JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Jinmiao Wang,Yanwen Liu,Cheng Yu,Xinmeng Wang,Juan Wang
In vivo monitoring of multiple pesticide contamination is of great significance for evaluating the health risks of different pesticides, agricultural production safety, and ecological and environmental assessment. Here, we report a hydrogel microneedle array coupled light-addressable photoelectrochemical sensor for tracking multiple pesticide uptake and elimination in living animals and plants, holding three prominent merits: i) enables in-situ detection of in vivo pesticides, avoiding cumbersome and complex sample transportation and handling processes; ii) allows repeated in vivo sampling of the same organism, improving tracking test controllability and accuracy; iii) avoids lethal sampling, providing a better understanding of the pesticides fate in living organisms. The coupled sensor is mechanically robust for withstanding more than 0.35 N per needle and highly swellable (800 %) for timely extraction of sufficient in vivo solution for analysis. For proof-of-concept, it achieves in-situ detection of atrazine, acetamiprid, and carbendazim efficiently and quantitatively in artificial agarose skin models, mouse skin interstitial fluids, and plant leaves with little inflammatory reaction. This simple, highly integrated, minimally invasive, and high-throughput in vivo monitoring method is ideal for future field environmental monitoring and plant and animal disease diagnosis.
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Food Bioscience
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2025
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IF 5.9
Jiayue Tang,Dan Yao,Jiahe Zhao,Maolin Tu
Human milk oligosaccharides (HMOs) are significant constituents of breast milk and have potential anti-inflammatory bioactivities. We compared the immunomodulatory effects of lacto-N-biose I (LNB), lacto-N-triose II (LNT II), galacto-oligosaccharides (GOS), and their combinations (GOS+LNB, GOS+LNT II, LNB+LNT II, m/m=1:1) in lipopolysaccharide-induced RAW264.7 macrophages. LNT II and LNB+LNT II efficiently inhibited inflammation-related gene expression and enhanced phagocytosis and anti-inflammatory ability, which may be attributed to their N-acetylglucosamine structure. Three milk powder prototypes (control, LNT II, and LNB+LNT II) were formulated and characterized, exhibiting favorable physicochemical properties. In-vitro simulated digestion coupled with caucasian colon adenocarcinoma (Caco-2) inflammation model demonstrated that the milk powder digestives inhibited tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) release while promoting interleukin-10 (IL-10) production. The digestives of the three milk powders could also inhibit the decrease in transmembrane electrical resistance and the increase in intestinal permeability, playing a crucial role in maintaining intestinal homeostasis. This study will contribute to the functional evaluation and product development of HMOs and inform milk powder design.
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JOURNAL OF LIPOSOME RESEARCH
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2021
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IF 3.648
Hua Nie,Bo Qiu,Qi-Xuan Yang,Ying Zhao,Xiao-Min Liu,Ying-Ting Zhang,Fu-Lin Liao,Sheng-Yuan Zhang
In this study, we describe a novel synthesis of galactosylated lipids by lipase catalysis. Lactitol (Lac), galactose (Gal), or N-acetyl galactosamine (GalNAc) was coupled with cholesterol (CHS) as target head groups by enzyme-catalyzed regioselective esterification to produce three kinds of lipids: CHS-1-Gal, CHS-6-Gal, or CHS-6-GalNAcFootnote1. The biological effects of galactosylated lipids carrying different constitutional isomers of the pendent sugar species were investigated. LP-1-Gal (liposomes containing 5.0 molar% of CHS-1-Gal) showed strong binding to tetrameric lectins of Ricinus communis agglutinin (RCA120) in vitro, while LP-6-Gal (liposomes containing 5.0 molar% of CHS-6-Gal) and LP-6-GalNAc (liposomes containing 5.0 molar% of CHS-6-GalNAc) did not. After intravenous injection, LP-6-GalNAc, LP-1-Gal and LP-6-Gal rapidly disappeared from the blood and accumulated rapidly in liver (up to 74.88 ± 4.11%, 58.67 ± 5.75%, and 47.66 ± 4.56% of injected dose/g organ within 4 h, respectively). This is significantly higher than the uptake of unmodified liposomes (Unmod-LP) (18.67 ± 6.07%). Pre-injection of asialofetuin significantly inhibits liver uptake of Gal-liposomes (P < 0.01), with the degree of inhibition appearing in the following order: LP-6-GalNAc (73.29%) > LP-1-Gal (67.06%) > LP-6-Gal (53.61%). More importantly, LP-6-GalNAc was preferentially taken up by hepatocytes and the uptake ratio by parenchymal cells (PC) and nonparenchymal cells (NPC) (PC/NPC ratio) was 11.03 higher than LP-1-Gal (7.32), LP-6-Gal (5.83) and Unmod-LP (2.39). We suggest that liposomes containing the novel galactosylated lipid CHS-6-GalNAc have potential as drug delivery carriers for hepatocyte-selective targeting.
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CHEMOSPHERE
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2025
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IF 8.1
Siyu Liu,Xinyue Zhao,Yaobin Ding,Lina Wang,Yiyang Li,Jun He,Chengjun Wang
Water microdroplets are ubiquitous in atmospheric environment, where photo-irradiation and O 3 are available, while photo-decomposition of trace organic pollutants in microdroplets and the impact of atmospheric environmental factors on the photo-chemistry remain unclear. In this study, photochemical generation of reactive oxygen species and degradation of typical endocrine disturbing compounds (EDCs) in microdroplets were investigated under various atmospheric conditions (O 3 , inorganic anions and fulvic acid). The experimental results demonstrate that EDCs can be degraded in microdroplets, and the degradation efficiency is further improved under the stimulated solar irradiation. The degradation efficiency of estrone (E1), estradiol (E2), ethinyl estradiol (EE2), testosterone (T) exposing to sunlight irradiation is 55.45 %, 64.11 %, 89.72 %, and 24.72 %, respectively. The degradation of EDCs is attributed to the generation of hydroxyl and superoxide radicals at or near the microdroplets interface. In addition, with O 3 , the degradation efficiency of EDCs in microdroplets increased to 100 %, 100 %, 100 %, and 83.87 %, respectively. Common inorganic ions (Cl − , NO 3 − , SO 4 2− , and HCO 3 − ) and fulvic acid exhibit positive effects for degradation of EDCs at varying extents. Overall, these findings shed light on the generation of reactive oxygen species and degradation patthways of trace EDCs in microdroplets and improve the understanding of the effect associated with relevant environmental factors in atmospheric microdroplets.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
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IF 6
Xiaoxiao Wang,Jun Chen,Qin Geng,Xuemei He,Yayuan Tang,Lizhen Deng,Ti Li,Chengmei Liu,Taotao Dai
The polyphenols and plant proteins are increasingly being utilized as functional food ingredients, it is critical to understand their interaction mechanism with each other. In this study, protein composites (RP-PP and RP-SPI) were fabricated using pH-driven technology by rice protein (RP) with pea protein (PP) and soy protein isolates (SPI), respectively. The interaction mechanism between the protein composites and proanthocyanidins (PA) was also explored using a combined approach of spectroscopy and thermodynamics. The analysis of fluorescence spectroscopy and turbidity confirmed the formation of the protein composites-PA colloidal complexes. The thermodynamic results calculated from fluorescence data and ITC analysis indicated the hydrogen bond was the main interaction force between the protein composites and PA. The secondary structures of the protein composites were changed with the decreased α -helix and β -turn due to the increasing PA. In addition, the existence of PA reduced the surface hydrophobicity, particle size, and the interfacial tension of proteins. Furthermore, the thermal stability and zeta potential of proteins increased after the binding of the PA. Ultimately, the PA showed a potential in reducing the dimension of droplet and enhancing storage stability of proteins-PA emulsion. This study provides useful information for the industrial application of the proteins-polyphenols complexes.
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2025
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IF 4.7
Qiqi Yang,Yuan Yao,Junping Wang,Mengdi Pang,Jinyan Wu,Fusheng Yin,Jun He,Yanyan Wang,Weidong Chen
Non-small cell lung cancer (NSCLC) is characterized by a complex pathogenesis and high mortality. Targeted drug therapy represents a precise method of treatment. However, Improving sensitivity to targeted agents is currently a major challenge in clinical tumor therapy. Neogambogic acid (NGA), a bioactive compound isolated from the Traditional Chinese Medicine (TCM) Gamboge, has shown potential in NSCLC treatment. Nonetheless, the mechanism by which NGA enhances the sensitivity of NSCLC to the targeted drug Almonertinib remains unclear. This study aimed to elucidate the mechanisms underlying the ability of NGA to enhance the sensitivity of NSCLC to Almonertinib, revealing the synergistic effect exerted by the combination therapy. We established a subcutaneous grafted tumor model of mouse lung cancer by injecting Lewis cells, and evaluated the therapeutic efficacy in vivo . The study demonstrated that NGA combined with Almonertinib effectively suppressed NSCLC tumor growth by promoting cancer cell apoptosis and reducing serum tumor marker levels. In vitro experiments revealed that this combination synergistically inhibited lung cancer cell proliferation and induced apoptosis. Mechanistically, NGA demonstrated the ability to bind to Pregnane X receptor (PXR), regulating the expression of drug-metabolizing enzymes and transporters. We further validated this effect using PXR agonists and inhibitors in A549 cells. Additionally, the combination of NGA with Almonertinib significantly inhibited aberrant EGFR activation and downstream PI3K/AKT signaling, leading to enhanced apoptosis. This study demonstrated that the combination of NGA and Almonertinib inhibited the expression of CYP3A4, P-gp, and BCRP via PXR modulation. Meanwhile, it inhibited the EGFR/PI3K/AKT pathway and enhanced the sensitivity of NSCLC to Almonertinib, exerting synergistic anti-tumor effects. Our findings suggest a promising strategy for integrating active TCM components with targeted therapy in NSCLC management.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
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IF 12.6
Zhe Zhang,Guangyu Jia,Qi Wang,Yamei Yu,Xiaolong Tang,Heqing Zheng,Xinyu Yang,Yao Xiao,Yangrui Ou,Jingjing Jiang,Hua Guo,Ye Wang,Shiyong Li
Intranasal drug delivery via nanocarriers has long been a research focus for enhancing drug concentration in the brain. However, the strategy of exploiting blood–brain barrier (BBB) alterations in atherosclerotic mouse models to enhance nanoparticle-mediated delivery of bromocriptine to the hypothalamus for the treatment of prolactinomas with hyperprolactinemia has not yet been reported. This study reveals that in patients with prolactinomas complicated by arteriosclerosis, bromocriptine therapy more effectively attenuates postoperative elevations in prolactin levels. In a mouse model, liposome-encapsulated bromocriptine efficiently traversed the nasal mucosa and entered the intracranial space. Compared with normal mice, bromocriptine-loaded liposomes delivered higher bromocriptine concentrations to the hypothalamus. Single-cell RNA sequencing revealed a significant upregulation of organic anion-transporting polypeptide 1a4 (Oatp1a4) expression in the brain endothelial cells of atherosclerotic mice. Importantly, bromocriptine-loaded liposomes more effectively reduced prolactin levels in a mouse model of prolactinoma with concurrent atherosclerosis. This study provides a theoretical foundation for the precision treatment of prolactinomas in arteriosclerosis.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Jiacheng Wang,Yuhang Zhang,Yingbin Xu,Hongyu Jin,Can Yang,Hongxing Liu,Yongbo Bao
The blood clam ( Anadara granosa ) exhibits innovative red blood compared to other molluscs, with erythrocytes constituting 79 % to 89 % of the total hemocyte count. Previously, we identified that GATA-binding protein 1 (designated as Ag GATA1) was significantly up-regulated in the transcriptomes of blood clam populations with high hemocyte counts. However, the function of Ag GATA1 in erythropoiesis remains unknown. In this study, we conducted GATA1 knockdown experiments in A. granosa , which resulted in a significant reduction of both erythrocytes and other cells during the S phase, as well as significantly decreased proliferative activity in the gill tissue. The cell proliferation assay demonstrated that overexpression of Ag GATA1 increased the DNA replication activity of K562 cells. Additionally, o-dianisidine staining revealed defects in erythropoiesis within the gill tissue following Ag GATA1 knockdown, while protein analyses confirmed a significant reduction in Ag GATA1 expression. Furthermore, decreased cytoskeletal fluorescence and morphological changes in erythrocytes suggest that Ag GATA1 influenced cytoskeletal protein expression, leading to changes in erythrocyte morphology. This study provides new insights into the role of Ag GATA1 in hemocyte formation and highlights its significance in understanding erythropoiesis within invertebrate developmental tissues.
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Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Shuya Rui,Na Liu,Lingwei Ren,Yang Liu,Zian Liu,Binghui Wu,Hua Pan,Zhiping Ye
Traditional Mn-Ce catalysts for selective catalytic reduction (SCR) of NO with CO (CO-SCR) in iron and steel flue gases are often hindered by competitive adsorption of NO and CO, which compromises catalytic efficiency. In this study, we present a Ni-doped Mn-Ce catalyst that overcomes these challenges, achieving 96 % NO conversion, 98 % N 2 selectivity, and 44 % CO conversion at 175 °C. Comprehensive characterization of the catalyst reveals that Ni doping significantly enhances Mn-Ce interactions, boosting oxygen vacancy density and promoting efficient redox cycling between Mn 3 + /Mn 4+ and Ce 3+ /Ce 4+ . Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) confirms preferential NO adsorption at oxygen vacancy sites, minimizing CO-NO competition and stabilizing the Langmuir-Hinshelwood mechanism, where both gases co-adsorb and react at oxygen-rich vacancies. Mechanistic insights suggest that Ni facilitates electron transfer, weakening the N–O bond in NO and accelerating its dissociation, resulting in faster NO reduction. This study establishes Ni doping as a promising strategy for improving the low-temperature performance of Mn-Ce-based CO-SCR catalysts, paving the way for more efficient catalytic systems.
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TOXICOLOGY AND APPLIED PHARMACOLOGY
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2025
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IF 3.4
Hualu Wu,Haoxin Fu,Fangyuan Xia,Jun Wu,Peiqi Wang,Lu Cao,Ren-ai Xu,Lu Shi
Cariprazine is an antipsychotic medication that has been approved for the treatment of schizophrenia and manic or mixed episodes. Patients with tumors frequently develop psychiatric disorders, necessitating the combination of antitumor and antipsychotic drugs. The objective of the present study was to examine the inhibitory impacts of three antitumor drugs (olmutinib, napabucasin and ponatinib) on the metabolism of cariprazine, and the molecular docking of cariprazine and ponatinib in relation to CYP3A4 was also evaluated. As the results, the half-maximal inhibitory concentration (IC 50 ) values of ponatinib and olmutinib in vitro were < 10 μM, whereas napabucasin was >20 μM. Among these, ponatinib exhibited the smallest IC 50 value. The metabolic stability of cariprazine was observed in the presence or absence of ponatinib in rat liver microsomes (RLM). The IC 50 shift experiments demonstrated that the inhibition of cariprazine by ponatinib was non-time-dependent. In addition, ponatinib was shown to inhibit cariprazine in a mixed manner (RLM) and a competitive manner (HLM), respectively. In the in vivo study, the co-administration of ponatinib resulted in a significant 0.35-fold increase in both AUC (0-t) and AUC (0-∞) for cariprazine, accompanied by a significant 0.25-fold decrease in the CL z/F . Furthermore, the metabolites desmethyl-cariprazine (DCAR) and didesmethyl-cariprazine (DDCAR) exhibited disparate increases in both AUC (0-t) and AUC (0-∞) . Molecular docking studies had demonstrated that both cariprazine and ponatinib could engage in hydrophobic interactions with residue PHE-304 on CYP3A4. Consequently, when ponatinib is employed in conjunction with cariprazine in a clinical setting, it is imperative to assess the efficacy and adverse effects, and adjust the dosage to attain the optimal efficacy.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
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IF 8.128
Ruyu Wang,Hongji Liu,Xiangfu Meng,Yong Qian,Xingyu Wang,Feng Zhu,Rongrong Nie,Hui Wang
Silver nanoparticles (Ag NPs) have attracted extensive research interest in bioimaging and biosensing due to their unique surface plasmon resonance. However, the potential aggregation and security anxiety of Ag NPs hinder their further application in biomedical field due to their high surface energy and the possible ionization. Here, binary heterogeneous nanocomplexes constructed from silver nanoparticles and carbon nanomaterials (termed as C-Ag NPs) were reported. The C-Ag NPs with multiple yolk structure were synthesized via a one-step solvothermal route using toluene as carbon precursor and dispersant. The hydrophilic functional groups on the carbon layer endowed the C-Ag NPs excellent chemical stability and water-dispersity. Results showed that C-Ag NPs demonstrated excellent safety profile and excellent biocompatibility, which could be used as an intracellular imaging agent. Moreover, the C-Ag NPs responded specifically to hydroxyl radicals and were expected to serve as a flexible sensor to efficiently detect diseases related to the expression of hydroxyl radicals in the future.
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DRUG DELIVERY
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2017
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IF 6.402
Xiao Hu,Feifei Yang,Yonghong Liao,Lin Li,Lan Zhang
This study investigated cholesterol–polyethylene glycol (PEG) comodified poly (ethyleneglycol)-poly (lactide) nanoparticles (CLS-PEG NPs) as a novel, biodegradable brain drug delivery system and included an evaluation of its in vitro and in vivo properties. To this end, coumarin-6 (C6), a fluorescent probe, was encapsulated into CLS-PEG NPs by an emulsion polymerization method. We reported that the use of CLS-PEG NPs led to a sustained drug release in vitro. Additionally, cell viability experiments confirmed their safety. The uptake and transport of CLS-PEG NPs, by bEnd.3 cells (an immortalized mouse brain endothelial cell line), was significantly higher than that of a control C6 solution. An investigation of the uptake mechanisms of different NP formulations demonstrated that cholesterol modifications may be the primary way to improve the efficiency of cellular uptake, wherein macropinocytosis may be the most important endocytic pathway in this process. An investigation of the transport mechanisms of CLS-PEG NPs also implicated macropinocytosis, energy and cholesterol in bEnd.3 cells lines. Following an intravenous (IV) administration to rats, pharmacokinetic experiments indicated that C6-loaded CLS-PEG NPs achieved sustained release for up to 12 h. In addition, IV delivery of CLS-PEG NPs appeared to significantly improve the ability of C6 to pass through the blood–brain barrier: the concentration of C6 found in the brain increased nearly 14.2-fold when C6 CLS-PEG NPs were used rather than a C6 solution. These in vitro and in vivo results strongly suggest that CLS-PEG NPs are a promising drug delivery system for targeting the brain, with low toxicity.
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