JOURNAL OF INORGANIC BIOCHEMISTRY
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2020
·
IF 3.212
Han-Long Cheng,Hai-Ling Guo,An-Jian Xie,Yu-Hua Shen,Man-Zhou Zhu
At present, cancer has become a major disease threatening human health worldwide. Therefore, developing targeting guided multimode synergetic therapy has become one of the hot spots in current antitumor research and is also a great challenge. Herein, a new Fe 3 O 4 /g-C 3 N 4 @PPy-DOX nanocomposite containing magnetic iron oxide (Fe 3 O 4 ) nanoparticles (NPs), lamellar structure of graphite-like carbon nitride (g-C 3 N 4 ) and polypyrrole (PPy) shell with the loaded anti-tumor drug doxorubicin hydrochloride (DOX) was designed and prepared. The monodisperse Fe 3 O 4 nanoparticles (NPs) with the diameter of 20 nm endowed the nanocomposite with the magnetic targeting ability, reducing damage to normal tissues. It is very interesting that the Fe 3 O 4 NPs also possessed photosensitizer function for photodynamic therapy (PDT). The g-C 3 N 4 sheets as the photocatalysis towards the degradation of water for generating O 2 could effectively improve the hypoxia of solid tumors and increase the efficiency of PDT. In addition, PPy has high light-to-heat conversion efficiency, so was chosen for the cancer photothermal therapy (PTT). Finally, an anticancer drug (DOX) was loaded on the nanocomposite because the presence of mesoporous structure. Thus, the prepared Fe 3 O 4 /g-C 3 N 4 @PPy-DOX nanocomposites exhibit synergetic chemotherapy/PTT/enhanced PDT antitumor effect. This study provides an inspiration for combining targeting and multimodality to improve the anticancer efficiency.
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高影响因子
DIGESTIVE DISEASES AND SCIENCES
·
2020
·Vol. 66
·期 10
·3375-3390
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IF 2.751
Zhang Yu,Li Xiaozhi,Li Yaoting,Li Ying,Wang Ying,Zhu Liguo,Chen Peng,Tian Zhenyi,Qiu Yun,Feng Rui,Zeng Zhirong,He Yao,Chen Baili,Zhang Shenghong,Mao Ren,Chen Minhu
Background and Aims DNA damage-regulated autophagy modulator 1 (DRAM1) is required for induction of autophagy and apoptosis. However, the influence of DRAM1 on the pathogenesis of inflammatory bowel disease (IBD) has not been explored. Methods DRAM1 expression was examined in the intestinal mucosa of patients with IBD and colons of colitis mice. We used a recombinant adeno-associated virus carrying small hairpain DRAM1 to knock down the DRAM1 gene to treat colitis in the mice. The effect of DRAM1 on autophagy and apoptosis of intestinal epithelial cells was explored. DRAM1-mediated interaction with the c-Jun N-terminal kinase (JNK) pathway was also examined. Results DRAM1 expression in the intestinal mucosa of the IBD patients was higher than that in the control participates. DRAM1 expression in the inflammatory cells in patients with Crohn’s disease (CD) was lower than that in patients with ulcerative colitis (UC). Additionally, DRAM1 expression was correlated with the Simple Endoscopic Score for CD and the Mayo endoscopic score for UC. Serum levels of DRAM1 in the IBD group were substantially higher than those in the normal group. The knockdown of DRAM1 could alleviate colitis symptoms in mice. In in vitro experiments, knocking down DRAM1 could reduce autophagy and apoptosis levels. Mechanistically, DRAM1 may participate in the regulation of these two processes by positively regulating JNK activation. Conclusions During intestinal inflammation, the upregulation of DRAM1 may promote the activation of JNK and further aggravate intestinal epithelium damage.
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JOURNAL OF CELLULAR PHYSIOLOGY
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2020
·Vol. 236
·期 3
·1889-1902
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IF 5.546
Wenhui Yao,Kai Wang,Xiniao Wang,Xinran Li,Jieyan Dong,Yusheng Zhang,Xuansheng Ding
Icariin (ICA), as a flavonoid glycoside, is associated with the improvement of vascular complications in diabetes. However, its protective mechanisms remain to be well-established. Here, we tested the hypothesis that ICA attenuates vascular endothelial dysfunction by inhibiting endoplasmic reticulum (ER) stress in type 1 diabetes. In streptozotocin-induced diabetic rats, ICA positively affected acetylcholine-induced vasodilation and phenylephrine-induced vasoconstriction in aortas. ICA treatment significantly attenuated ER stress in diabetic rats and high-glucose induced human umbilical vein endothelial cells. Incubation with ICA in vitro attenuated vascular reactivity in diabetic rats, which was blocked by the ER stress inducer, and peroxisome proliferator-activated receptor α (PPARα), sirtuin1 (Sirt1), or AMP-activated protein kinase-α (AMPKα) inhibitors. Western blot showed that ICA activated the PPARα/Sirt1/AMPKα pathway, which contributed to reducing ER stress and activating endothelial nitric oxide synthase in vivo and vitro. Our results implicate that ICA normalizes ER stress to attenuate endothelial dysfunction by the regulation of the PPARα/Sirt1/AMPKα pathway.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yuqiang Hu,Zhen Zhang,Weicong Ye,Wei Zhang,Minghao Hu,Wenqian Yuan,Hongbo Wang,Xianjin Xiao,Tongbo Wu
Human apurinic/apyrimidinic endonuclease 1 (APE1) plays a crucial role in DNA repair and gene regulation. The abnormal variations of the concentration of APE1 in the human blood and tissue cells are highly correlated to various diseases. Thus, APE1 can be used as a biomarker to aid clinical diagnosis of diseases. Detection of APE1 activity with high sensitivity and selectivity simultaneously is difficult to achieve. Here we provide a DNA structure-mediated fluorescent biosensor to solve the above problem. Upon the existence of APE1, the apurinic/apyrimidinic site will be cleaved, and the terminal structure of the hairpin DNA substrate will change. Then the terminal deoxynucleotidyl transferase and endonuclease IV could provide dual fluorescent signal amplification. Through the ingenious structure design of the biosensor, we have improved the reactivity of APE1 and minimized the interference of other enzymes. The results indicate that the detection limit for APE1 is as low as 1.7 × 10 −6 U/mL. The biosensor has been successfully applied to the detection of APE1 in real biological samples and the screening of APE1 inhibitors.
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Ying Zhang,Wanliang Liao,Yuanyuan Dai,Weiping Wang,Aijun Wang
In this work, a covalent organic framework Schiff base network-1 (SNW-1), was synthesized based on the Schiff base reaction between terephthalaldehyde and melamine and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and nitrogen adsorption-desorption isotherm analyses. The prepared SNW-1 was employed as pipette tip solid phase extraction adsorbent for the extraction of sulfonamides (SAs) prior to high performance liquid chromatography analysis. The parameters affecting the extraction efficiency, including the salt concentration, sample pH, amount of adsorbent, and types and volume of eluent were investigated in detail. Good linearities were obtained between the peak area and SAs concentration ranging from 5 to 500 ng mL −1 with correlation coefficients (R 2 ) higher than 0.9998. The limits of detection and RSDs were lower than 0.25 ng mL −1 and 1.9 %, respectively. The developed method was further applied for the determination of SAs in milk and honey samples with recoveries in the range of 85.8 % – 118.0 % and RSDs less than 9.5 %. The results demonstrate that the SNW-1 shows great potential for the enrichment of trace SAs in complex matrices.
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Jiating Zheng,Shuyao Huang,Yuanjun Tong,Songbo Wei,Guosheng Chen,Siming Huang,Gangfeng Ouyang
The secretion disorder of sex hormones is the source that leads to the occurrence of many diseases such as polycystic ovarian syndrome (PCOS), hyperandrogenism and so on. There exist physiological changes in human body when slight fluctuations in concentrations of sex hormones happen. Therefore, it’s of great significance for accurate detection of sex hormones in human body. In this work, TpPa-1 COF solid-phase microextraction (SPME) fiber was prepared using high-efficient in-situ synthesis strategy and coupled with HPLC-MS/MS to detect three sex hormones, including Progesterone (P), testosterone (T) and dehydroepiandrosterone (DHEA) in human serum. The thickness of the coating reached 7 μ m within 2 h. Under the optimal conditions, the established method presented low limit of detections (LODs, ≤ 0.75 ng/mL), wide linear ranges (0.100–100 ng mL −1 ) and good reproducibility, and three sex hormones (T, P, DHEA) were successfully detected and quantified in human serum. In conclusion, the established SPME method presented high-efficient fiber preparation and good analytical performances of sex hormone detection, therefore was in great potential for application in clinical.
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EXPERIMENTAL EYE RESEARCH
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2020
·
IF 3.011
Yuzhen Hou,Jie Lan,Fan Zhang,Xianggen Wu
As a damage-associated molecular pattern molecule, high-mobility group box 1 protein (HMGB1) is involved in diabetes and its complications. However, the role of HMGB1 in diabetic keratopathy is not yet understood. The purpose of this study was to investigate the potential roles of HMGB1 in the development of diabetic keratopathy as well as potential strategies to block HMGB1 in order to prompt epithelial wound healing and nerve regeneration in diabetic corneas. The results demonstrated that diabetic keratopathy developed in mice over the duration of the diabetic condition with typical symptoms, including damaged ocular surfaces and corneal nerves . The diabetic corneas had significantly increased protein expression levels of HMGB1 and its receptors—the receptor for advanced glycation end products (RAGE) and toll-like receptor 4 (TLR4)—compared to the age-matched normal corneas ( P < 0.05). Corneal HMGB1 levels significantly increased during the corneal wound healing process of the diabetic mice, peaking on the first day after the wound was created and then decreasing to the unwounded level on the seventh day. Exogenous HMGB1 peptide significantly retarded wound and nerve healing, while glycyrrhizin (an HMGB1 inhibitor) significantly prompted wound and nerve healing. Further, the western blot results confirmed that RAGE and TLR4 were also involved in corneal wound and nerve healing. In conclusion, these data showed that HMGB1 and its related receptors are highly involved in the development of diabetic keratopathy. This finding indicates that the blockage of HMGB1 might serve as a strategy to prompt diabetic corneal and nerve wound healing.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2020
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IF 3.263
Cui Zhai,Wei Feng,Wenhua Shi,Jian Wang,Qianqian Zhang,Xin Yan,Qingting Wang,Shaojun Li,Lu Liu,Yilin Pan,Yanting Zhu,Limin Chai,Cong Li,Pengtao Liu,Yuqian Chen,Manxiang Li
It has been shown that sphingosine-1-phosphate (S1P) is elevated in patients with pulmonary arterial hypertension (PAH) and promotes the proliferation of pulmonary artery smooth muscle cells (PASMCs). Meanwhile, S1P has been found to induce the activation of autophagy in several types of human diseases including cancers. However, it is still unclear whether activation of autophagy mediates S1P-induced PASMCs proliferation, and detailed mechanisms responsible for these processes are indefinite. The aims of this study are to address these issues. S1P dose- and time-dependently reduced the expression of E-cadherin/CDH1 and stimulated PASMCs proliferation; this was accompanied with the elevation of TNF receptor-associated factor 2 (TRAF2), up-regulation and ubiquitination of BECN1 and the activation of autophagy. Prior silencing TRAF2 or BECN1 using siRNA or pre-incubation of cells with autophagy inhibitor chloroquine phosphate (CQ) suppressed S1P-induced autophagy activation and subsequent CDH1 degradation and further PASMCs proliferation. Taken together, our study indicates that S1P promotes the activation of autophagy by accelerating TRAF2-mediated BECN1 up-regulation and ubiquitination, which in turn results in CDH1 reduction and contributes to PASMCs proliferation.
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Vaccines
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2020
·Vol. 8
·期 4
·726
·
IF 4.086
Muhammad Waqqas Hasan,Muhammad Haseeb,Muhammad Ehsan,Javaid Ali Gadahi,Muhammad Ali-ul-Husnain Naqvi,Qiang Qiang Wang,Xinchao Liu,Shakeel Ahmed Lakho,Ruofeng Yan,Lixin Xu,Xiaokai Song,Xiangrui Li
ADP-ribosylation factor 1 (HcARF1) is one of theHaemonchus contortus(H. contortus) excretory/secretory proteins involved in modulating the immune response of goat peripheral blood mononuclear cells (PBMC). Here, we evaluated the immunogenic potential of recombinant HcARF1 (rHcARF1) againstH. contortusinfection in Institute of Cancer Research (ICR) mice. Briefly, rHcARF1 was entrapped in poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NP) and injected into mice as a vaccine. Fifty-six ICR mice were assigned randomly into seven groups, with eight animals in each group, and they were vaccinated subcutaneously. At the end of the experiment (14th day), the blood and the spleen were collected from euthanized mice to detect lymphocyte proliferation, cytokine analysis, and the production of antigen-specific antibodies. Scanning electron microscope was used to determine the size, morphology, and zeta potential of nanoparticles. Flow cytometry was performed, which presented the increase percentages of CD4+T cells (CD3e+CD4+), CD8+T cells (CD3e+CD8+) and dendritic cells (CD11c+CD83+, CD11c+CD86+) in mice vaccinated with rHcARF1+PLGA NP. Immunoassay analysis show raised humoral (Immunoglobulin (Ig)G1, IgG2a, IgM) and cell-mediated immune response (Interleukin (IL)-4, IL-12, and IL-17, and Interferon (IFN)-γ) induced by rHcARF1+PLGA NP. Experimental groups that were treated with the antigen-loaded NP yield higher lymphocyte proliferation than the control groups. Based on these results, we could propose that the rHcARF1 encapsulated in NP could stimulate a strong immune response in mice rather than administering alone against the infection ofH. contortus.Keywords:Haemonchus contortus;rHcARF1;PLGA;CS;nanoparticle;ICR mice
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JOURNAL OF NUCLEAR MATERIALS
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2020
·
IF 2.485
Yi Ding,Zhengdi Jiang,Tianheng Xiong,Zimei Bai,Dandan Zhao,Hui Dan,Tao Duan
Zircon is important ceramic used widely due to its excellent properties, in particular for nuclear waste immobilization . However, it is difficult to obtain mono-phase zircon with high yield, usually result in double-phase ZrO 2 /ZrSiO 4 ceramics. Herein, Ce-doped double-phase 0.2Zr 1- x Ce x O 2 /Zr 1- y Ce y SiO 4 (0 ≤ x + y ≤ 1) ceramics were fabricated and their phase and microstructure evolution were studied. The results demonstrated that Ce content has great influence on the phase composition and microstructure. The ceramics with 0 ≤ x + y < 0.1 are tetragonal-ZrSiO 4 , monoclinic-ZrO 2 and tetragonal-ZrO 2 phases, with 0.1 ≤ x + y ≤ 0.3 are tetragonal-ZrSiO 4 , monoclinic-ZrO 2 , tetragonal-ZrO 2 and cubic-ZrO 2 phases, with 0.3 < x + y ≤ 0.6 are tetragonal-ZrSiO 4 , tetragonal-ZrO 2 and cubic-ZrO 2 phases, and with 0.6 < x + y ≤ 1 are cubic-ZrO 2 and Ce 2 Si 2 O 7 phases. With increasing Ce-doping content, the ZrSiO 4 retained the tetragonal phase, while ZrO 2 transformed from monoclinic to stable cubic phase. Furthermore, lattice parameters , grain size and compactness increased with increasing Ce content.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2020
·Vol. 31
·期 11
·8489-8495
·
IF 2.22
Shi Liang,Zhang Huaiwu,Shi Xiaolei,Liu Cheng,Peng Rui,Wang Gang,Wang Xueying
Excellent microwave ceramics play a dominant role in current 5G communication technology and Internet of things technology. In current work, high-quality factor of Li 2 Mg 3 TiO 6 - x BaV 2 O 6 (LMT- x BV 2 , x = 0.1, 0.3, 0.4, 0.5, 0.6) ceramics were synthesized via the traditional solid-state reaction method. Phase composition, microstructure, microwave dielectric properties and co-firing compatibility with silver were investigated systematically. The sintering temperature of LMT ceramics was successfully reduced from 1280 °C to below 950 °C with the addition of BV 2 ceramics. In particular, when x value was 0.1, high-quality factor 90,100 GHz (at 10 GHz), a permittivity 14.3 and τ f value − 65 ppm/°C were gained when sintered at 900 °C for 4 h. The linear approximation formula was used to qualitatively illustrate the relationship between microstructure and microwave dielectric properties. The good chemical compatibility of LMT-0.1BV 2 with silver provides a good candidate for LTCC processing.
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RSC Advances
·
2020
·Vol. 10
·期 20
·11643-11651
·
IF 3.119
Qi Xia,Hong Peng,Lin Yuan,Lifang Hu,Yu Zhang,Roger Ruan
The exploration of a highly efficient and environment-friendly solvent for dissolving hemicellulose is significant. In this study, 1-butyl-3-methylimidazolium carboxylate ([Bmim]carboxylate)-based ionic liquids (ILs), including [Bmim]formate, [Bmim]acetate, [Bmim]propionate, and [Bmim]butyrate, were used as solvents to dissolve arabinoxylan-rich hemicellulose from bamboo. The hemicellulose solubility in the ILs was determined as a function of temperature. The interaction between the hemicellulose and the ILs was evaluated by using 1H and 13C NMR techniques. The hemicelluloses regenerated from the saturated IL solutions were characterized. Results showed that the temperature and structure of carboxylate anions deeply affected the hemicellulose solubility. The carboxylate anion played a more important role than the imidazolium cation in hemicellulose dissolution. The hydrogen bond that formed between the ILs and the hydroxyl groups at the XC2 position of xylopyranose units of hemicellulose was stronger than that between the ILs and the hydroxyl groups at XC3 position of xylopyranose units. The hydrogen bond strength between the hemicellulose and the ILs was affected by the alkyl chain of the carboxylate anion and the hemicellulose concentration. The disruption of the inter- and intra-molecular hydrogen bonds in hemicellulose by the ILs was responsible for the hemicellulose dissolution. The main chain of hemicellulose remained nearly unchanged during the dissolution process.
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危险品化学品经营许可证（带存储）