BIOSENSORS & BIOELECTRONICS
·
2025
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IF 10.5
Yuwei Zhang,Jiawen Han,Shuai Qin,Juan Wang,Zhihua Lv,Shaojun Dong,Daoqing Fan
Exploring novel versatile nanozymes for multi-signal biosensing and cellular application is one of the most promising directions to meet the diversified requirements in this field. Herein, by harnessing coordination-propelled self-assembly between Fe (II) and DNAs, we prepared Fe-DNA nanospheres (Fe-DNA NSs) via a cost-effective one-step hydrothermal method, and pioneered the application of its excellent POD-mimicking property to fluorescent substrates. Initially, we investigated its enzyme-like activity using TMB as canonical colorimetric substrate and screened its catalytic oxidation effects towards different fluorescent substrates, such as T-HCl, AR, OPD and Sc, respectively. Afterwards, by virtue of the contrary fluorescent changes of Sc (decreased FI 465 ) and OPD (increased FI 562 ) and the cooperative effects of FRET/IFE between them, we devised the first universal Fe-DNA nanospheres-based ratiometric fluorescent (RF) platform. Taking H 2 O 2 and glucose as model targets, two RF biosensors based on the alternative direct-nanozyme-catalysis and enzyme/nanozyme-tandem-catalysis were rationally fabricated, respectively. And we further exploited them to evaluate the quality of commercial contact lens care solution, and sensitively determine the blood glucose level of human. Moreover, corresponding cytotoxicity experiments adequately proved the superior biocompatibility of Fe-DNA NSs over most inorganic nanozymes. Furthermore, taking Cy5-labelled A20 strands as templates, we synthesized small-sized (∼60 nm) Fe-DNA fluorescent nanozyme and achieved efficient cellular delivery/imaging. This work not only offered a valid prototype for operating multi-signal-responsive nanozymatic biosensors, but also opened unique avenues for the bio-applications of nucleic acids-originated fluorescent nanozymes in cellular imaging and biotherapy.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Ning Zhang,Yuanyuan Song,Danni Wu,Tian Xu,Meiling Lu,Weibing Zhang,Hailin Wang
Crotonaldehyde (Cro) is one of widespread and genotoxic α,β-unsaturated aldehydes and can react with the exocyclic amino group of 2′-deoxyguanosine (dG) in genomic DNA to form 1, N 2 -propano-2′-deoxyguanosine (ProdG) adducts. In this study, two diastereomers of high purity were prepared, including non-isotope and stable isotope labeled ProdG adducts, and exploited stable isotope dilution-based calibration method. By taking advantage of synthesized ProdG standards, we developed a sensitive ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI–MS/MS) method for accurate quantification of two diastereomers of ProdG adducts. In addition to optimization of the UHPLC separation, ammonium bicarbonate (NH 4 HCO 3 ) was used as additive in the mobile phase for enhancing the ionization efficiency to ProdG adducts and facilitating MS detection. The limits of detection (LODs, S/N = 3) and the limits of quantification (LOQs, S/N = 10) are estimated about 50 amol and 150 amol, respectively. By the use of the developed method, both diastereomers of ProdG adducts can be detected in untreated human MRC5 cells with a frequency of 2.4–3.5 adducts per 10 8 nucleotides. Crotonaldehyde treatment dramatically increases the levels of ProdG adducts in human MRC5 in a concentration-dependent manner.
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NEW JOURNAL OF CHEMISTRY
·
2026
·
IF 2.6
Wuhan Sang,Miaomiao Liu,Li Xu,Shilong Yang,Yanqin Wang,Caihua Zhang,Wen Lu
A binuclear Cu(II) complex, [Cu2L2(OAc)4]·H2O (Coupy-Cu), with 3-(pyridin-3-yl) coumarin as the ligand, was synthesized by the slow solvent evaporation method. Complex Coupy-Cu was characterized by elemental analysis and infrared spectroscopy, and its crystal structure was determined by X-ray single-crystal diffraction, revealing a distorted octahedral geometry around each copper center. The antiferromagnetically coupled dimeric structure was further confirmed by EPR and VSM, which indicated a diamagnetic singlet ground state (S = 0) with weak paramagnetic susceptibility. Complex Coupy-Cu exhibited favorable cytotoxic activity against a panel of human cancer cell lines (HeLa, A549, MCF-7, and HepG2), with anti-proliferative efficacy against HepG2 cells superior to that of oxaliplatin and induced 43.3% apoptosis at 20 µM in a concentration-dependent manner. It is worth emphasizing that the complex showed significantly lower toxicity toward normal cells compared to cisplatin and oxaliplatin, displaying a safety index 5.2 times that of oxaliplatin and 30.4 times that of cisplatin. Investigations via electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements, and molecular docking indicated that the complex binds to calf thymus DNA (CT-DNA) primarily through an intercalative mode. This binding was further validated via molecular dynamics simulations, which demonstrated stable complex formation and a calculated binding free energy of −13.74 kcal mol−1, driven predominantly by van der Waals interactions.
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
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IF 10.753
Haowei Fu,Xiaofang Zhu,Qiannan Di,Jiahui Sun,Qianqian Jiang,Qian Xu
Di(2-ethyl-hexyl) phthalate (DEHP), an environmental endocrine disruptor, can destroy the sperm genomic integrity and impairs spermatogenesis. N6-methyladenosine (m6A) is involved in the cellular effects of DEHP. However, the genotoxic effect of DEHP on spermatocytes and the possible role of m6A in this process remain unclear. This study demonstrated that m6A alleviates DEHP genotoxicity in GC-2 cells. In DEHP-treated mice, DNA double-strand breaks (DSBs) were induced in the testis and spermatocytes. To further explore the molecular mechanism of DEHP genotoxicity on spermatocytes, GC-2 cells were exposed to DEHP. DEHP produced distinct genotoxicity and caused DSBs, which led to the inhibition of DNA synthesis and cell cycle arrest. The DNA damage response (DDR) was initiated to repair the DSBs induced by environmentally relevant levels of DEHP (100 μM and 200 μM). During this process, METTL3 upregulated m6A, which facilitated the DDR via stabilizing the DNA damage repair factors ( Rad51 and Xrcc5 ) mRNA to maintain the pro-survival state. Moreover, Mettl3 knockdown partially inhibited DDR. Interestingly, high-dose DEHP (400 μM and 600 μM) directly induced apoptosis rather than the pro-survival state. Altogether: METTL3-mediated m6A participates in maintaining the pro-survival state by upregulating DDR, providing guidance for mitigating the genotoxicity of environment-related level DEHP exposure.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
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IF 7.129
Yi Ning,Xiaoqi Wang,Shiwu Liu,Ling Li,Fangguo Lu
Herein, a graphene oxide (GO)-based fluorescence aptasensor was developed for the sensitive and selective detection of chloramphenicol (CAP), based on exonuclease III (Exo III)-assisted target recycling and Nb.BbvCI-driven DNA walker cascade amplification. Interactions between CAP, hairpin1(HP1), hairpin2 (HP2), and 3′-amino modified hairpin3 (HP3) labeled with carboxyfluorescein (FAM) and covalently coupled to GO enabled efficient CAP detection. CAP was quantitatively assayed by measuring fluorescence at excitation/emission wavelengths of 480/514 nm, resulting from the accumulation of released FAM. A good linear range of 1 fM to 1 nM and a limit of detection (LOD) of 0.875 fM (signal-to-noise ( S/N) = 3) were achieved. This aptasensor can distinguish the CAP from interference antibiotics with good specificity and selectivity, even if the concentration of the interfering substance is ten-fold higher than the target concentration. Moreover, the developed fluorescence aptasensor was successfully applied for the detection of CAP in spiked milk and honey samples. Thus, this method is potentially applicable for assaying CAP in foods and provides a promising strategy for the development of fluorescence aptasensors for environmental sample analysis.
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高影响因子
DALTON TRANSACTIONS
·
2022
·Vol. 51
·期 29
·10930-10942
·
IF 4.569
Yixuan Wu,Dandan Zhao,Jinting Shang,Wenxin Huang,Zhanfen Chen
Polynuclear platinum(II) complexes represent a class of great prospective Pt-based antitumor drugs that may expand the antitumor spectrum and overcome the clinical problems of drug resistance and side effects of platinum-based drugs. Herein, a novel star-shaped trinuclear platinum(II) complex [Pt3(L–3H)Cl3] (1, L = 2,4,6-tris[(2-hydroxybenzyl)(2-pyridylmethyl)amine]-1,3,5-triazine) and its monomer [Pt(L′–H)Cl] (2, L′ = (2-hydroxybenzyl)(2-pyridylmethyl)amine) were synthesized and characterized. The in vitro antiproliferative activities of complexes 1 and 2 against a panel of human cancer cell lines including MDA-MB-231 (triple-negative breast cancer, TNBC), MCF-7 (breast), HepG-2 (liver), and A549 (lung) were investigated. The results revealed that 1 exhibited much higher antiproliferative properties than its monomer 2 against the tested cell lines. Importantly, 1 possessed 3.3-fold higher antiproliferative activity as compared with cisplatin against the TNBC cell line MDA-MB-231. Another TNBC cell line MDA-MB-468 is also sensitive to 1. The results indicated that 1 might have the potential to act as a candidate for the treatment of TNBC. Cellular uptake and distribution studies showed that 1 could pass through the membrane of cells and enter into cells and mainly accumulate in the nuclei and mitochondria. 1 could bind to DNA in a cooperative groove-electrostatic-platinating binding mode and induce stronger DNA double-strand breaks (DSBs) and damaging effects on MDA-MB-231 than cisplatin (upregulation of γ-H2AX). Moreover, the DNA damage could not be easily repaired (upregulation of p53), which would exert a much positive influence on the overcoming of drug resistance. Additionally, flow cytometry studies showed that 1 arrested the cell cycle in the G0/G1 phase, induced mitochondrial membrane depolarization, increased ROS generation, and induced cell apoptosis. The results demonstrated that 1 could target simultaneously mitochondria and nuclei that gave rise to mitochondrial injury and DNA damage and ultimately efficiently promote the apoptotic death of tumor cells. Further mechanistic studies showed that 1 induced MDA-MB-231 cell apoptosis via the p53-mediated mitochondrial pathway by upregulating Bax and cytochrome c and downregulating Bcl-2 proteins, leading to the activation of caspase-3 and upregulation of the cleaved-PARP level. Taken together, 1 with such a synergic mechanism has great potential to be an effective anticancer agent that can overcome treatment resistance in TNBC.
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ACS Applied Materials & Interfaces
·
2021
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IF 9.229
Bo Yao,Jing Zhang,Zhenqiang Fan,Yuedi Ding,Bin Zhou,Runlin Yang,Jianfeng Zhao,Kai Zhang
The detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is crucial for preventing and controlling infectious diseases and disease treatment. In this work, a Au@Ti3C2@PEI-Ru(dcbpy)32+ nanocomposite-based electrochemiluminescence (ECL) biosensor was rationally designed, which realized sensitive detection of the RNA-dependent RNA polymerase (RdRp) gene of SARS-CoV-2. In addition, a DNA walker was also used to excise the hairpin DNAs under the action of Nb.BbvCI endonuclease. Furthermore, model DNA–Ag nanoclusters (model DNA–AgNCs) were used to quench the initial ECL signal. As a result, the ECL biosensor was used to sensitively detect the SARS-CoV-2 RdRp gene with a detection range of 1 fM to 100 pM and a limit of detection of 0.21 fM. It was indicated that the ECL biosensor had a great application potential for clinical medical detection. Furthermore, the DNA walker amplification also played a reliable candidate strategy for other detection methods.
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PHYTOMEDICINE
·
2023
·
IF 7.9
Xi Chen,Ning Zhu,Yajie Wu,Ye Zhang,Yuxuan Zhang,Kaiwen Jin,Zhiyi Zhou,Guang Chen,Jiabing Wang
Background Sorafenib (Sora), a multi-target tyrosine kinase inhibitor, is widely recognized as a standard chemotherapy treatment for advanced hepatocellular carcinoma (HCC). However, drug resistance mechanisms hinder its anticancer efficacy. Derived from Withania somnifera , Withaferin A (WA) exhibits remarkable anti-tumor properties as a natural bioactive compound. This study aimed to examine the mechanisms that underlie the impacts of Sora and WA co-treatment on HCC. Methods Cell proliferation was evaluated through colony formation and MTT assays. Flow cytometry was employed to determine cellular apoptosis and reactive oxygen species (ROS) levels. The evaluation of apoptosis-related protein levels, DNA damage, and endoplasmic reticulum stress was conducte utilizing IHC staining and western blotting. Moreover, the caspase inhibitor Z-VAD-FMK, ATF4 siRNA, ROS scavenger N-acetyl cysteine (NAC), and TrxR1 shRNA were used to elucidate the underlying signaling pathways. To validate the antitumor effects of Sora/WA co-treatment, in vivo experiments were ultimately executed using Huh7 xenografts. Results Sora/WA co-treatment demonstrated significant synergistic antitumor impacts both in vivo and in vitro . Mechanistically, the enhanced antitumor impact of Sora by WA was achieved through the inhibition of TrxR1 activity, resulting in ROS accumulation. Moreover, ROS generation induced the activation of DNA damage and endoplasmic reticulum (ER) stress pathways, eventually triggering cellular apoptosis. Pre-treatment with the antioxidant NAC significantly inhibited ROS generation, ER stress, DNA damage, and apoptosis induced by Sora/WA co-treatment. Additionally, the inhibition of ATF4 by small interfering RNA (siRNA) attenuated Sora/WA co-treatment-induced apoptosis. In vivo , Sora/WA co-treatment significantly suppressed tumor growth in HCC xenograft models and decreased TrxR1 activity in tumor tissues. Conclusion Our study suggests that WA synergistically enhances the antitumor effect of Sora, offering promising implications for evolving treatment approaches for HCC.
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ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Qi Zhang,Qiuyue Zhao,Mingxuan Fu,Xinyu Fan,Haijun Lu,Haiyang Wang,Yufan Zhang,Huan Wang
In this work, carbon quantum dots (CQD) encapsulated in super small platinum nanocrystals core-shell architecture/nitrogen doped graphene hybrid nanocomposite (CQD@PDA@PtNCs-NGR) was design synthesized. Without using any capping reagent, stabilizer and surfactant, very small CQD was served as template and anchoring point for the synthesis of Pt NCs with a super small size (2.25 nm) and a uniform distribution. Meanwhile, dopamine (DA) was used as bridging agent, positioning agent and weak reducing agent to make Pt 2+ grow on the CQD. Combine the high dispersed Pt NCs with high specific surface area and high conductivity of NGR, the CQD@PDA@PtNCs-NGR shows excellent electrocatalytic performance towards the biosensing of DNA damage biomarker- 8-Hydroxy-2′-deoxyguanosine (8-OH-dG). A very low detection limit of 0.45 nM and 0.85 nM (S/N = 3), a wide linear range of 0.013 μM–109.78 μM and a high sensitivity of 7.912 μA μM −1 cm −2 and 4.190 μA μM −1 cm −2 were obtained. The fabricated CQD@PDA@PtNCs-NGR realized the detection of 8-OH-dG in human urine practical sample. Furthermore, CQD@PDA@PtNCs-NGR was applied for the determination of 8-OH-dG generated from damaged DNA and damaged guanine (G), respectively. This work effectively combines the electrochemical signal of 8-OH-dG with DNA damage, confirms the mechanism of DNA damage, which might pave a new way to establish the associations between degree of DNA damage and 8-OH-dG.
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MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 11
·4417-4424
·
IF 4.58
Qu Fei,Liu Yining,Kong Rongmei,You Jinmao
The authors report that the fluorescence of polymer-capped silver nanoclusters (AgNCs) is quenched by MoS 2 nanosheets. However, in the presence of electrolytes such as sodium acetate (50 mM), the nanosheets tend to aggregate, and this causes fluorescence to be restored. If, however, ssDNA is added, it will be adsorbed on the surface of the MoS 2 nanosheets and stabilize them against aggregation. As a result, the emission of the AgNCs (best measured at excitation/emission wavelengths of 375/455 nm) is quenched again. If complementary oligonucleotide is added, a dsDNA will be formed that does no longer prevent aggregation and fluorescence is restored. Similarly, if an aptamer is adsorbed on the MoS 2 nanosheets and its substrate is then added to form a folded state, the fluorescence of the AgNCs also will be restored. As a proof of concept, the DNA sequences of hepatitis B virus and of a dopamine-selective aptamer were chosen as models. The resulting assays have low detection limits and linear ranges that extend from 5 to 30 nM for hepatitis B DNA, and from 0.3 to 1 μM for dopamine. In our perception, this method represents a universal platform for the detection of analytes for which complementary oligomers or aptamers are available.
The blue fluorescence of AgNCs is quenched by MoS 2 nanosheets. MoS 2 nanosheets possess different affinity toward single-stranded DNA (ssDNA) versus double-stranded (dsDNA) and folded DNA. Based on this, multiple targets can be detected through changing the sequence of ssDNA.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-13
·
IF 5.3
Wu Qianyue,Yang Beiying,Wang Yutang,Xiong Yanyan,Zhang Jinhua,Wen Yangping,Rajkovic Andreja
An accurate, sensitive, low-cost, portable, and easy-to-use method for the quantitative detection of Clostridium perfringens (Cp), a zoonotic pathogen widely found in nature and capable of spreading through contaminated food or environments, is essential for epidemiology, prevention, and diagnostics. Here, we have designed a smartphone-based electrochemical DNA biosensor, which utilizes carboxyl-functionalized multi-walled carbon nanotubes (COOH-MWCNTs) as the substrate, L-cysteine (L-Cys) as the linker, and a synergistic modification of the bioelectrode with gold nanoparticles (AuNPs) and polyamidoamine dendrimers (PAMAM). This design enables highly sensitive, low-cost, label-free, and portable detection of the cpb2 gene in Cp. The sensor not only enhances detection performance but also improves convenience and practicality. Differential pulse voltammetry (DPV) was used to monitor the electrochemical signal response to changes in target DNA concentration, enabling the tracking of the DNA hybridization process. Under optimized conditions, the biosensor exhibited a linear detection range from 10⁻ 14 to 10⁻⁸ M, with a detection limit of 1.5 fM. It demonstrated excellent selectivity for the cpb2 gene and was successfully applied to detect variations in cpb2 gene content in wastewater and fecal DNA samples. Compared with the traditional method, the detection time of this method is short, the operation of professional and technical personnel is not required, the instrument is small and portable, and the single detection cost is significantly reduced. This study provides a new strategy for the rapid, portable, and highly sensitive detection of bacterial toxin genes in livestock and aquaculture. Graphical
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ANALYST
·
2017
·Vol. 142
·期 15
·2781-2785
·
IF 3.885
Zhenzhen Chen,Yaxin Niu,Guiying Cheng,Lili Tong,Guanglu Zhang,Feng Cai,Tingting Chen,Bao Liu,Bo Tang
The development of fast, sensitive, selective and flexible methods for the detection of iodide is highly demanded and is of great significance. In this work, single-stranded DNA-templated copper nanoparticles (ssDNA-CuNPs) generated by sodium ascorbate reduction of Cu2+ along the single-stranded DNA of poly-T were utilized as a fluorescent probe for the determination of iodide ions (I−). The detection scheme is based on the instant quenching of the fluorescence of ssDNA-CuNPs by iodide ions. I− can be quantified in the concentration range from 0.050 to 40 μM and from 40 to 80 μM, and the limit of detection is as low as 15 nM. This method provides a simple and convenient strategy for the biochemical assay of I−, which is also helpful for early diagnosis of related diseases. The establishment of a low cost and fast detection method would be particularly important in developing countries where medical supplies are lacking.
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