MOLECULAR NEUROBIOLOGY
·
2016
·Vol. 55
·期 1
·421-434
·
IF 5.397
Dai Chongshan,Ciccotosto Giuseppe D.,Cappai Roberto,Tang Shusheng,Li Daowen,Xie Sanlei,Xiao Xilong,Velkov Tony
Neurotoxicity is an unwanted side-effect seen in patients receiving therapy with the “last-line” polymyxin antibiotics. This is the first study to show that colistin-induced neurotoxicity in neuroblastoma-2a (N2a) cells gives rise to an inflammatory response involving the IL-1β/p-IκB-α/NF-κB pathway. Pretreatment with curcumin at 5, 10, and 20 μM for 2 h prior to colistin (200 μM) exposure for 24 h, produced an anti-inflammatory effect by significantly down-regulating the expression of the pro-inflammatory mediators cyclooxygenase-2 (COX-2), phosphorylation of the inhibitor of nuclear factor-kappa B (NF-κB) (p-IκB)-α, and concomitantly NF-κB levels. Moreover, curcumin significantly decreased intracellular reactive oxygen species (ROS) production and increased the activities of the anti-ROS enzymes superoxide dismutase, catalase, and the intracellular levels of glutathione. Curcumin pretreatment also protected the cells from colistin-induced mitochondrial dysfunction, caspase activation, and subsequent apoptosis. Overall, our findings demonstrate for the first time, a potential role for curcumin for treating polymyxin-induced neurotoxicity through the modulation of NF-κB signaling and its potent anti-oxidative and anti-apoptotic effects.
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JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 12
·1-16
·
IF 2.101
Li Zhenzhen,Zhang Qiyi,Huang Huaying,Ren Changjing,Pan Yujin,Wang Qing,Zhao Qiang
In the experiments, high-quality, water-soluble and near-infrared (NIR)-emitting CdSeTe and CdSeTe/CdS quantum dots (QDs) were successfully prepared. The average size of CdSeTe⁄CdS QDs was 7.68 nm and CdSeTe QDs was 4.33 nm. Arginine-glycine-aspartic-serine acid (RGDS) peptides were linked to CdSeTe/CdS QDs by N-(3-(dimethylamino)propyl)-N′-ehtylcarbodiimide hydrochloride (EDC) and N′-hydroxysuccinimide (NHS). The prepared RGDS-tagged NIR CdSeTe/CdS QDs (denoted as RGDS-CdSeTe/CdS) had an average diameter of 24.83 nm and were used for cancer cell immunofluorescence imaging. The characteristics of RGDS-conjugated CdSeTe/CdS such as morphology, structure, spectra, stability, cytotoxicity, and near-infrared microscopic imaging were investigated in detail. HepG2 cells were incubated with the novel fluorescent probe (RGDS-CdSeTe/CdS), which realized immunofluorescence targeting and imaging. The results reported here open up new perspectives for integrin-targeted near-infrared imaging and may aid in tumor detection including imaging-guided surgery.
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2016
·Vol. 21
·期 5
·1297-1303
·
IF 2.327
Zhao Ruopeng,Liu Zhenyuan,Gong Mingxing,Zhang Qingwen,Shi Xinhao,Hu Yongqi,Qi Weiye,Tang Yawen,Wang Yi
The synthesis of Pd nanocrystals of controlled size and morphology has drawn enormous interest due to their catalytic activity. We report a new and efficient strategy for the one-step synthesis of monodispersed Pd nanocubes with ethylenediamine tetramethylene phosphonate (EDTMP) as a complex-forming and capping agent. The morphology, structure, and growth mechanism of the Pd nanocubes were fully characterized via selected area electron diffraction (SAED), transmission electron microscopy (TEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). It was found that the morphology of the Pd nanocrystals in the proposed EDTMP–PdCl 2 system could be changed from octahedrons to nanocubes simply by adjusting the amount of iodide used during synthesis. After UV/ozone and electrochemical cleaning, the as-prepared Pd nanocubes demonstrated excellent electrocatalytic activity and stability during formic acid oxidation, owing to their abundant {100} facets and small particle size.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2016
·Vol. 182
·期 2
·482-494
·
IF 1.606
Bai Yin,Zhou Wen-Di,Mu Xian-Min,Zhang Qian,Yu Chen,Di Bin,Su Meng-Xiang
Human 17β-hydroxysteroid dehydrogenase type 1 (17β-HSD1), a potential target in breast cancer prevention and therapy, was extracted from human placenta and immobilized on nonporous silica (∼5 μm) with a covalent method for the first time. The optimum initial enzyme concentration and immobilization time during the immobilization process were 0.42 mg mL −1 and 12 h, repectively. The binding was confirmed by scanning electron microscope (SEM) and infrared spectroscopy (FT-IR). It could improve the pH, thermal and storage stability compared to free enzyme. Moreover, the immobilized enzyme could be reused at least four times. A screening method based on it coupled with liquid chromatography–time-of-flight mass spectrometer (LC-TOF/MS) was established, and the half-maximal inhibitory concentration (IC 50) of apigenin for the immobilized enzyme was 291 nM. Subsequently, 10 natural products were evaluated leading to inhibition of the activity of 17β-HSD1 at the concentration of 25 μM, and six of them inhibit the activity over 50%.
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MICROCHIMICA ACTA
·
2016
·Vol. 184
·期 2
·547-555
·
IF 4.831
Zhang Lei,Gan Yangyang,Sun Haofan,Yu Bohao,Jin Xiaofeng,Zhang Runsheng,Zhang Weibing,Zhang Lingyi
Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were synthesized from resol, ZrO(NO 3 ) 2 , ferric acetylacetonate, and triblock copolymer F127. The method involves a multi-component co-assembly strategy associated with direct carbonization. The resulting carbon material is shown to be useful as a metal oxide affinity chromatography (MOAC) material for enrichment of phosphopeptides owing to its large mesoporous (4.8 nm) surface area (442 m 2 g −1 ), large pore volume (0.37 cm 3 g −1 ) and excellent hydrophilicity. The metallic iron and ferric oxide particles modified on the mesoporous carbon exert a magnetic force and, in combination with the metallic zirconia, is a viable MOAC material for enrichment of low-abundance phosphopeptides. Because of metal chelation between metallic nanoparticles and the phosphate groups of phosphopeptides, the zirconia/magnetic mesoporous carbon displays high selectivity even at a phosphopeptide/nonphosphopeptide molar ratio of 1:500. As little as 1.5 fmol of phosphopeptides become detectable. The MOAC was successfully applied to the identification by MALDI-TOF MS of phosphopeptides in human serum and nonfat milk.
Magnetic mesoporous carbon composites incorporating hydrophilic metallic nanoparticles were prepared and successfully applied to the enrichment of low-abundance phosphopeptides from human serum and nonfat milk by MALDI-TOF mass spectrometry.
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Food Analytical Methods
·
2016
·Vol. 10
·期 6
·1745-1754
·
IF 2.167
Wang Meng,Yang Fan,Liu Le,Cheng Chunsheng,Yang Yaling
A highly selective sample cleanup procedure combining ionic liquid-based surfactant extraction (ILSE) and magnetic dispersive μ-solid phase extraction (MD-μ-SPE) was triumphantly developed for the synchronously extraction of four phthalate acid esters (PAEs) in packaging milk samples prior to high-performance liquid chromatography coupled with photodiode array detector (HPLC-DAD). In this ionic liquid (IL)-based surfactant method, 1-octyl-3-methylimidazolium hexafluorophosphate ([C 8 MIM] [PF 6 ]) was used as extraction solvent, anionic surfactant sodium linear alkylbenzene sulfonate (LAS) was used as auxiliary extraction solvent, and then sodium chloride (NaCl) was mixed to drive phase separation. The synthesized hydrophobic diatomaceous earth-supported Fe 3 O 4 magnetic nanoparticles (DSMNPs) were applied as an efficient adsorbent to retrieve the analyte-containing IL and LAS. Under the optimal extraction situations, good linearity of the approach was obtained in the concentration range from 10 to 1000 ng/mL for target analytes, and the preconcentration process was rapidly accomplished in 5 min. The limits of detection (LODs) based on a signal-to-noise ratio (S/ N = 3) were ranged from 1.42 to 3.57 ng/mL with the relative standard deviations (RSDs) over the range of 1.84–3.56% ( n = 5). The above-mentioned method was applied to the trace analysis of four PAEs including benzyl butyl phthalate (BBP), dicyclohexyl phthalate (DCHP), di-n-butyl phthalate (DBP), and di-n-octyl phthalate (DNOP) in packaging milk samples, and recoveries were between 89.8 and 99.7%.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2016
·Vol. 53
·期 11
·4076-4083
·
IF 1.241
Chen Tao,Fang Sheng,Zuo Xiaobo,Liu Yanmei
The effects of microbial polysaccharides xanthan and curdlan on the pasting, viscoelastic properties and retrogradation of rice starch were studied. The additions of xanthan and curdlan increased the peak viscosities and decreased the final viscosities. The breakdown viscosities were increased by the curdlan addition, but were decreased by the xanthan addition. The dynamic viscoelastic results showed that the addition of xanthan in rice starch significantly increased storage modulus and loss modulus. The rice starch gel with xanthan addition exhibited higher resistances to the stress and produced a stronger microstructure network. The creep recovery data were well fitted by a 4-element Burger’s model. Differential scanning calorimetry showed that the addition of curdlan and xanthan decreased the melting enthalpy values and retarded the retrogradation of the rice starch gel during storage at 4 °C for 12 days. The pasting and thermal results demonstrated that the addition of xanthan could significantly inhibited the retrogradation of rice starch. It was concluded that the addition of curdlan and xanthan modified the rheology of rice starch gel in different ways and interacted under different models based on their molecular structures.
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MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 9
·2555-2562
·
IF 4.831
Wu Shijia,Li Qi,Duan Nuo,Ma Haile,Wang Zhouping
Microcystin-RR (MC-RR) is a highly acute hepatotoxin produced by cyanobacteria. It is harmful to both humans and the environment. A novel aptamer was identified by the systemic evolution of ligands by exponential enrichment (SELEX) method as a recognition element for determination of MC-RR in aquatic products. The graphene oxide (GO) SELEX strategy was adopted to generate aptamers with high affinity and specificity. Of the 50 aptamer candidates tested, sequence RR-33 was found to display high affinity and selectivity, with a dissociation constant of 45.7 ± 6.8 nM. Aptamer RR-33 therefore was used as the recognition element in a fluorometric assay that proceeds as follows: (1) Biotinylated aptamer RR-33 is immobilized on the streptavidinylated wells of a microtiterplate, and carboxyfluorescein (FAM) labelled complementary DNA is then allowed to hybridize. (2) After removal of excess (unbound) cDNA, sample containing MC-RR is added and incubated at 37 °C for 2 h. (3) Displaced free cDNA is washed away and fluorescence intensity measured at excitation/emission wavelengths of 490/515 nm. The calibration plot is linear in the 0.20 to 2.5 ng·mL −1 concentration range, and the limit of detection is 80 pg·mL −1 . The results indicate that the GO-SELEX technology is appropriate for the screening of aptamers against small-molecule toxins. The detection scheme was applied to the determination of MC-RR in (spiked) water, mussel and fish and gave recoveries between 91 and 98 %. The method compares favorably to a known ELISA. Conceivably, this kind of assay is applicable to other toxins for which appropriate aptamers are available.
The aptamer RR-33 specific for microcystin (MC-RR) was identified by using the graphene oxide (GO) SELEX process. This aptamer was used for determination of MC-RR by a fluorometric displacement assay.
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Nano Research
·
2016
·Vol. 9
·期 10
·2938-2949
·
IF 8.893
Li Le,Wang Kai,Huang Zhaoqi,Zhang Chao,Liu Tianxi
In this study, macroscopic graphene-wrapped melamine foams (MF-G) were fabricated by an MF-templated layer-by-layer (LBL) assembly using graphene oxide as building blocks, followed by solution-processed reduction. By concisely duplicating sponge-like, highly ordered three-dimensional architectures from MF, the resulting MF-G with an interconnected graphene-based scaffold and tunable nanostructure was explored as compressible, robust electrodes for efficient energy storage. A thin layer of pseudocapacitive polypyrrole (PPy) was then attached and uniformly coated on MF-G, resulting in a well-defined core–double-shell configuration of the MF-G-PPy ternary composite sponges. The as-assembled devices exhibited enhancement of supercapacitor performance, with a high specific capacitance of 427 F·g −1 under a compressive strain of 75% and an excellent cycling stability with only 18% degradation after 5,000 charge–discharge cycles. Besides, the MF-G-PPy electrode maintained stable capacitance up to 100 compression–release cycles, with a compressive strain of 75%. These encouraging results thus provide a new route towards the low-cost, easily scalable fabrication of lightweight and deformation-tolerant electrodes.
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MACROMOLECULAR RESEARCH
·
2016
·Vol. 24
·期 7
·602-608
·
IF 1.357
Shen Yueqin,Li Xian,Huang Yawen,Chang Guanjun,Cao Ke,Yang Junxiao,Zhang Renyi,Sheng Xueying,Ye Xu
pH and redox dual stimuli-responsive injectable hydrogels were prepared by cross-linking oxidized carboxymethyl cellulose (oxi-CMC) with 3,3′-dithiobis (propionohydrazide) (DTP) via Schiff base reaction under physiological condition. The hydrogels showed good performance such as tunable gelling time, appropriate rheology properties, high swelling ratio and low degradation rate. In vitro release studies confirmed that bovine serum albumin (BSA) as a model drug exhibited a sustainable release at pH 7.4 and an accelerated release under a lower pH 5.0 and/or reducing environments. The results signified that oxi-CMC/DTP hydrogels could be an attractive candidate for drug delivery system, tissue engineering or cell scaffold materials.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 22
·6115-6121
·
IF 3.125
Zhang Yun,Yang Hang,Yu Junping,Wei Hongping
In this study, a system of magnetic beads (MBs) coupled with catalytic fluorescent immunoassay for rapid and sensitive determination of HIV-1 capsid antigen p24 was developed. p24 was captured by antibody immobilized MBs, and the detection antibody was linked to horseradish peroxidase (HRP) through biotin–streptavidin recognition, catalyzing the oxidation of o -phenylenediamine (OPD) and hydrogen peroxide to produce a fluorescent product. This is the first reported utilization of the fluorescence of OPD oxidation product catalyzed by HRP for immunoassay. Optimization of conditions afforded a low detection limit of 0.5 pg/mL (3σ) for p24 with a linear range of 1.4–90.0 pg/mL. The assay exhibited good reproducibility with a relative standard deviation (RSD) of 4.4 %, 4.7 %, and 5.0 % for detecting 1.4 pg/mL, 22.5 pg/mL, and 45.0 pg/mL p24, respectively. The assay can be completed in less than 90 min. Moreover, the proposed method was successfully applied to detect p24 in spiked serum. This method overcomes the interference of MBs to the fluorescence signal and demonstrated higher sensitivity for detection of p24 than conventional ELISA kits. The system could be applied for detecting other antigens with high sensitivity, rapidity, specificity, and simple operation.
A rapid and sensitive biosensing method coupling immunomagnetic separation and catalytic fluorescence for determination of HIV-1 p24 has been developed
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 21
·5819-5827
·
IF 3.125
Zheng Qiqi,Yu Yonghua,Fan Kai,Ji Feng,Wu Jian,Ying Yibin
A nano-silver electrode immobilizing acetylcholinesterase (AChE) for the detection of organophosphorus (OPPs) pesticides is reported. Scanning electron microscopy (SEM) was used to characterize the surface structure of two kinds of electrodes fabricated with different sizes of silver powders and the interface between chitosan layer and nano-silver powder layer. Cyclic voltammetry was carried out to characterize the response of silver/chitosan electrode in the absence and in the presence of thiocholine (TCh). It was also used to evaluate the insulativity of the chitosan layer. An amperometric method was performed to measure the response of the electrode to TCh, which is the product of the enzymatic reaction for detecting organophosphorus pesticides indirectly. Although there are many kinds of nanoparticles, silver was chosen for its internal advantage in detecting TCh at low potential without further modification. The result shows nano-silver powder has better performance than usual silver powder, and the limit of detection of paraoxon is 4 ppb under optimized conditions. One percent (w/v) chitosan solution was used as binder for the immobilization of nano-silver powder and AChE, which made it possible for independent electrode fabrication at room temperature, whereas 3% (w/v) chitosan solution was used as insulating compound for controlling the electrode area. Unlike traditional organic insulating ink, chitosan is safe and environmentally friendly, and it is used as insulating material for the first time. The flexible nano-silver/AChE/chitosan electrode was evaluated in Chinese chives and cabbage , and the recoveries of standard addition were 105.11 and 96.41%, respectively. Owing to the antibacterial property of nano-silver and the biocompatibility, safety, and biodegradability of chitosan, the proposed method is safe, facile, environmentally friendly, and has great potential in organophosphorus pesticide detection for food safety.
Current response of nano-silver electrode ( a ) and silver electrode ( b ) to thiocholine in 0.02 M PBS + KCl at 0.15 V; addition of thiocholine (0.09 mM) every 50 s (↓); inset: calibration curve of nano-silver (▲) and silver (◆) electrode
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