JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
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2023
·
IF 6
Ruizhe Chen,Yongfeng Liu,Chenyang Yin,Long Wang,Lu Zhang,Jinou Song,Hua Sun
To investigate the influence of 1-nitropropane (1-NP) initiator on the pyrolysis of n-hexane ( n -C 6 H 14 ), molecular dynamics (MD) simulations and synchrotron vacuum ultraviolet photoionization mass spectrometry (SVUV-PIMS) experiments were used to analyze the pyrolysis processes of n -C 6 H 14 and n -C 6 H 14 with 10 % 1-NP added. ReaxFF MD simulations were used to analyze the pyrolysis rates and product distributions of n -C 6 H 14 and 1-NP/ n -C 6 H 14 at different temperatures. The mechanism of action of n -C 6 H 14 pyrolysis initiated by 1-NP was investigated. Additionally, the pyrolysis products of n -C 6 H 14 and 1-NP/ n -C 6 H 14 were identified and quantitatively analyzed using the SVUV-PIMS technique. These experimental results were compared with the ReaxFF MD simulation results to validate the reliability of the simulations and to complement each other. The results indicate that the presence of 1-NP promotes the pyrolysis of n -C 6 H 14 and significantly improves the chemical heat sink of the fuel. The chemical heat sink in the 1-NP/ n -C 6 H 14 system differs from that in the n -C 6 H 14 system by 28.78 kJ/kg at 2103 K. A significant increase in C 2 H 4 production in the pyrolysis products of n -C 6 H 14 with the addition of 1-NP. The pyrolysis of 1-NP/ n -C 6 H 14 yielded approximately twice as much C 2 H 4 as n -C 6 H 14 at 2103 K. The pyrolysis reactions were described by first-order kinetics, and the activation energy for the pyrolysis of 1-NP/ n -C 6 H 14 was determined to be 140 kJ/mol, approximately 48 % lower than that of n -C 6 H 14 . The composition of the pyrolysis products was confirmed by SVUV-PIMS experiments, which were in good agreement with the ReaxFF MD simulations. The mechanism of pyrolysis of n -C 6 H 14 initiated by 1-NP was elucidated: the NO 2 radicals generated by 1-NP react with H radicals of the system to form OH radicals. The primary reaction of n -C 6 H 14 involves the abstraction of H by OH, leading to the formation of C 6 H 13 . Subsequently, C 6 H 13 is further decomposed to form 1-C 3 H 7 , 1-C 4 H 8 , 1-C 5 H 10 and 2-C 6 H 12 .
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2020
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IF 3.263
Cui Zhai,Wei Feng,Wenhua Shi,Jian Wang,Qianqian Zhang,Xin Yan,Qingting Wang,Shaojun Li,Lu Liu,Yilin Pan,Yanting Zhu,Limin Chai,Cong Li,Pengtao Liu,Yuqian Chen,Manxiang Li
It has been shown that sphingosine-1-phosphate (S1P) is elevated in patients with pulmonary arterial hypertension (PAH) and promotes the proliferation of pulmonary artery smooth muscle cells (PASMCs). Meanwhile, S1P has been found to induce the activation of autophagy in several types of human diseases including cancers. However, it is still unclear whether activation of autophagy mediates S1P-induced PASMCs proliferation, and detailed mechanisms responsible for these processes are indefinite. The aims of this study are to address these issues. S1P dose- and time-dependently reduced the expression of E-cadherin/CDH1 and stimulated PASMCs proliferation; this was accompanied with the elevation of TNF receptor-associated factor 2 (TRAF2), up-regulation and ubiquitination of BECN1 and the activation of autophagy. Prior silencing TRAF2 or BECN1 using siRNA or pre-incubation of cells with autophagy inhibitor chloroquine phosphate (CQ) suppressed S1P-induced autophagy activation and subsequent CDH1 degradation and further PASMCs proliferation. Taken together, our study indicates that S1P promotes the activation of autophagy by accelerating TRAF2-mediated BECN1 up-regulation and ubiquitination, which in turn results in CDH1 reduction and contributes to PASMCs proliferation.
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Vaccines
·
2020
·Vol. 8
·期 4
·726
·
IF 4.086
Muhammad Waqqas Hasan,Muhammad Haseeb,Muhammad Ehsan,Javaid Ali Gadahi,Muhammad Ali-ul-Husnain Naqvi,Qiang Qiang Wang,Xinchao Liu,Shakeel Ahmed Lakho,Ruofeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li
ADP-ribosylation factor 1 (HcARF1) is one of theHaemonchus contortus(H. contortus) excretory/secretory proteins involved in modulating the immune response of goat peripheral blood mononuclear cells (PBMC). Here, we evaluated the immunogenic potential of recombinant HcARF1 (rHcARF1) againstH. contortusinfection in Institute of Cancer Research (ICR) mice. Briefly, rHcARF1 was entrapped in poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NP) and injected into mice as a vaccine. Fifty-six ICR mice were assigned randomly into seven groups, with eight animals in each group, and they were vaccinated subcutaneously. At the end of the experiment (14th day), the blood and the spleen were collected from euthanized mice to detect lymphocyte proliferation, cytokine analysis, and the production of antigen-specific antibodies. Scanning electron microscope was used to determine the size, morphology, and zeta potential of nanoparticles. Flow cytometry was performed, which presented the increase percentages of CD4+T cells (CD3e+CD4+), CD8+T cells (CD3e+CD8+) and dendritic cells (CD11c+CD83+, CD11c+CD86+) in mice vaccinated with rHcARF1+PLGA NP. Immunoassay analysis show raised humoral (Immunoglobulin (Ig)G1, IgG2a, IgM) and cell-mediated immune response (Interleukin (IL)-4, IL-12, and IL-17, and Interferon (IFN)-γ) induced by rHcARF1+PLGA NP. Experimental groups that were treated with the antigen-loaded NP yield higher lymphocyte proliferation than the control groups. Based on these results, we could propose that the rHcARF1 encapsulated in NP could stimulate a strong immune response in mice rather than administering alone against the infection ofH. contortus.Keywords:Haemonchus contortus;rHcARF1;PLGA;CS;nanoparticle;ICR mice
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JOURNAL OF THE AMERICAN CERAMIC SOCIETY
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2023
·Vol. 107
·期 5
·3219-3229
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IF 3.9
Hongtao Li,Rongxia Huang,Hua-Tay Lin,Zuchao Du,Yejing Dai
This research investigated the effect of Bi 4 Ti 3 O 12 (BiT) template particles size on the structure and piezoelectric properties of 〈001〉-textured (Bi 0.5 Na 0.5 )TiO 3 (BNT)-based lead-free piezoelectric ceramics. Results of X-ray diffractometer analyses and scanning electron microscopy observations showed that high purity BiT template particles could be obtained with the KCl as the molten salt system. Highly 〈001〉-textured (~87%) BNT-based ceramics prepared with large size BiT templates (7.75 µm) exhibited a high inverse piezoelectric constant d 33 * (1100 pm/N), which was ∼3.38-fold larger than that with 1.91 µm (325 pm/N). In addition, we have found that too small the template size can make it difficult to align the orientation of the template during the casting process, whereas too large the size can create problems such as too much hysteresis (∆ S / S max = 67%). This study has demonstrated that the use of high purity large size BiT template particles could provide a feasible route to fabricate BNT-based textured ceramics with desired electro-strain properties.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Juntao Tang,Mengying Fu,Yidan Mao,Shuyi Yang,Wei Lin,Yan Yu,Shuang Song
Photocatalysis is regarded as a promising in situ air purification technology for the removal of volatile organic compounds. However, the deactivation of photocatalysts remains a serious issue that needs to be addressed. Herein, we report a novel dual-functional photocatalyst (TiO 2 @HYZ) with spatially separated catalytic sites and adsorption sites, which has been obtained by combining hydrophilic {0 0 1} TiO 2 nanosheets with hydrophobic NaY zeolite. TiO 2 @HYZ showed excellent photocatalytic activity for the degradation of gaseous toluene under UV irradiation in humid air. The optimized TiO 2 @HYZ-2 (containing 45.7 wt% TiO 2 ) achieved 96.6% toluene removal and 87.4% mineralization within 120 min. The specific reaction rate constant of TiO 2 @HYZ-2, pristine TiO 2 and P25 normalized by the unit amount of TiO 2 during the photocatalytic degradation process were calculated to be 0.481, 0.230 and 0.250 min −1 g −1 , respectively. Moreover, TiO 2 @HYZ-2 showed superior durability, maintaining stable performance over repeated toluene degradation processes, even at high toluene concentration, whereas pristine TiO 2 incurred severe deactivation with increasing number of photocatalysis cycles. The significantly enhanced activity and anti-deactivation property was attributed to a synergistic effect between HYZ and TiO 2 in the catalyst structure, which strengthened the adsorption of toluene on HYZ and facilitated effective separation of photoexcited charge carriers on TiO 2 , thus avoiding the deposition of degradation intermediates on the active sites and simultaneously improving mineralization. This work provided a new perspective for the design of efficient and deactivation-resistant photocatalysts for air pollution remediation.
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CNS Neuroscience & Therapeutics
·
2023
·
IF 5.5
Tao Zhang,Yuan Tian,Xiaoqing Zheng,Ruomeng Li,Li Hu,Xindong Shui,Yingxue Mei,Quling Wang,Mi Zhang,Xiuzhi Zheng,Long Wang,Dongmei Chen,Wucheng Tao,Tae Ho Lee
Aims The autophagy-lysosomal pathway is important for maintaining cellular proteostasis, while dysfunction of this pathway has been suggested to drive the aberrant intraneuronal accumulation of tau protein, leading to synaptic damage and cognitive impairment. Previous studies have demonstrated that the activation of transient receptor potential vanilloid 1 (TRPV1) by capsaicin has a positive impact on cognition and AD-related biomarkers. However, the effect and mechanism of TPRV1 activation on neuronal tau homeostasis remain elusive. Methods A mouse model of tauopathy was established by overexpressing full-length human tau in the CA3 area. Mice were fed capsaicin diet (0.0125%) or normal diet for 9 weeks. The cognitive ability, synaptic function, tau phosphorylation levels, and autophagy markers were detected. In vitro, capsaicin-induced alterations in cellular autophagy and tau degradation were characterized using two cell models. Besides, various inhibitors were applied to validate the role of TRPV1-mediated autophagy enhancement in tau clearance. Results We observed that TRPV1 activation by capsaicin effectively mitigates hippocampal tau accumulation-induced synaptic damages, gliosis, and cognitive impairment in vivo. Capsaicin promotes the degradation of abnormally accumulated tau through enhancing autophagic function in neurons, which is dependent on TRPV1-mediated activation of AMP-activated protein kinase (AMPK) and subsequent inhibition of the mammalian target of rapamycin (mTOR). Blocking AMPK activation abolishes capsaicin-induced autophagy enhancement and tau degradation in neurons. Conclusion Our findings reveal that capsaicin-induced TRPV1 activation confers neuroprotection by restoring neuronal tau homeostasis via modulating cellular autophagy and provides additional evidence to support the potential of TRPV1 as a therapeutic target for tauopathies.
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JOURNAL OF NATURAL PRODUCTS
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2023
·
IF 5.1
Jinghan Zhang,Xiao Xu,Yu Zhao,Chunling Ren,Mengzhen Gu,Haili Zhang,Peiye Wu,Yun Wang,Lingyi Kong,Chao Han
The glucose transporter 1 (GLUT1) protein is involved in the basal-level absorption of glucose in tumor cells. Inhibiting GLUT1 decreases tumor cell proliferation and induces tumor cell damage. Natural GLUT1 inhibitors have been studied only to a small extent, and the structures of known natural GLUT1 inhibitors are limited to a few classes of natural products. Therefore, discovering and researching other natural GLUT1 inhibitors with novel scaffolds are essential. Physalis angulata L. var. villosa is a plant known as Mao-Ku-Zhi (MKZ). Withanolides are the main phytochemical components of MKZ. MKZ extracts and the components of MKZ exhibited antitumor activity in recent pharmacological studies. However, the antitumor-active components of MKZ and their molecular mechanisms remain unknown. A cell membrane-biomimetic nanoplatform (CM@Fe3O4/MIL-101) was used for target separation of potential GLUT1 inhibitors from MKZ. A new withanolide, physagulide Y (2), together with six known withanolides (1, 3–7), was identified as a potential GLUT1 inhibitor. Physagulide Y was the most potent GLUT1 inhibitor, and its antitumor activity and possible mechanism of action were explored in MCF-7 human cancer cells. These findings advance the development of technologies for the targeted separation of natural products and identify a new molecular framework for the investigation of natural GLUT1 inhibitors.
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Biochemistry and Biophysics Reports
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2023
·
IF 2.7
Ke Zhang,Youyang Liu,Yunlu Zhao,Qi Guo,Shengjun An,Shuhui Wu
Many chronic inflammatory diseases, such as autoimmune inflammation, are associated with M1 macrophages, and the key to their treatment is blocking inflammation. Oxymatrine (OMT), a traditional Chinese medicine, has a marked anti-inflammatory effect. However, its anti-inflammatory target and mechanism in M1 cells remain unclear, which limits its clinical application. In this study, we investigated the anti-inflammatory effects of oxymatrine (OMT) on the M1 inflammatory response. We also determined the relationship between OMT treatment and the nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) pathway with OMT treatment. To this end, we induced the differentiation of human peripheral blood monocytes (THP-1) into M1 cells. THP-1 cells were induced with a phorbol ester (phorbol-12-myristate-13-acetate (PMA)) and differentiated into naïve M0 macrophages. M0 cells were induced into M1 cells using lipopolysaccharide (LPS). The experimental groups were divided into the M0 macrophage group (NC), M1 inflammatory response group (LPS group), and M1 group treated with different concentrations of OMT (LPS + OMT-L, LPS + OMT-M, LPS + OMT-H). The cells in the OMT-treated groups were treated with OMT for 6 h, followed by LPS for 24 h, and the LPS group was treated with LPS only. The resulting supernatants and cells were collected. The secretion levels of NO were detected by the Griess method and the secretion levels of TNF-α and IL-1β in the supernatants were detected by the ELISA method. The secretion levels of these inflammatory factors were reduced in every OMT-treated group compared to the LPS group (P < 0.01), and the most significant reductions were found in the OMT-H group (P < 0.0001). By western blotting, the protein expression levels of TLR4, NF-κB, NLRP3, and Caspase-1 were all found to be downregulated in the cells of OMT-treated groups compared to the LPS group (P < 0.0001). In situ changes in NLRP3 expression were observed using immunofluorescence. The fluorescence intensity of NLRP3 in M1 cells was weaker in all OMT intervention groups than in the LPS group (P < 0.001). In conclusion, OMT has significant anti-inflammatory effects on the M1 inflammatory responses, and the TLR4/NF-κB/NLRP3 pathway was blocked proportional to the concentration of OMT.
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JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2023
·
IF 6.1
Lei Jin,Zhao-Yun Wang,Zhuan-Yun Cai,Jia-Qiang Yang,An-Ni Zheng,Fang-Zu Yang,De-Yin Wu,Dongping Zhan
In this work, we develop a novel 1-(2-Pyridylazo)-2-naphthol (PAN)-based acidic sulfate copper electronic electroplating process for compact, homogeneous and conformal copper thickening of through hole (TH). The adsorption characteristics, electrochemical properties and the interaction mechanisms of three additives (PEG, SPS and PAN) are studied in detail. Scanning electron microscope (SEM) and optical microscope (OM) reveal that PAN can make the copper coating in fine grains and high throwing power of 90.35% in TH without an overhang phenomenon. Theoretical calculations illustrate that the binding energies between additive molecules and copper surface decrease in the order: PAN > SPS > PEG . Cyclic voltammetry experiments at rotating rates of 1200 and 200 r/min prove that PAN itself can accelerate and weakly suppress copper electrodeposition at the center and mouth of TH, respectively. PAN behaves respectively the synergistic and antagonistic effects with PEG at the mouth and center of TH, and the weakly synergistic effects with SPS both at the mouth and center of TH. The addition of PAN to the virgin make-up solution (VMS) containing PEG and SPS causes decreasing the relative electric charge (Δ Q ) at high and low rotating rates from 18.52 ± 0.08% to 8.25 ± 0.05%, which satisfies the closer copper electronic electroplating rate at the center and mouth of TH. The effective and complicated synergistic effects of the additives PEG, SPS and PAN can achieve compact, homogeneous and conformal copper thickening of TH.
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Journal of Pharmaceutical Analysis
·
2023
·
IF 8.8
Zhang Mao,Haochen Hui,Xuerong Zhao,Lina Xu,Yan Qi,Lianhong Yin,Liping Qu,Lan Han,Jinyong Peng
It is necessary to explore potent therapeutic agents via regulating gut microbiota and metabolism to combat Parkinson's disease (PD). Dioscin, a bioactive steroidal saponin, shows various activities. However, its effects and mechanisms against PD are limited. In this study, dioscin dramatically alleviated neuroinflammation and oxidative stress, and restored the disorders of mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). 16S rDNA sequencing assay demonstrated that dioscin reversed MPTP-induced gut dysbiosis to decrease Firmicutes -to- Bacteroidetes ratio and the abundances of Enterococcus , Streptococcus , Bacteroides and Lactobacillus genera , which further inhibited bile salt hydrolase (BSH) activity and blocked bile acid (BA) deconjugation. Fecal microbiome transplantation test showed that the anti-PD effect of dioscin was gut microbiota-dependent. In addition, non-targeted fecal metabolomics assays revealed many differential metabolites in adjusting steroid biosynthesis and primary bile acid biosynthesis. Moreover, targeted bile acid metabolomics assay indicated that dioscin increased the levels of ursodeoxycholic acid, tauroursodeoxycholic acid, taurodeoxycholic acid and β-muricholic acid in feces and serum. In addition, ursodeoxycholic acid administration markedly improved the protective effects of dioscin against PD in mice. Mechanistic test indicated that dioscin significantly up-regulated the levels of takeda G protein-coupled receptor 5 (TGR5), glucagon-like peptide-1 receptor (GLP-1R), GLP-1, superoxide dismutase (SOD), and down-regulated NADPH oxidases 2 (NOX2) and nuclear factor-kappaB (NF-κB) levels. Our data indicated that dioscin ameliorated PD phenotype by restoring gut dysbiosis and regulating bile acid-mediated oxidative stress and neuroinflammation via targeting GLP-1 signal in MPTP-induced PD mice, suggesting that the compound should be considered as a prebiotic agent to treat PD in the future.
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JOURNAL OF CATALYSIS
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2023
·
IF 7.3
Yuexia Wang,Qibin Yao,Bowen Xu,Kehang Lin,Mengshan Deng,Xinqing Lu,Rui Ma,Yanghe Fu,Weidong Zhu
Although the oxidative hydration of ethylene with hydrogen peroxide (H 2 O 2 ) to ethylene glycol (EG) over Ti-MWW zeolites take advantages of step-saving and facile operation, both H 2 O 2 utilization efficiency and EG yield are relatively low, because some extra-framework octahedral Ti species are usually formed in the Ti-MWW zeolites synthesized by prevailing methods, which are detrimental to selective oxidation . In the present work, an acid-modulated isomorphous substitution method, i.e. , layered borosilicate MWW-type zeolite (ERB-1) treated with HNO 3 aqueous solution containing tetrabutyl orthotitanate (TBOT), is developed to synthesize a Ti-MWW zeolite without extra-framework octahedral Ti species under the optimized synthesis conditions. In combination with the results from extensive characterizations for the synthesized zeolites, it is found that in the zeolite prepared by the currently developed synthesis method there are more open Ti(OSi) 3 OH sites formed, resulting in the stronger Lewis acidity that is very helpful to EG production from the oxidative hydration of ethylene with H 2 O 2 , compared to the one synthesized by the prevailing method. Moreover, the developed catalyst is recyclable after the regeneration of the deactivated zeolite by calcination at high temperatures. Therefore, it is anticipated that the currently developed Ti-MWW might be industrially applicable in the one-pot synthesis of EG from ethylene with H 2 O 2 .
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Advanced Healthcare Materials
·
2023
·
IF 10
Ya Ren,Weiqing Kong,Yihao Liu,Xue Yang,Xiang Xu,Lei Qiang,Xuelian Mi,Changru Zhang,Haoyi Niu,Chengwei Wang,Jinwu Wang
Bone defect repair remains a major clinical challenge that requires the construction of scaffolds that can regulate bone homeostasis. In this study, a photo-cured mesoporous bioactive glass (PMBG) precursor is developed as a tricalcium phosphate (TCP) agglomerant to obtain a double-phase PMBG/TCP scaffold via 3D printing. The scaffold exhibits multi-scale porous structures and large surface areas, making it a suitable carrier for the loading of parathyroid hormone (PTH) (1-34), which is used for the treatment of osteoporosis. In vitro and in vivo results demonstrate that PMBG/TCP scaffolds coordinated with PTH (1-34) can regulate bone homeostasis in a bidirectional manner to facilitate bone formation and inhibit bone resorption. Furthermore, bidirectional regulation of bone homeostasis by PTH (1-34) is achieved by inhibiting fibrogenic activation protein (FAP). Thus, PMBG/TCP scaffolds coordinated with PTH (1-34) are viable materials with considerable potential for application in the field of bone regeneration and provide an excellent solution for the design and development of clinical materials.
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危险品化学品经营许可证（带存储）