Journal of Saudi Chemical Society
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2020
·
IF 3.517
Keliang Zhang,Jun Gao,Shangji Li,Ting Ma,Linhong Deng,Yong Kong
Mesoporous silica nanoparticles (MSN) have been widely used for drug delivery due to their large specific surface area and excellent biocompatibility. However, the mesoporous structure of MSN would lead to the inevitable “premature release” of the drugs, and therefore the modification of MSN for controlled delivery seems to be a necessary step. Herein, chitosan (CS) was used for the surface functionalization of MSN via amidation reaction, and the introduced CS could function as a “gatekeeper” and the drug of methotrexate (MTX) might be encapsulated in the mesopores of MSN. As a result, the “premature release” of the encapsulated MTX could be effectively circumvented with the aid of the CS cap. More importantly, the drug delivery from the hybrid of MSN and CS (MSN/CS) can be endowed with pH-sensitivity by the introduction of CS because the amide bonding between CS and MSN is highly pH-sensitive. The cumulative release of MTX from the MSN/CS is more pronounced at pH 5.0 (80.86%) than those at pH 6.8 (40.46%) and pH 7.4 (18.25%).
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高影响因子
Frontiers in Pharmacology
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2020
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IF 4.225
Li Zhoulei,He Peng,Luo Ganhua,Shi Xinchong,Yuan Gang,Zhang Bing,Seidl Christof,Gewies Andreas,Wang Yue,Zou Yuan,Long Yali,Yue Dianchao,Zhang Xiangsong
Background: The anticancer potential of pharmacologic ascorbic acid (AA) has been detected in a number of cancer cells. However, in vivo study suggested a strongly reduced cytotoxic activity of AA. It was known that pH could be a critical influencing factor for multiple anticancer treatments. In this study, we explored the influence of pH on the cytotoxicity of ascorbic acid. We employed castration-resistant prostate cancer (CRPC) cell lines PC3 and DU145 to observe the therapeutic effect of AA on PCa cells that were cultured with different pH in vitro. We also analyzed the influence of pH and extracellular oxidation on cytotoxicity of AA in cancer cells using reactive oxygen species (ROS) assay, cellular uptake of AA, and NADPH assay. Male BALB/c nude mice bearing prostate carcinoma xenografts (PC3 or DU145) were used to assess treatment response to AA with or without bicarbonate in vivo. The cellular uptake of AA in PCa xenografts was detected using positron emission tomography (PET). Small animal PET/CT scans were performed on mice after the administration of 6-deoxy-6-[18F] fluoro-L-ascorbic acid (18F-DFA).Results: Our in vitro studies demonstrate that acidic pH attenuates the cytotoxic activity of pharmacologic ascorbic acid by inhibiting AA uptake in PCa cells. Additionally, we found that the cancer cell-selective toxicity of AA depends on ROS. In vivo, combination of AA and bicarbonate could provide a significant better therapeutic outcome in comparison with controls or AA single treated mice. 18F-DFA PET imaging illustrated that the treatment with NaHCO3 could significantly increase the AA uptake in tumor.Conclusions: The alkalinity of tumor microenvironment plays an important role in anticancer efficiency of AA in CRPC. 18F-DFA PET/CT imaging could predict the therapeutic response of PCa animal model through illustration of tumoral uptake of AA. 18F-DFA might be a potential PET tracer in clinical diagnosis and treatment for CRPC.
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PubMed
高影响因子
Nanomaterials
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2020
·Vol. 10
·期 10
·2000
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IF 4.324
Yongpan Shan,Chunli Xu,Hongjun Zhang,Huiping Chen,Muhammad Bilal,Shujun Niu,Lidong Cao,Qiliang Huang
Recently, metal–organic frameworks (MOFs) have become a dazzling star among porous materials used in many fields. Considering their intriguing features, MOFs have great prospects for application in the field of sustainable agriculture, especially as versatile pesticide-delivery vehicles. However, the study of MOF-based platforms for controlled pesticide release has just begun. Controlled pesticide release responsive to environmental stimuli is highly desirable for decreased agrochemical input, improved control efficacy and diminished adverse effects. In this work, simple, octahedral, iron-based MOFs (NH2-Fe-MIL-101) were synthesized through a microwave-assisted solvothermal method using Fe3+as the node and 2-aminoterephthalic acid as the organic ligand. Diniconazole (Dini), as a model fungicide, was loaded into NH2-Fe-MIL-101 to afford Dini@NH2-Fe-MIL-101 with a satisfactory loading content of 28.1%. The subsequent polydopamine (PDA) modification could endow Dini with pH-sensitive release patterns. The release of Dini from PDA@Dini@NH2-Fe-MIL-101 was much faster in an acidic medium compared to that in neutral and basic media. Moreover, Dini@NH2-Fe-MIL-101 and PDA@Dini@NH2-Fe-MIL-101 displayed good bioactivities against the pathogenic fungus causing wheat head scab (Fusarium graminearum). This research sought to reveal the feasibility of versatile MOFs as a pesticide-delivery platform in sustainable crop protection.Keywords:metal–organic framework;polydopamine;pesticide;pH-sensitive release;fungicidal activity
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高影响因子
Small
·
2020
·Vol. 16
·期 42
·2003429
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IF 11.459
Yingnan Liu,Yaqing Xiao,Min Yu,Yuanyuan Cao,Yalan Zhang,Taotao Zhe,Hui Zhang,Li Wang
Antimonene quantum dots (AMQDs) are attracting considerable attention due to their fascinating physicochemical properties. However, research on their semiconductor characteristics, especially the photoluminescence performance, is still in a preliminary stage and the experimental verification is scarcely reported, significantly restricting their further applications. Herein, the photoluminescence property of AMQDs is experimentally verified. The AMQDs are prepared by probe sonication-assisted liquid-phase exfoliation and show robust blue fluorescence, and the photoluminescence is hardly affected by pH. In view of the derivatization reaction of tetracyclines (TET) at different pHs, AMQDs are developed as a pH-mediated dual-channel ratiometric fluorescent probe for TET detection. Under acidic conditions, the AMQDs’ probe exhibits unique recognition behavior due to the inherent fluorescence of TET and the solvent-enhancing effect, that is, the fluorescence changes from blue to red. Under alkaline conditions, this fluorescent probe realizes the transition from blue to yellow-green because of the decomposition of TET. The limits of detection are 27 × 10 −9 and 74 × 10 −9 m , respectively. The high sensitivity and remarkable fluorescence changes make AMQDs ideal probes for TET sensing. Additionally, this is the first report on the photoluminescence property of AMQDs. It is believed that this work will open a new avenue for AMQDs in optical sensing fields.
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高影响因子
ACS Omega
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2021
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IF 3.512
Shuhua Chang,Dawei Qin,Rongjun Yan,Mengli Zhang,Binglin Sui,Hui Xu,Zhaomin Zheng,Xiaoli Hou,Yonggang Wang,Chenggang Qi
pH- and temperature-sensitive nanogels (NGs) were prepared from sodium alginate (SA) and N-isopropylacrylamide (NIPAM), as the sensitivity at pH 5.5 and 31 °C. SA was pH-modified with glutamic acid (Glu) and ethylenediamine (EDA). The products Glu-SA (Glu-modified SA) and EGSA (EDA- and Glu-modified SA) were characterized by ninhydrin color reaction, infrared spectroscopy, and zeta potential, and the best reactant ratio was selected. Moreover, temperature-sensitive, pH-sensitive EGSA-NGs possessing a semi-interpenetrating network structure were prepared by radical polymerization using N-isopropylacrylamide. The morphology of EGSA-NGs was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM). The cytotoxicity test shows the low cytotoxicity and high biocompatibility of the NGs. The newly prepared NGs were also subjected to pH-sensitive temperature-sensitive in vitro drug-loading and drug-release experiments. The pH-sensitive and temperature-sensitive experiments showed that the particle size of EGSA-NGs was reduced at pH 5.5 and above 31 °C. The drug-loading and drug-release experiments also confirmed this finding, indicating that the newly synthesized NGs could release the drug according to the environmental changes. Therefore, the material has potential application value in solid tumor targeted therapy.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Ziming Yang,Puwang Li,Yu Chen,Qiang Gan,Zhipan Feng,Yiguang Jin,Chuang Zhou,Zuyu He,Chao Wang,Yunhao Liu,Changgen Feng
A novel pH/glutathione (GSH) multi-responsive chitosan nanoparticles (NPs) material has been successfully designed and prepared by a self-assembly/self-crosslinking method for photodynamic therapy (PDT), which overcomes the shortcomings of traditional photosensitizer carriers, such as poor chemical stability, low loading efficiency and single-responsive photosensitizer release. Amphiphilic sulfhydryl chitosan (SA-CS-NAC) is first prepared by modifying chitosan (CS) with stearic acid (SA) and N -acetyl-L-cysteine (NAC), and then subject to self-assembly and self-crosslinking in the presence of photosensitizer, indocyanine green (ICG), to form the ICG-loaded amphiphilic sulfhydryl chitosan nanoparticles (SA-CS-NAC@ICG NPs). The ICG entrapment efficiency and loading efficiency of the NPs are found to be 95.2% and 27.6%, respectively. The multi-responsive ICG release of the NPs to the low pH and high GSH content of the microenvironment in tumor cells is successfully achieved. Under the laser irradiation, the SA-CS-NAC@ICG NPs produce the amount of reactive oxygen species (ROS) twice of that generated by free ICG under the same conditions. The in vitro cell experiment confirmed the strong cellular uptake ability, low biotoxicity and good tumor inhibition of the NPs. Our work has provided a new strategy for the targeted photosensitizer delivery for PDT.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
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2020
·Vol. 55
·期 35
·16718-16729
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IF 3.553
Wang Chen,Wen Hao,Guo Hailing,Wang Xueyan,Shen Yuhua,Xie Anjian
A novel ferroferric oxide (Fe 3 O 4 )@void@zinc oxide (ZnO) nanocomposite was constructed through a template-assisted selective etching method. The experiment results showed that the magnetic saturation value of nanocomposite could reach to 44.07 emu g −1 , indicating the favorable magnetic targeting function. The presence of the void endowed the nanocomposite with high drug doxorubicin (DOX) loading capacity (85.65 μg mg −1 ). The pH-sensitive drug release was realized due to the gating effect of ZnO, and the DOX release rates were 92.7%, 60.8% and 23.4% at the pH values of 5.0, 6.5 and 7.4, respectively (37 °C). Besides, the Fe 3 O 4 @void@ZnO-DOX exhibited desirable antitumor effects attributed to their cellular uptake and above advantages. Therefore, the Fe 3 O 4 @void@ZnO nanocomposite may serve as a promising drug carrier in cancer therapy and other biomedical applications.
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高影响因子
ACS Applied Materials & Interfaces
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2020
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IF 8.758
Weili Xue,Ashish Trital,Jian Shen,Longgang Wang,Shengfu Chen
To augment the antitumor efficacy and minimize the significant side effects of chemotherapeutic drugs on health organs, a novel albumin-mimicking nanodrug, which is based on zwitterionic poly(glutamatyl lysine-co-cysteine) peptides scaffold, is developed to enhance pH-triggered tumor targeting via prolonging circulation time and accelerating cellular internalization. Results showed that the internalization of the nanodrug by MCF-7 cells is much faster than that by Doxil and even comparable to that by free doxorubicin (Dox) at tumor microenvironmental pH 6.7, whereas the internalization of the nanodrug is only 27.4 ± 7.6% of the Doxil by RAW-264.7 cells. Moreover, the significantly prolonged circulation time of the “stealthy” nanodrug was also comparable to that of the long circulating Doxil. As a result, the accumulation of the nanodrug in the tumor is much higher than that in the liver and kidney before the circulation half-life, which is significantly different from most other nanodrugs accumulated in the liver and kidney in this time scale. The tumor inhibition rate of the nanodrug was much higher than that of Doxil (93.2 ± 3.0% vs 54.2 ± 6.5%) after 18 day treatment, while the average bodyweight of the mice treated by the nanodrug was 26.9 ± 6.7% higher than that by Doxil. This indicated that the synergetic effect of long circulation time and fast cellular internalization of the nanodrug can significantly augment tumor targeting. This method might rejuvenate the traditional chemotherapeutic treatment.
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PROCESS BIOCHEMISTRY
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2020
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IF 2.952
Yan Zhang,Hongzhou Zhang,Dong He,Xuejun Cao,Junfen Wan
Aqueous two-phase systems have been widely studied for the separation and recovery of bioproducts. In this study, the thermosensitive polymer EO 30 PO 70 and pH-responsive polymers (P ADB4.66 and P ADB6.26 ) were used to develop a recyclable aqueous two-phase system suitable for spiramycin partitioning. EO 30 PO 70 represents poly-(ethylene oxide-propylene oxide), where 30 and 70 indicate the proportions of ethylene oxide and propylene oxide, respectively. P ADB4.66 and P ADB6.26 represent poly-(acrylic acid-dimethylamine ethyl methacrylate-butyl methacrylate), where the numbers (4.66 and 6.26) in the subscripts indicate the isoelectric point of the polymers. The phase separation mechanism of ATPS was studied through low-field nuclear magnetic resonance (LF-NMR) and surface tension. Moreover, the parameters affecting spiramycin partitioning, such as system components and composition, pH, temperature, and type and concentration of additive salt, were investigated. The best partitioning conditions were determined using 4.70% P ADB6.26 and 31.67% EO 30 PO 70 in the presence of 0.05 mol/L NaSCN at 35 °C and pH = 9.2. The highest partition coefficient of spiramycin from fermentation broth was 11.92, with an extraction recovery of 97.69%. In addition, phase-forming polymers P ADB6.26 and EO 30 PO 70 could be recovered by regulating the pH or temperature, and their recoveries reached 92.39% and 98.76%, respectively.
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高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2020
·
IF 5.065
Wei Long,Hui Ouyang,Chaoqun Zhou,Weimin Wan,Shengxian Yu,Kai Qian,Meiying Liu,Xiaoyong Zhang,Yulin Feng,Yen Wei
Molybdenum disulfide (MoS 2 ) is one of the most promising two-dimensional (2D) materials after graphene oxide . Its excellent properties make it promising for various applications, especially in the field of biomedicine owing to its small size, unique 2D morphology and photothermal conversion capacity. However, the lack of specific surface functional groups has still largely impeded their surface modification and for drug loading and controlled release. In this paper, the surface of MoS 2 sheets was modified by hydrazine groups, which could form dynamic hydrazone bonds with CHO-PEG and doxorubicin hydrochloride (DOX) to construct the PEGylated MoS 2 based drug delivery systems. The structure, thermal stability, surface morphology and particle size of MoS 2 related materials were characterized by different equipment. The characterization results confirm that these functionalized MoS 2 (MS-CO-NH) composites have been successfully prepared through the formation of dynamic bonds. On the other hand, the release of MS-P-D composites loaded with CHO-PEG and DOX showed that pH responsive behavior, and had a good sustained-release effect. More importantly, cell viability and internalization results showed that MS-CO-NH material had excellent biocompatibility. MS-P-D composite has good cancer treatment effect, and can transport DOX into HepG2 cells and slowly release DOX into the nucleus to kill cancer cells. In view of the results, we believe that this new MoS 2 -based vector is expected to be a candidate for controlling intracellular drug delivery and cancer treatment.
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高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2020
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IF 2.69
Penghui Wang,Wei Liu,Shuai Liu,Rong Yang,Yajie Pu,Wenjie Zhang,Xiaoxue Wang,Xin Liu,Yanhan Ren,Bo Chi
Here, a novel pH-responsive block copolymer, poly (ethylene glycol)-poly(ε-caprolactone)-poly(L-histidine) (PEG-PCL-PHis), was synthesized and designed for anti-cancer drug delivery with excellent biocompatible, biodegradable, and strong drug loading efficiency. 1H-NMR, IF-IR, and GPC were used to characterize the structure of the PEG-PCL-PHis copolymer. In addition, the morphology, particle size, Zeta potential, and critical micelle concentration (CMC) of different degree of polymerization were determined by transmission electron microscopy (TEM), dynamic light scattering granulometer (DLS), and fluorescence spectrometer, respectively. The strong affinity between the core of micelles and hydrophobic drug was manifested with 15.09% drug loading content and 84.65% entrapment efficiency. In vitro release of DOX from the block copolymer micelle demonstrated, the PEG-PCL-PHis copolymer micelle has stable and durable drug releasing ability accompanied with pH-sensitivity. From the mechanism of cellular uptake the micelles, the pathway of drug release was captured by confocal laser scanning microscope. These experiments demonstrated the safe delivery for anticancer medicine through this novel copolymer. In conclusion, the PEG-PCL-PHis copolymer micelle has great potential to become a safe drug carrier for cancer chemotherapy.
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PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
Xuejun Ruan,Xin Ren,Wenting Zhou,Qi Cheng,Zheng Yao,Wenqi Yu,Lujiang Jin,Liyi Shi
This article systematically studies the relationship between the dispersity of the mixed suspension, the microstructures and the electrical properties of the ZnO varistors by adjusting the dispersant content and the pH value. The suspension dispersity was measured by viscosity, zeta potential , particle size distribution and TEM observation. The structure of the as-prepared ZnO varistors was characterized by XRD and SEM. The electrical properties were investigated by testing E-J characteristics, breakdown voltage, leakage current, nonlinear coefficient, 8/20 μs waveform current impulse and accelerated aging performance. When the suspension reached the best dispersion condition of 0.3 wt% dispersant and the original pH value, the ZnO varistors had optimum electrical properties: breakdown voltage gradient of 299.95 V mm −1 , leakage current I L of 0.33 μA, voltage ratio U 5kA /U 1mA of 1.686, nonlinear coefficient of 46.71, and a downward trend of power consumption in the accelerated aging test.
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高影响因子
危险品化学品经营许可证（带存储）