JOURNAL OF ALLOYS AND COMPOUNDS
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2025
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IF 6.3
Junzhou Men,Yi Zheng,Chen Luo,Hui Xian,Yuxia Zhang,Yating Wang,Tao Jiang
Defect engineering is crucial for optimizing the band structures and charge separation of photocatalysts. Herein, the quantity and distribution (core/shell) of organic ligand defects in leaf-shaped zeolite imidazole framework (ZIF-L) composites were modulated via a multi-step method. VZ-12@ZIF-L (with 12 % 1-vinylimidazole in the core) exhibited the highest carrier separation efficiency and the strongest driving force for the hydrogen evolution reaction (HER), achieving a hydrogen evolution rate of 443.19 μmol·h⁻¹ ·g⁻¹ . This rate is 1.81 times that of VZ-25@ZIF-L (with a high defect quantity) and 1.40 times that of ZIF-L@VZ-12 (with defects in the shell). Mechanistically, core defects facilitated the transport of photogenerated carriers, while shell defects narrowed the band gap. Notably, the impact of carrier transport capacity on photocatalytic activity was more significant than that of light absorption. This study provides new insights for the design of efficient defect-engineered metal-organic frameworks (MOFs).
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Journal of Macromolecular Science Part B-Physics
·
2020
·
IF 0.911
Hailin Xie,Zhihua Zhou,Wenjuan Liu,Yanmin Zhao,Tianlong Huang,Ping Chen,Ziwei Zhou,Dan Wang,Jianglong Duan,Jianjun Fang
Polymeric/ceramic composite scaffolds that are biocompatible and biodegradable are widely used for tissue engineering applications. In this work a series of poly(L-lactide-co-glycolide-co-ε-caprolactone)/nano-biaoactive glass-nano-β-tricalcium phosphate composite scaffolds were successfully fabricated and the influences of the inorganic content and freezing temperature on the physical properties were studied. The composite scaffolds with various inorganic contents showed an interconnected pore structure with irregular shapes. The composite scaffolds had a porosity that was reduced with increasing inorganic content and decreasing freezing temperature. The incorporation of inorganic fillers and decreasing freezing temperature improved the mechanical properties of the hybrid scaffolds. By appropriate control of these two factors (10.0 wt% content of NBAG and β-TCP with freezing at −30 °C) a suitable composite scaffold was prepared as a potential bone tissue engineering implant.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
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2025
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IF 6.8
Jiayi Song,Maomao Ma,Yijian Wu,Yan Hu,Xinyi Wu,Peipei Guan,Bin Zeng
Green mold, caused by Penicillium digitatum , is one of the most destructive postharvest diseases of citrus fruits, leading to significant rot and economic losses. In this study, the antifungal activity and underlying mechanism of the novel antimicrobial peptide (AMP) N1–6L7A against P. digitatum were investigated. Additionally, the potential application of N1–6L7A for controlling postharvest green mold in Citrus sinensis (L.) Osbeck was examined. N1–6L7A exhibited potential antifungal efficacy by inhibiting both mycelial growth and spore germination of P. digitatum . Furthermore, it demonstrated excellent thermal stability, pH tolerance, and biosafety. The results of fluorescence microscopy assay, cell component leakage, and microscopic observations revealed that N1–6L7A severely damaged the fungal membrane of P. digitatum , leading to the release of intracellular nucleic acids and proteins. N1–6L7A treatment also induced excessive intracellular reactive oxygen species (ROS) production, collapse of mitochondrial membrane potential, and decreased activities of malate dehydrogenase (MDH) and ATPase. Gel retardation assay indicated that P. digitatum -DNA was a target of N1–6L7A. Furthermore, transcriptomic analysis suggested that N1–6L7A might inhibit the growth of P. digitatum by blocking the expression of genes related to the ribosome, amino acid metabolism, and pyrimidine metabolism. Meanwhile, the peptide potentially promoted the expression of genes involved in steroid biosynthesis. Notably, in vivo assays confirmed that N1–6L7A effectively controlled P. digitatum infection in citrus fruits and enhanced host resistance by activating defense-related enzymes. Collectively, our results suggested that N1–6L7A could be used as an alternative fungicide to prevent green mold in citrus fruits.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
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2026
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IF 5
Xiaoyang Li,Xiaodan Wang,Yan Ming,Rongtao Li,Yun Yang,Jing Wang,Baiyu Chang,Qing Zhang,Jingling Tang,Jiaxin Liu,Hongyu Ji,Congcong Lin
Multifunctional microneedles MR-NPs-MNs was developed for chronic wound treatment, loading mupirocin and L-arginine into PLGA nanoparticles. • The MR-NPs-MNs penetrate the stratum corneum, facilitating deep drug delivery and a synergistic antibacterial effect with mupirocin. • Co-encapsulation of mupirocin and L-arginine in MR-NPs synergizes immediate antibacterial action with wound microenvironment remodeling. • MR-NP s-MNs simultaneously address infection, inflammation and tissue repair to accelerate wound healing. Chronic wounds pose notable clinical challenges due to persistent infection, inflammation, and impaired tissue regeneration. We designed a multi-functional microneedle patch (MR-NPs-MNs) comprising chitosan-based microneedles encapsulating PLGA nanoparticles co-loaded with mupirocin and L-arginine. As a key component, L-arginine significantly enhances anti-inflammatory effects by reducing pro-inflammatory cytokines and effectively promotes tissue repair by regulating oxidative stress and facilitating angiogenesis. The PLGA nanoparticles MR-NPs exhibited stable physical properties (181.7 ± 5.875 nm particle size, 22.4 ± 0.529 mV zeta potential) and were incorporated into microneedles capable of withstanding 40.38 N compression. Notably, MR-NPs-MNs outperformed conventional mupirocin ointment, achieving a 99.82 ± 0.15% antibacterial rate (vs. 90.69 ± 1.11% for the ointment), superior biofilm inhibition (96.82 ± 0.83% and 77.8 ± 5.32%), and enhanced transdermal drug delivery. In vitro, MR-NPs-MNs increased NO production, reduced TNF-α, IL-6 and ROS levels, and promoted wound healing. In diabetic wound models, MR-NPs-MNs increased the healing rate by 1.54-fold, significantly reduced bacterial counts ( P < 0.01) by day 9, and improved key healing parameters including collagen matrix reorganization, robust neovascularization, and functional tissue formation. With its biocompatible components, this system provides a potential local therapeutic platform for infected diabetic wound treatment. Download: Download high-res image (153KB) Download: Download full-size image
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CHEMICAL ENGINEERING JOURNAL
·
2026
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IF 12.5
Huanzhong Zeng,Rui Liu,Yuanyou Peng,Jiyi Sun,Junlong Chen,Jinling Li,Jianzhou Niu,Fuliang Zhu,Fen Ran
Carbon-based composites with nano dispersed transition metal sulfides (TMS)/transition metal phosphides (TMP) are successfully prepared using a bio-assisted strategy, where non-ferrous metals in leaves are converted to TMS and those in stems are converted to TMP. Carbon films obtained from the conversion of naturalized bioreactor (plant cells) membranes not only successfully achieve the nano-materialization of TMS/TMP, but also ensure good conductivity and can effectively accommodate the volume changes during cycling and maintain structural integrity. Besides, nonferrous metals will also optimize the dense structure of leaf-derived carbon during calcination, exposing more active sites and ion transport channels. Benefiting from these, the as-prepared carbon-based cobalt sulfide composite (CdL Co) exhibits excellent electrochemical performance as an anode for lithium-ion batteries (LIBs), including high reversible capacity, outstanding cycling stability, and good rate performance. Especially after 1, 000 cycles under 1 A g −1 , a high reversible capacity of 441 mAh g −1 can still be maintained. Besides, the dual-conversion achieved by this bio-assisted strategy can also be used to explore the preparation of nanocomposites for other applications, with universality.
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Journal of Functional Foods
·
2026
·
IF 3.9
Ying Zhang,Xue Zhang,Qiu Zhong,Mingyue Yu,Qianfei Wei,Caiping Xu,Yuecheng Liu,Yi Zhao,Yi Wu,Xin Wu,Yanyun Che
Ulcerative colitis (UC) is a chronic inflammatory bowel disease that is becoming increasingly prevalent worldwide. It has a complex, multifactorial pathogenesis and limited therapeutic efficacy. Emerging evidence indicates that Phyllanthus emblica polyphenols (PEP) exert protective effects against UC. However, its precise molecular mechanisms have yet to be fully elucidated. In this study, 71 polyphenolic compounds were identified from PEP using UPLC-Q-Exactive Orbitrap/MS, including 8 flavonoids, 27 phenolic acids, and 2 lignans. The symptoms of UC in mice were significantly reduced by PEP when induced with dextran sulfate sodium (DSS). Histological and biochemical analyses revealed that PEP ameliorated mucosal damage, restored goblet cell numbers, and enhanced the expression of tight junction (TJ) proteins, indicating improved intestinal barrier integrity. This restoration of barrier function was associated with the attenuation of endoplasmic reticulum (ER) stress, evidenced by the downregulation of the ATF4/eIF2α/CHOP signaling pathway, and a concurrent reduction in intestinal epithelial cell apoptosis involving the modulation of the Bax/Bcl-2 balance and Caspase 3 expression. In parallel, gut microbiota analysis showed that PEP restored microbial community structure, increasing the relative abundance in a higher Firmicutes/Bacteroidetes ratio. Furthermore, PEP enhanced short-chain fatty acid (SCFA) production, particularly acetic, propionic, isobutyric, and isovaleric acids, which were positively correlated with beneficial bacterial taxa. Collectively, these findings demonstrate that PEP exerts therapeutic effects against UC by modulating the interplay between intestinal barrier integrity, ER stress, the apoptotic pathway, and the gut microbiota-SCFA metabolism axis.
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Food Chemistry-X
·
2026
·
IF 9.2
Rong Ma,Chen Chen,Zihang Shi,Yangyang Hu,Yangying Sun,Qiang Xia,Changyu Zhou,Daodong Pan
Although pulsed electric field (PEF) and L-arginine alone can improve meat tenderness, their synergistic molecular mechanism remains unclear. This study first explored the tenderization mechanism of combined PEF and L-arginine (LP) in goose meat. Results showed that LP reduced goose meat shear force by 51.30%, with effects significantly superior to either treatment alone. Hematoxylin-eosin staining revealed that LP enlarged intermuscular spaces. Infrared spectroscopy indicated that LP induced a conformational shift from α-helix to β-sheet in myofibrillar proteins (MPs). Fluorescence spectroscopy showed a significantly increased fluorescence intensity, confirming structural unfolding of MPs. Enzyme activity assays showed that LP elevated calpain activity by 64.33%. SDS-PAGE further confirmed the accelerated degradation of troponin-T. Molecular dynamics simulations revealed that PEF primarily drives the restructuring of the enzyme-substrate binding interface, while L-arginine stabilizes the process by maintaining the hydrogen bond network. This study provides a theoretical basis for developing efficient and controllable meat tenderization technologies.
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PLANT CELL TISSUE AND ORGAN CULTURE
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2026
·Vol. 165
·期 1
·12
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IF 2.8
Erst Anna A.,Kotsupiy Olga V.,Tomoshevich Maria A.,Banaev Evgeny V.
For the first time, a mixoploid plants and callus cultures of Rhodiola rosea L. were established. Flow cytometry analysis revealed that seed treatment with 1000 µM colchicine for 24 hours induced mixoploidy in 30% of the samples, while the remaining 70% remained diploid. The combination of 5 µM thidiazuron and 5 µM α-naphthaleneacetic acid resulted in a 100% callus induction rate from cotyledonary leaves and supported callus growth up to 50 g/L dry weight. The phenylpropanoids identified in the rhizomes of in vitro plants (control, 2n) were rosavin, rosin, unidentified phenylpropanoids (PP1, PP2, and PP3), and cinnamyl alcohol. In the mixoploid calluses, the same phenylpropanoids were detected, with the exception of PP1. In the diploid calluses, no phenylpropanoids were detectable within the sensitivity limits of the analytical method used. ANOVA confirmed the significant influence of both UV exposure and cinnamyl alcohol concentration on phenylpropanoid accumulation in mixoploid calluses, with levels reaching up to 484 mg/100 g dry weight. Rosin was identified as the major phenylpropanoid in mixoploid calluses under standard conditions. However, after 72 hours of UV irradiation and cinnamyl alcohol supplementation, PP3 became the dominant compound. This study demonstrates that combining polyploidization, biotransformation, and elicitation is a promising alternative strategy for enhancing metabolite production in Rhodiola rosea cell cultures. Moreover, the targeted application of these stress factors can serve as a tool for ‘metabolic engineering’ in its callus culture. This approach may facilitate the sequential scale-up of metabolite biosynthesis in bioreactors to meet industrial demands.
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FOOD CHEMISTRY
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2026
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IF 10.4
Lin Zhang,Ruifen Zhang,Mingwei Zhang,Zhenlin Xu,Fei Huang,Xuchao Jia,Lihong Dong,Zhiming Wang,Mei Deng
A debittering method by multi-enzyme coupled hydrolysis for Shatianyu ( Citrus grandis L. Osbeck) whole fruit powder (SWFP) was established. Shatianyu whole fruit homogenate (SWFH) was hydrolyzed by pectinase and cellulase (1:1, w /w, pH 4.5, 50 °C for 2 h). The mixture was then hydrolyzed by naringinase under the optimal conditions: naringinase addition amount of 0.38% ( w /w FW), hydrolysis time for 3.0 h, and hydrolysis temperature at 50 °C. Subsequently, the hydrolyzed products were freeze dried to obtain PCNE-SWFP. Compared with SWFP, the major bitter flavonoids including naringin, melitidin and cigranoside B in PCNE-SWFP were degraded by 40.1%, 37.9% and 49.7%, respectively, and bitterness intensity was reduced by 68.7%. Notably, PCNE-SWFP still showed high total flavonoid content (18.2 mg CE/g DW), also exhibited an increased soluble dietary fiber content, antioxidant activity, as well as water adsorption, water swelling and oil adsorption capacity compared with SWFP.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Hongbo Li,Chen Li,Geng Long,Tian Su,Shuhua Zhang,Yang Zou,Xiaohong Chen,Hongjuan Li,Jinghua Yu
HA-SH-L-P is a novel redox-responsive hydrogel delivery system designed to enhance probiotic colonization, survival, and targeted delivery in the gastrointestinal tract through the synergistic interaction between l -glutamine and probiotics. This interaction not only improves probiotic viability but also modulates the immune microenvironment and strengthens intestinal barrier function. Using Limosilactobacillus fermentum CECT5716 as a model strain, the hydrogel demonstrated stable mechanical properties and responded to hydrogen sulfide (H₂S) in the intestinal microenvironment, enabling rapid probiotic release. In vitro, HA-SH-L-P significantly increased probiotic survival (by 3.56 log CFU/mL compared to free strains). In vivo, HA-SH-L-P modulated immune responses, maintained intestinal barrier integrity, and stabilized the gut microbiota. These results highlight the exceptional performance of HA-SH-L-P as a probiotic delivery system, offering a new approach for the treatment of related diseases.
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JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 4
·e71037
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IF 4.1
Xiaoliang Zhao,Haidong Wen,Yuanyuan Ma,Lixia Xiong,Yuxin Wang,Cunjin Wang,Jing Zhang,Pengcheng Shao,Yuhui Zhao,Ji Zhang,Weijie Zhang
Olive leaves, a major by-product of olive oil production, are an underutilized source of bioactive polysaccharides (olive leaves polysaccharide [OLP]). This study optimized the ultrasonic-assisted extraction (UAE) of OLP using response surface methodology, maximizing yield (4.28%) under conditions of a 1:30 g/mL solid–liquid ratio, 600 W power, and 3h extraction. The extracted polysaccharides (U-OLP) were chemically modified via phosphorylation, sulfation, and selenation. Structural analyses (Fourier transform infrared, UV, nuclear magnetic resonance) confirmed the successful introduction of functional groups. Monosaccharide composition analysis showed that both native and modified U-OLP contained the same eight monosaccharides (Man, GlcN, Rha, GlcA, GalA, Glc, Gal, and Ara), albeit in different proportions. Bioactivity assessments revealed that all modified derivatives, especially the sulfated form (U-OLP-S), exhibited significantly enhanced antioxidant (scavenging 1,1-diphenyl-2-picryl-hydrazyl, hydroxyl, and superoxide radicals) and hypoglycemic activities (inhibiting α-glucosidase and α-amylase) compared to the native U-OLP. Furthermore, in vitro cell assays demonstrated that U-OLP and its derivatives inhibited HepG2 cell proliferation and stimulated proliferation, phagocytosis, and nitric oxide release in RAW264.7 macrophages, with U-OLP-S showing the most potent immunomodulatory effects. The findings confirm that chemical modification, particularly sulfation, is a highly effective strategy for enhancing the bioactivity of olive leaf polysaccharides, broadening their application potential in functional foods and pharmaceuticals.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Chun-Pu Mao,Jiu-Ting Tan,Kai Wang,Fei-Fei Cai,Hong-Bin Dai
Herein, hepatocellular carcinoma (HCC) and acute kidney injury (AKI) were accomplished using α - l -fucosidase (AFU)-responsive fluorescent probe, HD-AFU , which incorporates the canonical AFU recognition moiety and a structurally optimized hemicyanine-thioxanthene signaling unit. In comparison with recently reported probes, HD-AFU demonstrated robust and reliable performance in monitoring AFU activity across HCC and AKI models. This probe enabled mitochondrial-targeted intracellular imaging to track AFU dynamics during progression and therapeutic intervention. In the orthotopic transplanted tumor mouse model, HD-AFU exhibited consistent capabilities for visualizing HCC status. To validate the accuracy of the probe in detecting AFU in AKI, we established four AKI models, including cisplatin-induced, gentamicin-induced, hyperuricemic nephropathy, and diabetic nephropathy models. The accurate establishment of these four models holds significant importance for subsequent fluorescence imaging studies. Additionally, we employed losartan and metformin to treat hyperuricemic nephropathy and diabetic nephropathy, respectively, and utilized the probe to achieve the screening and validation of therapeutic drugs. Furthermore, in a long-term induced HCC and AKI model validated by serum indices and histopathological assessment, HD-AFU successfully visualized the diagnostic and therapy process. These presented herein offer valuable insights for the development of effective diagnostic strategies.
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