ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2018
·Vol. 410
·期 8
·2211-2219
·
IF 3.307
Qu Fei,Mao Beibei,Xue Fangfang,Xia Lian,You Jinmao,Song Cuihua
In this paper, fluorescent Ag nanoclusters (Ag NCs) templated by hyperbranched polyethyleneimine (PEI) are utilized as a versatile probe through the photoinduced electron transfer (PET) between PEI-Ag NCs and G-quadruplex-hemin complexes. In the presence of hemin and target molecule, the specific conjugation with its aptamer induces the conformational change of the DNA sequence, releasing the G-quadruplex sequence part. Once the G-quadruplex-hemin complexes are introduced, electron transfer from the PEI-Ag NCs to G-quadruplex-hemin complexes occurs, resulting in fluorescence quenching. Through changing the sensing DNA sequence, this novel PET system enables the specific detection of target DNA and adenosine triphosphate (ATP) with the wide linear range of 1–200 nM and 5–500 nM, respectively, and the corresponding limit of detection as low as 0.3 nM for target DNA and 1.5 nM for ATP. In addition, the proposed method is successfully applied to the determination of ATP in human serum samples with satisfactory recoveries, and a logic gate is fabricated using target molecules and hemin as inputs and the fluorescence signal of PEI-Ag NCs as an output.
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 2
·1-8
·
IF 5.705
Cheng Xia,Cen Yao,Xu Guanhong,Wei Fangdi,Shi Menglan,Xu Xiaoman,Sohail Muhammad,Hu Qin
The authors describe a fluorometric aptamer based assay for adenosine triphosphate (ATP). It is based on the use of carbon dots (CDs) and graphene oxide (GO). The resultant CD-aptamer is adsorbed on the surface of GO via π-stacking and hydrophobic interaction, and the fluorescence of CD-aptamer is quenched via fluorescence resonance energy transfer (FRET) between CDs and GO. If ATP is present, it will bind to the aptamer and the CD-aptamer will be desorbed from GO. This will suppress FRET and the fluorescence of the CDs is restored. Under the optimal conditions and at typical excitation/emission wavelengths of 358/455 nm, the assay has a 80 pM detection limit and a linear range that extends from 0.10 to 5.0 nM concentrations of ATP. The method was successfully applied to the determination of ATP in yogurt samples. This method can also be conceivably applied to the detection of other analytes for which appropriate aptamers are available.
Schematic of a novel fluorometric ATP assay based on the fluorescence resonance energy transfer (FRET) between aptamer modified carbon dots (CD-aptamer) and graphene oxide (GO). CD-aptamer was used as the energy donor and molecular recognition probe, and GO acted as energy acceptor. This assay exhibits high sensitivity and selectivity with a detection limit as low as 80 pM.
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Scientific Reports
·
2018
·Vol. 8
·期 1
·1-9
·
IF 4.122
Yuan Dandan,Ding Lairong,Sun Zhaomei,Li Xuemei
Here, we report a novel magnetic resonance imaging (MRI)/fluorescence bimodal amplification platform for the detection of glutathione (GSH) on the basis of redoxable manganese dioxide (MnO 2 ) nanosheets, which can be readily applied as a DNA nanocarrier, fluorescence quencher, and intracellular GSH-activated MRI contrast agent. The binding of aptamers that absorbed on the MnO 2 nanosheets to their target can facilitating the endocytosis of target-nanoprobes. Once endocytosed, the MnO 2 nanosheets can react with cellular GSH, resulting in the disintegration of nanosheets to generate plenty of Mn 2+ ions for MRI and releases the primers which were adsorbed on the MnO 2 nanosheets. Then the rolling circle amplification (RCA) reaction was initiated to amplify the fluorescence signal. In addition, after treatment with GSH, the MnO 2 nanosheets were reduced and then most of the fluorescence was recovered. Therefore, this MnO 2 nanoprobe exhibits excellent selectivity, suggesting a potential detection platform for analyzing the glutathione level in cells.
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Scientific Reports
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2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Li Lili,Zhang Sheng,Xin Yanfei,Sun Junying,Xie Feng,Yang Lin,Chen Zhiqin,Chen Hao,Liu Fang,Xuan Yaoxian,You Zhenqiang
Quzhou Fructus Aurantii (QFA) is an authentic herb of local varieties in Zhejiang, China, which is usually used to treat gastrointestinal illnesses, but its effects on respiratory inflammation have not been reported yet. In our study, the anti-inflammatory activity of QFA extract (QFAE) was evaluated on copper sulfate pentahydrate (CuSO 4 ·5H 2 O)-induced transgenic neutrophil fluorescent zebrafish model. QFAE showed a significant effect of anti-inflammation in CuSO 4 ·5H 2 O-induced zebrafish by reducing the neutrophil number in the inflammatory site. We investigated the anti-inflammatory activity of QFAE on lipopolysaccharide (LPS)-induced acute lung injury (ALI) mice models and RAW 264.7 cells. QFAE had an anti-inflammatory effect on reducing total cells, neutrophils, and macrophages in BALF and attenuated alveolus collapse, neutrophils infiltration, lung W/D ratio, myeloperoxidase (MPO) protein expression and other pulmonary histological changes in lung tissues, as well as hematological changes. Levels of pro-inflammatory cytokines, including TNF, IL-6, IFN-γ, MCP-1, and IL-12p70, were decreased, whereas anti-inflammatory cytokine IL-10 was increased after treatment with QFAE both in vivo and in vitro . In summary, our results suggested that QFAE had apparent anti-inflammatory effects on CuSO 4 ·5H 2 O-induced zebrafish, LPS-induced ALI mice, and RAW 264.7 cells. Furthermore, QFAE may be a therapeutic drug to treat ALI/ARDS and other respiratory inflammations.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 10
·9636-9646
·
IF 2.8
Li Chenguang,Qu Ruijuan,Chen Jing,Zhang Shuo,Allam Ahmed A.,Ajarem Jamaan,Wang Zunyao
Triclosan (TCS) is an antibacterial and antifungal agent widely used in personal care products, and it has been frequently detected in the aquatic environment. In the present study, the acute toxicity of TCS to Daphnia magna , Photobacterium phosphoreum , Danio rerio , and Limnodrilus hoffmeisteri was assessed under different pH conditions. Generally, TCS was more toxic to the four aquatic organisms in acidic medium. The LC 50 values for D. magna and D. rerio were smaller among the selected species, suggesting that D. magna and D. rerio were more sensitive to TCS. In addition, the oxidative stress-inducing potential of TCS was evaluated in Carassius auratus at three pH values. Changes of superoxide dismutase (SOD) and catalase (CAT) activity, glutathione (GSH) level, and malondialdehyde (MDA) content were commonly observed in all TCS exposure groups, indicating the occurrence of oxidative stress in the liver of C. auratus . The integrated biomarker response (IBR) index revealed that a high concentration of TCS induced great oxidative stress in goldfish under acidic condition. This work supplements the presently available data on the toxicity data of TCS, which would provide some useful information for the environmental risk assessment of this compound.
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CELLULOSE
·
2018
·Vol. 25
·期 3
·1921-1938
·
IF 3.809
Peng Xiongyi,Su Siwei,Xia Minggui,Lou Kankan,Yang Feng,Peng Shuai,Cai Yingjie
Ramie fiber, one of the strongest and oldest natural fibers, has gained much interest in adsorption of heavy metal ions in water, however, it is severely limited due to the low response rates and low adsorption capability to heavy metal ions. A novel type of carboxymethyl-functionalized porous ramie microspheres (CPRM) were prepared with a combined emulsification-evaporation method and a carboxymethylation reaction, in order to effectively remove Cd(II) in water. XPS and FTIR characterizations confirmed that the carboxyl groups were introduced onto the CPRM. The diameters of the CPRM mainly lied within the range of 8–12 μm, and the BET surface area of the CPRM was 47.6 m 2 /g. SEM images confirmed a porous structure was present on the CPRM surface. The CPRM obtained a high adsorption capacity of Cd(II) (173.1 mg/g) at 303 K, which was higher than most reported conventional adsorbents. The adsorption isotherms and the kinetics were in agreement with the Langmuir isotherm model and the pseudo-second-order kinetic model. The process was both endothermic and spontaneous. The CPRM could also remove Cd(II), Pb(II), Ni(II), and Zn(II) from wastewater with various efficiencies. The Cd(II)-loading CPRM could be successfully regenerated by the HCl and NaOH treatment. The CPRM show good potential for purifying wastewater.
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Frontiers of Environmental Science & Engineering
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2018
·Vol. 12
·期 1
·1-8
·
IF 1.961
Gao Pan,Song Yuan,Wang Shaoning,Descorme Claude,Yang Shaoxia
The Fe 2 O 3 -CeO 2 -Bi 2 O 3 /γ-Al 2 O 3 catalyst, a novel environmental-friendly material, was used to investigate the catalytic wet air oxidation (CWAO) of cationic red GTL under mild operating conditions in a batch reactor. The catalyst was prepared by wet impregnation, and characterized by special surface area (BET measurement), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The Fe 2 O 3 -CeO 2 -Bi 2 O 3 /γ-Al 2 O 3 catalyst exhibited good catalytic activity and stability in the CWAO under atmosphere pressure. The effect of the reaction conditions (catalyst loading, degradation temperature, solution concentration and initial solution pH value) was studied. The result showed that the decolorization efficiency of cationic red GTL was improved with increasing the initial solution pH value and the degradation temperature. The apparent activation energy for the reaction was 79 kJ·mol –1 . Hydroperoxy radicals (HO 2 ·) and superoxide radicals (O 2 – ·) appeared as the main reactive species upon the CWAO of cationic red GTL.
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Food Analytical Methods
·
2018
·Vol. 12
·期 1
·271-281
·
IF 2.245
Chen Xue-Lei,Ai Lian-Feng,Cao Ya-Qing,Nian Qi-Xun,Jia Ye-Qing,Hao Yu-Lan,Wang Man-Man,Wang Xue-Sheng
A graphene oxide-incorporated monolithic column was fabricated and developed as an on-line solid-phase extraction sorbent coupled with LC-MS/MS for the automatic and fast determination of 16 sulfonamides in chicken muscle and milk samples. The proposed hybrid sorbent was easily produced from graphene oxide and a single crosslinker of ethylene glycol dimethacrylate. It gave favorable properties including uniformer structure, larger specific surface area, and enhanced column efficiency compared to the neat polymer monolith. Loading and eluting solutions affecting the on-line SPE were studied in detail. Under the optimized conditions, the on-line solid phase extraction coupled with LC-MS/MS method was achieved in 23 min. The developed method exhibited good linearity in the range of 1–100 μg/kg for sulfonamides in milk samples, and 2–200 μg/kg in chicken muscle samples, with r ranging from 0.993 to 0.999. The detection limits ( S / N = 3) were 0.3 μg/kg in milk and 0.6 μg/kg in chicken muscle. The spiked recoveries were in the range of 70.3–98.5% and 79.0–108.0%, with a precision of 5.19–9.37% and 4.04–8.57%, respectively. Furthermore, the graphene oxide-incorporated monolithic sorbent allowed more than 450 extraction cycles without any loss in the extraction efficiency. The developed method was successfully applied for the determination of 16 sulfonamides in milk and chicken muscle samples.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2018
·Vol. 29
·期 17
·15241-15248
·
IF 2.324
Ma Xiaochun,Liu Yanjun,Liu Han,Xu Bin,Zhou Jing,Xiao Fan
In this study, the microencapsulated phase change material with paraffin as core and inorganic TiO 2 shell was successfully synthesized by in situ hydrolysis and polycondensation of tetrabutyl titanate in a nonaqueous oil-in-water emulsion using formamide as aqueous phase. The chemical compositions, crystalline structure, morphology and microstructure of paraffin@TiO 2 microcapsules were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometer (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The thermal properties and thermal stability of the microcapsules were measured by differential scanning calorimeter (DSC) and thermo-gravimetric analyzer (TGA). FT-IR and XRD results proved that there was no chemical reaction between paraffin core and TiO 2 shell material. SEM images indicated that the paraffin@TiO 2 microcapsules showed a regular spherical shape with the average diameters of 2–5 µm with a smooth shell. SEM and TEM studies also confirmed the paraffin was fully encapsulated with TiO 2 . DSC analysis indicated that the microcapsules had similar phase change behaviors as those of pure paraffin. Typical sample froze at 52.24 °C with a latent heat of 110.42 J g −1 and melt at 60.41 °C with a latent heat of 107.54 J g −1 , of which the encapsulation ratio achieved to 54.58%. TGA results indicated the thermal stability of paraffin was improved by the protection of TiO 2 shell through the encapsulation.
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Scientific Reports
·
2018
·Vol. 8
·期 1
·1-11
·
IF 4.122
Sun Xia,Shi Junpeng,Fu Xiaoyan,Yang Yi,Zhang Hongwu
Near-infrared (NIR) persistent luminescence nanoparticles (NPLNPs) have become one of the most promising candidates for bioimaging. Different from the other fluorescence nanoprobes, the NIR persistent luminescence of NPLNPs can last for a long time after excitation, double exposure that is nanoparticles and light exist during the long-term bioimaging. However, to date, the potential risk of nanoparticles and NIR persistent luminescence of NPLNPs is still unknown. In this study, Cr3 + -doped zinc gallate, Zn 1.1 Ga 1.8 Sn 0.1 O 4 :Cr 3+ (ZGO), the most promising NPLNPs in bioimaging, was chosen as a representative for potential risk assessment. We evaluated the potential risk of nanoparticles and NIR persistent luminescence of ZGO for a long period of time. In vitro study showed that the ZGO possessed a low cytotoxicity. In vivo biodistribution results showed that the ZGO mainly accumulated in the reticuloendothelial system after intravenous injection and could be gradually cleared from the body by digestive system. In addition, the ZGO did not exhibit appreciable toxicity in mice over a period of 60 days. It’s also worth mentioning that long-term NIR persistent luminescence of ZGO did not exhibit obvious toxicities both in vitro and in vivo . Our results provide important information with regards to the risk of NPLNPs in long-term bioimaging.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2018
·Vol. 44
·期 11
·6621-6636
·
IF 1.674
Kang Chunli,Xiao Kunkun,Yao Zhifu,Wang Yuhan,Huang Dongmei,Zhu Ling,Liu Fang,Tian Tao
Pure phase ZnTiO 3 was prepared through a sol–gel process, then graphene-ZnTiO 3 nanocomposites were synthesized by a hydrothermal method using the prepared ZnTiO 3 nanoparticles and graphene oxide as precursors. X-ray diffraction results revealed the production of pure cubic ZnTiO 3 at 600 °C. ZnTiO 3 was anchored on the graphene nanosheets, demonstrating a spherical morphology in transmission electron microscope images. The existence of chemical bond Ti–O–C in the nanocomposites was proved by Fourier-transforming infrared spectroscopy. UV–Vis diffusive reflection spectra indicated that the absorption edge of the nanocomposites shifted towards the visible region. The photocatalytic activity of the composites was tested through the photocatalytic degradation of methyl blue under simulated solar irradiation. The results showed that the photocatalytic activity of the nanocomposites was obviously increased in contrast to pure ZnTiO 3 , which was strongly affected by the crystalline structure of ZnTiO 3 and the concentration of graphene. The enhanced photocatalytic activity was mainly attributed to the conglomeration inhibition of ZnTiO 3 nanoparticles, the electron transfer between ZnTiO 3 and graphene and the extended absorption range. Furthermore, other contaminants such as tetracycline, Rhodamine B and methyl orange were tested under the same conditions to investigate the photocatalytic performance of the photocatalysts. The reusability tests indicated that the prepared composites exhibited good stability.
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Scientific Reports
·
2018
·Vol. 8
·期 1
·1-9
·
IF 4.122
Xu Peiman,Li Jingwei,Luo Jiaxian,Wei Licheng,Zhang Dawei,Zhou Dan,Xu Weiming,Yuan Dingsheng
Earth-abundant and efficient bifunctional electrocatalysts for both hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) are highly significant for renewable energy systems. However, the performance of existing electrocatalysts is usually restricted by the low electroic conductivity and the limited amount of exposed active sites. In this work, (Fe 0.2 Ni 0.8 ) 0.96 S tubular spheres supported on Ni foam have been prepared by a sulfuration of FeNi layered double hydroxide spheres grown on Ni foam. Benefiting from the unique tubular sphere architecture, the rich inner defects and the enhanced electron interactions between Fe, Ni and S, this electrocatalyst shows low overpotential of 48 mV for HER at 10 mA cm −2 in 1.0 mol L −1 KOH solution, which is one of the lowest value of non-previous electrocatalyts for HER in alkaline electrolyte. Furthermore, assembled this versatile electrode as an alkaline electrolyzer for overall water splitting, a current density of 10 mA cm −2 is achieved at a low cell voltage of 1.56 V, and reach up to 30 mA cm −2 only at an operating cell voltage of 1.65 V.
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