APPLIED CATALYSIS B-ENVIRONMENTAL
·
2018
·
IF 11.698
Shanshan Su,Chengjin Cao,Yaping Zhao,Dionysios D. Dionysiou
Roxarsone (ROX) is extensively used as a feed additive which tends to leave toxic inorganic arsenic (As) in the natural environment. A composite of reduced graphene oxide and carbon nanotubes was impregnated with goethite designated as α-FeOOH@GCA and tested as an integrated catalyst and adsorbent for ROX decontamination. Nearly 100% of the ROX was transformed in α-FeOOH@GCA activating persulfate (PS) under UV 365 irradiation system at relatively low dosage of the α-FeOOH@GCA catalyst (250 mg L −1 ) in 120 min. The As(V) released in the ROX oxidation was simultaneously adsorbed onto the surface of the α-FeOOH@GCA. A possible ROX transformation pathway is proposed. The As(V) adsorbed on catalyst was recovered and used to form an efficient Ag 3 AsO 4 photocatalyst. This work further extends the oxidation-adsorption technology in solving organoarsenical contamination without producing secondary pollution.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 136
·期 36
·47937
·
IF 2.188
Shan Jiang,Wei Wang,Wei Chen,Hongwen Zhang,Kemin Wang
Polypropylene (PP)–silicon dioxide (SiO 2 ) compound microporous membranes were fabricated by a melt-stretching method. Although the permeability, porosity, and hydrophilicity values of the microporous membranes were found to be highest at an SiO 2 content of 2 wt %, the heat resistance of the membranes was relatively low. To improve the heat resistance of the microporous membranes, a macromolecular coupling agent, PP- g -poly(α-methyl styrene- co -glycidyl methacrylate- co -γ-methacryloxypropyl trimethoxy silane) (PAGK), was introduced into the membrane. In the PP–PP- g -PAGK–SiO 2 composite systems, the content of SiO 2 was controlled at 2 wt %, and the proportion of PP- g -PAGK was varied. With increasing PP- g -PAGK content to 0.6%, the Gurley value decreased from 250 to 239 s, and the porosity increased from 50.8 to 51.6%. The hydrophilicity of the microporous membranes increased with the incorporation of PP- g -PAGK, and their water vapor transmission rate reached a maximum of 3360 g m −2 /24 h at a PP- g -PAGK content of 0.6%. The heat resistance of the PP–PP- g -PAGK–SiO 2 compound microporous membranes was slightly higher than that of the pure PP microporous membrane. Additionally, the cycle performance of a cell assembled with the PP–PP- g -PAGK–SiO 2 membrane was better than that constructed with the pure PP membrane. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 47937.
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BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Biyan Xu,Delun Zheng,Weiwei Qiu,Feng Gao,Shaoxiong Jiang,Qingxiang Wang
In this work, a novel electrochemical DNA biosensor has been developed based on the hybrid film of fern leaf-like α-Fe 2 O 3 microparticles and chitosan (CS). The fern leaf-like α-Fe 2 O 3 microparticles were synthesized via a facile template-free hydrothermal method, and their morphologies were characterized by X-ray diffraction, energy dispersive spectrometry, scanning electron microscope, and transmission electron microscope. Electrochemical characterization assays revealed that the hybrid film modified electrode had remarkable synergistic effects of the large accessible surface area and high electrical conductivity of semiconductive Fe 2 O 3 , and the good film stability of CS. Based on the rich amino groups on CS, the CS–Fe 2 O 3 hybrid film was employed as a functional matrix for probe DNA immobilization using terephthalaldehyde (TPA) as a bifunctional arm-linker. The hybridization capacity of the developed biosensor was evaluated with electrochemical impedance spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− as the indicating probe. A wide dynamic detection range from 1.0×10 −14 to 1.0×10 −10 M with ultralow detection limit of 5.6×10 −15 M was achieved for the target DNA. The hybridization selectivity experiments further revealed that the biosensor could discriminate fully complementary sequences from one-base mismatched, three-base mismatched, and non-complementary sequences. Moreover, the biosensor showed the advantage of good regeneration ability and reproducibility.
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Analytical Methods
·
2015
·Vol. 7
·期 5
·2155-2161
·
IF 1.821
Hai-Long Li,Zhen-Miao Qin,Hong-Die Cai,Yin-Feng Tan,Xiao-Po Zhang,Yu-Chao Luo,Bei Li,Feng Chen,Jun-Qing Zhang
The evaluation of efficacy and safety should be paralleled with the assessment of comprehensive pharmacokinetic (PK) properties for a drug candidate, and a robust bioanalytical method is a prerequisite for obtaining PK information. α-Hederin is reported to have various in vitro and in vivo activities; however, very little is known about its PK and metabolic characteristics. In this study, we have developed an efficient LC-ESI(−)-MS/MS assay for α-hederin and its sapogenin hederagenin in rat plasma. Sample cleanup involved methanol precipitation for identification analysis and liquid–liquid extraction with ethyl acetate for quantification assay. LC analysis was performed under reversed-phase conditions in the modified “pulse gradient elution” mode. Analyte identification and quantification were conducted using multiple reaction monitoring (MRM) mode with euscaphic acid as the internal standard. Under these conditions, deglycosylated metabolites and their sulfate conjugates were detected; however, hederagenin was not detected in rat plasma samples after both oral and intravenous treatments. The mean plasma clearance (CL), volume of distribution (VSS) and elimination half-life (t1/2) of α-hederin were 0.24 L h−1 kg−1, 0.25 L kg−1 and 2.67 h, respectively. The oral bioavailability (F) of α-hederin in rats was about 0.14%, which might result from the poor intestinal absorption and/or extensive biliary excretion. It is hoped that this validated method will be useful for future PK studies of α-hederin.
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CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jing Tang,Linyuan Liao,Xuexiang He,Lidan Lv,Xiangbiao Yin,Wenlong Li,Yuezhou Wei,Shunyan Ning,Lifeng Chen
212 Pb serves as one of the most important nuclides for targeted alpha therapy (TAT), with good potential in the treatment of malignant tumors. However, medical and clinical applications are seriously limited by its extremely short supply, which strongly depends on the acquisition of its parent nuclides such as 228 Ra or 224 Ra. To address this issue, we conducted a systematic investigation on the separation of trace radium from natural thorium using the ion exchange chromatography method, and a novel porous silica-based composite resin functionalized with sulfonic acid groups was prepared, characterized, and employed. Experimental results showed that the silica-supported resin was successfully prepared with the sulfonic acid groups, and possessed small particle size, mesoporous pore structure and large specific surface area. The resin exhibited high selectivity toward thorium compared with barium, and the separation factor reached 123 at low acidity and at low temperature. The resin demonstrated a fast adsorption speed and an excellent separation performance, and the adsorption mechanism was confirmed based on the ion exchange between the sodium carried and the target cations. On the basis, column experiments were further conducted and finally the separation of trace radium from thorium was realized using a small column filled with about 1.0 g resin. The chemical recovery of radium reached 97 %, and the decontamination factor for thorium exceeded 40,000. This work verifies the feasibility of directly extracting 228 Ra and 224 Ra from natural thorium using the sulfonic acid group functionalized materials in the dilute nitric acid solution, and presents an excellent material and systematic experimental data on the separation activities, rendering this study particularly significant.
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CHIRALITY
·
2025
·Vol. 37
·期 8
·e70050
·
IF 3
Xiangwen Chen,Xuan Chen,Juan Pan,Shuqi Deng,Qiubing Qin,Qiang Fu,Jiliang Cao
In this work, the silica gel bonded amylose[( S )-α-methylbenzyl carbamate] (CHIRALPAK IH) was chosen as the chiral stationary phase (CSP) for the separation of five quinolone enantiomers by high-performance liquid chromatography (HPLC), namely, ofloxacin, flumequine, nadifloxacin, lomefloxacin, and clinafloxacin. The mobile phase composition, organic modifier, and acid–base additive were systematically investigated for the baseline separation of these five interested quinolones. The optimized mobile phases were n -hexane–ethanol–acetic acid–diethylamine (60:40:0.2:0.2, v / v / v / v ) for ofloxacin and nadifloxacin, n -hexane–ethanol–acetic acid–diethylamine (60:40:0.2:0.2, v / v / v / v ) for flumequine, n -hexane–isopropanol–acetic acid–diethylamine (60:40:0.3:0.3, v / v / v / v ) for lomefloxacin, and n -hexane–ethanol–acetic acid–diethylamine (70:30:0.3:0.3, v / v / v / v ) for clinafloxacin. The interaction forces between the amylose CSP and target quinolones were simulated by computerized molecular docking to study their enantiorecognition mechanisms. The results indicated that hydrogen bonding, π–π stacking, and hydrophobic interactions collectively contributed to the stereoselective binding. The differences in these interaction forces between the quinolone enantiomers, particularly the greater contribution of hydrogen bonding in one enantiomer compared to the other, led to a significant difference in binding energy. This differential binding energy ultimately governed the elution order and enabled chiral recognition of the five quinolone enantiomers.
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JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
·
2025
·
IF 5.4
Wei Dai,Liping Dai,Tao Su,Qin Lin,Zhiwen Lin,Shuxin Ye,Peihao Xu,Hongfeng Chen,Xilong Zheng
A green ultrasound-assisted deep eutectic solvent (UAE–DES) method was optimised for extracting flavonoid enzyme inhibitors from Blumea aromatica. Optimal conditions (choline chloride-1,4-butanediol 1:3 molar ratio, 43% water content, 50 mL/g liquid-to-solid ratio, 80 °C ultrasound for 48 min) yielded 3.15% total flavonoids, 45.2% higher than 50% ethanol extraction. Scanning electron microscopy confirmed cell wall disruption. The UAE–DES extract showed the strongest enzyme inhibition among all extracts tested (IC50 35.872 ± 0.294 µg/mL for α-glucosidase, 9.126 ± 0.285 μg/mL for tyrosinase), though the α-glucosidase inhibition was much weaker than acarbose, while tyrosinase inhibition was comparable to kojic acid. Six flavonoids were identified via UPLC-Q-Orbitrap HRMS, including scutellarein and corylin. Molecular docking revealed strong binding affinities (≤ −5 kcal/mol), with corylin showing the highest binding to both enzymes through hydrogen bonds and van der Waals interactions. This approach supports sustainable discovery of natural enzyme inhibitors for antidiabetic and skin-whitening applications.
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Journal of Water Process Engineering
·
2024
·
IF 6.3
Hongshan Jia,Yingqing Zhan,Fei Zhu,Ximin Chen,Yajie Lei,Xinyue Duan,Jie Liu
In the practical purification of oily wastewater, the application of polymer-based membrane is greatly restricted due to the oil adhesion and complicated fouling problems. Herein, to conquer the aforementioned challenges, we prepared the polyphenol mediated Photo-Fenton self-cleaning and superhydrophilic/underwater superoleophobic α-FeOOH/g-C 3 N 4 @PVDF composite membrane via simple vacuum-assisted self-assembly technology. The g-C 3 N 4 nanosheets modified by tannic acid were introduced into the surface of PVDF ultrafiltration membrane, in which the intercalation structure was regulated by the random interpenetration of α-FeOOH nanorods . The abundant hydroxyl and amino groups and the rough micro-nano structure formed by intercalation structure were favorable for the superhydrophilic feature (WCA = 0°, UOCA>150°), thus resulting in ultra-low oil adhesion. Specially, the intercalation structure improved the water permeation channel, further promoting the water/oil separation flux. Therefore, the composite membrane exhibited superior separation flux (452.35–650.21 L m −2 h −1 ) and separation efficiency (>99.06 %) for various surfactant-stabilized emulsions. Moreover, thanks to the Photo-Fenton reaction, the composite membrane showed excellent degradation efficiency on simulated pollutants (various dyes) within 60 min (MeB: 95.55 %, CR: 90.83 %, MO: 98.90 %, CV: 95.11 %), showing satisfactory photocatalytic self-cleaning performance. This work provides a strategy for the potential application of polymer-based membranes in the treatment of complex surfactant-stabilized emulsion wastewater from petrochemical industry.
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JOURNAL OF ORGANIC CHEMISTRY
·
2020
·
IF 4.335
Lin Chen,Jin He
A novel Michael/alkylation cascade reaction of N-unprotected 3-bromooxindoles with α,β-unsaturated acyl phosphonates using DABCO as a robust catalyst followed by the derivatization of the acyl phosphonate intermediates in situ has been developed. This scenario enables rapid access to a diverse set of highly functionalized spirocyclopropyl oxindoles in moderate yields with good to excellent diastereoselectivities, which are analogues of a high active non-nucleoside reverse transcriptase inhibitor against HIV-1. The synthetic potential of this tactic has been highlighted by a gram-scale reaction and Suzuki cross-coupling reactions of the product. Moreover, the reaction mechanism has been tentatively elucidated by control experiments and dynamic high-resolution mass spectrometry studies, which indicates that the Michael/alkylation cascade reaction involves DABCO-derived α-substituted ammonium ylides.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 8
·1-9
·
IF 5.705
Huang Shan,Yang Erli,Yao Jiandong,Liu Yi,Xiao Qi
An ultrasensitive fluorometric assay is described for the determination of the activity of the enzyme α -glucosidase in waters and living cells. Carbon dots doped with nitrogen and boron (N,B-CDs) were prepared that have excitation/emission peaks at 400/510 nm and a fluorescence quantum yield of 47%. 4-Nitrophenylglucoside is added and then hydrolyzed by α -glucosidase to form yellow 4-nitrophenol which screens off fluorescence due to an inner filter effect. The method was applied to the determination of α -glucosidase activity and has a 3 mU mL −1 detection limit. It was subsequently applied to the determination of the α -glucosidase inhibitor acarbose which can be determined in a concentration as low as 10 nM (at three times the standard deviation versus slope). The method was also applied to the determination of α -glucosidase activity and acarbose in living HeLa cells and MCF-7 cells. The method is simple, sensitive, and excellently selective.
N,B-CDs as ultrasensitive fluorescence probe for α -glucosidase activity and its inhibitor in waters and living cells based on IFE.
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ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Yingying Yin,Lei Zhu,Xiao Chang,Jinwei Xue,Shifan Yu,Xiaofang Li,Qingzhong Xue
Designing and constructing a stable water-retention layer acting as the isolation between the oil and membrane surface holds great significance for solving the membrane fouling problems in oil/water separation, including common layered oil/water mixtures, immiscible oil-in-water emulsions, and even high-viscosity crude oil-in-water emulsions. Inspired by the self-cleaning property of sea urchin thorns, a bioinspired anti-oil-fouling hierarchically structured membranes decorated with urchin-like α-FeOOH particles was successfully prepared via the layer-by-layer (LBL) self-assembly method, maintaining numerous effective micro-nanopores. The hierarchical structured membrane exhibited superior superhydrophilicity/underwater superoleophobicity, high water-retention ability, and preferable anti-oil-fouling properties. Furthermore, the biomimetic membrane with controllable pore sizes could not only separate common layered oil/water mixtures but also effectively separate immiscible surfactant-stabilized oil-in-water emulsions of both low-viscosity crude oil and high-viscosity crude oil with an ultrahigh water flux up to 2598.4 L m–2 h–1 and an outstanding separation efficiency of 98.5%, revealing its promising prospect in oily wastewater treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Cailing Yu,Xinyu Zu,Yan Liang,Xiangzhong Zhao,Yingqiu Li,Chenying Wang,Hua Wang
Food-derived dual-effect peptides exhibit multi-target therapeutic potential and represent a promising strategy for managing comorbid conditions. However, technical challenges in their purification remain major obstacles to practical application. In this study, a nanofibrous membrane immobilized with angiotensin I-converting enzyme (ACE) and α-amylase (PPA) was developed for the efficient screening of dual-target inhibitory peptides. The immobilized enzymes achieved maximum loading capacities of 1.0466 mg/cm 2 (ACE) and 2.2871 mg/cm 2 (PPA), while retaining specific activities of 0.0173 U/mg and 0.0208 U/mg, respectively. This approach markedly reduced the number of peptide candidates from 1714 to 234, while enhancing their quality, as evidenced by increased inhibitory activities of 21.85 % for ACE and 22.22 % for PPA. Two dual-target inhibitory peptides, YDPR and LPDYR, were successfully isolated from Chlorella protein hydrolysates. Enzyme inhibition assays confirmed their potent inhibitory effects on ACE (YDPR: IC 50 = 89.05 ± 0.90 μM; LPDYR IC 50 = 170.50 ± 0.68 μM) and PPA (YDPR: IC 50 = 76.30 ± 1.05 μM; LPDYR: IC 50 = 112.51 ± 3.19 μM). Molecular dynamics simulations revealed that the dual inhibitory activities of these peptides arise from the formation of stable enzyme-peptide complexes involving key catalytic residues of ACE (Glu384, Tyr523, His353, Ala354, and Glu411) and PPA (His305, Asp300, Asp356, and Trp59). In summary, the high-efficiency screening strategy developed here provides a solid theoretical and technical foundation for the discovery of multi-target inhibitors.
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