ENVIRONMENTAL RESEARCH
·
2020
·
IF 5.715
Yangqing He,Hongxia Lv,Yuchen Daili,Qian Yang,Lucas Binnah Junior,Dongjie Liu,Haiyang Liu,Zhanying Ma
In this work, a novel cascade system (i.e., SnTCPP/g-C 3 N 4 /Bi 2 WO 6 ) is successfully constructed using stannum (II) meso-tetra (4-carboxyphenyl) porphyrin (SnTCPP) as the key photovoltaic agent for the first time. Visible light driven photocatalytic experiments indicated that wt . 12% SnTCPP and 30% Bi 2 WO 6 codecorated g-C 3 N 4 demonstrates the highest photodecomposition capabilities for levofloxacin and rhodamine B, achieving 85.64% and 93.64% degradation rates, respectively. The dramatically enhanced photocatalytic performance mainly raised from the synergetic co-effects among SnTCPP, g-C 3 N 4 and Bi 2 WO 6 , including: i) the incorporation of SnTCPP extends the visible light response of the binary Bi 2 WO 6 /g-C 3 N 4 heterojunctions , resulting in the highly efficient visible light harvesting; ii) we find that the g-C 3 N 4 not only serves as a promising supporter to trap electrons from Bi 2 WO 6 , but also as an interfacial electron-hole pairs transfer moderator, like “volleyball setter” to facilitate the charges transfer between Bi 2 WO 6 and SnTCPP. The presence of the “setter” endows a cascade system for boosting the photodegradation efficiency of levofloxacin and rhodamine B. This study provides a promising design strategy to construct efficient g-C 3 N 4 based heterojunctions suitable for removing pharmaceutical antibiotics and hazardous dyes from various real wastewaters .
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Pharmaceutics
·
2023
·Vol. 15
·期 5
·1493
·
IF 5.4
Chaojun Song,Yutao Liu,Jinwei Hu,Yupu Zhu,Zhengjun Ma,Jiayue Xi,Minxuan Cui,Leiqi Ren,Li Fan
Highly sensitive staphylococcal enterotoxin B (SEB) assay is of great importance for the prevention of toxic diseases caused by SEB. In this study, we present a gold nanoparticle (AuNP)-linked immunosorbent assay (ALISA) for detecting SEB in a sandwich format using a pair of SEB specific monoclonal antibodies (mAbs) performed in microplates. First, the detection mAb was labeled with AuNPs of different particle sizes (15, 40 and 60 nm). Then the sandwich immunosorbent assay for SEB detection was performed routinely in a microplate except for using AuNPs-labeled detection mAb. Next, the AuNPs adsorbed on the microplate were dissolved with aqua regia and the content of gold atoms was determined by graphite furnace atomic absorption spectrometry (GFAAS). Finally, a standard curve was drawn of the gold atomic content against the corresponding SEB concentration. The detection time of ALISA was about 2.5 h. AuNPs at 60 nm showed the highest sensitivity with an actual measured limit of detection (LOD) of 0.125 pg/mL and a dynamic range of 0.125–32 pg/mL. AuNPs at 40 nm had an actual measured LOD of 0.5 pg/mL and a dynamic range of 0.5 to 128 pg/mL. AuNPs at 15 nm had an actual measured LOD of 5 pg/mL, with a dynamic range of 5–1280 pg/mL. With detection mAb labeled with AuNPs at 60 nm, ALISA’s intra- and interassay coefficient variations (CV) at three concentrations (2, 8, and 20 pg/mL) were all lower than 12% and the average recovery level was ranged from 92.7% to 95.0%, indicating a high precision and accuracy of the ALISA method. Moreover, the ALISA method could be successfully applied to the detection of various food, environmental, and biological samples. Therefore, the successful establishment of the ALISA method for SEB detection might provide a powerful tool for food hygiene supervision, environmental management, and anti-terrorism procedures and this method might achieve detection and high-throughput analysis automatically in the near future, even though GFAAS testing remains costly at present.Keywords:gold nanoparticles;gold nanoparticle-linked immunosorbent assay;staphylococcal enterotoxin B
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Junyan Kuang,Zipeng Xing,Junwei Yin,Zhenzi Li,Qi Zhu,Wei Zhou
Ti 3+ self-doped single-crystalline SrTiO 3−x nanocubes acting as molecular bricks are successfully assembled onto Ti 3+ self-doping single-crystalline TiO 2−x (B) nanorods through an effortless two-step hydrothermal process coupled with an in situ solid-state chemical reduction method. SrTiO 3−x nanocubes act as molecular bricks, which are uniformly assembled onto the surface of TiO 2−x (B) nanorods due to lattice matching. The band gap of the resultant SrTiO 3−x /TiO 2−x (B) sample is ∼2.97 eV, which exhibits excellent photocatalytic performance for the reduction of Cr(VI) and hydrogen production under visible light. The apparent rate constant k value for the photocatalytic reaction of SrTiO 3−x /TiO 2−x (B) for Cr(VI) reduction is ∼8 times higher than that of white TiO 2 (B). The photocatalytic hydrogen production rate for SrTiO 3−x /TiO 2−x (B) is ∼160.2 μmol g −1 h −1 , which is ∼5 times higher than that of white TiO 2 (B). The enhanced photocatalytic activity can be considered to be caused by a synergetic effect of heterojunction formation and the introduction of Ti 3+ self-doping, which can not only facilitate the separation of photogenerated charge carriers between TiO 2−x (B) and SrTiO 3−x , but also broaden the photoresponse from the UV to visible-light region.
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ARCHIVES OF VIROLOGY
·
2024
·Vol. 169
·期 11
·1-10
·
IF 2.5
Jiao Qian,Zhu Shu,Liao Baolin,Liu Huiyuan,Guo Xiaoyan,Wu Lina,Chen Chuming,Peng Liang,Xie Chan
NLR family member X1 (NLRX1) is an important member of the NOD-like receptor (NLR) family and plays unique roles in immune system regulation. Patients with hepatitis B virus (HBV) infection are more likely to have the NLRX1 mutation p.Arg707Cys than healthy individuals. It has been reported that NLRX1 increases the infection rate of HBV in HepG2 cells expressing sodium taurocholate cotransporting polypeptide (NTCP). However, the role of NLRX1 mutation ( p.Arg707Cys ) in hepatitis remains unclear. We constructed Huh7 cells that stably overexpressed NTCP, using LV003 lentivirus. First, wild-type (WT) and mutant (MT) NLRX1 overexpression plasmids were constructed. The MT plasmid contained a point mutation at position 707 of the WT overexpression plasmid. Then, Huh7-NTCP cells were transfected with the WT or MT NLRX1 overexpression plasmid, and subsequent NLRX1 expression was analyzed using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. HBV RNA levels were determined using RT-qPCR. HBsAg and HBcAg levels were confirmed immunohistochemically. Interferon alpha (IFN-α), interleukin 6 (IL-6), and type I interferon beta (IFN-β) levels were determined using enzyme-linked immunosorbent assay kits. p-p65, p-interferon regulatory factor (IRF) 3, and p-IRF7 expression levels were examined using western blot. The interaction of NLRX1 and retinoic acid-inducible gene (RIG)-1/mitochondrial antiviral signaling (MAVS) protein was confirmed by coimmunoprecipitation. The interaction of NLRX1 with IFN-α, IL-6, or IFN-β was analyzed by dual luciferase reporter gene assay. The levels of HBV RNA, HBsAg, and HBcAg in infected cells transfected with the WT NLRX1 or MT NLRX1 expression plasmid were higher than those in the untransfected control group; and these levels were lower in the cells transfected with MT NLRX1 than in those transfected with WT NLRX1. The levels of IFN-α, IFN-β, IL-6, p-p65, p-IRF3, and p-IRF7 were lower in cells transfected with WT NLRX1 or MT NLRX1 than in control cells. The levels of IFN-β, p-p65, p-IRF3, and p-IRF7 were higher in cells transfected with MT NLRX1 than in those transfected with WT NLRX1. Moreover, NLRX1 competitively inhibited RIG1 binding to MAVS, but the mutation in MT NLRX1 reduced this inhibitory effect. In addition, NLRX1 decreased the promoter activity of IFN-α, IFN-β, and IL-6. Our findings revealed that NLRX1 is a regulatory factor that inhibits the anti-HBV ability of hepatocytes and that the mutation p.Arg707Cys in NLRX1 suppresses HBV infection and activates the IFN/nuclear factor κB pathway.
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CURRENT NEUROVASCULAR RESEARCH
·
2017
·Vol. 14
·期 2
·132-140
·
IF 2.298
Zhu Yingchun,Tang Qiqiang,Wang Guopin,Han Ruodong
Background: Tanshinone IIA is a key active ingredient of danshen, which is derived from the dried root or rhizome of Salviae miltiorrhizae Bge. The tanshinone IIA has protective effects against the focal cerebral ischemic injury. However, the underlying mechanisms remain unclear.
Methods: An in vitro model of cerebral ischemia was established by subjecting cultures of hippocampal neuronal cells to oxygen-glucose deprivation followed by reperfusion (OGD/R). The probes of 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester (CMH2DCFDA)
and 5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine,iodide (JC-1) were used to determine the mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production. Western-blot was used to detect the expression of proteins in HT-22 cells.
Results: The results of cell proliferative assays showed that the tanshinone IIA attenuated OGD/Rmediated neuronal cell death, with the evidence of increased cell viability. In addition, OGD/R exposure led to increase the levels of intracellular reactive oxygen species (ROS), which were
significantly suppressed by tanshinone IIA treatment. Furthermore, tanshinone IIA treatment inhibited elevations in MMP and autophagy following exposure to OGD/R. Additionally, OGD/R promoted cell death with concomitant inhibiting phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/
mammalian target of Rapamycin (mTOR) pathway, which was reversed by tanshinone IIA. Conclusion: These results suggest that the tanshinone IIA protects against OGD/R-mediated cell death in HT-22 cells, in part, due to activating PI3K/Akt/mTOR pathway.
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International Journal of Nanomedicine
·
2017
·
IF 4.3
Fang Shan,YuJuan Liu,Haiying Jiang,Fei Tong
Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly( l -lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1 H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yang Yu,Peng Li,Guohai Zheng,Liwei He,Dandan Ge,Zhehui Weng
A magnetic matrix solid phase dispersion (MSPD) method based on magnetic ZIF-8 (SDS@MZIF-8) and a deep eutectic solvent was developed and used to the extraction of crystal violet and rhodamine B in lake sediments. SDS@MZIF-8 was prepared and used as the extraction sorbent and dispersant in MSPD. The magnetic property of MZIF-8 facilitated the recovery of the extraction sorbent after the blending step. Hydrophilic deep eutectic solvents were prepared and used as efficient green dispersing solvents in MSPD. The use of magnetic sorbent in MSPD is simple and fast, eliminating some additional steps commonly used in the classical MSPD and shortening the extraction time. The parameters influencing the extraction efficiency were investigated and optimized. Under the optimized conditions, the intra- and inter-day relative standard deviations were less than 4.9 %. The linearity ranged from 2-400 µg g −1 with coefficients of determination higher than 0.9943. The detection and quantification limits of RB and CV varied between 0.3–0.5 μg g −1 and 1.0–1.8 μg g −1 , respectively. Finally, the developed method was applied to the analysis of RB and CV in the sediment samples. The recoveries ranged from 93.2 to 107.3 % and the relative standard deviations ranged from 0.8 to 4.9 %. The results suggest a high potential use of the proposed method to determine the organic pollutants in solid samples. Compared with the conventional MSPD extraction technique, the developed method considerably reduced the sample and solvent consumption, offering important environmental benefits.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Liwei Gao,Dingfeng Wang,Yihao Zhang,Linfan Wang,Ting Zhang,Guoqiang Guo,Da Chen
The extensive use of methyl parathion (MP) has resulted in both immediate and long-term health hazards to humans. Therefore, developing a practical and efficient on-site detection system for MP is essential. In this study, o -phenylenediamine and 4-vinylphenylboronic acid are used as precursors for synthesis of triple excitation boron and nitrogen co-doped carbon dots (B, N-CDs) utilizing a one-step solvothermal process. Significantly, MP hydrolyzes to p-nitrophenol in a highly alkaline environment, and the absorption bands of this compound partially coincide with the excitation spectra of B, N-CDs. Three excitation peaks show different responses to MP: the two shorter-wavelength excitation peaks are significantly quenched, while the longer-wavelength excitation shows minimal quenching. This phenomenon facilitates the development of a ratiometric fluorescence sensor utilizing the internal filter effect (IFE) targeting sensitive MP detection. The sensor has a low limit of detection (LOD) of 74 nM and a wide linear response spanning 0.074 to 100 μM, indicating its potential applicability in food monitoring. Moreover, a visual detection platform incorporating lateral flow assay strips technology is employed for MP analysis in fruits and vegetables. The system maintains relative standard deviations (RSDs) below 2.32 % while achieving satisfactory recovery rates ranging from 98.1 % to 103.3 %. Thus, this study offers a novel effective strategy for on-site MP detection in the food-related environment.
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ACS Applied Polymer Materials
·
2025
·
IF 4.7
Qiao Fan,Kai Zhang,Yufei Liu,Chong Chen,Lanlan Wei,Yucheng Yin,Tingting Luo,Shigui Peng,Min He,Jie Yu
This work demonstrates a molecular engineering strategy to optimize the capacitive performance of PEDOT:PSS by precisely controlling the structure of the amphiphilic block copolymer PSS-b-PAPEG (poly(styrenesulfonate)-block-poly(allyloxy polyethylene glycol)). Five copolymers with PSS:PAPEG ratios of 2:1, 1.5:1, 1:1, 1:1.5, and 1:2 were synthesized via controlled polymerization and integrated into a PEDOT (poly(3,4-ethylenedioxythiophene)) matrix. Electrochemical testing revealed that the 1:1 ratio composite exhibited a maximum specific capacitance of 32.1 F/g at 1 A/g, attributed to the synergistic effects of microphase-separated dual continuous networks and porous structures. Multiscale characterization (XPS, SEM, TEM, AFM, BET, and contact angle measurements) revealed that the 1:1 ratio forms optimal conductive pathways, interfacial doping, and ionic diffusion channels. This study establishes that block ratio engineering is an effective method for balancing conductivity and ionic pathways in conductive polymer-based supercapacitor electrode materials, providing a theoretical basis for high-performance energy storage materials.
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Journal of Materials Chemistry A
·
2014
·Vol. 3
·期 7
·3777-3784
·
IF 0
Guojian Tian,Shengyun Cai,Xin Li,Hans Ågren,Qiaochun Wang,Jinhai Huang,Jianhua Su
Two new D–A–π–A configuration metal-free organic sensitizers (T1–T2) based on 5,7-dihexyl-6,12-diphenyl-5,7-dihydroindolo[2,3-b]carbazole and a DPP unit with a bulky branched alkyl chain have been synthesized for dye-sensitized solar cells. Due to a bulky branched alkyl chain being attached to the donor and DPP unit, both compounds obtain high Voc values. Under standard global AM 1.5 solar light conditions, the T1 based-device gives a high conversion efficiency of 8.24% with a Jsc of 15.72 mA cm−2, a Voc of 0.74 V and a FF of 0.71. These excellent performances make the donor dihydroindolo[2,3-b] carbazole and DPP unit promising candidates for further application in DSCs.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Yizhong Shen,Shaopu Liu,Ling Kong,Dan Li,Youqiu He
Thioglycolic acid-capped (TGA-capped) CdTe/CdS quantum dots (QDs) were prepared in the water phase. The interaction of TGA-CdTe/CdS QDs with polymyxin B sulfate (POBS) was investigated by ultraviolet–visible (UV–vis) absorption, fluorescence (FL), resonance Rayleigh scattering (RRS) spectroscopy and the measurement of fluorescence lifetime . Under optimum conditions, not only the UV–vis absorption and fluorescence spectra were changed, but also the RRS intensity was greatly enhanced. These phenomena offered useful techniques for the determination of POBS with fluorescence quenching and RRS methods . The linear ranges and detection limits of POBS were 0.09–5.25 μg mL −1 and 27.5 ng mL −1 for the fluorescence quenching technique, 0.02–6.0 μg mL −1 and 6.36 ng mL −1 for the RRS technique. Among them, the RRS approach obtained the higher sensitivity. Accordingly, a novel rapid, convenient, and highly sensitive RRS method for the determination of POBS was proposed and applied to detect POBS in pharmaceutical preparations with satisfactory results. Furthermore, the suitable reaction conditions, affecting factors as well as the influence of coexisting substances were studied. Some possible reaction mechanisms were also discussed.
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RSC Advances
·
2025
·Vol. 15
·期 58
·49875-49888
·
IF 4.6
Yangyi Cai,Xiaocan Liu,Weiye Yang,Hongyan Peng,Lijian Meng,Shihua Zhao
Persistent organic pollutants pose significant long-term risks to environmental and human health due to their carcinogenic, mutagenic, and teratogenic properties. This study focuses on the efficient activation of peroxymonosulfate (PMS) through the rational design of a cobalt-based metal–organic framework (ZIF-67) catalyst, enabling rapid degradation of high-concentration dye pollutants. By optimizing the molar ratio of 2-methylimidazole (2-MeIM) to Co(NO3)2·6H2O and employing a hydrothermal synthesis method, we fabricated ZIF-67 (4 : 1) with high crystallinity, large specific surface area (1656.68 m2 g−1), and stable degradation performance. The ZIF-67 (4 : 1)/PMS system achieved complete degradation of 20 mg L−1 and 100 mg per L Rhodamine B (RhB) within 60 seconds and 6 minutes, respectively, surpassing recent reported efficiencies. The system also maintained high activity over a broad pH range (3–9). Radical quenching experiments and electron paramagnetic resonance (EPR) analysis identified sulfate radicals (˙SO4−4) and singlet oxygen (1O2) as the dominant reactive species. Furthermore, the catalyst exhibited excellent recyclability with no significant loss in activity after five consecutive cycles. This work provides a facile and scalable strategy for preparing highly active cobalt-based catalysts, demonstrating great potential for practical applications in industrial wastewater treatment and environmental remediation.
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