs through
diethylenetriamine pentaacetic acid (DTPA) using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to obtain Gd-CSNPs. The physicochemical properties of CSNPs and Gd-CSNPs were measured by transmission electron microscope,
dynamic light scattering and inductively coupled plasma optical emission spectroscopy, respectively. The cell toxicity evaluation was performed in mouse B16 cells by MTT assay. The T1-weighed magnetic resonance images were measured by a 3.0 T Sigma scanner. The morphologies of the CSNPs and
Gd-CSNPs were spherical or ellipsoidal in shape. The mean sizes of the CSNPs and Gd-CSNPs were 110.9 ± 6.8 nm and 153.0 ± 7.5 nm, respectively. The zeta potentials of the CSNPs and Gd-CSNPs were 22.30 ± 0.77 mV and 13.91 ± 4.26 mV, respectively. The relaxation rates
of Gd-CSNPs and Magnevist
®
were 7.509 mM–1 · s–1 and 3.052 mM–1 · s–1, respectively. The Gd-CSNPs exhibited high T1 relaxivity and no obvious cytotoxicity was observed under the experimental
concentrations in mouse B16 melanoma cells. These results indicated that the Gd-CSNPs had great potential as MRI contrast agents for the early diagnosis of tumor.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Xiuge Gao,Jiahao Gong,Ying Cai,Jiacai Wang,Jia Wen,Lin Peng,Hui Ji,Shanxiang Jiang,Dawei Guo
As immune adjuvants assisting vaccines, nanoparticle delivery systems have been widely exploited. Squalene, the major ingredient of approved adjuvant MF59, has great potential in activating immune responses. In the current study, model antigen ovalbumin (OVA) was encapsulated into squalene-based nanostructured lipid carriers (NLCs), and the chitosan, a cationic polysaccharide, was used for modifying nanoparticles to develop a functionalized and cationic nanoparticle delivery system (OVA-csNLCs). Firstly, the optimal formulation of csNLCs was successfully screened out, and had hydrodynamic diameter of 235.80 ± 5.99 nm and zeta potential of 34.90 ± 6.95 mV. Then, the generated OVA-csNLCs had no significant difference in hydrodynamic diameter and exhibited lower zeta potential of 19.03 ± 0.31 mV and high encapsulation efficiency of 83.4%. Sucrose (10%, w /w) was selected as optimal lyoprotectant, exhibiting good stability of OVA-csNLCs in the form of freeze-dried powder. More importantly, the OVA-csNLCs effectively promoted OVA antigen uptake by macrophage, significantly enhanced the level of OVA-specific IgG, and induced a Th2-based immune response in vivo. Furthermore, mice immunization experiment demonstrated that OVA-csNLCs had well biocompatibility and facilitated spleen lymphocytes proliferation. Above findings indicate that chitosan modified squalene nanostructured lipid carriers show promise as antigen delivery system and an open adjuvant platform.
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Polymers
·
2025
·Vol. 17
·期 23
·3099
·
IF 4.9
Yujia Wang,Yanling Bao,Yongtao Yao,Sijia Chen,Wenpeng Tang,Jiawen Liu,Juncheng Wang,Zhigang Li,Bo Tian,Shibo Wu,Hongtao Zhao,Pengfei Huo,Jidong Dong,Dawei Zhang
Zinc ions, as essential trace elements in the human body, play a crucial role in promoting wound healing. They have significant applications in the medical field. In this paper, chitosan (CS)/polyvinyl alcohol (PVA)/zinc gluconate hydrogel was synthesized via gamma ray irradiation cross-linking. The hydrogel exhibited excellent antibacterial properties, and could continuously release zinc ions. Antibacterial assays demonstrated that the combination of CS and zinc ions improved the antibacterial properties of hydrogel. The inhibition zones against bothStaphylococcus aureusandEscherichia coliexceed 12 mm. The cell viability can reach 108.25%. The prepared hydrogel could continuously release zinc ions over a period of 70 h. The pore and chemical structure were, respectively, performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). And its comprehensive properties, such as gel content, water evaporation ratio and swelling behavior were investigated. The hydrogels showed potential application value in the hydrogel dressing for zinc ion delivery.
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Foods
·
2025
·Vol. 15
·期 1
·25
·
IF 5.1
Jing Li,Can Luo,Changchun Li,Bin Li
Soy protein isolate (SPI)-based foods were limited by beany flavors and suboptimal emulsifying performance. Conventional flavor-mitigation strategies were often accompanied by losses in protein functionality, and the potential of ultrasound to simultaneously modulate structure, functionality, and flavor release of SPI–chitosan (SPI–CS) coacervates remained unclear. In this study, SPI–CS complexes were prepared and subjected to ultrasound at amplitudes of 20%, 50%, and 80% for 2 min, 5 min, or 10 min, after which their physicochemical properties, emulsifying properties, and the release of beany-flavor compounds were characterized. Coacervation with CS was found to increase particle size, decrease solubility, and enhance emulsifying indices while reducing beany-flavor release relative to SPI alone. Ultrasound treatment further decreased particle size, increased the absolute ζ-potential and surface hydrophobicity, and induced changes in secondary structure that were associated with improved solubility and emulsifying properties. Moderate amplitudes (20%, 50%) were more effective in enhancing emulsifying activity and alleviating flavor release, whereas prolonged treatment at 80% amplitude resulted in partial reaggregation and compound-dependent flavor behavior. Overall, ultrasonic processing was demonstrated to be a tunable, green strategy for engineering SPI–CS coacervates with concurrently improved functional and flavor attributes.
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Shao-qing Chen,Yan-qing Song,Cheng Wang,Shan Tao,Fang-ying Yu,Hai-ya Lou,Fu-qiang Hu,Hong Yuan
Targeted and sensitive drug release at the colitis site is critical for the effective therapy of ulcerative colitis and reduction of side effects from the drug. Herein, we used 3,3′-dithiodipropionic acid (DTPA) to covalently link quercetin (Qu) and glyceryl caprylate-caprate (Gcc) via ester bonds to prepare Qu-SS-Gcc lipid nanoparticles (Qu-SS-Gcc LNPs). Dexamethasone (Dex) was used as a model drug, and chitosan (CSO) was modified on the surface of Qu-SS-Gcc LNPs to obtain CSO-modified Dex-loaded Qu-SS-Gcc LNPs (CSO/Dex/LNPs). The encapsulation efficiency and drug loading of CSO/Dex/LNPs were 93.1 % and 8.1 %, respectively. The in vitro release results showed that CSO/Dex/LNPs had esterase-responsive characteristics and could release the drug rapidly in esterase-containing artificial intestinal fluid. A human colorectal adenocarcinoma cell (Caco-2) monolayer was used as the intestinal cell barrier model. Transmembrane resistance measurements and permeation experiments showed that CSO/Dex/LNPs had a protective effect on the lipopolysaccharide (LPS)-stimulated Caco-2 cell monolayer and increased the expression of E-cadherin in LPS-stimulated Caco-2 cells. Moreover, CSO/Dex/LNPs could significantly reduce the expression of the inflammatory factors TNF-α, IL-6 and NO in LPS-stimulated RAW 264.7 cells. The ulcerative colitis mouse model was constructed by using C57BL/6 mice. The in vivo distribution results showed that CSO/Dex/LNPs had colon-targeting effects and strong retention ability in the colons of mice with colitis. The results also showed that CSO/Dex/LNPs had better anti-inflammatory effects than free Dex, which could reduce colonic atrophy, reduce histomorphological changes and increase the expression of E-cadherin in the colon. Furthermore, the expression levels of TNF-α, IL-6 and NO in the CSO/Dex/LNP-treated group were 37.4 %, 35.5 % and 33.2 % of those in mice with colitis, respectively.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2018
·
IF 2.297
Suping Qiu,Desheng Liang,Feng Guo,Tiantian Deng,Taoxing Peng,Yuan Gao,Xiuying Zhang,Haijun Zhong
In order to reduce enzymatic degradation and thereby improve the stability of solid lipid nanoparticles (SLNs) in the gastrointestinal tract (GIT), two comb-shaped amphiphilic macromolecular materials of N-stearyl-N-trimethyl chitosan (STMC) and N-linoleoyl-N-trimethyl chitosan (LTMC) were fabricated as emulsifier to modify SLNs. Some influence factors of lipolysis medium were investigated to establish in vitro lipolysis model for SLNs. The results showed that the lipolysis curves were fluctuant and variable when the amounts of SLNs were low. The lipolysis of SLNs increased with pancreatic enzyme activity ascending from 150 to 450 USPU/mL and achieved a threshold between 450 and 600 USPU/mL. The lipolysis of STMC-SLNs increased with the initial calcium concentration rising from 0 to 1.4 mM, but had no obvious change from 1.4 to 5 and 10 mM. Besides, SLNs lipolysis increased with bile acid salt and phospholipid concentration increasing. Finally, SLNs stability in GIT was tested with the optimized in vitro lipolysis model. The results revealed that as compared to P188-SLNs and Tween-SLNs, STMC-SLNs lipolysis decreased by 27.33% and 48.55%, and LTMC-SLNs decreased by 22.64% and 43.86%, respectively. Besides, the drug precipitations for STMC-SLNs and LTMC-SLNs were significantly decreased. These results demonstrated that both STMC-SLNs and LTMC-SLNs had excellent gastrointestinal stability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Jianying Huang,Yuhong Liu,Lijun Yang,Fengyan Zhou
A sulfonated chitosan (SCS) was prepared via nucleophilic substitution and characterized by Fourier transform infrared spectroscopy, 1 H and 13 C NMR spectra, gel permeation chromatography, elemental analysis, and thermo gravimetric analysis. The inhibition activities of bacterial adhesion and biofilm formation against E. coli and S. aureus of SCS were assessed in comparison with those of unmodified chitosan hydrochloride (WCS) which was commercially available. The metabolic activity and secretion of exopolysaccharide in biofilms of E. coli and S. aureus were significantly decreased after the treatment of SCS. Scanning electron microscopy and confocal laser scanning microscopy also demonstrated that SCS and WCS at 1MIC concentrations could obviously inhibit the formation of biofilm. Upon the experimental data obtained, it can be concluded that the alkylsulfonation of chitosan could significantly improve efficacy in killing biofilm-embedded bacteria, and the inhibition activities against biofilm formation of E. coli and S. aureus . The exploitation of SCS in this study is helpful to extend the understanding to an alternative to antibiotics and chemical preservatives in food and medicine fields.
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International Journal of Polymer Science
·
2017
·
IF 1.077
Yunzhen Yao,Ding Ding,Hongyuan Shao,Qifan Peng,Yaqin Huang
Fish gelatin-chitosan edible films with D-limonene were successfully prepared, which exhibited exceptional mechanical properties and antimicrobial activity. It has been demonstrated that water-soluble chitosan, fish gelatin, and D-limonene could be a candidate precursor to prepare low cost and high-performance edible food packaging material. The results showed that D-limonene in the films could effectively resist the penetration of light and water because of its hydrophobicity. Moreover, the elongation at break (EAB) increased with the addition of D-limonene, which indicated that D-limonene served as a strong plasticizer for the film. Microscopic characterization showed that D-limonene was uniformly distributed in the as-prepared film. And we found that the film exhibited strong antibacterial activity against Escherichia coli (E. coli). All the results indicate that the as-prepared film could be a promising food packaging.
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Heliyon
·
2022
·
IF 3.776
Suwei Jiang,Hailiang Hu
In this paper, we explore the adsorption potential of catechin (CT) loaded composite microspheres and provide a new micron scale carrier of functional factor. Chitosan (CS) modified rice porous starch (RPS/CS) was used as a CT adsorption carrier to prepare bioactive CT-loaded composite microspheres (CT@RPS/CS). The adsorption kinetics, storage characteristics, and biological activity maintenance of CT@RPS/CS were studied in an aqueous solution, and the sustained-release characteristics of CT@RPS/CS were studied in vitro during simulated gastrointestinal digestion. An aqueous solution further studied the removal characteristics of adsorbed heavy metal ion Pb 2+ . RPS/CS can significantly improve the ability to adsorb CT. RPS/CS can also significantly improve CT's storage stability, antioxidant stress, and slow-release characteristics, and the sustained release effect in gastric and intestinal juice. CT@RPS/CS can be removed Pb 2+ by adsorbing in the solution, and their adsorption was physical adsorption and chemisorption, but the primary interaction is chemisorption. CT@RPS/CS can be used as a micron carrier of new food functional factors, which has potential space for improving and expanding the functional characteristics of its loaded functional factors and the endowing of new functions.
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CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Qing Wan,Meiying Liu,Dazhuang Xu,Liucheng Mao,Jianwen Tian,Hongye Huang,Peng Gao,Fengjie Deng,Xiaoyong Zhang,Yen Wei
Chitosan based nanomaterials have been extensively examined for biomedical applications for their biodegradability, low toxicity, biological activity and low cost. In this work, a novel strategy for fabrication of luminescent polymeric nanoparticles (LPNs) based on aggregation induced emission (AIE) dye and water soluble chitosan (WS-Chitosan) were firstly developed via a highly efficient mercaptoacetic acid (MA) locking imine reaction. In this multicomponent reaction (MCR), MA serves as “lock” to connect 9,10-Bis(aldehydephenl)anthracene dye (An-CHO) and amino-containing WS-Chitosan under mild reaction conditions. The obtained WS-Chitosan@An-CHO LPNs show strong yellow emission and great water dispersibility. Biological evaluation results demonstrated that synthetic luminescent polymeric nanoparticles possess desirable cytocompatibility and distinct imaging properties. Therefore, we have developed a facile and useful method to fabricate AIE active nanoprobes with desirable properties for various biomedical applications. This strategy should be a general and easy handling tool to fabricate many other AIE dye based materials.
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JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Nan Li,Zhi Wang,Jixiao Wang
Developing efficient membrane technology for CO 2 capture has aroused wide interest for environment conservation and emission reduction. Developing facilitated transport membranes is a potential strategy for improving CO 2 permeance and CO 2 /N 2 selectivity synchronously, based on the reversible reactions among carriers and CO 2 molecules. The reaction activity can be optimized by tuning the carrier mobility due to the enhanced water-swelling capacity. Here, thin-film composite (TFC) membranes containing water-swelling carboxymethyl chitosan (CMC) chains were prepared via interfacial polymerization process, aiming to improve CO 2 separation performance. As-prepared TFC membranes with different CMC loading have exhibited different Turing nanostructures , such as corrugated structures, octopus-branched structures and nodular structures. Water can enhance the activity of carriers in reversible reaction for facilitated transport membranes. TFC membranes with CMC have exhibited the enhanced water-swelling capacity, showing high CO 2 permeance of 1278 GPU together with CO 2 /N 2 selectivity of 89. This work put forward a simple and effective method to tune the membrane structure and enhance the facilitated transport.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2012
·
IF 1.289
Di Zhao,JianFeng Xu,Lu Wang,JianJia Du,KeDong Dong,CaiFei Wang,XiangDong Liu
Two phosphorylated chitosan derivatives with a similar degree of substitution of about 0.5 were synthesized via either the esterification of chitosan's hydroxyl groups by P 2 O 5 in methanesulfonic acid or the selective incorporation of methylene phosphonic groups on chitosan's amino groups by formaldehyde/H 3 PO 3 in water. The performances of the resulting O -phosphorylated chitosan (OPC) and N -methylene phosphonic chitosan (NMPC) as amphoteric flocculants were assessed in comparison with that of unmodified chitosan. In turbidity removal experiments using kaolin suspensions in water, OPC removed about 90% turbidity, but NMPC only removed 60%. The lower flocculation efficiency of NMPC was attributed to the decreased acid strength of the N -methylene phosphate groups in NMPC. These results revealed that the acidity of the phosphate groups introduced in the modified chitosans is an important factor with regard to the flocculation efficiency, and consequently that O-phosphorylation is more suitable than N-phosphorylation for preparing chitosan-based amphoteric flocculants. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
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