INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Lili Chen,Yuanyuan Zheng,Longbao Feng,Zonghua Liu,Rui Guo,Yuanming Zhang
The aim of this work was to examine the formation and properties of a novel polyelectrolyte complex of drug carrier system for the delivery of doxorubicin (DOX), which consists of hyaluronic acid (HA) coated hydrophobically modified chitosan (CS). Various batches of polyelectrolyte complexes with the molar ratio of deoxycholic acid (DCA) and chitosan (CS) of 0.1, 0.2, 0.3 were prepared, and were termed as CS-DCA 10 , CS-DCA 20 , and CS-DCA 30 respectively. The samples were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), Transmission electron microscopy (TEM), nuclear magnetic resonance hydrogen spectrum ( 1 H NMR) and dynamic light scattering (DLS). Particle sizes of synthesized polyelectrolyte complex nanoparticles (PCNs) were found to be in the range of 280–310 nm, larger than those of uncoated nanoparticles (~150 nm). The PCNs have large zeta potentials (about 26 mV) which make them stable and no sizes' change was determined. DOX could be easily incorporated into the PCNs with encapsulation efficiency (56%) and kept a sustained release manner without burst effect when exposed to PBS (pH 7.4) at 37 °C. Overall, these findings confirmed the potential of these PCNs for drug carrier and prolonged and sustained delivery in the bloodstream.
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JOURNAL OF SEPARATION SCIENCE
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2018
·Vol. 42
·期 4
·888-896
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IF 2.415
Tianfu Wei,Gongke Li,Zhuomin Zhang
Covalently cross-linked microporous polymers are a new class of highly cross-linked porous network materials with large surface area and potential superiority in sample pretreatment. In this work, a covalently cross-linked microporous polymer was well designed and synthesized by condensation of acylhydrazines in terephthalic dihydrazide with aldehyde groups in 1,3,5-benzenetricarboxaldehyde. The adsorption mechanism was explored and discussed based on π-π stacking interaction and steric effect. Then, a covalently cross-linked microporous polymer was employed as the adsorbent of online micro-solid-phase extraction coupled with high-performance liquid chromatography for the enrichment and analysis of trace pesticide residues in citrus fruits. The method was successfully applied to the online analysis of sugar orange and Huangdigan samples with the detection limits of 0.10–0.30 μg/kg. It was satisfactory that chlorpifos and triazophos in real sugar orange and Huangdigan samples could be actually found and quantified at concentrations of 0.20 and 0.51 mg/kg, respectively. The recoveries of sugar orange and Huangdigan samples were in the range of 70.0–103 and 74.0–119% with relative standard deviations of 0.4–9.7 and 0.5–9.2% ( n = 3), respectively. The proposed method was accurate, reliable, and convenient for the online simultaneous analysis of trace pesticide residues in citrus fruits.
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POLYCYCLIC AROMATIC COMPOUNDS
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2019
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IF 1.237
Fang Tian,Guang Guo,Keqiang Ding,Lihong Wang,Tingfeng Liu,Feng Yang
The low biological effectiveness of polycyclic aromatic hydrocarbons (PAHs) is the main limiting factor of in-situ bioremediation of PAH-contaminated soil. The purpose of the present study was to assess the effects of methyl-β-cyclodextrin (MCD) and microbial consortia on the PAHs degradation rate and microbial ecology and functional diversity and stability in soil. The results show that MCD and microorganisms significantly increased the degradation rate of pyrene and benzo[a]pyrene by 30.1% and 9.1%, respectively, (p < 0.05) after 4 weeks. The average well color development (AWCD) values and diversity indices of bioaugmentation (B) and MCD- and bacterial-assisted bioaugmentation (MB) were significantly higher than that in control (CK). The Shannon–Weaver index (H) and McIntosh index (U) in the MB treatment were significantly higher than those observed in other treatments, which indicated that its species richness and individuals were distributed evenly. The soil microbial activity and community structure were the most abundant in the MB treatment. Compared to the CK treatment, MB significantly promoted soil dehydrogenase activity (DHA) and fluorescein diacetate hydrolysis (FDAH) enzyme activity, which were increased by 96.3% and 994.3%, respectively. According to the results of principal component analysis, the physiological functions of microbial communities in the MB and B treatments were different from the other treatments. Therefore, the combination of the microorganisms with MCD can stimulate the remediation efficiency of PAHs and the physiological functions of microbial communities.
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JOURNAL OF SURFACTANTS AND DETERGENTS
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2019
·Vol. 22
·期 6
·1331-1342
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IF 1.672
Juan Wu,Ping Mei,Wu Chen,Zhong-Bao Li,Qing Tian,Qi-Xiang Mei
As a class of novel surfactants, Gemini surfactants usually exhibit fairly excellent interfacial properties in aqueous solutions on account of the unique structure. They have significant application and development potential for industrial production. However, the mixing properties of Gemini surfactants with conventional surfactants are the key to their application. The equilibrium surface tension curves of anionic/nonionic surfactant mixtures based on the sulfonate Gemini surfactant (SGS-12) were measured using the Wilhelmy Plate method. The parameters of surface adsorption, the interaction parameters between anionic and nonionic surfactants, and the thermodynamic parameters of micelle formation were calculated from the corresponding equations. In addition, the dynamic surface tension (DST) curves of anionic/nonionic surfactant mixtures were examined through bubble profile analysis, and the diffusion performance parameters were acquired from empirical formulas. The solubilization of pyrene in micelle solutions was studied using UV–vis absorption spectroscopy. The results show that the interaction parameters of all anionic/nonionic surfactants are negative, indicating that there is a synergistic effect on reducing the surface tension. For the SGS-12/OP-10, SGS-12/Tween 80, SGS-12/AEO9, and SGS-12/APG0810 mixtures, the optimum mixing ratios are 6:4, 7:3, 7:3, and 8:2, respectively. The thermodynamic data of micelles show that the formation of mixed micelles for SGS-12/APG0810 mixtures is an enthalpy-driven process. The tendency of DST curves of the SGS-12/APG0810 mixture is similar to that of SGS-12. In comparison with single-surfactant solutions, the anionic/nonionic surfactant mixtures show stronger solubilization capacity toward pyrene. Graphical
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Ping Liu,Nana Chen,Lei Yan,Fei Gao,Dongsheng Ji,Shijiao Zhang,Litao Zhang,Yuqin Li,Yuliang Xiao
The purpose of this study was to ascertain the effect of chondroitin sulphate-modified doxorubicin (Dox) nanoparticles on enhancing the tumour-targeting effect and tumour growth inhibition effect of doxorubicin both in vitro and in vivo . The chondroitin sulphate-doxorubicin conjugate and its poly(lactic-co-glycolic acid) (PLGA) nanoparticles (CS-Dox-PLGA) were successfully synthesised, and then characterized by Fourier-transform infrared spectroscopy (FTIR), proton magnetic resonance ( 1 HNMR), thermogravimetric analysis/differential scanning calorimetry (TGA/DSC), transmission electron microscope (TEM), zeta potential and laser light scattering. Taking advantage of the enhanced permeability and CD44-mediated endocytosis, CS-Dox-PLGA showed excellent capacity for penetrating the peripheral tumour barrier and into the nucleus of tumour cells. The CS-Dox-PLGA cellular uptake was improved and exhibited a significantly higher level of cytotoxicity in U251 cells. After intravenous administration, the CS-Dox-PLGA showed good pharmacokinetic properties and excellent U251-induced tumour inhibition with low cardiac toxicity. Therefore, CS-Dox-PLGA with low cardiac toxicity and good anti-tumour ability might be a better choice for Dox in clinical practice.
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CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Jing Miao,Xi-qin Yang,Zhe Gao,Qian Li,Ting-ting Meng,Jia-ying Wu,Hong Yuan,Fu-qiang Hu
DrzBC and DrzBS (10-23 DNAzyme) could block the expression of HBV e- and s- gene respectively. But the application of 10-23 DNAzyme was limited owing to the lack of appropriate delivery vehicles. Chitosan oligosaccharide-SS-Octadecylamine (CSSO), a redox-responsive nano-sized polymeric carrier, could self-aggregate and bind with DNA by electrostatic interaction at proper mass ratio. Compared with the traditional commercial carrier Lipo2000, CSSO exhibited lower cytotoxicity, efficient cellular uptake by targeting cells, and rapidly DNA released in cytoplasm after escaping from endosomes. Including the same DNA concentration, Lipo2000/(DrzBC or DrzBS) showed maximum inhibitory rate on HBeAg (47.29 ± 1.86%) and HBsAg (33.58 ± 0.72%) secretion after 48 h incubation, and then both decreased. In contrast, HBeAg secretion inhibition by CSSO/DrzBC and HBsAg secretion inhibition by CSSO/DrzBS were up to 73.86 ± 1.77% and 67.80 ± 2.51% at 48 h, and further increased to 83.83 ± 2.34% and 76.79 ± 2.18% at 72 h, respectively. CSSO is a promising redox-responsive polymeric carrier for efficient anti-Hepatitis B Virus gene therapy.
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Theranostics
·
2019
·
IF 8.063
Yanan Tan,Yun Zhu,Lijuan Wen,Xiqin Yang,Xuan Liu,Tingting Meng,Suhuan Dai,Yuan Ping,Hong Yuan,Fuqiang Hu
Responsive drug release in tumor mitochondria is a pre-requisite for mitochondria-targeted drug delivery systems to improve the efficacy of this promising therapeutic modality. To this end, a photothermal stimulation strategy for mitochondria-responsive drug release along with heat shock is developed to maximize the antitumor effects with minimal side effects. Methods: This strategy relies on mitochondrial-targeted delivery of doxorubicin (DOX) through a photothermal and lipophilic agent IR-780 iodide (IR780)-modified glycolipid conjugates (CSOSA), which can synergistically triggers high-level reactive oxygen species (ROS) to kill tumor cells. Results: Specifically, upon laser irradiation, the photothermal conversion by IR780-CSOSA can not only weaken the hydrophobic interaction between the core of micelles and DOX and trigger unexpected micelle swelling to release DOX in mitochondria for the amplification of ROS, but also induce mitochondria-specific heat shock to promote the fast evolution of ROS at the same locus to eradicate cancer cells in a more effective way. Furthermore, IR780-CSOSA micelles may independently realize the real-time diagnosis and imaging on multiple tumor models. Deep penetration into tumors by IR780-CSOSA/DOX micelles can be manipulated under laser irradiation. Conclusion: Such multifunctional IR780-CSOSA/DOX micelles with integration of mitochondria-responsive drug release and heat shock are demonstrated to be superior to the non-mitochondria-responsive therapy. This study opens up new avenues for the future cancer diagnosis and treatment.
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JOURNAL OF CHROMATOGRAPHY A
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2019
·
IF 3.858
Xiaolan Wang,Xufeng Meng,Qiuhua Wu,Chun Wang,Zhi Wang
A solid phase extraction (SPE) method with a porous organic polymer as adsorbent was established for the extraction of five carbamate pesticides (metolcarb, carbaryl, isoprocarb, bassa and diethofencarb) from milk, white wine and juice samples prior to high performance liquid chromatography-diode array detection. The main factors affecting the SPE were optimized, including sample volume, sample loading rate, sample solution pH, desorption conditions and cartridge reusability. Under the optimal conditions, a good linearity existed in the range from 1.0 to 320.0 ng mL −1 for milk and white wine samples and from 0.5 to 160.0 ng mL −1 for juice sample with the correlation coefficients between 0.9956 and 0.9998. The method recoveries of the carbamates were 82.0%–110.0%. The limits of detection were 0.12-0.40 ng mL -1 for milk and white wine samples, and 0.06-0.20 ng mL −1 for juice sample. The adsorption mechanism of the adsorbent was investigated by the extraction of different types of organic compounds (carbamates, benzoylurea insecticides, endocrine disrupting chemicals and polycyclic aromatic hydrocarbons) with the adsorbent. The results showed that the existence of the hydrogen-bonding between the adsorbent and the analytes is favorable for the adsorption and strong hydrophobicity seems to have an adverse effect.
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APPLIED PHYSICS B-LASERS AND OPTICS
·
2018
·Vol. 125
·期 1
·1-12
·
IF 1.881
Zhang Yiran,Wang Lijun,Liu Peng,Li Youping,Zhan Reggie,Huang Zhen,Lin He
The laser-induced fluorescence technique is widely used in the measurement of polycyclic aromatic hydrocarbons (PAHs) in sooting flames. One of the main limitations is the absence of the PAH fluorescence spectra at elevated temperatures. In this study, fluorescence spectra and photophysical properties of five typical PAHs were experimentally studied in the temperature range of 673–1373 K in an optical cell. The experimental results indicated that the fluorescence spectra of PAHs were greatly sensitive to PAH structures and were likely to shift to the red and became broader as temperature increases. Further, we also observed that absorption cross sections of PAHs increased linearly as a function of temperature. Fluorescence quantum yields, which were calculated using integral fluorescence intensities and absorption cross sections, decreased monotonically with increasing temperature. However, the descent gradients of different PAHs were quite different, e.g., naphthalene, fluoranthene and fluorene were more sensitive to temperature, and their fluorescence production was much lower at elevated temperature compared with phenanthrene and pyrene. In order to investigate the fluorescence quantum yields at higher temperatures (up to 2000 K), which cannot be measured in the optical cell, a multistep decay model was established and optimized based on experimental results. At temperatures between 1373 and 2000 K, the extrapolation results indicated that fluorescence quantum yields of phenanthrene and pyrene would be two orders of magnitude higher than those of naphthalene, fluorene and fluoranthene. This contributed to explaining that the PAH fluorescence signals emitted from phenanthrene and pyrene were stronger than those emitted from naphthalene in flames, although the concentrations of phenanthrene and pyrene were much lower than that of naphthalene in flames.
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JOURNAL OF MOLECULAR LIQUIDS
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2018
·
IF 4.513
Yan Li,Lu Lai,Ping Mei,Wei-Xing Zhang,Yu-Hang Li,Li Cheng,Yan-Qun Wang,Yi Liu
The synergistic effect of sulfonate Gemini surfactant (SGS12) with different kinds of conventional surfactants on surface properties and solubility enhancement was investigated. Results indicated that the absolute values of β m were in the following order: SGS12/BQAS > SGS12/DTAB > SGS12/rhamnolipid > SGS12/SDS > SGS12/Triton X-100. The cationic/anionic surfactant mixtures formed micelles at a low concentration, which resulted from the strong attraction among different charged hydrophilic headgroups. Moreover, the molar solubilization ratio and ln K m values indicated that the solubility of SGS12/BQAS, SGS12/Triton X-100, and SGS12/DTAB mixtures toward pyrene was higher than that of either component. The micelle's hydrodynamic sizes of cationic/anionic surfactant mixtures were larger than that of either component, so the hydrophobic space to dissolve additional pyrene was large. Furthermore, dynamic surface tension determination suggested that cationic/anionic surfactant mixtures had a relatively short induction period, and the n values for SGS12/BQAS and SGS12/DTAB mixtures were slightly larger than those for either component. At the total surfactant concentration of 1 μmol·L −1 , the plots of γ t versus t 1/2 for all surfactant mixtures were linear, indicating that the initial adsorption was controlled by diffusion. The values of D t →0 for SGS12/BQAS, SGS12/DTAB, and SGS12/rhamnolipid mixtures were higher than those of either pure component. Therefore, the mixture of these surfactants accelerated the adsorption process and lowered the adsorption energy barrier, and the mixing of cationic and anionic surfactants was beneficial for dynamic surface activity. In conclusion, the cationic/anionic surfactant mixtures demonstrated good potential for the remediation of polycyclic aromatic hydrocarbon-contaminated soils from equilibrium and dynamic viewpoints.
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Polymer Chemistry
·
2018
·Vol. 10
·期 4
·512-525
·
IF 4.927
Yanan Tan,Xiqin Yang,Suhuan Dai,Keke Lian,Lijuan Wen,Yun Zhu,Tingting Meng,Xuan Liu,Hong Yuan,Fuqiang Hu
The selective delivery of DOX into tumor mitochondria in vitro and in vivo, which can be achieved with the aid of a skeletal polymer with positively charged and lipophilic character, may significantly promote its antitumor efficiency as well as eliminate its severe cardiotoxicity. In this study, we developed a polycationic glycolipid polymer (CSOSA) as a skeletal nanomaterial, which was further modified with lipophilic (4-carboxybutyl) triphenylphosphonium bromide (CTPP) cations to form the mitochondria-targeted polymer C-P-CSOSA. In vitro, C-P-CSOSA polymer micelles were internalized more by tumor cells than normal cells and exhibited rapid lysosomal escape capability due to their strong proton buffering ability. When loaded with DOX, the C-P-CSOSA/DOX micelles could selectively target mitochondria in tumor cells and remarkably activate the mitochondrial apoptosis signaling pathway. Importantly, the C-P-CSOSA polymer exhibited significant mitochondrial co-localization in vivo. Moreover, C-P-CSOSA/DOX demonstrated the highest tumor-inhibition rate (75.0%), when compared with CSOSA/DOX (44.8%) and DOX·HCl (55.0%). Meaningfully, C-P-CSOSA/DOX did not cause obvious organ damage and exhibited the longest survival time, indicating the low toxicity of this polymer. This study opens up new avenues for the future clinical translation of mitochondrial-targeted drug delivery systems.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 12
·1-7
·
IF 5.705
Pang Yachao,Zang Xiaohuan,Wang Mengting,Chang Qingyun,Zhang Shuaihua,Wang Chun,Wang Zhi
A fibrous magnetic boron nitride nanocomposite was synthesized and is shown to be a viable adsorbent for the magnetic solid phase extraction of pesticides prior to their quantitation by gas chromatography with electron capture detection. The optimum conditions were obtained by both single factor optimization and response surface analysis (Box-Behnken design). Under the optimized conditions, the response to the ten pesticides (dicofol, α-endosulfan, p,p’ -DDE, nitrofen, β -endosulfan, p,p’ -DDD, p,p’ -DDT, bifenthrin, permethrin and fenvalerate) is linear in the 0.03–40 ng·mL −1 concentration range with the coefficients of determination ranging from 0.9970 to 0.9992. The relative standard deviations at concentration levels of 0.5 ng·mL −1 , 20 ng·mL −1 and 40 ng·mL −1 were below 8.7%. The recoveries of the analytes from spiked tea water and tea beverage samples varied between 84.5% and 122%, with relative standard deviations ranging from 4.8 to 12%. The limits of detection are between 0.01 and 0.05 ng·mL −1 . The adsorbent can be reused over 50 times without significant loss of extraction efficiency.
A novel fibrous-shape magnetic boron nitride nanocomposite (Fe 3 O 4 @ f -BN) was used as the adsorbent for the magnetic solid phase extraction (MSPE) of ten pesticides from tea water and tea beverage samples prior to their determination by gas chromatography (GC).
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危险品化学品经营许可证（带存储）