JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Jiajun Wu,Xianfeng Lin,Jin Li,Ziyu Lv,Nuo Duan,Zhouping Wang,Shijia Wu
Acrylamide (AA) is a heat-processed potent food carcinogen that is widely used in industry, posing a significant risk to human health. Therefore, it is necessary to investigate the toxic effects and mechanism of AA. miR-21 is a representative biomarker during AA-induced carcinogenesis. Here, dual-color aggregation-induced emission nanoparticles (AIENPs) were developed for the detection and simultaneous imaging of AA and miR-21. AIENPs were synthesized by combining aggregation-induced emission (AIE) dyes and a poly (styrene-co-maleic anhydride) (PSMA) amphiphilic polymer modified with hairpin DNA. Upon AA intervention and aptamer recognition, cDNA was dissociated, leading to miR-21 overexpression and initiating the catalytic hairpin assembly cycle. Consequently, fluorescence quenching was observed due to FRET between AIENPs and labeled quenchers. The relative fluorescence intensities of dual-color AIENPs displayed good linear relationships with logarithmic AA and miR-21 concentrations. Moreover, there was a gradual decrease in dual-color AIENP fluorescence as the HepG2 cell concentration of AA (0–500 μM) and stimulation time (0–12 h) increased, making it possible to simultaneously image AA and AA-induced miR-21. The findings of this work are valuable for revealing the cytotoxic mechanism of AA.
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Polymers
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2023
·Vol. 15
·期 20
·4165
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IF 5
Haining Zhuang,Huayue Dong,Xiaowei Zhang,Tao Feng
In this study, the digestion and fermentation properties of the bioactive water-soluble polysaccharide (HEP-W), and alkali-soluble polysaccharide (HEP-A) fromHericium erinaceusand the impact on the human colonic microbiota were determined using simulated saliva–gastrointestinal digestion and human fecal fermentation models in vitro. The basic physicochemical properties of HEP-W and HEP-A were determined at the same time. The results showed that the in vitro simulated digestion had almost no effect on the physicochemical properties of HEP-W and HEP-A, indicating that HEP-W and HEP-A were partially degraded. During fermentation, HEP-W and HEP-A increased the relative abundance of the dominant butyric acid-producing genera, the microbial community structure was significantly regulated, the gas production and short-chain fatty acid production in the fermentation broth were significantly increased, and the pH of the fermentation broth was reduced. There were structural and other differences in HEP-W and HEP-A due to different extraction methods, which resulted in different results. These results suggest that HEP-W and HEP-A may be potential gut microbial manipulators to promote gut health by promoting the production of beneficial metabolites by intestinal microorganisms using different butyric acid production pathways.Keywords:Hericium erinaceuspolysaccharides;extraction method;antioxidant;in vitro fermentation;gut microbiome
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Nutrients
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2023
·Vol. 15
·期 19
·4206
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IF 5.9
Yu-Heng Mao,Minghan Wang,Yu Yuan,Jing-Kun Yan,Yanqun Peng,Guoqin Xu,Xiquan Weng
Excessive exercise without adequate rest can lead to overtraining syndrome, which manifests a series of side effects, including fatigue, gut dysbiosis, and decremental sports performance. Konjac glucomannan (KGM) is a plant polysaccharide with numerous health-improving effects, but few studies reported its effects on the gut microbiome, endurance, and strength in an overtraining model. This study assessed the effect of KGM on gut microbiome, endurance, and strength in mice with excessive exercise. Three doses of KGM (1.25, 2.50, and 5.00 mg/mL) were administrated in drinking water to mice during 42 days of a treadmill overtraining program. The results showed that excessive exercise induced a significant microbial shift compared with the control group, while a high dose (5.00 mg/mL) of KGM maintained the microbial composition. The proportion ofSutterellain feces was significantly increased in the excessive exercise group, while the moderate dose (2.50 mg/mL) of KGM dramatically increased the relative abundance ofLactobacillusand SCFA production in feces. Additionally, the moderate dose and high dose of KGM counteracted the negative effects of excessive exercise on strength or/and endurance (43.14% and 39.94% increase through a moderate dose of KGM, Bonferroni correctedp< 0.05, compared with the excessive exercise group). Therefore, it suggests that KGM could prevent overtraining and improve sports performance in animal models.Keywords:polysaccharides;prebiotics;dietary fiber;performance;microbiota
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ULTRASONICS SONOCHEMISTRY
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2023
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IF 8.4
Hongli Zhang,Xinpeng Li,Miao Kang,Zhanrong Li,Xiaowen Wang,Xu Jing,Jiajun Han
The sustainable extraction of saponins was investigated using natural deep eutectic solvents (NADESs) combined with ultrasound-assisted extraction. A novel NADES (butyric acid-urea) that was responsive to ionic strength was designed and used as the extractant. Ultrasound treatment and a catalyst ferric chloride with plant cell wall breaking function were applied to improve the extraction efficiency. Since the solubility of the NADES varied significantly with ionic strength, 95% of NADES was readily separated from the water phase after the addition of sodium chloride, while saponins remained in the water phase for easy collection. The reuse capacity of NADES, the eco-friendliness of the extraction method, and the antioxidant activity of the extract were further evaluated. NADES was continuously recovered and used to extract Polygonatum sibiricum powder: the yield of saponins did not decrease after five cycles of recovery and re-extraction. The penalty point on the “Eco-scale” suggested that the extraction method was “green” ( i.e . eco-friendly). Compared with ethanol extracts, the NADES extracts showed a higher saponin concentration and antioxidant activity. The study can contribute to the sustainable and green extraction of hydrophilic active substances in the food and pharmaceutical industries.
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APPLIED CATALYSIS A-GENERAL
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2023
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IF 5.5
Luping Zhang,Xuewei Tu,Yutong Chen,Shouxin Zhu,Can Sun,Yanjiang Song,Hui Zheng
The conversion of CO 2 to cyclic carbonate under mild conditions is an effective strategy to achieve carbon neutrality . This work reports the formation of cyclic carbonates by coupling CO 2 with epoxides using a photothermal catalyst (CoFe 2 O 4 @PDA) with added organic ligands of bimetals under atmospheric pressure, solvent-free, 70 °C and UV light irradiation. The addition of ligands substantially enhanced the photothermal catalytic performance of the catalysts as verified by various characterizations. Subsequent experimental results under optimal conditions showed excellent yields of the catalysts for the conversion of epoxides with different groups to the corresponding cyclic carbonates. The cycling results also indicate good recyclability of the catalyst. This pathway in using light as a driving reaction to fix CO 2 for the synthesis of cyclic carbonate provides a new idea for subsequent photothermal catalytic fixation of CO 2 .
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FOOD CHEMISTRY
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2023
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IF 8.8
Baoxin Cheng,Xiuhua Xia,Zhiqiang Han,Hang Yu,Yufei Xie,Yahui Guo,Weirong Yao,He Qian,Yuliang Cheng
Acrylamide, as a Class 2A carcinogen, poses serious threats to human health. To achieve rapid and accurate determination of acrylamide in food, a ratiometric fluorescent “off–on” sensor was designed by incorporating red-emitting copper nanoclusters and glutathione . Copper nanoclusters with bimodal emission at 395 nm and 650 nm (excited at 310 nm) were synthesized by using bovine serum albumin as the ligand and ascorbic acid as the reductant. With glutathione addition, the fluorescence intensity at 650 nm gradually decreased, while the case at 395 nm slightly increased. The quenched fluorescence at 650 nm was subsequently restored by acrylamide through thiol-ene Michael addition reaction between acrylamide and glutathione. The constructed sensor showed excellent performance towards acrylamide detection in the range of 5–300 μM with a detection limit of 1.48 μM, and was further applied to real-sample detection of acrylamide in toast and exhibited good recoveries (90.29–101.30 %), indicating potential applications of this sensor.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2023
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IF 4.3
Lina Qiao,Jing Wang,Bing Liu,Junyi Wang,Ruifang Wang,Ning Zhang,Baoguo Sun,Yuping Liu,Shuqi Wang,Jie Sun
The flavor of deep-fried green onion oil (FO) changed significantly at 155 °C, with decreased fresh onion-like aroma and green scent, and increased savory and burnt notes. A total of 144 odorants were identified in FO samples. The total content of sulfur-containing odorants was highest at 155 ℃, while the total content of target furans and aldehydes/ketones peaked at 165 ℃. UFA accounted for approximately 95 % of the non-volatile fractions in FO, mainly in the form of PUFA . The oxidation degradation of UFA at high temperatures promoted the generation of small molecule aldehydes. Odorants in FO predominantly originated from reactions between sugars and amino acids , contributing to the enticing aroma.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 3.3
Jie Xiong,Ya-ying Wang,Muhammad Yousaf,Dong-mei Liu
Summary The fermented vegetable is a long-history food, and the addition of probiotics in recent years has pushed it in a novel direction. This study explored the impact of a Direct-vat set starter (DVS), comprising Lactiplantibacillus plantarum MC14 and Leuconostoc mesenteroides , on pickled radish (PR). The addition of DVS triggered a rapid reduction in pH and titratable acidity, leading to lowered nitrite levels and increased viable count. Textural analysis showed that DVS increased the springiness and chewiness of PR by about 55.07% and 25.73%, respectively. Organic acid analysis unveiled eight compounds. The addition of probiotics did not significantly affect the antioxidant properties but was able to significantly enhance the XO inhibitory activity of PR. DVS decreased undesirable flavor compounds such as isothiocyanates and sulfides, while elevating esters, aldehydes, and acids. Our results suggested that DVS is a promising starter culture that can enhance the texture and flavor of fermented vegetables.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
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IF 4.1
Jing Cheng,Guangwen Zhang,Liu Liu,Jianming Luo,Xichun Peng
BACKGROUND Beta-glucan is widely sourced and has various physiological activities including anti-inflammation. However, the strength of the anti-inflammatory activity of β-glucans from different sources remained elusive due to the lack of rapid and effective biomarkers. Therefore, this study aimed to screen out the β-glucan with strong anti-inflammatory activity from 5 different sources and to further screen out possible biomarkers in metabolites after fermented the β-glucans by gut microorganisms. RESULTS The results showed that all five β-glucans inhibited the production of LPS-induced pro-inflammatory mediators and suppressed the mRNA expression level of TLR4/MyD88. Their anti-inflammatory mechanisms involved the inhibition of intracellular ROS production and suppression of mRNA expression of the NF-κB pathway and JNK pathway. Among them, barley β-glucan exhibited the strongest anti-inflammatory effect, followed by Ganoderma lucidum β-glucan. Enhanced anti-inflammatory activity of β-glucan after fermentation was found and may be related to the increased abundance of metabolites such as the vanillin, dihydroxyphenylacetic acid, caffeic acid, acetic acid, butyric acid, and lactic acid. They are strongly positively correlated to the abundance of beneficial bacteria such as Blautia , suggesting that the production of those metabolites may be responsible for the flourishing of the beneficial bacteria. CONCLUSION In conclusion, barley was a preferred raw material for the preparation of β-glucans with high anti-inflammatory activity. Vanillin, dihydroxyphenylacetic acid, caffeic acid, acetic acid, butyric acid, and lactic acid were the possible biomarkers that could be utilized to evaluate the anti-inflammatory effect of β-glucans. This article is protected by copyright. All rights reserved.
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Food Bioscience
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2023
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IF 5.2
Zuanyuan Huang,Mengwei Hu,Xiuyi Peng,Rui Wang,Xiaoxiao Song,Junyi Yin
Small black soybean ( Vigna Mungo L.) is a kind of medicinal and edible plant with many potential health benefits for the human body. In this study, we investigated the potential anti-gastric ulcer effect of Vigna Mungo L. polysaccharides (VMP) using an acetic acid-induced gastric ulcer model in SD rats . The impact of VMP on the levels of antioxidant factors and growth factors in gastric tissue homogenate , serum metabolites, short-chain fatty acids (SCFAs) and gut microbiota in SD rats were analyzed. The results revealed that VMP exhibited a beneficial role in facilitating the healing process of gastric ulcers, specifically by reducing the area of gastric ulcers, increasing the levels of epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF), and improving oxidative stress status. Additionally, VMP modulated 20 potential serum biomarkers associated with gastric ulcers, thereby exerting a significant impact on multiple crucial metabolic pathways. Notably, VMP notably increased the levels of SCFAs, including acetic acid, propionic acid , i-butyric acid, n-butyric acid, i-valeric acid, n-valeric acid, in the colonic contents of rats with gastric ulcers. Furthermore, VMP effectively ameliorated gut microbiota dysbiosis in gastric ulcer rats by reducing the relative abundance of Firmicutes , Lachnospiraceae_NK4A136_group , Quinella and Lactobacillus , while increasing the relative abundance of Bacteroidota , Bacteroides , Oscillospiraceae_UCG-005 , Muribaculaceae , Parasutterella and Ruminococcus . Correlation analysis showed that the relative abundance of Parasutterella and Lachnospiraceae_NK4A136_group were closely related to the changes of SCFAs and serum metabolites. These findings provide potential groundwork for the development of health products aimed at preventing and treating gastric ulcers.
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BIOMEDICINE & PHARMACOTHERAPY
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2023
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IF 7.5
Xiongjie Sun,Yu Zhang,Gang Cheng,Tianxiang Zhu,Zhigang Zhang,Lei Xiong,Haiming Hu,Hongtao Liu
Ulcerative colitis (UC) has been confirmed as a disease with a high incidence and low cure rate worldwide. In severe cases, UC can develop into colon cancer. Modern research has confirmed that berberine (BBR) can treat UC by inhibiting the expressions of inflammatory factors. However, the contribution of gut microbiota and flora metabolites in treating UC with BBR remains unclear. In this study, the ameliorative effects of BBR on gut microbiota dysbiosis and flora metabolites were investigated in a dextran sodium sulfate (DSS)-induced UC rodent model. We found that BBR significantly improved the pathological phenotype, attenuated intestinal barrier disruption, and mitigated colonic inflammation in DSS mice. By 16 S rDNA sequencing, BBR alleviated gut microbiota dysbiosis in UC mice. Moreover, the gut microbiota depletion experiment confirmed that the therapeutic effect of BBR was inextricably correlated with the gut microbiota. Besides, the flora metabolites ( e.g. , short-chain fatty acids, bile acids, and 5-hydroxytryptamine) were studied using HPLC-MS. The results suggested that BBR ameliorated the bile acid imbalance induced by DSS in the liver and gut. Furthermore, BBR treatment repaired gut barrier damage. The above results revealed that BBR alleviated DSS-induced UC in mice by restoring the disturbed gut microbiota, elevating unconjugated and secondary bile acids in the gastrointestinal tract, and activating the FXR and TGR5 signal pathway. This study provides novel insights into the mechanism of BBR in treating UC.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2023
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IF 3.4
Taixiang Gao,Hongxiong Zhang,Qinqing Li,Feng Zhao,Nan Wang,Wenbin He,Junlong Zhang,Rui Wang
Fuzi decoction (FZD) is clinically used to treat chronic heart failure (CHF) in China, but the mechanism underlying FZD treatment in CHF remains unclear. Here, we investigated the potential mechanism underlying FZD treatment of CHF in rats. First, the compounds in FZD-containing serum of rats were identified, and 16 S rRNA sequencing and GC–MS-based untargeted metabolomics analysis were then performed. The levels of fecal short-chain fatty acids (SCFAs) were determined and compared, and fecal microbiota transplantation (FMT) was used to verify the role of the gut microbiota . Our results identified 27 in FD-containing serum. FZD increased the Firmicutes -to- Bacteroidetes ratio and the Lactobacillus abundance and affected the β diversity of the gut microbiota in rats with CHF. Differential species analysis showed that Lactobacillus and Prevotella were biomarkers of FZD treatment of CHF. Untargeted metabolomics analysis revealed that FZD affected valine , leucine and isoleucine biosynthesis; galactose metabolism; and aminoacyl-tRNA biosynthesis in rats with CHF. Furthermore, FZD significantly increased the acetic acid, propionic acid , butyric acid and isopentanoic acid levels in the feces of rats with CHF. Correlation analysis showed that the butyric acid and Lactobacillus levels had the strongest correlation in the control, sham and high-dose FZD (HFZD) groups, and many microbiota components were closely related to differentially abundant metabolites. FMT revealed that the fecal microbiota obtained from the HFZD group changed the heart rate; the brain natriuretic peptide (BNP), acetic acid, propionic acid, butyric acid, and metabolite levels; and the gut microbiota in rats with CHF. In summary, our study revealed that the mechanism of action of FZD in CHF treatment may be related to improvements in the gut microbiota, elevations in the SCFA content and the regulation of valine, leucine, and isoleucine biosynthesis; galactose metabolism; and other metabolic pathways.
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