ACS Nano
·
2022
·
IF 18.027
Ruijia Huang,Xiaoqing Cai,Jihui Du,Jie Lian,Ping Hui,Minxuan Gu,Feng Li,Jidong Wang,Wenwen Chen
Delayed use of appropriate antibiotics for superbugs, particularly for extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pn), has caused extensive morbidity and mortality worldwide. Therefore, rapid and on-site antimicrobial susceptibility testing (AST) is urgently required. Unfortunately, currently, no phenotypic AST can realize a sample-to-answer result within 2 h directly from a clinical sample and without using laboratory equipment or customized devices. Inspired by observing that E. coli and K. pn can rapidly catalyze H2O2, we developed a plasmonic nanosensor that responds to the proliferation of bacteria for realizing rapid AST. The results can be determined with the naked eye, digitized using a smartphone, and validated using ultraviolet–visible spectrometry. Our assay achieved superb area under the curves of 0.9752 and 1 in a receiver operating characteristic analysis directly obtained from uncultured clinical urine samples infected by E. coli and K. pn, respectively. The entire process from sample collection to analysis takes 100 min for E. coli and 85 min for K. pn detection. Our platform provides a practical approach for performing on-site AST in clinics to improve the survival of patients. It releases the burden of superbugs and avoids the abuse of antibiotics.
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PubMed
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 11
·458
·
IF 4.229
Jianhui Xiong,Yuxi Yang,Linyu Wang,Shouhui Chen,Yan Du,Yonghai Song
A series of electrochemical sensors based on metal-porous carbon nanozymes were developed for the detection of dopamine (DA), uric acid (UA) and furazolidone (FZ). The metal-porous carbon nanozymes were prepared by calcination of porous crystalline covalent-organic frameworks (COFs) loaded metal ions. By carbonizing, the COFs was transformed into carbon nanosheets (CN) and metal ions were reduced into 5–10 nm MNPs loaded on CN uniformly (CuNPs/CN, FeNPs/CN, NiNPs/CN and CoNPs/CN). These porous MNPs/CN nanozymes were used for electrochemical detection of DA, AA and FZ, showing good performance. The electrochemical sensor based on CuNPs/CN nanozymes was used to simultaneously measure DA and UA. The linear range of DA detection was 0.015 μ–140 μM, the linear range of UA detection was 0.03 μM–175 μM, and the sensitivity of DA and UA were 1.03 μA μM−1cm−2and 0.52 μA μM−1cm−2. The sensitivity of sensors based on FeNPs/CN, CoNPs/CN and NiNPs/CN nanozymes to detect DA were 1.30 μA cm−2μM−1, 1.07 μA cm−2μM−1and 0.88 μA cm−2μM−1, the linear ranges were 35 nM–200 μM, 42 nM–250 μM and 52 nM–250 μM. The sensitivity of detecting UA were 0.310 μA cm−2μM−1, 0.587 μA cm−2μM−1and 0.360 μA cm−2μM−1, the linear ranges were 145 nM–900 μM, 77 nM–700 μM and 125 nM–800 μM. Finally, CuNPs/CN was also used to construct a FZ sensor with a linear range of 61.5 nM–200 μΜ and a detection limit of 20.1 nM. The sensors also have good reproducibility and repeatability.Keywords:electrochemical sensor;covalent-organic framework;nanozymes;carbon nanosheets
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ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Shanshan Zhan,Caihong Xu,Jiandan Chen,Qi Xiao,Zhenzeng Zhou,Zhiyuan Xing,Chunchuan Gu,Zhengzhi Yin,Hongying Liu
Epinephrine (EP) is an important neurotransmitter that plays a vital role in mammalian central nervous and hormone systems. So, the monitoring of EP is significant to evaluate human health. Until now, developing new methods for detecting EP has attracted increasing interest to avoid the previous disadvantages, such as high cost, long time-consuming, and professional operation. Acupuncture is a kind of traditional Chinese treatment. Herein, stainless steel needle, an acupuncture tool, was used as an electrochemical matrix and functionalized to be a simple, effective sensor for detecting EP. The acupuncture needle showed a typical electrochemical behavior. Gold nanoparticles coordinated polydopamine-modified acupuncture needle (AuNPs/PDA/AN) was fabricated via the green route of pure electron-transfer induced reaction of electrodepositing. The covered interface exhibited synergistic response performance to EP. The electron transfer of the phenolic hydroxyl group in EP molecular was enhanced via the superficial active site, showing the notable current peak. The generated current intensity can be used as an index to reflect the corresponding concentration. Under the optimized conditions, the fabricated biosensor expressed a wide and good linear relationship from 1 μM to 1000 μM, and a detection limit of 0.26 μM, which are competitive with former relative reports. In practical application, the fabricated sensor has been effectively used to analyze EP in real human serum, and also realized the real-time detection of EP secreted by PC12 cells irritated by salbutamol. The sensor was found to perform high selectivity , stability, and reproducibility, which is promising in the future given the advantages of electrochemical sensors , including low price, fast response, and simple operation.
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ANALYST
·
2022
·Vol. 147
·期 24
·5670-5679
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IF 5.227
Ziyan Song,Siyu Chen,Qiushi He,Hu Liang,Guangyao Huang,Pan Li,Zongyao Hao,Liangbao Yang
The rapid and sensitive detection of ultra-trace marker molecules from biological samples is of great significance for the wide application of surface-enhanced Raman spectroscopy (SERS) methods in clinical diagnosis and disease monitoring. However, the cumbersome biological sample processing procedures and the poor enrichment of target analytes in hot spots hinder the practical applications of SERS methods. In this paper, we synthesized a novel floating SERS substrate by a simple one-step oxidation process, annealing and in situ chemical etching to form Ag-NPs@Cu-NW bundles on copper mesh (CM). In particular, under spontaneous bottom-up capillary action, the pressure difference at different nanogaps drives uric acid molecules to actively enter hot spots, so that the Ag-NPs@Cu-NW bundle nanostructure with the advantages of a light weight CM is capable of preventing the common coffee-ring effect and enhancing the spatial enrichment of analytes. Therefore, this SERS substrate realizes highly sensitive detection of uric acid at a level of 50 nM in pretreatment-free urine. Currently, this portable, flexible, simple, fast and cost-effective SERS substrate has great potential for early screening and clinical diagnosis of diseases in different biofluids.
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高影响因子
Materials Today Chemistry
·
2022
·
IF 7.613
X. Liu,J. Song,B. Yin,T. Jiang,H. Pang,L. Yang,S. Wu
Ascorbic acid (AA) is a critical nutrition and chemical for humans, due to its crucial role in metabolic processes and medical treatments. It is necessary to develop a simple and rapid method for real-time tracking AA level in the body. In this work, we fabricated a powerless, gas-permeable, flexible and transparent AA biosensor based on carbon micromesh electrode (CME). The AA biosensor worked without an external power supply and exhibited excellent performances, including high transmittance (>95%), wide detection range (1 μM–1 mM), low limit of detection (10 nM), good selectivity and long-term storage stability (30 days). In addition, we integrated a pH-sensing electrode to correct the AA analysis. A layer of polyaniline (PANI) film was electrodeposited on the carbon micromesh as pH-sensing electrode that had an average sensitivity of 58.1 mV/pH with a high correlation coefficient ( R 2 = 0.997) within the physiological range of human body fluids (pH 4.0–8.7). To verify the availability of the CME in practical application, the Ag/C electrode was used as reference electrode to fabricate the integrated dual-functional biosensors for artificial tears detection. The CME-based biosensors showed good sensitivity, selectivity and correlation coefficient, indicating its excellent practicability towards wearable electronics.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 19
·5917-5928
·
IF 4.478
Zuo Jiabao,Shen Yuliang,Gao Juanjuan,Song Haiou,Ye Zhiwen,Liang Ying,Zhang Shupeng
Flexible sensing is an alternative to traditional sensing and possesses good flexibility and wearability. Intrinsically conductive polymers, particularly poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT:PSS), have received significant attention due to their high mechanical flexibility and good biocompatibility. Here, we report the design of highly conductive and electrochemically active PEDOT:PSS-coated plastic substrate electrodes by combining N-doped graphene (NG) or S-doped graphene (SG) with methanesulfonic acid-treated PEDOT:PSS (denoted as NG-f-MSA-PEDOT:PSS/PET and SG-f-MSA-PEDOT:PSS/PET) by a simple drop-coating method. At room temperature, the NG-f-MSA-PEDOT:PSS/PET electrode demonstrated the lowest detection limits of 17.09, 33.84, 28.30, and 44.96 nM for paracetamol, uric acid, dopamine, and catechol (S/N = 3), respectively. The NG-f-MSA-PEDOT:PSS/PET electrode had good anti-interference ability and reproducibility without employing expensive noble metals and requiring much effort to polish the surface of traditional glass carbon electrodes. Most importantly, this film electrode could maintain a stable electrochemical response under different bending and crease states and had excellent mechanical stability and flexibility. Graphical abstract
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Jing Zhang,Zhujun Kuang,Hui Li,Shaoguang Li,Fan Xia
With the development of medical science and the extension of people's life expectancy, medical care and health monitoring in daily life have become a universal, worldwide concern. Accurate quantification of glucose is of great concern in diagnostics because it can indicate diabetes and help avoid related medical complications. Over the years, a variety of analytical methods have been used to sense glucose with good selectivity , good feasibility, sensitive response and low cost. Nevertheless, there is a lack of universal approach which can be applicable for the measurements across a variety of biological fluids (e.g., blood, sweat and urine), as the concentrations of glucose in there fluids vary significantly from low-micromolar to millimolar range. To fill this role, we proposed a glucose biosensor platform to improve the adaptability of glucose biosensor in clinical application. In our study, we coupled an electrochemical etching approach with the manipulation of nanostructured surfaces of gold electrodes to achieve arbitrarily tunable detection range for glucose measurements, with the detection range in blood sample (20 ∼ 1000 μM), sweat (6 ∼ 1000 μM), and urine (1.6 ∼ 100 μM), respectively.
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ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Rufeng Zhu,Yuedan Wang,Yang Tao,Yao Wang,Yuanli Chen,Mufang Li,Qiongzhen Liu,Liyan Yang,Dong Wang
A novel composites conductive fiber based on Poly(diallyldimethylammonium chloride) and multi-walled carbon nanotubes/Poly(3, 4-ethylenedioxythiophene)-Poly(styrenesulfonate) has been prepared using layer-by-layer assembly (LBL) technology. The characterization results showed the composites film was successful prepared on the cotton fiber and had flexibility, conductivity and washability. The fiber-based organic electrochemical transistors (FECTs) have been assembled by the LBL composite fiber, which exhibit an on/off ratio of 5.12*10 2 ±17.99, a peak transconductance value of 8.7 mS, a switching response time of 1.2 s, and excellent cycle stability. Bending and repetitive hand-washing cycles of fibers have no significant influence on the electrical properties of FECTs. Moreover, FECTs maintain stable electrical properties within a week. The gate electrode of the FECT was modified with 3-aminophenylboronic acid to prepare a sialic acid (SA) biosensor. The SA biosensor based on FECTs presented a sensitivity of 57.66 mV per decade, a good linear response in a wide concentration range of 1 nM-1 mM, a linear coefficient R 2 =0.9848. This biosensor can be successful used to detect the SA content in saliva. Most importantly, the sensor can be integrated into fabrics with no significantly change in the device performance before and after weaving, providing an efficient detection platform for wearable healthcare application.
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-13
·
IF 6.408
Chen Jing,Li Shuying,Chen Ying,Yang Jiao,Dong Jianbin
Adenine (A) and guanine (G) are mainly found in deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) and play a crucial role in genetic information transfer and protein synthesis. In this study, NH 2 -MIL-53(Fe)/CS/MXene nanocomposites were prepared for detecting guanine and adenine. With high specific surface area, excellent water dispersion, and numerous active sites, MXene (transition metal carbides, nitrides, and carbonitrides) provides a good platform for loading primitive metal-organic frameworks (MOFs). At the same time, the problem of poor conductivity and dispersion of MOFs is solved. The electrochemical catalytic oxidation of adenine and guanine of NH 2 -MIL-53 (Fe)/CS/MXene nanocomposites was carried out by differential pulse voltammetry (DPV). Operating voltage of DPV: 0.7–0.9 V (vs. Ag/AgCl) for G, 1.0–1.2 V (vs. Ag/AgCl) for A, 0.8 V (vs. Ag/AgCl), and 1.1 V (vs. Ag/AgCl) for G and A. The concentration ranges for detecting A and G were 3–118 μM and 2–120 μM with detection limits of 0.57 μM and 0.17 μM (S/N = 3), respectively. The nanocomposite was used for detecting G and A in herring sperm DNA, and the content of G and A was found to be about 9 and 11 μM; the RSD values were 3.4 and 1.3%, respectively. Graphical abstract
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APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Jing Feng,Gang Lang,Tingting Li,Jing Zhang,Jun Zhao,Wei Li,Weiqing Yang,Zhenju Jiang
Coordination-driven self-assembly has emerged as a smart strategy to prepare advanced materials for various applications. Here we coat Ni(II)-tannic acid complexes on the lab-made C-Cr 2 O 3 nanoparticles by this strategy, and demonstrate that this novel coating dramatically accelerate the charge transfer and thus enhance the amperometric responses for electrochemical detection of glucose and hydrazine. The charge-transfer rate ( k s ) of the obtained C-Cr 2 O 3 @Ni-MPN is much higher than that of C-Cr 2 O 3 substrate, evaluated as 1.00 and 0.82 s −1 respectively. The physicochemical and electrochemical characterizations demonstrate that the enhanced k s of C-Cr 2 O 3 @Ni-MPN is attributed to the electronegativity and the network structure of its surface. Correspondingly, C-Cr 2 O 3 @Ni-MPN exhibits superior sensitivity (5043.3 μA mM −1 cm −2 ) and ultra-low detection limit (0.02 μM) for the glucose detection, as well as high sensitivity (918.5 μA mM −1 cm −2 ) and ultra-low detection limit (0.04 μM) for the hydrazine detection. Furthermore, C-Cr 2 O 3 @Ni-MPN exhibits the excellent selectivity, stability and reproducibility, and the powerful reliability for analyzing the plasma samples. This study constructs an appropriate hierarchical structure towards catalyzing the electrochemical oxidation of targets, which achieves the amplification of the respond current and thus an ultra-sensitive electrochemical detection for the targets.
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SENSORS AND ACTUATORS A-PHYSICAL
·
2022
·
IF 4.291
Shufang Ren,Wangyong Cui,Ying Liu,Shounian Cheng,Qingtao Wang,Runyan Feng,Zhixiang Zheng
1T phase molybdenum disulfide (MoS 2 ) possesses excellent electrical conductivity , 10 7 times higher than that of 2H phase MoS 2 . Thus, metallic 1T MoS 2 is highly desirable for electrochemical analysis and catalysis. In this study, hybrid 1T/2H MoS 2 /multi-walled carbon nanotubes (MoS 2 /MWCNTs) composite was prepared by a facile and simple hydrothermal approach. Powder X-ray diffraction, field emission scanning and transmission electron microscopes, Fourier transform infrared spectroscopy, Raman spectroscopy , cyclic voltammetry (CV), and electrochemical impedance spectroscopy were used to characterize the composites. The results show that metallic 1T phase MoS 2 was formed by the inductive effect of MWCNTs. The high conductivity and large specific surface area of hybrid 1T/2H MoS 2 /MWCNTs composite endow great catalysis activity in electrochemical analysis. Polypyrrole molecularly imprinted polymer (MIP) film imprinted with acetaminophen (AP) template was electrochemically deposited on glassy carbon electrode (GCE) modified by 1T/2H MoS 2 /MWCNTs (MoS 2 /CNTs/GCE) to construct MIP based sensor. The prepared MIP/MoS 2 /CNTs/GCE sensor shows a sensitive current response towards AP. Through the optimization of the ratio of monomer and template, CV electropolymerization cycles, CV elution cycles, incubation time, and pH, the accessible linear range of 0.01–300 μM was obtained with the limit of detection of 0.003 μM (S/N = 3) by differential pulse voltammetry . In addition, the prepared sensor shows great detecting robustness with excellent selectivity , anti-interference property, reproducibility, and stability. In the urine sample tests, the sensor shows desirable reliability with the recovery rate of 88.1–95.9%.
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高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 38
·385501
·
IF 3.953
Yanxin Liao,Qian Du,Shupei Sun,Nianfeng Shi,Guangfu Yin,Zhongbing Huang,Xiaoming Liao
Self-supported Cu2S/Cu(OH)2 composite nanorods for highly sensitive non-enzymatic glucose sensing were in situ grown on Cu foam by simple hydrothermal treatment of aligned Cu(OH)2 nanorods. The physicochemical and electrochemical properties of the as-fabricated Cu2S/Cu(OH)2 composite nanorods were characterized by scanning electron microscopy, transmission electron microscopy, x-ray diffraction, Raman spectroscope, x-ray photoelectron spectroscope, cyclic voltammetry, electrochemical impedance spectroscopy, amperometric i–t measurements. The mechanism of the composite nanorods produced on conductive substrates was also explored. The electrode exhibits a sensitivity of 9626.88 μA mM−1 cm−2 towards glucose with good anti-interference ability, indicating it a promising electrode material for the enhanced non-enzymatic glucose detection.
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