FOOD CHEMISTRY
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2025
·
IF 9.8
Ruixuan Wang,Guangjun Xia,Yuchen Wang,Song Zhang,Zhenying Ge,Lu Yin,Duanduan Zhao,Zhiyan Jin,Hongyan Xu
Natural polysaccharide-based film suffers lower strength which will further decrease by adding essential oil to endow antibacterial activity. Herein, cellulose nanocrystals (CNC) prepared from Pinus Knraiensis Nut skin (PKNS) was incorporated in gum arabic (GA)/chitosan (CS) composite film. PKNS CNC exhibited needle-like shape and higher stability due to zeta potential of lower than −30 mV. After it was added into GA/CS film, the biocompatibility among them was better and the strength, barrier to moisture and hydrophobicity of GA/CS film were improved. Ocimum gratissimum L. essential oil (OGEO) was blended in GA/CS/CNC film to access obvious antibacterial property against E. coli , Salmonella and S. aureus . Compared to Yanbian cattle beef without package and packed with GA/CS and GA/CS/CNC, that packaged by the antibacterial composite film showed the best quality recognized by the redder color, slowest improved pH value, lowest content of thiobarbituric acid reactive substances (TBARS) and Total volatile basic nitrogen (TVB-N).
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JOURNAL OF FISH BIOLOGY
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2024
·
IF 1.7
Leya Qu,Liping Yang,Yiran Wang,Shaoyang Zhi,Mengjuan Zhao,Jinrui Xiong,Xiao Yan,Chaobin Qin,Guoxing Nie
Isthmin-1 (Ism1) plays roles in glucose uptake in mammals as an adipokine. To investigate its role in the glucose metabolism of common carp ( Cyprinus carpio. L), the Ism1 sequence was cloned, and its expression and distribution in tissues were detected. In addition, we prepared and purified the recombinant Ism1 protein using the E. coli expression system and assessed changes in the expression of key genes related to glucose metabolism through both in vivo injection experiments and primary hepatocyte experiments in vitro. The results revealed that the open reading frame of Ism1 was 1377 bp long, encoding 458 amino acids. Similarity analysis indicated that Ism1 exhibited a close evolutionary relationship with goldfish ( Carassius auratus ), sharing 98.35% amino acid similarity. Ism1 was expressed in all tissues of common carp, with the highest level observed in the heart, followed by the gill, head kidney, and hepatopancreas. Distinct patterns of Ism1 expression were identified during the oral glucose tolerance test and long-term high-carbohydrate and high-fat diet feeding experiments. In vivo studies demonstrated that the serum glucose concentration was reduced on treatment with Ism1, accompanied by a significant upregulation of mRNA levels for gk , hk , and pfk genes in hepatopancreas; conversely pepck and g6pase mRNA levels were significantly downregulated in the hepatopancreas under these conditions as well. Furthermore, our primary hepatocyte experiment confirmed that Ism1 could inhibit pepck and g6pase mRNA expression, while promoting gk , hk , and pfk mRNA expression levels. In conclusion, Ism1, in common carp, could participate in the glucose metabolism, which provides essential information for future studies on the function of Ism1.
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ANALYST
·
2016
·Vol. 141
·期 4
·1257-1265
·
IF 4.033
Guoxin Song,Fulin Zhou,Chunli Xu,Baoxin Li
The ability to recognize and quantify the chirality of alpha-amino acids constitutes the basis of many critical areas for specific targeting in drug development and metabolite probing. It is still challenging to conveniently distinguish the enantiomer of amino acids largely due to the lack of a universal and simple strategy. In this work, we report a strategy for the visual recognition of α-amino acids. It is based on the chirality of L-tartaric acid-capped gold nanoparticles (L-TA-capped AuNPs, ca. 13 nm in diameter). All of 19 right-handed α-amino acids can induce a red-to-blue color change of L-TA-capped AuNP solution, whereas all of the left-handed amino acids (except cysteine) cannot. The chiral recognition can be achieved by the naked eye and a simple spectrophotometer. This method does not require complicated chiral modification, and excels through its low-cost, good availability of materials and its simplicity. Another notable feature of this method is its high generality, and this method can discriminate almost all native α-amino acid enantiomers. This versatile method could be potentially used for high-throughput chiral recognition of amino acids.
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Chemistry Africa-A Journal of the Tunisian Chemical Society
·
2021
·Vol. 5
·期 1
·79-87
·
IF 0
Naz Farah,Ciprian Matteo,Luo Zhixiong,Yan Shilin,Iqbal Shahzad Zafar,Zhu Meifang,Chaemchuen Somboon,Verpoort Francis
The synthesis and use of MOF-derived Co/NCF (N-doped carbon framework) material as a heterogeneous catalyst for copolymers synthesis via ring-opening polymerization (ROP) are reported for the first time. The Co/NCF material is applied for the copolymerization of L -lactides (LA) and ε-caprolactone (ε-CL) as monomers via ROP in bulk without any solvent or co-catalyst. The as-prepared catalyst is characterized using XRD, TPD, XPS, SEM, and TEM analytical techniques. At the same time, 1 H and 13 C-NMR spectroscopy were used to analyze the microstructure of the copolymer confirming the random copolymer structure. The average r values calculated from the heterodiad analysis of the 1 H NMR, data were r LA = 0.96 and r CL = 0.93 and L value calculated from the triads analysis of 13 C NMR, data were L LA = 3.17 and L CL = 2.79, respectively. It has been observed that the melting temperature and glass transition temperature of the copolymer increases with higher LA content. The XRD and DSC analyses also confirmed that the as-synthesized copolymer possesses two phases corresponding to the polylactide and polycaprolactone, respectively. Additionally, the activity of Co/NCF is also compared with other Co-based heterogeneous catalysts to demonstrate its ability. Graphic Abstract
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DOI
PEST MANAGEMENT SCIENCE
·
2025
·
IF 3.8
Xiaobo Huang,Nan Hao,Lili Shu,Zhenyang Wei,Jiawei Shi,Yuee Tian,Genqiang Chen,Xinling Yang,Zhiping Che
BACKGROUND The emergence and increasing intensification of insect pest resistance greatly shortens the service life of commercial insecticides. Hence, the continuous development of novel insecticides is extremely necessary. Discovering new insecticide molecules based on natural products with insecticidal activity has been a popular technique and has made outstanding contributions in this field. RESULTS A series of betulin-cinnamic acid-related hybrid compounds was prepared by molecular hybridization through the bridge of acyloxy groups and their chemical structures were unambiguously confirmed. Against A. pisum , compounds 2e , 3a-3e , 3g , 3i , 3j , and 3m showed complete antifeedant activity at 2 mg/mL; compound 5b exhibited 2.7 times the aphicidal activity of the precursor betulin. Against P. xylostella , compound 2l displayed 3.9 times the insecticidal activity of betulin. Meanwhile, some interesting structure–activity relationships were obtained. Furthermore, compound 2l could destroy the ultrastructure of midgut cells and significantly inhibit the activity of α-amylase in P. xylostella larvae. Molecular docking indicated that the lupane skeleton and p -nitrocinnamoyloxy groups played a key role in the binding of compound 2l to α-amylase. CONCLUSION Compounds 5b and 2l could be further optimized structurally as the secondary lead compounds for the discovery of novel insecticide molecules. © 2025 Society of Chemical Industry.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Haoran Wang,Wei Ji,Feng Xiao,Yewen Xiao,Linlin Li,Xia Shu,Hualin Wang,Jun Yin
Over-ripening and spoilage of fruits, driven by excessive ethylene (C 2 H 4 ) production and microbial proliferation, present critical challenges in fruit postharvest preservation. Herein, a multifunctional photocatalytic film, CTC-Arg@CS/HPMC, is developed by incorporating chlorophyll-TiO 2 @carbon sphere (CTC) photocatalyst and L-arginine (Arg) NO donor into chitosan/hydroxypropyl methylcellulose (CS/HPMC) matrix for banana preservation. The distinctive dual heterojunction structure of CTC enhances photocatalytic activity under visible light, promoting reactive oxygen species (ROS) generation that triggers NO release from Arg. Under combined action of ROS and NO, CTC-Arg@CS/HPMC can not only inactivate bacteria with high antibacterial efficiency against Escherichia coli (93 ± 3 %) and Staphylococcus aureus (99 ± 2 %) within 60 min but also delay fruit ripening through C 2 H 4 degradation and inhibition of biosynthesis. In practical application scenario, CTC-Arg@CS/HPMC can effectively extend banana shelf-life by over 100 % at 25 °C, highlighting its potential as an active packaging material for extending the postharvest life of climacteric fruits.
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INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Mujeeb Alam,Danping Jiang,Huan Zhang,Lei Zhang,Faiqa Nadeem,Nadeem Tahir,Chaoyang Lu,Quanguo Zhang
Photo-fermentation offers a promising roadmap for hydrogen production through their ability to harness light energy, however, its lower conversion efficiency is the major bottleneck for its realization. This study investigates the catalytic role of wideband gap nano-photocatalysts (SiC, ZnO, and SnO 2 ) in enhancing hydrogen generation from Arundo donax L. Physio-optical characteristics of SiC, ZnO, and SnO 2 nano-photocatalysts were assessed through SEM, XRD, and FT-IR which confirmed that incorporated nano-photocatalysts are spherical with uniform size distribution and there is no secondary phase. Hydrogen production experiments with varying nano-photocatalyst concentrations revealed that the maximum hydrogen yield of 104 and 98.83 mL/g was achieved with SiC and ZnO, respectively, at loading concentration of 200 mg/L, whereas 69.30 mL/g were observed with 100 mg/L of SnO 2 . The incorporation of SIC, ZnO, and SnO 2 nano-photocatalyst permitted to increase the hydrogen content in overall gas production by 41 %, 37.34 %, and 18.26 % as compared to the control group. The increase in hydrogen production is related to the enhanced e-h pair separation lifetime of SiC, confirmed from PL spectroscopy. Addition of SiC caused a longer availability of electrons that helped to increase the metabolic rate by 38 % and 40.29 % in energy conversion efficiency (ECE). ANOVA results revealed significant differences between the control and maximum values of CHP, HC%, byproducts, and ECE. Tukey pairwise comparisons showed that SiC and ZnO had non-significant differences at 200 mg/L but both significantly outperformed SnO 2 at 100 mg/L. Thus, the selection of suitable catalysts photocatalysts with enhanced e-h pair separation lifetime may offer promising prospects for sustainable energy technologies by enhancing biohydrogen production.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Yaping Zheng,Kangmeng Sun,Xinyuan Sun,Yue Li,Peigen Xiao,Chunnian He
Sea buckthorn is rich in active ingredients, widespread, and has both medicinal and nutritional value. The present comparative study of wild and cultivated species remains insufficient, which is not conducive to their quality control. Therefore, this study aimed to compare the differences of 21 sea buckthorn samples in total phenolic content (TPC), total flavonoid content (TFC), phenolic components content, secondary metabolites, and antioxidant capacity and the fatty acid, to investigate the quality differences of different varieties. The TPC, TFC and antioxidant activity of wild varieties were higher than those of the cultivated. Multivariate statistical analysis revealed large differences in phenolic content, with higher levels of gallic acid and isorhamnetin-3- O -neohesperidin in the wild, whereas the cultivated were characterized by narcissin and kaempferol. These findings provided the scientific basis for the improvement of quality evaluation standards for different varieties and offered new insights for the further development of sea buckthorn resources.
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ACS Agricultural Science & Technology
·
2025
·
IF 2.9
Huiqian Wang,Dongru Wang,Qiuping Zheng,Yuan He,Qian Yang,Jiani Du,Jiawei Wang,Xinhua Zhan
Polyvinyl chloride microplastics (PVC-MPs) pollution is drawing increasing attention, especially due to concerns about the environmental safety of the diverse additives that they contain. To date, few ecotoxicity data are available for PVC MP-derived chemicals. Here, wheat (Triticum aestivum L.) seedling roots were used to comparatively evaluate the toxicity of three commonly studied PVC MP-derived chemicals (2,4-dimethyl-6-s-hexadecylphenol─Irganox 1076 and tris (2,4-ditert-butylphenyl) phosphate─Irgafos 168-ox, and erucamide─Eru). These chemicals were evaluated individually and in combination. Exposure to these chemicals caused dose-dependent reductions in root dry weight (0.39%–19.29%) and root length (0.11%–8.15%). While Irganox 1076 and Eru had minimal impact on root activity and the antioxidant system, Irgafos 168-ox and its mixture induced significant elevations of antioxidant enzymes (SOD, CAT, and APX) activities and antioxidants (AsA and GSH) concentrations in the roots, enhancing the antioxidant level. Our findings indicate that the coexposure of PVC MP-derived chemicals exerts an additive effect on antioxidant response inhibition. Irgafos 168-ox showed stronger effects with the maximum toxic concentration of 10 mg L–1, whether alone or in additive mixtures, due to its higher potential for root accumulation and oxidative stress induction. These results highlight the need for further research into ecological risks of PVC MP-derived chemicals, particularly under combined exposure scenarios.
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Biotechnology Journal
·
2024
·Vol. 19
·期 4
·2300557
·
IF 3.2
Han-Yu Liu,Feng Qian,Hai-Min Zhang,Qian Gui,Yao-Wu Wang,Pu Wang
The halogenase-based catalysis is one of the most environmentally friendly methods for the synthesis of halogenated products, among which flavin-dependent halogenases (FDHs) have attracted great interest as one of the most promising biocatalysts due to the remarkable site-selectivity and wide substrate range. However, the complexity of constructing the NAD + -NADH-FAD-FADH 2 bicoenzyme cycle system has affected the engineering applications of FDHs. In this work, a coenzyme self-sufficient tri-enzyme fusion was constructed and successfully applied to the continuous halogenation of L -tryptophan. Sp FDH was firstly identified derived from Streptomyces pratensis , a highly selective halogenase capable of generating 6-chloro-tryptophan from tryptophan. Then, using gene fusion technology, Sp FDH was fused with glucose dehydrogenase (GDH) and flavin reductase (FR) to form a tri-enzyme fusion, which increased the yield by 1.46-fold and making the coenzymes self-sufficient. For more efficient halogenation of L -tryptophan, a continuous halogenation bioprocess of L -tryptophan was developed by immobilizing the tri-enzyme fusion and attaching it to a continuous catalytic device, which resulted in a reaction yield of 97.6% after 12 h reaction. An FDH from S. pratensis was successfully applied in the halogenation and our study provides a concise strategy for the preparation of halogenated tryptophan mediated by multienzyme cascade catalysis.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Jian-Guo Zhang,Hao-Xiang Wu,Ai Zhang,Kiran Thakur,Fei Hu,Zhao-Jun Wei
This study compared the effects of ultrasound-assisted extraction (UE), Soxhlet extraction (SE), and steam distillation (SD) on volatile organic compounds (VOCs) and flavor profiles of Piper nigrum L. varieties (Vietnamese pepper, VP; Kampot pepper, KP; Dapo pepper, DP). Using electronic nose, GC–MS, and UPLC analyses, UE showed superior retention of VOC diversity and aromatic complexity, while SE maximized piperine retention (e.g., 4551.35 mg/100 g in VP). SD reduced inter-varietal flavor differences, homogenizing volatile characteristics. Despite methodological variations, key aromatic traits of pepper varieties remained distinguishable. KP processed via UE exhibited the highest terpene content (2503.22 mg/100 g), highlighting its aromatic potential. GC–MS data enabled accurate classification of extraction-based flavor profiles via orthogonal partial least squares discriminant analysis. Nine odorants were identified using odor activity value (OAV) and variable importance in projection (VIP). This study provides insight into method-driven flavor differentiation, VOC characterization, and guides strategies for pepper product development.
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Antioxidants
·
2025
·Vol. 14
·期 9
·1109
·
IF 6.6
Yuwen Mu,Bohua Zhang,Shiqi Lv,Fencan Li,Changming Zhao
Jerusalem artichoke ( Helianthus tuberosus L.) is valued for its high inulin content and adaptability to marginal lands. This study investigated the changes in inulin content, antioxidant capacity, polyphenol concentrations, and enzymatic activities of eight cultivars during 60 days of cold storage. Inulin levels ranged from 582.43 g/kg (LZJ006) to 809.70 g/kg (LZJ055), with LZJ047 maintaining the highest content throughout storage. The antioxidant potential, as measured by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, declined across all cultivars, correlating with the reduction in inulin content. The polyphenol content varied significantly, with LZJ119 having 2.17 times more than LZJ010. POD activity increased, while catalase (CAT) and superoxide dismutase (SOD) activities fluctuated during the storage period. Hierarchical Cluster Analysis identified three distinct antioxidant clusters, revealing significant correlations between inulin content and key antioxidant parameters (CAT, FRAP, DPPH). These findings highlight the pivotal role of inulin in preserving the antioxidant system and bioactive properties of Jerusalem artichoke tubers during extended cold storage, providing valuable insights for post-harvest management and cultivar selection.
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