EMBO REPORTS
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2026
·Vol. 27
·期 12
·3303-3339
·
IF 6
Guo Ruiyang,Guo Yabo,Shu Runguo,Qian Jiajia,Wang Jiawei,Li Ruobing,Qin Ti,Wang Ziyi,Tian Hongtao,Wu Mengchen,Zhou Long,Guo Xiaogang,Zhang Shan
Eukaryotic mRNAs typically encode a single functional polypeptide, a principle challenged by the discovery of widespread non-canonical peptide-coding ORFs within 5’UTRs. However, their functional significance at the protein level remains underexplored. Using a four-layered pipeline, we identify 14 human transcripts predominantly transcribed in polycistronic forms, each encoding two conserved proteins. Focusing on the SLC35A4 transcript, we show that its 5’UTR encodes a mitochondrial inner membrane-localized microprotein that we name STREMI ( S LC35A4 s t ress re sponse regulating M ICOS i nteractor). Sharing topology and motifs with the MICOS core subunit MIC10, STREMI regulates mitochondrial cristae morphogenesis in mice and human cells. Additionally, the STREMI-encoding uORF mediates stress-responsive translation of SLC35A4 —a Golgi nucleotide sugar transporter—upregulating its translation during the integrated stress response. Evolutionary analyses indicate that these bicistronic transcripts likely arose through transcriptional readthrough following retroposition. We propose a mechanism of “gene symbiosis” that enables functional partitioning and coordinated translation of protein pairs from bicistronic transcripts.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2026
·Vol. 132
·期 8
·710
·
IF 3
Guo Lin,Shi Zhitian,Zhan Jifan,Qu Lanfeng,Tan Jiawei,Yan Tingting
Large-scale bone defects resulting from trauma, infection, aging, tumors, and other diseases present significant clinical challenges. Current treatment modalities primarily involve surgical intervention, chemotherapy, and radiotherapy. However, conventional bone repair materials exhibit limitations in addressing tumor-associated defects, including inadequate drug loading capacity, uncontrolled release kinetics, and insufficient mechanical adaptability. To address these deficiencies in existing bone repair materials, we developed a novel composite bone scaffold utilizing a biphasic calcium phosphate (BCP) osteoconductive matrix. This matrix was integrated with polyacrylamide-modified gelatin (GelMA) hydrogel to enhance mechanical strength and oxidized sodium alginate (OSA) hydrogel containing pH-sensitive acylhydrazone bonds. The incorporation of the hydrogels significantly increased the compressive strength of the composite scaffold from 1.920 MPa to 2.515 MPa. Furthermore, the scaffold was physically adsorbed with the anti-tumor drug bortezomib (BTZ), demonstrating sustained release under simulated physiological conditions (pH 7.4). Notably, the release rate accelerated under acidic conditions that mimic the tumor microenvironment (pH 5.4 and 6.8).
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Nature Communications
·
2026
·
IF 18.1
Li Qiaodong,Yao Jingming,Wang Yiran,Liu Xinyu,Lei Jiuwei,Yin Wen,Yu Zhixuan,Wang Shaojie,Li Lin,Yan Xinlin,Shao Zongpu,Wang Zhenyu,Xia Wei,Chen Yue,Yu Chuang,Wang Lin,Wen Bin,Xu Bo,Huang Jianyu,Zhang Long
The development of high-energy all-solid-state batteries is critically hindered by the electrochemical instability of solid electrolytes against high-voltage oxide positive electrodes. While fluorination is a promising strategy to enhance electrolyte stability, conventional methods are ineffective, resulting in insufficient fluorine content and a debilitating trade-off with ionic conductivity. Here, we report a solid-state anion-exchange strategy that overcomes these limitations by producing core-shell Li-fluoride/LiCl nanocomposite precursors. These precursors enable the synthesis of heavily fluorinated lithium-halide and lithium-sulfide electrolytes that combine high ionic conductivity with good oxidative stability. This stability originates from the formation of a robust, self-limiting LiF-rich interphase at the positive electrode. Consequently, an all-solid-state battery using a Li-rich oxide positive electrode achieves high performance, retaining over 77.5% capacity after 2000 cycles at a high rate of 3 C (1 C = 275 mA/g) and a 5.0 V cutoff. This anion-exchange approach is broadly applicable to other systems and establishes a versatile platform for designing advanced fluorinated materials for next-generation batteries.
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JOURNAL OF FLUORESCENCE
·
2025
·Vol. 36
·期 2
·1445-1456
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IF 3.1
Huang Weihui,Tang Jidong,Ma Wenjin,Tao Xiaoqi
Structure-switching aptamers (SSAs) generate measurable signals through target-induced conformational changes, serving as sensitive molecular recognition elements. We developed a dual-fluorescence SSA for ochratoxin A (OTA) detection by inserting 2-aminopurine (2AP) into the OTA aptamer and hybridizing a complementary DNA (cDNA) strand at the 5’ end. OTA binding induced the aptamer-cDNA duplex to form a G4, enhancing 2AP fluorescence at 370 nm. Together with the intrinsic fluorescence of OTA at 450 nm, this enabled ratiometric dual-fluorescence measurements without external quenchers or fluorophores. To elucidate the molecular mechanism, all-atom molecular dynamics (MD) simulations and spectroscopic experiments assessed the effects of cDNA hybridization sites and strand lengths on sensing performance. Simulations further examined OTA binding at various 2AP insertion sites. Apt@2AP-cDNA1, with cDNA hybridized to bases 1–12, exhibited a binding pattern closely resembling the original aptamer. Umbrella sampling simulations revealed similar binding affinities: 46.3 kJ/mol for Apt@2AP-cDNA1 and 43.2 kJ/mol for the original aptamer. Experimentally, Apt@2AP-cDNA1 converted to G-quadruplexes more efficiently than other designs. A ratiometric dual-fluorescence aptasensor using Apt@2AP-cDNA1 was developed, with a linear detection range of 10–800 nM, a detection limit of 5.9 nM. The dual-fluorescence ratiometric aptasensor has good selectivity and was proven effective for detecting OTA in beer samples.
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Communications Chemistry
·
2026
·
IF 6.9
Fu Jiayi,Jiang ChengRui,Xiang Wanping,Chang Guangtao,Li Ruoxin
Flexible hydrogel-based strain sensors have attracted increasing attention for applications in wearable electronics and health monitoring due to their tissue-like mechanical properties and electrochemical responsiveness. However, most existing hydrogels suffer from mechanical brittleness, environmental instability, and humidity-sensitive conductivity, which limit their use in dynamic human motion detection. This study presents a high-performance flexible strain sensor based on PALCC hydrogel, which integrates hydrophobic association, ionic coordination, and deep eutectic solvent (DES) to enhance both mechanical and electrical properties. The hydrogel demonstrates exceptional mechanical strength (296.4 kPa), high elongation (434.6%), and stable electrical conductivity, making it ideal for wearable electronics and health monitoring applications. The synergistic interactions within the hydrogel network, including covalent bonding, ionic crosslinking, and hydrophobic associations, enable a two-stage linear strain response with outstanding durability (over 2000 cycles). Molecular dynamics simulations further elucidate the dynamic co-aggregation and strengthening mechanisms of the network. The sensor shows excellent dual-mode strain sensing under both tensile and compressive deformations, stable signal output for human motion and vocal vibration detection, and the capability to transmit information through Morse code via fingertip pressure. These results provide a promising solution for real-time health monitoring and wearable sensor applications, paving the way for future advancements in flexible strain-sensing technologies.
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Nature Communications
·
2026
·
IF 18.1
Yang Jiawei,Zhu Rui,Li Ming,Mei Zengxia,Chen Ling,Wu Li-Ming
Sustainable thermal management of high-heat-flux electronics beyond Moore’s Law requires micro thermoelectric coolers (μ-TECs) that are both high-performance and environmentally sustainable. However, state-of-the-art μ-TECs rely on Bi 2 Te 3 -based materials, whose tellurium scarcity, toxicity concerns and limited mechanical robustness hinder widespread adoption. Mg-based thermoelectric materials offer a promising alternative but remain challenging to integrate into microscale devices. Here we show that a low-temperature, water-free fabrication strategy enables tellurium-minimized μ-TECs based on n -type Mg 3 (Bi,Sb) 2 and p -type MgAgSb. Using magnetron sputtering as a cold bonding approach, we fabricate compact 12-pair microdevices (2.95 × 4.35 × 1.4 mm 3 ), with thermoelectric legs approximately 3% the size of previously reported Mg-based devices. The resulting μ-TECs achieve a power density of 4.34 W cm −2 and a packing density of 93.5 pairs cm −2 . Our work establishes a scalable route for sustainable thermoelectric cooling and a viable alternative to conventional Bi 2 Te 3 technologies.
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Scientific Reports
·
2026
·
IF 4.9
Pan Hong,Wang Qi,Ren Yingge,Ma Dongfang
The widespread application of neonicotinoid pesticides (NEOs) has caused severe contamination of aquatic environments, posing serious threats to water resources and ecological sustainability. In this study, graphitic micro-mesoporous composite carbon (GMC) was synthesized via template-assisted method and employed for the adsorption of NEOs from water. Structural characterization confirmed that GMC possessed graphitized framework, well-developed micro-mesoporous composite architecture, and abundant functional groups, which enhanced its adsorption performance. The GMC exhibits excellent adsorption performance for NEOs, with adsorption equilibrium achieved within 120 min. Batch adsorption experiments showed that the adsorption kinetics were consistent with both pseudo-first-order and pseudo-second-order models, while the adsorption isotherms followed the Freundlich model. This indicates that the adsorption process is spontaneous, endothermic, and tends toward physical adsorption. The maximum adsorption capacity for Thiamethoxam (THM) was achieved 1421.3 mg/g at pH = 3, whereas that for Nitenpyram (NIM) was obtained 642.7 mg/g at pH = 7, suggesting that the ionization degree of NEOs significantly affects the adsorption performance of GMCs. Five consecutive adsorption–desorption cycles validated the excellent stability and reusability of GMC. Co-existing ions and humic acid (HA) exerted negligible effects on adsorption performance. Furthermore, the GMC exhibits good adsorption performance in multi-pesticide coexistence systems and actual farmland surface water, demonstrating the adaptability of GMC to complex water matrices. This study reveals that the adsorption process of NEOs on GMCs is better described by interactions dominated by physical adsorption, driven by a variety of physical mechanisms, including strong π–π stacking, hydrogen bonding, and hydrophobic interactions. The excellent stability, sustainability, and reliability of GMC indicate its considerable potential for practical applications in water purification and environmental remediation.
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NATURE MATERIALS
·
2026
·
IF 38
Jin Hongrun,Zhou Wanhai,Li Jinchi,Li Xinran,Yang Zefang,Wang Chongran,Zhang Yanyan,Wu Ningyu,Feng Yutong,Li Gaoyang,Sun Zhihao,Yu Xiaoyu,Zhang Junwei,Yang Zhuo,Zhang Tengsheng,Ding Shixiang,Song Xinxin,Liu Lin,Wang Yifeng,Ye Chao,Li Laiquan,Li Wei,Liu Xin,Zhao Dongyuan,Fan Hong Jin,Chao Dongliang
Conventional vanadium chemistries have been hindered by limited capacity inherent to the ion-insertion mechanism and modest redox potentials in near-neutral media, constraining their suitability for high-energy aqueous batteries. Here we demonstrate a conversion-type vanadium redox that delivers concomitant superiority in capacity and potential for aqueous batteries. OH − -mediated chemical activation promotes V–O bond cleavage, thereby driving a transition from the single-electron insertion reaction to the four-electron conversion reaction. As confirmed by in situ synchrotron characterizations, a tailored mesoporous architecture enriches local OH − , facilitating the reversible conversion between V 2 O 3 and Na 3 VO 4 . The conversion anode delivers a high specific capacity of 700 mAh g −1 with 98% vanadium utilization, a low redox potential of −0.95 V versus standard hydrogen electrode and reversible cycling over 3,000 cycles. An alkaline Ni–V battery is fabricated, with a projected whole-battery-level energy density of up to 110 Wh kg −1 . This work paves the way to tuning the reaction pathway and offers insights into multielectron redox design for next-generation aqueous batteries.
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JOURNAL OF SOILS AND SEDIMENTS
·
2026
·Vol. 26
·期 7
·254
·
IF 3.3
Hu Linchao,Jiang Xianlu,Fan Dongyuan,Zhang Wenyi,Mao Linqiang
Purpose To address the key challenges of inefficient desorption of hydrophobic organic pollutants and limited efficiency of electrokinetic remediation in polycyclic aromatic hydrocarbons (PAHs) contaminated soils, this study investigates an enhanced electrokinetic remediation technique involving the sequential combination of surfactants and dual oxidants. The study examines its removal efficiency for the typical PAHs contaminant, phenanthrene, and its synergistic mechanism, with the goal of providing a theoretical basis for the efficient remediation of low-permeability organic-contaminated soils. Methods Using artificially prepared aged phenanthrene-contaminated soil as the study subject, four experimental groups were designed. We employed a sequential combination strategy of “solubilization followed by oxidation” was employed. The dynamic changes in current were monitored, electroosmotic flow, soil pH, and the migration and distribution of persulfate, combined with electron paramagnetic resonance (EPR) radical identification and high-performance liquid chromatography (HPLC) quantitative analysis, and gas chromatography–mass spectrometry (GC-MS) to analyze intermediate products, The relationship between surfactants and dual oxidants as well as their influence on the degradation pathways of PAHs. Results Compared with electrokinetic remediation alone, the average removal efficiency of phenanthrene using electrokinetic remediation alone was only 5.02%; which increased to 22.8% after the addition of sodium persulfate. Further introduction of Tween 80 increased the average removal efficiency to 54.44%, while the addition of hydrogen peroxide resulted in an average removal efficiency of 63.9%, with a removal efficiency reaching to 85.3% near the anode. Electron paramagnetic resonance (EPR) spectroscopy detected the simultaneous presence of sulfate radicals (SO₄·⁻) and hydroxyl radicals (·OH) in the reaction system, with the latter predominating; the addition of hydrogen peroxide significantly enhanced the radical signal intensity. The migration distance of persulfate increased after the addition of Tween 80, but the introduction of hydrogen peroxide accelerated the consumption of persulfate. Gas chromatography–mass spectrometry (GC-MS) analysis of the degradation intermediates of phenanthrene suggested a potential degradation pathway. Conclusion The sequential ‘solubilization-first, oxidation-second’ electrokinetic remediation strategy proposed in this study effectively promotes the desorption and migration of phenanthrene from soil and significantly improves phenanthrene removal efficiency through the synergistic action of surfactants and dual oxidants. Radical oxidation is the primary mechanism for phenanthrene degradation, with hydroxyl radicals playing a dominant role. Analysis of degradation products detected intermediates such as 9,10-phenanthrenequinone and 9-fluorenone. This study provides important theoretical support for understanding the mechanism and engineering applications of the surfactant-oxidant combined electrokinetic remediation technology.
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Biomarker Research
·
2026
·
IF 14.6
Ruixin Wu,Licheng Zhang,Peter JihCheng Wong,Chunming Sun,Yuan Feng,Xiaoyu Wang,Minjie Fu,Jinsen Zhang,Fufang Qiu,Xi Chen,Zhen Fan,Minfeng Shu,Wei Hua
IDH mutant gliomas, driven by the oncometabolite 2-hydroxyglutarate (2-HG), are associated with profound neurological morbidity and premature mortality. To address the unmet therapeutic needs, we investigated the mechanistic interplay between MTHFD2-driven one-carbon metabolism and ferroptosis susceptibility in these tumors. Our findings revealed that MTHFD2 upregulation, mediated through loss of m5C modification in chromatin associated RNAs (caRNAs)-establishes a metabolic vulnerability to ferroptosis. Crucially, we identified TAF15 as a pivotal RNA-binding protein that orchestrates the spatial recruitment of TET2 by bridging NSUN5-mediated RNA m5C methylation. Therapeutically, combinatorial targeting of MTHFD2 with its selective inhibitor and the hypomethylating agent decitabine induced ferroptosis in patient-derived IDH-mut glioma organoids, demonstrating potent ferroptosis activation. This work delineates an RNA epitranscriptomic-metabolism axis in glioma pathogenesis and provides a translational roadmap for exploiting metabolic dependencies in IDH-driven malignancies. CLINICAL TRIAL NUMBER: Not applicable.
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Journal of Sustainable Metallurgy
·
2026
·Vol. 12
·期 3
·3129-3142
·
IF 3.5
Xu Lei,Liu Aimin,Wang Jinhui,Liu Fengguo,Hu Xianwei,He Zhenkun,Shi Zhongning
Antioxidant coatings are an effective approach to reducing the additional consumption of carbon anodes during molten-salt aluminum electrolysis. In this article, low molar ratio cryolite was used as the coating material, while acrylic, vinyl acetate-ethylene (VAE), polyvinyl alcohol, and sodium carboxymethyl cellulose (CMC) were used as binders. Under laboratory conditions, high-temperature oxidation experiments conducted on coatings prepared with different binders show that the coating formulated with acrylic binder exhibits the best oxidation resistance. Porosity measurements of the sintered coatings indicate that the acrylic-based coating has the lowest porosity. Electrolysis experiments using these coatings further reveal that the acrylic-coated anode exhibits the lowest additional consumption. Scanning electron microscopy (SEM)–energy-dispersive X-ray spectroscopy (EDS) elemental mapping of the initial electrolyte revealed the presence of a small amount of silicon impurity. After electrolysis, X-ray diffraction (XRD) and XRF analyses of the electrolyte indicated that no additional impurity phases were detected. SEM–EDS mapping and X-ray diffraction (XRD) analysis of the cathodic aluminum further confirmed that a trace amount of silicon was present in the product, mainly in the form of aluminum silicate. High-temperature oxidation experiments and electrolysis tests consistently verified that the coating provided effective protection to the anode, while no deterioration in the purity of the primary aluminum was observed. Graphical
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Zhao Zhenghong,Xu Shuzhen,Xu Yue,Liao Guoming,Pan Changbing,Zhang Lianming,Ye Fanggui,Li Jianping
Molecularly imprinted nanozymes (MINs) offer a compelling alternative to antibody-based assays by integrating specific molecular recognition with enzyme-like catalytic activity. Nevertheless, conventional molecular imprinting of glycoproteins remains technically challenging. In this work, employing CA19-9 as a model glycoprotein biomarker, we develop a surface-directed glycan imprinting strategy by integrating molecular imprinting technology with iron-based metal-organic framework (MIL-88B-NH 2 ) nanozymes. The characteristic glycan of CA19-9 (sialyl Lewis A, SLe a ) is pre-adsorbed onto the amino-rich MIL-88B-NH 2 surface via hydrogen bonding and boronic acid affinity, followed by sol-gel polymerization to grow an imprinted shell. After template removal, the recognition cavities are automatically positioned within nanometers of the intrinsic Fe catalytic sites. Upon specific binding of CA19-9, steric hindrance exerted by the protein backbone impedes diffusion of the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) to the surface of MOF, resulting in quantifiable suppression of color development. The developed sensor demonstrates sensitive detection of CA19-9 across a wide linear range from 0.001 to 50 U mL −1 , with a detection limit of 1.01 × 10 −4 U mL −1 (3σ/slope), and shows excellent selectivity in serum samples. This work provides a stable, cost-effective, and visual biosensing platform with significant potential for point-of-care biomarker testing. Graphical
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